##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937801_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 492632 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.868932590655906 33.0 31.0 34.0 30.0 34.0 2 31.958510612384092 33.0 31.0 34.0 30.0 34.0 3 31.944406778284804 34.0 31.0 34.0 30.0 34.0 4 35.56998327351857 37.0 35.0 37.0 33.0 37.0 5 35.57550666623362 37.0 35.0 37.0 33.0 37.0 6 35.644680004547006 37.0 35.0 37.0 33.0 37.0 7 35.59534094415304 37.0 35.0 37.0 33.0 37.0 8 35.580678884035144 37.0 35.0 37.0 33.0 37.0 9 37.21188635736209 39.0 37.0 39.0 34.0 39.0 10-11 37.10951176537456 39.0 37.0 39.0 34.0 39.0 12-13 36.97516503191023 39.0 37.0 39.0 33.0 39.0 14-15 38.05749727991685 40.0 38.0 41.0 33.0 41.0 16-17 38.01811900160769 40.0 38.0 41.0 33.0 41.0 18-19 38.052537188002404 40.0 38.0 41.0 33.5 41.0 20-21 38.024721292973254 40.0 38.0 41.0 33.0 41.0 22-23 37.82696921840238 40.0 38.0 41.0 33.0 41.0 24-25 37.66277566215756 40.0 37.0 41.0 32.5 41.0 26-27 37.576448545770475 40.0 37.0 41.0 32.5 41.0 28-29 37.57613796911285 40.0 37.0 41.0 33.0 41.0 30-31 37.29402779356599 39.5 36.5 41.0 32.0 41.0 32-33 36.94686195781029 39.0 35.5 41.0 31.5 41.0 34-35 36.898121721690835 39.0 35.0 41.0 31.0 41.0 36-37 36.764560564478145 39.0 35.0 41.0 31.0 41.0 38-39 36.597729136556296 39.0 35.0 41.0 31.0 41.0 40-41 36.30022816219815 39.0 35.0 41.0 30.0 41.0 42-43 35.949947425258614 39.0 35.0 41.0 30.0 41.0 44-45 35.56090347358678 39.0 35.0 41.0 28.0 41.0 46-47 34.96791722827588 39.0 35.0 40.5 24.5 41.0 48-49 34.43655913541954 38.5 34.5 40.0 21.5 41.0 50-51 33.4185832426639 37.5 33.5 39.5 18.5 40.5 52-53 33.486686816934345 38.0 33.5 39.5 18.0 40.5 54-55 33.7969549278163 38.0 34.0 40.0 15.5 41.0 56-57 33.56832077493951 38.0 34.0 40.0 12.5 41.0 58-59 33.309381038990566 38.0 33.5 40.0 8.5 41.0 60-61 33.00484134201595 37.5 33.0 40.0 7.5 41.0 62-63 32.64201980382923 37.0 33.0 40.0 4.0 41.0 64-65 32.2704036684584 36.0 32.0 39.5 2.0 41.0 66-67 31.854476566686696 36.0 31.5 39.0 2.0 41.0 68-69 31.38570373016775 35.0 31.0 39.0 2.0 40.0 70-71 30.951254689098555 35.0 31.0 38.0 2.0 40.0 72-73 30.506307954010296 35.0 30.5 37.5 2.0 40.0 74-75 30.06114706312217 35.0 30.0 37.0 2.0 39.0 76-77 29.585559809350592 34.5 29.0 36.5 2.0 39.0 78-79 29.086408312898875 34.0 29.0 36.0 2.0 38.5 80-81 28.587425299209144 34.0 28.5 35.5 2.0 37.0 82-83 28.086307832215528 34.0 27.0 35.0 2.0 37.0 84-85 27.640347155686193 34.0 26.0 35.0 2.0 36.5 86-87 27.19234844671073 33.5 26.0 35.0 2.0 36.0 88-89 26.730526031601684 33.0 25.0 35.0 2.0 35.5 90-91 26.263579101641795 33.0 24.5 35.0 2.0 35.0 92-93 25.736666517806395 32.5 22.0 35.0 2.0 35.0 94-95 25.144189780607025 32.0 19.0 35.0 2.0 35.0 96-97 24.470261777554036 32.0 8.5 34.0 2.0 35.0 98-99 23.607756905763328 31.5 2.0 34.0 2.0 35.0 100-101 21.374647810130078 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23174789107685 1101 2 0.3512656748838019 1101 3 0.308320839673069 1101 4 0.32824858176037 1101 5 0.36872763544774756 1101 6 0.3731072367182975 1101 7 0.3965521586980003 1101 8 0.26701530348543656 1101 9 0.32429590796273544 1101 10-11 0.39740071326907866 1101 12-13 0.47294139565095605 1101 14-15 0.6653861277910735 1101 16-17 0.6471535271391957 1101 18-19 0.6805176909753854 1101 20-21 0.7310648124077872 1101 22-23 0.4380868576835084 1101 24-25 0.6090401372035785 1101 26-27 0.647859414453805 1101 28-29 0.5015193825793673 1101 30-31 0.6191146030623216 1101 32-33 0.6089939983461932 1101 34-35 0.9315544276378773 1101 36-37 0.8600420630328358 1101 38-39 0.8629510111339016 1101 40-41 1.1400585340299685 1101 42-43 1.0220777867959399 1101 44-45 0.9807577278303157 1101 46-47 1.0248171365341534 1101 48-49 1.2417664297936852 1101 50-51 1.140323653372576 1101 52-53 1.2303282306364025 1101 54-55 1.1757485138808903 1101 56-57 0.8562796258355334 1101 58-59 1.212013983862498 1101 60-61 1.0944189660115242 1101 62-63 1.3962391576974156 1101 64-65 1.2533865681958538 1101 66-67 1.5081871975882102 1101 68-69 1.365198759961448 1101 70-71 1.5463553898606186 1101 72-73 1.4560464495350978 1101 74-75 1.7009999903732762 1101 76-77 1.6050570816841265 1101 78-79 1.4300978645429012 1101 80-81 1.7840162978855396 1101 82-83 1.4810559772937744 1101 84-85 1.8138173571709721 1101 86-87 1.7719357793094233 1101 88-89 1.7100968721363543 1101 90-91 1.7812234760811272 1101 92-93 1.8658239256581446 1101 94-95 1.8967386283206622 1101 96-97 1.9854436230927952 1101 98-99 1.9002017872172203 1101 100-101 2.523013652815635 1102 1 0.2580859734796235 1102 2 0.13748053818761363 1102 3 0.2651684843913351 1102 4 0.307891759786294 1102 5 0.2763518171959234 1102 6 0.24499787976797194 1102 7 0.1987983401894624 1102 8 0.33320706320819227 1102 9 0.25013341277598045 1102 10-11 0.25364509781534395 1102 12-13 0.268686486139984 1102 14-15 0.44425276878710207 1102 16-17 0.5208090270750247 1102 18-19 0.641286526811605 1102 20-21 0.5869498079154241 1102 22-23 0.5478224500336566 1102 24-25 0.5622553855670773 1102 26-27 0.4862691179578533 1102 28-29 0.3765675150398877 1102 30-31 0.6305380403617775 1102 32-33 0.5054963728809128 1102 34-35 0.785687401582166 1102 36-37 0.9264712762275451 1102 38-39 0.8342255586887717 1102 40-41 0.8688417454277371 1102 42-43 0.9396557613820065 1102 44-45 0.8993368575954364 1102 46-47 1.2379476717671167 1102 48-49 1.602361988771996 1102 50-51 1.4636133466083692 1102 52-53 1.3439400904746819 1102 54-55 1.2996350496272626 1102 56-57 1.3740501302637185 1102 58-59 1.3566937425584271 1102 60-61 1.347377507918857 1102 62-63 1.5009818094751068 1102 64-65 1.2821697795936196 1102 66-67 1.5357061296893555 1102 68-69 1.6845480090950709 1102 70-71 1.623059278963428 1102 72-73 1.529778961407775 1102 74-75 1.7139636664097253 1102 76-77 1.704235621024388 1102 78-79 1.9608384626689457 1102 80-81 1.9513504013382388 1102 82-83 1.8483130688096274 1102 84-85 1.9039854998997576 1102 86-87 2.01190497453468 1102 88-89 2.1217256746007394 1102 90-91 1.903967668097554 1102 92-93 1.857564395430959 1102 94-95 2.198952721505105 1102 96-97 2.084878868889227 1102 98-99 2.4845811993594396 1102 100-101 3.283493693888669 1103 1 0.009063025909089362 1103 2 0.07531460960070291 1103 3 -0.005468328755771523 1103 4 -0.06994520200904475 1103 5 -0.010068547611609802 1103 6 0.03031267336371002 1103 7 0.030635900706840857 1103 8 0.05609579381424368 1103 9 0.1185115986482046 1103 10-11 0.0804028115229869 1103 12-13 0.06559893852610088 1103 14-15 -0.05090274410090245 1103 16-17 -0.034863864236285735 1103 18-19 -0.18015804494729792 1103 20-21 -0.021316685328308438 1103 22-23 0.0300920194699259 1103 24-25 -0.054650253775157864 1103 26-27 -0.06291796203348099 1103 28-29 -0.019874217950722084 1103 30-31 0.08104540567511975 1103 32-33 -0.33309848843235557 1103 34-35 0.024526913107564496 1103 36-37 -0.008803774612211157 1103 38-39 0.12375824581521755 1103 40-41 0.031001480338396448 1103 42-43 -0.11561041185512266 1103 44-45 -0.22931818560545736 1103 46-47 -0.43182099442469735 1103 48-49 -0.09269442529591032 1103 50-51 -0.08322116868485807 1103 52-53 -0.12065995288105569 1103 54-55 0.007354434801662535 1103 56-57 -0.06230055568592263 1103 58-59 -0.20546083095535295 1103 60-61 0.028894545689958306 1103 62-63 0.1627306971940783 1103 64-65 0.2725540972053402 1103 66-67 0.12151744966499578 1103 68-69 0.25331949340958104 1103 70-71 0.3899107236384154 1103 72-73 0.22800653771902546 1103 74-75 0.263074357727227 1103 76-77 0.23542491009358457 1103 78-79 0.29880156900404486 1103 80-81 0.2170787260520406 1103 82-83 0.27544549993078604 1103 84-85 0.32470128241914153 1103 86-87 0.5692088672602083 1103 88-89 0.5879236864306598 1103 90-91 1.020874339719164 1103 92-93 0.9385807777046544 1103 94-95 0.9276449267635343 1103 96-97 1.3950571435374783 1103 98-99 1.3859087106370893 1103 100-101 1.74056945287208 1104 1 0.34239392743042885 1104 2 0.1837782880567893 1104 3 0.3878529427900794 1104 4 0.22212514544825268 1104 5 0.3685548522560964 1104 6 0.15563367254662097 1104 7 0.005712259603377845 1104 8 0.1280069062223248 1104 9 0.37372053993453136 1104 10-11 0.24803612345637305 1104 12-13 0.30214737049372786 1104 14-15 0.479169042989092 1104 16-17 0.5922408690425769 1104 18-19 0.5100692322014524 1104 20-21 0.4563179397835526 1104 22-23 0.37143574150409364 1104 24-25 0.38739601874342355 1104 26-27 0.33256032674905356 1104 28-29 0.38325576726385435 1104 30-31 0.47391192837852003 1104 32-33 0.37951848172444613 1104 34-35 0.4118549839997456 1104 36-37 0.6181477545111704 1104 38-39 0.5749797449786698 1104 40-41 0.7360676742608092 1104 42-43 0.6332356933547985 1104 44-45 0.7024536812479738 1104 46-47 0.8755065414688517 1104 48-49 1.0571173524559256 1104 50-51 0.9204095266188332 1104 52-53 0.8620948523794354 1104 54-55 1.1257666561160136 1104 56-57 1.4243281260774339 1104 58-59 1.1953188079692083 1104 60-61 1.2497748129947368 1104 62-63 1.2432698785488938 1104 64-65 1.1210303238113148 1104 66-67 0.9886626969316374 1104 68-69 1.0276667950709957 1104 70-71 0.9618838472522846 1104 72-73 0.9643616406333102 1104 74-75 1.0776803069120788 1104 76-77 1.1137005608464747 1104 78-79 1.282577735301075 1104 80-81 1.2485050324214413 1104 82-83 1.1880631996311877 1104 84-85 1.421253945739636 1104 86-87 1.2948360315728813 1104 88-89 1.31942975623339 1104 90-91 1.4286831312058759 1104 92-93 1.4661332075712004 1104 94-95 1.4496297230749633 1104 96-97 1.773680543405188 1104 98-99 2.227405636826731 1104 100-101 2.92466977970761 1105 1 -0.09058488975761847 1105 2 -0.015777149956406333 1105 3 0.08770433566701286 1105 4 0.057070488252144 1105 5 -0.09656025529515944 1105 6 0.036489800701374975 1105 7 0.08099499095192897 1105 8 -0.33397548761173823 1105 9 -0.22740156978286308 1105 10-11 -0.10326387402486148 1105 12-13 -0.10443006892997886 1105 14-15 -0.14073045829548647 1105 16-17 0.02867826514353311 1105 18-19 -0.0645098192317235 1105 20-21 -0.02543675771279652 1105 22-23 0.054216205964323194 1105 24-25 -0.09194853568708083 1105 26-27 -0.1586649263868054 1105 28-29 0.21338239816406457 1105 30-31 0.29546059091440924 1105 32-33 0.2851136028770398 1105 34-35 0.24464520867356754 1105 36-37 0.22190984458219987 1105 38-39 0.3193424345157254 1105 40-41 0.4369344441968295 1105 42-43 0.7507659815014875 1105 44-45 0.5942207836609512 1105 46-47 0.9058639541936486 1105 48-49 0.8969144659514683 1105 50-51 1.0133173146395862 1105 52-53 0.9029633854508248 1105 54-55 1.1603650390690134 1105 56-57 1.1700005733093022 1105 58-59 1.11788882963058 1105 60-61 1.0149676874091185 1105 62-63 1.0032284448679647 1105 64-65 0.9962349734511271 1105 66-67 1.1261368795924618 1105 68-69 1.0264829756160587 1105 70-71 1.2363426276663674 1105 72-73 1.2499171542094523 1105 74-75 1.1956938830543393 1105 76-77 1.1654895135440668 1105 78-79 1.1863373066073066 1105 80-81 1.2981341443049743 1105 82-83 1.2005657183959855 1105 84-85 1.3781578800502494 1105 86-87 1.4756754729108508 1105 88-89 1.1859572611449813 1105 90-91 1.5818287535926565 1105 92-93 1.5516263817185951 1105 94-95 1.5225243505496522 1105 96-97 1.7574848857557015 1105 98-99 1.9894232933679632 1105 100-101 2.527085904839847 1106 1 0.25095748828272235 1106 2 0.29333950149124277 1106 3 0.33630003560336874 1106 4 0.019705499366637014 1106 5 0.18220598985089964 1106 6 0.03686335835377008 1106 7 0.2468868713765815 1106 8 -0.1318116203215567 1106 9 -0.056985491123612064 1106 10-11 -0.046824385752728404 1106 12-13 -0.11818713654852786 1106 14-15 -0.1441155362814044 1106 16-17 -0.22081212842943643 1106 18-19 -0.22418726834025904 1106 20-21 -0.08085703313085446 1106 22-23 -0.2303609359968064 1106 24-25 -0.41484531386762313 1106 26-27 -0.3296597888161301 1106 28-29 0.07485246282182345 1106 30-31 0.4328998301481093 1106 32-33 0.27403139252256636 1106 34-35 0.2241179850831685 1106 36-37 0.31220472453644277 1106 38-39 0.5558505467121506 1106 40-41 0.8284182520867418 1106 42-43 0.9414809985191184 1106 44-45 0.9486278415946643 1106 46-47 1.1105643240618406 1106 48-49 0.9238336760384485 1106 50-51 0.9385343009055802 1106 52-53 1.0520889838348424 1106 54-55 1.159781643476073 1106 56-57 1.10942427992358 1106 58-59 1.0065548137474636 1106 60-61 0.8875614688271654 1106 62-63 1.3353834574582422 1106 64-65 1.3138120139521554 1106 66-67 1.4911004000735666 1106 68-69 1.0198119738358962 1106 70-71 1.4268870072378341 1106 72-73 1.2384452448225467 1106 74-75 1.6204981111735464 1106 76-77 1.6254150787229626 1106 78-79 1.2655476702956605 1106 80-81 1.1985095466865197 1106 82-83 0.8959225351313655 1106 84-85 0.617624476617209 1106 86-87 0.9928429835411023 1106 88-89 0.9723053430878501 1106 90-91 0.9547868690173615 1106 92-93 0.8776084693985702 1106 94-95 1.2802883543497927 1106 96-97 1.077942009638484 1106 98-99 1.0738980528614022 1106 100-101 1.6015161141243688 1107 1 0.05031382285032748 1107 2 -0.0580868288083245 1107 3 0.3068506117877483 1107 4 0.30109572441882904 1107 5 0.08152656522781143 1107 6 0.02746791938086801 1107 7 0.16877611016722938 1107 8 0.3467155624309797 1107 9 0.26053305174704633 1107 10-11 0.28763363180387813 1107 12-13 0.24806772266408217 1107 14-15 0.3613983002183474 1107 16-17 0.5844387049904114 1107 18-19 0.6516677800499977 1107 20-21 0.6091915301571476 1107 22-23 0.5676573127256646 1107 24-25 0.7569602945577003 1107 26-27 0.43881387050259946 1107 28-29 0.3901562429143368 1107 30-31 0.6514894184560021 1107 32-33 0.7173819133378814 1107 34-35 0.5866585025532629 1107 36-37 0.6761294687428858 1107 38-39 0.4435320822810098 1107 40-41 0.774542993986131 1107 42-43 0.7097354886045864 1107 44-45 0.6715777441220396 1107 46-47 0.8172236081859126 1107 48-49 0.7870951449337227 1107 50-51 0.7900269587362629 1107 52-53 1.0604722070067893 1107 54-55 0.7999893928387465 1107 56-57 0.7896044601037673 1107 58-59 0.7749390750894705 1107 60-61 0.8489703809403011 1107 62-63 0.5832379060169117 1107 64-65 0.5291486694296559 1107 66-67 0.7080289162978595 1107 68-69 0.6618884292926275 1107 70-71 0.9025909442093791 1107 72-73 1.0756630644347318 1107 74-75 1.1160512827830544 1107 76-77 0.9101591698050839 1107 78-79 1.374500733474072 1107 80-81 1.1404008618172732 1107 82-83 1.1398405889085765 1107 84-85 0.9834035078891965 1107 86-87 1.1478273533142058 1107 88-89 1.3592024835061167 1107 90-91 0.9931184143911587 1107 92-93 0.956486322924313 1107 94-95 1.6214368011320417 1107 96-97 1.723752993477639 1107 98-99 1.4696369415580364 1107 100-101 1.7507069182447488 1108 1 0.066778339314844 1108 2 0.11087434015284359 1108 3 0.17000597494311265 1108 4 0.16148208480519344 1108 5 0.22691871061995528 1108 6 0.030938922851866835 1108 7 0.10250204389316764 1108 8 0.031166246881660697 1108 9 0.20924150045549794 1108 10-11 0.25496784913810444 1108 12-13 -0.04457881998246904 1108 14-15 0.012695701515745839 1108 16-17 -0.12767925712754646 1108 18-19 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377.0 4 361.0 5 395.0 6 446.0 7 566.0 8 597.0 9 771.0 10 1069.0 11 1472.0 12 2132.0 13 3055.0 14 3483.0 15 4658.0 16 6307.0 17 9289.0 18 7571.0 19 4394.0 20 4003.0 21 4019.0 22 4129.0 23 4485.0 24 4812.0 25 5281.0 26 5983.0 27 7042.0 28 8291.0 29 9721.0 30 11575.0 31 14575.0 32 18293.0 33 24422.0 34 33229.0 35 48459.0 36 66799.0 37 90076.0 38 69637.0 39 8903.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.60377961215026 18.610764651185505 15.459256651861015 20.326199084803218 2 15.454305013020834 20.399373372395832 41.3690185546875 22.777303059895836 3 18.762473019625233 26.705332603684994 30.648078264955924 23.884116111733846 4 12.056224000976394 17.05230825561172 37.65848597959744 33.23298176381444 5 12.552172745689901 38.01694749774223 33.65152267124458 15.779357085323287 6 29.76544419996014 36.733789131610735 17.76535472141669 15.735411947012434 7 26.874922473447793 35.15709675517064 20.551399341558096 17.416581429823474 8 27.113939186880764 33.36790472936698 19.666243675468138 19.851912408284118 9 27.625716104392104 16.235360151955472 20.717355096263546 35.421568647388874 10-11 24.66702189754301 27.430297804144406 26.33043793052254 21.57224236779005 12-13 26.476064384198235 25.284189996227408 27.75508460874367 20.484661010830692 14-15 23.21842025789093 25.508953783610373 27.950589716291503 23.322036242207194 16-17 20.36244874053994 29.357168495043606 28.299695874941662 21.980686889474793 18-19 20.351267505671263 25.97626559915076 33.40691655134336 20.26555034383462 20-21 21.51203636581768 24.77883933990876 33.80441070762261 19.904713586650953 22-23 21.164427292173304 24.957096729591463 33.887991964278456 19.99048401395678 24-25 20.698461757439585 25.08341890421822 34.03115119104505 20.186968147297147 26-27 20.19675984765143 25.509235064573264 33.995571073292844 20.29843401448247 28-29 20.11744011062757 25.483487208687517 34.30339203644202 20.09568064424289 30-31 20.17821411722601 25.274926388052904 34.2289453563469 20.317914138374192 32-33 19.844329886424873 25.59263439384234 34.00227760322932 20.56075811650347 34-35 20.529870679180863 25.468036843063153 33.812122339792765 20.189970137963222 36-37 20.61094002738921 25.291302654842063 33.62427167982074 20.473485637947984 38-39 20.31022033248641 25.63130427356163 33.557315829553694 20.50115956439827 40-41 20.327977185963878 25.610585550093788 33.6689016424276 20.392535621514735 42-43 20.410298435835074 25.79102984358351 33.01634523877307 20.782326481808347 44-45 20.311491260086765 25.9008063310868 32.90656380548419 20.881138603342244 46-47 20.55271109448265 26.40394148769607 32.180648808240846 20.862698609580434 48-49 20.818806562897485 26.50455655095071 31.491903136562314 21.184733749589487 50-51 20.961691959632933 26.99875441673657 30.951473092859505 21.08808053077099 52-53 20.969605023936094 27.08795142009981 30.731443934223527 21.210999621740562 54-55 20.833430767213006 27.072723631087882 30.724816561658365 21.36902904004075 56-57 20.938589624991106 27.296662634312955 30.576897662929113 21.18785007776682 58-59 20.92103805109739 26.837364065100328 30.776667740895906 21.464930142906375 60-61 20.935455502066418 26.828591326626576 31.166396396854363 21.069556774452643 62-63 20.878658322700026 26.847678550549286 31.292823409198363 20.980839717552325 64-65 20.72633780207737 26.926326212553892 31.119802124135997 21.22753386123274 66-67 20.757120562833283 27.119190317152892 30.886289580569237 21.23739953944459 68-69 20.72937011532584 27.021811560238767 31.04067770072217 21.20814062371322 70-71 20.829317489558807 27.211154082333948 30.708903176481595 21.250625251625653 72-73 20.794229332194654 27.35536475886925 30.37327355263091 21.477132356305187 74-75 20.562758239255622 27.66901454273351 30.29634479617399 21.471882421836874 76-77 20.67132204465177 27.466187843281592 30.42077350582905 21.44171660623758 78-79 20.71566056556138 27.344753290092456 30.351821027918124 21.58776511642804 80-81 20.637338235413736 27.76718100552297 30.166909330491865 21.428571428571427 82-83 20.769680486006965 27.57469306830025 30.06420782389873 21.591418621794055 84-85 20.635464494430458 27.24296537896873 29.97059672781842 22.150973398782387 86-87 20.855925422467404 27.32543893806929 29.79563161431809 22.023004025145212 88-89 20.760675392228332 27.22605020400652 29.88499786995436 22.12827653381079 90-91 20.603178056787762 27.296435285397795 30.120877590215922 21.979509067598524 92-93 20.873878700935062 27.035418969080972 30.14704611471081 21.94365621527315 94-95 20.40981698135652 27.119517752330562 30.052228585720663 22.418436680592258 96-97 20.631151115778472 27.072279835675428 30.00570317273294 22.29086587581316 98-99 20.73898987434427 27.249095197429956 29.553088528323368 22.458826399902403 100-101 20.95074754022362 26.67375608208811 30.07169391618359 22.303802461504677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1081.0 1 699.0 2 457.0 3 582.5 4 451.0 5 368.0 6 682.5 7 1579.5 8 2707.0 9 3097.0 10 2487.5 11 1866.5 12 1727.5 13 1755.0 14 1767.5 15 1812.0 16 1855.5 17 1799.5 18 1813.5 19 1843.5 20 1897.5 21 2043.0 22 2315.5 23 2789.0 24 3146.0 25 3456.5 26 4007.5 27 4636.0 28 5113.5 29 5750.0 30 6654.0 31 7581.5 32 8597.5 33 9378.0 34 10046.0 35 10809.0 36 11726.5 37 12618.5 38 13138.5 39 13278.0 40 13599.5 41 14454.0 42 14779.0 43 15257.5 44 16392.5 45 17402.5 46 17372.0 47 17189.0 48 17862.0 49 17345.0 50 16419.0 51 15626.5 52 14455.0 53 14178.0 54 14110.5 55 13027.5 56 11677.0 57 10628.5 58 9802.0 59 8751.0 60 7325.5 61 6057.0 62 4985.5 63 3831.0 64 2812.0 65 2166.5 66 1619.0 67 1254.0 68 912.5 69 603.0 70 448.5 71 324.0 72 248.5 73 216.5 74 173.5 75 135.5 76 104.0 77 66.5 78 40.0 79 25.0 80 18.5 81 15.0 82 11.5 83 7.0 84 5.0 85 3.5 86 3.0 87 2.5 88 2.5 89 4.5 90 3.5 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23404894525731174 2 0.2257263027980318 3 0.21740366033875186 4 0.20847204404098799 5 0.20217931437665437 6 0.18147420386820182 7 0.17558745676285747 8 0.1818801864271911 9 0.18451907306062132 10-11 0.18939086376849248 12-13 0.18827441173127202 14-15 0.18522954253885254 16-17 0.1813727082284545 18-19 0.18238766462592768 20-21 0.17284707448967992 22-23 0.16807677942155605 24-25 0.16990370093700774 26-27 0.17558745676285747 28-29 0.18106822130921257 30-31 0.17538446548336284 32-33 0.18005326491173937 34-35 0.17670390880007794 36-37 0.16899024017928188 38-39 0.17305006576917456 40-41 0.168381266340798 42-43 0.17619643060134135 44-45 0.16980220529726045 46-47 0.17010669221650238 48-49 0.17609493496159406 50-51 0.18157569950794913 52-53 0.1839100992221374 54-55 0.1716291268127121 56-57 0.15772422416732976 58-59 0.16371246691242144 60-61 0.17041117913574433 62-63 0.17447100472563698 64-65 0.16411844947141072 66-67 0.16939622273827115 68-69 0.17284707448967992 70-71 0.16848276198054532 72-73 0.16939622273827115 74-75 0.17051267477549165 76-77 0.17680540443982526 78-79 0.1807637343899706 80-81 0.17619643060134135 82-83 0.17650091752058333 84-85 0.17254258757043797 86-87 0.17223810065119602 88-89 0.17426801344614234 90-91 0.1732530570486692 92-93 0.17203510937170138 94-95 0.17284707448967992 96-97 0.16249451923545363 98-99 0.1648289189496419 100-101 0.16624985790610436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 492632.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.874072341262355 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3468951013965 48.70569882038094 2 10.742153554538 12.863529580507132 3 3.2496398444330286 5.83707513385895 4 1.4489658772934808 3.4702195102836217 5 0.7861295900776469 2.353438997295797 6 0.4829259306162636 1.7348845263113766 7 0.3479906924456676 1.4584933925504517 8 0.24485140547156767 1.172820061125152 9 0.1798074892321295 0.9689225956006745 >10 1.072855089095162 11.703935870563718 >50 0.06519028376615627 2.640560707901535 >100 0.029199814380802276 2.8580832065515 >500 0.002037196352148996 0.8469147619669641 >1k 6.79065450716332E-4 0.9595385685271414 >5k 6.79065450716332E-4 2.42588426657508 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6876 1.3957680378050958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5057 1.0265269004043587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3481 0.7066126439208171 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.2515061952938502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 929 0.18857889865051397 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 912 0.18512804689910523 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.13011741015605968 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 602 0.12220075025576901 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.1098182822065964 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.1098182822065964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.0299127949463292E-4 0.0 24-25 2.0299127949463292E-4 0.0 0.0 8.119651179785317E-4 0.0 26-27 2.0299127949463292E-4 0.0 0.0 0.001116452037220481 0.0 28-29 2.0299127949463292E-4 0.0 0.0 0.003653843030903392 0.0 30-31 2.0299127949463292E-4 0.0 0.0 0.008931616297763848 0.0 32-33 2.0299127949463292E-4 0.0 0.0 0.015833319800581367 0.0 34-35 2.0299127949463292E-4 0.0 0.0 0.021111093067441824 0.0 36-37 2.0299127949463292E-4 0.0 0.0 0.027099335812533494 0.0 38-39 2.0299127949463292E-4 0.0 0.0 0.03237710907939395 0.0 40-41 2.0299127949463292E-4 0.0 0.0 0.03917731694246415 0.0 42-43 3.0448691924194935E-4 0.0 0.0 0.05348820214683577 0.0 44-45 4.0598255898926584E-4 0.0 0.0 0.07327985189756248 0.0 46-47 4.0598255898926584E-4 0.0 0.0 0.09631936212020331 0.0 48-49 4.0598255898926584E-4 0.0 0.0 0.14503726919891521 0.0 50-51 4.0598255898926584E-4 0.0 0.0 0.19020282888647105 0.0 52-53 4.0598255898926584E-4 0.0 0.0 0.24084915312038196 0.0 54-55 4.0598255898926584E-4 0.0 0.0 0.3281354033030741 0.0 56-57 4.0598255898926584E-4 0.0 0.0 0.41298575813183064 0.0 58-59 4.0598255898926584E-4 0.0 0.0 0.5159023368356095 0.0 60-61 4.0598255898926584E-4 0.0 0.0 0.625416132122964 0.0 62-63 4.0598255898926584E-4 0.0 0.0 0.7745132269117718 0.0 64-65 4.0598255898926584E-4 0.0 0.0 0.8872748826710404 0.0 66-67 4.0598255898926584E-4 0.0 0.0 1.0384018902547947 0.0 68-69 4.0598255898926584E-4 0.0 0.0 1.238957274395492 0.0 70-71 4.0598255898926584E-4 0.0 0.0 1.372424040663213 0.0 72-73 4.0598255898926584E-4 0.0 0.0 1.5938875265918577 0.0 74-75 4.0598255898926584E-4 0.0 0.0 1.8735080140957145 0.0 76-77 4.0598255898926584E-4 0.0 0.0 2.08735932704331 0.0 78-79 4.0598255898926584E-4 0.0 0.0 2.2963388492830346 0.0 80-81 4.0598255898926584E-4 0.0 0.0 2.577786258302343 0.0 82-83 6.089738384838987E-4 0.0 0.0 2.9155637473814124 0.0 84-85 6.089738384838987E-4 0.0 0.0 3.2648102437519286 0.0 86-87 6.089738384838987E-4 0.0 0.0 3.7269198915214603 0.0 88-89 6.089738384838987E-4 0.0 0.0 4.291438639796034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2305 0.0 50.709545 1 GTATCAA 4190 0.0 41.731052 1 TAAGGTG 140 4.5292836E-10 33.93888 5 ATCAACG 5320 0.0 32.95644 3 TATCAAC 5335 0.0 32.774715 2 TCAACGC 5420 0.0 32.348385 4 CAACGCA 5500 0.0 31.877865 5 AACGCAG 5595 0.0 31.415133 6 GTACATG 3430 0.0 29.506046 1 TACATGG 3475 0.0 28.440294 2 CGCAGAG 6330 0.0 27.542263 8 ACGCAGA 6335 0.0 27.520527 7 ACATGGG 3550 0.0 26.768692 3 GTGGTAT 1015 0.0 26.682981 1 GCAGAGT 6545 0.0 26.41977 9 ATGGGAG 820 0.0 25.495548 5 CATGGGA 2425 0.0 25.275719 4 GTATTAC 95 0.001223389 25.007595 1 CTAAGAC 95 0.001223389 25.007595 3 GAGTACT 4185 0.0 24.913355 12-13 >>END_MODULE