##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937802_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 553587 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7781486920755 33.0 31.0 34.0 30.0 34.0 2 31.877506155310726 33.0 31.0 34.0 30.0 34.0 3 31.88338237711507 33.0 31.0 34.0 30.0 34.0 4 35.5021161985379 37.0 35.0 37.0 33.0 37.0 5 35.49325038340857 37.0 35.0 37.0 33.0 37.0 6 35.59384342479141 37.0 35.0 37.0 33.0 37.0 7 35.547061256857546 37.0 35.0 37.0 33.0 37.0 8 35.53291352578727 37.0 35.0 37.0 33.0 37.0 9 37.137425553707004 39.0 37.0 39.0 34.0 39.0 10-11 37.02791882757363 39.0 37.0 39.0 33.0 39.0 12-13 36.88963252388514 39.0 37.0 39.0 33.0 39.0 14-15 37.95205541315096 40.0 38.0 41.0 33.0 41.0 16-17 37.926308782540055 40.0 38.0 41.0 33.0 41.0 18-19 37.96949621288073 40.0 38.0 41.0 33.0 41.0 20-21 37.80946806915625 40.0 38.0 41.0 33.0 41.0 22-23 37.702398177702875 40.0 37.5 41.0 32.5 41.0 24-25 37.56039881716876 40.0 37.0 41.0 32.0 41.0 26-27 37.60902983632202 40.0 37.0 41.0 32.0 41.0 28-29 37.52134443185985 40.0 37.0 41.0 32.0 41.0 30-31 37.21708602261252 39.0 36.5 41.0 32.0 41.0 32-33 36.87852406216186 39.0 35.5 41.0 31.0 41.0 34-35 36.5653763545748 39.0 35.0 40.5 30.5 41.0 36-37 36.53290630018407 39.0 35.0 41.0 30.0 41.0 38-39 36.445853136002114 39.0 35.0 41.0 30.0 41.0 40-41 36.19213691795508 39.0 35.0 41.0 30.0 41.0 42-43 35.865339142718305 39.0 35.0 41.0 29.5 41.0 44-45 35.473964345260995 39.0 35.0 41.0 27.5 41.0 46-47 34.87936313533374 38.5 35.0 40.0 24.0 41.0 48-49 34.37406405858519 38.0 34.0 40.0 21.5 41.0 50-51 33.39371769929568 37.5 33.0 39.5 18.5 40.5 52-53 33.4573436514947 38.0 33.5 39.5 18.0 40.5 54-55 33.730458807739346 38.0 34.0 40.0 16.0 41.0 56-57 33.46421700654098 38.0 33.5 40.0 11.5 41.0 58-59 33.22201659359776 38.0 33.0 40.0 8.5 41.0 60-61 32.91553089216329 37.0 33.0 40.0 7.5 41.0 62-63 32.5490175889246 37.0 32.5 40.0 4.5 41.0 64-65 32.174718698235324 36.0 32.0 39.0 2.0 41.0 66-67 31.75975320952262 35.5 31.5 39.0 2.0 41.0 68-69 31.290219062225088 35.0 31.0 39.0 2.0 40.0 70-71 30.853915464055333 35.0 31.0 38.0 2.0 40.0 72-73 30.404964350680203 35.0 30.0 37.5 2.0 39.5 74-75 29.93690512963635 35.0 29.5 37.0 2.0 39.0 76-77 29.49385101167477 34.5 29.0 36.0 2.0 39.0 78-79 28.983462400670536 34.0 29.0 36.0 2.0 38.5 80-81 28.47833041599604 34.0 28.0 35.0 2.0 37.0 82-83 27.987513254465874 34.0 27.0 35.0 2.0 37.0 84-85 27.532852108160057 34.0 26.0 35.0 2.0 36.0 86-87 27.04436249406146 33.0 25.5 35.0 2.0 36.0 88-89 26.57961982488751 33.0 25.0 35.0 2.0 35.5 90-91 26.058065850534785 33.0 23.5 35.0 2.0 35.0 92-93 25.524950007857846 32.0 20.5 35.0 2.0 35.0 94-95 24.92508223639645 32.0 18.0 34.5 2.0 35.0 96-97 24.177822094810754 31.5 4.5 34.0 2.0 35.0 98-99 23.32436094055677 31.0 2.0 34.0 2.0 35.0 100-101 20.940417675993114 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2885940322993221 1101 2 0.4581110897186029 1101 3 0.23720199659201313 1101 4 0.11001155631029036 1101 5 0.38116903920016654 1101 6 0.20054615689293342 1101 7 0.24966031709217873 1101 8 0.17530041154201115 1101 9 0.3663049939146461 1101 10-11 0.2605363080884189 1101 12-13 0.38203918512726176 1101 14-15 0.4340956362864503 1101 16-17 0.6486158389151839 1101 18-19 0.643668031030252 1101 20-21 0.5687912713017624 1101 22-23 0.6869682413074827 1101 24-25 0.491361659150904 1101 26-27 0.6272391527889383 1101 28-29 0.5489578825037569 1101 30-31 0.6362971207042918 1101 32-33 0.8054953321510538 1101 34-35 0.7200295118220197 1101 36-37 0.7928459014260199 1101 38-39 0.93607532331405 1101 40-41 1.0658825447637312 1101 42-43 1.1429760443248895 1101 44-45 1.2295468560869338 1101 46-47 1.6644072465054904 1101 48-49 1.6924305864067861 1101 50-51 1.3222780400009242 1101 52-53 1.1812138176331999 1101 54-55 1.251855338237192 1101 56-57 1.5313542662999566 1101 58-59 1.2439039823982014 1101 60-61 1.3818636852910444 1101 62-63 1.2227978438993077 1101 64-65 1.1873104916738022 1101 66-67 1.2769875489057547 1101 68-69 1.4362969619072636 1101 70-71 1.29597881057229 1101 72-73 1.2460379566508308 1101 74-75 1.1391658759004955 1101 76-77 1.0621220261509592 1101 78-79 1.4690638493421915 1101 80-81 1.5999228784909683 1101 82-83 1.7596451660052246 1101 84-85 1.7557782189085884 1101 86-87 1.8972548915139704 1101 88-89 2.0393180387860674 1101 90-91 1.9669708170373106 1101 92-93 2.1019668554454896 1101 94-95 2.248320375199061 1101 96-97 2.186045444804197 1101 98-99 2.6005371978870464 1101 100-101 2.4851684556258036 1102 1 0.23467909550483412 1102 2 0.13808825963362636 1102 3 0.23567999092676928 1102 4 0.23697219555267424 1102 5 0.16753863290921345 1102 6 0.19016692381467948 1102 7 0.08555322708245683 1102 8 0.08148038871192398 1102 9 0.10146015393662822 1102 10-11 0.11715069104195663 1102 12-13 0.12170751472604735 1102 14-15 0.14127337272580576 1102 16-17 0.2855197200296331 1102 18-19 0.17262577423407777 1102 20-21 0.1913761442565729 1102 22-23 0.30053470222154033 1102 24-25 0.24288894955803642 1102 26-27 0.46798081346622666 1102 28-29 0.342868802894408 1102 30-31 0.3647089500705434 1102 32-33 0.3072419393467598 1102 34-35 0.49608328935553203 1102 36-37 0.28496106652135467 1102 38-39 0.3121666225149937 1102 40-41 0.5406003395466357 1102 42-43 0.5568981683683418 1102 44-45 0.6001948711378802 1102 46-47 0.679851904688384 1102 48-49 0.6975330047046739 1102 50-51 0.5542940633383466 1102 52-53 0.7627309613358975 1102 54-55 0.7194017382710101 1102 56-57 0.769677629086921 1102 58-59 0.7913609645991997 1102 60-61 0.7489894198701705 1102 62-63 0.7173809834587743 1102 64-65 0.7049905956775255 1102 66-67 0.6608262797221407 1102 68-69 0.6047613216079419 1102 70-71 0.5588947197592837 1102 72-73 0.607384825969735 1102 74-75 0.5811209133164681 1102 76-77 0.5643072669237981 1102 78-79 0.6657249494140665 1102 80-81 0.7075730708555774 1102 82-83 0.776323811420184 1102 84-85 0.8893976329364044 1102 86-87 0.7837888406958946 1102 88-89 0.8418510155754788 1102 90-91 1.2917789809065887 1102 92-93 1.1727956086691798 1102 94-95 1.2875384447885416 1102 96-97 1.5982236040229019 1102 98-99 1.9081144608135254 1102 100-101 2.079207795244457 1103 1 0.1156898008315892 1103 2 0.3048157743254798 1103 3 0.10662427376409767 1103 4 0.10482500614258328 1103 5 0.09247474248276433 1103 6 0.07587077843893297 1103 7 0.07251751910586535 1103 8 0.09897959376191778 1103 9 -0.019741751587979195 1103 10-11 0.13982737604154494 1103 12-13 -0.05832657979645717 1103 14-15 0.08279596697154545 1103 16-17 0.09427167796205538 1103 18-19 0.017271197088597035 1103 20-21 0.3007980683888931 1103 22-23 0.059508068452061025 1103 24-25 0.2317243183559583 1103 26-27 -0.18735609812355847 1103 28-29 -0.014372897238679627 1103 30-31 0.05282945271448369 1103 32-33 0.07200666358743746 1103 34-35 0.2019032066807256 1103 36-37 0.03993392045076405 1103 38-39 0.24265634116157742 1103 40-41 0.46466347624269844 1103 42-43 0.2818822693682179 1103 44-45 0.2938115697988337 1103 46-47 0.39623361612742514 1103 48-49 0.5730504582737055 1103 50-51 0.3268353124109069 1103 52-53 0.32518606138183515 1103 54-55 0.5996277984987799 1103 56-57 0.5869384963479334 1103 58-59 0.6237045361034745 1103 60-61 0.7016987272626096 1103 62-63 0.7503625759497297 1103 64-65 0.6397759886183643 1103 66-67 0.7561415215636025 1103 68-69 0.7093450916730681 1103 70-71 0.9168459863796272 1103 72-73 0.8466772910213756 1103 74-75 0.6634769979227038 1103 76-77 0.5383440524992835 1103 78-79 0.567916656024515 1103 80-81 0.7613426060159192 1103 82-83 0.7617644492645113 1103 84-85 0.9852597709397273 1103 86-87 0.8047880339688511 1103 88-89 0.7795365039103253 1103 90-91 1.2445022735306317 1103 92-93 1.6259149041668195 1103 94-95 1.6027375944864595 1103 96-97 1.2578289555085753 1103 98-99 1.6661802591199866 1103 100-101 1.6381835168824068 1104 1 0.3435210446341017 1104 2 0.449122155145357 1104 3 0.3694766178563995 1104 4 0.3892273183346333 1104 5 0.23638717435842693 1104 6 0.4348793338569763 1104 7 0.5177278686250588 1104 8 0.4351102848828887 1104 9 0.41905203439589656 1104 10-11 0.4538190973486067 1104 12-13 0.437369768200746 1104 14-15 0.5906086471311269 1104 16-17 0.5789912631818552 1104 18-19 0.49612670814607185 1104 20-21 0.41055704850312225 1104 22-23 0.4968519301509957 1104 24-25 0.5361373152120521 1104 26-27 0.4665559109776254 1104 28-29 0.47333493485067635 1104 30-31 0.5648064614697645 1104 32-33 0.5432916289583645 1104 34-35 0.7994536129285663 1104 36-37 0.6555387185567341 1104 38-39 0.5033198664857679 1104 40-41 0.517685160729954 1104 42-43 0.6279990857995372 1104 44-45 0.7044668318037068 1104 46-47 0.4984085986399336 1104 48-49 0.7366440028917864 1104 50-51 0.7535675347441639 1104 52-53 0.6824900669778273 1104 54-55 0.6807130605805156 1104 56-57 0.8136916652348063 1104 58-59 0.9907237070042925 1104 60-61 0.9287468774321042 1104 62-63 1.184137836724716 1104 64-65 1.292321667478877 1104 66-67 1.378980567476905 1104 68-69 1.254341747911301 1104 70-71 1.351916474415109 1104 72-73 1.194805168770369 1104 74-75 1.1462921762950984 1104 76-77 1.0654506224146516 1104 78-79 1.324716599142132 1104 80-81 1.427694754094599 1104 82-83 1.4307695071846176 1104 84-85 1.344391315296459 1104 86-87 1.3158474299394278 1104 88-89 1.4099201525309262 1104 90-91 1.4877253908390173 1104 92-93 1.3345512258751349 1104 94-95 1.2828894858257307 1104 96-97 1.5730697832241347 1104 98-99 1.738458360170771 1104 100-101 1.1676307200370708 1105 1 0.1692647952281483 1105 2 0.2518642899234145 1105 3 0.2284206345848503 1105 4 0.19298646670456066 1105 5 0.1692642772954045 1105 6 0.22676223472191737 1105 7 0.1949496156072854 1105 8 0.2709481329961818 1105 9 0.18806638664378283 1105 10-11 0.3413606808482754 1105 12-13 0.47497313289991894 1105 14-15 0.4964612276842999 1105 16-17 0.5004079536105266 1105 18-19 0.4290750970824817 1105 20-21 0.494277702433358 1105 22-23 0.5519964031098397 1105 24-25 0.4040728726685714 1105 26-27 0.3073338788514022 1105 28-29 0.3782845594110782 1105 30-31 0.4463457638174546 1105 32-33 0.5172080817670306 1105 34-35 0.4834831728563316 1105 36-37 0.5874439557014952 1105 38-39 0.4795341046760555 1105 40-41 0.506345934420672 1105 42-43 0.45458638738683277 1105 44-45 0.639147470526261 1105 46-47 0.8948347938685188 1105 48-49 0.5412657118548125 1105 50-51 0.36804096882837456 1105 52-53 0.29242733529832776 1105 54-55 0.2857007043406128 1105 56-57 0.7374346554448579 1105 58-59 0.8684943561821328 1105 60-61 0.6220198552859273 1105 62-63 0.7264460773862496 1105 64-65 0.6066525295642329 1105 66-67 0.538751501798739 1105 68-69 0.50200356713 1105 70-71 0.5997422514325059 1105 72-73 0.8045761030922023 1105 74-75 0.6885386357571264 1105 76-77 0.5912822924079961 1105 78-79 0.6169773158040464 1105 80-81 0.8625060838161112 1105 82-83 1.293208914085099 1105 84-85 1.2879850801477062 1105 86-87 1.1394335909507376 1105 88-89 0.9363482487195007 1105 90-91 1.02475371514279 1105 92-93 1.4096985502739585 1105 94-95 1.4129389107853356 1105 96-97 1.4504079640054286 1105 98-99 1.4452376587165396 1105 100-101 1.443681000428672 1106 1 0.1985088092179268 1106 2 0.22703243627800518 1106 3 0.16670419659722313 1106 4 0.05720492244843456 1106 5 -0.003348044469454692 1106 6 0.1836360576974343 1106 7 0.1428646008104053 1106 8 0.05068986019553989 1106 9 0.2774933438673486 1106 10-11 -0.008827643068862301 1106 12-13 0.12879341807613542 1106 14-15 0.18641280153418194 1106 16-17 0.01979186536731703 1106 18-19 0.18597582347473463 1106 20-21 0.33396024211590003 1106 22-23 0.08311020456262952 1106 24-25 0.045356165646772695 1106 26-27 0.17069142525979686 1106 28-29 0.3289302414126922 1106 30-31 0.3860552069167227 1106 32-33 0.45744214149261353 1106 34-35 0.1650543323935878 1106 36-37 0.5475865438101835 1106 38-39 0.3670105641864083 1106 40-41 0.44631095997891634 1106 42-43 0.3038465434266513 1106 44-45 0.4128762840855842 1106 46-47 0.7559190015628943 1106 48-49 0.9337596585860481 1106 50-51 0.9895771539238822 1106 52-53 1.0032694122420978 1106 54-55 0.9702850465520783 1106 56-57 0.9279377493377865 1106 58-59 0.8835468178447599 1106 60-61 0.732835028812957 1106 62-63 0.7582593676741141 1106 64-65 0.6922322981948419 1106 66-67 0.8238873640532454 1106 68-69 0.8748042128120055 1106 70-71 0.9285020039210714 1106 72-73 0.8628758071546194 1106 74-75 1.0159666691173612 1106 76-77 0.8405289967394474 1106 78-79 0.7529068288522005 1106 80-81 0.6201601058769128 1106 82-83 0.36498722993121646 1106 84-85 0.4474281794213155 1106 86-87 0.7040286945288905 1106 88-89 1.1089740221927293 1106 90-91 1.0870620282985612 1106 92-93 0.7316651900372051 1106 94-95 0.6980478696231067 1106 96-97 0.6312796347076066 1106 98-99 0.573755280454499 1106 100-101 0.5041060744130696 1107 1 0.4983731052188105 1107 2 0.3916635429579607 1107 3 0.5426596635297614 1107 4 0.4418426758548861 1107 5 0.5425836050265005 1107 6 0.5506184438722386 1107 7 0.4743146949723638 1107 8 0.19312366987760043 1107 9 0.5860122316484464 1107 10-11 0.5269815958809545 1107 12-13 0.6910095474657467 1107 14-15 0.6074206114378029 1107 16-17 0.6296913336292036 1107 18-19 0.7542501671104915 1107 20-21 0.8572445558413833 1107 22-23 0.7471527990015332 1107 24-25 0.938281556141952 1107 26-27 0.6295094157328194 1107 28-29 0.518597351194451 1107 30-31 0.8112033735279951 1107 32-33 1.0931184459126868 1107 34-35 0.9634785043222465 1107 36-37 0.9415989510329581 1107 38-39 0.9068216863754017 1107 40-41 0.8264206309659556 1107 42-43 0.8865775171720927 1107 44-45 0.818804333271359 1107 46-47 0.9982544950823424 1107 48-49 1.1797577753468786 1107 50-51 1.0782417157938227 1107 52-53 1.038960948743707 1107 54-55 1.338735251492814 1107 56-57 1.158641471504616 1107 58-59 1.1642810041746614 1107 60-61 1.0635581372653817 1107 62-63 1.1203536404114587 1107 64-65 1.1175535566417274 1107 66-67 1.2185367492310455 1107 68-69 1.2975567884185217 1107 70-71 1.223166621180404 1107 72-73 1.2390182132696062 1107 74-75 1.3836483463080143 1107 76-77 1.560563338159632 1107 78-79 1.6794189591280393 1107 80-81 1.767419715935624 1107 82-83 1.3428937880346794 1107 84-85 1.0698594965809463 1107 86-87 1.149400911212176 1107 88-89 1.2415069788782311 1107 90-91 1.956523089559223 1107 92-93 1.3502330974261483 1107 94-95 1.1728595457059718 1107 96-97 1.7970116054620107 1107 98-99 2.147478555075079 1107 100-101 2.3019209630803985 1108 1 0.034016734867982734 1108 2 0.08102650787487065 1108 3 0.13746754183803134 1108 4 0.25491970038667233 1108 5 0.2435969700137477 1108 6 -0.0038306900414113443 1108 7 0.02364204923245694 1108 8 -0.04091748021472341 1108 9 0.30838099978350897 1108 10-11 0.29058838388763775 1108 12-13 0.17121485828962335 1108 14-15 0.30624128320426536 1108 16-17 0.31821349137121757 1108 18-19 0.13863093735580634 1108 20-21 0.371329449888691 1108 22-23 0.34524147393338467 1108 24-25 0.3528984118528484 1108 26-27 0.09726198761359228 1108 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-0.2426524308842346 2316 98-99 -0.05493655731133629 2316 100-101 0.3744640046872867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2106.0 3 456.0 4 449.0 5 458.0 6 517.0 7 568.0 8 762.0 9 987.0 10 1229.0 11 1683.0 12 2501.0 13 3400.0 14 3945.0 15 5465.0 16 7195.0 17 10143.0 18 8328.0 19 4814.0 20 4591.0 21 4450.0 22 4638.0 23 5215.0 24 5605.0 25 6276.0 26 7154.0 27 8280.0 28 9844.0 29 11452.0 30 13968.0 31 17385.0 32 21680.0 33 28263.0 34 38225.0 35 55700.0 36 75926.0 37 97949.0 38 73218.0 39 8762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.453886888678 18.697162437246906 15.409914445708159 20.439036228366938 2 15.324942882901802 20.51309438894662 41.29701977283507 22.864942955316504 3 18.7647656237271 26.73498907456991 30.569118054386372 23.931127247316617 4 12.065144975813226 17.01999391709875 37.65261564753932 33.26224545954871 5 12.683782594763112 38.08176322664393 33.532663107335736 15.701791071257226 6 29.829079162858875 36.7506039685484 17.77431934780445 15.64599752078828 7 27.12765764950692 34.99484302904189 20.452908712566725 17.424590608884465 8 26.949147021995756 33.589130230235305 19.605466007541818 19.856256740227124 9 27.647615549012762 16.341266121487294 20.654142862571728 35.35697546692822 10-11 24.74675956296514 27.440993507815502 26.27638156852987 21.53586536068948 12-13 26.49933495598042 25.27628733520933 27.771966811136544 20.452410897673705 14-15 23.226686766011237 25.475014409456588 27.786589840360413 23.51170898417177 16-17 20.555546005842615 29.304631118212075 28.191295426602263 21.948527449343043 18-19 20.413676549884737 26.129911300741515 33.10136179236919 20.355050357004565 20-21 21.702676614295964 24.953749313590055 33.43673584593297 19.90683822618101 22-23 21.313050527275752 25.01248525292589 33.59862987920065 20.0758343405977 24-25 20.78913036791222 25.205054111124582 33.78701875840256 20.21879676256064 26-27 20.29958934708992 25.61853520224588 33.70375631156062 20.378119139103585 28-29 20.227928476492842 25.57301048894422 34.026413718413515 20.17264731614943 30-31 20.32341214012415 25.403043761880507 33.860109275774306 20.413434822221035 32-33 20.007527681419838 25.74403711725992 33.61679758679905 20.631637614521185 34-35 20.70174454479161 25.60704404518698 33.45315279288213 20.23805861713928 36-37 20.703309468107626 25.404976053353888 33.257160020047515 20.63455445849097 38-39 20.33575406259159 25.66669561410174 33.42433683235455 20.573213490952117 40-41 20.44055907998397 25.7209901045629 33.32697096862027 20.511479846832852 42-43 20.44339097747762 25.771863083471676 32.810751394361034 20.973994544689667 44-45 20.415624033132676 25.98432747649212 32.7246705639901 20.8753779263851 46-47 20.79303403822232 26.39294357118625 31.905281013230802 20.908741377360624 48-49 21.071794041245674 26.624479407472286 31.142358475287068 21.161368075994975 50-51 21.13584875616129 26.957860756653467 30.80699329043652 21.09929719674872 52-53 21.13241146418663 27.233596285015736 30.46710570368078 21.16688654711685 54-55 20.833917607008026 27.115847220953153 30.5721841099876 21.47805106205122 56-57 20.987136149130574 27.453163769187 30.22077772883064 21.33892235285178 58-59 21.14260436285871 26.876704100334532 30.507172826305247 21.47351871050151 60-61 21.003453509681226 27.000511966076367 30.837815297835814 21.158219226406594 62-63 21.040329620131793 27.043595066552694 31.041188948137293 20.87488636517822 64-65 20.935022596273875 26.93515647331686 30.95336736946084 21.176453560948428 66-67 20.875278035825577 27.201243212488567 30.6776973432484 21.245781408437455 68-69 20.955931240541414 27.100609058352187 30.704935516523634 21.238524184582765 70-71 21.003050291822106 27.294865161180603 30.450904051496742 21.25118049550055 72-73 20.988130064532935 27.45245965108432 30.072764640220285 21.486645644162454 74-75 20.7293198936842 27.691322307320696 30.07239100187627 21.506966797118835 76-77 20.89213620767482 27.392784354734097 30.219302570244576 21.495776867346507 78-79 20.903680343907663 27.394014725717895 30.183469888629862 21.51883504174458 80-81 20.772861645119388 27.787939313019912 29.931088508479604 21.508110533381096 82-83 21.003266073268218 27.58380223026404 29.759254854112076 21.653676842355658 84-85 20.662327607415232 27.385819307969495 29.77353800958018 22.178315075035094 86-87 20.900822349974494 27.427976541499188 29.74985799719974 21.92134311132658 88-89 20.92215709685589 27.30353847823727 29.676182206302688 22.098122218604146 90-91 20.749064212482114 27.323186363282755 29.91028380277995 22.01746562145518 92-93 20.90398853054376 27.14814865066551 29.9125770547558 22.035285764034935 94-95 20.58142721847889 27.210699751519872 29.926432876444682 22.28144015355656 96-97 20.73914801504131 27.12898997638149 29.744845946002172 22.387016062575025 98-99 20.775803190599177 27.33656886203169 29.49418102375988 22.393446923609257 100-101 21.067142085711108 26.674481774246505 29.922545409483597 22.33583073055879 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1182.0 1 758.0 2 501.5 3 635.5 4 490.5 5 445.0 6 776.0 7 1714.0 8 2933.0 9 3346.0 10 2647.5 11 1967.0 12 1878.5 13 1899.5 14 1968.0 15 1993.0 16 1959.0 17 1963.5 18 1960.0 19 1968.0 20 2084.5 21 2332.0 22 2577.5 23 2969.0 24 3354.5 25 3826.5 26 4487.0 27 5141.0 28 5774.0 29 6512.0 30 7348.5 31 8268.0 32 9519.5 33 10470.5 34 11195.0 35 11969.0 36 13096.5 37 14160.0 38 14561.0 39 14847.5 40 15247.0 41 16168.0 42 16741.5 43 17274.5 44 18418.5 45 19477.0 46 19471.5 47 19509.0 48 20410.5 49 19739.0 50 18542.5 51 17626.0 52 16618.0 53 16260.5 54 15966.5 55 15082.5 56 13523.0 57 12008.0 58 11020.5 59 9860.5 60 8241.0 61 6875.5 62 5634.0 63 4267.0 64 3216.0 65 2473.0 66 1863.5 67 1438.5 68 1010.5 69 645.5 70 448.5 71 348.0 72 319.0 73 263.0 74 190.5 75 152.0 76 112.0 77 69.0 78 41.0 79 28.0 80 21.0 81 17.5 82 14.5 83 12.0 84 8.0 85 4.5 86 3.5 87 3.5 88 2.0 89 1.0 90 1.0 91 1.5 92 1.0 93 0.5 94 1.5 95 1.5 96 2.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25705083392492956 2 0.21911641711239607 3 0.21785193655197826 4 0.22020025759275416 5 0.22327113895376877 6 0.17919495941920602 7 0.1692597550159234 8 0.16889847485580406 9 0.17811111893884793 10-11 0.18054976001965364 12-13 0.17919495941920602 14-15 0.18036911993959395 16-17 0.1668211139351177 18-19 0.16853719469568468 20-21 0.1612212714532675 22-23 0.16889847485580406 24-25 0.16763399429538628 26-27 0.16808559449553548 28-29 0.17296287665714694 30-31 0.17133711593660977 32-33 0.17341447685729616 34-35 0.1716080760566993 36-37 0.16257607205371513 38-39 0.1555311089313875 40-41 0.15489886865117858 42-43 0.16519535321458054 44-45 0.16257607205371513 46-47 0.16248575201368531 48-49 0.1754918377779825 50-51 0.17034359549628153 52-53 0.18316904118051905 54-55 0.1637502325741031 56-57 0.15110542696992524 58-59 0.15824071013228275 60-61 0.14740230532870172 62-63 0.14984094640950746 64-65 0.1519183073301938 66-67 0.1491183860892687 68-69 0.15327310793064144 70-71 0.15408598829091 72-73 0.15282150773049225 74-75 0.16149223157335704 76-77 0.15489886865117858 78-79 0.1590535904925513 80-81 0.15336342797067126 82-83 0.14135086264670232 84-85 0.14071862236649343 86-87 0.14107990252661282 88-89 0.14577654460816458 90-91 0.15246022757037286 92-93 0.14713134520861218 94-95 0.1492990261693284 96-97 0.15218926745028333 98-99 0.15706654961189478 100-101 0.14658942496843316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 553587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.944523093586 #Duplication Level Percentage of deduplicated Percentage of total 1 80.71725166205239 47.5783990464464 2 11.035413136739201 13.009543289715722 3 3.426908346587637 6.059924345251128 4 1.4481380982798382 3.4143923830703056 5 0.8152932704565097 2.4028536504234497 6 0.5247593998342729 1.8559015537264574 7 0.3395284567094254 1.4009340070206835 8 0.255196565792924 1.2033951892627877 9 0.19589092739690273 1.039202756439364 >10 1.1373484139635481 12.21396906994619 >50 0.0719751727034765 2.89979569962988 >100 0.029220687628293985 2.8976198691075714 >500 0.0015379309278049204 0.6309421314287474 >1k 9.227585566829524E-4 1.0950834694912743 >5k 6.151723711219682E-4 2.29804353904005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 1.308737380032407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5430 0.9808756347240812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3642 0.6578911715773673 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.2339289036772901 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1103 0.19924600830583086 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.1802787998995641 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.12482229532124128 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 636 0.1148870909179587 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.10693892739533262 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.1017003650736018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.032004002984174E-5 0.0 22-23 0.0 0.0 0.0 3.6128016011936694E-4 0.0 24-25 0.0 0.0 0.0 9.032004002984174E-4 0.0 26-27 0.0 0.0 0.0 0.0016257607205371512 0.0 28-29 0.0 0.0 0.0 0.0032515214410743024 0.0 30-31 0.0 0.0 0.0 0.007677203402536548 0.0 32-33 0.0 0.0 0.0 0.013457685964446419 0.0 34-35 0.0 0.0 0.0 0.0175220877657893 0.0 36-37 0.0 0.0 0.0 0.021586489567132175 0.0 38-39 0.0 0.0 0.0 0.025650891368475055 0.0 40-41 0.0 0.0 0.0 0.03161201401044461 0.0 42-43 0.0 0.0 0.0 0.04642450057533866 0.0 44-45 0.0 0.0 0.0 0.06665618954202321 0.0 46-47 0.0 0.0 0.0 0.08896523942939412 0.0 48-49 0.0 0.0 0.0 0.13584134020488198 0.0 50-51 0.0 0.0 0.0 0.1776595187386987 0.0 52-53 0.0 0.0 0.0 0.22119377803308243 0.0 54-55 0.0 0.0 0.0 0.30997837738241685 0.0 56-57 0.0 0.0 0.0 0.39081481320912526 0.0 58-59 0.0 0.0 0.0 0.48610245544060826 0.0 60-61 0.0 0.0 0.0 0.5880737806342995 0.0 62-63 0.0 0.0 0.0 0.7195797589177492 0.0 64-65 0.0 0.0 0.0 0.8296798877141263 0.0 66-67 0.0 0.0 0.0 0.9802433944438724 0.0 68-69 0.0 0.0 0.0 1.1612447546636753 0.0 70-71 0.0 0.0 0.0 1.293292653187304 0.0 72-73 0.0 0.0 0.0 1.4958805029742388 0.0 74-75 0.0 0.0 0.0 1.7454347735766915 0.0 76-77 0.0 0.0 0.0 1.9565127071264319 0.0 78-79 0.0 0.0 0.0 2.155578075352203 0.0 80-81 0.0 0.0 0.0 2.433041238323877 0.0 82-83 1.8064008005968347E-4 0.0 0.0 2.7467227373475174 0.0 84-85 1.8064008005968347E-4 0.0 0.0 3.0825326461784686 0.0 86-87 1.8064008005968347E-4 0.0 0.0 3.517875239122306 0.0 88-89 1.8064008005968347E-4 0.0 0.0 4.038750909974404 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2515 0.0 51.98231 1 CGTAATA 20 5.387278E-4 47.50145 12-13 GTATCAA 4580 0.0 42.35021 1 GTTAGGC 70 2.0428728E-4 33.954197 3 TATCAAC 5850 0.0 32.749905 2 ATCAACG 5940 0.0 32.17075 3 TCAACGC 5965 0.0 32.03591 4 CAACGCA 6070 0.0 31.48175 5 AACGCAG 6175 0.0 30.92682 6 TAGACTG 130 3.0120646E-7 29.252848 5 TAGACCC 85 6.3747197E-4 27.96228 5 ATGGGCG 85 6.398402E-4 27.94456 7 ACGCAGA 6890 0.0 27.579535 7 CGCAGAG 6890 0.0 27.441637 8 GCAGAGT 7130 0.0 25.984911 9 GTACATG 3965 0.0 25.898312 1 TACATGG 4065 0.0 24.793407 2 GTGGTAT 1170 0.0 24.379583 1 GAGTACT 4550 0.0 23.750725 12-13 ACATGGG 4000 0.0 23.6491 3 >>END_MODULE