##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937798_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 253946 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811526860041113 33.0 31.0 34.0 30.0 34.0 2 31.76733242500374 33.0 31.0 34.0 30.0 34.0 3 31.71278933316532 33.0 31.0 34.0 30.0 34.0 4 35.36053334173407 37.0 35.0 37.0 33.0 37.0 5 35.35095256471848 37.0 35.0 37.0 33.0 37.0 6 35.58276956518315 37.0 35.0 37.0 33.0 37.0 7 35.556807352744286 37.0 35.0 37.0 33.0 37.0 8 35.584691233569345 37.0 35.0 37.0 33.0 37.0 9 37.10067888448725 39.0 37.0 39.0 34.0 39.0 10-11 37.00928937648162 39.0 37.0 39.0 33.0 39.0 12-13 36.83060965717121 39.0 37.0 39.0 32.5 39.0 14-15 37.50943704567113 39.5 37.0 41.0 32.0 41.0 16-17 37.58432698290187 39.0 37.0 41.0 32.5 41.0 18-19 37.913924613894295 40.0 37.5 41.0 33.0 41.0 20-21 37.97693210367558 40.0 37.5 41.0 33.0 41.0 22-23 37.878452505650806 40.0 37.5 41.0 33.5 41.0 24-25 37.66003992974885 40.0 36.5 41.0 33.0 41.0 26-27 37.48445929449568 39.5 36.5 41.0 33.0 41.0 28-29 37.315783670544135 39.0 36.0 41.0 33.0 41.0 30-31 36.801286493979035 38.5 35.0 40.0 33.0 41.0 32-33 36.20405519283627 38.0 35.0 40.0 32.0 41.0 34-35 35.82806580926653 37.5 35.0 40.0 31.0 41.0 36-37 35.45844195222607 37.0 35.0 40.0 30.0 41.0 38-39 35.144924511510325 37.0 35.0 40.0 30.0 41.0 40-41 34.66387539083112 36.5 35.0 40.0 28.0 41.0 42-43 34.186903908705 36.0 35.0 40.0 24.5 41.0 44-45 33.54937860805053 36.0 33.5 40.0 20.5 41.0 46-47 32.51825781859135 35.0 33.0 40.0 5.5 41.0 48-49 31.7214014002977 35.0 33.0 40.0 2.0 41.0 50-51 30.624012585352794 35.0 30.0 39.0 2.0 40.0 52-53 30.62162231340521 35.0 31.0 39.0 2.0 40.0 54-55 30.86537098438251 35.0 31.0 39.0 2.0 41.0 56-57 30.654940420404337 35.0 31.0 39.0 2.0 41.0 58-59 30.46995030439542 35.0 31.0 39.0 2.0 41.0 60-61 30.068223165554883 35.0 30.5 39.0 2.0 40.5 62-63 29.54291069754987 35.0 29.0 38.0 2.0 40.0 64-65 29.01621801485355 35.0 28.0 38.0 2.0 40.0 66-67 28.5702787206729 35.0 26.5 37.0 2.0 40.0 68-69 28.169872728847864 35.0 26.0 37.0 2.0 39.0 70-71 27.659652052011054 34.5 26.0 36.0 2.0 39.0 72-73 27.06503154213888 34.0 24.5 35.0 2.0 38.5 74-75 26.548565443046947 34.0 22.5 35.0 2.0 37.0 76-77 25.982283635103528 33.0 19.0 35.0 2.0 37.0 78-79 25.465819504934117 33.0 15.0 35.0 2.0 36.0 80-81 24.96816646058611 33.0 7.0 35.0 2.0 36.0 82-83 24.474571365565907 32.5 2.0 35.0 2.0 35.5 84-85 23.978802580076078 32.0 2.0 35.0 2.0 35.0 86-87 23.28135312231734 31.0 2.0 35.0 2.0 35.0 88-89 22.599412867302497 31.0 2.0 35.0 2.0 35.0 90-91 21.922686319138712 30.5 2.0 34.0 2.0 35.0 92-93 21.363181542532665 30.0 2.0 34.0 2.0 35.0 94-95 20.75487308325392 29.0 2.0 34.0 2.0 35.0 96-97 19.856601403447975 28.0 2.0 34.0 2.0 35.0 98-99 19.001622392162112 27.0 2.0 34.0 2.0 35.0 100-101 17.185712710576265 21.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3814187089696368 1101 2 0.5650443732898118 1101 3 0.8669187400015481 1101 4 0.2110468946763362 1101 5 0.5215671330656946 1101 6 0.13258433086483734 1101 7 0.08619654547330668 1101 8 0.0913122731494127 1101 9 0.4093286653192578 1101 10-11 0.4077749268838815 1101 12-13 0.18911065676321925 1101 14-15 0.6687390437708203 1101 16-17 0.844286779938777 1101 18-19 0.668723115001363 1101 20-21 0.6292151400817048 1101 22-23 0.6773772195717029 1101 24-25 0.5794362305524388 1101 26-27 0.8481715785579951 1101 28-29 0.9090051543624256 1101 30-31 0.9581783726683071 1101 32-33 0.7883820617652617 1101 34-35 0.9998765443970967 1101 36-37 0.8090284853050989 1101 38-39 1.0089434294735327 1101 40-41 0.9196926343296425 1101 42-43 1.2058327328386085 1101 44-45 1.249431301340259 1101 46-47 1.132649775521081 1101 48-49 1.2232478814772456 1101 50-51 1.3623090292084683 1101 52-53 0.9811500206910004 1101 54-55 1.0453492178397958 1101 56-57 0.8290831880830609 1101 58-59 1.347449078742219 1101 60-61 1.362483031127649 1101 62-63 1.0524639103390108 1101 64-65 1.3780442658828065 1101 66-67 1.1722632863500166 1101 68-69 1.2567003751011505 1101 70-71 1.5315679928089772 1101 72-73 1.4809386028242315 1101 74-75 1.861569623983165 1101 76-77 2.085670773439798 1101 78-79 1.8000400860333556 1101 80-81 1.9946985502064045 1101 82-83 1.8515154533363436 1101 84-85 1.4802045506943173 1101 86-87 1.8862576069887922 1101 88-89 1.569952497124408 1101 90-91 1.7960330283637624 1101 92-93 2.0345682024375797 1101 94-95 1.9319983017937332 1101 96-97 1.6061929055917439 1101 98-99 1.3969338851058701 1101 100-101 2.04979935817941 1102 1 0.26685693173287817 1102 2 0.2982914822368592 1102 3 0.3889363906406196 1102 4 0.08870970660573363 1102 5 0.25230359076502396 1102 6 0.15187830530184243 1102 7 0.048323751803188486 1102 8 0.024696326709971572 1102 9 -0.1254096183082538 1102 10-11 0.17756342353634835 1102 12-13 0.32335441817527055 1102 14-15 0.34063496586455244 1102 16-17 0.11133638432100668 1102 18-19 -0.08829910045816547 1102 20-21 0.08581130563252515 1102 22-23 0.31898555797705797 1102 24-25 0.2104389694447164 1102 26-27 0.14342416528957358 1102 28-29 0.5777437423113057 1102 30-31 0.3155429496616122 1102 32-33 0.49866812384682646 1102 34-35 0.3693835437884516 1102 36-37 0.10883067641891131 1102 38-39 0.15059741608339294 1102 40-41 0.13294582970395652 1102 42-43 0.428322081590899 1102 44-45 1.0249638637261356 1102 46-47 0.750346062800439 1102 48-49 0.9387074067359187 1102 50-51 0.6692627723612432 1102 52-53 0.7573140499058475 1102 54-55 0.7649703377424153 1102 56-57 1.0820427133417319 1102 58-59 1.190000813373988 1102 60-61 0.6664924650899131 1102 62-63 0.6090372517875835 1102 64-65 0.5172865056880411 1102 66-67 0.7990283502575011 1102 68-69 0.5900996447298787 1102 70-71 0.4002076763147606 1102 72-73 0.9547289254048756 1102 74-75 0.6182038297044095 1102 76-77 0.7225240905426595 1102 78-79 0.7845196965020094 1102 80-81 1.0000774303037865 1102 82-83 0.8993167314860706 1102 84-85 1.192225244547025 1102 86-87 0.8450220622535483 1102 88-89 0.6993042926204538 1102 90-91 1.2191538439450191 1102 92-93 1.2547599248964971 1102 94-95 1.145738411349427 1102 96-97 0.9231512135649638 1102 98-99 1.346873020833197 1102 100-101 0.8293413545275534 1103 1 0.05130794332642097 1103 2 0.1098850262241271 1103 3 0.07347075451119878 1103 4 0.0037661588297268622 1103 5 0.056092330630143294 1103 6 -0.06323118928025906 1103 7 0.13178211355207736 1103 8 0.008238040735882635 1103 9 -0.3838926068662616 1103 10-11 -0.28476134724488844 1103 12-13 -0.052071815059555604 1103 14-15 0.11181781045043948 1103 16-17 -0.16344576313674253 1103 18-19 0.01974818301535919 1103 20-21 0.18484896570678444 1103 22-23 -0.04787054568286209 1103 24-25 -0.26494937639146343 1103 26-27 -0.48451400247581944 1103 28-29 -0.2124888863164145 1103 30-31 0.07551870172647313 1103 32-33 -0.23979516326388506 1103 34-35 0.07261912763325995 1103 36-37 -0.31878923719781227 1103 38-39 -0.3432799802945681 1103 40-41 -0.39804606239273 1103 42-43 -0.07908111610554869 1103 44-45 0.14371812605848078 1103 46-47 0.013511933831736656 1103 48-49 -0.15400305776359247 1103 50-51 0.11138598218165541 1103 52-53 -0.23413855295843788 1103 54-55 0.12595389461139916 1103 56-57 0.09615955807917231 1103 58-59 0.3320406709173689 1103 60-61 0.2783966857461202 1103 62-63 0.528011261032109 1103 64-65 0.16174357926536942 1103 66-67 0.1833884320942829 1103 68-69 0.31537053963023354 1103 70-71 0.6232735246136158 1103 72-73 0.31215864428037676 1103 74-75 0.23943592790854495 1103 76-77 0.2460218551861395 1103 78-79 0.54003231300576 1103 80-81 0.3543549176115057 1103 82-83 0.0833055168160719 1103 84-85 0.7193223120623671 1103 86-87 0.8914720129999338 1103 88-89 1.0109584564455645 1103 90-91 1.220820889485669 1103 92-93 1.3663307364445778 1103 94-95 0.9111244839416841 1103 96-97 1.0120502057601612 1103 98-99 0.9654984660101285 1103 100-101 1.267630359604464 1104 1 0.34777249003785826 1104 2 0.6156128555743159 1104 3 0.5360752709375625 1104 4 0.2620780950993975 1104 5 0.49948886759768385 1104 6 0.4646837491620346 1104 7 0.3550357839714593 1104 8 0.5943529923456055 1104 9 0.6062482845683306 1104 10-11 0.6445213573439545 1104 12-13 0.667462058138149 1104 14-15 0.6695322748654817 1104 16-17 0.7160147016733092 1104 18-19 0.56781750950163 1104 20-21 0.7873655895793235 1104 22-23 0.7320771138075628 1104 24-25 0.5425840359464118 1104 26-27 0.8127405896632212 1104 28-29 0.6016611564777108 1104 30-31 0.6001006360210326 1104 32-33 0.5468431934416245 1104 34-35 0.500074852170755 1104 36-37 0.7139729591284762 1104 38-39 1.006134069346615 1104 40-41 1.0875932160324453 1104 42-43 1.0204810670533178 1104 44-45 1.7711129512609318 1104 46-47 1.5247042440562453 1104 48-49 0.7221902400176923 1104 50-51 1.1140276965802336 1104 52-53 1.6120001000141109 1104 54-55 1.355667832329484 1104 56-57 1.73273866137761 1104 58-59 1.750509628715779 1104 60-61 1.7651469655538783 1104 62-63 1.2752296427557255 1104 64-65 1.0663044456818547 1104 66-67 1.2620967078201382 1104 68-69 1.4024565887447267 1104 70-71 1.3867372154425048 1104 72-73 1.5571879417983183 1104 74-75 1.315033399763177 1104 76-77 1.5192376692621146 1104 78-79 1.330645585768945 1104 80-81 1.0798717389954042 1104 82-83 1.3049461778207458 1104 84-85 1.8709369674050507 1104 86-87 1.5575955234351149 1104 88-89 1.56281341727248 1104 90-91 1.609194054275978 1104 92-93 1.475406383294267 1104 94-95 0.6852373287635913 1104 96-97 0.515070483592801 1104 98-99 -0.09810842055251356 1104 100-101 -0.4643796476376174 1105 1 0.1385705740964127 1105 2 0.32913238603373784 1105 3 0.26133331586773423 1105 4 0.042588113314330656 1105 5 0.276777540605849 1105 6 0.0823387447769548 1105 7 0.1365914744524659 1105 8 0.30294774374545597 1105 9 0.17295205573476125 1105 10-11 0.32838258651484153 1105 12-13 0.44948998770706083 1105 14-15 0.9624301362970371 1105 16-17 1.0937806734738942 1105 18-19 0.9978333632424423 1105 20-21 1.0828607659943543 1105 22-23 1.0536466341475688 1105 24-25 1.0228933525489836 1105 26-27 0.6308610780933748 1105 28-29 0.5829149512560932 1105 30-31 0.6059614604155499 1105 32-33 0.5047633175730439 1105 34-35 0.6806810047714436 1105 36-37 0.6326213433961598 1105 38-39 0.7024337387792556 1105 40-41 0.38506167720764495 1105 42-43 0.09561767907516838 1105 44-45 0.36923748745469 1105 46-47 0.3315247257401097 1105 48-49 0.4564882903448897 1105 50-51 0.41950735656809 1105 52-53 0.11709353589078475 1105 54-55 0.09327998911286173 1105 56-57 0.2673133088848658 1105 58-59 0.12186664146623727 1105 60-61 0.20893364841159823 1105 62-63 0.1077871547303566 1105 64-65 0.30929675493099396 1105 66-67 -0.09049723402699072 1105 68-69 0.01783471732868236 1105 70-71 -0.028816316231246475 1105 72-73 -0.2263410334428606 1105 74-75 -0.28634621300077256 1105 76-77 0.04395432580937353 1105 78-79 -0.29580486285819063 1105 80-81 0.2240311534189381 1105 82-83 0.6865828235739677 1105 84-85 0.39912795438474546 1105 86-87 0.480941000059282 1105 88-89 0.47508657385347064 1105 90-91 1.2307618880478195 1105 92-93 1.41504410845112 1105 94-95 1.6335946113649555 1105 96-97 1.2407923347700276 1105 98-99 0.7699260529009031 1105 100-101 0.9193483426477513 1106 1 0.3072948539318041 1106 2 0.6048525506427929 1106 3 0.48767076442740276 1106 4 0.5857979898575394 1106 5 0.4413865941037898 1106 6 0.4527091151473286 1106 7 0.3711593756870357 1106 8 0.07661029518578744 1106 9 0.5227462944178853 1106 10-11 -0.16132934764153362 1106 12-13 -0.1266416995357318 1106 14-15 -0.03962747687168644 1106 16-17 0.08349260763026933 1106 18-19 0.12540537970334498 1106 20-21 0.06228463430711173 1106 22-23 0.2511774983451005 1106 24-25 0.5784489081045407 1106 26-27 0.43744544023329013 1106 28-29 0.36480795537132593 1106 30-31 0.18415076082707316 1106 32-33 0.46361105419855164 1106 34-35 0.524302403948397 1106 36-37 0.8239793680875209 1106 38-39 0.5748617223183459 1106 40-41 0.25543204757801163 1106 42-43 -0.13071976948449304 1106 44-45 -0.09989831501444968 1106 46-47 -0.057364163148776015 1106 48-49 0.3782989899333664 1106 50-51 0.21374603969566053 1106 52-53 0.24466555235169096 1106 54-55 0.12002896030627497 1106 56-57 -0.2985303125143126 1106 58-59 -0.5489152515378812 1106 60-61 -0.3589675861563002 1106 62-63 -0.43542272181285213 1106 64-65 -0.7462588006245596 1106 66-67 -0.36415978546731864 1106 68-69 -0.4801076695025941 1106 70-71 -0.15433071952342559 1106 72-73 -0.14609411986261378 1106 74-75 -0.1258523858402789 1106 76-77 -0.18238312275029855 1106 78-79 -0.013911858742961414 1106 80-81 -0.5743227560460831 1106 82-83 -0.7208245838334371 1106 84-85 -0.9815304818292461 1106 86-87 -0.9573306048789902 1106 88-89 -0.4055718623605209 1106 90-91 -0.08816815310444426 1106 92-93 -0.41623161171348855 1106 94-95 -0.3972695861659119 1106 96-97 -0.7345163072052827 1106 98-99 -0.355588350391276 1106 100-101 -0.3625446614674779 1107 1 0.39388372590880394 1107 2 0.41301535576145554 1107 3 0.43992286432029815 1107 4 0.2922075958105239 1107 5 0.3991632444222972 1107 6 0.5115061161056715 1107 7 0.47809888749716833 1107 8 0.3738649318620375 1107 9 0.6049944482033283 1107 10-11 0.7972928971298714 1107 12-13 0.9017984605634837 1107 14-15 0.8537994355811023 1107 16-17 0.7773310427168028 1107 18-19 0.7086682468322678 1107 20-21 0.8952797298592827 1107 22-23 0.5244736255225035 1107 24-25 0.5671461289174431 1107 26-27 0.5300098463441145 1107 28-29 0.7095004408552867 1107 30-31 0.669070993083686 1107 32-33 1.117001887954686 1107 34-35 1.0876656149523996 1107 36-37 0.9504521117596525 1107 38-39 0.7665016118273371 1107 40-41 0.7936247303673554 1107 42-43 1.0385400535043985 1107 44-45 0.7259374141698274 1107 46-47 0.6545418067480568 1107 48-49 1.0434717730665817 1107 50-51 1.2135205478415543 1107 52-53 0.7213271454516423 1107 54-55 0.9971922727816143 1107 56-57 1.1416016002203406 1107 58-59 1.0906642061542087 1107 60-61 1.1007821178856396 1107 62-63 0.37699624205207627 1107 64-65 -0.015073277201594237 1107 66-67 -0.027895058030598108 1107 68-69 0.12812842046865924 1107 70-71 -0.058412122082760476 1107 72-73 0.11226317198022073 1107 74-75 0.5735669726353976 1107 76-77 0.4765604479132115 1107 78-79 0.6243146478244874 1107 80-81 0.8208235317942645 1107 82-83 0.8777435428929827 1107 84-85 1.4566001907002075 1107 86-87 0.8239141101556768 1107 88-89 0.6521258658090474 1107 90-91 0.5864752879012478 1107 92-93 0.5772291245174124 1107 94-95 0.777796652066236 1107 96-97 1.0756195516368194 1107 98-99 1.4170031147420445 1107 100-101 1.7120931422418657 1108 1 0.2826034959071144 1108 2 0.4073216864452611 1108 3 0.2819094475549768 1108 4 -0.056177318784435215 1108 5 0.27266231426840193 1108 6 0.24283257193744845 1108 7 0.3423527913248918 1108 8 0.200067085309243 1108 9 0.6173965001792041 1108 10-11 0.4394936976259558 1108 12-13 0.5132759959375193 1108 14-15 0.7772449511118538 1108 16-17 0.6884308792352059 1108 18-19 0.643923898221864 1108 20-21 0.4820377207268649 1108 22-23 0.43636268353029095 1108 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-2.0549285623109164 2316 54-55 -1.961711836807428 2316 56-57 -1.719128993386967 2316 58-59 -1.5805686705581188 2316 60-61 -1.2759302108814836 2316 62-63 -1.2234603789524883 2316 64-65 -1.8826531858773947 2316 66-67 -1.705977249811422 2316 68-69 -2.004291670855544 2316 70-71 -2.197681528475453 2316 72-73 -2.31696057231202 2316 74-75 -1.9652458127527304 2316 76-77 -2.391019460762589 2316 78-79 -2.008105443499712 2316 80-81 -1.8641079750550489 2316 82-83 -1.8756811146412637 2316 84-85 -1.954358713409384 2316 86-87 -2.089327898976741 2316 88-89 -2.069335321405564 2316 90-91 -2.397542976938933 2316 92-93 -2.8177480444323564 2316 94-95 -2.5190070009018015 2316 96-97 -1.9883073890024505 2316 98-99 -1.1925859263538463 2316 100-101 -1.3381351682604183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1006.0 3 182.0 4 139.0 5 148.0 6 184.0 7 217.0 8 277.0 9 359.0 10 528.0 11 982.0 12 1639.0 13 2668.0 14 3089.0 15 4234.0 16 5875.0 17 8346.0 18 6530.0 19 3159.0 20 2819.0 21 3072.0 22 3329.0 23 3728.0 24 3872.0 25 4146.0 26 4658.0 27 5226.0 28 5977.0 29 7070.0 30 8390.0 31 9802.0 32 11820.0 33 14770.0 34 20057.0 35 32089.0 36 28487.0 37 26471.0 38 16706.0 39 1895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.98240389286716 16.926230641319844 14.157065498595866 13.934299967217129 2 14.831880349979468 24.26324267980669 40.37201111848132 20.532865851732524 3 22.60389976983541 34.322948949257196 30.784415133223053 12.288736147684338 4 10.791795803146654 18.006198551039343 37.85498549065282 33.34702015516118 5 9.898605429821375 42.82025364436091 30.316897515675095 16.964243410142615 6 22.346948438313788 37.89357806852796 18.958428686415935 20.801044806742315 7 21.769955496638577 43.520578859325184 18.11381497964208 16.595650664394153 8 25.865871315682536 29.332512733111614 19.62457539856316 25.177040552642687 9 31.048864407582187 18.06886156662826 20.422512981581146 30.4597610442084 10-11 23.58737918666659 28.561958818411565 22.85248689524283 24.998175099679017 12-13 32.96593166605181 27.99742746496795 24.011576407644217 15.02506446133602 14-15 28.24108964216524 25.721765618033388 31.893221696568432 14.143923043232942 16-17 16.292015149124193 31.10324285939719 32.751301877860186 19.853440113618433 18-19 15.39315846472808 28.429792497233365 41.22181826789733 14.955230770141222 20-21 18.528833939546956 25.68544219404622 39.179481312577416 16.606242553829404 22-23 16.96943036763647 26.22376173953182 38.082340943028875 18.72446694980284 24-25 17.315108702727976 26.502910641051187 40.332943406722386 15.849037249498455 26-27 19.185170280592114 24.253937442792665 41.39424139128239 15.166650885332828 28-29 16.18012906382366 25.24714873905315 43.82724939384195 14.745472803281242 30-31 15.47534968139044 29.79837834638679 41.39695002860581 13.329321943616959 32-33 14.131318312749084 29.772323718581067 39.75200252535217 16.34435544331768 34-35 15.073476465633057 27.065853615231493 40.38414115688197 17.476528762253476 36-37 17.552507416524648 27.573691851290793 40.341751877800924 14.532048854383639 38-39 19.490880260023037 25.532313542340525 40.754824153110654 14.221982044525788 40-41 15.59920166923705 27.74869935983655 42.385683689045436 14.266415281880967 42-43 14.604968588814266 30.84509403631271 39.60768070061245 14.942256674260578 44-45 15.279158579115974 31.41204689990374 36.73700863198094 16.57178588899935 46-47 14.911688936756102 30.14983489756548 34.5866915996986 20.351784565979823 48-49 16.473983877777297 29.780144334534146 34.735182825058494 19.01068896263006 50-51 18.843707584987847 30.823087970707995 33.98705059815031 16.346153846153847 52-53 15.640600643264468 31.292843189486767 35.334162078967616 17.732394088281143 54-55 14.13204930692952 30.728261534243224 35.87515065677477 19.264538502052485 56-57 16.848565647322395 32.8021646438869 31.21891381262104 19.130355896169668 58-59 15.984427755514183 30.396839796787734 32.928118393234676 20.690614054463413 60-61 17.155956970587365 28.673128309291158 34.11964227610211 20.051272444019368 62-63 21.44567240944087 28.26837214438975 32.8292629054651 17.45669254070428 64-65 20.012700340976547 29.21275634869141 35.19256596190292 15.58197734842912 66-67 17.699783463557587 29.825627028796585 35.49028346947388 16.984306038171944 68-69 17.175605638691458 32.11206386520151 34.30821901598996 16.404111480117063 70-71 16.970906566533085 31.72667318797135 31.94163958221577 19.360780663279794 72-73 16.708934649622744 30.322906354396327 31.559787174460936 21.408371821519992 74-75 17.23214990138067 30.448520710059174 33.63629191321499 18.68303747534517 76-77 18.138481777104573 28.4978906448769 35.119825965875584 18.243801612142942 78-79 16.130355631906873 28.27757868141448 37.50120805580922 18.090857630869426 80-81 15.068957832477729 31.90618658157298 35.42280923539934 17.602046350549948 82-83 14.580839799268453 31.218290626941013 33.179662621143855 21.02120695264668 84-85 15.94594967633297 28.51858497467254 33.131357745234716 22.404107603759783 86-87 17.655236604070492 28.268058598379454 34.14958796165746 19.92711683589259 88-89 19.060108341336342 25.79170618870797 35.29375804819947 19.854427421756217 90-91 16.445545394123112 27.141411770738628 36.820587057749236 19.592455777389027 92-93 14.878767859431852 29.92141356971711 35.7168916453849 19.482926925466145 94-95 14.777368253949472 30.46679800352204 34.19550260011477 20.56033114241372 96-97 15.166073055578563 28.566695459762638 32.3585095431364 23.90872194152241 98-99 17.11389738643693 27.199714403491853 33.23175722718926 22.45463098288196 100-101 19.484509653118774 26.74120964720267 34.58064643357195 19.193634266106606 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2155.0 1 1519.0 2 1293.0 3 1642.0 4 1250.5 5 1158.0 6 2305.0 7 4863.0 8 7430.0 9 7689.0 10 5311.0 11 3289.5 12 2945.5 13 2828.5 14 2601.0 15 2214.0 16 1815.5 17 1559.5 18 1487.0 19 1434.0 20 1322.5 21 1335.5 22 1424.0 23 1489.0 24 1542.5 25 1669.0 26 1840.0 27 1979.5 28 2142.0 29 2327.0 30 2591.0 31 2835.0 32 3175.5 33 3541.0 34 3923.0 35 4201.0 36 4366.0 37 4698.5 38 4832.0 39 4778.5 40 4683.5 41 4697.0 42 4665.5 43 4627.5 44 5176.0 45 5439.0 46 6013.0 47 9330.5 48 24316.5 49 24663.5 50 11793.5 51 8025.0 52 3786.5 53 2292.0 54 1923.5 55 1803.0 56 1895.0 57 2048.5 58 2044.0 59 2186.0 60 2425.0 61 2565.0 62 2171.5 63 1113.5 64 453.0 65 374.0 66 328.0 67 225.5 68 166.5 69 142.5 70 131.5 71 114.0 72 92.0 73 124.0 74 123.0 75 69.0 76 50.5 77 42.5 78 37.5 79 27.0 80 14.5 81 6.5 82 4.5 83 4.0 84 1.5 85 1.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30124514660597135 2 0.2654107566175486 3 0.2555661439833665 4 0.26029155804777393 5 0.26698589463901773 6 0.19767982169437595 7 0.1898041315870303 8 0.18626007103872477 9 0.20083009773731422 10-11 0.19984563647389603 12-13 0.19669536043095776 14-15 0.1988611752104778 16-17 0.1835035795011538 18-19 0.1888196703236121 20-21 0.18114087246895008 22-23 0.187244532302143 24-25 0.1888196703236121 26-27 0.1898041315870303 28-29 0.19905806746316146 30-31 0.19728603718900867 32-33 0.2031928047695179 34-35 0.19590779142022322 36-37 0.1803533034582155 38-39 0.1693273373079316 40-41 0.16420813873815693 42-43 0.17858127318406275 44-45 0.18507871752262292 46-47 0.18389736400652107 48-49 0.1996487442212124 50-51 0.19610468367290684 52-53 0.21697526245737284 54-55 0.18606317878604112 56-57 0.16420813873815693 58-59 0.1646019232435242 60-61 0.15692312538886222 62-63 0.16145164720058597 64-65 0.1612547549479023 66-67 0.16105786269521866 68-69 0.1622392162113205 70-71 0.1646019232435242 72-73 0.15948272467374952 74-75 0.17562788939380813 76-77 0.17031179857134982 78-79 0.1730682901089208 80-81 0.1659801690123097 82-83 0.14707851275468012 84-85 0.14471580572247641 86-87 0.15337906484055666 88-89 0.15574177187276034 90-91 0.1683428760445134 92-93 0.1588920479156986 94-95 0.15731690989422947 96-97 0.1626330007166878 98-99 0.17385585911965537 100-101 0.1579075866522804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 253946.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.42915422963937 #Duplication Level Percentage of deduplicated Percentage of total 1 77.45723978702351 25.89330015042568 2 11.125901144984216 7.438589306387971 3 4.2006313904726005 4.212706638419191 4 2.2381378692927485 2.992762240791349 5 1.1838571361259012 1.9787671394705961 6 0.7927719926494841 1.590101832673088 7 0.5324412194317485 1.2459341749820827 8 0.37105969938274513 0.9923369535255527 9 0.28624605380954626 0.8612067132382476 >10 1.4901286340291193 8.921975538106526 >50 0.1472459124534703 3.415686799555811 >100 0.1307543702586816 9.329148716656297 >500 0.017669509494416435 4.370220440566105 >1k 0.02002544409367196 12.867696281886701 >5k 0.004711869198511049 9.770974931678388 >10k+ 0.0011779672996277623 4.118592141636411 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10459 4.118592141636411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7761 3.056161546155482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5990 2.358769187150024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5813 2.289069329700015 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5249 2.0669748686728675 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3998 1.5743504524583964 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3389 1.3345356886897215 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2779 1.094327140415679 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2355 0.9273625101399511 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2330 0.917517897505769 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1901 0.7485843447032046 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1878 0.7395273010797571 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1638 0.6450190197916092 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1617 0.6367495451788963 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1610 0.6339930536413253 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1583 0.6233608719964087 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1487 0.5855575594811495 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.5851637749757822 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1237 0.4871114331393288 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1197 0.4713600529246375 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1154 0.4544273191938444 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1038 0.40874831657123956 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 986 0.38827152229214085 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 974 0.3835461082277335 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 0.35204334779835084 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 845 0.33274790703535395 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 832 0.3276287084655793 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 822 0.32369086341190645 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 800 0.31502760429382626 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 703 0.2768305072731998 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 681 0.2681672481551196 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 663 0.2610791270585085 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 663 0.2610791270585085 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 616 0.2425712553062462 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 553 0.21776283146810738 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 542 0.21343120190906728 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 524 0.20634308081245617 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.194923330156805 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 494 0.1945295456514377 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 492 0.19374197664070314 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 492 0.19374197664070314 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 491 0.19334819213533586 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 437 0.17208382884550258 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 424 0.16696463027572792 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 402 0.1583013711576477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 387 0.15239460357713847 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 384 0.15121325006103659 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 382 0.15042568105030205 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 379 0.14924432753420017 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 377 0.1484567585234656 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 374 0.14727540500736377 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 363 0.14294377544832365 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 332 0.13073645578193788 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 315 0.12404211919069409 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.1212856276531231 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 306 0.12049805864238854 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 296 0.11656021358871571 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 293 0.11537886007261386 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.1145912910618793 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.11183479952430833 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 284 0.11183479952430833 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 281 0.11065344600820647 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 263 0.10356532491159538 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 262 0.10317154040622808 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 255 0.10041504886865711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.937845053672828E-4 0.0 20-21 0.0 0.0 0.0 5.906767580509242E-4 0.0 22-23 0.0 0.0 0.0 9.84461263418207E-4 0.0 24-25 0.0 0.0 0.0 0.001968922526836414 0.0 26-27 0.0 0.0 0.0 0.006103659833192884 0.0 28-29 0.0 0.0 0.0 0.012994888677120332 0.0 30-31 0.0 0.0 0.0 0.021067471037149628 0.0 32-33 0.0 0.0 0.0 0.028943161144495286 0.0 34-35 0.0 0.0 0.0 0.041347373063564695 0.0 36-37 0.0 0.0 0.0 0.051782662455797684 0.0 38-39 0.0 0.0 0.0 0.06714025816512172 0.0 40-41 0.0 0.0 0.0 0.08879840596032228 0.0 42-43 0.0 0.0 0.0 0.1203011663897049 0.0 44-45 0.0 0.0 0.0 0.16184543170595322 0.0 46-47 0.0 0.0 0.0 0.2502500531609082 0.0 48-49 0.0 0.0 0.0 0.3225095098958046 0.0 50-51 0.0 0.0 0.0 0.387090168776039 0.0 52-53 0.0 0.0 0.0 0.4664377466075465 0.0 54-55 0.0 0.0 0.0 0.5536610145463996 0.0 56-57 0.0 0.0 0.0 0.632024131114489 0.0 58-59 0.0 0.0 0.0 0.778511967111118 0.0 60-61 0.0 0.0 0.0 0.989974246493349 0.0 62-63 0.0 0.0 0.0 1.178793916816961 0.0 64-65 0.0 0.0 0.0 1.304017389523757 0.0 66-67 0.0 0.0 0.0 1.4837800162239216 0.0 68-69 0.0 0.0 0.0 1.6798846998968284 0.0 70-71 0.0 0.0 0.0 1.853149882258433 0.0 72-73 0.0 0.0 0.0 2.1469131232624257 0.0 74-75 0.0 0.0 0.0 2.37963976593449 0.0 76-77 0.0 0.0 0.0 2.5599930693927053 0.0 78-79 0.0 0.0 0.0 2.797445126129177 0.0 80-81 0.0 0.0 0.0 3.0193426949036413 0.0 82-83 0.0 0.0 0.0 3.2973545556929427 0.0 84-85 0.0 0.0 0.0 3.782103281800068 0.0 86-87 0.0 0.0 0.0 4.376324100399298 0.0 88-89 0.0 0.0 0.0 4.839414678711222 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTATG 20 0.0020677068 71.289055 3 CGTCACT 45 2.030738E-7 63.38056 1 TACTATA 25 0.0050019934 57.042503 2 TAGCTAT 35 2.7762636E-4 54.31547 4 GTCACTA 55 8.123334E-7 51.85682 2 ACTATAC 50 0.0016091017 38.020832 3 GTGTACA 60 0.0039308234 31.690279 1 ATGGGGC 75 3.0618723E-4 31.684029 5 CACTACC 90 2.3966077E-5 31.684029 4 AGTACTC 90 2.3966077E-5 31.684029 5 GGAGAGT 90 2.4030818E-5 31.67153 8 GAGAGTA 90 2.4030818E-5 31.67153 9 CGGTCGA 45 2.4142944E-5 31.659044 68-69 ACTACCT 95 3.4672346E-5 30.016445 5 TCACTAC 95 3.4672346E-5 30.016445 3 GTCGGGA 255 0.0 29.826145 2 TCGGGAG 255 0.0 29.820261 3 ATGGGGA 320 0.0 29.703773 5 CGGGAGT 260 0.0 29.246796 4 GGATGAT 65 0.0058312193 29.235258 8 >>END_MODULE