FastQCFastQC Report
Thu 26 May 2016
SRR937800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362728
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56381.5543327231424098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42121.1612006792968836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.7978430118435853No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.2809267550340751No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.23405968108334618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.18388434308903642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.15273152334531662No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.13012505238084734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4600.12681678833726648No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.11496217551443506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.10062636465891799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.052.3461951
GTATCAA33850.043.980241
TATGACG500.0016081938.029594
ATCAACG42300.034.94073
TATCAAC43000.034.592942
TGACGAC550.002574452434.553246
TCAACGC43150.034.2618874
CAACGCA44450.033.255255
AACGCAG45700.032.3322336
GTCCTAC600.003921751431.7088641
ACGCAGA50200.029.2446387
CGCAGAG50650.029.0786198
GTCTTAT1004.8991704E-528.5379771
GTGGTAT8100.028.1856561
GCAGAGT51500.027.85689
GTACATG24250.027.8514981
AGGCGAC1056.863002E-527.1602385
GCGACAG1056.8824105E-527.1489777
TACGGGC350.008391635527.14147820-21
TGGTATC8400.026.5907232
Produced by FastQC (version 0.11.5)