##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 953018 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2429996075625 33.0 31.0 34.0 31.0 34.0 2 32.45761884875207 34.0 31.0 34.0 31.0 34.0 3 32.53712101974989 34.0 31.0 34.0 31.0 34.0 4 36.166664218304376 37.0 35.0 37.0 35.0 37.0 5 36.020594574289255 37.0 35.0 37.0 35.0 37.0 6 36.13229865542938 37.0 35.0 37.0 35.0 37.0 7 36.09645358219887 37.0 35.0 37.0 35.0 37.0 8 36.08848416294341 37.0 35.0 37.0 35.0 37.0 9 37.74567741637619 39.0 38.0 39.0 35.0 39.0 10-11 37.702189255606925 39.0 37.0 39.0 35.0 39.0 12-13 37.627937772424026 39.0 37.0 39.0 35.0 39.0 14-15 38.8137689949193 40.0 38.0 41.0 35.0 41.0 16-17 38.793924668789046 40.0 38.0 41.0 35.0 41.0 18-19 38.83954762659258 40.0 38.0 41.0 35.0 41.0 20-21 38.784902803514726 40.0 38.0 41.0 35.0 41.0 22-23 38.643729184548455 40.0 38.0 41.0 34.5 41.0 24-25 38.555666314802025 40.0 38.0 41.0 34.0 41.0 26-27 38.44178756329891 40.0 38.0 41.0 34.0 41.0 28-29 38.31423016144501 40.0 38.0 41.0 34.0 41.0 30-31 38.277993175365 40.0 37.5 41.0 34.0 41.0 32-33 38.10737834962194 40.0 37.0 41.0 34.0 41.0 34-35 37.944072409964974 40.0 37.0 41.0 33.5 41.0 36-37 37.73807682541148 40.0 37.0 41.0 33.0 41.0 38-39 37.54746290206481 40.0 36.0 41.0 33.0 41.0 40-41 37.30096755780059 40.0 36.0 41.0 33.0 41.0 42-43 37.015414714097744 40.0 36.0 41.0 32.0 41.0 44-45 36.593561191918724 39.0 35.0 41.0 31.0 41.0 46-47 36.05848577886252 39.0 35.0 41.0 29.5 41.0 48-49 35.558702458925225 39.0 35.0 41.0 27.5 41.0 50-51 35.20746145403339 39.0 35.0 40.5 26.5 41.0 52-53 34.92555072412064 38.5 35.0 40.0 26.0 41.0 54-55 34.6701363457983 38.0 34.5 40.0 25.5 41.0 56-57 34.34829510040734 38.0 34.0 40.0 24.0 41.0 58-59 34.0426691835831 37.5 34.0 40.0 23.0 41.0 60-61 33.66384370494576 37.0 33.5 40.0 22.5 41.0 62-63 33.27027139046692 36.5 33.0 39.5 21.5 41.0 64-65 32.83655502834154 36.0 33.0 39.0 19.5 40.5 66-67 32.39612210892135 35.0 32.5 39.0 18.0 40.0 68-69 31.945872480897528 35.0 32.0 38.0 16.0 40.0 70-71 31.51947444854137 35.0 31.0 37.5 11.5 39.5 72-73 31.09092797827533 35.0 31.0 37.0 7.5 39.0 74-75 30.622428957270483 34.5 31.0 36.0 7.0 39.0 76-77 29.45500976896554 33.5 29.5 35.0 3.5 37.0 78-79 29.99011141447486 34.0 30.0 35.0 2.0 37.0 80-81 29.807391360918682 34.0 30.0 35.0 2.0 37.0 82-83 29.47252832580287 34.0 30.0 35.0 2.0 36.5 84-85 29.09396044985509 34.0 29.5 35.0 2.0 36.0 86-87 28.708658703193436 34.0 29.0 35.0 2.0 36.0 88-89 28.29078044695903 34.0 29.0 35.0 2.0 35.0 90-91 27.811250154771475 33.0 27.0 35.0 2.0 35.0 92-93 27.38920251243943 33.0 26.5 35.0 2.0 35.0 94-95 26.9505875020199 33.0 25.5 35.0 2.0 35.0 96-97 26.43758512431035 33.0 25.0 35.0 2.0 35.0 98-99 25.676544409444524 32.5 23.5 34.5 2.0 35.0 100-101 23.38530174666166 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3752545984889224 1101 2 0.3218906351864703 1101 3 0.2417455919734408 1101 4 0.1561926689862645 1101 5 0.10262917489582435 1101 6 0.14117280556477851 1101 7 0.15567928370690964 1101 8 0.13097722541284895 1101 9 0.31835659324652 1101 10-11 0.2817222195209368 1101 12-13 0.3220106640099871 1101 14-15 0.448299717031432 1101 16-17 0.2283433832314543 1101 18-19 0.344532125880761 1101 20-21 0.4119505237381631 1101 22-23 0.4077279658760986 1101 24-25 0.41367066267046226 1101 26-27 0.3771596245205586 1101 28-29 0.30917906560225106 1101 30-31 0.312076103636052 1101 32-33 0.5548270884600726 1101 34-35 0.41943266277602476 1101 36-37 0.5217912731032541 1101 38-39 0.6134344304205257 1101 40-41 0.37079291287192007 1101 42-43 0.4067813271606937 1101 44-45 0.2620641675000428 1101 46-47 0.37725005114825194 1101 48-49 0.43810536323144333 1101 50-51 0.4248597071299187 1101 52-53 0.53691334226626 1101 54-55 0.4886516685770559 1101 56-57 0.5871128484584247 1101 58-59 0.6389778184865946 1101 60-61 0.7444887211347719 1101 62-63 0.6872866321557964 1101 64-65 0.6670241638494616 1101 66-67 0.6475927494866269 1101 68-69 0.6007459739732184 1101 70-71 0.6488354915405523 1101 72-73 0.5629201531363428 1101 74-75 0.43601888143822265 1101 76-77 0.4527821625855566 1101 78-79 0.40548067625280737 1101 80-81 0.4107890747014018 1101 82-83 0.2957665679360453 1101 84-85 0.5445507727238983 1101 86-87 0.8038588394813999 1101 88-89 0.6983885163434742 1101 90-91 0.7687774092000623 1101 92-93 1.084577060164417 1101 94-95 0.9607404150932233 1101 96-97 1.193106187854685 1101 98-99 1.3114141153109848 1101 100-101 2.1204065625612465 1102 1 0.17074310590904673 1102 2 0.23270352789501914 1102 3 0.23452102351433268 1102 4 0.14773128784642608 1102 5 0.06815143528482537 1102 6 0.03956905437237879 1102 7 -0.06021581188669245 1102 8 0.06878652188513712 1102 9 0.17890726972408544 1102 10-11 0.1628381723891934 1102 12-13 0.20379130106181975 1102 14-15 0.17692980330228636 1102 16-17 0.3113068670274899 1102 18-19 0.24379720387385362 1102 20-21 0.25276705896082063 1102 22-23 0.350699107908774 1102 24-25 0.31501712398293336 1102 26-27 0.2123231719113079 1102 28-29 0.20932827432577739 1102 30-31 0.173846051998936 1102 32-33 0.22468991197578703 1102 34-35 0.32122286889156726 1102 36-37 0.38174742857889754 1102 38-39 0.4151510398370206 1102 40-41 0.340760831876743 1102 42-43 0.34945651492184027 1102 44-45 0.40373333466308026 1102 46-47 0.435280973704522 1102 48-49 0.434530026634782 1102 50-51 0.4031766715422549 1102 52-53 0.36114225636413266 1102 54-55 0.261095676118849 1102 56-57 0.2547890438822904 1102 58-59 0.1979227284463363 1102 60-61 0.2734702204473223 1102 62-63 0.2655163404411738 1102 64-65 0.33986407087577675 1102 66-67 0.29677422879520776 1102 68-69 0.34672092123886955 1102 70-71 0.5616715470560507 1102 72-73 0.5126053145191243 1102 74-75 0.4594742837792829 1102 76-77 0.31456405361765505 1102 78-79 0.6173413889017354 1102 80-81 0.5273084733282936 1102 82-83 0.5675421592101344 1102 84-85 0.40100100836769315 1102 86-87 0.3197743122543528 1102 88-89 0.3379124815485106 1102 90-91 0.7479732794250502 1102 92-93 1.0891693896865497 1102 94-95 0.9673066439117797 1102 96-97 1.2217977042303225 1102 98-99 1.226299831430751 1102 100-101 1.792768016402082 1103 1 0.07991249134838796 1103 2 0.1864497684344002 1103 3 0.1674417014526881 1103 4 0.07900260326025688 1103 5 -0.06022027562976717 1103 6 0.06343838331160612 1103 7 0.005492713649310588 1103 8 0.06633372465757503 1103 9 0.017929172565935403 1103 10-11 0.08451627285236185 1103 12-13 0.033395469794122334 1103 14-15 0.14454257138271487 1103 16-17 0.16404185003811023 1103 18-19 -0.01783174621561301 1103 20-21 -0.026173508904626885 1103 22-23 0.06023464238564458 1103 24-25 0.11800132126432317 1103 26-27 0.029971823069558923 1103 28-29 0.11410867146331327 1103 30-31 0.16030609194246637 1103 32-33 0.3425009025848027 1103 34-35 0.2589133993788053 1103 36-37 -0.047888688683222824 1103 38-39 0.023621806932240474 1103 40-41 0.20605449867088055 1103 42-43 0.16574459937927344 1103 44-45 -0.0031909976834683107 1103 46-47 0.2593185439165282 1103 48-49 0.22661277175318872 1103 50-51 0.13357003191380556 1103 52-53 0.06908233162416622 1103 54-55 0.017520399559465716 1103 56-57 0.08009747310056525 1103 58-59 0.02333384936758165 1103 60-61 0.10181690763658935 1103 62-63 0.14075733150659886 1103 64-65 -0.033216066424202495 1103 66-67 -0.001217420520106316 1103 68-69 -0.1882119632412227 1103 70-71 -0.16602900865950687 1103 72-73 -0.2095085764813085 1103 74-75 -0.2355046034361088 1103 76-77 -0.29175333535333436 1103 78-79 0.08395517524319018 1103 80-81 0.1993406831120943 1103 82-83 -0.024959457889092818 1103 84-85 -0.12635236283173157 1103 86-87 0.08636669465464664 1103 88-89 0.19321790477210143 1103 90-91 0.3282797649765996 1103 92-93 0.398006227128338 1103 94-95 0.34076253054643146 1103 96-97 0.526421427666218 1103 98-99 0.738986781479305 1103 100-101 1.2721509848454353 1104 1 -0.017860795181412925 1104 2 0.11885828495052664 1104 3 -0.0031214054631618637 1104 4 0.0051498370574591945 1104 5 -0.08114774403044578 1104 6 -0.05128499640902362 1104 7 0.0034022792366883436 1104 8 0.012086489164587988 1104 9 0.004359848303479907 1104 10-11 0.06922958580550898 1104 12-13 0.032197632376274044 1104 14-15 0.18314893302478197 1104 16-17 0.14698962463501886 1104 18-19 0.04134828242765565 1104 20-21 -0.04565879338715462 1104 22-23 0.06911320757066619 1104 24-25 -0.133344511510181 1104 26-27 -0.1254841953311967 1104 28-29 -0.009857095360750634 1104 30-31 0.039711844336984825 1104 32-33 -0.15352287841906787 1104 34-35 0.15487227825231997 1104 36-37 0.23945200433586677 1104 38-39 0.18044678238196354 1104 40-41 0.24331926698877737 1104 42-43 0.4432420595631257 1104 44-45 0.39490880360192904 1104 46-47 0.2819818745212004 1104 48-49 0.2991081430442435 1104 50-51 0.5101561644405592 1104 52-53 0.5081899504559573 1104 54-55 0.43823959532803514 1104 56-57 0.7081388933010047 1104 58-59 0.5309544540211135 1104 60-61 0.5716990817500474 1104 62-63 0.4561446862283489 1104 64-65 0.49999887062381276 1104 66-67 0.449590310387336 1104 68-69 0.393465672039639 1104 70-71 0.8116186822275182 1104 72-73 0.9388883717311067 1104 74-75 0.7313575404307926 1104 76-77 0.4865160901934402 1104 78-79 0.6266834388105877 1104 80-81 0.7008364382661121 1104 82-83 0.8144019883955274 1104 84-85 0.6739359968709806 1104 86-87 0.4660117832899928 1104 88-89 0.6816796626177783 1104 90-91 0.4387451949570682 1104 92-93 0.6066704302889612 1104 94-95 0.5084154475194964 1104 96-97 0.7462245938418803 1104 98-99 0.8007112511240706 1104 100-101 1.5087369786265477 1105 1 0.22569210985157184 1105 2 0.22481528205511836 1105 3 0.1896781828153422 1105 4 0.16093692047427766 1105 5 0.08751349325549285 1105 6 0.10917716882173067 1105 7 0.1462382074368591 1105 8 0.10137426598639365 1105 9 0.19824766991178677 1105 10-11 0.09937364904089208 1105 12-13 0.11016149674389197 1105 14-15 0.15623539060922553 1105 16-17 0.19485617658539667 1105 18-19 0.16216300382813387 1105 20-21 0.1684132028727774 1105 22-23 0.195983927808264 1105 24-25 0.18918781137690388 1105 26-27 0.11661943433946931 1105 28-29 0.15382459049732233 1105 30-31 0.2605481732584565 1105 32-33 0.211832408155594 1105 34-35 0.2230766146853469 1105 36-37 0.2342206270724816 1105 38-39 0.2944028837565966 1105 40-41 0.14763998545848978 1105 42-43 0.08706525504047846 1105 44-45 0.22902040055488015 1105 46-47 0.12316384520568846 1105 48-49 0.16134998227642683 1105 50-51 0.4001365605260645 1105 52-53 0.3071036814462502 1105 54-55 0.3728257720146999 1105 56-57 0.3860532568023629 1105 58-59 0.41476767075240417 1105 60-61 0.34241656930607434 1105 62-63 0.4088784304974453 1105 64-65 0.465453944659032 1105 66-67 0.43670476346169806 1105 68-69 0.40151270571986686 1105 70-71 0.3883984544449639 1105 72-73 0.6275852810698801 1105 74-75 0.5152799733590818 1105 76-77 0.30009464924679463 1105 78-79 0.47965604440261345 1105 80-81 0.5278041959161577 1105 82-83 0.4291296747014677 1105 84-85 0.7377991859053594 1105 86-87 0.6502528289368641 1105 88-89 0.76297815677 1105 90-91 0.8090474252460993 1105 92-93 1.1232001838453627 1105 94-95 1.2520178534187423 1105 96-97 1.45736700311976 1105 98-99 1.584740150201231 1105 100-101 2.2082356841396056 1106 1 -0.02521052406378743 1106 2 0.1448169731725244 1106 3 -0.15400760757065513 1106 4 -0.1254369010282801 1106 5 -0.10204385676352956 1106 6 -0.008338579709111116 1106 7 -0.19514766327721134 1106 8 -0.19407240039841867 1106 9 -0.03247147776503567 1106 10-11 0.03772396401151212 1106 12-13 -0.04381474305655075 1106 14-15 0.05395660990487272 1106 16-17 -0.06673854712830263 1106 18-19 0.04089297465635866 1106 20-21 -0.010139200627840239 1106 22-23 -0.04478468505268296 1106 24-25 -0.03091534678485175 1106 26-27 -0.1543356242154772 1106 28-29 0.10012738506883778 1106 30-31 -0.0374351692350956 1106 32-33 -0.059854058677366595 1106 34-35 -0.5031061105503554 1106 36-37 -0.20763452872171229 1106 38-39 -0.21422181501311144 1106 40-41 -0.1821532754386439 1106 42-43 -0.11909041231673001 1106 44-45 -0.03725871837295358 1106 46-47 -0.20361922670908683 1106 48-49 -0.10811689296138383 1106 50-51 -0.08333276683198676 1106 52-53 0.02447991279110795 1106 54-55 -0.059888894201002074 1106 56-57 -0.10289106315704544 1106 58-59 -0.10550882694344921 1106 60-61 -0.3909829994589842 1106 62-63 0.0757325293432558 1106 64-65 -0.4671212043706845 1106 66-67 -0.37927206822984516 1106 68-69 -0.6498930356237231 1106 70-71 -0.18672098072182308 1106 72-73 0.09131926830194104 1106 74-75 -0.12608475838742095 1106 76-77 -0.21940816223589366 1106 78-79 -0.17863254478269397 1106 80-81 -0.1618377099731596 1106 82-83 -0.33376298162119866 1106 84-85 -0.19226888157022515 1106 86-87 -0.17138501827068353 1106 88-89 0.17500200152542433 1106 90-91 0.24986677162921822 1106 92-93 0.4145797128691662 1106 94-95 0.008036117486724947 1106 96-97 0.3825688802600773 1106 98-99 0.4032409746209673 1106 100-101 1.1541706606753515 1107 1 0.1302004243188577 1107 2 0.243538060362809 1107 3 0.21170491322434515 1107 4 0.16617842500787816 1107 5 0.02988222580200528 1107 6 0.17129261752715053 1107 7 0.2096545513696526 1107 8 0.1744427468579488 1107 9 0.22801426724334561 1107 10-11 0.31127419720964866 1107 12-13 0.2427534040723529 1107 14-15 0.3406154542749391 1107 16-17 0.16818610062322392 1107 18-19 0.37071880326962514 1107 20-21 0.28082571996714023 1107 22-23 0.3910768406873615 1107 24-25 0.3129693713019748 1107 26-27 0.3269880979339348 1107 28-29 0.33028838492829493 1107 30-31 0.3440341587555409 1107 32-33 0.3166906542454342 1107 34-35 0.2780675934596033 1107 36-37 0.3647663824995746 1107 38-39 0.1533528590487805 1107 40-41 0.15992321747062732 1107 42-43 0.21605958948087078 1107 44-45 0.2371372053171612 1107 46-47 0.46013995332248925 1107 48-49 0.24331040380325675 1107 50-51 0.4364024625911753 1107 52-53 0.3910430266316709 1107 54-55 0.40128860111705933 1107 56-57 0.41241879457084707 1107 58-59 0.4675228795047204 1107 60-61 0.22762693049986638 1107 62-63 0.1913192179297809 1107 64-65 0.3451722755598894 1107 66-67 0.5981622947486827 1107 68-69 0.6191615559445367 1107 70-71 0.574491976400445 1107 72-73 0.6289332935272149 1107 74-75 0.5919081034945748 1107 76-77 0.5037364663629873 1107 78-79 0.7028825403746666 1107 80-81 0.5476606607448673 1107 82-83 0.5268312732396829 1107 84-85 0.4205094609090949 1107 86-87 0.5773239759541084 1107 88-89 0.5209237306233838 1107 90-91 0.7515017732470639 1107 92-93 0.856263838073847 1107 94-95 0.9632512905632105 1107 96-97 1.0596768965888508 1107 98-99 1.2856434219757134 1107 100-101 2.0417256918392503 1108 1 0.09941907556838459 1108 2 0.07956596812149996 1108 3 -0.08952235568204259 1108 4 -0.0906000408125891 1108 5 -0.08121798870541852 1108 6 0.03306608647650222 1108 7 0.10888743047028271 1108 8 0.16726655500300325 1108 9 0.013226642049154691 1108 10-11 0.036610513099070374 1108 12-13 0.06465386515435512 1108 14-15 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-0.006874376618803524 2316 62-63 0.2013374493239226 2316 64-65 0.20872293996168878 2316 66-67 -0.0745169170446971 2316 68-69 -0.0192936345815653 2316 70-71 0.20315983975333296 2316 72-73 -0.05770156859284725 2316 74-75 -0.26068565365843455 2316 76-77 -0.06690817806458682 2316 78-79 -0.0655586754264128 2316 80-81 -0.2578165881618517 2316 82-83 -0.43240108065818106 2316 84-85 -0.45223621114266166 2316 86-87 -0.2427122860478903 2316 88-89 -0.1576686370885767 2316 90-91 -0.38094789967209053 2316 92-93 -0.24920718645821793 2316 94-95 -0.2426028341893236 2316 96-97 -0.5533396763106246 2316 98-99 -0.5180819666461289 2316 100-101 -1.0007827713024113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 7.0 5 16.0 6 40.0 7 114.0 8 291.0 9 541.0 10 1051.0 11 1631.0 12 2717.0 13 3644.0 14 4801.0 15 7215.0 16 10289.0 17 15036.0 18 13751.0 19 7785.0 20 5940.0 21 5705.0 22 5760.0 23 6064.0 24 6779.0 25 7887.0 26 9239.0 27 11308.0 28 13921.0 29 17024.0 30 21400.0 31 26563.0 32 34331.0 33 45572.0 34 64712.0 35 97856.0 36 150330.0 37 207023.0 38 136770.0 39 9901.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44087511547829 19.260624002687496 15.547891996304694 20.75060888552952 2 16.04250916561912 21.048290798285027 39.298313358194704 23.610886677901153 3 19.82764229007217 25.757855570408957 30.10520262996082 24.30929950955806 4 13.369226362174022 17.232326390819892 35.767987349648536 33.630459897357554 5 13.30446700486306 38.27618485923992 32.83289502129294 15.586453114604081 6 31.224069220098677 36.668667328424014 16.27912589269038 15.828137558786928 7 28.002409188493814 35.05914893527719 19.272773441844752 17.66566843438424 8 28.310797907279824 33.229487795613515 18.709720068246348 19.749994228860317 9 28.182573676467808 16.132224155262545 19.48105911955493 36.20414304871471 10-11 25.707856514777266 27.082227198227105 25.082474832584484 22.127441454411144 12-13 27.28865332471479 24.888035895670864 26.32594041452952 21.497370365084826 14-15 23.638063499325302 25.518772992745152 26.08402989240497 24.759133615524576 16-17 21.51863868258522 28.786759536546004 27.078239865354064 22.616361915514712 18-19 21.708614108023145 25.72973438067277 31.496571943027305 21.06507956827678 20-21 22.576068867534506 24.594865994136523 32.08260494555192 20.746460192777054 22-23 22.432298858776175 24.73421111171738 31.996396687554828 20.837093341951622 24-25 22.007371361865662 25.116144856442073 31.96563528809958 20.910848493592688 26-27 21.088587418736584 25.46457410913891 31.873884111851407 21.572954360273098 28-29 21.36009536281522 25.739355629428157 31.76479970450959 21.135749303247035 30-31 21.321849754410486 25.680184845327396 31.33645606093516 21.661509339326955 32-33 20.9737381665404 25.59568654526987 31.535710763070586 21.894864525119146 34-35 21.786367099047446 25.651771530023566 31.317351823365353 21.244509547563634 36-37 22.324931074193284 25.134743066103194 31.125871245806085 21.414454613897433 38-39 21.63458612534076 25.596998167925474 31.013265226889736 21.755150479844033 40-41 21.885840561248582 25.618876033820975 31.097838655723187 21.397444749207256 42-43 21.859868908473096 25.769003129322233 30.469230430192606 21.901897532012065 44-45 21.71412292317669 26.146568060624247 30.383686352199014 21.75562266400005 46-47 22.543173371331914 26.615394462643938 29.198609050406183 21.64282311561796 48-49 22.36358599732639 26.83595692841059 28.581097104147034 22.21935997011599 50-51 22.489554237170754 27.263283589606914 28.486817667662102 21.76034450556023 52-53 22.8746991137628 26.93207263661337 28.383356872587928 21.8098713770359 54-55 22.203672963154947 27.570937799705774 28.317513415276522 21.907875821862756 56-57 22.45744571456153 27.740661771341152 27.8847828687391 21.91710964535822 58-59 22.29197140033032 26.940047302359453 27.93006008281061 22.83792121449962 60-61 21.913856821172317 27.5454398552808 28.446209830244552 22.09449349330233 62-63 22.199790560094353 27.595963559974734 28.638651106275013 21.565594773655903 64-65 21.952890711403143 27.60010828756645 28.448675680837088 21.998325320193324 66-67 21.90121277877228 27.64758902769937 28.35360927075879 22.09758892276956 68-69 22.62465137069814 27.489984449401796 28.113949579126523 21.771414600773543 70-71 22.39821283543438 27.780692494790234 27.607873093687633 22.213221576087754 72-73 22.182133162367514 27.870331798908097 27.47694951926356 22.47058551946083 74-75 22.160925691291084 28.306405706086196 27.292415931501786 22.24025267112094 76-77 22.38567372284679 27.56002509921114 28.010803573489696 22.04349760445238 78-79 22.306189389917083 27.92497098690686 27.609814295217927 22.15902532795813 80-81 22.492020087763294 28.239707959345996 27.26291633526334 22.00535561762737 82-83 22.365982120981116 28.35538524254302 27.222875994277118 22.055756642198745 84-85 22.182681088250355 28.593486363292957 27.201589480134647 22.02224306832204 86-87 22.472398212835433 28.541958284103764 26.679034393893925 22.306609109166878 88-89 21.980539716983312 28.507331445995774 26.613348331301196 22.898780505719724 90-91 21.989226070453967 28.58979262782826 26.892009379310633 22.52897192240714 92-93 22.2289204035698 27.979580165688173 27.257435768288396 22.534063662453633 94-95 21.694081328999033 27.808866149432642 27.608607602374775 22.888444919193553 96-97 22.06595258431635 27.55818882749329 27.6760250068729 22.699833581317456 98-99 21.618412741051024 27.87136390441274 27.108783291745976 23.401440062790265 100-101 21.25081002515997 28.151620968567087 27.73384475326699 22.863724253005948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 798.0 1 555.5 2 385.5 3 509.5 4 493.0 5 481.5 6 842.5 7 1758.0 8 2649.5 9 3074.5 10 2879.5 11 2432.5 12 2379.5 13 2470.0 14 2548.5 15 2583.0 16 2644.0 17 2713.5 18 2769.5 19 2950.0 20 3095.0 21 3326.0 22 3717.0 23 4265.0 24 4871.0 25 5583.0 26 6657.0 27 7772.0 28 8823.0 29 10093.5 30 11606.0 31 13344.5 32 16072.5 33 17067.0 34 16722.0 35 17737.5 36 19630.0 37 21448.5 38 22802.5 39 23815.5 40 24870.5 41 26174.0 42 26536.5 43 27564.5 44 28432.5 45 29433.0 46 30202.0 47 31245.5 48 33890.5 49 33297.5 50 31732.0 51 31277.5 52 30229.0 53 30931.5 54 33571.5 55 33133.5 56 30104.5 57 28217.5 58 27061.0 59 24200.0 60 19306.0 61 16364.0 62 14360.0 63 10110.5 64 6305.5 65 4579.0 66 3390.0 67 2705.5 68 2123.0 69 1598.5 70 1215.5 71 801.0 72 548.5 73 452.0 74 361.0 75 273.0 76 182.0 77 112.0 78 65.0 79 39.0 80 24.0 81 11.0 82 7.0 83 5.0 84 3.5 85 2.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04805785410139158 2 0.0 3 0.0 4 1.0492981244845324E-4 5 0.012591577493814387 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035676136232474097 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.295788746907194E-4 24-25 5.771139684664927E-4 26-27 0.0022559909676417443 28-29 0.002728175123659784 30-31 5.246490622422661E-4 32-33 0.0 34-35 0.0 36-37 5.246490622422662E-5 38-39 0.0 40-41 0.0 42-43 0.01007326199505151 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0492981244845324E-4 74-75 5.246490622422662E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5739471867267984E-4 84-85 2.0985962489690648E-4 86-87 0.0 88-89 0.0 90-91 0.0072926219651675 92-93 0.0024133856863144242 94-95 0.0 96-97 0.0 98-99 4.1971924979381295E-4 100-101 0.012119393337796347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 953018.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.415726107135 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36085134209978 33.8362658200898 2 14.512990325694561 13.762879485572663 3 5.627009617652907 8.004262404985338 4 2.774723452099843 5.26262108911241 5 1.5817763686027688 3.7500537528203743 6 0.9512463193905027 2.706242096438421 7 0.6254539522901791 2.0759447306091348 8 0.44684744690683803 1.6950076923365742 9 0.3419540391517368 1.4592599155482544 >10 1.6185610902520429 13.593919078010522 >50 0.0960945894174709 3.116582049464658 >100 0.053756091607113886 4.90734533529006 >500 0.005375609128789973 1.6841751697999743 >1k 0.002911788278094569 2.4260066467234718 >5k 4.479674273991644E-4 1.7194347331984858 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8642 0.9068034391795328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7548 0.7920102243609249 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4807 0.5043976084397147 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2494 0.26169495224644235 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2104 0.2207723253915456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.20860046714752503 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1579 0.16568417385610765 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1446 0.15172850880046337 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1385 0.14532779024110773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.13525452824605622 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1285 0.1348348089962624 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1248 0.13095240593566962 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 1153 0.12098407375306656 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1060 0.11122560119536042 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1005 0.10545446151069549 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0492981244845322E-4 0.0 18-19 0.0 0.0 0.0 2.0985962489690645E-4 0.0 20-21 0.0 0.0 0.0 3.672543435695863E-4 0.0 22-23 0.0 0.0 0.0 5.246490622422661E-4 0.0 24-25 0.0 0.0 0.0 7.345086871391726E-4 0.0 26-27 0.0 0.0 0.0 0.001521482280502572 0.0 28-29 0.0 0.0 0.0 0.003672543435695863 0.0 30-31 0.0 0.0 0.0 0.009128893683015431 0.0 32-33 0.0 0.0 0.0 0.015634542054819532 0.0 34-35 0.0 0.0 0.0 0.0210908923021391 0.0 36-37 0.0 0.0 0.0 0.02586519876854372 0.0 38-39 0.0 0.0 0.0 0.029065558048221543 0.0 40-41 0.0 0.0 0.0 0.032265917327899366 0.0 42-43 0.0 0.0 0.0 0.037040223794303995 0.0 44-45 0.0 0.0 0.0 0.043650801978556544 0.0 46-47 0.0 0.0 0.0 0.05152053791219054 0.0 48-49 0.0 0.0 0.0 0.05996738781429102 0.0 50-51 0.0 0.0 0.0 0.06951600074710027 0.0 52-53 0.0 0.0 0.0 0.09664035726502543 0.0 54-55 0.0 0.0 0.0 0.12895873949914902 0.0 56-57 0.0 0.0 0.0 0.1652119897000896 0.0 58-59 0.0 0.0 0.0 0.2158930891126925 0.0 60-61 0.0 0.0 0.0 0.2900260016075247 0.0 62-63 0.0 0.0 0.0 0.34023491686410956 0.0 64-65 0.0 0.0 0.0 0.39028643740202174 0.0 66-67 0.0 0.0 0.0 0.4836215055749209 0.0 68-69 0.0 0.0 0.0 0.6006182464549463 0.0 70-71 0.0 0.0 0.0 0.6985702263755773 0.0 72-73 0.0 0.0 0.0 0.8104778713518528 0.0 74-75 0.0 0.0 0.0 0.9969381480727542 0.0 76-77 0.0 0.0 0.0 1.1409543156582562 0.0 78-79 0.0 0.0 0.0 1.2646665645349826 0.0 80-81 0.0 0.0 0.0 1.4187035292093118 0.0 82-83 0.0 0.0 0.0 1.6874287788898006 0.0 84-85 0.0 0.0 0.0 1.9659125011279954 0.0 86-87 0.0 0.0 0.0 2.3053079794925173 0.0 88-89 0.0 0.0 0.0 2.734261052781794 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 49.68814 1 GTATCAA 8270 0.0 39.53231 1 AGGTAAG 555 0.0 35.947273 2 TATCAAC 10410 0.0 31.1659 2 ATCAACG 10630 0.0 30.4762 3 TCAACGC 10650 0.0 30.463568 4 CAACGCA 10810 0.0 29.968733 5 GGTAAGG 730 0.0 29.932613 3 AAGGTAA 630 0.0 29.416653 1 AACGCAG 11115 0.0 29.14332 6 GTAAGGT 785 0.0 29.045662 4 TAAGGTG 855 0.0 28.88996 5 ACGCAGA 12390 0.0 25.88004 7 CGCAGAG 12495 0.0 25.586523 8 GAGTACT 7535 0.0 25.40705 12-13 GTACTTT 7755 0.0 24.529282 14-15 GCAGAGT 13300 0.0 23.752125 9 GGTGTGC 1075 0.0 23.4207 8 GTGTGCA 1090 0.0 23.098396 9 AGTACTT 7665 0.0 22.961937 12-13 >>END_MODULE