##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 237045 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87838596047164 33.0 31.0 34.0 30.0 34.0 2 31.86212322554789 33.0 31.0 34.0 30.0 34.0 3 31.780396127317598 33.0 31.0 34.0 30.0 34.0 4 35.450986099685714 37.0 35.0 37.0 33.0 37.0 5 35.43535193739585 37.0 35.0 37.0 33.0 37.0 6 35.616439916471556 37.0 35.0 37.0 33.0 37.0 7 35.61728785673606 37.0 35.0 37.0 33.0 37.0 8 35.642882153177666 37.0 35.0 37.0 33.0 37.0 9 37.179333038030755 39.0 37.0 39.0 34.0 39.0 10-11 37.08011980847519 39.0 37.0 39.0 33.5 39.0 12-13 36.89809951696935 39.0 37.0 39.0 33.0 39.0 14-15 37.59796241220022 40.0 37.0 41.0 32.5 41.0 16-17 37.6801050433462 39.5 37.0 41.0 32.5 41.0 18-19 37.97960724756902 40.0 37.5 41.0 33.5 41.0 20-21 38.12085469003776 40.0 38.0 41.0 34.0 41.0 22-23 37.98034972262651 40.0 37.5 41.0 33.5 41.0 24-25 37.74187179649434 40.0 37.0 41.0 33.0 41.0 26-27 37.46472399755321 39.5 36.0 41.0 33.0 41.0 28-29 37.35261659178637 39.0 36.0 41.0 33.0 41.0 30-31 36.8382712143264 38.5 35.0 40.0 33.0 41.0 32-33 36.25737729123162 38.0 35.0 40.0 32.0 41.0 34-35 36.007161087557215 38.0 35.0 40.0 31.0 41.0 36-37 35.652376131114345 38.0 35.0 40.0 31.0 41.0 38-39 35.29113459469721 37.0 35.0 40.0 30.0 41.0 40-41 34.78053534139087 36.5 35.0 40.0 28.5 41.0 42-43 34.30377354510747 36.0 35.0 40.0 24.5 41.0 44-45 33.67026513953047 36.0 34.5 40.0 21.0 41.0 46-47 32.631162859372694 36.0 33.0 40.0 5.5 41.0 48-49 31.78671982113101 35.0 33.0 40.0 2.0 41.0 50-51 30.658252652449956 35.0 30.5 39.0 2.0 40.0 52-53 30.62883629690565 35.0 31.0 39.0 2.0 40.0 54-55 30.896414182961042 35.0 31.0 39.0 2.0 41.0 56-57 30.728861608555334 35.0 31.0 39.0 2.0 41.0 58-59 30.537049927229006 35.0 31.0 39.0 2.0 41.0 60-61 30.127102448902107 35.0 30.5 39.0 2.0 41.0 62-63 29.586538420974918 35.0 29.0 38.5 2.0 40.0 64-65 29.079693729038787 35.0 28.0 38.0 2.0 40.0 66-67 28.618610390432195 35.0 27.5 37.0 2.0 40.0 68-69 28.203081693349365 35.0 26.0 37.0 2.0 39.0 70-71 27.68567993418971 35.0 26.0 36.0 2.0 39.0 72-73 27.11624375118648 34.0 24.5 35.5 2.0 38.5 74-75 26.651631546752725 34.0 22.5 35.0 2.0 37.0 76-77 26.036406589466136 33.5 19.0 35.0 2.0 37.0 78-79 25.543335653567887 33.0 15.0 35.0 2.0 36.0 80-81 25.044156172878566 33.0 6.5 35.0 2.0 36.0 82-83 24.536003712375287 33.0 2.0 35.0 2.0 35.5 84-85 24.053470859963298 32.0 2.0 35.0 2.0 35.0 86-87 23.417897445632686 32.0 2.0 35.0 2.0 35.0 88-89 22.756468181147042 31.0 2.0 35.0 2.0 35.0 90-91 22.087825096500666 30.5 2.0 34.5 2.0 35.0 92-93 21.53153198759729 30.0 2.0 34.0 2.0 35.0 94-95 20.925090172752007 29.5 2.0 34.0 2.0 35.0 96-97 20.070149127802736 29.0 2.0 34.0 2.0 35.0 98-99 19.139758273745493 27.0 2.0 34.0 2.0 35.0 100-101 17.436062772891223 21.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29148927401189795 1101 2 0.43372078099409705 1101 3 0.5028407601456308 1101 4 0.5222782897239284 1101 5 0.4168729328901577 1101 6 0.45168950072477543 1101 7 0.5418477507700317 1101 8 0.3903781045626289 1101 9 0.5135610958201866 1101 10-11 0.641854224810011 1101 12-13 0.628779374742777 1101 14-15 0.7444619855929488 1101 16-17 0.865050102541737 1101 18-19 0.6616603326185739 1101 20-21 0.5621601849005202 1101 22-23 0.6544680466182484 1101 24-25 0.3701068619100809 1101 26-27 0.6728449818227276 1101 28-29 0.3567171174448802 1101 30-31 0.7484065683867769 1101 32-33 0.8386795535072125 1101 34-35 0.6340577680438528 1101 36-37 1.0344473029606718 1101 38-39 0.9674258222791323 1101 40-41 0.31702709093524817 1101 42-43 0.32538287532695165 1101 44-45 0.3121870701702676 1101 46-47 -0.12033172571414497 1101 48-49 0.06902475364973171 1101 50-51 -0.04243633371520872 1101 52-53 0.2520387322189457 1101 54-55 0.07649025857624636 1101 56-57 0.09121592847191096 1101 58-59 0.36869977871150184 1101 60-61 0.49481332812909784 1101 62-63 0.806134878889825 1101 64-65 0.35058383607533017 1101 66-67 0.7974813731863151 1101 68-69 0.9798315165544231 1101 70-71 0.9069664131882682 1101 72-73 0.789390483392836 1101 74-75 0.5151016439037193 1101 76-77 0.9316978117945176 1101 78-79 0.7406671586638183 1101 80-81 0.4235906171384123 1101 82-83 0.8307970599176819 1101 84-85 1.0080363116267712 1101 86-87 1.0150931112775687 1101 88-89 0.891489982710933 1101 90-91 0.8347049299849658 1101 92-93 0.4558659428201999 1101 94-95 0.8887162168126146 1101 96-97 1.0702736226282674 1101 98-99 0.81686777300175 1101 100-101 1.7165098650744817 1102 1 0.1052774516868844 1102 2 0.14746474398981135 1102 3 0.2616666903544598 1102 4 0.166384132863854 1102 5 0.029314263411890806 1102 6 0.23406825046969004 1102 7 0.068801699481007 1102 8 0.23033797123836308 1102 9 0.2020686803382432 1102 10-11 0.1495492795681912 1102 12-13 0.42988134059543626 1102 14-15 0.4924043024421394 1102 16-17 0.5283468170509522 1102 18-19 0.6087217570114731 1102 20-21 0.31206665384821264 1102 22-23 0.6302435040701226 1102 24-25 0.39962016032515635 1102 26-27 0.20251133105066543 1102 28-29 0.4445852896780522 1102 30-31 0.20635160613930736 1102 32-33 -0.07012767049115354 1102 34-35 0.1527129615681062 1102 36-37 0.3647819740714766 1102 38-39 0.4463813355095283 1102 40-41 0.4379542387483255 1102 42-43 0.539756047220024 1102 44-45 0.735083471775603 1102 46-47 0.6805916808568355 1102 48-49 0.20638785820043992 1102 50-51 0.20464719801732656 1102 52-53 0.001724127770266648 1102 54-55 -0.5650307263907628 1102 56-57 -0.2116121104370059 1102 58-59 -0.1359682710810297 1102 60-61 -0.26686104776369746 1102 62-63 0.06783476325143312 1102 64-65 -0.24022631289412644 1102 66-67 -0.22957460939990426 1102 68-69 -0.2798446959481282 1102 70-71 -0.20968381468738784 1102 72-73 -0.007570607689405762 1102 74-75 -0.04124656370120405 1102 76-77 0.2622280478328811 1102 78-79 0.2255661451273241 1102 80-81 0.16231587392366364 1102 82-83 0.24269931146519852 1102 84-85 0.5917721119124693 1102 86-87 0.8142139193858604 1102 88-89 1.2292221097771225 1102 90-91 1.5068785916406355 1102 92-93 1.386287342724966 1102 94-95 1.886675539307685 1102 96-97 2.3627707316684656 1102 98-99 2.400595070464508 1102 100-101 2.992715565366975 1103 1 0.25783130617814365 1103 2 0.17635649307529988 1103 3 0.2372120094169219 1103 4 0.17126152075167766 1103 5 0.1860680908065575 1103 6 0.23254406675474826 1103 7 0.36357882995113044 1103 8 0.4151029082387723 1103 9 0.5025360966774954 1103 10-11 0.3889307996874152 1103 12-13 0.30022411257755977 1103 14-15 0.679032130649702 1103 16-17 0.7429349487404195 1103 18-19 0.2664578361335401 1103 20-21 0.4808615325909287 1103 22-23 0.608447676946426 1103 24-25 0.7147366757024756 1103 26-27 0.4679394573208171 1103 28-29 0.4489207455783415 1103 30-31 0.7852365810749546 1103 32-33 0.8817250594912309 1103 34-35 0.9144322407985541 1103 36-37 0.8975349885681609 1103 38-39 1.1008488873317717 1103 40-41 1.4120769863435 1103 42-43 1.153629506100934 1103 44-45 1.5045930233268834 1103 46-47 1.8671687029405604 1103 48-49 2.1131247502204893 1103 50-51 1.2692556521563283 1103 52-53 1.1699770745254519 1103 54-55 1.1859515253366446 1103 56-57 1.3544527859185003 1103 58-59 1.3795275966875629 1103 60-61 1.1761514166720168 1103 62-63 1.8562103221187982 1103 64-65 1.8675842818762298 1103 66-67 2.225716001628413 1103 68-69 2.0227227068428206 1103 70-71 2.090902149274136 1103 72-73 2.3707148549850316 1103 74-75 2.5664116126976566 1103 76-77 2.8554572850636113 1103 78-79 2.611624944747966 1103 80-81 2.8144294086750534 1103 82-83 2.7777724722835124 1103 84-85 2.7269502720533687 1103 86-87 2.730637845683116 1103 88-89 3.2446671714218844 1103 90-91 3.2796227997425156 1103 92-93 3.4917100496752447 1103 94-95 3.4830716915905704 1103 96-97 3.774639042195499 1103 98-99 3.6748973330305574 1103 100-101 3.641983957183804 1104 1 -0.14353486012308636 1104 2 -0.1912940382115913 1104 3 0.0043565531848379635 1104 4 -0.36663694441951833 1104 5 -0.09096007583200105 1104 6 -0.05089327512415309 1104 7 0.008932177901883165 1104 8 0.10031120643408542 1104 9 0.3638786502278393 1104 10-11 0.37520167167257057 1104 12-13 0.30616103280019047 1104 14-15 0.30023300769473593 1104 16-17 0.10910124997166548 1104 18-19 0.3655133260981174 1104 20-21 0.04094755046915566 1104 22-23 0.1869263411393689 1104 24-25 -0.015192486044867337 1104 26-27 0.042850909504998924 1104 28-29 0.11081145227424116 1104 30-31 0.17351453688537788 1104 32-33 0.21487567510937566 1104 34-35 0.07039446039714647 1104 36-37 0.2404427301772003 1104 38-39 -0.042151618655744016 1104 40-41 0.13746912667070177 1104 42-43 0.7406593593645567 1104 44-45 1.1376930401931347 1104 46-47 1.4833434372971155 1104 48-49 1.404489229896658 1104 50-51 1.2462079206670431 1104 52-53 1.3604645091687289 1104 54-55 1.0640759982663148 1104 56-57 0.6924946142200703 1104 58-59 0.4502562187148875 1104 60-61 0.7337327963313172 1104 62-63 0.991739079076126 1104 64-65 1.1871406994845977 1104 66-67 1.266996150051419 1104 68-69 1.022764872469402 1104 70-71 0.9622716888254956 1104 72-73 1.1964190209489338 1104 74-75 1.3872161328528279 1104 76-77 1.3855719755679097 1104 78-79 1.3777575134779418 1104 80-81 0.6963740873729769 1104 82-83 0.6192550157141099 1104 84-85 0.7974596328224699 1104 86-87 0.8320208782349781 1104 88-89 0.6256757732097071 1104 90-91 0.2436049215168481 1104 92-93 0.6770785772515673 1104 94-95 0.3553941743026634 1104 96-97 0.7307867905510363 1104 98-99 0.7212457897686271 1104 100-101 1.2220564820615216 1105 1 0.5170943915775901 1105 2 0.42239643797888604 1105 3 0.5165164171304184 1105 4 0.497053531770959 1105 5 0.26210114865779843 1105 6 0.1018278133112176 1105 7 0.22576891259576115 1105 8 0.3029472608558521 1105 9 0.5371779699557351 1105 10-11 0.41980201180862764 1105 12-13 0.4621559307593728 1105 14-15 0.35627042266072095 1105 16-17 0.1965162159580558 1105 18-19 0.3080728499076386 1105 20-21 0.14413632597936044 1105 22-23 0.03628858603732965 1105 24-25 0.06635513300275164 1105 26-27 -0.10804194763785091 1105 28-29 0.20332845907696395 1105 30-31 0.4737696389261927 1105 32-33 0.61819200163999 1105 34-35 1.0075968413495175 1105 36-37 0.6287505533064532 1105 38-39 0.6016408983510573 1105 40-41 0.7378517797319333 1105 42-43 1.1506508559631925 1105 44-45 1.459844400737346 1105 46-47 1.4172720087256891 1105 48-49 1.3641405904408792 1105 50-51 1.545357580530986 1105 52-53 1.491034237059882 1105 54-55 1.3848668145928436 1105 56-57 2.004952097213259 1105 58-59 2.1161150622523017 1105 60-61 1.805714088848326 1105 62-63 1.9556421403006148 1105 64-65 2.2938583865594246 1105 66-67 1.9427614561738658 1105 68-69 2.3574162330136232 1105 70-71 2.4964978793016854 1105 72-73 2.1698884087040398 1105 74-75 1.996697425369831 1105 76-77 1.9960736762481837 1105 78-79 1.7916180576956293 1105 80-81 1.810744835672299 1105 82-83 1.7321801857821377 1105 84-85 1.6018814015299547 1105 86-87 1.2019188374186491 1105 88-89 0.9228696507607275 1105 90-91 1.2985794113127653 1105 92-93 1.7718064684080268 1105 94-95 1.9456577797448453 1105 96-97 1.6642051578979746 1105 98-99 1.5975212999727049 1105 100-101 2.1583234888642444 1106 1 0.2206796628954244 1106 2 0.3701677558083425 1106 3 0.09975285123894295 1106 4 0.125057407739952 1106 5 0.036519753308958514 1106 6 -0.09313342699885396 1106 7 0.03797821492133835 1106 8 0.029594547677554317 1106 9 0.01760432654487687 1106 10-11 -0.06328907346268409 1106 12-13 0.11840593075937278 1106 14-15 0.22671615909483478 1106 16-17 -0.1775147917938824 1106 18-19 0.1319585088223718 1106 20-21 0.31346772132819467 1106 22-23 0.2640502139443086 1106 24-25 0.010056683390345711 1106 26-27 0.1275685174784229 1106 28-29 0.49790210248782074 1106 30-31 0.656618476135499 1106 32-33 0.4301106062911586 1106 34-35 0.724215058403793 1106 36-37 0.6458532664847496 1106 38-39 0.558133146413077 1106 40-41 0.6818924774063504 1106 42-43 0.44246287146707175 1106 44-45 0.8302416875590595 1106 46-47 0.8059347994233619 1106 48-49 0.648200667960257 1106 50-51 0.5940009913837017 1106 52-53 0.37451291923042973 1106 54-55 0.48472146575563 1106 56-57 0.5154656631047381 1106 58-59 0.32391544984920273 1106 60-61 0.5111888950498766 1106 62-63 1.1592758612308494 1106 64-65 0.7902731152415967 1106 66-67 0.74886610733666 1106 68-69 1.0385693337888142 1106 70-71 0.9868564064334677 1106 72-73 0.8350046877738038 1106 74-75 0.9251373478504519 1106 76-77 0.8140484824497314 1106 78-79 0.8333816235871012 1106 80-81 0.5577409597033061 1106 82-83 0.4872189454720619 1106 84-85 0.05033101393305728 1106 86-87 0.3350583723023739 1106 88-89 0.6068812786677036 1106 90-91 0.6490154578243938 1106 92-93 1.1836281738343821 1106 94-95 1.3901829735432933 1106 96-97 1.594147018363092 1106 98-99 2.313509672065731 1106 100-101 2.9114727136704452 1107 1 0.05930453650513101 1107 2 0.03417180029772382 1107 3 0.23372656205795295 1107 4 -0.08899719286672081 1107 5 -0.12485537308133132 1107 6 0.0532770886735392 1107 7 0.14859499955228017 1107 8 0.19117189853701433 1107 9 0.19333739024558838 1107 10-11 0.4496027364463089 1107 12-13 0.3118841916289412 1107 14-15 0.32565448063174074 1107 16-17 0.6197046217551545 1107 18-19 0.3183083571540166 1107 20-21 0.6117993694576214 1107 22-23 0.32840815125472034 1107 24-25 0.6357391909737657 1107 26-27 0.45962109584040434 1107 28-29 0.8439081692218977 1107 30-31 1.1308348563174988 1107 32-33 0.9861267842486896 1107 34-35 0.8151149572915557 1107 36-37 0.6994933069296394 1107 38-39 0.444937999800338 1107 40-41 1.032145257992802 1107 42-43 0.8697631748037722 1107 44-45 0.4005146905924235 1107 46-47 0.1134676608996017 1107 48-49 0.5716587063829017 1107 50-51 0.7544155515454811 1107 52-53 0.360508874741047 1107 54-55 0.10307333633197047 1107 56-57 0.34453543054659974 1107 58-59 0.32816216370157747 1107 60-61 0.33080466855847135 1107 62-63 0.5830877924745259 1107 64-65 0.40382215467536753 1107 66-67 0.20417449965212597 1107 68-69 0.07036913156434821 1107 70-71 0.1846319372726981 1107 72-73 0.22206232174751506 1107 74-75 -0.037451125354806436 1107 76-77 0.38801208204528237 1107 78-79 0.48419052146374497 1107 80-81 0.2957086037882384 1107 82-83 0.2118903307096751 1107 84-85 0.36564951747198293 1107 86-87 0.4530057939403882 1107 88-89 1.0125435638042042 1107 90-91 0.8852641939214614 1107 92-93 1.1018789321761453 1107 94-95 1.0401324174260047 1107 96-97 1.365835592680586 1107 98-99 1.1187169521466167 1107 100-101 1.6377618946613488 1108 1 0.10023595364175009 1108 2 -0.03253731376449309 1108 3 0.14955337668411417 1108 4 0.19965116078071787 1108 5 0.2692141193690958 1108 6 0.40594217872265403 1108 7 0.46771451929031826 1108 8 0.4825672050678449 1108 9 0.28484184722211836 1108 10-11 0.3486112865645623 1108 12-13 0.2815858470773662 1108 14-15 0.5903534073190144 1108 16-17 0.4742707487335096 1108 18-19 0.42642187361754935 1108 20-21 0.15566875275759884 1108 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#Quality Count 2 1052.0 3 160.0 4 126.0 5 136.0 6 169.0 7 201.0 8 238.0 9 330.0 10 515.0 11 815.0 12 1524.0 13 2494.0 14 2659.0 15 3850.0 16 5586.0 17 7869.0 18 6240.0 19 3058.0 20 2786.0 21 2742.0 22 3061.0 23 3347.0 24 3613.0 25 3713.0 26 4136.0 27 4626.0 28 5444.0 29 6213.0 30 7388.0 31 8854.0 32 10746.0 33 13506.0 34 18128.0 35 30102.0 36 27081.0 37 25969.0 38 16635.0 39 1932.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.63047995497116 16.959876086045714 14.307237201271302 14.102406757711824 2 14.731929405890739 24.31697110482066 40.38808988859129 20.56300960069731 3 22.390604441398335 34.40116429112355 30.852445561351626 12.35578570612649 4 10.618956167141853 17.99803720843662 37.883569234934306 33.49943738948722 5 9.867268989670837 42.61477552470624 30.396585708364 17.121369777258924 6 22.134744995898625 38.132647797518885 18.93947722256518 20.79312998401732 7 21.626580646252318 43.507671234034994 18.099377253913833 16.76637086579885 8 25.73867672428373 29.039255150018604 19.689307580421474 25.53276054527619 9 30.88086056423787 17.849350517556324 20.422755564576146 30.847033353629662 10-11 23.56760482384182 28.47136478189537 22.900563232582922 25.060467161679888 12-13 33.052289751141124 27.872151949581287 24.01104442081273 15.064513878464858 14-15 28.085578681437163 25.6731816115768 32.092851178081034 14.148388528905004 16-17 16.3308795859777 30.858445133166178 32.89917085607736 19.911504424778762 18-19 15.324950425558004 28.113042412045004 41.58460634299171 14.977400819405275 20-21 18.320079817029605 25.28462296176984 39.71383396535907 16.681463255841482 22-23 16.712779291380357 25.975392978903738 38.63040220495142 18.681425524764485 24-25 17.237210324740655 26.121712222795257 40.84410588523745 15.796971567226642 26-27 18.962831117603162 24.058200174608977 41.87909702213475 15.099871685653103 28-29 15.964904862579282 24.88076109936575 44.33868921775898 14.815644820295985 30-31 15.335832017402003 29.536263685157348 41.90284727374966 13.225057023690992 32-33 14.022892274385937 29.409003843568048 40.222157387557665 16.34594649448835 34-35 14.897282716691402 26.8214930471878 40.862199447833824 17.419024788286976 36-37 17.35354111840367 27.189828466343258 40.94570743101134 14.510922984241734 38-39 19.37698562843621 25.251169446634552 41.19686231073777 14.17498261419147 40-41 15.285081415600008 27.52151637611809 43.00083698280381 14.192565225478097 42-43 14.446762679654421 30.48655458809752 40.09715448959014 14.969528242657923 44-45 15.080188808868014 31.14543060132623 37.23479139494918 16.539589194856585 46-47 14.732367452026532 29.81964766611566 35.178005977923114 20.26997890393469 48-49 16.21470216427155 29.5840150852137 35.250694435636284 18.95058831487847 50-51 18.630680304612742 30.449012038207673 34.5370131545009 16.38329450267868 52-53 15.471823377304355 30.895440023849645 35.83958998906891 17.793146609777086 54-55 14.096767306525631 30.349861437875337 36.32103290612654 19.232338349472492 56-57 16.58364839419344 32.5757783887622 31.79025716996803 19.050316047076326 58-59 15.782143860961595 29.94317329292699 33.45019896744035 20.82448387867107 60-61 16.91020140752795 28.449817190438953 34.64230614789611 19.99767525413699 62-63 21.277774020088604 27.926417870066622 33.33939260712233 17.456415502722447 64-65 19.777333026187037 28.953258110669882 35.70406595473537 15.565342908407706 66-67 17.588811673599132 29.462987386290624 35.930049710865376 17.018151229244868 68-69 17.049059746320676 31.827645170222162 34.804710040011244 16.318585043445914 70-71 16.823731557467998 31.23580022783039 32.49457370566765 19.445894509033963 72-73 16.574199125415575 29.814494260671808 32.14108327573926 21.47022333817335 74-75 17.099111438016248 29.92851768246802 34.286360446732786 18.686010432782947 76-77 17.924873319648825 28.06606615494217 35.68697005122178 18.322090474187227 78-79 15.904488087436313 27.775405365410226 38.2453544172674 18.074752129886054 80-81 15.031982041016493 31.33457907575495 36.002308287428285 17.631130595800272 82-83 14.420422502462554 30.63185129130856 33.81626173676668 21.13146446946221 84-85 15.809119597220961 27.933707169323185 33.77438370960003 22.48278952385582 86-87 17.50069223241254 27.82499519136263 34.83059857495239 19.84371400127244 88-89 18.82951761515324 25.35051692394845 35.9046010564653 19.91536440443301 90-91 16.24495317817659 26.696049210476254 37.39594563172469 19.663051979622466 92-93 14.778818675279437 29.537768233085888 36.2330267007119 19.450386390922773 94-95 14.762487555248354 30.081527655355945 34.63984730210467 20.51613748729103 96-97 15.08435028511284 28.0708280319394 33.04476842243197 23.80005326051578 98-99 16.956518063223395 27.09737146915344 33.398363192113564 22.5477472755096 100-101 19.23778406629288 26.3637189780482 35.113584249312616 19.284912706346304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1967.0 1 1372.0 2 1198.0 3 1575.0 4 1209.5 5 1096.0 6 2117.0 7 4642.0 8 7244.0 9 7344.0 10 4987.0 11 3055.0 12 2700.0 13 2622.0 14 2390.0 15 2022.5 16 1660.0 17 1493.0 18 1406.5 19 1332.0 20 1300.0 21 1278.5 22 1310.0 23 1395.0 24 1535.0 25 1629.5 26 1718.5 27 1898.5 28 2042.0 29 2208.5 30 2482.5 31 2722.5 32 2936.0 33 3313.0 34 3771.0 35 3920.5 36 4136.0 37 4509.5 38 4493.5 39 4423.5 40 4323.0 41 4271.0 42 4294.5 43 4323.5 44 4844.5 45 5002.0 46 5551.0 47 8604.5 48 22854.5 49 23063.5 50 10790.0 51 7385.0 52 3348.0 53 2051.0 54 1748.5 55 1669.0 56 1794.5 57 1852.5 58 1822.5 59 2015.0 60 2232.5 61 2334.5 62 2013.5 63 1073.0 64 418.0 65 304.5 66 271.0 67 219.0 68 162.0 69 130.0 70 123.5 71 108.5 72 87.0 73 125.5 74 126.5 75 64.0 76 42.0 77 34.0 78 27.5 79 22.0 80 17.5 81 11.0 82 6.0 83 3.5 84 1.5 85 1.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31723934274082977 2 0.2986774663038664 3 0.2860216414604822 4 0.2729439557889852 5 0.2645067392267291 6 0.22738298635280224 7 0.21599274399375645 8 0.22822670800902783 9 0.23160159463393026 10-11 0.23328903794638148 12-13 0.23012508173553542 14-15 0.226750195110633 16-17 0.2261174038684638 18-19 0.22442996055601258 20-21 0.212617857368854 22-23 0.20523529287687992 24-25 0.20544622329093631 26-27 0.2178911177202641 28-29 0.22991415132147902 30-31 0.2195785610327153 32-33 0.23033601214959182 34-35 0.2202113522748845 36-37 0.2119850661266848 38-39 0.211563205298572 40-41 0.20291505832225948 42-43 0.21704739606403844 44-45 0.21493809192347446 46-47 0.21430530068130527 48-49 0.2202113522748845 50-51 0.23117973380581747 52-53 0.23771857664156595 54-55 0.21451623109536166 56-57 0.19004830306481893 58-59 0.1892045814085933 60-61 0.19405598093189055 62-63 0.20797738825961315 64-65 0.19342318968972136 66-67 0.20122761500980826 68-69 0.20523529287687992 70-71 0.196376215486511 72-73 0.20059482376763907 74-75 0.20375877997848513 76-77 0.22126600434516655 78-79 0.22358623889978693 80-81 0.21346157902507962 82-83 0.2117741357126284 84-85 0.2060790145331055 86-87 0.20734459701744395 88-89 0.211563205298572 90-91 0.21514902233753086 92-93 0.20966483157206436 94-95 0.2107194836423464 96-97 0.19911831086924422 98-99 0.20544622329093631 100-101 0.19342318968972136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 237045.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.201290894134026 #Duplication Level Percentage of deduplicated Percentage of total 1 77.32713272852024 25.673606277289124 2 11.499072450509516 7.635680988841781 3 4.185408248837386 4.168828703410745 4 2.1206576707072244 2.81634288848109 5 1.1626134024548296 1.9300132886160855 6 0.7623694442326752 1.5186989812061003 7 0.5031638331935656 1.169398215528697 8 0.3672079489720719 0.9753422345968065 9 0.29224162028919215 0.8732519141935076 >10 1.4535844070036341 8.819422472526313 >50 0.14357957866382048 3.3057014490919445 >100 0.13722649996188152 9.57835010230125 >500 0.019059236105816876 4.564534160180556 >1k 0.02160046758659246 14.701006138075048 >5k 0.003811847221163376 7.878250965006645 >10k+ 0.001270615740387792 4.391571220654306 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10410 4.391571220654306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7424 3.1318947879094687 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5726 2.415575101773925 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5525 2.3307810753232507 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4728 1.994557995317345 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3945 1.664240966905018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3201 1.3503765107890908 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2577 1.0871353540467 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2219 0.9361091775823156 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2113 0.8913919298023583 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1838 0.7753802020713366 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1793 0.7563964648062604 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1551 0.6543061444029614 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1549 0.6534624227467358 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1513 0.6382754329346749 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1486 0.6268851905756291 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1438 0.6066358708262144 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1394 0.588073994389251 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1226 0.5172013752662996 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1191 0.5024362462823515 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1086 0.45814085933050686 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 956 0.4032989516758422 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 945 0.39865848256660125 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 936 0.3948617351135861 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 0.3640658946613512 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 797 0.33622308000590606 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 731 0.3083802653504609 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 715 0.301630492100656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 695 0.29319327553839986 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 641 0.2704127908203084 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 629 0.2653504608829547 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 627 0.2645067392267291 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 605 0.2552258010082474 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 576 0.242991836992976 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 563 0.23750764622750956 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 541 0.22822670800902783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 486 0.20502436246282352 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 481 0.20291505832225948 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 470 0.19827458921301863 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.19447784176000338 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 459 0.19363412010377776 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 454 0.19152481596321375 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 425 0.1792908519479424 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 412 0.1738066611824759 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 386 0.16283827965154296 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 376 0.15861967137041488 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 372 0.15693222805796367 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 365 0.15397920226117404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 359 0.1514480372924972 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 358 0.1510261764643844 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 352 0.14849501149570757 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 348 0.14680756818325633 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 328 0.13837035162100023 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.13288616085553376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 302 0.1274019700900673 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 295 0.12444894429327764 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 291 0.12276150098082643 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.12191777932460081 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.11558986690290873 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 274 0.11558986690290873 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 273 0.11516800607479591 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 269 0.1134805627623447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 261 0.11010567613744225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 257 0.10841823282499102 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 252 0.106308928684427 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 249 0.10504334620008858 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 247 0.10419962454386299 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 247 0.10419962454386299 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 239 0.10082473791896054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.2186082811280557E-4 0.0 18-19 0.0 0.0 0.0 4.2186082811280557E-4 0.0 20-21 0.0 0.0 0.0 8.437216562256111E-4 0.0 22-23 0.0 0.0 0.0 0.0016874433124512223 0.0 24-25 0.0 0.0 0.0 0.0037967474530152504 0.0 26-27 0.0 0.0 0.0 0.007804425320086903 0.0 28-29 0.0 0.0 0.0 0.013710476913666182 0.0 30-31 0.0 0.0 0.0 0.023202345546204307 0.0 32-33 0.0 0.0 0.0 0.03311607500685523 0.0 34-35 0.0 0.0 0.0 0.039865848256660126 0.0 36-37 0.0 0.0 0.0 0.051045160201649474 0.0 38-39 0.0 0.0 0.0 0.06264633297475163 0.0 40-41 0.0 0.0 0.0 0.08648146976312515 0.0 42-43 0.0 0.0 0.0 0.11221498027800628 0.0 44-45 0.0 0.0 0.0 0.15608850640173808 0.0 46-47 0.0 0.0 0.0 0.23202345546204306 0.0 48-49 0.0 0.0 0.0 0.29973211837414837 0.0 50-51 0.0 0.0 0.0 0.3760889282625662 0.0 52-53 0.0 0.0 0.0 0.4754371532831319 0.0 54-55 0.0 0.0 0.0 0.5646607184289902 0.0 56-57 0.0 0.0 0.0 0.6564154485435255 0.0 58-59 0.0 0.0 0.0 0.797527895547259 0.0 60-61 0.0 0.0 0.0 1.0042397013225337 0.0 62-63 0.0 0.0 0.0 1.1928114914889578 0.0 64-65 0.0 0.0 0.0 1.3164167141260097 0.0 66-67 0.0 0.0 0.0 1.4857938366133012 0.0 68-69 0.0 0.0 0.0 1.6868105212090532 0.0 70-71 0.0 0.0 0.0 1.8646248602586009 0.0 72-73 0.0 0.0 0.0 2.1683646564998207 0.0 74-75 0.0 0.0 0.0 2.3987006686494126 0.0 76-77 0.0 0.0 0.0 2.586217806745555 0.0 78-79 0.0 0.0 0.0 2.79757008163007 0.0 80-81 0.0 0.0 0.0 3.02685144170938 0.0 82-83 0.0 0.0 0.0 3.3090763357168473 0.0 84-85 0.0 0.0 0.0 3.8220591027020188 0.0 86-87 0.0 0.0 0.0 4.454006623215001 0.0 88-89 0.0 0.0 0.0 4.938724714716615 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAACGT 25 0.0050145816 57.005085 8 CGTCACT 40 5.3658546E-4 47.524357 1 ATATGGG 55 0.0025770448 34.54122 3 GTCACTA 55 0.0025770448 34.54122 2 TATATGG 70 2.0498908E-4 33.92441 2 GTATATG 75 3.0618784E-4 31.682903 1 CATGGGT 165 7.6397555E-11 31.66949 4 GTACCAA 30 0.0039468943 31.66949 88-89 TCCGCGT 30 0.0039468943 31.66949 84-85 AAGTACT 75 3.0694963E-4 31.669489 4 GTTATGT 60 0.003947023 31.662786 3 TACCTGG 125 2.14397E-7 30.396275 2 ATGGGCA 110 2.7194528E-6 30.229967 5 GTTCTGA 65 0.0058201985 29.245756 1 AGTACTC 85 6.3886715E-4 27.943668 5 CACTACC 85 6.3965877E-4 27.937754 2 TCAGACC 85 6.3965877E-4 27.937754 3 GGGCAAC 70 0.008374675 27.145277 7 ATACCAT 45 8.9461176E-4 26.385654 94-95 TGGGAGT 145 7.837025E-7 26.20923 6 >>END_MODULE