FastQCFastQC Report
Thu 26 May 2016
SRR937802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553587
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62541.1297230606932605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52710.9521538619945917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.6721617379020822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16710.3018495737797311No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.24241898744009524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.18009815981950444No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.11452581075783934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.046.42461
GTATCAA50950.035.3481831
ATCGCGT300.00394993331.66823224-25
GTCCGTG804.4893645E-429.6889677
GTCTAGA1004.934691E-528.5117111
TGTCCGT856.4044824E-427.9400336
AGGTAAG1554.5722118E-827.58452
TATCAAC65600.027.3018322
TCCGACC700.00838585527.14428
CGGTTAC700.00838585527.14429
ATCAACG67300.026.7533663
TCAACGC67900.026.4469954
CCTTATA1806.672053E-926.3925762
CCCACGT908.939085E-426.3901927
CAACGCA68950.026.0442495
AACGCAG70250.025.5576746
GTATGAC1151.2793654E-424.7860743
CATGGGT4050.024.6330744
GTACATG42650.024.5118271
TAAGGTG2351.7462298E-1024.2587095
Produced by FastQC (version 0.11.5)