##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 362728 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26952978540394 33.0 31.0 34.0 31.0 34.0 2 32.478165457312365 34.0 31.0 34.0 31.0 34.0 3 32.55144074899098 34.0 31.0 34.0 31.0 34.0 4 36.16461921880858 37.0 35.0 37.0 35.0 37.0 5 36.03079166758563 37.0 35.0 37.0 35.0 37.0 6 36.15050947266271 37.0 35.0 37.0 35.0 37.0 7 36.11017070642465 37.0 35.0 37.0 35.0 37.0 8 36.114829844952695 37.0 35.0 37.0 35.0 37.0 9 37.772452085309105 39.0 38.0 39.0 35.0 39.0 10-11 37.7312931452769 39.0 37.0 39.0 35.0 39.0 12-13 37.65298377847864 39.0 37.0 39.0 35.0 39.0 14-15 38.825756489711296 40.0 38.0 41.0 35.0 41.0 16-17 38.80990301272579 40.0 38.0 41.0 35.0 41.0 18-19 38.85791557310161 40.0 38.0 41.0 35.0 41.0 20-21 38.8205390816259 40.0 38.0 41.0 35.0 41.0 22-23 38.66810943737456 40.0 38.0 41.0 34.5 41.0 24-25 38.57876838843431 40.0 38.0 41.0 34.0 41.0 26-27 38.468991916808186 40.0 38.0 41.0 34.0 41.0 28-29 38.33580258485698 40.0 38.0 41.0 34.0 41.0 30-31 38.30016568889085 40.0 37.5 41.0 34.0 41.0 32-33 38.10997634591209 40.0 37.0 41.0 34.0 41.0 34-35 37.93962418120465 40.0 37.0 41.0 33.5 41.0 36-37 37.7364140623277 40.0 36.5 41.0 33.0 41.0 38-39 37.553701396087426 40.0 36.0 41.0 33.0 41.0 40-41 37.312327694581064 40.0 36.0 41.0 33.0 41.0 42-43 37.02663290399418 40.0 35.5 41.0 32.5 41.0 44-45 36.586646192188084 40.0 35.0 41.0 31.0 41.0 46-47 36.015517136807745 39.0 35.0 41.0 29.5 41.0 48-49 35.50256528307713 39.0 35.0 41.0 27.5 41.0 50-51 35.162314461524886 39.0 35.0 41.0 26.0 41.0 52-53 34.89891599214839 39.0 35.0 40.0 24.5 41.0 54-55 34.66955680289363 38.5 35.0 40.0 24.0 41.0 56-57 34.364485234114824 38.0 34.0 40.0 23.0 41.0 58-59 34.081800963807595 38.0 34.0 40.0 22.5 41.0 60-61 33.72540029994927 37.5 34.0 40.0 21.5 41.0 62-63 33.35178150018747 37.0 33.0 40.0 19.5 41.0 64-65 32.936048499150886 36.0 33.0 39.5 17.5 41.0 66-67 32.50545863567191 36.0 32.5 39.0 13.0 40.5 68-69 32.04223274740301 35.0 32.0 39.0 8.0 40.0 70-71 31.62667067334201 35.0 31.5 38.0 6.5 40.0 72-73 31.190806885600228 35.0 31.0 37.0 2.0 39.5 74-75 30.71933928453276 35.0 31.0 37.0 2.0 39.0 76-77 29.523499702256238 33.5 29.5 35.5 2.0 38.0 78-79 30.026909971107827 34.0 30.0 36.0 2.0 38.0 80-81 29.831359861935113 34.0 30.0 35.5 2.0 37.0 82-83 29.49077821397852 34.0 30.0 35.0 2.0 37.0 84-85 29.110008050109172 34.0 29.5 35.0 2.0 36.5 86-87 28.695212390551596 34.0 29.0 35.0 2.0 36.0 88-89 28.291606934121432 34.0 29.0 35.0 2.0 36.0 90-91 27.801642001720296 34.0 27.5 35.0 2.0 35.0 92-93 27.369720561963785 33.0 26.5 35.0 2.0 35.0 94-95 26.9322315895106 33.0 26.0 35.0 2.0 35.0 96-97 26.44481677730972 33.0 25.0 35.0 2.0 35.0 98-99 25.701594583269006 33.0 23.5 35.0 2.0 35.0 100-101 23.47601784257074 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15749390076522474 1101 2 0.24313087898562458 1101 3 0.22527695569193185 1101 4 0.13345758228994953 1101 5 -0.007015913720749722 1101 6 0.10138506436863537 1101 7 0.034488791266049645 1101 8 0.09376843673454971 1101 9 0.3329916129330428 1101 10-11 0.27225302169537713 1101 12-13 0.2695579326123152 1101 14-15 0.1877176110294343 1101 16-17 -0.07159848541400748 1101 18-19 0.18531322414392548 1101 20-21 0.27487453821722596 1101 22-23 0.23495167357361169 1101 24-25 0.30481238136383837 1101 26-27 0.31077783498409417 1101 28-29 0.18973004083704126 1101 30-31 0.3382644368371146 1101 32-33 0.4669391097800073 1101 34-35 0.29037332041509956 1101 36-37 0.42656499130506376 1101 38-39 0.4895406991790523 1101 40-41 0.42903200041467215 1101 42-43 0.4902690165664225 1101 44-45 0.5011040810940202 1101 46-47 0.44975705191578186 1101 48-49 0.33356320609431833 1101 50-51 0.694319257105029 1101 52-53 0.691111053234188 1101 54-55 0.8051153228294368 1101 56-57 0.7526175403370132 1101 58-59 0.7393978931441225 1101 60-61 0.8053299868252779 1101 62-63 0.7600662855713196 1101 64-65 0.728646788152794 1101 66-67 0.9271613990536736 1101 68-69 0.6805399987357212 1101 70-71 0.8273139489714971 1101 72-73 0.7303679353101664 1101 74-75 0.6178551704155701 1101 76-77 0.739851723211352 1101 78-79 0.828917681664489 1101 80-81 0.8498233228849159 1101 82-83 0.7324386578685171 1101 84-85 0.9371030532472773 1101 86-87 1.3951721715971246 1101 88-89 1.4482730459762827 1101 90-91 1.5934631860667565 1101 92-93 1.630937386347064 1101 94-95 1.7578894853659932 1101 96-97 1.6891960959464782 1101 98-99 1.6057697860328446 1101 100-101 2.014501257937191 1102 1 0.15524143005273316 1102 2 0.17212955336889024 1102 3 0.14920487662913473 1102 4 0.121640194078239 1102 5 -0.03349052762141014 1102 6 -0.07715287736888854 1102 7 -0.01765482799095963 1102 8 0.028791661970842597 1102 9 0.10785500974510143 1102 10-11 0.03536369452669419 1102 12-13 0.04037712064512533 1102 14-15 0.0454360952998627 1102 16-17 0.27106131606680606 1102 18-19 0.2956964135993445 1102 20-21 0.13882099623397437 1102 22-23 0.23264801034492422 1102 24-25 0.2203447296453902 1102 26-27 -0.022981204536527855 1102 28-29 0.0732778438697963 1102 30-31 0.2663093138134158 1102 32-33 0.23895728042891307 1102 34-35 0.1662206912752353 1102 36-37 0.3162868812249897 1102 38-39 0.12380115592407748 1102 40-41 0.1988246437806538 1102 42-43 0.3755950859511472 1102 44-45 0.31068947559972315 1102 46-47 0.36647625827012575 1102 48-49 0.47058267002784504 1102 50-51 0.4619712449912683 1102 52-53 0.19676782627351486 1102 54-55 0.08989929041111111 1102 56-57 0.3385980656261154 1102 58-59 0.18789552751101013 1102 60-61 0.23070207558771472 1102 62-63 0.15508929526018278 1102 64-65 0.35368989764010905 1102 66-67 0.4975376371042586 1102 68-69 0.5368008434661213 1102 70-71 0.5388804938538634 1102 72-73 0.5850593252678102 1102 74-75 0.6474148070025905 1102 76-77 0.809414269688773 1102 78-79 0.7958729124619062 1102 80-81 0.9071387334488961 1102 82-83 0.7593969063300463 1102 84-85 0.7813538642445081 1102 86-87 0.6838373402091058 1102 88-89 0.46075458972702066 1102 90-91 0.7170877239869391 1102 92-93 1.1266318263009367 1102 94-95 0.901158477789501 1102 96-97 1.220236273988661 1102 98-99 1.3500240996759203 1102 100-101 1.8816957463892336 1103 1 0.12203299357514652 1103 2 0.25379090630043777 1103 3 0.1704116563772189 1103 4 0.10753872902848371 1103 5 -0.2917123688386525 1103 6 -0.05960310324276463 1103 7 -0.05390060188591406 1103 8 0.12035807402772036 1103 9 0.12105105285859707 1103 10-11 0.12380126375776968 1103 12-13 0.15088354689336825 1103 14-15 0.05348375516943804 1103 16-17 0.10867774465886981 1103 18-19 0.07337840501388371 1103 20-21 0.08336871871251361 1103 22-23 0.16448611319700035 1103 24-25 0.08129022131839037 1103 26-27 0.4085310106328208 1103 28-29 0.22290363490996157 1103 30-31 0.275180522359058 1103 32-33 0.1891831543055602 1103 34-35 0.2628439811675847 1103 36-37 0.3442474022599953 1103 38-39 0.3071795998184186 1103 40-41 0.09558888410917632 1103 42-43 -0.08378614484475833 1103 44-45 -0.0627473070058997 1103 46-47 -0.2844659263653142 1103 48-49 -0.22034577274275335 1103 50-51 -0.005772295850640319 1103 52-53 -0.38252562354460196 1103 54-55 -0.14426465517367149 1103 56-57 0.01064428709489107 1103 58-59 -0.060167608010104345 1103 60-61 -0.2600382192202346 1103 62-63 -0.20594128038911208 1103 64-65 -0.12786132772968273 1103 66-67 0.09713565545028757 1103 68-69 0.2652143965408662 1103 70-71 0.5085600964956392 1103 72-73 0.496067907511911 1103 74-75 0.7194729081208813 1103 76-77 0.6665791431705017 1103 78-79 0.8003020953025519 1103 80-81 0.641362077281272 1103 82-83 0.5280776551974711 1103 84-85 0.7074592335250216 1103 86-87 0.577551400255917 1103 88-89 0.7729582004983335 1103 90-91 0.9333663756146109 1103 92-93 1.0758061906594207 1103 94-95 1.1616470845634694 1103 96-97 1.1179011201793045 1103 98-99 0.8331549973600296 1103 100-101 1.8930697081239174 1104 1 0.03622891387875171 1104 2 0.06955229149967579 1104 3 0.03983765258215044 1104 4 0.04290400417080065 1104 5 -0.07171711267167069 1104 6 -0.1242378281004548 1104 7 -0.06570088651589145 1104 8 -0.05148727701592293 1104 9 -0.046762040120533754 1104 10-11 -0.237441620492703 1104 12-13 -0.05247864475747832 1104 14-15 -0.06265120687989167 1104 16-17 -0.0476019042974869 1104 18-19 -0.16271623445480543 1104 20-21 -0.08563033252087848 1104 22-23 0.014047308642929579 1104 24-25 -0.31891139158482673 1104 26-27 -0.15112268955692798 1104 28-29 -0.41523085275606775 1104 30-31 -0.39773219111342684 1104 32-33 -0.31579787036236695 1104 34-35 -0.42628671143203434 1104 36-37 -0.416248328290294 1104 38-39 -0.48592998272427934 1104 40-41 -0.6464058976745051 1104 42-43 -0.5939853858314805 1104 44-45 -0.9134648117497406 1104 46-47 -0.7738812489459619 1104 48-49 -0.4940056399154287 1104 50-51 -0.6037324476533072 1104 52-53 -0.3814582611157533 1104 54-55 -0.5747378525171314 1104 56-57 -0.28949209810435406 1104 58-59 -0.6325110615205389 1104 60-61 -0.5174208093530623 1104 62-63 -0.412031176024783 1104 64-65 -0.3644007015437225 1104 66-67 -0.17690964815501786 1104 68-69 -0.20709347822194246 1104 70-71 -0.171468366502463 1104 72-73 -0.0884019691484319 1104 74-75 0.01270108648900603 1104 76-77 -0.35725861774031387 1104 78-79 -0.305396434109209 1104 80-81 -0.4126286722443453 1104 82-83 0.1825487178160614 1104 84-85 0.16556525819294876 1104 86-87 0.13853930348172483 1104 88-89 0.1326000411055439 1104 90-91 -0.12954870977438304 1104 92-93 0.00838446390419989 1104 94-95 0.20656525344392662 1104 96-97 0.5463581884905011 1104 98-99 0.6212835552347933 1104 100-101 1.1699316625831244 1105 1 0.25953116902398676 1105 2 0.18153868034801945 1105 3 0.07480517557149824 1105 4 0.14067257810161493 1105 5 0.03305773771513287 1105 6 0.17233384026913257 1105 7 0.1943843200232962 1105 8 0.19830289960063396 1105 9 0.2705182839197562 1105 10-11 0.23480695635738869 1105 12-13 0.17920058090300728 1105 14-15 0.25090019072629843 1105 16-17 0.22289464730081932 1105 18-19 0.21503655710846914 1105 20-21 0.34500935511596253 1105 22-23 0.2967295813038504 1105 24-25 0.23170621898297128 1105 26-27 0.35211588875280597 1105 28-29 0.11919614695199954 1105 30-31 -0.13308104529529174 1105 32-33 0.022711106429333938 1105 34-35 -0.13724359728811208 1105 36-37 -0.04226551556222091 1105 38-39 -0.33097541404817576 1105 40-41 -0.38609262224026253 1105 42-43 -0.3956384291828101 1105 44-45 -0.796654765771045 1105 46-47 -1.2276129122068014 1105 48-49 -1.4064499911050348 1105 50-51 -1.3456496860126705 1105 52-53 -1.1710236530293514 1105 54-55 -1.2134516351765967 1105 56-57 -1.4895016769979463 1105 58-59 -1.4093867001134512 1105 60-61 -1.4700805488071573 1105 62-63 -1.4132832742586174 1105 64-65 -1.6489531391440764 1105 66-67 -1.9431504341716597 1105 68-69 -1.9265094393954918 1105 70-71 -1.703598712437362 1105 72-73 -1.6158135696447111 1105 74-75 -1.3504438923462025 1105 76-77 -1.3101395840226076 1105 78-79 -1.3824449489245687 1105 80-81 -1.2946326680113884 1105 82-83 -0.8937585730903734 1105 84-85 -0.8512913138403277 1105 86-87 -0.8101292303089629 1105 88-89 -0.6510674891820045 1105 90-91 -0.5726911342379672 1105 92-93 -0.5813241552754818 1105 94-95 -0.8468188328208548 1105 96-97 -0.6565843563934592 1105 98-99 -0.4086345224181045 1105 100-101 0.1216494775006538 1106 1 -0.33355447244875336 1106 2 -0.230874974001118 1106 3 -0.14521136702568782 1106 4 -0.1822880996977716 1106 5 -0.3408943781724645 1106 6 -0.38467105544544467 1106 7 -0.3398297948359783 1106 8 -0.46295389068841786 1106 9 -0.06189850010993325 1106 10-11 -0.1308570947905352 1106 12-13 -0.5269503591188993 1106 14-15 0.04145749741263671 1106 16-17 -0.49759693609398425 1106 18-19 -0.2895461894097622 1106 20-21 -0.31545942632056523 1106 22-23 -0.1156393633231545 1106 24-25 -0.474366479025214 1106 26-27 -1.1328728220423585 1106 28-29 -0.41189387607349914 1106 30-31 -0.12501094045099848 1106 32-33 -0.3532250033777373 1106 34-35 -0.4878013510716741 1106 36-37 -0.6588125837221952 1106 38-39 -0.013106055856233922 1106 40-41 -0.08531355533216356 1106 42-43 0.2521327911690534 1106 44-45 -0.0542554185329891 1106 46-47 0.428647566634325 1106 48-49 0.4985586155164796 1106 50-51 0.27948164879608584 1106 52-53 0.7358907054979156 1106 54-55 0.09861104785382224 1106 56-57 0.23961329405250353 1106 58-59 0.06654829195057488 1106 60-61 0.4770893257820745 1106 62-63 0.45713118486551707 1106 64-65 0.40476000327874573 1106 66-67 0.8863769840654356 1106 68-69 0.5102654247987282 1106 70-71 0.4840032816162463 1106 72-73 0.8036306885092266 1106 74-75 0.7966007950210674 1106 76-77 0.7976499673153299 1106 78-79 1.3004345807291564 1106 80-81 1.2879294226152211 1106 82-83 1.4234569054154278 1106 84-85 1.672628387498726 1106 86-87 1.3428897936778057 1106 88-89 1.791698477777512 1106 90-91 1.0782712425308638 1106 92-93 1.3123225932099771 1106 94-95 1.464953237015731 1106 96-97 1.7924912701015216 1106 98-99 1.8912391726168813 1106 100-101 1.9218361405905426 1107 1 0.43763979874551495 1107 2 0.26866302553688115 1107 3 0.33769461588029515 1107 4 0.17044925303957825 1107 5 -0.05930509658318783 1107 6 0.4062737354993473 1107 7 0.12228616727998087 1107 8 0.46243303469850616 1107 9 0.5624951242836929 1107 10-11 0.2822290682956279 1107 12-13 0.25729542512688397 1107 14-15 0.4251488811040218 1107 16-17 0.4005897120926605 1107 18-19 0.4517335518699781 1107 20-21 0.6043834935223558 1107 22-23 0.4824202347364377 1107 24-25 0.331651076992344 1107 26-27 -0.1258273149968545 1107 28-29 0.23196151778189034 1107 30-31 0.3995905841505234 1107 32-33 0.37644697629423973 1107 34-35 0.6603852971149777 1107 36-37 0.41637799146838006 1107 38-39 0.28199673924655855 1107 40-41 0.4975577775391713 1107 42-43 0.35642939546566055 1107 44-45 0.8509189866414104 1107 46-47 0.61633525432201 1107 48-49 0.8400347069925687 1107 50-51 0.8960790153934539 1107 52-53 0.6224695920767971 1107 54-55 0.6468323460751293 1107 56-57 0.9426047470439585 1107 58-59 1.0340927594950458 1107 60-61 1.2393900880828355 1107 62-63 0.9873922151265475 1107 64-65 1.1363377348564754 1107 66-67 1.525901123589577 1107 68-69 1.3342432487765485 1107 70-71 1.429775977388939 1107 72-73 1.4322978171763516 1107 74-75 1.5189385173587873 1107 76-77 1.3406737603391221 1107 78-79 1.2121924924870662 1107 80-81 1.4356540703398686 1107 82-83 1.160694142652666 1107 84-85 1.0227092375795763 1107 86-87 1.6185308193188312 1107 88-89 1.6594508455298786 1107 90-91 1.8261922404518636 1107 92-93 1.6859077167951 1107 94-95 1.6633824354449303 1107 96-97 1.764424742034997 1107 98-99 2.7194327508104585 1107 100-101 2.6619031306575316 1108 1 0.09759095869667789 1108 2 -0.0684554115815601 1108 3 -0.08806850988283088 1108 4 -0.06105257846225243 1108 5 -0.12133195861004964 1108 6 -0.10410477487916125 1108 7 -0.05475900976519199 1108 8 0.04875782102329396 1108 9 0.1709200327086009 1108 10-11 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-0.07002341850012073 2316 98-99 0.034371435314358934 2316 100-101 -0.9421474081756145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 5.0 5 10.0 6 18.0 7 52.0 8 111.0 9 225.0 10 387.0 11 637.0 12 1050.0 13 1526.0 14 1938.0 15 2878.0 16 4142.0 17 6149.0 18 5588.0 19 3091.0 20 2421.0 21 2518.0 22 2417.0 23 2392.0 24 2741.0 25 3009.0 26 3491.0 27 4107.0 28 4781.0 29 6008.0 30 7673.0 31 9412.0 32 11932.0 33 16194.0 34 22994.0 35 34515.0 36 52480.0 37 78314.0 38 62286.0 39 5233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39032328353022 20.19494650555355 15.408441613962893 20.00628859695334 2 15.803301647515495 21.76038243532344 39.6787675613683 22.75754835579277 3 19.375399748571933 26.987439624181203 30.513222028627514 23.12393859861935 4 12.524811980326856 17.93079111620829 36.66273350830374 32.88166339516111 5 12.76751200736709 39.117823840482615 32.87647438336449 15.238189768785809 6 30.146556097130635 37.33265697712887 16.937209148452837 15.583577777287664 7 27.30613572704616 35.72677047264066 19.74757945347478 17.2195143468384 8 27.66039566837961 34.105445402615736 18.884122538100172 19.350036390904478 9 27.948766017511744 16.80653271873139 19.99321805871066 35.251483205046206 10-11 24.96195496349882 28.261396969630137 25.442342471493788 21.334305595377252 12-13 26.47354795664892 25.977149978081844 27.173688287985264 20.37561377728397 14-15 23.131795725722856 26.475072230431618 27.074281555325204 23.318850488520322 16-17 20.704081295075095 30.16061621931585 27.482438631701996 21.65286385390706 18-19 20.6346077501599 27.013216514854104 32.34930857281489 20.002867162171103 20-21 21.778164354557685 25.936238724333382 32.61645089433405 19.669146026774882 22-23 21.357276074397653 26.231196231871674 32.557863845073356 19.85366384865732 24-25 21.07210401239503 26.48321322430705 32.59333491395709 19.851347849340826 26-27 20.62431076312307 26.778231142479047 32.50234340538156 20.09511468901632 28-29 20.241922143802384 26.94033965593295 32.8586788707543 19.959059329510367 30-31 20.4510836677221 26.68112200239023 32.70811479172324 20.15967953816443 32-33 20.279520742815553 27.032376877439845 32.49142608235372 20.196676297390884 34-35 20.54211420127478 26.90721422112437 32.610385743587486 19.940285834013366 36-37 20.99396793189387 26.589345184270307 32.279559339229394 20.137127544606425 38-39 20.663417216206085 27.005635076420898 32.17907081890562 20.151876888467392 40-41 20.598216845680508 27.156988156414723 32.27694029686156 19.967854701043205 42-43 20.716894202892558 27.435450984933397 31.471410118021065 20.376244694152987 44-45 20.531224498797997 27.654744050638495 31.340839416863325 20.473192033700183 46-47 20.950409121986723 28.129204252222053 30.479863699521403 20.44052292626982 48-49 21.274343309587348 28.239204031671118 29.807321188328444 20.679131470413093 50-51 21.302601398292936 28.54135881431817 29.41198363512053 20.744056152268367 52-53 21.258215522374893 29.023951831675525 29.069026929269313 20.64880571668027 54-55 20.905334024392932 28.939591098564215 29.18398910478375 20.971085772259105 56-57 21.117752144857853 29.204114377715534 28.871771685670804 20.806361791755805 58-59 21.008579431419687 28.64239871198253 29.199289825985314 21.14973203061247 60-61 21.064406387155113 28.768526278644053 29.62109349154187 20.545973842658963 62-63 20.89223881255376 28.964127390220774 29.70421362563684 20.43942017158863 64-65 20.669758055622946 28.79375179197636 29.60951456738934 20.92697558501136 66-67 20.79188813656514 29.028776383405745 29.31025451578042 20.869080964248692 68-69 20.892652345559206 28.925117443373548 29.200806113671952 20.981424097395294 70-71 20.884381685450254 29.334790807436978 28.925806665049294 20.855020842063475 72-73 20.88298054178636 29.163886790580218 28.582869714329824 21.370262953303595 74-75 20.80534507404339 29.531478951772204 28.392880034957468 21.270295939226937 76-77 20.798504664652302 29.11134514016012 28.869704020643567 21.220446174544012 78-79 20.76983304294127 29.112172206171017 28.70043117708035 21.41756357380737 80-81 20.719657704946957 29.78044154297435 28.42460466244679 21.0752960896319 82-83 20.799773934978738 29.700532769089744 28.175145253668386 21.32454804226313 84-85 20.462440175558545 29.74322357248407 28.125482455173024 21.66885379678437 86-87 20.547214441675305 29.70531638031804 27.76901700447719 21.97845217352948 88-89 20.296062063033457 29.63418870338105 27.978953926909423 22.09079530667608 90-91 20.46071768290161 29.614827885689078 28.001350997394503 21.923103434014806 92-93 20.57653842601591 29.416775911765804 27.960817883939658 22.045867778278627 94-95 20.22603714077766 29.428524955338435 28.042362321078052 22.30307558280585 96-97 20.59104894025275 29.618887982179487 27.68396704969013 22.10609602787764 98-99 20.350234886747096 29.844401314483584 27.064356763194457 22.741007035574867 100-101 20.128683946767254 29.9248769202984 27.341685244084523 22.604753888849828 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 870.0 1 564.5 2 294.0 3 361.5 4 346.5 5 341.5 6 562.5 7 1038.0 8 1492.0 9 1667.5 10 1542.0 11 1357.0 12 1345.5 13 1444.5 14 1534.5 15 1550.5 16 1521.0 17 1496.0 18 1503.0 19 1472.5 20 1472.5 21 1629.0 22 1822.5 23 2067.5 24 2310.0 25 2630.0 26 3085.5 27 3480.5 28 3891.0 29 4452.0 30 5088.5 31 5629.0 32 6244.5 33 6796.0 34 7487.5 35 8024.0 36 8712.5 37 9437.0 38 9678.0 39 9905.0 40 10132.0 41 10569.0 42 10819.0 43 11349.5 44 12112.0 45 12605.0 46 12265.5 47 12125.0 48 13156.5 49 12913.0 50 12267.5 51 11655.0 52 10648.0 53 10230.5 54 10076.5 55 9677.5 56 8622.5 57 7770.5 58 7409.0 59 6616.0 60 5477.5 61 4535.5 62 3697.5 63 2739.5 64 1865.5 65 1432.0 66 1112.0 67 903.5 68 655.0 69 368.0 70 256.0 71 190.5 72 177.0 73 164.0 74 124.5 75 98.5 76 72.0 77 47.0 78 30.0 79 21.5 80 16.5 81 7.5 82 2.0 83 1.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046040007939833705 2 0.0 3 0.0 4 0.0 5 0.009373414790145784 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004135330054476081 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.135330054476082E-4 24-25 5.513773405968109E-4 26-27 0.0022055093623872435 28-29 0.0022055093623872435 30-31 4.135330054476082E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010614013806488609 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.513773405968109E-4 74-75 4.135330054476082E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3784433514920272E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009097726119847379 92-93 0.003170419708431662 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.015576409871859907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 362728.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.5709588799741 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63867072941542 49.449325677671666 2 10.498222264075297 12.717747781402666 3 3.242884095800029 5.8927379765767665 4 1.4133545986408373 3.4243297310838585 5 0.7992046505906628 2.4204296013805555 6 0.49274291457444364 1.7907546490252328 7 0.3518114712408194 1.4916690710621603 8 0.23205586727684907 1.12446771157462 9 0.18644387319461847 1.0163775759024904 >10 1.0475930700661547 11.563770728697206 >50 0.06727085081773808 2.783796582610621 >100 0.02699986529416917 2.8356306920329044 >500 9.152496709887854E-4 0.4423916504872448 >1k 0.0018304993419775708 3.046570570492048 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 1.0815266535806445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3338 0.9202487814560774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2336 0.644008733817075 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1394 0.3843100063959771 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.2544606426854282 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 673 0.18553847511082686 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 411 0.11330804349264463 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3784433514920272E-4 0.0 12-13 0.0 0.0 0.0 2.7568867029840544E-4 0.0 14-15 0.0 0.0 0.0 2.7568867029840544E-4 0.0 16-17 0.0 0.0 0.0 2.7568867029840544E-4 0.0 18-19 0.0 0.0 0.0 2.7568867029840544E-4 0.0 20-21 0.0 0.0 0.0 4.135330054476082E-4 0.0 22-23 0.0 0.0 0.0 0.0011027546811936218 0.0 24-25 0.0 0.0 0.0 0.0022055093623872435 0.0 26-27 0.0 0.0 0.0 0.003859641384177676 0.0 28-29 1.3784433514920272E-4 0.0 0.0 0.005238084735669703 0.0 30-31 2.7568867029840544E-4 0.0 0.0 0.008822037449548974 0.0 32-33 2.7568867029840544E-4 0.0 0.0 0.013784433514920272 0.0 34-35 2.7568867029840544E-4 0.0 0.0 0.01847114090999316 0.0 36-37 2.7568867029840544E-4 0.0 0.0 0.022882159634767647 0.0 38-39 2.7568867029840544E-4 0.0 0.0 0.026741801018945327 0.0 40-41 2.7568867029840544E-4 0.0 0.0 0.03280695176551024 0.0 42-43 2.7568867029840544E-4 0.0 0.0 0.05141593701065261 0.0 44-45 2.7568867029840544E-4 0.0 0.0 0.0766414503429567 0.0 46-47 2.7568867029840544E-4 0.0 0.0 0.10531307205399087 0.0 48-49 2.7568867029840544E-4 0.0 0.0 0.16692948986568448 0.0 50-51 2.7568867029840544E-4 0.0 0.0 0.22124015791447035 0.0 52-53 2.7568867029840544E-4 0.0 0.0 0.2752751372929578 0.0 54-55 2.7568867029840544E-4 0.0 0.0 0.37410952559493615 0.0 56-57 2.7568867029840544E-4 0.0 0.0 0.4779063099622858 0.0 58-59 2.7568867029840544E-4 0.0 0.0 0.5963145938554509 0.0 60-61 2.7568867029840544E-4 0.0 0.0 0.712655212721378 0.0 62-63 2.7568867029840544E-4 0.0 0.0 0.8780684149004212 0.0 64-65 2.7568867029840544E-4 0.0 0.0 1.01315586334664 0.0 66-67 2.7568867029840544E-4 0.0 0.0 1.190010145343067 0.0 68-69 2.7568867029840544E-4 0.0 0.0 1.4242076707615623 0.0 70-71 2.7568867029840544E-4 0.0 0.0 1.582590811847996 0.0 72-73 2.7568867029840544E-4 0.0 0.0 1.870134094969233 0.0 74-75 2.7568867029840544E-4 0.0 0.0 2.229769965373503 0.0 76-77 2.7568867029840544E-4 0.0 0.0 2.4988421075847467 0.0 78-79 2.7568867029840544E-4 0.0 0.0 2.7476511325290573 0.0 80-81 2.7568867029840544E-4 0.0 0.0 3.111008799982356 0.0 82-83 2.7568867029840544E-4 0.0 0.0 3.536258573917646 0.0 84-85 2.7568867029840544E-4 0.0 0.0 3.9484131360137624 0.0 86-87 2.7568867029840544E-4 0.0 0.0 4.530805452019144 0.0 88-89 2.7568867029840544E-4 0.0 0.0 5.223473236143887 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2025 0.0 46.221813 1 GTATCAA 3400 0.0 38.14956 1 AGGTAAG 150 2.5465852E-11 34.832726 2 TAGGTAG 70 2.0507813E-4 33.927982 6 TAAGGTG 175 3.6379788E-12 32.57086 5 GTAAGGT 175 3.6379788E-12 32.57086 4 TGTGGCG 60 0.0039477553 31.666115 8 TATCAAC 4315 0.0 29.831463 2 CTAGACT 80 4.4881026E-4 29.686983 4 TAGGCCC 80 4.4881026E-4 29.686983 4 GGTAAGG 195 1.6370905E-11 29.230259 3 ATCAACG 4415 0.0 29.155777 3 TCAACGC 4490 0.0 28.668764 4 CAACGCA 4550 0.0 28.290716 5 AACGCAG 4565 0.0 28.197758 6 AGGACCG 70 0.008384843 27.142385 5 ACGGCGA 35 0.008390277 27.142385 84-85 TACCTGG 265 0.0 26.886322 2 GGTGTGC 370 0.0 25.675228 8 ACGCAGA 5270 0.0 24.425571 7 >>END_MODULE