##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937803_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589712 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7878269392517 33.0 31.0 34.0 30.0 34.0 2 31.897112488808098 33.0 31.0 34.0 30.0 34.0 3 31.878767940961012 33.0 31.0 34.0 30.0 34.0 4 35.50372385164284 37.0 35.0 37.0 33.0 37.0 5 35.504612420978376 37.0 35.0 37.0 33.0 37.0 6 35.591982187915455 37.0 35.0 37.0 33.0 37.0 7 35.54625139050926 37.0 35.0 37.0 33.0 37.0 8 35.53191557913015 37.0 35.0 37.0 33.0 37.0 9 37.15482642374583 39.0 37.0 39.0 34.0 39.0 10-11 37.03188590362753 39.0 37.0 39.0 33.0 39.0 12-13 36.90528088287164 39.0 37.0 39.0 33.0 39.0 14-15 37.975830574924714 40.0 38.0 41.0 33.0 41.0 16-17 37.91671019073718 40.0 38.0 41.0 33.0 41.0 18-19 37.934276392544156 40.0 38.0 41.0 33.0 41.0 20-21 37.8930460970779 40.0 38.0 41.0 33.0 41.0 22-23 37.80670818976043 40.0 38.0 41.0 33.0 41.0 24-25 37.795941069539026 40.0 38.0 41.0 33.0 41.0 26-27 37.68216519250075 40.0 37.0 41.0 33.0 41.0 28-29 37.5313254605638 40.0 37.0 41.0 32.0 41.0 30-31 37.20871798437203 39.0 36.5 41.0 32.0 41.0 32-33 36.961313319043875 39.0 35.5 41.0 31.0 41.0 34-35 36.89758305749247 39.0 35.0 41.0 31.0 41.0 36-37 36.71446231380742 39.0 35.0 41.0 31.0 41.0 38-39 36.51875576552622 39.0 35.0 41.0 30.5 41.0 40-41 36.22183286078628 39.0 35.0 41.0 30.0 41.0 42-43 35.86539022438072 39.0 35.0 41.0 29.5 41.0 44-45 35.477563624277614 39.0 35.0 40.5 27.5 41.0 46-47 34.90490358005263 38.5 35.0 40.0 24.5 41.0 48-49 34.39610352171908 38.0 34.0 40.0 22.0 41.0 50-51 33.41646176438668 37.5 33.0 39.5 19.0 40.5 52-53 33.47053900887212 38.0 33.5 39.5 18.0 40.5 54-55 33.73030479284803 38.0 34.0 40.0 16.5 41.0 56-57 33.483372391947256 38.0 33.5 40.0 13.5 41.0 58-59 33.22795110155466 38.0 33.0 40.0 9.5 41.0 60-61 32.88608676777817 37.0 33.0 40.0 7.5 41.0 62-63 32.50454459125811 36.5 32.5 40.0 4.5 41.0 64-65 32.10325887891038 36.0 32.0 39.0 2.0 41.0 66-67 31.660280781127057 35.5 31.0 39.0 2.0 40.5 68-69 31.15561748785848 35.0 31.0 39.0 2.0 40.0 70-71 30.700407147895923 35.0 30.5 38.0 2.0 40.0 72-73 30.24893592126326 35.0 30.0 37.0 2.0 39.5 74-75 29.743356926771035 34.5 29.0 37.0 2.0 39.0 76-77 29.275269962286675 34.0 29.0 36.0 2.0 39.0 78-79 28.746418590769732 34.0 28.5 36.0 2.0 38.0 80-81 28.202369970426243 34.0 27.0 35.0 2.0 37.0 82-83 27.72200073934395 34.0 26.0 35.0 2.0 37.0 84-85 27.231329021624113 33.0 26.0 35.0 2.0 36.0 86-87 26.72651650297094 33.0 25.0 35.0 2.0 36.0 88-89 26.288741792603847 33.0 24.5 35.0 2.0 35.5 90-91 25.758881453997883 32.0 21.5 35.0 2.0 35.0 92-93 25.159231964077378 32.0 19.0 34.5 2.0 35.0 94-95 24.450852280435196 31.5 12.0 34.0 2.0 35.0 96-97 23.677518856662232 31.0 2.0 34.0 2.0 35.0 98-99 22.69863764006837 31.0 2.0 34.0 2.0 35.0 100-101 20.071838456738206 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23779594607530186 1101 2 0.31164969931734987 1101 3 0.2505517330771774 1101 4 0.1611908907892854 1101 5 0.11119253620669411 1101 6 0.2528962853664325 1101 7 0.23104248825597296 1101 8 0.32077882303737226 1101 9 0.3834516456934338 1101 10-11 0.2868129293072954 1101 12-13 0.3040688475082547 1101 14-15 0.3944663269140918 1101 16-17 0.38875554601207085 1101 18-19 0.269387513312914 1101 20-21 0.28933243591975355 1101 22-23 0.3429591614901142 1101 24-25 0.1353694789200901 1101 26-27 0.22731514292252797 1101 28-29 0.29376900209290824 1101 30-31 0.41152723712983175 1101 32-33 0.3297043903444816 1101 34-35 0.09613125453987692 1101 36-37 0.3505265303543226 1101 38-39 0.2609458101492592 1101 40-41 0.5237719893957049 1101 42-43 0.6435935121785974 1101 44-45 0.8294877049917773 1101 46-47 0.8925473323615165 1101 48-49 0.760493428895856 1101 50-51 0.9628982998335971 1101 52-53 0.9929727097117791 1101 54-55 0.9498829426096833 1101 56-57 0.6958024237835687 1101 58-59 0.6571326422615869 1101 60-61 0.9083249431687079 1101 62-63 0.9717140353474534 1101 64-65 0.6188940560980392 1101 66-67 0.5597388996917552 1101 68-69 0.4092526279588995 1101 70-71 0.5335454768560091 1101 72-73 0.6717091638413102 1101 74-75 0.9827067383248327 1101 76-77 0.8367612711311203 1101 78-79 1.0282384101680009 1101 80-81 0.9816241168499822 1101 82-83 0.8822380501045295 1101 84-85 1.0771026320540251 1101 86-87 0.993449541670504 1101 88-89 1.1891817646328278 1101 90-91 1.2365311815519817 1101 92-93 1.2395201288590911 1101 94-95 1.3911401825995497 1101 96-97 1.668031954405972 1101 98-99 1.7472748553509838 1101 100-101 1.9967422189720807 1102 1 0.0831731630577508 1102 2 0.2993889439827697 1102 3 0.27684030802833703 1102 4 0.20312994104658344 1102 5 0.20419322253570016 1102 6 0.1666564056137645 1102 7 0.05370097280425057 1102 8 -0.006098566303720077 1102 9 0.218252874253416 1102 10-11 0.16683268067607315 1102 12-13 0.40342506468635975 1102 14-15 0.45910237067766246 1102 16-17 0.4520274720500268 1102 18-19 0.48592353937478805 1102 20-21 0.4965044187894776 1102 22-23 0.5114350763107893 1102 24-25 0.5404922572792898 1102 26-27 0.4803019163376874 1102 28-29 0.6227294465608111 1102 30-31 0.7614183872122879 1102 32-33 0.757726023683297 1102 34-35 0.9184030967276797 1102 36-37 0.9882744705909019 1102 38-39 0.8042132641791895 1102 40-41 0.7084892125271693 1102 42-43 0.9327212532244857 1102 44-45 0.7549006669828202 1102 46-47 0.8764908049378661 1102 48-49 1.0559518563018102 1102 50-51 0.8677648693997213 1102 52-53 1.0120121285991743 1102 54-55 0.9193591586697778 1102 56-57 1.1291268027489778 1102 58-59 1.0389652649390086 1102 60-61 1.0967905785820449 1102 62-63 1.052540145663194 1102 64-65 1.0217055205874992 1102 66-67 0.9943606268185228 1102 68-69 0.9664819084314438 1102 70-71 0.9831034002293926 1102 72-73 1.2767679676412769 1102 74-75 1.2359209940926341 1102 76-77 1.1253649487366069 1102 78-79 1.1012936301504226 1102 80-81 1.34822706775417 1102 82-83 1.3628236347108889 1102 84-85 1.5542255160023117 1102 86-87 1.720497892151709 1102 88-89 1.5530906412582013 1102 90-91 1.785633984942013 1102 92-93 1.5764820515741 1102 94-95 1.5858565361738997 1102 96-97 1.3789849579494202 1102 98-99 1.583083838497103 1102 100-101 1.5988476341390196 1103 1 0.2629790978121491 1103 2 0.3654839245150896 1103 3 0.30471680207931584 1103 4 0.2590426116860627 1103 5 0.17859305401966452 1103 6 0.23471853910836415 1103 7 0.23277984238778515 1103 8 0.32113172309539806 1103 9 0.3551551689254495 1103 10-11 0.42922846233908274 1103 12-13 0.41534179873679733 1103 14-15 0.6421902912033133 1103 16-17 0.5833629616638731 1103 18-19 0.2450967915305 1103 20-21 0.5311351444276937 1103 22-23 0.39216920828330615 1103 24-25 0.34202385063921525 1103 26-27 0.4504514743228327 1103 28-29 0.357819514306982 1103 30-31 0.4684433996131645 1103 32-33 0.5263817005673843 1103 34-35 0.6770293512548449 1103 36-37 0.7247711036676137 1103 38-39 0.7566329642478209 1103 40-41 0.7252456864649446 1103 42-43 0.7931179454177126 1103 44-45 0.7545592181525791 1103 46-47 0.6697460863528519 1103 48-49 0.9608727964582329 1103 50-51 0.864132941046222 1103 52-53 0.92431244048975 1103 54-55 0.7617015316202398 1103 56-57 0.8624297850751859 1103 58-59 0.9784591732200809 1103 60-61 0.9823185841811295 1103 62-63 1.1757403196152367 1103 64-65 1.189440135005139 1103 66-67 1.2962472590118814 1103 68-69 1.260007291159905 1103 70-71 1.42814542731427 1103 72-73 1.1849844835721584 1103 74-75 1.2005139395219757 1103 76-77 1.4222521585051027 1103 78-79 1.5053906763632305 1103 80-81 1.5750493173073998 1103 82-83 1.8122722203313053 1103 84-85 1.5791818581170496 1103 86-87 1.5089830824457309 1103 88-89 1.5399847819283252 1103 90-91 1.5045842251453188 1103 92-93 1.4238510269558056 1103 94-95 1.2415673952659496 1103 96-97 1.6042953196473562 1103 98-99 1.753402252271929 1103 100-101 1.7328586556316097 1104 1 0.2523400619350298 1104 2 0.10375709009556644 1104 3 0.22934941715086765 1104 4 0.1455916792047205 1104 5 0.13850348647980582 1104 6 0.2645217140541192 1104 7 0.25101708845571835 1104 8 0.20063177897875306 1104 9 0.2844646432749869 1104 10-11 0.3285112282957101 1104 12-13 0.3226457541225969 1104 14-15 0.38238468822184046 1104 16-17 0.4218985863435094 1104 18-19 0.014849632910390653 1104 20-21 -0.04788778316673614 1104 22-23 0.06049471152261532 1104 24-25 0.058584403152231346 1104 26-27 0.11905157683989387 1104 28-29 0.3147344058739918 1104 30-31 0.030790924751038062 1104 32-33 -0.05821414710613482 1104 34-35 -0.06673483637450062 1104 36-37 0.2790829867827469 1104 38-39 0.40556790445261726 1104 40-41 0.4258207069876221 1104 42-43 0.38832199676873813 1104 44-45 0.39864579496907027 1104 46-47 0.38940142090148555 1104 48-49 0.16704964224713592 1104 50-51 0.33770300143878984 1104 52-53 0.4588608836563708 1104 54-55 0.6074682706141061 1104 56-57 0.3075571413296885 1104 58-59 0.47758141903749873 1104 60-61 0.4936802497365562 1104 62-63 0.5848171264784909 1104 64-65 0.4622607802459946 1104 66-67 0.77458474826879 1104 68-69 0.6365464923746949 1104 70-71 0.5390103090535128 1104 72-73 0.6373207934974303 1104 74-75 0.834859468274928 1104 76-77 0.6828353110204333 1104 78-79 0.8502575005310469 1104 80-81 0.6683205751455183 1104 82-83 0.45967133159314955 1104 84-85 0.7583545145808266 1104 86-87 0.8596540681896911 1104 88-89 1.104418298070911 1104 90-91 1.0625892276697044 1104 92-93 1.0116168101514873 1104 94-95 1.0680871336547355 1104 96-97 0.859960736325263 1104 98-99 0.9447362844684548 1104 100-101 1.0424283922860873 1105 1 0.21614171255778913 1105 2 0.04606997603048413 1105 3 -0.022205766106711877 1105 4 0.006655108709949786 1105 5 0.13791070875542744 1105 6 0.11469085024651804 1105 7 0.14340389995240344 1105 8 -0.03488178624738936 1105 9 0.015261162594050859 1105 10-11 0.21000145776248047 1105 12-13 0.3056577515775487 1105 14-15 0.2909870602482343 1105 16-17 0.25146698007608137 1105 18-19 0.250698495189134 1105 20-21 0.1705736954360475 1105 22-23 0.21233049149451233 1105 24-25 0.19939314422051524 1105 26-27 0.03985166655760253 1105 28-29 0.13173202103078552 1105 30-31 0.2163005991581457 1105 32-33 0.13078810956891118 1105 34-35 0.015181781084741885 1105 36-37 0.16874965802684727 1105 38-39 0.253813373937156 1105 40-41 0.4193939894198877 1105 42-43 0.34243321926408044 1105 44-45 0.3270947771765833 1105 46-47 0.5773256918306586 1105 48-49 0.49700940660081727 1105 50-51 0.38774122301406777 1105 52-53 0.38274387528424825 1105 54-55 0.5391809633813054 1105 56-57 0.6858317232955145 1105 58-59 0.38206181159824837 1105 60-61 0.5469112316419569 1105 62-63 0.6904356980443822 1105 64-65 0.30358173619734075 1105 66-67 0.2067059770352131 1105 68-69 0.3689748669735984 1105 70-71 0.40152135211343776 1105 72-73 0.5448074159460816 1105 74-75 0.6030936772714206 1105 76-77 0.6484564917238025 1105 78-79 0.6798613693784041 1105 80-81 0.6610413104645438 1105 82-83 0.8911105226689848 1105 84-85 0.8250351424217008 1105 86-87 0.9136174395615022 1105 88-89 1.0300555913425384 1105 90-91 1.0219404679254929 1105 92-93 0.7403946810037603 1105 94-95 1.2245793085310304 1105 96-97 1.3542732743751813 1105 98-99 1.1784703721629377 1105 100-101 1.6559338829183687 1106 1 0.25437954346209324 1106 2 0.3133403979219551 1106 3 0.07827635345009298 1106 4 0.14794046598707666 1106 5 0.15789950353025262 1106 6 0.18721150988042723 1106 7 0.27214972563743345 1106 8 0.40355213609744567 1106 9 0.5949620125693542 1106 10-11 0.31962836799535665 1106 12-13 0.046350780010804726 1106 14-15 0.21011777067425186 1106 16-17 0.20617428042772445 1106 18-19 0.16785907638647046 1106 20-21 0.1943497826009093 1106 22-23 0.1694668192768347 1106 24-25 0.166536770142919 1106 26-27 0.21802764756769477 1106 28-29 0.5176064340465416 1106 30-31 0.4514444609800279 1106 32-33 0.5848282567112122 1106 34-35 0.6933393324481685 1106 36-37 0.5133527202969361 1106 38-39 0.590060434408521 1106 40-41 0.9933153022004859 1106 42-43 1.1606394986132926 1106 44-45 1.1995450355602344 1106 46-47 1.2163515744264402 1106 48-49 1.1274360160618642 1106 50-51 0.9740325146374289 1106 52-53 1.1948849579154057 1106 54-55 0.9128733799502982 1106 56-57 0.7038190584423205 1106 58-59 0.8271414645311523 1106 60-61 0.8124160933827369 1106 62-63 0.8576018081357191 1106 64-65 1.1199081915865428 1106 66-67 1.346188914868332 1106 68-69 1.0113696889800714 1106 70-71 1.0644007263900725 1106 72-73 1.1503116142195893 1106 74-75 1.259087735553333 1106 76-77 1.1397097814157284 1106 78-79 1.1680203213949767 1106 80-81 0.8760802707375994 1106 82-83 1.0459932732568937 1106 84-85 1.332294580243758 1106 86-87 1.1785767837609562 1106 88-89 1.4959939183729318 1106 90-91 1.4392263707201636 1106 92-93 1.3092699565269754 1106 94-95 1.7388751854504783 1106 96-97 1.519030629647169 1106 98-99 1.5446434928918684 1106 100-101 1.8576297790046468 1107 1 0.30256922005020215 1107 2 0.22056105990369446 1107 3 0.23735491799141428 1107 4 0.2247786842532591 1107 5 0.16604690625616314 1107 6 0.23243696346128928 1107 7 0.12437226131126522 1107 8 0.24354304132231164 1107 9 0.21112881748221923 1107 10-11 0.3566285237854956 1107 12-13 0.21465254128137445 1107 14-15 0.4408324263149055 1107 16-17 0.6400497914825038 1107 18-19 0.48180145088067405 1107 20-21 0.30468498401228317 1107 22-23 0.27980412596023285 1107 24-25 0.14279772282061742 1107 26-27 0.1683369120277618 1107 28-29 0.05991398060463382 1107 30-31 0.26185629429301116 1107 32-33 0.169108611575858 1107 34-35 0.269973339328196 1107 36-37 0.24174315562508042 1107 38-39 0.5909467539298632 1107 40-41 0.4951616257639486 1107 42-43 0.2880989826532243 1107 44-45 0.7055934820800047 1107 46-47 0.3900302004417071 1107 48-49 0.6133706936815599 1107 50-51 0.33477087564105545 1107 52-53 0.4553365808696128 1107 54-55 0.48910738514610586 1107 56-57 0.0855469815072567 1107 58-59 0.34881102945563214 1107 60-61 0.4853178740218169 1107 62-63 0.5512068338410927 1107 64-65 0.6047649699308728 1107 66-67 0.7657569972602509 1107 68-69 0.7211663618081694 1107 70-71 0.586057659682421 1107 72-73 0.6030882574896665 1107 74-75 0.3789956088496389 1107 76-77 0.36367282968119596 1107 78-79 0.7022773077401823 1107 80-81 0.7608818909549449 1107 82-83 0.9535234102258876 1107 84-85 0.7997807591329327 1107 86-87 1.1123175574063175 1107 88-89 0.9017303635790199 1107 90-91 1.058485820233635 1107 92-93 1.2557623628107955 1107 94-95 1.2397151889873328 1107 96-97 1.5650539813244393 1107 98-99 1.5726478213311417 1107 100-101 1.6146687560108681 1108 1 0.13812506900716315 1108 2 -0.08290309377996508 1108 3 0.10907890795708752 1108 4 0.09973329872381953 1108 5 -0.20303647108737977 1108 6 -0.020220152989729456 1108 7 0.21563430910635617 1108 8 -0.01462635397739831 1108 9 0.15634848344472374 1108 10-11 0.2107491172631839 1108 12-13 0.23868624209996625 1108 14-15 0.10993065710444938 1108 16-17 0.13685836357122838 1108 18-19 0.1822713974082788 1108 20-21 0.25682181725758824 1108 22-23 0.22862368959899015 1108 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-0.27311573665214084 2316 82-83 -0.5769283632469744 2316 84-85 -0.20045029447150498 2316 86-87 -0.26861888283994517 2316 88-89 -0.5003829582077017 2316 90-91 -0.7836809167800602 2316 92-93 -0.8307135421188363 2316 94-95 -0.1591706596161515 2316 96-97 -0.5556469661772709 2316 98-99 -0.7841280530339141 2316 100-101 -0.7736905086928729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2322.0 3 458.0 4 410.0 5 485.0 6 550.0 7 650.0 8 811.0 9 999.0 10 1273.0 11 1772.0 12 2702.0 13 3747.0 14 4240.0 15 5752.0 16 7791.0 17 10691.0 18 8899.0 19 5367.0 20 4805.0 21 4976.0 22 5316.0 23 5548.0 24 6184.0 25 6934.0 26 7772.0 27 9101.0 28 10577.0 29 12791.0 30 15332.0 31 18635.0 32 23952.0 33 30887.0 34 41659.0 35 60038.0 36 81269.0 37 103846.0 38 73594.0 39 7577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.51624590473308 18.767076009174076 15.313195472806749 20.40348261328609 2 15.42785270547043 20.440727804264505 41.26201463427071 22.869404855994357 3 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20.02116403913819 22-23 21.42448884265059 25.004182679037772 33.38253466103314 20.1887938172785 24-25 20.959865334506503 25.23636104212883 33.548235318097966 20.2555383052667 26-27 20.542749552860926 25.503997499757958 33.49186317289548 20.461389774485642 28-29 20.377836684114907 25.55659269212247 33.719329328419626 20.346241295342992 30-31 20.35383272065069 25.420185911243685 33.644601031481244 20.58138033662438 32-33 20.137856639287868 25.694931666256128 33.46357374013642 20.703637954319593 34-35 20.79867496230862 25.618669441318243 33.26209840103625 20.320557195336885 36-37 20.863562287852396 25.434358548874307 33.0486157169899 20.6534634462834 38-39 20.493409069100537 25.594279226530503 33.17926330011048 20.73304840425848 40-41 20.55673408936891 25.692329641474725 33.167791321952606 20.58314494720376 42-43 20.641028798650645 25.815596572596228 32.62887372807871 20.914500900674422 44-45 20.55033264056219 25.963375140245816 32.4768960681911 21.009396151000896 46-47 20.93288522771766 26.36722747814891 31.667121057311824 21.032766236821608 48-49 21.05945303210464 26.58552743332767 31.092152199762186 21.262867334805506 50-51 21.135789760667777 26.998108545856507 30.656929966935646 21.209171726540067 52-53 21.30876131903977 27.208253342620754 30.2925536433292 21.19043169501028 54-55 20.93760934331167 27.068563139711454 30.423683039429832 21.57014447754704 56-57 21.126600294158607 27.35972268927544 30.14658677509095 21.367090241475005 58-59 21.316006684737197 26.73512747875354 30.44170895578155 21.507156880727713 60-61 21.099254194571746 26.95963921460736 30.77765691591666 21.163449674904236 62-63 21.139496235052942 27.034247558325468 30.861950808994433 20.964305397627154 64-65 20.972713809197074 26.91526437000251 30.788082799475543 21.323939021324872 66-67 20.917512992085864 27.2473761081485 30.440711932339255 21.39439896742638 68-69 20.949269479140835 27.047190866196296 30.640093213170775 21.36344644149209 70-71 21.025814784715447 27.287889325659975 30.370819502013024 21.315476387611554 72-73 21.092457026304327 27.32577640516321 29.969625666269543 21.612140902262915 74-75 20.819480994803836 27.77999981317919 29.877997518679962 21.52252167333702 76-77 20.964251988831293 27.43245446578395 30.05648892300794 21.546804622376815 78-79 20.950883045325924 27.410498031449254 29.929529619979682 21.709089303245136 80-81 20.879500679347824 27.8257472826087 29.703040081521735 21.59171195652174 82-83 21.134809830444283 27.624326282027877 29.67509534165473 21.565768545873116 84-85 20.89031092271427 27.405286433389715 29.537063454862878 22.16733918903313 86-87 20.996305607986884 27.450326093140117 29.49238832976291 22.060979969110093 88-89 21.065252556988252 27.256284735342696 29.50465119241204 22.173811515257015 90-91 20.8788959834102 27.294875725103196 29.731460243836782 22.094768047649822 92-93 21.02837307012345 27.114054409314697 29.769051666601566 22.08852085396029 94-95 20.769058322619653 27.065730769296092 29.764304675014564 22.400906233069694 96-97 20.89497487351844 27.02671949539843 29.670816767744356 22.407488863338774 98-99 20.940961681350174 27.24857233839295 29.232056045005837 22.578409935251035 100-101 21.268893051025035 26.470138748563016 29.910070690278772 22.35089751013318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1226.0 1 783.5 2 515.0 3 658.0 4 485.0 5 397.0 6 771.0 7 1744.0 8 2995.5 9 3463.5 10 2789.0 11 2054.0 12 1868.5 13 1946.0 14 2043.0 15 2064.5 16 2067.5 17 2083.5 18 2066.5 19 2072.0 20 2196.0 21 2394.5 22 2676.5 23 3079.0 24 3520.0 25 4086.0 26 4754.5 27 5458.0 28 6164.0 29 6864.5 30 7715.5 31 8730.0 32 10054.0 33 11138.0 34 11792.5 35 12508.0 36 13758.5 37 14949.5 38 15499.5 39 15728.5 40 16106.0 41 17016.5 42 17632.0 43 18472.5 44 19621.5 45 20661.0 46 20606.5 47 20658.5 48 21669.5 49 21095.5 50 19911.0 51 18858.5 52 17751.5 53 17430.5 54 17277.5 55 16206.0 56 14449.5 57 12990.0 58 12025.5 59 10696.5 60 9049.5 61 7689.0 62 6365.0 63 4776.0 64 3491.5 65 2722.5 66 2016.0 67 1571.0 68 1170.0 69 749.0 70 549.0 71 440.5 72 349.0 73 273.0 74 225.5 75 180.5 76 123.5 77 76.5 78 58.0 79 42.5 80 27.0 81 23.0 82 15.0 83 8.5 84 9.5 85 7.5 86 4.0 87 1.0 88 1.5 89 4.5 90 3.5 91 2.0 92 1.5 93 1.5 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25995740293567027 2 0.23180806902352333 3 0.22994275171609194 4 0.233164663428928 5 0.23926933825324906 6 0.17211791518571776 7 0.17466152969585153 8 0.1748311039965271 9 0.1717787665843666 10-11 0.18152928887321268 12-13 0.1717787665843666 14-15 0.17355929674146023 16-17 0.1772899313563231 18-19 0.1717787665843666 20-21 0.16575887891038338 22-23 0.1652501560083566 24-25 0.16855685487153052 26-27 0.1643174973546409 28-29 0.1727114252380823 30-31 0.1770355699053097 32-33 0.17924003581409229 34-35 0.17796822855902542 36-37 0.15965420408606235 38-39 0.15439740076511924 40-41 0.1592302683343734 42-43 0.16728504761646365 44-45 0.1718635537347044 46-47 0.17152440513335324 48-49 0.17160919228369104 50-51 0.17152440513335324 52-53 0.1868708793444936 54-55 0.1644870716553165 56-57 0.15499091081748378 58-59 0.1539734650134303 60-61 0.15015804324822965 62-63 0.156941015275253 64-65 0.15533005941883496 66-67 0.15126027620262092 68-69 0.16177388284450714 70-71 0.15626271807255065 72-73 0.15134506335295872 74-75 0.1547365493664704 76-77 0.15668665382423963 78-79 0.16177388284450714 80-81 0.1546517622161326 82-83 0.15405825216376806 84-85 0.14990368179721628 86-87 0.14354464552188187 88-89 0.14998846894755405 90-91 0.15541484656917276 92-93 0.14905581029383835 94-95 0.1527864449087012 96-97 0.15109070190194535 98-99 0.15253208345768782 100-101 0.13650731204384514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.99363116312208 #Duplication Level Percentage of deduplicated Percentage of total 1 80.08974828278077 46.44695321858878 2 11.390841984384963 13.211925773596542 3 3.491612183183074 6.074738073485478 4 1.5616443386168253 3.622617031268875 5 0.8586694563989798 2.489867987272049 6 0.5293302052602896 1.8418668412418964 7 0.3551176790086621 1.441619458715263 8 0.24620840043167594 1.1422815331117506 9 0.21348398457494006 1.1142640314605445 >10 1.1509287406705653 12.355524960206616 >50 0.07807395265673102 3.115325036926334 >100 0.03111217055943245 3.076344703703736 >500 0.0014675552150673465 0.5361857918810315 >1k 0.001174044172053877 1.2757817492534385 >5k 5.870220860269385E-4 2.2547038092876646 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7595 1.2879168136310606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5651 0.9582643731177253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3863 0.6550655235097811 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1447 0.2453740130775701 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.18551428493908892 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1091 0.18500556203706217 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.1229413679897984 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 701 0.1188715847735844 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 601 0.10191415470602598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.4787150337792E-5 0.0 20-21 0.0 0.0 0.0 1.69574300675584E-4 0.0 22-23 0.0 0.0 0.0 5.08722902026752E-4 0.0 24-25 0.0 0.0 0.0 0.0012718072550668802 0.0 26-27 0.0 0.0 0.0 0.001865317307431424 0.0 28-29 0.0 0.0 0.0 0.0033914860135116805 0.0 30-31 0.0 0.0 0.0 0.005765526222969856 0.0 32-33 0.0 0.0 0.0 0.009580947988170496 0.0 34-35 8.4787150337792E-5 0.0 0.0 0.013820305505060096 0.0 36-37 1.69574300675584E-4 0.0 0.0 0.017127004368233986 0.0 38-39 1.69574300675584E-4 0.0 0.0 0.02187508478715034 0.0 40-41 1.69574300675584E-4 0.0 0.0 0.029251566866538244 0.0 42-43 1.69574300675584E-4 0.0 0.0 0.04188485226686925 0.0 44-45 1.69574300675584E-4 0.0 0.0 0.06079238679219687 0.0 46-47 1.69574300675584E-4 0.0 0.0 0.08012385706921345 0.0 48-49 1.69574300675584E-4 0.0 0.0 0.11946509482594894 0.0 50-51 1.69574300675584E-4 0.0 0.0 0.15371910356241691 0.0 52-53 1.69574300675584E-4 0.0 0.0 0.19153417261307215 0.0 54-55 1.69574300675584E-4 0.0 0.0 0.26029655153702147 0.0 56-57 1.69574300675584E-4 0.0 0.0 0.33024595056569983 0.0 58-59 1.69574300675584E-4 0.0 0.0 0.4172375668122745 0.0 60-61 1.69574300675584E-4 0.0 0.0 0.5015159942480397 0.0 62-63 1.69574300675584E-4 0.0 0.0 0.6243725750875003 0.0 64-65 1.69574300675584E-4 0.0 0.0 0.7160274846026535 0.0 66-67 1.69574300675584E-4 0.0 0.0 0.8399862983965054 0.0 68-69 1.69574300675584E-4 0.0 0.0 1.002862414195404 0.0 70-71 1.69574300675584E-4 0.0 0.0 1.124701549230811 0.0 72-73 1.69574300675584E-4 0.0 0.0 1.3116572157256425 0.0 74-75 1.69574300675584E-4 0.0 0.0 1.5360888026697777 0.0 76-77 1.69574300675584E-4 0.0 0.0 1.7254185093740673 0.0 78-79 1.69574300675584E-4 0.0 0.0 1.9097457742084272 0.0 80-81 1.69574300675584E-4 0.0 0.0 2.1696183899937598 0.0 82-83 1.69574300675584E-4 0.0 0.0 2.4621340586591423 0.0 84-85 2.54361451013376E-4 0.0 0.0 2.767452587025531 0.0 86-87 3.39148601351168E-4 0.0 0.0 3.171972081287137 0.0 88-89 3.39148601351168E-4 0.0 0.0 3.6408450226551263 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2700 0.0 50.5279 1 GTATCAA 4905 0.0 42.64094 1 ATCAACG 6265 0.0 33.370293 3 TATCAAC 6265 0.0 33.294453 2 TCAACGC 6345 0.0 33.02724 4 CAACGCA 6400 0.0 32.743416 5 AACGCAG 6490 0.0 32.205177 6 TAACGCT 60 0.0039417595 31.67919 4 GGTTGCG 60 0.003945042 31.673807 7 GCACCGT 135 4.2171632E-7 28.14971 6 ACGCAGA 7405 0.0 28.038057 7 CGCAGAG 7465 0.0 27.617071 8 GTGTACG 70 0.008358249 27.16283 1 CGCGATA 35 0.008392858 27.142057 22-23 GTACATG 4230 0.0 26.520685 1 GCAGAGT 7665 0.0 26.464905 9 TACATGG 4360 0.0 25.610014 2 GAGTACT 4690 0.0 25.222094 12-13 ACATGGG 4330 0.0 24.251177 3 GTACTTT 4955 0.0 23.869123 14-15 >>END_MODULE