FastQCFastQC Report
Thu 26 May 2016
SRR937801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492632
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56301.1428409035547833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.9221893827441173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33800.6861105246918593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.3180873349680898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.2326280063008493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.1833011253836535No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.11225417756053199No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.1073823868526608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.045.1120031
GTATCAA47250.038.2118341
GTACCGG650.005832824429.2390161
TATCAAC61850.029.0292592
ATCAACG61800.028.975893
TCAACGC62150.028.8891354
CAACGCA63050.028.5520955
AGGTAAG1503.3292963E-828.4993612
AACGCAG64100.028.0843936
CGCGTAT856.402781E-427.940557
CTAGTAC700.00838791627.1422483
GGTAAGG1806.681148E-926.3882963
ACGCGTA908.9414313E-426.3882966
TACGCGT950.0012257224.999445
ACGCAGA72900.024.6942337
CGCAGAG72800.024.6629078
GAGTACT42550.024.05645212-13
AGTACTT43750.023.61375412-13
GCAGAGT76650.023.3621629
CTAGATC1452.3312452E-522.9305214