##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 203705 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90782258658354 33.0 31.0 34.0 31.0 34.0 2 31.84540880194399 33.0 31.0 34.0 30.0 34.0 3 31.784963550231954 33.0 31.0 34.0 30.0 34.0 4 35.47241353918657 37.0 35.0 37.0 33.0 37.0 5 35.48279620038782 37.0 35.0 37.0 33.0 37.0 6 35.68966397486562 37.0 35.0 37.0 35.0 37.0 7 35.65637073218625 37.0 35.0 37.0 35.0 37.0 8 35.7103016617167 37.0 35.0 37.0 35.0 37.0 9 37.167703296433565 39.0 37.0 39.0 34.0 39.0 10-11 37.14232345794163 39.0 37.0 39.0 34.0 39.0 12-13 36.98051103311161 39.0 37.0 39.0 33.0 39.0 14-15 37.638796298569005 40.0 37.0 41.0 32.5 41.0 16-17 37.71210083208561 39.5 37.0 41.0 32.5 41.0 18-19 38.038140939103116 40.0 38.0 41.0 33.5 41.0 20-21 38.25066640485015 40.0 38.0 41.0 34.0 41.0 22-23 38.238901843351904 40.0 38.0 41.0 34.0 41.0 24-25 38.073684985641 40.0 37.5 41.0 34.0 41.0 26-27 37.768871652634935 40.0 36.5 41.0 33.5 41.0 28-29 37.51136692766501 39.5 36.0 41.0 33.0 41.0 30-31 36.893223043126085 38.5 35.0 40.0 33.0 41.0 32-33 36.30024299845364 38.0 35.0 40.0 33.0 41.0 34-35 36.0258707444589 38.0 35.0 40.0 31.5 41.0 36-37 35.606281141847276 37.5 35.0 40.0 31.0 41.0 38-39 35.19114896541568 37.0 35.0 40.0 30.0 41.0 40-41 34.645536437495394 36.0 35.0 40.0 28.0 41.0 42-43 34.10983039198841 36.0 35.0 40.0 24.0 41.0 44-45 33.428926634103235 35.5 33.5 40.0 19.5 41.0 46-47 32.3184457917086 35.0 33.0 40.0 3.5 41.0 48-49 31.460150708131856 35.0 32.5 40.0 2.0 41.0 50-51 30.37072973171989 35.0 30.0 39.0 2.0 40.5 52-53 30.3913551459218 35.0 31.0 39.0 2.0 40.0 54-55 30.594347217790432 35.0 31.0 39.0 2.0 40.5 56-57 30.400564541862007 35.0 31.0 39.0 2.0 41.0 58-59 30.178530227534917 35.0 31.0 39.0 2.0 41.0 60-61 29.732922608674308 35.0 29.5 39.0 2.0 40.5 62-63 29.206089688520166 35.0 28.0 38.0 2.0 40.0 64-65 28.60450651677671 35.0 26.5 38.0 2.0 40.0 66-67 28.05263493777767 35.0 26.0 37.0 2.0 39.5 68-69 27.65344738715299 35.0 25.5 36.0 2.0 39.0 70-71 27.082241476645148 34.0 23.0 36.0 2.0 39.0 72-73 26.470560369161287 34.0 21.0 35.0 2.0 38.0 74-75 25.935227412189192 33.0 18.5 35.0 2.0 37.0 76-77 25.309219214059546 33.0 10.0 35.0 2.0 36.5 78-79 24.837782577747234 33.0 4.5 35.0 2.0 36.0 80-81 24.440453106207507 32.5 2.0 35.0 2.0 36.0 82-83 23.932574065437766 32.0 2.0 35.0 2.0 35.0 84-85 23.40236862129059 32.0 2.0 35.0 2.0 35.0 86-87 22.69908691490145 31.0 2.0 35.0 2.0 35.0 88-89 21.986863356324093 30.5 2.0 34.5 2.0 35.0 90-91 21.282747109791117 29.5 2.0 34.0 2.0 35.0 92-93 20.65770354188655 29.0 2.0 34.0 2.0 35.0 94-95 19.958047176063424 28.5 2.0 34.0 2.0 35.0 96-97 18.99922927763187 26.5 2.0 34.0 2.0 35.0 98-99 17.926778920497778 24.5 2.0 33.5 2.0 35.0 100-101 15.581264573770895 9.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.028079249783480975 1101 2 -0.07345074294290299 1101 3 -0.3615579200137482 1101 4 0.10540999788150174 1101 5 0.15857407492284636 1101 6 0.05704182545684944 1101 7 0.17315878295371334 1101 8 0.28319341147911103 1101 9 0.1186198908796996 1101 10-11 0.20557082741903088 1101 12-13 0.27446937615741973 1101 14-15 0.35202380533267785 1101 16-17 0.13580367453955233 1101 18-19 0.21551322039518084 1101 20-21 0.027154340405296296 1101 22-23 0.16013011624025353 1101 24-25 0.2671672475835507 1101 26-27 0.38015786042819855 1101 28-29 0.3278431422393382 1101 30-31 0.3453545521797139 1101 32-33 0.13644962002091887 1101 34-35 0.5459310506962112 1101 36-37 0.8839643077071244 1101 38-39 0.7719389736334676 1101 40-41 1.1755828426039585 1101 42-43 0.7802634788589344 1101 44-45 0.6853514673103689 1101 46-47 0.3464007300062022 1101 48-49 0.6041069770434255 1101 50-51 0.525586767207141 1101 52-53 0.839222256408501 1101 54-55 0.6728455946156018 1101 56-57 0.8973217136837235 1101 58-59 1.0286256874730135 1101 60-61 1.1997661562345314 1101 62-63 0.8854497951707891 1101 64-65 1.1540627711024563 1101 66-67 0.9413600753950853 1101 68-69 1.3594619421289238 1101 70-71 1.3085422455142783 1101 72-73 0.9168551657527892 1101 74-75 1.037891729078492 1101 76-77 0.72074958363633 1101 78-79 1.4316623976309444 1101 80-81 1.6758962468994767 1101 82-83 1.625687023073798 1101 84-85 2.044550121956391 1101 86-87 2.354909929004851 1101 88-89 2.284184884299634 1101 90-91 2.289954121455999 1101 92-93 1.7506574456507131 1101 94-95 1.7657438509466843 1101 96-97 2.124899733146087 1101 98-99 2.2095632392015254 1101 100-101 2.4205571389518905 1102 1 0.3201586356790713 1102 2 0.45450280131020193 1102 3 0.19509005755933728 1102 4 0.2169037904325677 1102 5 -0.031594126919365806 1102 6 0.08349356754735737 1102 7 0.24198137005824094 1102 8 0.18397434860366246 1102 9 0.5429090378560701 1102 10-11 0.319807911920428 1102 12-13 0.23169805056671322 1102 14-15 0.5087018189490209 1102 16-17 0.6723575391771135 1102 18-19 0.5057615183527275 1102 20-21 0.5606345166167515 1102 22-23 0.4276411294561058 1102 24-25 0.41528498887310406 1102 26-27 0.6995010722824233 1102 28-29 0.8886961658676924 1102 30-31 0.8782440195405457 1102 32-33 1.1151340413264847 1102 34-35 0.9448597651535593 1102 36-37 1.0258545760291113 1102 38-39 0.973060319248205 1102 40-41 1.3225892502931913 1102 42-43 1.5506879882702336 1102 44-45 1.8638456603420082 1102 46-47 1.8209201533141837 1102 48-49 1.526784189698617 1102 50-51 1.1538907319648501 1102 52-53 1.412189817481785 1102 54-55 1.6899060671826795 1102 56-57 1.855401409318489 1102 58-59 1.7713769890348878 1102 60-61 1.4506171774600034 1102 62-63 1.1785214811940143 1102 64-65 1.237312670982309 1102 66-67 1.4658895107174708 1102 68-69 1.4316786021209182 1102 70-71 1.9099939876047856 1102 72-73 2.0748447532577927 1102 74-75 1.9265581287581064 1102 76-77 2.106542535178182 1102 78-79 2.257303166553651 1102 80-81 2.2280388179847783 1102 82-83 2.3373309958411177 1102 84-85 2.7563743109832224 1102 86-87 2.448482215748946 1102 88-89 2.6352761938711176 1102 90-91 2.8517082263819127 1102 92-93 2.9289513983940054 1102 94-95 3.2432969146230946 1102 96-97 3.078724322653496 1102 98-99 2.757230439842292 1102 100-101 2.6128679118794036 1103 1 0.34414297243873904 1103 2 0.4401856206934589 1103 3 0.4509841675283397 1103 4 0.41610023357173986 1103 5 0.43811952946830246 1103 6 0.4545165729316025 1103 7 0.35405103884597366 1103 8 0.4995233778090764 1103 9 0.2758589481187528 1103 10-11 0.3290977634459651 1103 12-13 0.22211247380051446 1103 14-15 0.7658706066794849 1103 16-17 0.7166959304318041 1103 18-19 0.48234823723019815 1103 20-21 0.7947469329978887 1103 22-23 0.5365190051291364 1103 24-25 0.5753743182906206 1103 26-27 0.6453935506638899 1103 28-29 0.30915627355246755 1103 30-31 0.47309024241540243 1103 32-33 1.0500438961151914 1103 34-35 0.7759394682046263 1103 36-37 0.49419158043439637 1103 38-39 0.9712655729600641 1103 40-41 1.0234784654995863 1103 42-43 0.889691088286547 1103 44-45 0.9326578646167576 1103 46-47 1.0065438276493026 1103 48-49 1.3433325662690123 1103 50-51 1.4677163968367708 1103 52-53 1.5334983506845923 1103 54-55 1.6315156282856371 1103 56-57 1.7793082456702578 1103 58-59 1.6798041386514662 1103 60-61 1.7012392202075972 1103 62-63 1.7471501318711269 1103 64-65 1.6668994714391552 1103 66-67 2.655796102331113 1103 68-69 2.8313053764723612 1103 70-71 2.945368844840875 1103 72-73 2.5180757044733255 1103 74-75 2.8468142880010525 1103 76-77 3.163342176228923 1103 78-79 3.8207617242302696 1103 80-81 3.6260645970678276 1103 82-83 3.6447526796394527 1103 84-85 3.485795913202182 1103 86-87 3.004404878499802 1103 88-89 2.8405401031548543 1103 90-91 2.3590197780216613 1103 92-93 2.3264571089503754 1103 94-95 2.3423852650880974 1103 96-97 1.8884098341561888 1103 98-99 1.6782921954304832 1103 100-101 1.888402256797006 1104 1 -0.2606879454356559 1104 2 -0.6011187271326293 1104 3 -0.2692654298951709 1104 4 -0.21316707721730666 1104 5 -0.24557612270560725 1104 6 -0.37833366861429596 1104 7 -0.16458825261941 1104 8 -0.04427694425211115 1104 9 -0.03533267829025988 1104 10-11 -0.1964054571659517 1104 12-13 -0.3721709400481146 1104 14-15 -0.47179042391633175 1104 16-17 -0.4117259697292255 1104 18-19 -0.3457516017392024 1104 20-21 -0.3032804422034019 1104 22-23 -0.21921770984670275 1104 24-25 -0.5097102227721777 1104 26-27 -0.6577868036034218 1104 28-29 -0.15437029649584133 1104 30-31 -0.05454663359103762 1104 32-33 -0.33459781080912165 1104 34-35 0.11678085306775188 1104 36-37 -0.11504755000039069 1104 38-39 0.12134608825797244 1104 40-41 0.5033496410229361 1104 42-43 0.747259526289767 1104 44-45 1.1981973171127365 1104 46-47 0.668238674670242 1104 48-49 0.3696208110355208 1104 50-51 0.3853693759027941 1104 52-53 0.3939653394124498 1104 54-55 0.23075073295552073 1104 56-57 0.547222899454475 1104 58-59 0.9019458455757778 1104 60-61 0.937612006036904 1104 62-63 1.090588135091739 1104 64-65 0.7478374746597645 1104 66-67 0.21724940345832877 1104 68-69 -0.385300903720875 1104 70-71 -0.14531150942643478 1104 72-73 0.13246781397413088 1104 74-75 -0.05479601795708078 1104 76-77 -0.1346851989723632 1104 78-79 0.41160310909339515 1104 80-81 0.7755009899824792 1104 82-83 0.4113589598326932 1104 84-85 1.4031469599010542 1104 86-87 0.7191391780167109 1104 88-89 0.7649161095960757 1104 90-91 0.11366953647576494 1104 92-93 0.4259538883384586 1104 94-95 0.7912379220929253 1104 96-97 0.23339775686150332 1104 98-99 0.27863438544658337 1104 100-101 0.5808338187937849 1105 1 0.4369892433672007 1105 2 0.4139299129309002 1105 3 0.27486798285050895 1105 4 0.5324751701521535 1105 5 0.2708820865459245 1105 6 0.3981999823684319 1105 7 0.1949313026714421 1105 8 0.4016035401632152 1105 9 0.681363768007138 1105 10-11 0.48774185274074 1105 12-13 0.6428379938411055 1105 14-15 1.1001869435643101 1105 16-17 0.8880556463120683 1105 18-19 0.6408971971490232 1105 20-21 0.6559214636929696 1105 22-23 0.6981438148703916 1105 24-25 0.7113141965250236 1105 26-27 0.5585721402123411 1105 28-29 0.8312262887731663 1105 30-31 0.8483122109592856 1105 32-33 1.4005322269117428 1105 34-35 1.7145176011320729 1105 36-37 1.2598879274663588 1105 38-39 1.4261108291751867 1105 40-41 1.5393333614914724 1105 42-43 1.4065606976219058 1105 44-45 1.2326324967831752 1105 46-47 1.5331483431236848 1105 48-49 0.9182310949346686 1105 50-51 1.0946506941477807 1105 52-53 0.727308599289362 1105 54-55 1.1227003061141474 1105 56-57 0.664455379105064 1105 58-59 0.5350806480375603 1105 60-61 0.736659057853462 1105 62-63 0.6949558142658496 1105 64-65 0.5487804962996314 1105 66-67 0.36010229208250877 1105 68-69 0.35822698986657997 1105 70-71 0.21556798233453378 1105 72-73 0.5542565771268038 1105 74-75 0.6802744605023214 1105 76-77 0.5256582594354207 1105 78-79 0.7125362041896821 1105 80-81 0.4604126437446432 1105 82-83 0.19089042697583025 1105 84-85 0.49296232619051494 1105 86-87 0.5253333412090875 1105 88-89 0.36429073735069295 1105 90-91 0.3828972513854296 1105 92-93 0.297315810117297 1105 94-95 1.1516508663057508 1105 96-97 1.0283451461805377 1105 98-99 1.2749119316050113 1105 100-101 1.5770120995164394 1106 1 0.07314026241164129 1106 2 -0.1268875500382478 1106 3 -0.16565991557915893 1106 4 -0.06210663183024678 1106 5 0.14238782215122825 1106 6 0.30682009596682747 1106 7 0.21595257452799643 1106 8 -0.0449662337537049 1106 9 0.2862473853364591 1106 10-11 -0.2331764009623427 1106 12-13 -0.2183798477893646 1106 14-15 -0.24875224788239336 1106 16-17 -0.5555488753495865 1106 18-19 -0.15089476186646067 1106 20-21 -0.3557724888630034 1106 22-23 -0.2776747618085267 1106 24-25 -0.16259993645191884 1106 26-27 -0.14074014400639356 1106 28-29 -0.044163509645365195 1106 30-31 -0.2657873546939058 1106 32-33 -0.1122754354114548 1106 34-35 0.04925699304654074 1106 36-37 -0.41891817566316547 1106 38-39 -0.04352665829558333 1106 40-41 0.1788533525817897 1106 42-43 0.5419042770851021 1106 44-45 0.33385479325271206 1106 46-47 0.534039310937473 1106 48-49 0.021291012520144648 1106 50-51 -0.2609985986465162 1106 52-53 0.06477658013355736 1106 54-55 0.17295645936060922 1106 56-57 0.45907337665489933 1106 58-59 -0.3941570176267639 1106 60-61 0.0500987504695658 1106 62-63 0.12192429627943824 1106 64-65 0.35112485535966087 1106 66-67 0.7246194988984129 1106 68-69 0.5597945363639596 1106 70-71 0.18514978431693763 1106 72-73 -0.027712461741668193 1106 74-75 0.29814671799201875 1106 76-77 0.08904226656316183 1106 78-79 0.19164022468194375 1106 80-81 0.12900045975978713 1106 82-83 0.2577269343820028 1106 84-85 0.3300822727324437 1106 86-87 0.3614509489605062 1106 88-89 0.7623445295324522 1106 90-91 1.2951647644715223 1106 92-93 0.8099146518591382 1106 94-95 0.6210099263347075 1106 96-97 0.48376891274697087 1106 98-99 0.3402727735695983 1106 100-101 0.9892378484862565 1107 1 0.33503550431488094 1107 2 0.2776173146825265 1107 3 0.3322076040796418 1107 4 0.5508148662371966 1107 5 0.5030102398508163 1107 6 0.4089792323122907 1107 7 0.47601521613801623 1107 8 0.46612932802158724 1107 9 0.566235390134544 1107 10-11 0.4456354076475506 1107 12-13 0.4569333602607486 1107 14-15 0.49883205173108536 1107 16-17 0.42267650116647104 1107 18-19 0.419810289446346 1107 20-21 0.7342581655419025 1107 22-23 0.7285727391910655 1107 24-25 0.728493626123047 1107 26-27 0.5833868718539392 1107 28-29 0.2617974393083884 1107 30-31 0.2905234543158244 1107 32-33 0.34061007705022917 1107 34-35 0.620012024367341 1107 36-37 0.5457626186857638 1107 38-39 0.2569364897785249 1107 40-41 -0.1251250560547561 1107 42-43 -0.2012318018166681 1107 44-45 0.013866122054523089 1107 46-47 0.014247227770738391 1107 48-49 -0.19727156244987043 1107 50-51 -0.4573367300606179 1107 52-53 0.1925754605813772 1107 54-55 0.5836255250676636 1107 56-57 0.5726446148262987 1107 58-59 0.008431946787467837 1107 60-61 0.6038272590661293 1107 62-63 -0.035712648942475056 1107 64-65 -0.5076982820520435 1107 66-67 -0.18668264690992942 1107 68-69 -0.22527476924712886 1107 70-71 0.03602610045714627 1107 72-73 -0.1330703185989286 1107 74-75 -0.4089537751440595 1107 76-77 -0.27829413221066446 1107 78-79 -0.03871763217531665 1107 80-81 0.47336271485774617 1107 82-83 0.29422900022233023 1107 84-85 0.9133156460497815 1107 86-87 1.0622373010863235 1107 88-89 0.2710577109265593 1107 90-91 0.050162765271203114 1107 92-93 0.2073390154470367 1107 94-95 0.22478508293873745 1107 96-97 -0.13948178697313907 1107 98-99 -0.0034397414244047297 1107 100-101 0.3836967315996773 1108 1 0.3677308060824416 1108 2 0.3801297547716693 1108 3 0.32820845885877503 1108 4 0.5893104549663164 1108 5 0.30054969706606727 1108 6 0.416031160144513 1108 7 0.2988317133752503 1108 8 0.5636657324542256 1108 9 0.5927945988532883 1108 10-11 0.6643062260985673 1108 12-13 0.6604013213072406 1108 14-15 0.7026205549517783 1108 16-17 0.8088229736761647 1108 18-19 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-3.068050903249194 2316 62-63 -2.961183201462209 2316 64-65 -3.4557857137460317 2316 66-67 -3.111950811249134 2316 68-69 -3.104651413651098 2316 70-71 -3.441205229233198 2316 72-73 -3.6431897276254404 2316 74-75 -3.514296823110058 2316 76-77 -3.2517812805612003 2316 78-79 -4.073528399226518 2316 80-81 -4.0251699708334066 2316 82-83 -4.3174386783852405 2316 84-85 -4.463278160871894 2316 86-87 -4.72399018322859 2316 88-89 -4.71887347709206 2316 90-91 -3.9534802219783423 2316 92-93 -3.4644379527780167 2316 94-95 -3.460855475652643 2316 96-97 -3.439522264609245 2316 98-99 -3.469084347779397 2316 100-101 -3.4140668790054622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 880.0 3 127.0 4 118.0 5 125.0 6 131.0 7 177.0 8 229.0 9 287.0 10 431.0 11 755.0 12 1396.0 13 2262.0 14 2516.0 15 3527.0 16 4991.0 17 7057.0 18 5574.0 19 2750.0 20 2515.0 21 2546.0 22 2742.0 23 3155.0 24 3264.0 25 3600.0 26 3805.0 27 4228.0 28 4736.0 29 5548.0 30 6544.0 31 7819.0 32 9489.0 33 12087.0 34 16653.0 35 26216.0 36 21715.0 37 20611.0 38 12124.0 39 975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.605447838741604 16.77640812989712 14.691730730400437 13.926413300960833 2 14.718810760256948 24.22189953483793 40.585267406659945 20.47402229824518 3 21.96290206657543 34.35250913172327 31.38900648820014 12.295582313501166 4 10.49121097114954 18.22339048294126 38.19769531038489 33.087703235524316 5 9.672560447349769 42.054717649838544 30.76218791840592 17.510533984405765 6 21.803468208092486 38.211290124215964 19.087935063337845 20.89730660435371 7 20.852660937661405 43.796392399148026 18.505310069702354 16.845636593488216 8 25.37165120374652 28.994205094400883 19.84632185830521 25.787821843547388 9 30.594063302472474 17.528704532708904 20.934877360068477 30.942354804750146 10-11 23.272225488194533 28.37345559821817 23.14333573566778 25.21098317791951 12-13 32.76330710327134 27.910786631277812 24.495056957525993 14.830849307924856 14-15 27.817420200663634 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13.733301855240118 40-41 14.783592366712572 26.677405075742673 44.713013968129054 13.825988589415699 42-43 13.94708234750144 29.819918641199823 41.7844336119077 14.44856539939104 44-45 14.45706682538238 30.364955963767564 39.07751409872675 16.100463112123307 46-47 14.015298718547854 29.10962441891925 36.879012224217234 19.996064638315666 48-49 15.615437595316575 28.857676981354846 36.7973631131008 18.729522310227775 50-51 18.01637158781773 29.69022870045602 36.23714205599201 16.05625765573424 52-53 14.876811665502347 30.058150403904243 37.4964332451074 17.568604685486015 54-55 13.620040829377475 29.554074329143816 38.06503185183363 18.76085298964508 56-57 15.997452486162034 31.775563047279004 33.49587258496426 18.731111881594703 58-59 15.200517305389663 29.210052050166823 35.081369875812285 20.508060768631232 60-61 16.412441812713784 27.44644802427575 36.2483739527518 19.89273621025867 62-63 20.654611442790962 27.02589725683823 34.99055777950448 17.328933520866325 64-65 19.340861473692495 27.933466452888467 37.42118568210657 15.304486391312475 66-67 17.351116488023667 28.4675553326104 37.646584390068774 16.534743789297156 68-69 16.640068458623595 30.78818400222294 36.390283006572915 16.18146453258055 70-71 16.35858405773936 30.25660572229531 34.168029410679814 19.216780809285513 72-73 16.209888445117752 28.65430774981801 33.784209180160154 21.351594624904084 74-75 16.654820200151995 28.812492160426572 35.899958434889115 18.63272920453232 76-77 17.613422561295035 26.981374769736277 37.19069648474295 18.214506184225737 78-79 15.47667063441315 26.71637826724248 39.95455126754744 17.852399830796926 80-81 14.593339678370555 29.952264661831652 37.845441779364855 17.60895388043294 82-83 13.933513169251501 29.384238209640813 35.67046256519462 21.011786055913067 84-85 15.307083438862401 26.636356259574455 35.4703071940907 22.586253107472444 86-87 17.08471658674536 26.57764662486167 36.31181605803517 20.0258207303578 88-89 18.487349492405762 24.09965963642087 37.385889667112615 20.027101204060756 90-91 15.745738828488307 25.524730902500263 39.14720008262898 19.58233018638245 92-93 14.399077357386306 28.399785569200652 37.73908296406317 19.46205410934987 94-95 14.221776295727958 28.985304098916988 36.27472678805048 20.51819281730457 96-97 14.716898536966855 27.04041355463687 34.43575221151883 23.80693569687744 98-99 16.44524890438786 26.18181013324414 34.64913359336189 22.723807369006106 100-101 18.81239242685026 25.11261372018687 36.71035161052373 19.364642242439146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1688.0 1 1220.5 2 1132.5 3 1472.5 4 1101.0 5 957.0 6 1921.0 7 4261.0 8 6705.0 9 6837.5 10 4661.5 11 2839.0 12 2385.0 13 2244.0 14 2016.5 15 1747.0 16 1527.5 17 1361.5 18 1245.5 19 1216.0 20 1155.0 21 1141.0 22 1204.0 23 1253.0 24 1311.0 25 1376.5 26 1523.0 27 1701.5 28 1831.0 29 1936.0 30 2130.5 31 2313.0 32 2563.5 33 2927.5 34 3221.5 35 3364.0 36 3594.5 37 3837.0 38 3828.0 39 3803.0 40 3732.5 41 3714.0 42 3674.5 43 3665.0 44 4159.0 45 4341.0 46 4842.5 47 7452.5 48 18976.0 49 19278.0 50 9218.0 51 6118.5 52 2855.5 53 1739.5 54 1424.0 55 1336.0 56 1423.5 57 1531.5 58 1474.0 59 1523.0 60 1651.0 61 1779.0 62 1552.0 63 801.5 64 335.5 65 263.0 66 222.0 67 150.0 68 104.5 69 94.0 70 96.5 71 81.0 72 59.0 73 78.5 74 85.0 75 52.5 76 35.0 77 23.0 78 15.0 79 11.0 80 6.5 81 7.0 82 6.0 83 2.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3200706904592425 2 0.2699982818291156 3 0.2783436832674701 4 0.2719619056969637 5 0.2714709997300017 6 0.21108956579367222 7 0.2022532583883557 8 0.20765322402493802 9 0.20961684789278612 10-11 0.21133501877715322 12-13 0.21575317247981152 14-15 0.21084411281019122 16-17 0.20495324120664687 18-19 0.19979872855354555 20-21 0.19415330993348223 22-23 0.2022532583883557 24-25 0.2022532583883557 26-27 0.20937139490930512 28-29 0.21526226651284947 30-31 0.21378954861196336 32-33 0.21182592474411527 34-35 0.21158047176063427 36-37 0.20127144645443168 38-39 0.19096242114822906 40-41 0.18899879728038094 42-43 0.2002896345205076 44-45 0.19955327557006458 46-47 0.20618050612405195 48-49 0.21354409562848237 50-51 0.20912594192582412 52-53 0.21550771949633046 54-55 0.20618050612405195 56-57 0.1813897547924695 58-59 0.16862619965145675 60-61 0.18384428462727964 62-63 0.17819886600721632 64-65 0.18605336147860876 66-67 0.17991703689158342 68-69 0.18188066075943152 70-71 0.18531700252816574 72-73 0.19488966888392528 74-75 0.20102599347095063 76-77 0.19906236960310253 78-79 0.19587148081784936 80-81 0.19464421590044426 82-83 0.18237156672639357 84-85 0.17893522495765937 86-87 0.1865442674455708 88-89 0.19292604501607716 90-91 0.189735156230824 92-93 0.18458064357772266 94-95 0.1880169853464569 96-97 0.1936624039665202 98-99 0.1934169509830392 100-101 0.1742716182715201 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 203705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.15723718121794 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82150043115837 26.923246852065486 2 10.885311871227364 7.436243587540807 3 4.006898534061512 4.105937507670405 4 1.8970968669157804 2.59198350555951 5 1.016096579476861 1.7353525932107705 6 0.6467375682667433 1.3254461107974767 7 0.4857717734981316 1.1614835178321592 8 0.31474561655648176 0.8600672541174738 9 0.2630066110951423 0.8085221275864607 >10 1.3466513365909745 8.385164821678408 >50 0.12647312446105202 2.90174517071255 >100 0.14084507042253522 9.43864902677892 >500 0.021557918942224776 4.953732112613829 >1k 0.022995113538373097 16.593112589283525 >5k 0.004311583788444956 10.77931322255222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9699 4.761296973564714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6875 3.374978522863945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5384 2.643037726123561 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4498 2.208095039395204 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4054 1.990132790064063 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3507 1.7216072261358337 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2744 1.3470459733438058 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2300 1.1290837240126654 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1854 0.9101396627476006 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1851 0.9086669448467146 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1832 0.8993397314744361 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1673 0.8212856827274736 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.7879040769740556 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.7731768979651948 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1404 0.6892319776146879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1386 0.6803956702093714 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1200 0.5890871603544341 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1171 0.5748508873125353 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1147 0.5630691441054466 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 953 0.4678333865148131 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 853 0.41874278981861024 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.40254289290886336 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 816 0.4005792690410152 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 808 0.396652021305319 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 706 0.34657961267519205 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 682 0.33479786946810336 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 603 0.29601629807810315 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 589 0.28914361454063475 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 585 0.28717999067278666 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 572 0.28079821310228026 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 558 0.27392552956481186 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 536 0.2631255982916472 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 506 0.2483984192827864 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 504 0.2474166073488623 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 489 0.2400530178444319 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.2380893939765838 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 481 0.2361257701087357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 436 0.2140350015954444 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 430 0.21108956579367222 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 399 0.19587148081784936 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 387 0.18998060921430499 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 353 0.17328980633759603 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 346 0.16985346456886183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 342 0.16788984070101373 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 336 0.16494440489924156 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 326 0.16003534522962126 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 323 0.1585626273287352 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 309 0.1516899437912668 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 308 0.15119903782430474 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 308 0.15119903782430474 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.14776269605557057 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 293 0.14383544831987433 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 278 0.1364718588154439 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 277 0.13598095284848186 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 273 0.13401732898063376 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 268 0.1315627991458236 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 266 0.13058098721189956 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 240 0.11781743207088682 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 237 0.11634471417000074 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 234 0.11487199626911465 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 221 0.10849021869860828 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 217 0.10652659483076016 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 217 0.10652659483076016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 213 0.10456297096291206 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 210 0.10309025306202597 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 208 0.1021084411281019 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.001227264917405071 0.0 22-23 0.0 0.0 0.0 0.004172700719177242 0.0 24-25 0.0 0.0 0.0 0.005645418620063326 0.0 26-27 0.0 0.0 0.0 0.008099948454873468 0.0 28-29 0.0 0.0 0.0 0.012763555141012739 0.0 30-31 0.0 0.0 0.0 0.023563486414177364 0.0 32-33 0.0 0.0 0.0 0.029699811001202718 0.0 34-35 0.0 0.0 0.0 0.038045212439557205 0.0 36-37 0.0 0.0 0.0 0.04810878476227878 0.0 38-39 0.0 0.0 0.0 0.05645418620063327 0.0 40-41 0.0 0.0 0.0 0.07461770697822832 0.0 42-43 0.0 0.0 0.0 0.09179941582189932 0.0 44-45 0.0 0.0 0.0 0.1293537222944945 0.0 46-47 0.0 0.0 0.0 0.22017132618246976 0.0 48-49 0.0 0.0 0.0 0.2798164011683562 0.0 50-51 0.0 0.0 0.0 0.34952504847696425 0.0 52-53 0.0 0.0 0.0 0.4354335926953192 0.0 54-55 0.0 0.0 0.0 0.5230603077980414 0.0 56-57 0.0 0.0 0.0 0.6133870057190545 0.0 58-59 0.0 0.0 0.0 0.7670405733781693 0.0 60-61 0.0 0.0 0.0 0.9847573697258291 0.0 62-63 0.0 0.0 0.0 1.1713016371713998 0.0 64-65 0.0 0.0 0.0 1.296237205763236 0.0 66-67 0.0 0.0 0.0 1.4790996784565915 0.0 68-69 0.0 0.0 0.0 1.6806165778945044 0.0 70-71 0.0 0.0 0.0 1.8590608968852016 0.0 72-73 0.0 0.0 0.0 2.180849758228811 0.0 74-75 0.0 0.0 0.0 2.45403892884318 0.0 76-77 0.0 0.0 0.0 2.6572739991654597 0.0 78-79 0.0 0.0 0.0 2.903708794580398 0.0 80-81 0.0 0.0 0.0 3.1506344959622985 0.0 82-83 0.0 0.0 0.0 3.4383053926020475 0.0 84-85 0.0 0.0 0.0 3.9655384011192654 0.0 86-87 0.0 0.0 0.0 4.642497729559903 0.0 88-89 0.0 0.0 0.0 5.14763996956383 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 25 5.2569012E-5 76.02325 6 GATAGTC 25 5.2569012E-5 76.02325 7 CATATAG 20 0.0020650595 71.30691 3 AGTGGAG 20 0.0020690998 71.27179 8 ACCAGGC 20 0.0020690998 71.27179 7 TAGATAG 35 3.3907763E-6 67.91134 5 GTCATAG 25 0.004999511 57.04553 1 CGCTCCG 20 5.3791946E-4 47.50868 34-35 GACCGTC 20 5.3824607E-4 47.50283 20-21 CGACCGT 20 5.3824607E-4 47.50283 18-19 TCGACCG 20 5.3824607E-4 47.50283 18-19 TACCTCC 110 1.5934347E-9 38.89468 2 GGGATGG 135 7.2759576E-12 38.71554 7 ATAGTCA 50 0.0016102626 38.011623 8 TAGTCAA 50 0.0016102626 38.011623 9 GCTCCGC 25 0.0016131371 38.006943 34-35 GTTCGAC 25 0.0016141131 38.002266 16-17 TCCGCCA 25 0.0016150896 37.99759 36-37 ATGGGGC 90 5.631864E-7 36.973953 5 CTACCTC 120 3.7325663E-9 35.653454 1 >>END_MODULE