FastQCFastQC Report
Thu 26 May 2016
SRR937800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362728
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39231.0815266535806445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.9202487814560774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23360.644008733817075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13940.3843100063959771No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.2544606426854282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.18553847511082686No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4110.11330804349264463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.046.2218131
GTATCAA34000.038.149561
AGGTAAG1502.5465852E-1134.8327262
TAGGTAG702.0507813E-433.9279826
TAAGGTG1753.6379788E-1232.570865
GTAAGGT1753.6379788E-1232.570864
TGTGGCG600.003947755331.6661158
TATCAAC43150.029.8314632
CTAGACT804.4881026E-429.6869834
TAGGCCC804.4881026E-429.6869834
GGTAAGG1951.6370905E-1129.2302593
ATCAACG44150.029.1557773
TCAACGC44900.028.6687644
CAACGCA45500.028.2907165
AACGCAG45650.028.1977586
AGGACCG700.00838484327.1423855
ACGGCGA350.00839027727.14238584-85
TACCTGG2650.026.8863222
GGTGTGC3700.025.6752288
ACGCAGA52700.024.4255717
Produced by FastQC (version 0.11.5)