FastQCFastQC Report
Thu 26 May 2016
SRR937801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492632
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68761.3957680378050958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50571.0265269004043587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.7066126439208171No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.2515061952938502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.18857889865051397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.18512804689910523No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.13011741015605968No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6020.12220075025576901No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.1098182822065964No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.1098182822065964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.050.7095451
GTATCAA41900.041.7310521
TAAGGTG1404.5292836E-1033.938885
ATCAACG53200.032.956443
TATCAAC53350.032.7747152
TCAACGC54200.032.3483854
CAACGCA55000.031.8778655
AACGCAG55950.031.4151336
GTACATG34300.029.5060461
TACATGG34750.028.4402942
CGCAGAG63300.027.5422638
ACGCAGA63350.027.5205277
ACATGGG35500.026.7686923
GTGGTAT10150.026.6829811
GCAGAGT65450.026.419779
ATGGGAG8200.025.4955485
CATGGGA24250.025.2757194
GTATTAC950.00122338925.0075951
CTAAGAC950.00122338925.0075953
GAGTACT41850.024.91335512-13
Produced by FastQC (version 0.11.5)