FastQCFastQC Report
Thu 26 May 2016
SRR937803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589712
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75951.2879168136310606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.9582643731177253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.6550655235097811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.2453740130775701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.18551428493908892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10910.18500556203706217No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.1229413679897984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7010.1188715847735844No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.10191415470602598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.050.52791
GTATCAA49050.042.640941
ATCAACG62650.033.3702933
TATCAAC62650.033.2944532
TCAACGC63450.033.027244
CAACGCA64000.032.7434165
AACGCAG64900.032.2051776
TAACGCT600.003941759531.679194
GGTTGCG600.00394504231.6738077
GCACCGT1354.2171632E-728.149716
ACGCAGA74050.028.0380577
CGCAGAG74650.027.6170718
GTGTACG700.00835824927.162831
CGCGATA350.00839285827.14205722-23
GTACATG42300.026.5206851
GCAGAGT76650.026.4649059
TACATGG43600.025.6100142
GAGTACT46900.025.22209412-13
ACATGGG43300.024.2511773
GTACTTT49550.023.86912314-15