##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 237045 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04545550422915 31.0 31.0 34.0 31.0 34.0 2 32.15598304119471 33.0 31.0 34.0 30.0 34.0 3 32.1633613870784 33.0 31.0 34.0 30.0 34.0 4 35.81138180514248 37.0 35.0 37.0 35.0 37.0 5 35.77206015735409 37.0 35.0 37.0 35.0 37.0 6 35.94997574300238 37.0 35.0 37.0 35.0 37.0 7 35.898411693982155 37.0 35.0 37.0 35.0 37.0 8 35.93778818367821 37.0 35.0 37.0 35.0 37.0 9 37.490227593916764 39.0 37.0 39.0 35.0 39.0 10-11 37.42464300027421 39.0 37.0 39.0 34.5 39.0 12-13 37.27618806555718 39.0 37.0 39.0 34.0 39.0 14-15 38.01764432913582 40.0 37.0 41.0 33.0 41.0 16-17 38.079862473370035 39.5 37.5 41.0 33.5 41.0 18-19 38.35267143369403 40.0 37.5 41.0 34.0 41.0 20-21 38.421164757746425 40.0 38.0 41.0 34.0 41.0 22-23 38.36395832015018 40.0 37.5 41.0 34.0 41.0 24-25 38.25191419350756 40.0 37.0 41.0 34.0 41.0 26-27 37.80750490413213 40.0 36.5 41.0 33.0 41.0 28-29 37.556402792718686 39.0 36.0 41.0 33.0 41.0 30-31 37.220901094728845 39.0 35.0 41.0 33.0 41.0 32-33 36.73692336898057 38.0 35.0 41.0 33.0 41.0 34-35 36.44382290282436 38.0 35.0 40.5 33.0 41.0 36-37 36.02394271129954 38.0 35.0 40.0 31.0 41.0 38-39 35.633084435444744 37.5 35.0 40.0 30.5 41.0 40-41 35.206730789512534 37.0 35.0 40.0 30.0 41.0 42-43 34.80535552321289 37.0 35.0 40.0 28.0 41.0 44-45 34.1652175747221 37.0 34.5 40.0 23.5 41.0 46-47 33.25024362462824 36.0 33.0 40.0 16.0 41.0 48-49 32.48743065662638 36.0 33.0 40.0 2.0 41.0 50-51 32.0735219051235 36.0 33.0 40.0 2.0 41.0 52-53 31.591689341686177 35.0 32.5 40.0 2.0 41.0 54-55 31.371564470881054 35.0 32.0 40.0 2.0 41.0 56-57 31.182897762028304 35.0 32.0 40.0 2.0 41.0 58-59 30.985285494315427 35.0 31.0 39.0 2.0 41.0 60-61 30.546373051530303 35.0 31.0 39.0 2.0 40.5 62-63 29.958573266679323 35.0 30.0 38.5 2.0 40.0 64-65 29.378596890885696 35.0 28.5 38.0 2.0 40.0 66-67 28.92588116180472 35.0 28.0 37.0 2.0 39.5 68-69 28.515250268936278 35.0 27.5 37.0 2.0 39.0 70-71 28.068605117171845 34.0 26.0 36.0 2.0 39.0 72-73 27.52577358729355 34.0 26.0 35.0 2.0 38.0 74-75 27.017931194498935 33.5 24.5 35.0 2.0 37.0 76-77 25.68078002067118 31.5 22.5 34.5 2.0 36.0 78-79 26.087375814718726 33.0 22.5 35.0 2.0 36.0 80-81 25.940667383830075 33.0 20.5 35.0 2.0 36.0 82-83 25.578664388618193 33.0 19.0 35.0 2.0 35.5 84-85 25.17950389166614 33.0 14.5 35.0 2.0 35.0 86-87 24.77045708620726 33.0 4.5 35.0 2.0 35.0 88-89 24.185057689468245 32.5 2.0 35.0 2.0 35.0 90-91 23.48710371448459 31.5 2.0 35.0 2.0 35.0 92-93 23.016212111624377 31.0 2.0 34.0 2.0 35.0 94-95 22.543479086249448 31.0 2.0 34.0 2.0 35.0 96-97 21.910485350882745 31.0 2.0 34.0 2.0 35.0 98-99 21.103655424075598 30.0 2.0 34.0 2.0 35.0 100-101 19.218275011073846 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19023704352242277 1101 2 0.24156310634794664 1101 3 0.2605994727317835 1101 4 0.15613349854123726 1101 5 0.16038766928388526 1101 6 0.209650663658266 1101 7 0.2700700015665447 1101 8 0.0898057559458536 1101 9 0.3211752651176809 1101 10-11 0.33141643679525146 1101 12-13 0.25918516387547896 1101 14-15 0.21677407231666024 1101 16-17 0.045860684785189676 1101 18-19 0.4493754663410314 1101 20-21 0.5942410679253882 1101 22-23 0.7238979338941434 1101 24-25 0.49161602007752947 1101 26-27 0.4066529981235121 1101 28-29 0.39948217780482764 1101 30-31 0.35936713772698425 1101 32-33 0.30662997660225955 1101 34-35 0.10869574540003413 1101 36-37 0.16644574212823926 1101 38-39 0.44136940074521647 1101 40-41 0.27497388908800957 1101 42-43 -0.23211985816468683 1101 44-45 -0.14797805830286848 1101 46-47 -0.23641737889874292 1101 48-49 -0.23919989635023597 1101 50-51 -0.681557227449467 1101 52-53 -0.9284281038716422 1101 54-55 -0.600635418958614 1101 56-57 -0.8210825709680165 1101 58-59 -0.5849708795079565 1101 60-61 -0.41404577229267403 1101 62-63 -0.41582303022749656 1101 64-65 -0.23892133812548622 1101 66-67 -0.33614160115422465 1101 68-69 -0.17203018763668254 1101 70-71 -0.2899065548553601 1101 72-73 -0.16789839916140537 1101 74-75 -0.38662316663731744 1101 76-77 -0.23803184469545968 1101 78-79 0.03328912920104443 1101 80-81 0.07808292316972043 1101 82-83 -0.09622035676761698 1101 84-85 -0.2964576426068213 1101 86-87 -0.24103997412048628 1101 88-89 0.35831591667663787 1101 90-91 0.11550617770132732 1101 92-93 0.352487150745997 1101 94-95 0.35531292676968107 1101 96-97 0.26723461429500617 1101 98-99 0.36927639129537937 1101 100-101 0.4328171194748478 1102 1 0.25740934472641896 1102 2 0.32262902023136064 1102 3 0.33736295820896345 1102 4 0.394297569012636 1102 5 0.26791436814791325 1102 6 0.24869562658595612 1102 7 0.1205903743303054 1102 8 0.20041047671314516 1102 9 0.21701568413679695 1102 10-11 0.31360302274178764 1102 12-13 0.12143092946965339 1102 14-15 0.4106894522227904 1102 16-17 0.35998047255473153 1102 18-19 0.4579973287993724 1102 20-21 0.39342139579424185 1102 22-23 0.3253876284727468 1102 24-25 0.474542351619597 1102 26-27 0.45146287500262616 1102 28-29 0.8244022512692197 1102 30-31 0.698689292682424 1102 32-33 0.7486152157016406 1102 34-35 0.7759122612833025 1102 36-37 1.3053903717452684 1102 38-39 0.926183358979344 1102 40-41 1.0684403199430577 1102 42-43 0.96785803449567 1102 44-45 1.0716668638112736 1102 46-47 1.0937047216386375 1102 48-49 0.9052290540613015 1102 50-51 0.807142520866968 1102 52-53 0.9445312186234212 1102 54-55 1.0494819199979197 1102 56-57 0.676502627317813 1102 58-59 1.072322842007587 1102 60-61 0.7914419496310039 1102 62-63 0.5863281839756205 1102 64-65 0.4273513644117557 1102 66-67 0.6671915054371631 1102 68-69 0.40011626498558783 1102 70-71 0.07227118815531952 1102 72-73 0.1427692424956284 1102 74-75 0.4840999134252648 1102 76-77 0.6508702708068839 1102 78-79 0.6991196849455541 1102 80-81 0.8581233966069028 1102 82-83 0.7987017573742818 1102 84-85 0.7310149783033211 1102 86-87 1.2399477137919028 1102 88-89 1.1729118625306612 1102 90-91 0.9764425085631743 1102 92-93 1.1625680976203618 1102 94-95 1.1259237695694928 1102 96-97 1.270134076916598 1102 98-99 1.5466282721198112 1102 100-101 1.785539723379344 1103 1 0.16873572326591102 1103 2 0.244015011289477 1103 3 0.304305106969295 1103 4 0.15215558283190234 1103 5 0.09872997578915488 1103 6 0.13875117461468278 1103 7 -0.04876337177456236 1103 8 0.07325968413761075 1103 9 0.19801419382383045 1103 10-11 0.08231661168638027 1103 12-13 0.30035316222944175 1103 14-15 0.39145086666528073 1103 16-17 0.5557025969281213 1103 18-19 0.2973144944950761 1103 20-21 0.1727534646196034 1103 22-23 0.27935728028144524 1103 24-25 0.4039218394938686 1103 26-27 0.005046400270209972 1103 28-29 0.009234929768069833 1103 30-31 0.17106769669272381 1103 32-33 0.2774900294125189 1103 34-35 0.6104875220880714 1103 36-37 0.39909379946509205 1103 38-39 0.8853013646696368 1103 40-41 0.5726402928464509 1103 42-43 0.46594433716469297 1103 44-45 0.9451257447217216 1103 46-47 0.7677834372598298 1103 48-49 1.2907459216944162 1103 50-51 1.2381986108276877 1103 52-53 1.1638612552038374 1103 54-55 1.0655197197540467 1103 56-57 0.912954327629425 1103 58-59 0.760593401552363 1103 60-61 1.1949611204750639 1103 62-63 1.2164426671079873 1103 64-65 1.4030491018457063 1103 66-67 1.7069896357718086 1103 68-69 1.55400801407108 1103 70-71 1.5645994634567693 1103 72-73 1.2980835630483973 1103 74-75 1.3819118630255893 1103 76-77 1.3492851197433424 1103 78-79 1.256378186503607 1103 80-81 0.9793637434433755 1103 82-83 1.1914669653406804 1103 84-85 1.3119491335668378 1103 86-87 1.7883828851778958 1103 88-89 2.1417338378727564 1103 90-91 2.6796500685143982 1103 92-93 2.6013297830288415 1103 94-95 2.6866201521734716 1103 96-97 2.7812944887849085 1103 98-99 3.0803974090931234 1103 100-101 3.207010391310522 1104 1 -0.05389878508288248 1104 2 0.06757716342305997 1104 3 0.2047098969844754 1104 4 0.03837585606516569 1104 5 -0.02608027889122866 1104 6 0.08054574368198075 1104 7 0.006271204039236977 1104 8 0.053003317128002436 1104 9 0.12844090996483715 1104 10-11 -0.00992491302268661 1104 12-13 0.2014326022699251 1104 14-15 0.04161952917160505 1104 16-17 0.1827054641907253 1104 18-19 0.23225293268026093 1104 20-21 0.1819961699662045 1104 22-23 0.2919985351304959 1104 24-25 -0.016089966881580153 1104 26-27 0.056631827829669135 1104 28-29 0.36168896923509664 1104 30-31 -0.3193016741961259 1104 32-33 -0.012584182090265017 1104 34-35 0.08720366308993022 1104 36-37 -0.46945907623064187 1104 38-39 -0.5291361458717745 1104 40-41 -0.5142461972867878 1104 42-43 -0.35772744559800174 1104 44-45 -0.2107352773730753 1104 46-47 -0.20145252158652482 1104 48-49 -0.20251601117791296 1104 50-51 -0.3088076296850524 1104 52-53 -0.0107297382183269 1104 54-55 0.11094896583264813 1104 56-57 -0.23109188589730678 1104 58-59 0.15791166770179998 1104 60-61 -0.013969559234837448 1104 62-63 -0.03405488728567363 1104 64-65 0.03221084918927275 1104 66-67 -4.230813811680889E-4 1104 68-69 0.4394187072739797 1104 70-71 0.5454478358635804 1104 72-73 0.5589201290797696 1104 74-75 0.8498744199491846 1104 76-77 1.1069592637811247 1104 78-79 0.7085960984617792 1104 80-81 0.31661285796521454 1104 82-83 0.33431567507251003 1104 84-85 0.3747168852956264 1104 86-87 -0.32683047289260614 1104 88-89 -0.0035267456995136115 1104 90-91 0.05261346875052553 1104 92-93 0.09689395911250287 1104 94-95 -0.039767096941044855 1104 96-97 0.2615609755449029 1104 98-99 1.1319912697778918 1104 100-101 1.1079970669725192 1105 1 0.3285842081143997 1105 2 0.3349241021985705 1105 3 0.08736295820896345 1105 4 0.18514456354815678 1105 5 -0.07327415644225255 1105 6 0.12697431511055157 1105 7 0.29572698635215744 1105 8 0.3523230450191548 1105 9 0.5620293453389849 1105 10-11 0.46592542711337614 1105 12-13 0.4364240988685637 1105 14-15 0.4031416380151427 1105 16-17 0.4463534233743971 1105 18-19 0.4547869462857079 1105 20-21 0.4004910679253939 1105 22-23 0.3450597596202911 1105 24-25 0.2883743188327088 1105 26-27 0.12202298429225067 1105 28-29 0.3388148195752265 1105 30-31 0.276319074103192 1105 32-33 0.0880960900185741 1105 34-35 0.04647237057292131 1105 36-37 0.4214422843135779 1105 38-39 0.15623800378808994 1105 40-41 0.8956602652982468 1105 42-43 0.8869154115448552 1105 44-45 0.5914756069806657 1105 46-47 0.8655967981413681 1105 48-49 0.8951880704547435 1105 50-51 0.8181056356210732 1105 52-53 1.03684679239392 1105 54-55 1.5755748161727858 1105 56-57 1.213763556279563 1105 58-59 1.0832518037562266 1105 60-61 0.25312227749985894 1105 62-63 0.19455905829255826 1105 64-65 1.220008468236891 1105 66-67 1.0703677349453606 1105 68-69 1.1500479589746568 1105 70-71 0.8088832100132421 1105 72-73 0.7559864556103797 1105 74-75 0.7509373451192545 1105 76-77 0.6010068828287451 1105 78-79 -0.1083256702457085 1105 80-81 0.1463747627271239 1105 82-83 -0.08630507322681069 1105 84-85 0.3338155247514081 1105 86-87 0.7751253094203214 1105 88-89 0.7642028461372199 1105 90-91 0.6799944211314752 1105 92-93 0.32240416319413256 1105 94-95 0.6337448078208574 1105 96-97 1.2593075741843585 1105 98-99 1.6647293650159867 1105 100-101 0.9480055703738799 1106 1 -0.231687109715061 1106 2 -0.1046107815223607 1106 3 -0.18124169295382586 1106 4 -0.2762895449789724 1106 5 -0.17007648202364578 1106 6 -0.31081638256387123 1106 7 -0.22714123070210235 1106 8 -0.06318083094983962 1106 9 -0.1582225926455152 1106 10-11 -0.10190403025097083 1106 12-13 -0.538624350743838 1106 14-15 -0.04167425345772102 1106 16-17 -0.21425704174188098 1106 18-19 -0.2935657668925913 1106 20-21 -0.045293815795538706 1106 22-23 -0.18982396130994772 1106 24-25 -0.6140966113998445 1106 26-27 -0.4937715893511623 1106 28-29 -0.686863474998411 1106 30-31 -0.9602603832611507 1106 32-33 -1.2382604991287067 1106 34-35 -1.7018803426053708 1106 36-37 -0.8583057777019221 1106 38-39 -0.7131418411731474 1106 40-41 -0.9246401222986478 1106 42-43 -1.2866311000830493 1106 44-45 -1.586625168213132 1106 46-47 -1.9919128917811122 1106 48-49 -2.17603285977782 1106 50-51 -1.8820397132161375 1106 52-53 -1.687426463420035 1106 54-55 -1.7608592923543505 1106 56-57 -1.691129854573152 1106 58-59 -1.8305078861662594 1106 60-61 -1.6134474899420006 1106 62-63 -1.3540359029477536 1106 64-65 -1.8378888185848155 1106 66-67 -1.5100101720313823 1106 68-69 -1.3870644441261142 1106 70-71 -1.3041012861107895 1106 72-73 -0.9979379629942713 1106 74-75 -0.9523087789117533 1106 76-77 -0.7770938923650554 1106 78-79 -0.8466008640441558 1106 80-81 -0.8142841520015587 1106 82-83 -0.45960933679270255 1106 84-85 -0.38992478532610875 1106 86-87 -0.39280104716882747 1106 88-89 -0.708035525955804 1106 90-91 -0.9500443385584454 1106 92-93 -0.1880609530849391 1106 94-95 -0.25205945574503374 1106 96-97 -0.6488804103117687 1106 98-99 -0.8231582318832373 1106 100-101 -0.9007831893160407 1107 1 0.09996101970860138 1107 2 0.32622845002465795 1107 3 0.21562382777418065 1107 4 -0.04565253790111967 1107 5 0.20444323486209726 1107 6 0.22588735858880682 1107 7 0.2689153921492604 1107 8 0.18130855226553422 1107 9 -0.0055431184291308 1107 10-11 0.07969354305539866 1107 12-13 0.14357989597001364 1107 14-15 0.07116700033397905 1107 16-17 0.2623860320700473 1107 18-19 0.14192462744512824 1107 20-21 0.07032802444712871 1107 22-23 0.09071193353333484 1107 24-25 0.023972144919667926 1107 26-27 0.3439432741473212 1107 28-29 0.07051771812595575 1107 30-31 0.238728494393051 1107 32-33 0.3765018871200283 1107 34-35 0.046653529993214704 1107 36-37 0.2781451828643 1107 38-39 0.16203510523736497 1107 40-41 -0.002799879629286295 1107 42-43 -0.5266715449768853 1107 44-45 -0.4125099002657038 1107 46-47 -0.32851552069921297 1107 48-49 -0.8662249730235203 1107 50-51 -1.0310791469876222 1107 52-53 -0.8596205989104284 1107 54-55 -0.6410918504938827 1107 56-57 -0.7319791973436267 1107 58-59 -0.6171105150843559 1107 60-61 -0.8995951138044909 1107 62-63 -1.2162199272146879 1107 64-65 -0.8016400824877437 1107 66-67 -0.8070779172285505 1107 68-69 -0.1990462439238918 1107 70-71 -0.7233631667983538 1107 72-73 -0.769828761780925 1107 74-75 -0.6076677273445164 1107 76-77 -0.48993514615676403 1107 78-79 -0.5574198731442586 1107 80-81 -0.3880455271279537 1107 82-83 -0.46284492829927615 1107 84-85 -0.2576699824949671 1107 86-87 0.2858137152174187 1107 88-89 0.1541484983111303 1107 90-91 0.5709364501169851 1107 92-93 0.6680563371071777 1107 94-95 0.6990527788353482 1107 96-97 0.6197242408510242 1107 98-99 1.3323018287841037 1107 100-101 1.5658497732724292 1108 1 -0.021450659389088855 1108 2 0.010656319771790379 1108 3 0.22648429712109674 1108 4 0.08887538642123616 1108 5 0.03002082263724759 1108 6 -0.12375790246267115 1108 7 0.07892084967154744 1108 8 0.018710771657929115 1108 9 0.20238848062489723 1108 10-11 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0.4744066163850391 2316 66-67 0.23819180901943682 2316 68-69 0.9713442552709566 2316 70-71 0.3524017285317598 2316 72-73 0.4839957148696392 2316 74-75 0.41829845623036377 2316 76-77 0.6218402161620737 2316 78-79 0.8032484038283698 2316 80-81 0.8500784664308263 2316 82-83 0.6375375193657788 2316 84-85 0.22501922845511402 2316 86-87 0.24734753164254286 2316 88-89 0.3459158091001804 2316 90-91 0.4114759026129562 2316 92-93 0.6349041631941326 2316 94-95 1.0432355485616007 2316 96-97 1.0100020186288035 2316 98-99 1.0300071427937638 2316 100-101 1.1209222370405492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 8.0 6 28.0 7 101.0 8 158.0 9 326.0 10 498.0 11 912.0 12 1482.0 13 2126.0 14 2459.0 15 3492.0 16 4926.0 17 7359.0 18 6212.0 19 3017.0 20 2461.0 21 2491.0 22 2746.0 23 3135.0 24 3266.0 25 3490.0 26 3847.0 27 4365.0 28 5354.0 29 6000.0 30 7041.0 31 8488.0 32 10196.0 33 12634.0 34 17702.0 35 28797.0 36 29338.0 37 31365.0 38 19843.0 39 1369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.76278282595942 18.565537965467904 14.378806359442725 13.292872849129944 2 14.576135332953658 26.39076968508089 38.72429285578687 20.308802126178573 3 23.97392900082263 34.6862410090911 29.56063194752051 11.779198042565756 4 10.884431226138497 18.360226961125527 36.295218207513344 34.460123605222634 5 10.039110128552926 46.10480839412209 28.565582243073457 15.290499234251529 6 22.99689932291337 37.37729123162269 18.686325381256726 20.939484064207218 7 23.182518087283004 43.0078677044443 17.64348541416187 16.166128794110822 8 27.499841802189458 29.987976966398783 19.135185302368747 23.37699592904301 9 31.310088801704318 19.17104347275834 20.206711805775274 29.312155919762073 10-11 24.276614144993566 29.913096669408763 22.147693475922292 23.662595709675376 12-13 32.55904216272771 28.871807420032567 23.970822751166498 14.59832766607322 14-15 27.89660191102955 27.513341348689067 30.512349975743003 14.077706764538378 16-17 16.657385728448183 32.22932354616212 31.95152819084984 19.161762534539857 18-19 16.277710983146658 30.288341876015103 38.61334345799321 14.820603682845029 20-21 19.285830297686722 27.947916952302204 36.65134605527654 16.114906694734533 22-23 17.52432058453776 28.752499557040522 35.431273782705176 18.29190607571654 24-25 17.86369676643675 29.343373621042417 37.17585268619882 15.617076926322007 26-27 19.75004745934316 27.00183509460229 38.523908962433296 14.724208483621254 28-29 16.75002056601903 27.882037533512065 40.69646032445796 14.671481576010953 30-31 15.82378873209728 32.8777236389715 38.068721128899575 13.229766500031639 32-33 14.822080195743425 32.53538357695796 36.851441709380076 15.79109451791854 34-35 15.706300491467864 29.97953974983653 37.15792360100403 17.156236157691577 36-37 18.02699493133146 30.502922447051077 37.325692011415576 14.144390610201881 38-39 20.005695121179524 28.181147039591636 37.891961441920316 13.921196397308528 40-41 15.829306311684094 31.019070258959815 39.062918565923276 14.088704863432817 42-43 14.994598973805024 33.555985012152306 36.56811200378072 14.88130401026195 44-45 15.844038051846695 33.97498365289291 33.66428315298783 16.516695142272564 46-47 15.551688497964522 33.014828408108166 31.65875677613955 19.77472631778776 48-49 17.280896032398914 32.56639034782425 31.93359910565504 18.21911451412179 50-51 19.249087725959203 34.077284903710265 31.109283047522624 15.564344322807905 52-53 16.22729861418718 34.60777489506212 32.11035879263431 17.05456769811639 54-55 14.033200447172478 34.09057351979582 32.75770423337341 19.118521799658293 56-57 17.17543082537071 35.98472863802232 28.237676390558754 18.60216414604822 58-59 16.517960724756904 33.75793625682888 29.872176169081822 19.851926849332404 60-61 17.126916830137738 32.42612162247674 31.000864814697632 19.446096732687888 62-63 21.24427851251872 31.982745892130186 30.034170727077136 16.738804868273956 64-65 19.649644582252314 32.765719589107555 31.812314117572615 15.77232171106752 66-67 17.184922694003248 33.09793499124638 32.40566137231327 17.31148094243709 68-69 17.280052310742686 35.29519711447194 30.907211710856586 16.51753886392879 70-71 17.164040583011662 35.30236874854985 28.5587124807526 18.97487818768588 72-73 16.89974477419899 33.374253833660276 28.31761901748613 21.408382374654604 74-75 17.452593389440825 34.11061190913118 29.570334746567106 18.866459954860893 76-77 18.30622877512709 31.95912168575587 31.867366955641334 17.86728258347571 78-79 16.212322554789175 31.31873694868063 34.06230884431226 18.406631652217932 80-81 15.12898394819549 35.45044189921745 31.7673859393786 17.653188213208463 82-83 14.915975692327812 34.59118435568005 29.910417664193854 20.582422287798284 84-85 16.085376374478212 31.885152365905977 29.687463746258615 22.342007513357196 86-87 17.331730262186504 31.5878841570166 30.737834588369296 20.3425509924276 88-89 18.603640658946613 29.03541521652007 32.19958235778017 20.161361766753146 90-91 15.90441151652209 30.594348060890404 33.22201876666554 20.279221655921965 92-93 14.25536941490147 33.125208300813796 32.10595814155596 20.513464142728772 94-95 14.164610095129618 33.44702482650973 30.312177012803478 22.076188065557172 96-97 14.461389187706974 31.460271256512478 28.96201143242844 25.116328123352105 98-99 16.62539444153828 30.362084676262636 29.3671217157996 23.645399166399486 100-101 17.840386481583057 30.665161807518672 30.40504620058225 21.08940551031602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3169.0 1 2074.5 2 1272.5 3 1584.5 4 1326.0 5 1275.0 6 2399.5 7 4525.0 8 5873.5 9 5625.0 10 4396.0 11 3312.0 12 3057.5 13 2846.0 14 2480.0 15 2079.5 16 1709.0 17 1471.0 18 1373.5 19 1317.0 20 1233.5 21 1219.5 22 1267.5 23 1332.5 24 1433.5 25 1530.0 26 1634.0 27 1796.5 28 1938.5 29 2117.0 30 2414.0 31 2584.0 32 2800.5 33 3249.0 34 3663.5 35 3856.0 36 4177.0 37 4533.5 38 4575.5 39 4602.0 40 4563.0 41 4469.5 42 4359.5 43 4405.0 44 5036.5 45 5120.0 46 5322.0 47 7844.0 48 23555.5 49 23484.5 50 10254.0 51 7786.5 52 3416.5 53 1943.0 54 1613.5 55 1524.0 56 1797.5 57 2052.5 58 2041.0 59 2223.5 60 2450.5 61 2559.0 62 2156.5 63 1082.5 64 409.5 65 325.0 66 299.0 67 233.0 68 176.0 69 155.5 70 146.5 71 120.5 72 102.5 73 123.0 74 114.5 75 69.5 76 43.5 77 33.5 78 32.5 79 25.0 80 16.0 81 12.0 82 5.5 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04471724777995739 2 0.0 3 0.0 4 0.0 5 0.009280938218481723 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046404691092408615 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.1093041405640279E-4 22-23 0.0012655824843384167 24-25 0.0 26-27 0.0 28-29 0.0014765128983948196 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.1093041405640279E-4 38-39 0.0 40-41 2.1093041405640279E-4 42-43 0.022358623889978696 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1093041405640279E-4 84-85 2.1093041405640279E-4 86-87 0.0 88-89 0.0 90-91 0.012233964015271362 92-93 0.0033748866249024446 94-95 0.0 96-97 0.0 98-99 4.2186082811280557E-4 100-101 0.014765128983948194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 237045.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.012297243139486 #Duplication Level Percentage of deduplicated Percentage of total 1 76.48554706468678 25.249636145035755 2 11.784701101541135 7.780801113712586 3 4.355048943185013 4.313105106625324 4 2.136631993252741 2.8214052184184437 5 1.2715004983770797 2.0987576198612077 6 0.8101822271066015 1.6047585901411126 7 0.5392695581056559 1.2461768862452278 8 0.3859227643315358 1.0192157607205383 9 0.311805147340711 0.926406378535721 >10 1.5807498658215555 9.754266067624291 >50 0.14951312392976718 3.4440718007129445 >100 0.14567945408541416 10.18878272058048 >500 0.014056789429294348 3.301482840810817 >1k 0.024279909014235695 15.608006918517582 >5k 0.005111559792470672 10.643126832457972 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7297 3.0783184627391424 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6405 2.70201860406252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6024 2.5412896285515405 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5503 2.321500137104769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 2.0084794026450674 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4513 1.9038579172730916 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3252 1.3718914130228437 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2078 0.8766268008184099 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1959 0.8264253622729861 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1918 0.8091290683203611 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1894 0.7990044084456537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1871 0.7893016093990592 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 0.7403657533379738 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1613 0.6804615157459554 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1539 0.6492438144656077 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1510 0.6370098504503364 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1452 0.6125419224197937 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1310 0.5526376848277753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1192 0.5028581071104643 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1168 0.4927334472357569 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1099 0.4636250500959733 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1084 0.45729713767428126 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1030 0.4345166529561898 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 935 0.3944398742854733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 935 0.3944398742854733 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 922 0.38895568352000676 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 843 0.35562867809909515 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 728 0.3071146828661225 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.3020523529287688 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 606 0.2556476618363602 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 568 0.23961695036807357 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 562 0.23708578539939673 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 507 0.21388343985319244 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 504 0.212617857368854 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 493 0.20797738825961315 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 489 0.2062899449471619 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 487 0.20544622329093631 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 473 0.19954017169735702 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 454 0.19152481596321375 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 450 0.18983737265076253 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 441 0.18604062519774728 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 415 0.17507224366681431 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 401 0.16916619207323502 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 373 0.15735408888607647 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 360 0.15186989812061 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 356 0.15018245480815878 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 356 0.15018245480815878 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 355 0.149760593980046 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 346 0.14596384652703073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 341 0.1438545423864667 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 335 0.14132337741778989 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 329 0.13879221244911302 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 318 0.13415174333987218 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 309 0.13035499588685692 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 308 0.12993313505874413 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 306 0.1290894134025185 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 295 0.12444894429327764 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 289 0.12191777932460081 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.1206521968402624 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 285 0.12023033601214958 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 267 0.11263684110611909 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 264 0.11137125862178067 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.10884009365310385 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 256 0.10799637199687823 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 247 0.10419962454386299 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 246 0.10377776371575018 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 244 0.10293404205952456 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 238 0.10040287709084773 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.2186082811280557E-4 0.0 18-19 0.0 0.0 0.0 4.2186082811280557E-4 0.0 20-21 0.0 0.0 0.0 0.0010546520702820138 0.0 22-23 0.0 0.0 0.0 0.0021093041405640276 0.0 24-25 0.0 0.0 0.0 0.004218608281128056 0.0 26-27 0.0 0.0 0.0 0.008648146976312516 0.0 28-29 0.0 0.0 0.0 0.01434326815583539 0.0 30-31 0.0 0.0 0.0 0.024046067202429917 0.0 32-33 0.0 0.0 0.0 0.03438165749119365 0.0 34-35 0.0 0.0 0.0 0.04134236115505495 0.0 36-37 0.0 0.0 0.0 0.0529435339281571 0.0 38-39 0.0 0.0 0.0 0.06391191545909004 0.0 40-41 0.0 0.0 0.0 0.08901263473180199 0.0 42-43 0.0 0.0 0.0 0.11601172773102153 0.0 44-45 0.0 0.0 0.0 0.16009618426880973 0.0 46-47 0.0 0.0 0.0 0.23814043746967872 0.0 48-49 0.0 0.0 0.0 0.30521630913961484 0.0 50-51 0.0 0.0 0.0 0.3817840494420891 0.0 52-53 0.0 0.0 0.0 0.4819759961188804 0.0 54-55 0.0 0.0 0.0 0.5747853783036976 0.0 56-57 0.0 0.0 0.0 0.669282203800966 0.0 58-59 0.0 0.0 0.0 0.8125039549452635 0.0 60-61 0.0 0.0 0.0 1.0225906473454407 0.0 62-63 0.0 0.0 0.0 1.214326393722711 0.0 64-65 0.0 0.0 0.0 1.3387753380159886 0.0 66-67 0.0 0.0 0.0 1.5140585120968593 0.0 68-69 0.0 0.0 0.0 1.7190828745596827 0.0 70-71 0.0 0.0 0.0 1.8964753527811176 0.0 72-73 0.0 0.0 0.0 2.204011896475353 0.0 74-75 0.0 0.0 0.0 2.4373009344217342 0.0 76-77 0.0 0.0 0.0 2.6290366807990044 0.0 78-79 0.0 0.0 0.0 2.8454512856208733 0.0 80-81 0.0 0.0 0.0 3.0810605581218757 0.0 82-83 0.0 0.0 0.0 3.370246155793204 0.0 84-85 0.0 0.0 0.0 3.8963066084498728 0.0 86-87 0.0 0.0 0.0 4.53732413676728 0.0 88-89 0.0 0.0 0.0 5.029635723174925 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTAGA 20 0.0020698304 71.26915 2 AGATCCT 20 0.0020732994 71.23909 6 GGGATGC 40 7.515082E-6 59.39096 7 ATATAGT 25 0.005011036 57.015324 9 GATTGGC 50 4.2196734E-7 57.015324 9 GGGATCG 25 0.005011036 57.015324 7 GATATAG 35 2.7652594E-4 54.357674 1 GGCTTAC 35 2.779723E-4 54.30031 7 GCAGTTC 45 1.5098809E-5 52.79197 8 AGATTGG 60 1.4844809E-6 47.512768 8 GCCAAAT 20 5.389026E-4 47.492725 18-19 CCATATA 55 4.9434897E-5 43.193424 2 AGGCTTA 55 4.9558228E-5 43.1752 6 ATACGTA 25 0.0016160744 37.99418 24-25 GGATTGG 80 1.072027E-5 35.634575 9 GGATTTA 95 8.643019E-7 35.00941 8 TGGGGAA 190 0.0 34.99464 6 ATGGGTG 125 5.5970304E-9 34.20919 5 GATATGA 85 1.6237718E-5 33.53842 2 ATGGGCA 115 1.0280564E-7 33.05236 5 >>END_MODULE