##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 253946 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.987028738393203 31.0 31.0 34.0 30.0 34.0 2 32.09572113756468 33.0 31.0 34.0 30.0 34.0 3 32.1209902892741 33.0 31.0 34.0 30.0 34.0 4 35.79320406700637 37.0 35.0 37.0 35.0 37.0 5 35.75857071975932 37.0 35.0 37.0 35.0 37.0 6 35.90687784017074 37.0 35.0 37.0 35.0 37.0 7 35.83990297149788 37.0 35.0 37.0 35.0 37.0 8 35.8990533420491 37.0 35.0 37.0 35.0 37.0 9 37.41024075984658 39.0 37.0 39.0 34.0 39.0 10-11 37.36664290833484 39.0 37.0 39.0 34.0 39.0 12-13 37.22397871988533 39.0 37.0 39.0 33.5 39.0 14-15 37.94559670166099 40.0 37.0 41.0 33.0 41.0 16-17 38.00538303418837 39.0 37.0 41.0 33.5 41.0 18-19 38.301764942153056 40.0 37.5 41.0 34.0 41.0 20-21 38.35685342553141 40.0 37.5 41.0 34.0 41.0 22-23 38.22229135327983 40.0 37.5 41.0 34.0 41.0 24-25 38.15880935316957 40.0 37.0 41.0 33.5 41.0 26-27 37.752254416293226 39.5 36.5 41.0 33.0 41.0 28-29 37.433253526340245 39.0 35.5 41.0 33.0 41.0 30-31 37.10714679498791 38.5 35.0 40.0 33.0 41.0 32-33 36.690560197837335 38.0 35.0 40.0 33.0 41.0 34-35 36.390902002787996 38.0 35.0 40.0 32.0 41.0 36-37 35.987050396540994 38.0 35.0 40.0 31.0 41.0 38-39 35.600094114496784 37.5 35.0 40.0 30.5 41.0 40-41 35.175056901861026 37.0 35.0 40.0 30.0 41.0 42-43 34.77594842998118 37.0 35.0 40.0 28.0 41.0 44-45 34.130041426129964 37.0 33.5 40.0 23.5 41.0 46-47 33.231283422459896 36.0 33.0 40.0 16.5 41.0 48-49 32.498255534641224 36.0 33.0 40.0 2.0 41.0 50-51 32.102543454120166 35.5 33.0 40.0 2.0 41.0 52-53 31.633758751860633 35.0 32.0 40.0 2.0 41.0 54-55 31.41529498397297 35.0 32.0 39.5 2.0 41.0 56-57 31.268202688760603 35.0 32.0 39.5 2.0 41.0 58-59 31.050512707425987 35.0 31.0 39.0 2.0 41.0 60-61 30.609970229891395 35.0 31.0 39.0 2.0 40.5 62-63 30.083511848975768 35.0 30.0 38.5 2.0 40.0 64-65 29.518411394548448 35.0 29.0 38.0 2.0 40.0 66-67 29.038476684019436 35.0 28.0 37.0 2.0 39.5 68-69 28.655157001882287 35.0 28.0 37.0 2.0 39.0 70-71 28.218453923275028 34.0 26.5 36.0 2.0 39.0 72-73 27.654914824411488 34.0 26.0 35.0 2.0 38.0 74-75 27.100576500515857 33.5 25.5 35.0 2.0 37.0 76-77 25.78261717057957 31.5 23.5 34.5 2.0 36.0 78-79 26.209826104762428 33.0 23.5 35.0 2.0 36.0 80-81 26.037425279390106 33.0 22.0 35.0 2.0 36.0 82-83 25.711428807699274 33.0 20.0 35.0 2.0 35.5 84-85 25.324569002858876 33.0 17.5 35.0 2.0 35.0 86-87 24.75199845636474 33.0 8.5 35.0 2.0 35.0 88-89 24.259348444157418 32.5 2.0 35.0 2.0 35.0 90-91 23.55756735683964 31.5 2.0 35.0 2.0 35.0 92-93 23.067016216045932 31.0 2.0 34.0 2.0 35.0 94-95 22.60960007245635 31.0 2.0 34.0 2.0 35.0 96-97 21.955772093279673 31.0 2.0 34.0 2.0 35.0 98-99 21.08563828530475 30.0 2.0 34.0 2.0 35.0 100-101 19.089733644160567 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12363696406831082 1101 2 0.010877123082984497 1101 3 -0.027683773962415614 1101 4 -0.05298840599562027 1101 5 0.08235641536405325 1101 6 0.06119483497142397 1101 7 0.16397733320198427 1101 8 0.18429822907160798 1101 9 0.4095005469285695 1101 10-11 0.2830217419292893 1101 12-13 0.1665874350083172 1101 14-15 0.04194998880998213 1101 16-17 0.0131496466186789 1101 18-19 0.05811885128496641 1101 20-21 0.12679404463035837 1101 22-23 -0.10415517484049275 1101 24-25 0.3021678301267272 1101 26-27 0.16448019673853054 1101 28-29 0.18425909147597253 1101 30-31 0.21815975746609695 1101 32-33 0.12963822872668374 1101 34-35 0.4117050578096908 1101 36-37 0.2797761068902602 1101 38-39 0.1449049094744126 1101 40-41 0.4274443725656951 1101 42-43 0.3558039733213576 1101 44-45 0.3886396424838665 1101 46-47 0.17757436255372738 1101 48-49 -0.12028305582556698 1101 50-51 -0.027741159601170295 1101 52-53 0.0968263659270363 1101 54-55 0.04842710555153573 1101 56-57 0.24857468927282156 1101 58-59 -0.031020135667386484 1101 60-61 0.14842697872424537 1101 62-63 0.27124181941776726 1101 64-65 0.09872022037558281 1101 66-67 0.27782102360507466 1101 68-69 0.3499965932340139 1101 70-71 0.20760783465176047 1101 72-73 -0.17096852754409753 1101 74-75 -0.2656991974239524 1101 76-77 -0.2623357399206192 1101 78-79 0.10329034803114112 1101 80-81 -0.05052963995688131 1101 82-83 -0.1432358289907576 1101 84-85 -0.178253621472944 1101 86-87 -0.5637049803064436 1101 88-89 -0.12452479748545286 1101 90-91 -0.15724882518358996 1101 92-93 -0.1815500487351862 1101 94-95 -0.47088853431585775 1101 96-97 -0.2565674028972822 1101 98-99 -1.0595996842475621 1101 100-101 -0.5930848987447668 1102 1 0.19837829090945291 1102 2 0.2509866787737991 1102 3 0.2992182345585874 1102 4 0.08458006631114046 1102 5 0.12940449813946486 1102 6 0.08993798774075401 1102 7 0.14459206235596866 1102 8 0.21308703004543617 1102 9 0.48503310931445043 1102 10-11 0.21033458429082685 1102 12-13 0.05033667420490673 1102 14-15 -0.043549098225931004 1102 16-17 0.4015702187428474 1102 18-19 0.3136955402685331 1102 20-21 0.21637864596937106 1102 22-23 0.49718383915828923 1102 24-25 0.5890667954340927 1102 26-27 0.5284561553508169 1102 28-29 0.29673626737372416 1102 30-31 0.2472452717692022 1102 32-33 0.27244863651731777 1102 34-35 0.5451347595747578 1102 36-37 0.8118896187587907 1102 38-39 0.27609024118469705 1102 40-41 0.45884272923033365 1102 42-43 0.35468559231100727 1102 44-45 0.5198858384668199 1102 46-47 0.6619931087497122 1102 48-49 -0.09574714590469569 1102 50-51 -0.4020260043972712 1102 52-53 -0.39452329931946295 1102 54-55 -0.887984945574452 1102 56-57 -0.4768285365336311 1102 58-59 -0.19116164510134936 1102 60-61 -0.2786424065466022 1102 62-63 -0.36774935526269203 1102 64-65 0.03459210108161059 1102 66-67 -0.2838877408501901 1102 68-69 -0.2519891036619093 1102 70-71 0.0880247549195623 1102 72-73 0.06927797276022574 1102 74-75 -0.13399651939595358 1102 76-77 -0.1513725628055873 1102 78-79 0.041505503235036656 1102 80-81 0.30551113910580696 1102 82-83 0.6327532154401609 1102 84-85 0.13665812533168165 1102 86-87 -0.09002117020297362 1102 88-89 -0.14825425579342877 1102 90-91 0.07943711516942287 1102 92-93 0.1293903042775959 1102 94-95 0.07136648698663706 1102 96-97 0.2592953481678464 1102 98-99 -0.31064198491707273 1102 100-101 0.0437760263921767 1103 1 0.09500631242949709 1103 2 0.3157741304673607 1103 3 0.20894014105248715 1103 4 0.2733653963103819 1103 5 -0.02549434250613558 1103 6 -0.010948577387686953 1103 7 0.07960759618941893 1103 8 -0.028877052700643446 1103 9 -0.24038933518050953 1103 10-11 -0.014701408737899158 1103 12-13 0.040599612751542224 1103 14-15 0.09423979318878395 1103 16-17 0.05530212750998942 1103 18-19 0.004428282558443186 1103 20-21 0.19713184745455692 1103 22-23 0.19264492879987927 1103 24-25 0.07148704245992832 1103 26-27 -0.4716044644870223 1103 28-29 -0.055214933656813514 1103 30-31 0.10634961366508122 1103 32-33 0.16846288217920602 1103 34-35 -0.1236460239366437 1103 36-37 0.049799749849192665 1103 38-39 -0.39413026347545355 1103 40-41 -0.3922294841514926 1103 42-43 -0.5624092019604205 1103 44-45 -0.2982773804465708 1103 46-47 0.1216733391050937 1103 48-49 -0.5253144718120986 1103 50-51 0.17089208304684433 1103 52-53 -0.24919481557315848 1103 54-55 0.17135429819306935 1103 56-57 0.080199575916172 1103 58-59 0.2403152056980744 1103 60-61 0.24618899452940468 1103 62-63 0.16769051743564845 1103 64-65 0.2992886230184091 1103 66-67 0.7004874959460494 1103 68-69 0.8965294990508283 1103 70-71 0.674135234574031 1103 72-73 1.0381421085486622 1103 74-75 0.8378682983656596 1103 76-77 0.7058677190766574 1103 78-79 1.2449456790325684 1103 80-81 0.18886341613846724 1103 82-83 0.24033069517040317 1103 84-85 0.2633838963402475 1103 86-87 0.17490267612573618 1103 88-89 0.36324078095735146 1103 90-91 0.1848777456157329 1103 92-93 0.3330729594264916 1103 94-95 0.39627366285994015 1103 96-97 0.4718057672024756 1103 98-99 0.14155169546483748 1103 100-101 -0.07872908840663939 1104 1 0.21591618141362545 1104 2 0.30318278146479827 1104 3 0.272818605730869 1104 4 0.23369853213235814 1104 5 0.20292754175221006 1104 6 0.20404253460124977 1104 7 0.20509314494180586 1104 8 0.12004651040423653 1104 9 0.08282154111154227 1104 10-11 0.16635066842321322 1104 12-13 0.30586559471217356 1104 14-15 0.3571932463456733 1104 16-17 0.29395345412793716 1104 18-19 0.5129377301850155 1104 20-21 0.5917266199873126 1104 22-23 0.5142345660373522 1104 24-25 0.1085533404387391 1104 26-27 0.11956348600346445 1104 28-29 -0.33411742888362284 1104 30-31 -0.05904145882158218 1104 32-33 0.13919005315820954 1104 34-35 0.1871545554300056 1104 36-37 0.35992549874641355 1104 38-39 -0.044115450123683786 1104 40-41 -0.226673818867404 1104 42-43 0.1469528363567818 1104 44-45 0.26023535903595274 1104 46-47 0.004121163188308685 1104 48-49 -0.49359743974942205 1104 50-51 -0.39593259270534986 1104 52-53 0.13837184450979478 1104 54-55 -0.2074560250671844 1104 56-57 -0.721910503746745 1104 58-59 -0.523848004519845 1104 60-61 -0.23219174152959 1104 62-63 0.03409877341036349 1104 64-65 0.15705575712862085 1104 66-67 0.3271005053607574 1104 68-69 -0.13298198952643148 1104 70-71 0.2909632021821693 1104 72-73 0.4447110070936624 1104 74-75 0.24186954927091975 1104 76-77 -0.028035105335742827 1104 78-79 -0.1183846916304141 1104 80-81 -0.09534719680511117 1104 82-83 -0.02041721450106948 1104 84-85 -0.02608545648087457 1104 86-87 -0.17293951230009696 1104 88-89 -0.23442035539132533 1104 90-91 -0.20338843385625083 1104 92-93 -0.5117324918869564 1104 94-95 -0.184796519508879 1104 96-97 0.11859785358077346 1104 98-99 0.459470913319862 1104 100-101 0.583970891842224 1105 1 0.33205326567457405 1105 2 0.2759109741383412 1105 3 0.14269987133162942 1105 4 0.05684499371233187 1105 5 -0.13462221200883562 1105 6 -0.10004570664546009 1105 7 0.20118030678488452 1105 8 0.03780944638211281 1105 9 0.4099651692569921 1105 10-11 0.12320825460684404 1105 12-13 0.1186649605625405 1105 14-15 0.03947753427659251 1105 16-17 -0.06701429868862618 1105 18-19 0.12332361698256022 1105 20-21 0.00921970505781644 1105 22-23 0.10153976741359116 1105 24-25 0.19804264759653023 1105 26-27 -0.023069584225233086 1105 28-29 0.13753136016043044 1105 30-31 0.2313434160350596 1105 32-33 0.31138023058517916 1105 34-35 0.39117870708621183 1105 36-37 0.3572269896726823 1105 38-39 0.5403731824068458 1105 40-41 0.3142507578039826 1105 42-43 0.09726653803660668 1105 44-45 0.09527212622208481 1105 46-47 0.22118248680700958 1105 48-49 0.01866391085437158 1105 50-51 0.12329428444502355 1105 52-53 -0.32376470004933466 1105 54-55 -0.6225373176530304 1105 56-57 -0.40522359826202603 1105 58-59 -0.29156540315709734 1105 60-61 0.057012535607292136 1105 62-63 -0.5294932794804126 1105 64-65 -0.8301178919302714 1105 66-67 -0.9350174869484924 1105 68-69 -0.8987008048809493 1105 70-71 -0.8698679386125434 1105 72-73 -0.6826670540275686 1105 74-75 -0.4773927458110485 1105 76-77 -0.5315263014498903 1105 78-79 -0.7809108334370656 1105 80-81 -0.8758150508157705 1105 82-83 -0.6763235762362108 1105 84-85 -0.4471372003923655 1105 86-87 -0.3890816562655566 1105 88-89 -0.5186323907417894 1105 90-91 -0.5664981282500996 1105 92-93 -0.37169673664173075 1105 94-95 -0.29235740461985316 1105 96-97 -0.3258044266593956 1105 98-99 -0.351506958905734 1105 100-101 -0.21396137558002692 1106 1 0.09748536444000422 1106 2 0.04957352557867267 1106 3 0.266156661455085 1106 4 -0.21064471822182185 1106 5 0.04233252050145353 1106 6 -0.038317311583732305 1106 7 0.024225574274595374 1106 8 -0.003342816992372377 1106 9 0.1383602309853913 1106 10-11 -0.1278205519775213 1106 12-13 -0.1940922410835455 1106 14-15 0.010670949914448613 1106 16-17 0.4329857013113667 1106 18-19 0.1562454276821441 1106 20-21 0.05860242110719582 1106 22-23 -0.01985940954114085 1106 24-25 0.17952412907801119 1106 26-27 0.11684811124801797 1106 28-29 0.14164658649787754 1106 30-31 -0.05054958808017318 1106 32-33 0.24347899601728074 1106 34-35 0.2615490774565856 1106 36-37 0.5444697880265963 1106 38-39 0.6576571330241237 1106 40-41 0.34099972899739583 1106 42-43 0.5087891717814585 1106 44-45 0.9244902332179663 1106 46-47 0.840524050593018 1106 48-49 0.7717503306074605 1106 50-51 0.6479856424697203 1106 52-53 0.7029842711440786 1106 54-55 0.7745820239107566 1106 56-57 0.6194677597626672 1106 58-59 0.23312595486759236 1106 60-61 0.6475475150311638 1106 62-63 0.6248277081739104 1106 64-65 0.39004671712322647 1106 66-67 0.23164917971817545 1106 68-69 0.6486242979997137 1106 70-71 0.6815723906055595 1106 72-73 0.852312369840746 1106 74-75 0.4403027274399811 1106 76-77 0.22979057097809275 1106 78-79 0.26847188261231736 1106 80-81 0.47192157469863716 1106 82-83 0.574705230348151 1106 84-85 0.9849615650397361 1106 86-87 0.8722352161624265 1106 88-89 0.6974169919742543 1106 90-91 0.4129257400626578 1106 92-93 0.5892086131525076 1106 94-95 0.8907701673966066 1106 96-97 1.2873642976204422 1106 98-99 1.4633078559090826 1106 100-101 1.281923398082526 1107 1 0.22748141833255886 1107 2 0.31835981567394356 1107 3 0.5081094209116443 1107 4 0.25020425860888906 1107 5 0.30970948650855235 1107 6 0.43538470093106696 1107 7 0.4481502599953302 1107 8 0.17214621846621725 1107 9 0.4225363401234432 1107 10-11 0.3097745773984357 1107 12-13 0.39151010403173103 1107 14-15 0.44338981794534504 1107 16-17 0.5114004935769927 1107 18-19 0.22113070870137363 1107 20-21 0.5062037515091333 1107 22-23 0.627837754898799 1107 24-25 0.9265786417337338 1107 26-27 0.6218653050018972 1107 28-29 0.3908432717676291 1107 30-31 0.45049331174507756 1107 32-33 0.5983468014178044 1107 34-35 0.43348661621299556 1107 36-37 0.7827864913356848 1107 38-39 0.38861716459298634 1107 40-41 0.7053663787521813 1107 42-43 0.483280405785635 1107 44-45 1.3143374624868045 1107 46-47 1.1509135877709866 1107 48-49 0.9972139982324677 1107 50-51 1.4296531547308433 1107 52-53 1.0585567385886954 1107 54-55 0.6504487582249787 1107 56-57 1.1291075365257335 1107 58-59 0.9355894533737086 1107 60-61 1.096191745258345 1107 62-63 1.0394790413944968 1107 64-65 1.2662798757003735 1107 66-67 1.158758937878165 1107 68-69 0.6193385434475935 1107 70-71 0.11950575776267414 1107 72-73 0.4934702705115832 1107 74-75 0.8399588523624679 1107 76-77 0.5098232672969374 1107 78-79 0.6357417399887204 1107 80-81 0.8688436108900781 1107 82-83 0.8305821117958097 1107 84-85 1.236976898485814 1107 86-87 1.3062224758726693 1107 88-89 1.2720266092018342 1107 90-91 0.7948243814742391 1107 92-93 0.8842365177694553 1107 94-95 1.3725898866592487 1107 96-97 1.3206807190647183 1107 98-99 1.552208019390676 1107 100-101 1.8993200946268622 1108 1 0.1756746648515275 1108 2 0.3335241428626219 1108 3 0.13446941865672812 1108 4 0.08976680441192286 1108 5 0.20694157399939428 1108 6 0.05633289417758647 1108 7 0.16825849608530064 1108 8 0.08719216243149219 1108 9 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60-61 -0.46088588031243205 2316 62-63 -0.5678268946785607 2316 64-65 -0.6971904439343284 2316 66-67 -0.33439174705334196 2316 68-69 -0.5336294930820848 2316 70-71 -0.5951061143834444 2316 72-73 -0.5384931998080518 2316 74-75 -0.173739777774518 2316 76-77 -0.0358388331596835 2316 78-79 -0.5126600864952984 2316 80-81 -0.4667728274660874 2316 82-83 -0.7561758790717725 2316 84-85 -0.6808313206922669 2316 86-87 -0.931220446501758 2316 88-89 -0.8512610620310426 2316 90-91 -0.2508377189823854 2316 92-93 -0.4445306055182172 2316 94-95 -0.026953492336186713 2316 96-97 -0.4190453083325423 2316 98-99 -0.593910702070513 2316 100-101 -0.831273512679072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 25.0 6 46.0 7 106.0 8 153.0 9 294.0 10 510.0 11 938.0 12 1595.0 13 2170.0 14 2564.0 15 3679.0 16 5358.0 17 7687.0 18 6210.0 19 3304.0 20 2599.0 21 2546.0 22 3000.0 23 3178.0 24 3553.0 25 3877.0 26 4201.0 27 4940.0 28 5821.0 29 6814.0 30 7653.0 31 9314.0 32 11296.0 33 14170.0 34 19415.0 35 31325.0 36 31411.0 37 32306.0 38 20425.0 39 1451.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.11878438894513 18.692619448043892 14.624040457350814 13.564555705660169 2 14.60979893363156 26.468619312767284 38.46998968284596 20.4515920707552 3 23.93107196018051 34.31989478078016 29.85319713639908 11.895836122640247 4 10.88149008643604 18.30120695426175 36.14365315324184 34.67364980606037 5 9.970539350447817 46.20516900487598 28.74146310723204 15.08282853744417 6 23.144290518456682 37.12364045899522 18.676805305064857 21.055263717483243 7 23.06592740188859 42.77444811101573 17.687224843076873 16.472399644018807 8 27.61689493041828 30.130027643672275 19.215896292912664 23.03718113299678 9 31.126302442251504 18.805572838319957 20.407094421648697 29.661030297779845 10-11 24.32780984933805 29.728170555944967 22.412442015231584 23.5315775794854 12-13 32.52138334068427 28.607601915443258 24.280330476970573 14.590684266901896 14-15 27.837414253423958 27.34892457451584 30.67994770541769 14.133713466642513 16-17 16.658856607310216 32.01566474762351 32.17140651949627 19.154072125570003 18-19 16.22805635843841 29.938057697305727 38.95178502516283 14.882100919093036 20-21 19.190300300063793 27.838398714687372 36.80644703992188 16.16485394532696 22-23 17.360982582705567 28.521361092843673 35.66095541125371 18.456700913197054 24-25 17.795318689800194 29.052239452482027 37.48552841942775 15.666913438290031 26-27 19.754474394061706 26.78946228514048 38.719604638799744 14.736458681998071 28-29 16.8478421381134 27.574243877082893 40.82345088572852 14.754463099075188 30-31 15.86695596701661 32.4358328148504 38.29514936246289 13.402061855670103 32-33 14.813779307411812 32.13990375906689 37.054137493797896 15.992179439723406 34-35 15.825805486205729 29.491899852724597 37.504036291180014 17.17825836988966 36-37 18.06230458443921 30.104037866318034 37.51427468831957 14.319382860923188 38-39 20.094429524387074 27.78681294448426 38.148464634213575 13.970292896915094 40-41 15.93793131963874 30.40526571750914 39.368050893010086 14.288752069842031 42-43 15.041787118067315 33.19023575653982 36.853599363543985 14.914377761848874 44-45 15.92700810408512 33.46242902034291 33.94363368590172 16.666929189670245 46-47 15.578115032329709 32.574248068487 31.997156875871248 19.85048002331204 48-49 17.27591692722075 32.00168539768297 32.30844352736408 18.413954147732195 50-51 19.232632134390776 33.49944476384743 31.554739984091107 15.713183117670686 52-53 16.259756011120473 34.11926157529553 32.527978389106345 17.093004024477647 54-55 14.115599379395618 33.63234703440889 33.1279090830334 19.12414450316209 56-57 17.27060083639829 35.38035645373426 28.5999779480677 18.74906476179975 58-59 16.52871082828633 33.20056232427366 30.297976735211424 19.972750112228585 60-61 17.216849251415656 31.82625440054185 31.36670788277823 19.59018846526427 62-63 21.228922684350216 31.3405212131713 30.62757436620384 16.802981736274642 64-65 19.61243728981752 32.257054649413654 32.240712592440914 15.889795468327913 66-67 17.223740480259583 32.61559544155057 32.75893300150426 17.401731076685596 68-69 17.280051664527104 34.666622037756056 31.386003323541225 16.66732297417561 70-71 17.084340765359567 34.825907870177126 29.01364857095603 19.07610279350728 72-73 16.944744158206863 32.970395280886486 28.71397856237153 21.370881998535122 74-75 17.631898120072773 33.649870444897736 29.913052381214904 18.80517905381459 76-77 18.31255463760012 31.62837768659479 32.25016342056973 17.808904255235365 78-79 16.287320926496186 30.930000866325912 34.32087924204358 18.46179896513432 80-81 15.16030967213502 35.11908043442307 32.06764430233199 17.652965591109922 82-83 14.938262610829 34.163980648857894 30.17226611955257 20.72549062076054 84-85 16.125373851373038 31.384416673722015 30.033334055275855 22.456875419629093 86-87 17.41590731887882 31.25546376001197 30.97843636048609 20.350192560623125 88-89 18.672276783253132 28.583438998842276 32.63666291258772 20.10762130531688 90-91 15.89442052986653 30.149106189915443 33.49848176343866 20.45799151677937 92-93 14.204339607781366 32.82684886193589 32.56536977238718 20.403441757895564 94-95 14.323320705976862 32.97708961747773 30.645491561198053 22.054098115347358 96-97 14.439684027312893 31.111929307805596 29.329463744260593 25.118922920620918 98-99 16.62518705205954 29.917893990706467 29.759588879262814 23.697330077971174 100-101 17.96918386879512 30.31316341923566 30.82971960629084 20.88793310567838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3420.0 1 2258.0 2 1433.5 3 1758.5 4 1431.5 5 1362.5 6 2614.5 7 4928.0 8 6331.5 9 6129.5 10 4814.0 11 3521.5 12 3133.5 13 2933.0 14 2586.5 15 2134.5 16 1761.0 17 1524.5 18 1403.5 19 1343.0 20 1312.0 21 1276.5 22 1342.5 23 1413.0 24 1446.0 25 1570.0 26 1709.0 27 1853.5 28 1993.5 29 2171.5 30 2500.5 31 2737.5 32 3003.5 33 3450.0 34 3928.0 35 4127.5 36 4401.0 37 4870.0 38 4931.5 39 4818.0 40 4781.0 41 4770.5 42 4755.5 43 4844.5 44 5363.0 45 5437.0 46 5751.0 47 8539.0 48 25275.5 49 25327.0 50 11139.5 51 8234.0 52 3655.0 53 2144.0 54 1789.0 55 1702.5 56 1923.0 57 2176.0 58 2245.5 59 2513.0 60 2661.0 61 2677.5 62 2375.0 63 1260.0 64 469.5 65 339.5 66 335.0 67 267.5 68 184.0 69 156.0 70 152.5 71 130.0 72 101.5 73 132.5 74 131.5 75 74.0 76 48.0 77 34.5 78 27.0 79 17.5 80 14.5 81 12.0 82 6.0 83 3.0 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019295440762996857 2 0.0 3 0.0 4 3.9378450536728285E-4 5 0.018901656257629573 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003937845053672828 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011813535161018483 24-25 0.0 26-27 3.9378450536728285E-4 28-29 9.84461263418207E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.906767580509242E-4 42-43 0.015751380214691312 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0029533837902546213 84-85 5.906767580509242E-4 86-87 0.0 88-89 0.0 90-91 0.012994888677120333 92-93 0.0023627070322036967 94-95 0.0 96-97 0.0 98-99 0.0023627070322036967 100-101 0.02087057878446599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 253946.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.37302418624432 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00656854397275 24.605624817874666 2 11.945018854153997 7.733927685413435 3 4.355917771560637 4.230426941160719 4 2.317236345943316 3.0006379308986952 5 1.301544824230629 2.106747103714963 6 0.8125532173701495 1.5782882975120693 7 0.5619754287799538 1.2734990903577925 8 0.43911932854883834 1.1372496515007129 9 0.2809877143899769 0.818677986658581 >10 1.6226736406763167 9.636300630842777 >50 0.17029558447877388 3.87956494687847 >100 0.13988565867899283 9.475636552652926 >500 0.017029558447877385 3.7527663361502053 >1k 0.023111543607833594 14.03447977128996 >5k 0.0060819851599562096 12.736172257094028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7737 3.046710718026667 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6708 2.641506462003733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6571 2.5875579847684156 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5970 2.3508934970426782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5357 2.109503595252534 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4665 1.8370047175383744 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3380 1.3309916281414158 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2194 0.8639632047758184 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2160 0.850574531593331 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2151 0.8470304710450254 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.8281288147873958 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1990 0.7836311656808929 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.7466154221763682 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1781 0.7013302040591307 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1622 0.6387184677057327 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1597 0.6288738550715507 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1529 0.6020965087065755 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1387 0.5461791089444212 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1376 0.5418474793853811 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1280 0.504044166870122 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1272 0.5008938908271837 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1143 0.45009568963480423 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1108 0.4363132319469494 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1006 0.3961472123994865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 987 0.3886653067975081 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 958 0.37724555614185695 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 954 0.3756704181203878 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 838 0.329991415497783 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.2980948705630331 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 607 0.23902719475794065 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 594 0.233907996188166 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 584 0.22997015113449315 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 577 0.2272136595969222 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 572 0.22524473707008574 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 569 0.22406338355398392 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 513 0.20201145125341607 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 510 0.20083009773731422 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 510 0.20083009773731422 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 499 0.19649846817827413 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 471 0.1854725020279902 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.18074708796358283 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 451 0.17759681192064455 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 429 0.16893355280256434 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 416 0.16381435423278964 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 404 0.15908894016838226 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 402 0.1583013711576477 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 394 0.15515109511470943 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 391 0.15396974159860757 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 385 0.1516070345664039 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 382 0.15042568105030205 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 355 0.1397934994053854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 328 0.12916131776046874 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 322 0.12679861072826507 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 313 0.12325455017995952 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 312 0.12286076567459224 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 298 0.11734778259945028 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 296 0.11656021358871571 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 294 0.11577264457798114 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.11537886007261386 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.11537886007261386 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 289 0.11380372205114474 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 280 0.1102596615028392 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 268 0.1055342474384318 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 266 0.10474667842769723 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 263 0.10356532491159538 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 262 0.10317154040622808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 261 0.10277775590086081 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 259 0.10199018689012625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.937845053672828E-4 0.0 20-21 0.0 0.0 0.0 5.906767580509242E-4 0.0 22-23 0.0 0.0 0.0 9.84461263418207E-4 0.0 24-25 0.0 0.0 0.0 0.0023627070322036967 0.0 26-27 0.0 0.0 0.0 0.006497444338560167 0.0 28-29 0.0 0.0 0.0 0.013388673182487616 0.0 30-31 0.0 0.0 0.0 0.021855040047884196 0.0 32-33 0.0 0.0 0.0 0.030124514660597138 0.0 34-35 0.0 0.0 0.0 0.04371008009576839 0.0 36-37 0.0 0.0 0.0 0.05434226174068503 0.0 38-39 0.0 0.0 0.0 0.0695029651973254 0.0 40-41 0.0 0.0 0.0 0.09214557425594418 0.0 42-43 0.0 0.0 0.0 0.12286076567459223 0.0 44-45 0.0 0.0 0.0 0.16440503099084058 0.0 46-47 0.0 0.0 0.0 0.254778574972632 0.0 48-49 0.0 0.0 0.0 0.32605357044411015 0.0 50-51 0.0 0.0 0.0 0.39142179833507906 0.0 52-53 0.0 0.0 0.0 0.4737227599568412 0.0 54-55 0.0 0.0 0.0 0.5607491356430108 0.0 56-57 0.0 0.0 0.0 0.6406873902325692 0.0 58-59 0.0 0.0 0.0 0.7891441487560348 0.0 60-61 0.0 0.0 0.0 1.0049380576973057 0.0 62-63 0.0 0.0 0.0 1.1992707110960596 0.0 64-65 0.0 0.0 0.0 1.3258724295716413 0.0 66-67 0.0 0.0 0.0 1.5107542548415807 0.0 68-69 0.0 0.0 0.0 1.7111905680735275 0.0 70-71 0.0 0.0 0.0 1.8868184574673355 0.0 72-73 0.0 0.0 0.0 2.1866853583045214 0.0 74-75 0.0 0.0 0.0 2.422956061524891 0.0 76-77 0.0 0.0 0.0 2.606656533278729 0.0 78-79 0.0 0.0 0.0 2.8492277885849746 0.0 80-81 0.0 0.0 0.0 3.072503603128224 0.0 82-83 0.0 0.0 0.0 3.357406692761453 0.0 84-85 0.0 0.0 0.0 3.8575130145779024 0.0 86-87 0.0 0.0 0.0 4.46512250635962 0.0 88-89 0.0 0.0 0.0 4.939632835327195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 50 6.548362E-11 76.01418 1 CATATAG 25 0.005013079 57.010635 1 GTCACTA 90 2.237357E-10 47.508865 2 AGTACTC 80 2.249999E-7 41.57026 5 AAGTACT 60 8.2634084E-5 39.590717 4 GGCAGCC 60 8.2730076E-5 39.582924 7 GTATAAT 50 0.0016119668 38.00709 1 GGCCCGT 25 0.001615049 37.999603 82-83 ATATAGG 65 1.3238171E-4 36.54528 2 TAATGGA 55 0.0025735006 34.551903 4 TCACTAC 125 5.6043064E-9 34.206383 3 ACTACCT 145 6.5847416E-10 32.764732 5 ATGGGTA 75 3.0719332E-4 31.66634 7 GATACTT 30 0.003949094 31.666338 38-39 GACATGG 60 0.0039454075 31.666338 7 TAAGCAC 30 0.003949094 31.666338 88-89 CAGCCAT 95 3.4804987E-5 29.999691 9 GGGACTC 285 0.0 29.99969 7 CATGAGA 80 4.4789934E-4 29.693043 4 GTGTAAG 80 4.4789934E-4 29.693043 1 >>END_MODULE