FastQCFastQC Report
Thu 26 May 2016
SRR937803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589712
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66241.1232601676750684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55830.9467333206717856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.6589657324253195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.30421629541199774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.2397780611552758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.17567897549990505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.046.7242471
ATACCGT459.6246373E-442.2192086
GTATCAA52800.036.54371
ACAGCGC804.488434E-429.6904148
ACTTGAC2101.8189894E-1229.407658
TATCAAC68300.028.0322172
ATCAACG68350.028.011713
TCAACGC68650.027.8892974
CAACGCA70700.027.0134325
AACGCAG72500.026.4015646
CCAAGAC2850.025.0045743
GTCCTAC1151.2761843E-424.7955571
ATACGTC1151.2791282E-424.7871445
CGGTTAC1351.8189894E-1224.62891428-29
GTACATG47550.023.6874491
ATGGGAT11650.023.6523975
GAGTACT48750.023.38691312-13
CGCAGAG82000.023.3468048
ACGCAGA82050.023.3325777
CATCCCG1252.251303E-422.8041735
Produced by FastQC (version 0.11.5)