##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 553587 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21987691184945 33.0 31.0 34.0 31.0 34.0 2 32.42210709427786 34.0 31.0 34.0 31.0 34.0 3 32.47139293372135 34.0 31.0 34.0 31.0 34.0 4 36.11102500600628 37.0 35.0 37.0 35.0 37.0 5 36.04083549649829 37.0 35.0 37.0 35.0 37.0 6 36.09764318887546 37.0 35.0 37.0 35.0 37.0 7 36.042576866870064 37.0 35.0 37.0 35.0 37.0 8 36.02708156080255 37.0 35.0 37.0 35.0 37.0 9 37.691734090576546 39.0 37.0 39.0 35.0 39.0 10-11 37.625929619012005 39.0 37.0 39.0 35.0 39.0 12-13 37.51779214468547 39.0 37.0 39.0 35.0 39.0 14-15 38.664593821747985 40.0 38.0 41.0 34.0 41.0 16-17 38.64705005717259 40.0 38.0 41.0 34.5 41.0 18-19 38.69463426706191 40.0 38.0 41.0 34.5 41.0 20-21 38.6265221184746 40.0 38.0 41.0 34.0 41.0 22-23 38.468166701891484 40.0 38.0 41.0 34.0 41.0 24-25 38.49825411362622 40.0 38.0 41.0 34.0 41.0 26-27 38.343627108295536 40.0 38.0 41.0 34.0 41.0 28-29 38.17490204791659 40.0 38.0 41.0 34.0 41.0 30-31 38.12205940529673 40.0 37.0 41.0 34.0 41.0 32-33 37.94288341308592 40.0 37.0 41.0 33.5 41.0 34-35 37.76354484480308 40.0 36.0 41.0 33.0 41.0 36-37 37.55730716219854 40.0 36.0 41.0 33.0 41.0 38-39 37.35541658312063 40.0 36.0 41.0 33.0 41.0 40-41 37.121562645076565 40.0 35.5 41.0 32.5 41.0 42-43 36.82908738825153 39.5 35.0 41.0 31.5 41.0 44-45 36.36556584601878 39.0 35.0 41.0 30.5 41.0 46-47 35.811889549429445 39.0 35.0 41.0 29.0 41.0 48-49 35.29256377046426 39.0 35.0 41.0 26.5 41.0 50-51 34.94561920709843 39.0 35.0 40.0 24.5 41.0 52-53 34.65725080249355 38.5 35.0 40.0 24.0 41.0 54-55 34.40679965389361 38.0 34.0 40.0 23.0 41.0 56-57 34.12049596540381 38.0 34.0 40.0 22.5 41.0 58-59 33.83247709935385 38.0 34.0 40.0 21.5 41.0 60-61 33.458865544169214 37.0 33.0 40.0 19.5 41.0 62-63 33.08695832813993 36.5 33.0 40.0 18.0 41.0 64-65 32.69045244920852 36.0 32.5 39.0 15.5 41.0 66-67 32.24813985877559 35.5 32.0 39.0 11.5 40.0 68-69 31.81008495502965 35.0 31.5 38.5 7.5 40.0 70-71 31.375139770261946 35.0 31.0 38.0 6.5 40.0 72-73 30.898744009523348 35.0 31.0 37.0 2.0 39.5 74-75 30.403102854655184 34.5 30.0 36.5 2.0 39.0 76-77 29.193117793589806 33.5 28.5 35.0 2.0 37.5 78-79 29.780397661072243 34.0 30.0 36.0 2.0 38.0 80-81 29.589778119789663 34.0 30.0 35.0 2.0 37.0 82-83 29.228134873109376 34.0 29.5 35.0 2.0 37.0 84-85 28.84495120008237 34.0 29.0 35.0 2.0 36.0 86-87 28.412550330842308 34.0 29.0 35.0 2.0 36.0 88-89 28.0535706221425 34.0 28.5 35.0 2.0 35.5 90-91 27.526336420472305 33.0 27.0 35.0 2.0 35.0 92-93 27.03807441287458 33.0 26.0 35.0 2.0 35.0 94-95 26.543572193711196 33.0 25.0 35.0 2.0 35.0 96-97 25.985582212010037 33.0 24.5 35.0 2.0 35.0 98-99 25.13202351211282 32.0 19.0 34.0 2.0 35.0 100-101 22.623237178618716 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2602754256054638 1101 2 0.24379153679025478 1101 3 0.1936597563888256 1101 4 0.10427194730787193 1101 5 0.1419758839300158 1101 6 0.09295226438520388 1101 7 0.09445014676088448 1101 8 0.09175369950951051 1101 9 0.10972151431069932 1101 10-11 0.308984522144641 1101 12-13 0.2715082592730411 1101 14-15 0.2895597464554527 1101 16-17 0.23855015110829925 1101 18-19 0.2898680561034368 1101 20-21 0.26413714853983805 1101 22-23 0.16649504364270484 1101 24-25 0.11454686822419546 1101 26-27 0.18394521761685212 1101 28-29 0.22898444514851946 1101 30-31 0.1933818778130032 1101 32-33 0.36873847257667336 1101 34-35 0.14577653731445395 1101 36-37 0.26821297381513176 1101 38-39 0.23261173593365925 1101 40-41 0.20117532812140126 1101 42-43 0.27146268771358706 1101 44-45 0.2584128754260533 1101 46-47 0.005035765984409579 1101 48-49 0.15627992698750148 1101 50-51 0.22093840018087008 1101 52-53 0.1375808331600794 1101 54-55 0.2040911334758917 1101 56-57 0.19123118247838278 1101 58-59 0.05053567891562949 1101 60-61 0.1971437856589091 1101 62-63 0.21896964279910947 1101 64-65 0.34767024463737783 1101 66-67 0.11645566131300455 1101 68-69 0.06955819846304578 1101 70-71 0.020487714553940606 1101 72-73 0.04859602200538049 1101 74-75 0.14786115378489484 1101 76-77 -0.0694282250202427 1101 78-79 0.1211130052047018 1101 80-81 -0.054986767086756316 1101 82-83 -0.031038274367737984 1101 84-85 -0.00955592344813283 1101 86-87 -0.0278116625706879 1101 88-89 0.28498082006751346 1101 90-91 0.3261023073978073 1101 92-93 0.5055911754320661 1101 94-95 0.23035010515173937 1101 96-97 0.48423036150780163 1101 98-99 0.6509357012875547 1101 100-101 1.04693662698514 1102 1 0.08627001402976475 1102 2 0.19529874517819934 1102 3 0.22646743406185266 1102 4 0.1023060233235995 1102 5 0.07361774754139105 1102 6 0.12198663634647744 1102 7 0.06188028247306221 1102 8 0.11550332957757803 1102 9 0.04431754864038595 1102 10-11 0.20058653880214905 1102 12-13 0.14486206217330988 1102 14-15 0.36517647583439583 1102 16-17 0.3161750824066516 1102 18-19 0.26204124766476156 1102 20-21 0.20981000187767762 1102 22-23 0.18908467286521358 1102 24-25 0.07979440553447148 1102 26-27 0.1553045589266162 1102 28-29 0.157899381943011 1102 30-31 0.21395802037419998 1102 32-33 0.3617969640999519 1102 34-35 0.2564786681223836 1102 36-37 0.19921274128648037 1102 38-39 0.12348181583896434 1102 40-41 0.09381051181902222 1102 42-43 0.07353853432923074 1102 44-45 0.23303818722583003 1102 46-47 0.247724325575156 1102 48-49 0.08286165023613279 1102 50-51 -0.04566361647663797 1102 52-53 0.14382739807785327 1102 54-55 0.12743595231493998 1102 56-57 -0.22746570586559045 1102 58-59 -0.03597554587615548 1102 60-61 0.05204591223905197 1102 62-63 0.09502659117774925 1102 64-65 0.0765048533550825 1102 66-67 0.2852846682043051 1102 68-69 0.08075920890569321 1102 70-71 0.10157415179234519 1102 72-73 0.176957117849458 1102 74-75 -0.12629804377989373 1102 76-77 0.029938134426760854 1102 78-79 0.061495831907446785 1102 80-81 -0.15758738857240218 1102 82-83 -0.09888643316366341 1102 84-85 0.07218582178503397 1102 86-87 0.1735475730442495 1102 88-89 0.023232838838918468 1102 90-91 0.08203971491482775 1102 92-93 0.15664231059461997 1102 94-95 0.1378539101659051 1102 96-97 0.3001733708512262 1102 98-99 0.1853816489474731 1102 100-101 0.7321404066325421 1103 1 0.21477221044718675 1103 2 0.31934627730007037 1103 3 0.041176364589105674 1103 4 0.08744634672103757 1103 5 0.10030009729584322 1103 6 0.1470923352547402 1103 7 0.10975246628905211 1103 8 0.06538614805810994 1103 9 0.19632965543455327 1103 10-11 0.1162857709393208 1103 12-13 0.20708530180068152 1103 14-15 0.13584885632690913 1103 16-17 0.20860288916902903 1103 18-19 0.1656969872038161 1103 20-21 0.08513239826918806 1103 22-23 0.15971200619069492 1103 24-25 0.14505685680698832 1103 26-27 0.024042855495963522 1103 28-29 0.07310177032613296 1103 30-31 0.14720945422846654 1103 32-33 0.23771042054257663 1103 34-35 0.16395016769251924 1103 36-37 0.15908911183918661 1103 38-39 -0.005400190026001894 1103 40-41 -0.3540657939599683 1103 42-43 -0.13739556137096542 1103 44-45 -0.13142062847531832 1103 46-47 -0.1310597836686398 1103 48-49 0.1182008583481533 1103 50-51 0.010556278472186875 1103 52-53 -0.11668213543370598 1103 54-55 0.11890348891208191 1103 56-57 -0.013445290371720375 1103 58-59 -0.3262412358338693 1103 60-61 -0.5189166250058292 1103 62-63 -0.40876708690871766 1103 64-65 -0.2864428212689347 1103 66-67 -0.30009811494511496 1103 68-69 -0.19369212837687044 1103 70-71 -0.19419656696023324 1103 72-73 -0.2833513227530524 1103 74-75 -0.4631956483305437 1103 76-77 -0.3366376036990424 1103 78-79 -0.12334476589495935 1103 80-81 -0.11560004299880333 1103 82-83 -0.11028734117322614 1103 84-85 -0.07480411473673598 1103 86-87 0.0010434216386592254 1103 88-89 -0.004256237959140208 1103 90-91 0.01920554148617626 1103 92-93 0.17671553432299802 1103 94-95 0.04130796794404645 1103 96-97 0.011122187847846021 1103 98-99 0.2062049227282543 1103 100-101 0.13461476213418422 1104 1 0.20583821707321448 1104 2 0.16787194739761446 1104 3 -0.006062918077908819 1104 4 -0.012922235754579958 1104 5 0.13511173204944527 1104 6 0.15897229805059254 1104 7 0.10649518111613787 1104 8 0.19581976093505205 1104 9 0.258842626924185 1104 10-11 0.2238828361157772 1104 12-13 0.2704217226688286 1104 14-15 0.3421205235188438 1104 16-17 0.2094378687605527 1104 18-19 0.19443219970563774 1104 20-21 0.10569941571056063 1104 22-23 0.10600427402985702 1104 24-25 -0.08948401011129903 1104 26-27 0.09147922793722074 1104 28-29 0.06477599568799519 1104 30-31 0.13032826154323374 1104 32-33 -0.04945049511007227 1104 34-35 0.17105662230576257 1104 36-37 -0.02308921443509604 1104 38-39 -0.0232797069869477 1104 40-41 0.11525615264746136 1104 42-43 0.13522479483575012 1104 44-45 -0.20590388276263383 1104 46-47 -0.4234390815555287 1104 48-49 -0.27480260840254545 1104 50-51 -0.14455871618547889 1104 52-53 -0.11294346386797827 1104 54-55 -0.37949285062962446 1104 56-57 -0.3781036501604902 1104 58-59 -0.37627659515374745 1104 60-61 -0.3204201647812255 1104 62-63 -0.28938806574692677 1104 64-65 -0.27387008484191 1104 66-67 -0.11400047710142047 1104 68-69 -0.040268231626086504 1104 70-71 -0.2525192668749092 1104 72-73 -0.1632958624285159 1104 74-75 -0.5597121788125818 1104 76-77 -0.3259750392259342 1104 78-79 0.0010662323908796623 1104 80-81 -0.11803003538886259 1104 82-83 -0.1041225206217753 1104 84-85 0.13424633378854622 1104 86-87 0.1604178691492848 1104 88-89 0.14083707989808403 1104 90-91 0.11521762514940193 1104 92-93 0.30375061691775684 1104 94-95 0.3289041491375002 1104 96-97 0.42268064987116816 1104 98-99 0.5950325306702631 1104 100-101 0.7042947218553053 1105 1 0.13661951162696795 1105 2 0.04537883837755885 1105 3 0.213193806568043 1105 4 0.09395448699041253 1105 5 0.14704500835397738 1105 6 0.027207256704713245 1105 7 0.13072766852228312 1105 8 0.16837940355457448 1105 9 0.08181572834039486 1105 10-11 0.08060510586199854 1105 12-13 0.2685896724834862 1105 14-15 0.2966257986827969 1105 16-17 0.22110263446723621 1105 18-19 0.3081476259959146 1105 20-21 0.08589854126896768 1105 22-23 0.16166913478453182 1105 24-25 0.19406555742030918 1105 26-27 0.11647209933727254 1105 28-29 0.2082343171403238 1105 30-31 0.34802857520163144 1105 32-33 0.24755311671390245 1105 34-35 0.21614264074507616 1105 36-37 0.1745749809835857 1105 38-39 0.22403518703453074 1105 40-41 0.38255013610910993 1105 42-43 0.28947344040175693 1105 44-45 0.06800068904612999 1105 46-47 0.383760804386867 1105 48-49 0.44434956344422716 1105 50-51 0.4334448005904932 1105 52-53 0.6235383344401626 1105 54-55 0.5412775134042036 1105 56-57 0.5414999996826779 1105 58-59 0.5172791504978065 1105 60-61 0.6253311896527691 1105 62-63 0.8581145480730541 1105 64-65 0.7641705006537549 1105 66-67 0.7437982112361965 1105 68-69 0.7304260940083545 1105 70-71 0.8155850007802563 1105 72-73 0.7507593604590426 1105 74-75 0.681949735454122 1105 76-77 0.586882578871208 1105 78-79 0.9273470041806355 1105 80-81 0.7110142569787818 1105 82-83 0.8054440605016921 1105 84-85 1.0621286643654884 1105 86-87 1.0280255110084227 1105 88-89 0.7199526582651607 1105 90-91 0.7018063524566927 1105 92-93 0.9291830853142997 1105 94-95 0.8919732490329473 1105 96-97 1.0975048110725751 1105 98-99 1.1303903863874005 1105 100-101 1.4360431298013197 1106 1 0.11334805615211963 1106 2 0.2505173909737408 1106 3 0.11550750040715485 1106 4 0.1347400667590435 1106 5 0.12172889320735436 1106 6 0.005307875483296698 1106 7 0.1957263233514439 1106 8 0.0832031861749627 1106 9 -0.06830533703490715 1106 10-11 -0.07083422693326469 1106 12-13 -0.17799359359130307 1106 14-15 0.010117862174865877 1106 16-17 -0.14288714223440735 1106 18-19 0.11321892005026513 1106 20-21 0.14304139841183172 1106 22-23 0.10986660586335972 1106 24-25 0.3153461600568406 1106 26-27 0.1827486664613076 1106 28-29 0.42806213527322967 1106 30-31 0.2623142894873425 1106 32-33 0.2542655191890191 1106 34-35 0.2129276824453683 1106 36-37 0.030776218540758293 1106 38-39 0.07929480500601471 1106 40-41 0.24173765292374583 1106 42-43 0.05408199568827854 1106 44-45 -0.2619697171954627 1106 46-47 -0.09898065377832665 1106 48-49 -0.24085355735211067 1106 50-51 -0.21186055318943176 1106 52-53 -0.29327630101853686 1106 54-55 -0.267802389743494 1106 56-57 -0.3455191017432 1106 58-59 -0.24393957428024038 1106 60-61 -0.03682915471096493 1106 62-63 -0.1202954599979833 1106 64-65 -0.15268448993004569 1106 66-67 -0.07387733355799497 1106 68-69 -0.2866441239093156 1106 70-71 -0.030540907210056645 1106 72-73 -0.2523479841737277 1106 74-75 -0.18146161778773973 1106 76-77 -0.2884469879837859 1106 78-79 -0.20052493555570905 1106 80-81 -0.014907185044354065 1106 82-83 -0.11501598792445833 1106 84-85 0.03354378408569403 1106 86-87 -0.13479396706528846 1106 88-89 -0.3085649634728007 1106 90-91 -0.18627543393017731 1106 92-93 -0.05207868493052459 1106 94-95 0.5080345888231008 1106 96-97 0.6439401083553271 1106 98-99 0.5798792214694579 1106 100-101 0.025174149440815086 1107 1 0.1432721726913897 1107 2 0.18951982810678203 1107 3 0.017302116558703062 1107 4 0.05315149912859596 1107 5 0.04627470023072533 1107 6 0.062151234295747315 1107 7 0.20500404573983388 1107 8 0.20789854454429957 1107 9 0.067343272691474 1107 10-11 0.18802807504967234 1107 12-13 0.31337258564875725 1107 14-15 0.2936145942010029 1107 16-17 0.3360302721889852 1107 18-19 0.18440813019759617 1107 20-21 0.2884662350131322 1107 22-23 0.3557400965025579 1107 24-25 0.36816686460984727 1107 26-27 0.24665417216641572 1107 28-29 0.38562694085648275 1107 30-31 0.4730986032128399 1107 32-33 0.3898033501406033 1107 34-35 0.39526262207093055 1107 36-37 0.14895640021794776 1107 38-39 0.5795440572492865 1107 40-41 0.8103512565572899 1107 42-43 0.6754678381608556 1107 44-45 0.7144759458155008 1107 46-47 0.49570058029723185 1107 48-49 0.3961936344059538 1107 50-51 0.5820909257072131 1107 52-53 0.29099865190048746 1107 54-55 0.3890949737216687 1107 56-57 0.19962324898239814 1107 58-59 0.5575102429347822 1107 60-61 0.7851701252269976 1107 62-63 0.6871294873821014 1107 64-65 0.6919482784315392 1107 66-67 0.6626007322445915 1107 68-69 0.5248588671176044 1107 70-71 0.7880173070360961 1107 72-73 0.8658737395440106 1107 74-75 0.5686560846604714 1107 76-77 0.5434185265836255 1107 78-79 0.7066770695401132 1107 80-81 0.42734245492463074 1107 82-83 0.3177689904736809 1107 84-85 0.6385759099303812 1107 86-87 0.4996291524650083 1107 88-89 0.5670581671353752 1107 90-91 0.6425159696369 1107 92-93 0.8121896212620072 1107 94-95 0.9276992060824334 1107 96-97 1.136767182687887 1107 98-99 1.3452013107571474 1107 100-101 1.736311570510935 1108 1 -0.0025007104528995683 1108 2 0.07595040930787178 1108 3 0.12263931870413813 1108 4 -0.004792754806160815 1108 5 -0.054940898116235815 1108 6 -0.06858603819172515 1108 7 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84-85 -0.7828432674966876 2316 86-87 -0.6406446399787988 2316 88-89 -0.7333961609373176 2316 90-91 -0.31704285389251297 2316 92-93 -0.1628223347399036 2316 94-95 0.026545451897835193 2316 96-97 -0.23013262601608275 2316 98-99 -0.7742989283628923 2316 100-101 -0.24174916597878138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 18.0 6 34.0 7 93.0 8 178.0 9 343.0 10 634.0 11 1107.0 12 1648.0 13 2418.0 14 3194.0 15 4512.0 16 6528.0 17 9650.0 18 8534.0 19 4800.0 20 3723.0 21 3595.0 22 3670.0 23 3878.0 24 4361.0 25 4943.0 26 5719.0 27 6852.0 28 8400.0 29 10252.0 30 12954.0 31 16166.0 32 20416.0 33 27056.0 34 37692.0 35 54882.0 36 80759.0 37 113978.0 38 83659.0 39 6934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33860674871898 19.989412215340362 15.567476349129489 20.10450468681116 2 15.842315661314302 21.651339355873603 39.63875596789664 22.867589014915453 3 19.269780540366735 26.76056338028169 30.507580560959706 23.46207551839187 4 12.588807179359343 17.858258954780368 36.55324276039719 32.9996911054631 5 12.790174578999572 39.11144113040038 32.83234237212807 15.266041918471988 6 30.222711154705586 37.16308367067868 16.942233108797712 15.671972065818018 7 27.38792637832897 35.38395952217086 19.805017097583576 17.42309700191659 8 27.61173943752292 34.056796853972365 18.89766197544379 19.43380173306093 9 27.806469443827257 16.644176976699235 20.017269191653707 35.5320843878198 10-11 25.061282147160245 27.984761202846165 25.58549965949345 21.368456990500135 12-13 26.542250900511945 25.71923536871313 27.207202650413493 20.531311080361434 14-15 23.24015918003855 26.265790200998218 27.06449031498211 23.429560303981127 16-17 20.783815371386975 29.87714668155141 27.53289004257687 21.806147904484753 18-19 20.719417182845696 26.677468943454237 32.3380968122445 20.265017061455563 20-21 21.781309893476543 25.53347531643626 32.75943979898372 19.925774991103477 22-23 21.443867896835613 25.753342997656176 32.800079482281326 20.00270962322689 24-25 21.075026215415296 26.01102086123849 32.90452350265045 20.00942942069577 26-27 20.674116912805385 26.314729394933195 32.77653687293787 20.234616819323552 28-29 20.379205136032976 26.47670883469207 33.02175479922468 20.12233123005027 30-31 20.468959711842942 26.330639989739645 32.83277967148795 20.36762062692946 32-33 20.418561129506294 26.58109746074239 32.71726034028978 20.28308106946153 34-35 20.811453303636103 26.374806489314235 32.769194363306944 20.044545843742718 36-37 21.15241145474876 26.130129500873394 32.29230455194938 20.425154492428472 38-39 20.83795471706293 26.522347024671866 32.34348411990188 20.296214138363325 40-41 20.745127650234924 26.57361226771776 32.38764557437638 20.293614507670938 42-43 20.832302041686464 26.741016019497284 31.78642597373503 20.640255965081217 44-45 20.626387541614957 27.02610429796942 31.488636835763845 20.85887132465177 46-47 21.05974309367814 27.487007462241706 30.695807524381895 20.75744191969826 48-49 21.36719250993972 27.682821309026405 29.970447282902235 20.97953889813164 50-51 21.56634819820552 27.939510862791217 29.60718008190221 20.88696085710105 52-53 21.5402457066369 28.34938320444664 29.231629355458132 20.878741733458337 54-55 21.074103980042885 28.300249102670406 29.345432605895734 21.28021431139098 56-57 21.380740515944197 28.614201561814134 29.000951973221916 21.00410594901976 58-59 21.33278057468835 28.088990529040604 29.194417498965837 21.383811397305212 60-61 21.212654921448664 28.31768087039616 29.648907940395997 20.820756267759176 62-63 21.243002454898686 28.317229270196016 29.779781678399242 20.659986596506062 64-65 20.96743601276764 28.212728983881487 29.707615966415396 21.11221903693548 66-67 21.006273629980473 28.54989369331288 29.33098139949096 21.112851277215686 68-69 21.13142414315404 28.447276888574574 29.39004002091818 21.03125894735321 70-71 21.13705704794368 28.68808335455854 29.07058872408492 21.10427087341285 72-73 21.085935905286792 28.74101089801603 28.738572256935225 21.43448093976195 74-75 21.14283753050559 29.062911520682388 28.43780652363585 21.356444425176168 76-77 21.03607924319032 28.726017771371076 28.917225296114253 21.320677689324352 78-79 21.04827244859435 28.793215881153277 28.689167195038902 21.46934447521347 80-81 21.16615921811677 29.24773273914736 28.353292574873123 21.232815467862746 82-83 21.062937719819573 29.169368780246362 28.24411691917504 21.523576580759023 84-85 20.893972114515282 29.150600946014666 28.23972087419197 21.71570606527808 86-87 21.011963792502353 29.079530408047876 27.934633580629605 21.97387221882017 88-89 20.794021535910346 29.092897773972293 28.122047663691525 21.99103302642584 90-91 20.86136953040104 29.162703434620447 28.098705479693347 21.877221555285168 92-93 20.91195167376904 29.01028052408815 28.092241103670535 21.98552669847228 94-95 20.669470200709192 28.794119081553575 28.17488488710898 22.36152583062825 96-97 20.922005032632633 28.79908668375522 28.035430745302907 22.243477538309246 98-99 20.74134422495215 29.044999281940765 27.601564736878707 22.612091756228374 100-101 20.582889478286635 28.940402974611736 27.901709895859682 22.57499765124195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1200.0 1 783.0 2 441.5 3 546.5 4 505.5 5 484.5 6 781.5 7 1532.0 8 2319.0 9 2634.0 10 2365.0 11 1986.5 12 1979.5 13 2054.5 14 2120.5 15 2172.5 16 2143.0 17 2135.0 18 2163.0 19 2169.5 20 2201.5 21 2373.0 22 2604.5 23 2946.0 24 3369.0 25 3843.5 26 4380.5 27 4948.0 28 5569.5 29 6411.0 30 7517.0 31 8407.0 32 9293.0 33 10079.0 34 11047.5 35 11965.0 36 13023.5 37 14164.0 38 14666.5 39 14938.5 40 15141.5 41 15972.5 42 16421.0 43 17132.0 44 18528.5 45 19603.0 46 19294.5 47 18861.5 48 20208.5 49 19920.0 50 18802.5 51 17857.0 52 16511.0 53 16063.5 54 15973.5 55 15170.0 56 13449.5 57 12214.0 58 11679.5 59 10549.0 60 8689.0 61 7132.0 62 5883.5 63 4402.0 64 3078.0 65 2326.5 66 1772.0 67 1435.0 68 1060.0 69 646.5 70 463.5 71 342.0 72 295.0 73 275.5 74 221.5 75 166.0 76 134.0 77 85.0 78 51.0 79 35.5 80 24.0 81 20.0 82 14.5 83 7.5 84 4.0 85 3.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021496169527102336 2 0.0 3 0.0 4 0.0 5 0.015173766725013412 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0023483210407758853 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.128803602685757E-4 24-25 9.032004002984174E-5 26-27 6.322402802088922E-4 28-29 0.0014451206404774678 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.8064008005968347E-4 40-41 7.225603202387339E-4 42-43 0.013367365924416577 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.7096012008952523E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.032004002984174E-5 82-83 0.0014451206404774678 84-85 2.7096012008952523E-4 86-87 0.0 88-89 0.0 90-91 0.011109364923670534 92-93 0.0028902412809549355 94-95 0.0 96-97 0.0 98-99 0.00207736092068636 100-101 0.0186059282461474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 553587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.541271541753815 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22460819128491 44.794600845324936 2 11.653936459160192 13.178567717354428 3 3.7139868918389585 6.299806240619424 4 1.7284889241070758 3.909238464594083 5 0.8951539070166027 2.530657006414379 6 0.5573193902963884 1.8906928189339665 7 0.3944291535158909 1.5611068111048263 8 0.27909405870422793 1.2624266367108745 9 0.2094623315791333 1.0658939910826186 >10 1.2167557521849635 12.630040934423064 >50 0.08643064237287043 3.3301497465406387 >100 0.037133163181742375 3.65757180654283 >500 0.001600567378523367 0.5844368251844194 >1k 9.603404271140201E-4 1.2188286097218581 >5k 6.402269514093467E-4 2.085981545447641 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6254 1.1297230606932605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5271 0.9521538619945917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3721 0.6721617379020822 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1671 0.3018495737797311 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1342 0.24241898744009524 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 997 0.18009815981950444 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.11452581075783934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.032004002984174E-5 0.0 22-23 0.0 0.0 0.0 3.6128016011936694E-4 0.0 24-25 0.0 0.0 0.0 9.032004002984174E-4 0.0 26-27 0.0 0.0 0.0 0.0016257607205371512 0.0 28-29 0.0 0.0 0.0 0.0032515214410743024 0.0 30-31 0.0 0.0 0.0 0.007677203402536548 0.0 32-33 0.0 0.0 0.0 0.013638326044506103 0.0 34-35 0.0 0.0 0.0 0.0175220877657893 0.0 36-37 0.0 0.0 0.0 0.0218574496872217 0.0 38-39 0.0 0.0 0.0 0.02592185148856458 0.0 40-41 0.0 0.0 0.0 0.031882974130534134 0.0 42-43 0.0 0.0 0.0 0.04678578073545803 0.0 44-45 0.0 0.0 0.0 0.0672884298222321 0.0 46-47 0.0 0.0 0.0 0.08959747970960301 0.0 48-49 0.0 0.0 0.0 0.13728646084535945 0.0 50-51 0.0 0.0 0.0 0.17928527945923586 0.0 52-53 0.0 0.0 0.0 0.22390337923397768 0.0 54-55 0.0 0.0 0.0 0.3136814990236404 0.0 56-57 0.0 0.0 0.0 0.395511455290677 0.0 58-59 0.0 0.0 0.0 0.49052813740207046 0.0 60-61 0.0 0.0 0.0 0.5920478623956127 0.0 62-63 0.0 0.0 0.0 0.7238248007991517 0.0 64-65 0.0 0.0 0.0 0.8345571698757377 0.0 66-67 0.0 0.0 0.0 0.9851206766054839 0.0 68-69 0.0 0.0 0.0 1.1663026769053464 0.0 70-71 0.0 0.0 0.0 1.2981699353489153 0.0 72-73 0.0 0.0 0.0 1.5025641859364471 0.0 74-75 0.0 0.0 0.0 1.7540151773795265 0.0 76-77 0.0 0.0 0.0 1.9655447111294158 0.0 78-79 0.0 0.0 0.0 2.166416480155784 0.0 80-81 1.8064008005968347E-4 0.0 0.0 2.4492085254892184 0.0 82-83 1.8064008005968347E-4 0.0 0.0 2.7658705858338437 0.0 84-85 1.8064008005968347E-4 0.0 0.0 3.104028815705571 0.0 86-87 1.8064008005968347E-4 0.0 0.0 3.547680852332154 0.0 88-89 1.8064008005968347E-4 0.0 0.0 4.0787626877076235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3255 0.0 46.4246 1 GTATCAA 5095 0.0 35.348183 1 ATCGCGT 30 0.003949933 31.668232 24-25 GTCCGTG 80 4.4893645E-4 29.688967 7 GTCTAGA 100 4.934691E-5 28.511711 1 TGTCCGT 85 6.4044824E-4 27.940033 6 AGGTAAG 155 4.5722118E-8 27.5845 2 TATCAAC 6560 0.0 27.301832 2 TCCGACC 70 0.008385855 27.1442 8 CGGTTAC 70 0.008385855 27.1442 9 ATCAACG 6730 0.0 26.753366 3 TCAACGC 6790 0.0 26.446995 4 CCTTATA 180 6.672053E-9 26.392576 2 CCCACGT 90 8.939085E-4 26.390192 7 CAACGCA 6895 0.0 26.044249 5 AACGCAG 7025 0.0 25.557674 6 GTATGAC 115 1.2793654E-4 24.786074 3 CATGGGT 405 0.0 24.633074 4 GTACATG 4265 0.0 24.511827 1 TAAGGTG 235 1.7462298E-10 24.258709 5 >>END_MODULE