FastQCFastQC Report
Thu 26 May 2016
SRR937802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553587
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72451.308737380032407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54300.9808756347240812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.6578911715773673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.2339289036772901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11030.19924600830583086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.1802787998995641No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.12482229532124128No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6360.1148870909179587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.10693892739533262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.1017003650736018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.051.982311
CGTAATA205.387278E-447.5014512-13
GTATCAA45800.042.350211
GTTAGGC702.0428728E-433.9541973
TATCAAC58500.032.7499052
ATCAACG59400.032.170753
TCAACGC59650.032.035914
CAACGCA60700.031.481755
AACGCAG61750.030.926826
TAGACTG1303.0120646E-729.2528485
TAGACCC856.3747197E-427.962285
ATGGGCG856.398402E-427.944567
ACGCAGA68900.027.5795357
CGCAGAG68900.027.4416378
GCAGAGT71300.025.9849119
GTACATG39650.025.8983121
TACATGG40650.024.7934072
GTGGTAT11700.024.3795831
GAGTACT45500.023.75072512-13
ACATGGG40000.023.64913
Produced by FastQC (version 0.11.5)