##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937801_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 492632 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27165307978369 33.0 31.0 34.0 31.0 34.0 2 32.46418218873317 34.0 31.0 34.0 31.0 34.0 3 32.49776303609997 34.0 31.0 34.0 31.0 34.0 4 36.11694327611686 37.0 35.0 37.0 35.0 37.0 5 36.04677731044674 37.0 35.0 37.0 35.0 37.0 6 36.11614349047565 37.0 35.0 37.0 35.0 37.0 7 36.07050902093246 37.0 35.0 37.0 35.0 37.0 8 36.043925282969845 37.0 35.0 37.0 35.0 37.0 9 37.72780087367447 39.0 37.0 39.0 35.0 39.0 10-11 37.653270595495215 39.0 37.0 39.0 35.0 39.0 12-13 37.540995103850335 39.0 37.0 39.0 35.0 39.0 14-15 38.705532730313905 40.0 38.0 41.0 34.5 41.0 16-17 38.689047808506146 40.0 38.0 41.0 34.5 41.0 18-19 38.730580839247146 40.0 38.0 41.0 35.0 41.0 20-21 38.664644196885305 40.0 38.0 41.0 34.0 41.0 22-23 38.59373426817584 40.0 38.0 41.0 34.0 41.0 24-25 38.573882735997664 40.0 38.0 41.0 34.0 41.0 26-27 38.367708553240554 40.0 38.0 41.0 34.0 41.0 28-29 38.296760056187985 40.0 38.0 41.0 34.0 41.0 30-31 38.209386519755114 40.0 37.5 41.0 34.0 41.0 32-33 37.97660424008185 40.0 37.0 41.0 33.5 41.0 34-35 37.78965739131847 40.0 36.0 41.0 33.0 41.0 36-37 37.57164678705403 40.0 36.0 41.0 33.0 41.0 38-39 37.36896405430423 40.0 36.0 41.0 33.0 41.0 40-41 37.129623126390484 40.0 35.5 41.0 33.0 41.0 42-43 36.8206541596973 40.0 35.0 41.0 31.5 41.0 44-45 36.365322390750094 39.0 35.0 41.0 30.5 41.0 46-47 35.79209024180321 39.0 35.0 41.0 29.0 41.0 48-49 35.25206239139966 39.0 35.0 41.0 26.0 41.0 50-51 34.91557085207619 39.0 35.0 40.5 24.0 41.0 52-53 34.61552842689878 38.5 35.0 40.0 23.5 41.0 54-55 34.352518715795966 38.0 34.0 40.0 23.0 41.0 56-57 34.06353525552542 38.0 34.0 40.0 22.0 41.0 58-59 33.78192646843891 38.0 34.0 40.0 20.0 41.0 60-61 33.4228379398821 37.0 33.0 40.0 18.5 41.0 62-63 33.00249171795579 37.0 33.0 40.0 16.0 41.0 64-65 32.6018244856201 36.0 32.5 39.0 12.0 41.0 66-67 32.180970785495056 35.5 32.0 39.0 7.5 40.0 68-69 31.714546558079867 35.0 31.5 38.5 6.5 40.0 70-71 31.287944144919535 35.0 31.0 38.0 2.0 40.0 72-73 30.82482360057812 35.0 31.0 37.0 2.0 39.5 74-75 30.361878643693466 35.0 30.0 36.5 2.0 39.0 76-77 29.12967488916676 33.5 28.5 35.0 2.0 37.5 78-79 29.706986147875085 34.0 30.0 36.0 2.0 38.0 80-81 29.53251920297504 34.0 30.0 35.0 2.0 37.0 82-83 29.160684851978758 34.0 29.5 35.0 2.0 37.0 84-85 28.762354049270044 34.0 29.0 35.0 2.0 36.0 86-87 28.445459288068985 34.0 29.0 35.0 2.0 36.0 88-89 28.062988194027184 34.0 28.5 35.0 2.0 35.5 90-91 27.574734284415143 33.5 27.0 35.0 2.0 35.0 92-93 27.12599668718232 33.0 26.0 35.0 2.0 35.0 94-95 26.636773494210686 33.0 25.0 35.0 2.0 35.0 96-97 26.131441928254763 33.0 24.5 35.0 2.0 35.0 98-99 25.353053597817436 32.5 21.0 34.5 2.0 35.0 100-101 23.046418015882036 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2736091845581541 1101 2 0.2026257015853119 1101 3 0.24954612913930418 1101 4 0.18535164096930856 1101 5 0.17855699466535668 1101 6 0.08892546275698976 1101 7 0.15159333008134013 1101 8 0.22115420686797904 1101 9 0.2158675966757002 1101 10-11 0.1832568717279841 1101 12-13 0.26526058797419694 1101 14-15 0.3446535708180747 1101 16-17 0.410584618257694 1101 18-19 0.39263337948762 1101 20-21 0.34161622583970797 1101 22-23 0.16018962667948955 1101 24-25 0.15032802607581175 1101 26-27 0.14779147972215156 1101 28-29 0.3236417398315936 1101 30-31 0.430876085550814 1101 32-33 0.3012995364161739 1101 34-35 0.3790043880826133 1101 36-37 0.41816629006272166 1101 38-39 0.5787114444329617 1101 40-41 0.6045634314220862 1101 42-43 0.4867087237055401 1101 44-45 0.6883918714424695 1101 46-47 0.3823794353427772 1101 48-49 0.18938123224565828 1101 50-51 0.3922430622384425 1101 52-53 0.5079700953580257 1101 54-55 0.7302313573711388 1101 56-57 0.6041488451612835 1101 58-59 0.5984125096325599 1101 60-61 0.7083301636010688 1101 62-63 0.7438907721380943 1101 64-65 0.7755202599342041 1101 66-67 0.6974795344965159 1101 68-69 0.7147175831117956 1101 70-71 0.5843289354285552 1101 72-73 0.7000816019341229 1101 74-75 0.7909146194933179 1101 76-77 0.7728724775164117 1101 78-79 0.9987833999229778 1101 80-81 0.9460414338461263 1101 82-83 1.2667970157859187 1101 84-85 1.3426361996774894 1101 86-87 1.304474428438212 1101 88-89 1.4069583947713653 1101 90-91 1.3033691127621658 1101 92-93 1.2383878927461076 1101 94-95 1.1654925970900898 1101 96-97 1.0473836907900846 1101 98-99 0.9820264789673914 1101 100-101 1.3629867360955679 1102 1 0.22126673814562992 1102 2 0.25901127468247864 1102 3 0.3093458981034942 1102 4 0.11049603835090238 1102 5 0.08773425047333916 1102 6 0.09050420746241628 1102 7 0.06354301561593445 1102 8 0.017829858260611786 1102 9 0.344079636208491 1102 10-11 0.29909566007351174 1102 12-13 0.21818062391309923 1102 14-15 0.2793410307076911 1102 16-17 0.39188997058730735 1102 18-19 0.16882385567809877 1102 20-21 0.3094252362363221 1102 22-23 0.32767775400589016 1102 24-25 0.36702678104950337 1102 26-27 0.35913790508475785 1102 28-29 0.23860772622615656 1102 30-31 0.3498338573387727 1102 32-33 0.4160152340159726 1102 34-35 0.33012747882834503 1102 36-37 0.21481627080972743 1102 38-39 0.4753870958255888 1102 40-41 0.41490870160563276 1102 42-43 0.40922829757281676 1102 44-45 0.6593198652815175 1102 46-47 0.5841442922289275 1102 48-49 0.6187163625241823 1102 50-51 0.8467996018354071 1102 52-53 0.7433506626792905 1102 54-55 0.7968980240378016 1102 56-57 0.8993612697537614 1102 58-59 0.9481814738220109 1102 60-61 1.0233924149166853 1102 62-63 0.8701903485724429 1102 64-65 0.9666574695350292 1102 66-67 0.9465553912543143 1102 68-69 0.6323276460048781 1102 70-71 0.8563415140449031 1102 72-73 0.9598300296070796 1102 74-75 0.9895476576142279 1102 76-77 0.8608650330291816 1102 78-79 0.6772713988961527 1102 80-81 0.7774109627897765 1102 82-83 0.7448550314450166 1102 84-85 0.7714194110752572 1102 86-87 0.7974855951690571 1102 88-89 0.7164372806653425 1102 90-91 0.8815683169721495 1102 92-93 1.0046309937600952 1102 94-95 0.8966375078845985 1102 96-97 0.9122469946021745 1102 98-99 1.0255703115947803 1102 100-101 1.8043357285227266 1103 1 -0.00736869174711785 1103 2 -0.0011891939723582823 1103 3 0.013926121695206461 1103 4 0.04193823586724221 1103 5 -0.00541038219004264 1103 6 0.05972783014365746 1103 7 -0.057491741230656146 1103 8 0.044305759698573866 1103 9 0.08375735858727751 1103 10-11 0.007068985974427733 1103 12-13 0.09224666914758473 1103 14-15 0.1321256043246919 1103 16-17 -0.10599179213295429 1103 18-19 -0.05208805992273824 1103 20-21 -0.01862902036769043 1103 22-23 0.11620809785815567 1103 24-25 -0.028577773367103987 1103 26-27 -0.01442645237294471 1103 28-29 0.079982515899232 1103 30-31 0.08284202266864327 1103 32-33 0.23469020020881715 1103 34-35 0.2937592441293049 1103 36-37 0.2292723111925028 1103 38-39 0.32956731115906734 1103 40-41 0.32776161211733523 1103 42-43 0.38944156649191797 1103 44-45 0.41773552289509297 1103 46-47 0.485601870707967 1103 48-49 0.6718415883352975 1103 50-51 0.42036395929311965 1103 52-53 0.5262643744462423 1103 54-55 0.5452107633975842 1103 56-57 0.6751295436794109 1103 58-59 0.8370111786933379 1103 60-61 0.887144956904649 1103 62-63 0.5585245593432475 1103 64-65 0.5685671863807897 1103 66-67 0.9010070960637009 1103 68-69 1.0493809012978907 1103 70-71 0.6737777449864488 1103 72-73 0.594825332735919 1103 74-75 0.9524382463231582 1103 76-77 0.711195878569221 1103 78-79 0.9816926514639484 1103 80-81 0.6120284516669692 1103 82-83 0.6828883327776865 1103 84-85 0.6831781054483024 1103 86-87 1.0885945729027853 1103 88-89 1.0735006522962394 1103 90-91 1.2129816106659561 1103 92-93 1.009937942032579 1103 94-95 1.1381774622578469 1103 96-97 1.5026158280232558 1103 98-99 1.3329676256038425 1103 100-101 1.5720687397848927 1104 1 0.056425032232489514 1104 2 0.13676506850769954 1104 3 0.0516143439433705 1104 4 0.1395122602242722 1104 5 0.1625183949003386 1104 6 0.21161880714844727 1104 7 0.10772799730091975 1104 8 0.1590386494694016 1104 9 0.19362176075061655 1104 10-11 0.26194233720590177 1104 12-13 0.09933904882152689 1104 14-15 0.1954876816757718 1104 16-17 0.21166364928955517 1104 18-19 0.3668778849821308 1104 20-21 0.23391830269366665 1104 22-23 0.3966239863449843 1104 24-25 0.1839321970620631 1104 26-27 0.13704868478124865 1104 28-29 0.07112342481327971 1104 30-31 0.09804814305305598 1104 32-33 0.07323004301649405 1104 34-35 0.0628197865206559 1104 36-37 -0.12557161511386283 1104 38-39 -0.1358766404381484 1104 40-41 0.09111225995204819 1104 42-43 0.09674137972875485 1104 44-45 0.20809995424018268 1104 46-47 -0.02225288202539133 1104 48-49 -0.029772648464827967 1104 50-51 0.15751061701071478 1104 52-53 0.17556547167981762 1104 54-55 0.017204800594583958 1104 56-57 -0.08305958320281093 1104 58-59 -0.039187704618605324 1104 60-61 0.041972346889473044 1104 62-63 -0.1030729952266185 1104 64-65 0.2796022628369599 1104 66-67 0.18782959324280313 1104 68-69 0.4539164738437016 1104 70-71 0.6029074481108374 1104 72-73 0.42718350946056205 1104 74-75 0.4822936021458908 1104 76-77 0.3092951743164676 1104 78-79 0.34763050407669027 1104 80-81 0.21794602296769838 1104 82-83 0.0721934668191615 1104 84-85 0.0945519333149214 1104 86-87 0.23404367994142206 1104 88-89 0.13385944183750098 1104 90-91 0.2750305095414838 1104 92-93 0.3712322496470648 1104 94-95 0.25837220176214615 1104 96-97 0.13211224839600177 1104 98-99 0.4552537234210483 1104 100-101 0.5132152418665292 1105 1 0.013180989111241104 1105 2 -0.019571514032222126 1105 3 -0.14562383045590366 1105 4 0.030034148290361884 1105 5 0.06745706297751752 1105 6 -0.02969145429933917 1105 7 -0.1299269067341413 1105 8 0.04986278931122712 1105 9 -0.06789529128706562 1105 10-11 0.026639329800737244 1105 12-13 0.08277974450436432 1105 14-15 0.021162395795961686 1105 16-17 -0.0027052741903688116 1105 18-19 0.10214783359932511 1105 20-21 0.08260193324124998 1105 22-23 -0.01784033535165719 1105 24-25 0.06170756102356734 1105 26-27 -0.0522837166091108 1105 28-29 -0.15348427756946847 1105 30-31 -0.020116558977065324 1105 32-33 0.10347096286939461 1105 34-35 -0.1599838505757134 1105 36-37 -0.05410638666189982 1105 38-39 -0.1352406523267149 1105 40-41 -0.22797116153805774 1105 42-43 0.05675913702058466 1105 44-45 -0.047849053366491034 1105 46-47 0.03835489592030683 1105 48-49 0.0579716717205514 1105 50-51 0.24211120388290652 1105 52-53 0.26368130967286874 1105 54-55 0.45293694255216366 1105 56-57 0.29446705273492313 1105 58-59 0.3589188139446904 1105 60-61 0.33378226830401303 1105 62-63 0.10269906915301164 1105 64-65 0.20274530348982722 1105 66-67 0.23438266361709026 1105 68-69 0.21508562149369936 1105 70-71 0.36738090032987003 1105 72-73 0.7843412093245448 1105 74-75 0.6275413226637454 1105 76-77 0.3322789030958724 1105 78-79 0.2812594774281578 1105 80-81 0.3545984586132569 1105 82-83 0.39809801822715585 1105 84-85 0.5814150006666914 1105 86-87 0.6772297914723282 1105 88-89 0.726438216206553 1105 90-91 0.6408669283616959 1105 92-93 0.7734600971106715 1105 94-95 0.6140532757634567 1105 96-97 0.683831935061658 1105 98-99 0.9510410892968224 1105 100-101 1.1424483409547328 1106 1 -0.1435794820325853 1106 2 -0.07299025832509187 1106 3 -0.28429212048422414 1106 4 -0.23616487953737675 1106 5 0.019438763264375325 1106 6 -0.058063388590085196 1106 7 -0.3368593988709847 1106 8 -0.19135860585743103 1106 9 -0.3885830063133042 1106 10-11 -0.5440345497569012 1106 12-13 -0.47628617689173325 1106 14-15 -0.44575238694105934 1106 16-17 -0.4856323059289309 1106 18-19 -0.26095481635409357 1106 20-21 -0.18359478867505175 1106 22-23 -0.4726767539944916 1106 24-25 -0.5292652568924368 1106 26-27 -0.4631002859663127 1106 28-29 -0.4050638269021434 1106 30-31 -0.4491936409845039 1106 32-33 -0.24703449291705226 1106 34-35 -0.469637779387611 1106 36-37 -0.11556123230419502 1106 38-39 -0.23487447359090652 1106 40-41 -0.3430246125651095 1106 42-43 -0.6187922064034197 1106 44-45 -0.6956671999671116 1106 46-47 -0.09786682207595021 1106 48-49 -0.4478161659801714 1106 50-51 -0.7189903761276 1106 52-53 -0.3143000032208718 1106 54-55 -0.5927062683833881 1106 56-57 -0.8004327323541318 1106 58-59 -0.5850752091328673 1106 60-61 -0.8322940850354072 1106 62-63 -0.722125450986816 1106 64-65 -1.3287626703519138 1106 66-67 -1.1607168134021961 1106 68-69 -1.2014081018153782 1106 70-71 -1.3452010748216274 1106 72-73 -1.1191102789106964 1106 74-75 -1.1966744368573607 1106 76-77 -1.1846557104794861 1106 78-79 -1.1115041113079265 1106 80-81 -1.456914034814897 1106 82-83 -1.2706762514906949 1106 84-85 -1.1715527073495764 1106 86-87 -0.9937235645511713 1106 88-89 -1.3973454155117224 1106 90-91 -1.6033890462548293 1106 92-93 -1.158787613119273 1106 94-95 -1.2116982207730622 1106 96-97 -0.9668178707805986 1106 98-99 -0.9921550884243828 1106 100-101 -0.5489123312645674 1107 1 0.02509431340177315 1107 2 0.10102434526697834 1107 3 -0.06242364509461851 1107 4 0.012897883609895189 1107 5 0.1529935728755234 1107 6 0.09514744067708136 1107 7 0.17715012922305107 1107 8 0.003696244126999204 1107 9 -0.20813751600866226 1107 10-11 0.14022621548977554 1107 12-13 0.0889656946981745 1107 14-15 -0.018308938370843464 1107 16-17 0.012343262469173055 1107 18-19 0.1639566508108885 1107 20-21 0.14094005346542104 1107 22-23 0.058843242314232214 1107 24-25 0.03226938914924915 1107 26-27 -0.03568567545310941 1107 28-29 0.051967468157322116 1107 30-31 0.33202643953135436 1107 32-33 -0.018155485869030485 1107 34-35 0.23782608652695103 1107 36-37 0.2249902666980077 1107 38-39 0.3142194346579288 1107 40-41 0.20240974624952912 1107 42-43 0.224470069957448 1107 44-45 0.08370436109458268 1107 46-47 -0.3603190950916044 1107 48-49 -0.3790489352411157 1107 50-51 -0.6468289060788095 1107 52-53 -0.43742120380685634 1107 54-55 -0.1148019876622044 1107 56-57 -0.21256605645928772 1107 58-59 -0.22560882853972686 1107 60-61 -0.71946807080095 1107 62-63 -0.5909238268274493 1107 64-65 -0.313893768159069 1107 66-67 -0.1018525714393661 1107 68-69 -0.1439915303142989 1107 70-71 -0.21198655428316115 1107 72-73 -0.15598838621133382 1107 74-75 -0.16541398306170052 1107 76-77 -0.19976108473979437 1107 78-79 -0.15102090082471875 1107 80-81 -0.09468785216617803 1107 82-83 -0.04863474775904919 1107 84-85 0.082245264758253 1107 86-87 0.15781754121528735 1107 88-89 0.04101900524706892 1107 90-91 0.25566783392880765 1107 92-93 0.12842213183694895 1107 94-95 0.1829217513116994 1107 96-97 0.12915518293893413 1107 98-99 0.44379558696291355 1107 100-101 1.1280466379479215 1108 1 -2.7521196775381895E-4 1108 2 -0.045901801659169905 1108 3 -0.1008581835291622 1108 4 -0.05004817933616579 1108 5 -0.27976385988191055 1108 6 -0.22763638210674486 1108 7 -0.11106015898724308 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-0.03806049331607397 2316 68-69 0.07880929598971065 2316 70-71 0.33715740728117893 2316 72-73 0.5843674879025009 2316 74-75 0.5208878369980816 2316 76-77 0.47314459453531654 2316 78-79 0.35399482712094965 2316 80-81 0.6076549075014199 2316 82-83 0.5199774370951751 2316 84-85 0.37749636414948284 2316 86-87 0.3658724403529874 2316 88-89 0.40803570347395635 2316 90-91 0.7105145451449495 2316 92-93 0.5053372635291495 2316 94-95 0.5224380191719149 2316 96-97 0.5258632691374672 2316 98-99 0.24340903944260717 2316 100-101 0.02793943752225303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 18.0 6 38.0 7 88.0 8 206.0 9 397.0 10 569.0 11 1051.0 12 1553.0 13 2214.0 14 3047.0 15 4115.0 16 5968.0 17 8877.0 18 7938.0 19 4445.0 20 3329.0 21 3260.0 22 3235.0 23 3344.0 24 3846.0 25 4405.0 26 4944.0 27 5861.0 28 7230.0 29 8709.0 30 10858.0 31 13402.0 32 17230.0 33 22853.0 34 32328.0 35 47527.0 36 71094.0 37 103485.0 38 78819.0 39 6339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30031662086936 20.060034078885106 15.567023499808078 20.07262580043746 2 15.8245911755631 21.611060588837102 39.578427710745544 22.985920524854254 3 19.29959888923172 26.964549602949056 30.371149255427987 23.364702252391236 4 12.665869585957848 17.8405337869521 36.59351522742174 32.90008139966831 5 12.774816180202272 39.08814824137945 32.658819899228995 15.478215679189283 6 30.101576836259113 37.3069552932006 16.93941928254762 15.65204858799266 7 27.52764741226717 35.48713847253122 19.60367982591468 17.381534289286932 8 27.644773380535568 34.112278536514076 18.768167719514768 19.47478036343559 9 27.839036035011937 16.61808408710762 19.949982948732522 35.59289692914792 10-11 25.07967407720164 28.10799948034233 25.473375663781482 21.338950778674548 12-13 26.680369259501735 25.740980395142177 27.10890516283248 20.46974518252361 14-15 23.155925721431007 26.395971029084592 27.079848649702008 23.368254599782393 16-17 20.76753032689716 29.99135257149353 27.494458338069794 21.746658763539518 18-19 20.754031406810764 26.72187352831322 32.36442212442554 20.15967294045048 20-21 21.86664880341594 25.572258371622603 32.7741932864626 19.786899538498858 22-23 21.486309003030716 25.838927536879115 32.74847094025248 19.926292519837684 24-25 21.08287727959207 26.189325906559052 32.89057552087563 19.837221292973254 26-27 20.70142532207529 26.405860029576218 32.80747748543264 20.085237162915845 28-29 20.37409756498598 26.553741127895332 33.01818521371712 20.05397609340158 30-31 20.520713758661394 26.303941181187156 32.907152709479604 20.26819235067185 32-33 20.384891765049773 26.600890725734423 32.71782994202569 20.296387567190113 34-35 20.681056041832445 26.36501485896166 32.878294548466194 20.0756345507397 36-37 21.148898206254817 26.18870000416135 32.359171261914405 20.30323052766943 38-39 20.76813930073564 26.635196251969017 32.39365286867276 20.203011578622583 40-41 20.692546051155887 26.646184825777482 32.496094951297266 20.165174171769365 42-43 20.791728422634154 26.856130591029988 31.742228899762452 20.609912086573406 44-45 20.67293639065266 27.064015329901427 31.5299249744231 20.733123305022815 46-47 21.059330291170692 27.615948618847337 30.73602608031959 20.588695009662388 48-49 21.335195440003897 27.795798892479578 29.999776709592556 20.86922895792397 50-51 21.502764741226716 28.106375550106367 29.5631424673996 20.827717241267315 52-53 21.521541434579973 28.517432891083 29.122346903977004 20.838678770360026 54-55 21.179196641712274 28.48261988664967 29.199483590184965 21.138699881453093 56-57 21.3124604167005 28.715653875509506 29.083068091394793 20.8888176163952 58-59 21.13880137709284 28.250905341106545 29.258960035076893 21.351333246723723 60-61 21.19695837866805 28.259735461764564 29.76095746926712 20.782348690300264 62-63 21.129565273875833 28.41380584290099 29.906197729745532 20.550431153477646 64-65 20.914597508891017 28.3698582308904 29.7201562220887 20.99538803812988 66-67 20.999447863719777 28.66450007307686 29.41526332028776 20.920788742915605 68-69 21.071712759219864 28.562395459491057 29.333457834651423 21.032433946637653 70-71 21.074859124052033 28.927170788742917 29.054750807905293 20.94321927929976 72-73 21.05293606586661 28.858458240633983 28.695862225758784 21.392743467740626 74-75 21.067348446710728 29.165787037788853 28.380515273063867 21.386349242436545 76-77 21.029592068724728 28.773709381444974 28.962389775735236 21.234308774095066 78-79 21.033854885594113 28.870637717403657 28.6800289059582 21.415478491044023 80-81 21.01660062683707 29.417090241803212 28.365595414021012 21.200713717338704 82-83 21.029714908608156 29.341201283312174 28.200084647449465 21.42899916063021 84-85 20.77165339945456 29.32979180136025 28.119351116100198 21.779203683084987 86-87 20.872375322756135 29.212779518991866 28.008127770830963 21.906717387421036 88-89 20.642487698728463 29.22922181263093 28.089730265187807 22.038560223452798 90-91 20.802212860985637 29.197482616860377 28.138151550525304 21.86215297162869 92-93 20.84690454561602 28.983541997594482 28.172263470136567 21.99728998665293 94-95 20.571237759625845 28.924633397749233 28.188282531382452 22.315846311242467 96-97 20.94362526185875 28.997811754007046 27.925307328797157 22.13325565533705 98-99 20.77733717258676 29.198862027422173 27.402485229802053 22.621315570189015 100-101 20.455560563426033 29.259904417347805 27.796929498658024 22.487605520568135 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1123.0 1 716.0 2 409.5 3 523.5 4 444.0 5 415.5 6 713.0 7 1383.0 8 2049.5 9 2292.0 10 2073.5 11 1830.0 12 1827.0 13 1892.5 14 1944.0 15 1989.5 16 1955.0 17 1900.0 18 1915.5 19 1914.0 20 2002.5 21 2156.5 22 2341.5 23 2662.0 24 3014.0 25 3414.0 26 3958.5 27 4505.0 28 5048.5 29 5813.5 30 6724.0 31 7398.5 32 8281.5 33 9113.0 34 9862.0 35 10711.0 36 11742.0 37 12678.5 38 13159.0 39 13417.0 40 13604.0 41 14325.5 42 14630.5 43 15167.5 44 16512.0 45 17429.0 46 16957.5 47 16722.5 48 17976.0 49 17575.5 50 16602.5 51 15714.5 52 14526.0 53 14182.5 54 14040.5 55 13366.5 56 12009.0 57 10941.5 58 10379.5 59 9348.5 60 7641.5 61 6302.0 62 5173.0 63 3814.0 64 2717.0 65 2081.5 66 1563.0 67 1253.0 68 924.0 69 580.5 70 421.5 71 315.0 72 269.0 73 233.0 74 183.5 75 133.5 76 87.0 77 59.0 78 42.0 79 25.0 80 22.0 81 16.0 82 5.0 83 2.5 84 2.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04973286347618506 2 0.0 3 0.0 4 2.0299127949463292E-4 5 0.006089738384838987 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004973286347618506 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0149563974731646E-4 22-23 0.001826921515451696 24-25 0.0 26-27 0.001319443316715114 28-29 0.001319443316715114 30-31 1.0149563974731646E-4 32-33 0.0 34-35 0.0 36-37 7.104694782312152E-4 38-39 0.0 40-41 1.0149563974731646E-4 42-43 0.020705110508452557 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0149563974731646E-4 84-85 3.044869192419494E-4 86-87 0.0 88-89 0.0 90-91 0.011570502931194077 92-93 0.002943373552672177 94-95 0.0 96-97 0.0 98-99 3.044869192419494E-4 100-101 0.015021354682602836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 492632.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.65815947746741 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97284047050135 46.11086789714226 2 11.362290491415333 13.102575143666737 3 3.550853402257185 6.142070152453575 4 1.5878783241524939 3.6621656657919277 5 0.8405869214584626 2.4233347386062705 6 0.5378677425759771 1.8607478447538646 7 0.3672779283751372 1.4823598556765256 8 0.27999028436261025 1.2914979574336665 9 0.2091510966663606 1.0853340557828597 >10 1.172142607996175 12.396608491213483 >50 0.08200596277270643 3.1960949356224155 >100 0.034286975659253954 3.526669211170399 >500 0.0010604219276057923 0.4045560786045562 >1k 0.0014138959034743898 2.167686877601038 >5k 3.5347397586859745E-4 1.147431094480429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5630 1.1428409035547833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4543 0.9221893827441173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.6861105246918593 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1567 0.3180873349680898 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1146 0.2326280063008493 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 903 0.1833011253836535 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 553 0.11225417756053199 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.1073823868526608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.0299127949463292E-4 0.0 24-25 0.0 0.0 0.0 8.119651179785317E-4 0.0 26-27 0.0 0.0 0.0 0.001116452037220481 0.0 28-29 0.0 0.0 0.0 0.003755338670650709 0.0 30-31 0.0 0.0 0.0 0.009540590136247747 0.0 32-33 0.0 0.0 0.0 0.016645284918559898 0.0 34-35 0.0 0.0 0.0 0.021821562545673037 0.0 36-37 0.0 0.0 0.0 0.02740382273177544 0.0 38-39 0.0 0.0 0.0 0.0326815959986359 0.0 40-41 0.0 0.0 0.0 0.039075821302716834 0.0 42-43 0.0 0.0 0.0 0.0536911934263304 0.0 44-45 0.0 0.0 0.0 0.07307686061806784 0.0 46-47 0.0 0.0 0.0 0.09611637084070868 0.0 48-49 0.0 0.0 0.0 0.14503726919891521 0.0 50-51 0.0 0.0 0.0 0.18979684632748178 0.0 52-53 0.0 0.0 0.0 0.24145812695886587 0.0 54-55 0.0 0.0 0.0 0.3293533509800419 0.0 56-57 0.0 0.0 0.0 0.415015670926777 0.0 58-59 0.0 0.0 0.0 0.5180337452703032 0.0 60-61 0.0 0.0 0.0 0.6279535231166469 0.0 62-63 0.0 0.0 0.0 0.777761087383686 0.0 64-65 0.0 0.0 0.0 0.8916391951801751 0.0 66-67 0.0 0.0 0.0 1.044288637360139 0.0 68-69 0.0 0.0 0.0 1.2457574822585622 0.0 70-71 0.0 0.0 0.0 1.3813556569609768 0.0 72-73 1.0149563974731646E-4 0.0 0.0 1.6053565338833045 0.0 74-75 2.0299127949463292E-4 0.0 0.0 1.8894428295360433 0.0 76-77 2.0299127949463292E-4 0.0 0.0 2.1064405073158055 0.0 78-79 2.0299127949463292E-4 0.0 0.0 2.3180589161889604 0.0 80-81 2.0299127949463292E-4 0.0 0.0 2.6035661507981613 0.0 82-83 2.0299127949463292E-4 0.0 0.0 2.946519917504344 0.0 84-85 3.0448691924194935E-4 0.0 0.0 3.303480082495656 0.0 86-87 4.0598255898926584E-4 0.0 0.0 3.7732019032462367 0.0 88-89 4.0598255898926584E-4 0.0 0.0 4.349291154452005 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2770 0.0 45.112003 1 GTATCAA 4725 0.0 38.211834 1 GTACCGG 65 0.0058328244 29.239016 1 TATCAAC 6185 0.0 29.029259 2 ATCAACG 6180 0.0 28.97589 3 TCAACGC 6215 0.0 28.889135 4 CAACGCA 6305 0.0 28.552095 5 AGGTAAG 150 3.3292963E-8 28.499361 2 AACGCAG 6410 0.0 28.084393 6 CGCGTAT 85 6.402781E-4 27.94055 7 CTAGTAC 70 0.008387916 27.142248 3 GGTAAGG 180 6.681148E-9 26.388296 3 ACGCGTA 90 8.9414313E-4 26.388296 6 TACGCGT 95 0.00122572 24.99944 5 ACGCAGA 7290 0.0 24.694233 7 CGCAGAG 7280 0.0 24.662907 8 GAGTACT 4255 0.0 24.056452 12-13 AGTACTT 4375 0.0 23.613754 12-13 GCAGAGT 7665 0.0 23.362162 9 CTAGATC 145 2.3312452E-5 22.930521 4 >>END_MODULE