##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 255398 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94884063305116 31.0 31.0 34.0 30.0 34.0 2 32.13878338906334 33.0 31.0 34.0 30.0 34.0 3 32.138842120924984 33.0 31.0 34.0 30.0 34.0 4 35.81896099421295 37.0 35.0 37.0 35.0 37.0 5 35.773024847492934 37.0 35.0 37.0 35.0 37.0 6 35.93927908597561 37.0 35.0 37.0 35.0 37.0 7 35.87712511452713 37.0 35.0 37.0 35.0 37.0 8 35.92896185561359 37.0 35.0 37.0 35.0 37.0 9 37.465242484279436 39.0 37.0 39.0 35.0 39.0 10-11 37.39878150964377 39.0 37.0 39.0 34.0 39.0 12-13 37.242574334959556 39.0 37.0 39.0 33.5 39.0 14-15 37.96611171583176 40.0 37.0 41.0 33.0 41.0 16-17 38.01010188020266 39.0 37.0 41.0 33.5 41.0 18-19 38.30595971777383 40.0 37.5 41.0 34.0 41.0 20-21 38.39912019671259 40.0 37.5 41.0 34.0 41.0 22-23 38.33721094135428 40.0 37.5 41.0 34.0 41.0 24-25 38.21802833225006 40.0 37.0 41.0 34.0 41.0 26-27 37.80523144268945 40.0 36.5 41.0 33.0 41.0 28-29 37.46833961111677 39.0 35.5 41.0 33.0 41.0 30-31 37.135637319007984 38.5 35.0 40.0 33.0 41.0 32-33 36.6951933844431 38.0 35.0 40.0 33.0 41.0 34-35 36.39061582314662 38.0 35.0 40.0 32.5 41.0 36-37 36.00959678619253 38.0 35.0 40.0 31.0 41.0 38-39 35.60719739387153 37.5 35.0 40.0 30.5 41.0 40-41 35.163170815746405 37.0 35.0 40.0 30.0 41.0 42-43 34.77378053077941 37.0 35.0 40.0 28.0 41.0 44-45 34.15346831220292 37.0 34.0 40.0 23.5 41.0 46-47 33.22381342062193 36.0 33.0 40.0 16.0 41.0 48-49 32.48642706677421 36.0 33.0 40.0 2.0 41.0 50-51 32.10028661148482 35.5 33.0 40.0 2.0 41.0 52-53 31.637812355617505 35.0 32.5 40.0 2.0 41.0 54-55 31.42145788142429 35.0 32.0 39.5 2.0 41.0 56-57 31.24915621892106 35.0 32.0 39.0 2.0 41.0 58-59 31.04213032208553 35.0 31.0 39.0 2.0 41.0 60-61 30.619893264630107 35.0 31.0 39.0 2.0 40.5 62-63 30.061639088794745 35.0 30.0 38.5 2.0 40.0 64-65 29.49949686371859 35.0 28.5 38.0 2.0 40.0 66-67 29.032891800249022 35.0 28.0 37.0 2.0 39.5 68-69 28.63605823068309 35.0 28.0 36.5 2.0 39.0 70-71 28.21486268490748 34.0 26.5 36.0 2.0 39.0 72-73 27.664237777899594 34.0 26.0 35.0 2.0 38.0 74-75 27.14128145091191 33.5 25.5 35.0 2.0 37.0 76-77 25.80400981996727 31.5 23.5 34.5 2.0 36.0 78-79 26.221436737954093 33.0 23.5 35.0 2.0 36.0 80-81 26.07500254504734 33.0 22.5 35.0 2.0 36.0 82-83 25.73144464717813 33.0 20.0 35.0 2.0 35.5 84-85 25.366823545994876 33.0 18.0 35.0 2.0 35.0 86-87 24.91107017282829 33.0 9.0 35.0 2.0 35.0 88-89 24.2572573003704 32.5 2.0 35.0 2.0 35.0 90-91 23.508079546433407 31.0 2.0 34.5 2.0 35.0 92-93 23.053765103877083 31.0 2.0 34.0 2.0 35.0 94-95 22.591384818988402 31.0 2.0 34.0 2.0 35.0 96-97 21.883949756850093 31.0 2.0 34.0 2.0 35.0 98-99 21.016801227887456 29.5 2.0 34.0 2.0 35.0 100-101 18.895147965136765 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01841064991333141 1101 2 0.03842949591426503 1101 3 -0.04612571224184592 1101 4 -0.07542181514489243 1101 5 -0.015146222025322231 1101 6 -0.12309016771165204 1101 7 -0.030483518041030777 1101 8 0.006187946349925255 1101 9 -0.21635108179737728 1101 10-11 -0.0685843483868851 1101 12-13 -0.28760822677529063 1101 14-15 -0.20404138128054683 1101 16-17 -0.025429160115969296 1101 18-19 -0.004396555117558876 1101 20-21 0.15876090403613574 1101 22-23 0.025033104880243684 1101 24-25 0.1032488892199197 1101 26-27 0.22550834430970923 1101 28-29 0.0030752005894214562 1101 30-31 -0.44524768727274733 1101 32-33 -0.5923381122523921 1101 34-35 -0.5788323336124463 1101 36-37 -0.7770582660969012 1101 38-39 -0.7123056888588408 1101 40-41 -0.10591777936977564 1101 42-43 -0.2659342401517648 1101 44-45 -0.6889021236321824 1101 46-47 -1.2340421836817512 1101 48-49 -1.1315433232284704 1101 50-51 -0.8090016595712513 1101 52-53 -0.8708341642100024 1101 54-55 -0.9989303119644362 1101 56-57 -0.7109066293260682 1101 58-59 -0.5750196202232765 1101 60-61 -0.47729862867689477 1101 62-63 -0.3807632998224797 1101 64-65 -0.48556294957307955 1101 66-67 -0.5072209678880633 1101 68-69 -0.43769822798266134 1101 70-71 -0.5172720879707882 1101 72-73 -0.7846871119190588 1101 74-75 -0.5003644349784224 1101 76-77 -0.5046180926457602 1101 78-79 -0.32471284619525065 1101 80-81 -0.4913431042328362 1101 82-83 -0.6413554285697565 1101 84-85 -1.0079838090812991 1101 86-87 -0.7944108542341723 1101 88-89 -0.625262864188084 1101 90-91 -0.5887812545368263 1101 92-93 -0.8112576134665375 1101 94-95 -0.6215388203854495 1101 96-97 -0.8747666175143145 1101 98-99 -1.235995155304689 1101 100-101 -0.9815111085708139 1102 1 0.1440859871595741 1102 2 0.07367297183570543 1102 3 0.1415928780191109 1102 4 0.20262499680022472 1102 5 0.24016720277962378 1102 6 0.2898100206122649 1102 7 -0.04776896329257596 1102 8 -0.06409722986745692 1102 9 0.0818060341563438 1102 10-11 -0.08136347133550004 1102 12-13 0.2671065970073343 1102 14-15 0.27241812907726626 1102 16-17 0.40223427814069623 1102 18-19 0.34568415513264483 1102 20-21 0.12445904770037686 1102 22-23 0.20572317751947367 1102 24-25 0.11508639258284603 1102 26-27 0.28022316809233416 1102 28-29 0.08919976340889946 1102 30-31 0.31292422556018096 1102 32-33 0.03942137120201039 1102 34-35 0.20826075220946194 1102 36-37 -0.047437604272843714 1102 38-39 0.052222156177478496 1102 40-41 0.3211403320103656 1102 42-43 -0.13652880566159098 1102 44-45 -0.5845504959754706 1102 46-47 -0.8700389552717454 1102 48-49 -0.6289202400969103 1102 50-51 -0.09851605289919974 1102 52-53 -0.12937599902302566 1102 54-55 -0.4314363652332034 1102 56-57 -0.6264971593233781 1102 58-59 -0.6161818478261836 1102 60-61 -0.6985456020425183 1102 62-63 -0.4557565739682943 1102 64-65 -0.1256288629440796 1102 66-67 -0.11251421513046722 1102 68-69 -0.5210651905869099 1102 70-71 -0.3815779798190526 1102 72-73 -0.5723182660756372 1102 74-75 -0.4583951048735031 1102 76-77 -0.25939344911603257 1102 78-79 -0.007521562902272905 1102 80-81 0.004621383257077838 1102 82-83 -0.08045281893833689 1102 84-85 -1.2562014577226783 1102 86-87 -0.9205206201744467 1102 88-89 -0.6305090304512007 1102 90-91 -0.5673594089624387 1102 92-93 -0.7679431125249216 1102 94-95 -1.1210680105926052 1102 96-97 -0.6653907767825409 1102 98-99 -0.45781382627589906 1102 100-101 -0.5055896665476745 1103 1 0.11560018066547073 1103 2 0.05513249893839145 1103 3 0.0761578520031776 1103 4 0.07923041075003567 1103 5 -0.1381506527166536 1103 6 0.18791974859751548 1103 7 0.11817320887366378 1103 8 0.13233957445316946 1103 9 -0.03878936859673132 1103 10-11 0.18813201703965632 1103 12-13 0.03771352580928067 1103 14-15 0.2140963217928018 1103 16-17 -0.3445795926358386 1103 18-19 -0.072834912245348 1103 20-21 -0.0702900137499256 1103 22-23 0.13555423857459914 1103 24-25 -0.26425234152767274 1103 26-27 -0.2712653387903643 1103 28-29 -0.1813083706884413 1103 30-31 0.0681850435803355 1103 32-33 0.0235895197892404 1103 34-35 0.1530756767961705 1103 36-37 -0.23885234444277614 1103 38-39 -0.942517025802367 1103 40-41 -0.7522888321864301 1103 42-43 -0.8564293523256126 1103 44-45 -0.43346046106800884 1103 46-47 -0.2795183071784848 1103 48-49 -0.3840575647363096 1103 50-51 -0.2865141428682456 1103 52-53 -0.07409212890512862 1103 54-55 -0.1492045928562007 1103 56-57 -0.30599067180444095 1103 58-59 -0.5687603896163438 1103 60-61 -0.6366013222559097 1103 62-63 -0.2462953344240688 1103 64-65 0.08731488993743497 1103 66-67 0.2437724212391572 1103 68-69 0.35158020228669784 1103 70-71 0.38845264653937406 1103 72-73 0.34993030140114456 1103 74-75 0.1886608129293066 1103 76-77 -0.22376149232224662 1103 78-79 0.16119509388492403 1103 80-81 0.9285000633598237 1103 82-83 0.7803866067351173 1103 84-85 0.3589108389248885 1103 86-87 0.17760722017331076 1103 88-89 0.02240047359938302 1103 90-91 0.18131509541746027 1103 92-93 -0.31318285434142723 1103 94-95 -0.36282688565023946 1103 96-97 -0.27548924217146364 1103 98-99 -0.263871588246527 1103 100-101 0.07286156179981873 1104 1 0.4481694485011012 1104 2 0.18625617716084975 1104 3 0.3882646650134802 1104 4 0.16671869879714762 1104 5 0.22378215413651503 1104 6 0.20871683064810753 1104 7 0.30009329989226785 1104 8 0.3608899693132912 1104 9 0.3647041396350943 1104 10-11 0.23820898130147583 1104 12-13 0.2102389574784027 1104 14-15 0.34455074556472454 1104 16-17 0.3080522504909169 1104 18-19 0.24122947003279904 1104 20-21 0.027780860296381604 1104 22-23 0.009006587657722775 1104 24-25 0.18283472847634386 1104 26-27 0.3886781092085627 1104 28-29 0.28594835531878005 1104 30-31 0.4122905850071916 1104 32-33 0.4344866553801978 1104 34-35 0.2543757035663461 1104 36-37 0.6822090931157874 1104 38-39 0.6717498059470657 1104 40-41 0.7361748942223514 1104 42-43 1.4677658691976063 1104 44-45 1.1955634313158683 1104 46-47 1.1058635024855548 1104 48-49 1.1093670102871158 1104 50-51 0.668445032610272 1104 52-53 0.045035163291363034 1104 54-55 0.044946123246059955 1104 56-57 0.3181392974098536 1104 58-59 0.20812690793418298 1104 60-61 0.26285992791140345 1104 62-63 0.2808345678647832 1104 64-65 0.11182633418853882 1104 66-67 0.24670877514091316 1104 68-69 0.6938285626132945 1104 70-71 0.31245043799968997 1104 72-73 0.31113027463096543 1104 74-75 0.0033944496579891847 1104 76-77 0.3657345590764933 1104 78-79 0.16903373663690147 1104 80-81 0.6547749930881075 1104 82-83 0.6091913182864452 1104 84-85 0.01706403126861744 1104 86-87 -0.14959128069671834 1104 88-89 -0.28047062799344147 1104 90-91 -0.2644920254498935 1104 92-93 0.2862683570091278 1104 94-95 0.4544248209486135 1104 96-97 0.7595964223982072 1104 98-99 0.911489809156766 1104 100-101 0.4114290226484343 1105 1 0.25165518563786904 1105 2 0.21128283671829706 1105 3 0.28182638188898324 1105 4 0.2779890267097045 1105 5 0.09901542526421281 1105 6 0.2865960628528512 1105 7 0.2752665870157003 1105 8 0.06603526390140502 1105 9 0.24047060191182368 1105 10-11 0.33654942385012276 1105 12-13 0.0036440494538751977 1105 14-15 0.205154584540999 1105 16-17 -0.09112463567277018 1105 18-19 0.09976719330659023 1105 20-21 -0.03645201093794981 1105 22-23 0.1356228233282053 1105 24-25 -0.3094839865329817 1105 26-27 0.46200855442317845 1105 28-29 0.20793582532678556 1105 30-31 0.38154878282110616 1105 32-33 0.45239854554282033 1105 34-35 0.10739823089239309 1105 36-37 0.4293431906897638 1105 38-39 0.08985497790120434 1105 40-41 0.1385842517270106 1105 42-43 -0.029451539493408063 1105 44-45 0.2312372516304535 1105 46-47 0.48557891170975864 1105 48-49 0.39479089725058714 1105 50-51 -0.15940087514766077 1105 52-53 -0.2083639302836815 1105 54-55 -0.7223159402036856 1105 56-57 -0.28486656769278795 1105 58-59 -0.13241561405995128 1105 60-61 -0.7602339137308256 1105 62-63 -0.6399032353876812 1105 64-65 -0.2290494322737402 1105 66-67 -0.34198424005503725 1105 68-69 -0.1998497898260574 1105 70-71 0.12171796665871071 1105 72-73 0.0299052679660754 1105 74-75 0.23709879516585275 1105 76-77 0.2822568198069675 1105 78-79 0.11423168385097782 1105 80-81 -0.059034657671691804 1105 82-83 -0.019174450841784818 1105 84-85 0.08542913390791185 1105 86-87 -0.6220652876282031 1105 88-89 0.04830836427528595 1105 90-91 -0.009393385087324901 1105 92-93 0.24387966066149147 1105 94-95 -0.22104505375146744 1105 96-97 0.1207334525235062 1105 98-99 0.5864764793780388 1105 100-101 0.5090863140373969 1106 1 0.18811950581238435 1106 2 0.20392033304753454 1106 3 0.17789132294699783 1106 4 0.22450075217851406 1106 5 0.19005037093690902 1106 6 0.28586191006517225 1106 7 0.155075287775027 1106 8 0.18821926356579155 1106 9 0.2070981137631449 1106 10-11 0.19651502354806638 1106 12-13 0.0822403493238184 1106 14-15 0.42075428248956115 1106 16-17 0.4077636472487427 1106 18-19 0.5192096567038291 1106 20-21 0.7936335703012958 1106 22-23 0.5836238301663172 1106 24-25 0.30999413571394285 1106 26-27 0.20639752954588886 1106 28-29 0.13761447616371925 1106 30-31 0.3108393814702666 1106 32-33 0.30044989428637336 1106 34-35 -0.2232012572982356 1106 36-37 0.010152436819737432 1106 38-39 0.3508987334073552 1106 40-41 0.33392133273552815 1106 42-43 0.9473282566218728 1106 44-45 0.7507971794737287 1106 46-47 0.5465974962631819 1106 48-49 0.419605261474274 1106 50-51 0.46474436124953655 1106 52-53 0.939746150682879 1106 54-55 0.38119960285961696 1106 56-57 0.6766008988508219 1106 58-59 0.9784519447771522 1106 60-61 1.0356820991902644 1106 62-63 0.6681053227362419 1106 64-65 0.4103976457981666 1106 66-67 0.5464979514959118 1106 68-69 1.0121210957719633 1106 70-71 0.8640408260789414 1106 72-73 0.28712094107930497 1106 74-75 0.3091296715345244 1106 76-77 0.45602029675456635 1106 78-79 0.5828518961260123 1106 80-81 0.5085493503830136 1106 82-83 0.6546309776936852 1106 84-85 0.5189778187256344 1106 86-87 0.524880636775034 1106 88-89 0.7997242303658147 1106 90-91 1.2938222041227263 1106 92-93 1.4198036443842739 1106 94-95 0.5067520683696038 1106 96-97 1.118017087209079 1106 98-99 1.1599546855281453 1106 100-101 0.926053633750449 1107 1 0.2494220222828467 1107 2 0.24351424167697644 1107 3 0.09475059500661587 1107 4 0.14051760065027707 1107 5 -0.21630020687927498 1107 6 -0.18773135054613022 1107 7 0.0751962511619979 1107 8 -0.13032485566954932 1107 9 0.3336128561759324 1107 10-11 0.11578276304477697 1107 12-13 0.26736833290735973 1107 14-15 0.30338739621674904 1107 16-17 0.4021582902987433 1107 18-19 0.3755980355784416 1107 20-21 0.10574070930118751 1107 22-23 0.29683260001187506 1107 24-25 0.46202999271793743 1107 26-27 0.21293171250300702 1107 28-29 0.443446639464284 1107 30-31 0.4745503653778158 1107 32-33 0.32516098619694844 1107 34-35 0.49487844893354094 1107 36-37 0.21340332784468075 1107 38-39 0.4574653172717049 1107 40-41 0.3432527940164505 1107 42-43 0.4339034807281479 1107 44-45 0.542534948131248 1107 46-47 0.025072493563108367 1107 48-49 -0.11975104050218022 1107 50-51 0.10468387955604896 1107 52-53 -0.5082935944299827 1107 54-55 -0.46768256584110546 1107 56-57 -0.3280000299751862 1107 58-59 -0.22013321560734056 1107 60-61 -0.028028884717286928 1107 62-63 -0.33770523995951507 1107 64-65 0.1271489148337004 1107 66-67 -0.2199779098282022 1107 68-69 0.06935189823443366 1107 70-71 -0.05495690585755497 1107 72-73 -0.1663405558426092 1107 74-75 -0.37445386880460774 1107 76-77 -0.3864788932227512 1107 78-79 -0.7252267300755229 1107 80-81 -0.25703008584120113 1107 82-83 -0.17934677368335272 1107 84-85 -0.1598319879489516 1107 86-87 -0.32399073162328307 1107 88-89 -0.46533679134278927 1107 90-91 -0.45737122929340757 1107 92-93 -0.8593733725722004 1107 94-95 -0.26013420444603597 1107 96-97 -0.3449162749250583 1107 98-99 0.11397779817529852 1107 100-101 0.14776224159146523 1108 1 0.1756939926806247 1108 2 0.0609055460248058 1108 3 0.13055078002049214 1108 4 -0.021184527009303622 1108 5 0.03864891430481521 1108 6 0.0806312766509123 1108 7 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-0.4364687451089466 2316 54-55 -0.5827649854184784 2316 56-57 -0.7588038931659469 2316 58-59 -0.9084782885867888 2316 60-61 -0.7451082544072172 2316 62-63 -0.4959943983358137 2316 64-65 -0.23394323533694816 2316 66-67 -0.9359607723654193 2316 68-69 -0.5210990494091661 2316 70-71 -0.5009303282669642 2316 72-73 -0.2258206226496675 2316 74-75 -0.12006420937537143 2316 76-77 -0.14787603213786582 2316 78-79 -0.5606054244332306 2316 80-81 -0.599271026949328 2316 82-83 -0.5376214087861051 2316 84-85 -0.7963580209167809 2316 86-87 -1.0052557087491252 2316 88-89 -0.7266613138901725 2316 90-91 -0.44112694783236606 2316 92-93 -0.13545354104217466 2316 94-95 -0.5686884125645442 2316 96-97 -0.8349896174564648 2316 98-99 -0.41308632207740814 2316 100-101 -0.5689395229862697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7.0 5 16.0 6 42.0 7 102.0 8 170.0 9 302.0 10 588.0 11 948.0 12 1548.0 13 2229.0 14 2584.0 15 3810.0 16 5211.0 17 7595.0 18 6393.0 19 3307.0 20 2543.0 21 2685.0 22 2841.0 23 3206.0 24 3489.0 25 3838.0 26 4176.0 27 4866.0 28 5717.0 29 6762.0 30 7912.0 31 9488.0 32 11368.0 33 14282.0 34 19891.0 35 31749.0 36 31739.0 37 32409.0 38 20151.0 39 1434.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.23360093988643 18.817701194439003 14.556882710005874 13.39181515566869 2 14.707632792739176 26.38313534170197 38.430606347739605 20.478625517819246 3 23.83495563786717 34.33229704226345 30.007282750843782 11.8254645690256 4 10.841510113626574 18.551828910171576 36.187440778706176 34.419220197495676 5 9.986722492254788 45.9194184529941 28.89600852267164 15.197850532079476 6 22.9187385962302 37.29551523504491 18.70883875363159 21.076907415093306 7 22.858049005865354 43.018347833577394 17.774219062013014 16.349384098544235 8 27.358867336470922 30.185436064495413 19.27148998817532 23.184206610858347 9 30.87377348295601 18.92771282468931 20.44221176360034 29.756301928754336 10-11 24.209077596535604 29.73045990963124 22.41423190471343 23.646230589119728 12-13 32.540291634689176 28.69222516328097 24.250160540041037 14.517322661988816 14-15 27.700490998363335 27.466933961894767 30.824634492047704 14.007940547694187 16-17 16.445312805895114 32.13885778275476 32.33717570223729 19.078653709112835 18-19 15.910852864940212 29.993774422665798 39.18981354591657 14.905559166477419 20-21 18.86545705134731 27.719872512705656 37.223666590967824 16.19100384497921 22-23 17.229612662614162 28.24616529135955 36.11977407766325 18.40444796836304 24-25 17.626836597852368 28.99813036541078 37.89798255660294 15.477050480133908 26-27 19.495291607118386 26.705691184244014 39.20906830595744 14.589948902680161 28-29 16.519085105258316 27.4433183043554 41.39834100444213 14.639255585944152 30-31 15.588414944517966 32.3919921064378 38.79924666598799 13.22034628305625 32-33 14.610725221027574 32.01728282915293 37.52554835981488 15.846443590004618 34-35 15.732503778416431 29.299368045168716 37.9145490567663 17.05357911964855 36-37 17.83118896780711 30.10223259383394 37.924141927501395 14.142436510857564 38-39 19.892481538618156 27.723983743020696 38.59955833640044 13.783976381960706 40-41 15.717686582066351 30.423614999393102 39.788642779672436 14.070055638868114 42-43 14.850354973744082 33.116328792227776 37.213113470206096 14.820202763822046 44-45 15.686888699206728 33.491452556402166 34.30528038590748 16.51637835848362 46-47 15.329603207542739 32.51532901588893 32.381028825597696 19.774038950970642 48-49 17.032044103712636 31.929185036687834 32.70170479017064 18.337066069428893 50-51 19.083548030916454 33.41803772934792 31.858511029843616 15.639903209892012 52-53 16.17416737797477 33.95425962615212 33.015137158474225 16.856435837398884 54-55 14.051429634197673 33.51540246086982 33.505026478332795 18.928141426599712 56-57 16.970962967603505 35.367348217292225 28.94795573966907 18.713733075435204 58-59 16.366612111292962 33.20875652902529 30.500630388648304 19.924000971033447 60-61 17.0279328733976 31.778244152264307 31.778244152264307 19.415578822073783 62-63 21.273659151598682 31.26101222405814 30.786654554851644 16.67867406949154 64-65 19.69357630051919 32.110862262038076 32.535297848847684 15.660263588595056 66-67 17.101347700451843 32.65276157213447 33.102647632322885 17.1432430950908 68-69 17.306909216203728 34.780421146602556 31.58814869341185 16.324520943781863 70-71 17.14696277966155 34.869302030556234 29.13413574107863 18.849599448703593 72-73 16.949819497411884 32.96952207926452 28.94227832637687 21.138380096946726 74-75 17.45510928041723 33.722660318405 30.213823130956392 18.60840727022138 76-77 18.20903061104629 31.676442258749088 32.52159374779755 17.592933382407068 78-79 16.092725863162592 30.977533105192677 34.76084385938809 18.168897172256635 80-81 15.101527811494217 35.14612487176877 32.416463715455876 17.33588360128114 82-83 14.9030809319736 34.126553729832594 30.51780879309771 20.452556545096094 84-85 16.022443402062663 31.27373746074754 30.42310433127902 22.280714805910776 86-87 17.34175678744548 31.19053399008606 31.38806881808002 20.079640404388442 88-89 18.652456166453927 28.542705894329636 32.90491703145679 19.899920907759654 90-91 15.839195822474759 30.207956016157173 33.82569757583708 20.12715058553099 92-93 14.159607354084155 32.776615093061544 32.90935039967423 20.15442715318007 94-95 14.117964901839484 33.09188012435493 30.90685909834846 21.88329587545713 96-97 14.397920109006336 31.04429165459401 29.554851643317487 25.00293659308217 98-99 16.542251301418002 29.8027169731533 30.02609688149118 23.628934843937515 100-101 18.06109521823497 30.101955900695305 31.155976667821918 20.680972213247806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3637.0 1 2395.0 2 1499.5 3 1828.5 4 1477.5 5 1400.5 6 2706.0 7 5177.0 8 6706.5 9 6457.0 10 4991.5 11 3552.5 12 3186.0 13 2943.5 14 2632.5 15 2286.5 16 1851.5 17 1602.5 18 1440.5 19 1333.5 20 1272.0 21 1260.0 22 1351.0 23 1412.5 24 1438.0 25 1516.0 26 1671.0 27 1878.5 28 1978.5 29 2147.0 30 2487.5 31 2691.0 32 2997.5 33 3420.0 34 3829.5 35 4089.5 36 4393.5 37 4832.0 38 4892.5 39 4892.0 40 4836.0 41 4783.0 42 4831.5 43 4837.5 44 5387.5 45 5445.5 46 5713.0 47 8548.5 48 25446.0 49 25444.0 50 11127.5 51 8170.0 52 3552.0 53 2114.5 54 1741.5 55 1609.0 56 1869.5 57 2184.0 58 2223.0 59 2437.5 60 2678.5 61 2760.5 62 2285.5 63 1140.5 64 449.5 65 335.0 66 303.0 67 233.5 68 168.0 69 144.5 70 135.5 71 126.0 72 103.5 73 123.5 74 123.5 75 73.5 76 45.0 77 32.0 78 31.5 79 25.5 80 16.5 81 9.0 82 5.5 83 5.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018794195725886656 2 0.0 3 0.0 4 0.0 5 0.030932113798855118 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003915457442893054 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.9577287214465268E-4 24-25 1.9577287214465268E-4 26-27 0.001174637232867916 28-29 9.788643607232634E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.9154574428930535E-4 42-43 0.010571735095811243 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.9577287214465268E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001761955849301874 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012333690945113118 92-93 9.788643607232634E-4 94-95 0.0 96-97 0.0 98-99 0.0013704101050125686 100-101 0.016640694132295478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 255398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.088739927485726 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9718866681309 24.378421130940726 2 11.851770505405472 7.606167628564045 3 4.550113478292701 4.380222241364459 4 2.2134368060131293 2.8410559205631993 5 1.3141518412768138 2.108473832997909 6 0.8248529662981672 1.5881095388374222 7 0.5917953974180639 1.3292978018621915 8 0.4270688434975718 1.0963280840100549 9 0.2989481904483003 0.8633583661579183 >10 1.6143202284208216 9.689974079671728 >50 0.1549649803548332 3.523128607115169 >100 0.14032262000634502 9.450739629910961 >500 0.01830295043561022 4.17074526816968 >1k 0.021963540522732264 13.891259915895974 >5k 0.0061009834785367405 13.082717953938557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8093 3.1687797085333482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6981 2.7333808408836404 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6748 2.6421506824642322 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6060 2.3727672103931905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5531 2.1656395116641476 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4951 1.9385429799763507 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3536 1.3845057518069837 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2317 0.9072114895183204 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2184 0.8551359055278427 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2172 0.8504373565963711 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2117 0.8289023406604593 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.8061926874916796 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1945 0.7615564726426989 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1814 0.7102639801407998 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1697 0.6644531280589511 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1631 0.638611108935857 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1573 0.6159014557670772 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1369 0.5360261239320591 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1312 0.5137080165075686 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1307 0.5117502877861221 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1296 0.5074432845989397 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1118 0.43774814211544333 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1080 0.4228694038324497 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 985 0.38567255812496576 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 968 0.37901628047204755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 956 0.37431773154057585 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 949 0.37157691133055076 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 887 0.34730107518461384 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 862 0.3375124315773812 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 613 0.24001754124934419 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 599 0.23453590082929388 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 595 0.23296971785213666 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 575 0.22513880296635055 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 542 0.21221779340480348 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 542 0.21221779340480348 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 541 0.21182624766051417 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 537 0.21026006468335695 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 501 0.19616441788894196 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 483 0.18911659449173446 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 477 0.18676732002599863 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 457 0.17893640514021253 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 449 0.17580403918589807 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 441 0.17267167323158367 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 410 0.16053375515861518 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 394 0.1542690232499863 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 391 0.15309438601711836 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 391 0.15309438601711836 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 389 0.15231129452853978 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 373 0.1460465626199109 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 360 0.14095646794414993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 349 0.13664946475696757 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.13508328177981033 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 343 0.13430019029123172 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 329 0.12881854987118146 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.12803545838260283 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 324 0.12686082114973493 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 324 0.12686082114973493 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 321 0.125686183916867 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 321 0.125686183916867 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 311 0.12177072647397397 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 309 0.12098763498539535 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 305 0.11942145200823813 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 300 0.1174637232867916 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 299 0.11707217754250228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 295 0.11550599456534506 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 266 0.10415116798095521 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 261 0.10219343925950868 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 257 0.10062725628235147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.915457442893053E-4 0.0 22-23 0.0 0.0 0.0 0.001761955849301874 0.0 24-25 0.0 0.0 0.0 0.0029365930821697896 0.0 26-27 0.0 0.0 0.0 0.004307003187182358 0.0 28-29 0.0 0.0 0.0 0.00665627765291819 0.0 30-31 0.0 0.0 0.0 0.014682965410848951 0.0 32-33 0.0 0.0 0.0 0.02290542604092436 0.0 34-35 0.0 0.0 0.0 0.031127886670999777 0.0 36-37 0.0 0.0 0.0 0.03993766591750914 0.0 38-39 0.0 0.0 0.0 0.048943218036163166 0.0 40-41 0.0 0.0 0.0 0.06793318663419448 0.0 42-43 0.0 0.0 0.0 0.08770624672080439 0.0 44-45 0.0 0.0 0.0 0.12803545838260283 0.0 46-47 0.0 0.0 0.0 0.20340801415829413 0.0 48-49 0.0 0.0 0.0 0.2672299704774509 0.0 50-51 0.0 0.0 0.0 0.334771611367356 0.0 52-53 0.0 0.0 0.0 0.4216947665995818 0.0 54-55 0.0 0.0 0.0 0.4960884580145498 0.0 56-57 0.0 0.0 0.0 0.5802707930367506 0.0 58-59 0.0 0.0 0.0 0.7051738854650389 0.0 60-61 0.0 0.0 0.0 0.8890046124088677 0.0 62-63 0.0 0.0 0.0 1.0575650553254137 0.0 64-65 0.0 0.0 0.0 1.166610545109985 0.0 66-67 0.0 0.0 0.0 1.329102028990047 0.0 68-69 0.0 0.0 0.0 1.50216524796592 0.0 70-71 0.0 0.0 0.0 1.672291873859623 0.0 72-73 0.0 0.0 0.0 1.9544005826200674 0.0 74-75 0.0 0.0 0.0 2.1850210260064684 0.0 76-77 0.0 0.0 0.0 2.3731587561374794 0.0 78-79 0.0 0.0 0.0 2.5977102404873964 0.0 80-81 0.0 0.0 0.0 2.819912450371577 0.0 82-83 0.0 0.0 0.0 3.0924282883969334 0.0 84-85 0.0 0.0 0.0 3.555431131019037 0.0 86-87 0.0 0.0 0.0 4.13746387990509 0.0 88-89 0.0 0.0 0.0 4.572275428938363 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGTAC 20 0.002073071 71.24266 6 GATATAG 40 5.3674175E-4 47.52302 1 CATGAGA 70 7.870585E-8 47.513714 4 GGAGAGT 95 1.913395E-8 40.011547 8 ACTCGTA 25 0.0016157929 37.996082 56-57 ATGGGGA 405 0.0 36.368523 5 GGAATGA 55 0.00257224 34.555428 8 GGGACTT 70 2.0454047E-4 33.938366 7 GTCACTA 75 3.0630722E-4 31.682014 1 ATGAGAG 75 3.0665955E-4 31.67581 5 TCCATGC 60 0.0039396756 31.675808 8 TATATGG 60 0.0039396756 31.675808 2 TGGGGAT 165 7.6397555E-11 31.6634 6 ATGGGTA 95 3.4734745E-5 30.008661 5 TGGGGAC 95 3.482779E-5 29.996908 6 TGGGAGT 190 1.2732926E-11 29.996908 6 TCTAGAT 80 4.4763857E-4 29.696072 2 GACATAT 65 0.005821868 29.244934 1 ATAGGCT 65 0.005827446 29.239208 4 GGGATCT 100 4.928523E-5 28.508228 7 >>END_MODULE