##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589712 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13552378109993 33.0 31.0 34.0 31.0 34.0 2 32.41019853759123 34.0 31.0 34.0 31.0 34.0 3 32.473894036411 34.0 31.0 34.0 31.0 34.0 4 36.12716715956263 37.0 35.0 37.0 35.0 37.0 5 36.053593957728516 37.0 35.0 37.0 35.0 37.0 6 36.114511829503215 37.0 35.0 37.0 35.0 37.0 7 36.05405011259734 37.0 35.0 37.0 35.0 37.0 8 36.042264698700386 37.0 35.0 37.0 35.0 37.0 9 37.71220188837941 39.0 37.0 39.0 35.0 39.0 10-11 37.63244261605665 39.0 37.0 39.0 35.0 39.0 12-13 37.5224151789348 39.0 37.0 39.0 35.0 39.0 14-15 38.66894263640557 40.0 38.0 41.0 34.0 41.0 16-17 38.64744231082291 40.0 38.0 41.0 34.5 41.0 18-19 38.70137626502428 40.0 38.0 41.0 35.0 41.0 20-21 38.632816527389636 40.0 38.0 41.0 34.0 41.0 22-23 38.55944851045935 40.0 38.0 41.0 34.0 41.0 24-25 38.5520584624359 40.0 38.0 41.0 34.0 41.0 26-27 38.385149869766934 40.0 38.0 41.0 34.0 41.0 28-29 38.2086348929647 40.0 38.0 41.0 34.0 41.0 30-31 38.153569030306315 40.0 37.0 41.0 34.0 41.0 32-33 37.96827519195811 40.0 37.0 41.0 33.5 41.0 34-35 37.7879007040725 40.0 36.0 41.0 33.0 41.0 36-37 37.595773021407055 40.0 36.0 41.0 33.0 41.0 38-39 37.41765217597742 40.0 36.0 41.0 33.0 41.0 40-41 37.17604779960388 40.0 35.5 41.0 33.0 41.0 42-43 36.86816445993976 40.0 35.0 41.0 31.5 41.0 44-45 36.429351954852535 39.0 35.0 41.0 30.5 41.0 46-47 35.84536265159943 39.0 35.0 41.0 29.0 41.0 48-49 35.3298957796348 39.0 35.0 41.0 26.5 41.0 50-51 34.98039042786988 39.0 35.0 40.0 25.0 41.0 52-53 34.709810042868384 38.5 35.0 40.0 24.0 41.0 54-55 34.44416257427355 38.0 34.0 40.0 23.0 41.0 56-57 34.150191958108365 38.0 34.0 40.0 22.5 41.0 58-59 33.871461832216404 38.0 34.0 40.0 22.0 41.0 60-61 33.520192059852945 37.0 33.0 40.0 20.5 41.0 62-63 33.132658823290015 36.5 33.0 40.0 18.5 41.0 64-65 32.70453712998888 36.0 32.5 39.0 17.0 41.0 66-67 32.272535576688284 35.5 32.0 39.0 12.5 40.0 68-69 31.835000813956643 35.0 31.5 38.5 8.0 40.0 70-71 31.43202614157419 35.0 31.0 38.0 7.0 40.0 72-73 30.95814482323575 35.0 31.0 37.0 4.0 39.5 74-75 30.491078696041455 34.5 30.0 36.5 2.0 39.0 76-77 29.28529858642863 33.5 28.5 35.0 2.0 37.5 78-79 29.841228430148952 34.0 30.0 36.0 2.0 38.0 80-81 29.65713432997802 34.0 30.0 35.0 2.0 37.0 82-83 29.289401436633476 34.0 29.5 35.0 2.0 37.0 84-85 28.907682902840712 34.0 29.0 35.0 2.0 36.0 86-87 28.54758085302656 34.0 29.0 35.0 2.0 36.0 88-89 28.121433004585292 34.0 28.5 35.0 2.0 35.5 90-91 27.582920137287353 33.0 27.0 35.0 2.0 35.0 92-93 27.13621225276067 33.0 26.0 35.0 2.0 35.0 94-95 26.664643927883443 33.0 25.0 35.0 2.0 35.0 96-97 26.089808245380794 33.0 24.5 35.0 2.0 35.0 98-99 25.216141947255608 32.0 21.0 34.0 2.0 35.0 100-101 22.612526114442304 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2734319697451113 1101 2 0.19463776886011175 1101 3 0.15855611430619376 1101 4 0.09824985906527672 1101 5 0.025672537529509043 1101 6 0.09254629313138452 1101 7 0.14425001894228018 1101 8 0.13297706280117438 1101 9 0.22113667304201812 1101 10-11 0.294340648743308 1101 12-13 0.15188471782605717 1101 14-15 0.29278946033520725 1101 16-17 0.1374003856996211 1101 18-19 0.13584925106656698 1101 20-21 0.21522236703606978 1101 22-23 0.15513166377212428 1101 24-25 0.21816255381608585 1101 26-27 -0.0049787411554405026 1101 28-29 0.058382896123610806 1101 30-31 0.2161447116736923 1101 32-33 0.33227138256464883 1101 34-35 0.10972471978179499 1101 36-37 0.2325156560827466 1101 38-39 0.2661359868950086 1101 40-41 0.30077459534657436 1101 42-43 0.6307290167447448 1101 44-45 0.5601882965608169 1101 46-47 0.06886175252705584 1101 48-49 -0.18554112256957467 1101 50-51 0.035217915036589886 1101 52-53 -0.028754410317752388 1101 54-55 0.04866742328183449 1101 56-57 -0.10272665058667485 1101 58-59 0.007318042176848394 1101 60-61 0.06438674910157971 1101 62-63 -0.13112448465034277 1101 64-65 0.06770717932872827 1101 66-67 -0.0989164586774649 1101 68-69 -0.23883250852727045 1101 70-71 -0.2661903375985588 1101 72-73 -0.17557358731814787 1101 74-75 -0.15318173278774694 1101 76-77 -0.06100446741510268 1101 78-79 -0.1613530293058325 1101 80-81 -0.15612363081404368 1101 82-83 -0.026297340325101004 1101 84-85 0.17941122485891725 1101 86-87 0.0877722033159607 1101 88-89 -0.041342993090406566 1101 90-91 0.08947451374244153 1101 92-93 0.09856366891096258 1101 94-95 -0.1721493319718057 1101 96-97 -0.15014060376328686 1101 98-99 -0.42313214822044287 1101 100-101 -0.48055875702421247 1102 1 0.22528335001175037 1102 2 0.2277508143907454 1102 3 0.1617750284156898 1102 4 0.12843280767134502 1102 5 -0.005437421073359872 1102 6 0.027941304979101744 1102 7 0.018530693345937266 1102 8 -0.018954751498696965 1102 9 0.2833457207838066 1102 10-11 0.17700789097397518 1102 12-13 0.25117494183017897 1102 14-15 0.24779764348877364 1102 16-17 0.2767088704031764 1102 18-19 0.19604667158750289 1102 20-21 0.19814876834288242 1102 22-23 0.19273612708458643 1102 24-25 0.08314051985988158 1102 26-27 0.16751033503329182 1102 28-29 0.1260173591565703 1102 30-31 0.2158970431736975 1102 32-33 0.24465729201269681 1102 34-35 0.34641350839556395 1102 36-37 0.19044384308721618 1102 38-39 0.2880628012414519 1102 40-41 0.1958850446281062 1102 42-43 0.49594145265372447 1102 44-45 0.39172247481381106 1102 46-47 0.07924985450125632 1102 48-49 0.09820079908953971 1102 50-51 0.30508884791610313 1102 52-53 0.26193309880483895 1102 54-55 0.0766128150949541 1102 56-57 0.16155194309101972 1102 58-59 0.07626771034765056 1102 60-61 -0.14261209873524194 1102 62-63 0.043330411732604546 1102 64-65 -0.4302027780319193 1102 66-67 -0.4279723991500717 1102 68-69 -0.6190906117126467 1102 70-71 -0.47246947164389397 1102 72-73 -0.7314239986497171 1102 74-75 -0.5406478433519375 1102 76-77 -0.5764569631993055 1102 78-79 -0.5571373853522026 1102 80-81 -0.5047616869812224 1102 82-83 -0.5048961421996161 1102 84-85 -0.4016454733706034 1102 86-87 -0.5898402723442011 1102 88-89 -0.6953485458337063 1102 90-91 -0.38671670186734985 1102 92-93 -0.6237952842664924 1102 94-95 -0.7189633368102655 1102 96-97 -0.8348135726486703 1102 98-99 -0.8760417484415584 1102 100-101 -0.8608129782581777 1103 1 0.30551872886706377 1103 2 0.24151748762591296 1103 3 0.22003876960766888 1103 4 0.18580300615334266 1103 5 -0.02414066296861961 1103 6 0.08422090570480378 1103 7 0.010658683996012996 1103 8 0.07221038742510899 1103 9 0.1482814740437064 1103 10-11 0.25624780209535913 1103 12-13 0.3188089902241771 1103 14-15 0.23862439133306879 1103 16-17 0.17153243818671626 1103 18-19 0.26828093606254555 1103 20-21 0.09489702032872316 1103 22-23 0.07605235966389756 1103 24-25 0.09643324173367063 1103 26-27 0.004831201803696672 1103 28-29 0.15750539655625317 1103 30-31 0.30782661977715975 1103 32-33 0.23705538397624792 1103 34-35 0.23422633328157616 1103 36-37 0.33855109539241823 1103 38-39 0.23464559493841364 1103 40-41 0.3343196121602361 1103 42-43 0.6084208128363713 1103 44-45 0.6016574466485309 1103 46-47 0.4882851681245626 1103 48-49 0.49730691662947635 1103 50-51 0.48701312441830424 1103 52-53 0.45498588917224225 1103 54-55 0.3333296741734202 1103 56-57 0.3402553888363329 1103 58-59 0.6252155043503791 1103 60-61 0.6394518150497817 1103 62-63 0.5282557006900532 1103 64-65 0.5454439362450572 1103 66-67 0.7412775006236529 1103 68-69 0.7329346385132887 1103 70-71 0.6151792539535599 1103 72-73 0.6416606602751784 1103 74-75 0.8807424819247913 1103 76-77 0.8073739907509712 1103 78-79 0.7161473863697161 1103 80-81 0.8187636277972246 1103 82-83 0.8262905368792524 1103 84-85 0.8742129052061145 1103 86-87 0.7495989361740136 1103 88-89 0.5494481816935277 1103 90-91 0.5213961902891775 1103 92-93 0.623223408782902 1103 94-95 0.6335817310378253 1103 96-97 0.0767902515782275 1103 98-99 0.5085961119483393 1103 100-101 0.5582585527236326 1104 1 0.12059104964527023 1104 2 -0.009832384613154943 1104 3 0.10173947373802861 1104 4 0.0046792495581442495 1104 5 0.05506725782345967 1104 6 -0.01221643363052749 1104 7 -0.017535435532714416 1104 8 -0.009221305763666976 1104 9 -0.032612968066906944 1104 10-11 -0.011435427885878369 1104 12-13 0.05224246397249033 1104 14-15 -0.004961547642302833 1104 16-17 -0.06961679351340422 1104 18-19 -0.063274085924661 1104 20-21 -0.0019353565640827242 1104 22-23 -0.15428440503632146 1104 24-25 -0.07125249336377948 1104 26-27 -0.1736082264131653 1104 28-29 -0.04937866281114367 1104 30-31 0.1417904073587124 1104 32-33 -0.143095038617588 1104 34-35 -0.030101004473607418 1104 36-37 -0.061251000426352675 1104 38-39 -0.29370943013201867 1104 40-41 -0.289262588410935 1104 42-43 -0.15295434180637812 1104 44-45 -0.4228676411268921 1104 46-47 -0.479510599385236 1104 48-49 -0.30031052514583934 1104 50-51 -0.2428544127441299 1104 52-53 -0.05051862196395973 1104 54-55 -0.08701630164643603 1104 56-57 -0.06688862451401434 1104 58-59 -0.08522389303428213 1104 60-61 -0.047006875583782914 1104 62-63 -0.11184388731468431 1104 64-65 -0.09224110512790418 1104 66-67 -0.1430111812612722 1104 68-69 -0.017236865597027418 1104 70-71 -0.18605326322575877 1104 72-73 -0.2732183811517359 1104 74-75 -0.2038051904368423 1104 76-77 -0.2522612643276716 1104 78-79 -0.11287939524966717 1104 80-81 -0.2046967855854902 1104 82-83 0.021398623009002193 1104 84-85 0.3198393576917695 1104 86-87 0.09591969125457922 1104 88-89 -0.26580624176360956 1104 90-91 -0.3538268614048583 1104 92-93 -0.2484809181309089 1104 94-95 -0.5580105208304182 1104 96-97 -0.8748099395814108 1104 98-99 -0.947765727038206 1104 100-101 -0.7217652651505624 1105 1 0.23975768198118175 1105 2 0.23344450895692148 1105 3 0.1659837622445508 1105 4 0.05333737473265643 1105 5 0.05029299398930931 1105 6 0.19862705016306847 1105 7 0.11192584624039625 1105 8 0.18617562643995456 1105 9 0.11588815111574746 1105 10-11 0.16139360952549708 1105 12-13 0.3706396296252521 1105 14-15 0.34143692715662866 1105 16-17 -0.06049625593954033 1105 18-19 0.27175747548390916 1105 20-21 -0.007609086211665783 1105 22-23 0.13874690919080734 1105 24-25 0.27525126242095155 1105 26-27 -0.015166064582558647 1105 28-29 -0.05743380258309827 1105 30-31 0.12836708166969402 1105 32-33 0.025956471249067192 1105 34-35 0.04178874344709271 1105 36-37 0.047734273967940055 1105 38-39 0.04005418201283106 1105 40-41 -0.011462686368027164 1105 42-43 -0.02530801267970162 1105 44-45 -0.16760130311999433 1105 46-47 -0.4209720825654628 1105 48-49 -0.5523875358982266 1105 50-51 -0.5460922608007834 1105 52-53 -0.6512370502561637 1105 54-55 -0.6912067137528268 1105 56-57 -0.7224435697450104 1105 58-59 -0.6196038566894515 1105 60-61 -0.36913922751523387 1105 62-63 -0.4889352025488378 1105 64-65 -0.5170830692133492 1105 66-67 -0.6483175718171097 1105 68-69 -0.7022544658313912 1105 70-71 -0.8092892345344005 1105 72-73 -0.5388835052230903 1105 74-75 -0.5226749761671492 1105 76-77 -0.8129248549585775 1105 78-79 -0.582914366846687 1105 80-81 -0.49419546499573386 1105 82-83 -0.6479870395517047 1105 84-85 -0.3147349180437118 1105 86-87 -0.3262456182028579 1105 88-89 -0.5175628321244474 1105 90-91 -0.23818027594187186 1105 92-93 -0.13913517079612348 1105 94-95 -0.24705855184710757 1105 96-97 -0.04970111108413633 1105 98-99 -0.17911497153269806 1105 100-101 0.0885046661421498 1106 1 0.13691656765102778 1106 2 0.13683630112947753 1106 3 0.16468631511920506 1106 4 0.057812092943358095 1106 5 0.10569988372841976 1106 6 0.1267035110811605 1106 7 0.11010131122038302 1106 8 0.011904887558557675 1106 9 0.11080156863570068 1106 10-11 0.029608735105107087 1106 12-13 -0.0073487375817720135 1106 14-15 0.059597869575043205 1106 16-17 0.2946835805526291 1106 18-19 0.25045648986640145 1106 20-21 0.4014148059822702 1106 22-23 0.284506984678643 1106 24-25 0.3031003014991782 1106 26-27 0.3774609709719243 1106 28-29 0.3417394434674321 1106 30-31 0.17331698989005417 1106 32-33 0.2468357865508395 1106 34-35 -0.13040128832561493 1106 36-37 0.1267292684759056 1106 38-39 0.20930468514218603 1106 40-41 -0.029969737550835873 1106 42-43 -0.005407680208875831 1106 44-45 0.3941509139664987 1106 46-47 0.3925014635197144 1106 48-49 0.5643716475947045 1106 50-51 0.6613721146922131 1106 52-53 0.8489748906805019 1106 54-55 0.2697205923488539 1106 56-57 0.5756817665960412 1106 58-59 0.5217570621390095 1106 60-61 0.33258799897038216 1106 62-63 0.4082126253330003 1106 64-65 -0.17798309943797364 1106 66-67 -0.46370595819096394 1106 68-69 0.1290774078739254 1106 70-71 -0.15492619998250134 1106 72-73 0.1415132482103516 1106 74-75 -0.15365263206352964 1106 76-77 -0.005232689032780513 1106 78-79 0.04129960872297289 1106 80-81 -0.09282171913319104 1106 82-83 -0.3403999226119119 1106 84-85 -0.47940381101169294 1106 86-87 -0.00916354730032154 1106 88-89 0.23420825692022973 1106 90-91 0.27956747379827007 1106 92-93 -0.19142376431920383 1106 94-95 0.04922013389792923 1106 96-97 -0.13467468297081453 1106 98-99 -0.13610201917702014 1106 100-101 -0.358358976588093 1107 1 0.26200762066598315 1107 2 0.2091397634598522 1107 3 0.13890936447941016 1107 4 0.052338385685644084 1107 5 -0.03892809365253669 1107 6 1.6661873374346214E-5 1107 7 0.17817317678613165 1107 8 0.10798529210762808 1107 9 0.12060792571509893 1107 10-11 0.10685748141561646 1107 12-13 0.170847802193137 1107 14-15 0.21264061922872912 1107 16-17 0.17909362017385888 1107 18-19 0.11907067350507106 1107 20-21 -0.001178468094671814 1107 22-23 0.09524302988304356 1107 24-25 0.05481715083327998 1107 26-27 0.1292072941143374 1107 28-29 -0.05229379456725525 1107 30-31 -0.02053014209786852 1107 32-33 -0.012133724899733522 1107 34-35 0.2668319631828098 1107 36-37 0.15433148995894186 1107 38-39 0.15587709176284648 1107 40-41 0.3417876172169727 1107 42-43 0.30151992790736415 1107 44-45 0.038538890336930365 1107 46-47 0.013944080120204205 1107 48-49 0.14438040552631293 1107 50-51 0.2697094129545192 1107 52-53 0.3999372105218484 1107 54-55 0.43960551818012306 1107 56-57 0.3682072088084283 1107 58-59 0.4148597699398806 1107 60-61 0.6763536569310418 1107 62-63 0.30355323729347816 1107 64-65 0.5282767163928312 1107 66-67 0.5451677633826009 1107 68-69 0.40746384318523 1107 70-71 0.6105254440244217 1107 72-73 0.7892843967204541 1107 74-75 0.584472581095266 1107 76-77 0.47422617184663096 1107 78-79 0.4850572666499531 1107 80-81 0.8471611860579884 1107 82-83 0.7092023915030943 1107 84-85 0.6859589905158927 1107 86-87 0.4128298084611792 1107 88-89 0.4969883107309876 1107 90-91 0.6907628893579165 1107 92-93 0.6836788676918744 1107 94-95 0.5270263562399151 1107 96-97 0.4159907935154017 1107 98-99 0.49705854149902606 1107 100-101 0.7467277816719395 1108 1 0.09174999589703958 1108 2 0.06431453900777484 1108 3 0.13279056991907368 1108 4 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-0.5271752269406882 2316 72-73 -0.36976417183879207 2316 74-75 -0.5461090918056044 2316 76-77 -0.41908710045458974 2316 78-79 -0.39879554310742193 2316 80-81 -0.7048986477289603 2316 82-83 -0.5689952448367244 2316 84-85 -0.7668569525466786 2316 86-87 -0.62647113612962 2316 88-89 -0.596803078792643 2316 90-91 -0.4175721420071561 2316 92-93 -0.030185804030075758 2316 94-95 0.1965014461143113 2316 96-97 0.10423398562101127 2316 98-99 0.41666644171297307 2316 100-101 0.7884447828508634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 16.0 6 35.0 7 97.0 8 194.0 9 372.0 10 677.0 11 1120.0 12 1706.0 13 2586.0 14 3293.0 15 4726.0 16 6803.0 17 10249.0 18 9128.0 19 5059.0 20 3832.0 21 3846.0 22 3764.0 23 4066.0 24 4406.0 25 5159.0 26 6116.0 27 7152.0 28 9018.0 29 10888.0 30 13426.0 31 17016.0 32 21452.0 33 28303.0 34 40150.0 35 58687.0 36 86891.0 37 123815.0 38 88949.0 39 6706.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19700402990542 19.979070272323913 15.596039294970081 20.22788640280058 2 15.871476246031962 21.526948747863365 39.58678134411374 23.014793661990936 3 19.28331117562471 26.718465963046366 30.37940554033155 23.61881732099737 4 12.53001465121958 17.805979868139023 36.53681797216268 33.12718750847872 5 12.81388453527109 38.954249696377566 32.85025137902257 15.381614389328774 6 30.319715386493744 37.056732778034025 16.959295384865833 15.664256450606398 7 27.523943891255392 35.38829123368695 19.646200179070462 17.441564695987193 8 27.557689177089834 34.262996174403774 18.809690153837806 19.36962449466858 9 27.872927802045744 16.48177415416339 20.088449955232385 35.55684808855848 10-11 25.138626990802287 27.984846840491628 25.47311229888488 21.4034138698212 12-13 26.632558667990512 25.63060087791072 27.185047198087826 20.551793256010942 14-15 23.193864123504355 26.223648153675015 26.998687494912772 23.58380022790786 16-17 20.88646661421168 29.870852212605474 27.456707681037525 21.78597349214532 18-19 20.85356919988062 26.68073568114605 32.1424695444556 20.323225574517732 20-21 21.894416257427356 25.534498195729437 32.64423990015465 19.926845646688555 22-23 21.41554443331844 25.821612166668505 32.603510711630044 20.159332688383007 24-25 21.174524470460952 26.057679100440723 32.727556379311224 20.0402400497871 26-27 20.778659196277466 26.329052103929758 32.57589375044514 20.31639494934764 28-29 20.43582660541591 26.491884953036 32.8478379941225 20.224450447425586 30-31 20.6224394280598 26.248575575874327 32.73487736386575 20.394107632200125 32-33 20.45769799495347 26.540921670239033 32.51943321485742 20.481947119950078 34-35 20.88188810809344 26.32564709553138 32.560046259869225 20.232418536505957 36-37 21.19102205822503 26.08569946007543 32.22344127302819 20.49983720867135 38-39 20.89545405214749 26.492423420245814 32.22505222888461 20.387070298722087 40-41 20.8630940268338 26.532294167921535 32.29467665133694 20.309935153907723 42-43 20.910170927342524 26.751057643141156 31.570128375702655 20.768643053813662 44-45 20.77641289307323 26.91347640882329 31.48121455897116 20.828896139132322 46-47 21.19568535149361 27.419655696339905 30.61511381827061 20.769545133895868 48-49 21.494983992186018 27.57074639824185 29.99243698618987 20.94183262338226 50-51 21.567985728626855 27.861735898201157 29.578590905391106 20.991687467780885 52-53 21.56281371245625 28.329930542366444 29.125912309737632 20.981343435439673 54-55 21.314847442221698 28.283260783672006 29.20939239729291 21.19249937681339 56-57 21.446909677944486 28.445071492525166 28.980332772607646 21.127686056922702 58-59 21.321509482594895 27.989255772309196 29.192385435602464 21.49684930949345 60-61 21.296497273245247 28.157049542827682 29.615812464389396 20.93064071953767 62-63 21.258597417044253 28.229796917817513 29.783351873456876 20.728253791681365 64-65 21.05180155737038 28.114825541959465 29.6671934774941 21.16617942317606 66-67 21.14201508532979 28.408019507827547 29.30888298016659 21.14108242667607 68-69 21.17686260411862 28.292963344819167 29.38841332718344 21.141760723878775 70-71 21.18228898174024 28.65280001085275 28.99271169655696 21.172199310850043 72-73 21.233585207694603 28.626685568548716 28.60777803402339 21.531951189733295 74-75 21.205690235233472 29.01848699025965 28.382159426974525 21.393663347532353 76-77 21.170503567843284 28.73080418916352 28.722834197031773 21.37585804596142 78-79 21.146678378598367 28.800923162492875 28.53553938193559 21.516859076973166 80-81 21.180593238733483 29.287177469680113 28.318314702770163 21.213914588816234 82-83 21.120604502054828 29.200189585193364 28.10608970364171 21.573116209110093 84-85 21.05239506742274 29.001953495943784 28.13737892394932 21.808272512684155 86-87 21.043746778088288 29.036970589033288 27.94270762677375 21.976575006104675 88-89 20.89740415660526 29.018147841658298 28.00451745937 22.079930542366444 90-91 21.006590529040047 28.942700047826282 28.0412934125237 22.00941601060997 92-93 21.07725596996449 28.779790470441004 28.08045419550892 22.06249936408559 94-95 20.808377648750575 28.65186735219904 28.198171310741515 22.34158368830887 96-97 21.104454377730146 28.669927015220992 27.923969666549098 22.301648940499767 98-99 20.86909998609467 28.96785155892298 27.436145714276023 22.726902740706322 100-101 20.849216532491617 28.79481606301323 27.851788656703253 22.5041787477919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1265.0 1 826.5 2 471.5 3 577.5 4 510.5 5 487.0 6 808.0 7 1611.5 8 2388.0 9 2671.5 10 2439.5 11 2041.0 12 2021.5 13 2178.0 14 2257.0 15 2282.5 16 2213.5 17 2176.5 18 2223.5 19 2243.0 20 2302.0 21 2504.5 22 2742.0 23 3135.5 24 3599.0 25 4039.0 26 4609.5 27 5223.0 28 5878.5 29 6744.5 30 7875.5 31 8712.5 32 9661.0 33 10660.0 34 11643.0 35 12634.0 36 13923.0 37 15131.0 38 15688.5 39 16024.5 40 16175.5 41 16902.0 42 17470.0 43 18310.0 44 19537.5 45 20519.5 46 20335.0 47 20125.5 48 21651.5 49 21258.5 50 20019.0 51 19070.0 52 17756.5 53 17333.0 54 17197.0 55 16332.5 56 14540.0 57 13111.5 58 12427.5 59 11362.0 60 9488.5 61 7831.5 62 6494.5 63 4839.5 64 3366.5 65 2621.0 66 1999.0 67 1589.5 68 1186.5 69 703.5 70 504.0 71 400.5 72 353.0 73 301.5 74 231.0 75 195.5 76 138.5 77 71.0 78 48.5 79 38.0 80 26.0 81 21.5 82 14.5 83 11.0 84 6.0 85 2.0 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02034891608107008 2 0.0 3 0.0 4 0.0 5 0.027810185310795776 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027979759611471366 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.2393575168896006E-4 24-25 1.69574300675584E-4 26-27 0.001017445804053504 28-29 8.478715033779201E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.001356594405404672 42-43 0.012802859701006593 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.69574300675584E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.93510052364544E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013226795452695554 92-93 0.0016957430067558402 94-95 0.0 96-97 0.0 98-99 0.0016957430067558402 100-101 0.02060327753208346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.7859644420047 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69076568990721 43.89840256691288 2 11.937795695637146 13.319228923854611 3 3.7986968155669105 6.3574189643751655 4 1.7392544286147813 3.8810394284121355 5 0.9481238222255467 2.6446000916645964 6 0.5682883345581641 1.9021507694680684 7 0.4104559298902628 1.6028375936907722 8 0.2822224711356083 1.2595242191604592 9 0.22895322452175054 1.1495138797847426 >10 1.2613134415454668 12.827397628508228 >50 0.09145237664755448 3.453040472393424 >100 0.03993419883717562 3.963556032789486 >500 9.145236374924974E-4 0.28280746910922056 >1k 0.0012193648499899967 1.3825765026205428 >5k 6.096824249949983E-4 2.0759054572557156 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6624 1.1232601676750684 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5583 0.9467333206717856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3886 0.6589657324253195 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1794 0.30421629541199774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1414 0.2397780611552758 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.17567897549990505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.4787150337792E-5 0.0 20-21 0.0 0.0 0.0 1.69574300675584E-4 0.0 22-23 0.0 0.0 0.0 5.08722902026752E-4 0.0 24-25 0.0 0.0 0.0 0.001187020104729088 0.0 26-27 0.0 0.0 0.0 0.0016957430067558402 0.0 28-29 0.0 0.0 0.0 0.0033914860135116805 0.0 30-31 0.0 0.0 0.0 0.005765526222969856 0.0 32-33 0.0 0.0 0.0 0.009580947988170496 0.0 34-35 0.0 0.0 0.0 0.013650731204384512 0.0 36-37 0.0 0.0 0.0 0.0169574300675584 0.0 38-39 0.0 0.0 0.0 0.021705510486474754 0.0 40-41 0.0 0.0 0.0 0.02899720541552487 0.0 42-43 0.0 0.0 0.0 0.04196963941720704 0.0 44-45 0.0 0.0 0.0 0.06104674824321024 0.0 46-47 0.0 0.0 0.0 0.08080215427191578 0.0 48-49 0.0 0.0 0.0 0.12014339202865128 0.0 50-51 0.0 0.0 0.0 0.15337995496106574 0.0 52-53 0.0 0.0 0.0 0.19127981116205878 0.0 54-55 0.0 0.0 0.0 0.2600421900860081 0.0 56-57 0.0 0.0 0.0 0.3299915891146865 0.0 58-59 0.0 0.0 0.0 0.4179158640149768 0.0 60-61 0.0 0.0 0.0 0.50304216295412 0.0 62-63 0.0 0.0 0.0 0.6262378923949318 0.0 64-65 0.0 0.0 0.0 0.7179775890604227 0.0 66-67 0.0 0.0 0.0 0.8426994872073148 0.0 68-69 0.0 0.0 0.0 1.0067626231109423 0.0 70-71 0.0 0.0 0.0 1.128771332447025 0.0 72-73 0.0 0.0 0.0 1.318355400602328 0.0 74-75 0.0 0.0 0.0 1.5455849635076104 0.0 76-77 0.0 0.0 0.0 1.7361864774669669 0.0 78-79 0.0 0.0 0.0 1.9228877825107848 0.0 80-81 1.69574300675584E-4 0.0 0.0 2.1851344385055755 0.0 82-83 1.69574300675584E-4 0.0 0.0 2.481719890387172 0.0 84-85 1.69574300675584E-4 0.0 0.0 2.793058306427544 0.0 86-87 1.69574300675584E-4 0.0 0.0 3.2050390699188753 0.0 88-89 1.69574300675584E-4 0.0 0.0 3.6832385978240225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3265 0.0 46.724247 1 ATACCGT 45 9.6246373E-4 42.219208 6 GTATCAA 5280 0.0 36.5437 1 ACAGCGC 80 4.488434E-4 29.690414 8 ACTTGAC 210 1.8189894E-12 29.40765 8 TATCAAC 6830 0.0 28.032217 2 ATCAACG 6835 0.0 28.01171 3 TCAACGC 6865 0.0 27.889297 4 CAACGCA 7070 0.0 27.013432 5 AACGCAG 7250 0.0 26.401564 6 CCAAGAC 285 0.0 25.004574 3 GTCCTAC 115 1.2761843E-4 24.795557 1 ATACGTC 115 1.2791282E-4 24.787144 5 CGGTTAC 135 1.8189894E-12 24.628914 28-29 GTACATG 4755 0.0 23.687449 1 ATGGGAT 1165 0.0 23.652397 5 GAGTACT 4875 0.0 23.386913 12-13 CGCAGAG 8200 0.0 23.346804 8 ACGCAGA 8205 0.0 23.332577 7 CATCCCG 125 2.251303E-4 22.804173 5 >>END_MODULE