##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 203705 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04102010259935 31.0 31.0 34.0 31.0 34.0 2 32.176534694779214 33.0 31.0 34.0 30.0 34.0 3 32.224893841584645 34.0 31.0 34.0 30.0 34.0 4 35.870754277018236 37.0 35.0 37.0 35.0 37.0 5 35.7562897327017 37.0 35.0 37.0 35.0 37.0 6 35.97361871333546 37.0 35.0 37.0 35.0 37.0 7 35.932829336540586 37.0 35.0 37.0 35.0 37.0 8 36.01162956235733 37.0 35.0 37.0 35.0 37.0 9 37.52370830367443 39.0 37.0 39.0 35.0 39.0 10-11 37.51003166343487 39.0 37.0 39.0 35.0 39.0 12-13 37.3865958125721 39.0 37.0 39.0 34.0 39.0 14-15 38.14573525441202 40.0 37.0 41.0 33.0 41.0 16-17 38.208247220244964 40.0 37.5 41.0 33.5 41.0 18-19 38.46169215286812 40.0 38.0 41.0 34.0 41.0 20-21 38.546881519844874 40.0 38.0 41.0 34.0 41.0 22-23 38.40848776416877 40.0 37.5 41.0 34.5 41.0 24-25 38.250315407083775 40.0 37.0 41.0 34.0 41.0 26-27 37.855187648805874 40.0 36.5 41.0 33.5 41.0 28-29 37.577212145013625 39.0 35.5 41.0 33.0 41.0 30-31 37.29483566922756 39.0 35.0 41.0 33.0 41.0 32-33 36.86555558282811 38.0 35.0 41.0 33.0 41.0 34-35 36.57716305441693 38.0 35.0 41.0 33.0 41.0 36-37 36.18347855968189 38.0 35.0 40.0 32.0 41.0 38-39 35.80197589651702 37.5 35.0 40.0 31.0 41.0 40-41 35.36764438771753 37.0 35.0 40.0 30.5 41.0 42-43 34.9884710733659 37.0 35.0 40.0 29.5 41.0 44-45 34.38167448025331 37.0 34.5 40.0 24.5 41.0 46-47 33.48689526521195 36.5 33.0 40.0 19.0 41.0 48-49 32.76646375886699 36.0 33.0 40.0 4.5 41.0 50-51 32.37061682334749 36.0 33.0 40.0 2.0 41.0 52-53 31.911315382538476 35.0 33.0 40.0 2.0 41.0 54-55 31.69655138558209 35.0 33.0 40.0 2.0 41.0 56-57 31.509616847892786 35.0 33.0 40.0 2.0 41.0 58-59 31.29375076704057 35.0 32.0 39.5 2.0 41.0 60-61 30.843555631918704 35.0 31.0 39.0 2.0 40.5 62-63 30.29564811860288 35.0 31.0 38.5 2.0 40.0 64-65 29.721452590756243 35.0 29.5 38.0 2.0 40.0 66-67 29.252892663410325 35.0 29.0 37.0 2.0 39.5 68-69 28.839814928450455 35.0 29.0 37.0 2.0 39.0 70-71 28.39025797108564 34.5 27.5 36.0 2.0 39.0 72-73 27.863358778625955 34.0 26.5 35.0 2.0 38.0 74-75 27.341474190618786 34.0 26.0 35.0 2.0 37.0 76-77 26.055047740605286 32.5 24.0 34.5 2.0 36.0 78-79 26.393007044500624 33.0 24.0 35.0 2.0 36.0 80-81 26.237738396210204 33.0 23.5 35.0 2.0 36.0 82-83 25.90833312878918 33.0 20.5 35.0 2.0 35.5 84-85 25.494960850249136 33.0 19.0 35.0 2.0 35.0 86-87 24.90949166687121 33.0 9.0 35.0 2.0 35.0 88-89 24.27331189710611 32.5 2.0 35.0 2.0 35.0 90-91 23.58510345843254 31.5 2.0 34.5 2.0 35.0 92-93 23.22425811835743 31.0 2.0 34.0 2.0 35.0 94-95 22.833165607127953 31.0 2.0 34.0 2.0 35.0 96-97 22.19995336393314 31.0 2.0 34.0 2.0 35.0 98-99 21.3964532043887 30.5 2.0 34.0 2.0 35.0 100-101 19.608009130850988 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2391028953091947 1101 2 0.2912607977293291 1101 3 0.34233567049732017 1101 4 0.3044723340990103 1101 5 0.1383854018412407 1101 6 0.13544769137747892 1101 7 0.13288758128762623 1101 8 0.029904875499802586 1101 9 0.35823471578205357 1101 10-11 0.265382682084244 1101 12-13 0.16994636229202342 1101 14-15 0.2141281025470292 1101 16-17 0.37254379956617356 1101 18-19 0.40163584300584887 1101 20-21 0.3165445054090057 1101 22-23 0.42892397966394924 1101 24-25 0.4881522643112248 1101 26-27 0.0426536723027553 1101 28-29 0.42939326598622785 1101 30-31 0.3178293226382891 1101 32-33 0.40685443064360527 1101 34-35 0.39142631518293314 1101 36-37 0.2553142555426433 1101 38-39 0.238561547821611 1101 40-41 0.14111660587442287 1101 42-43 -0.07311898320431709 1101 44-45 -0.10926163981523729 1101 46-47 0.5755654153280716 1101 48-49 0.6540836920022599 1101 50-51 1.2364196979855464 1101 52-53 1.1268086103760915 1101 54-55 1.4277653745440801 1101 56-57 1.4143661207966325 1101 58-59 1.6593997850036963 1101 60-61 1.8525977462092804 1101 62-63 1.8915205264681987 1101 64-65 1.9761469472893545 1101 66-67 1.8921342502080094 1101 68-69 1.9954374189964774 1101 70-71 1.8905487421046026 1101 72-73 2.053747187143575 1101 74-75 1.7946203188024619 1101 76-77 1.7456853004145252 1101 78-79 2.0537783514580603 1101 80-81 2.2913987684801853 1101 82-83 2.183171721272977 1101 84-85 2.83046173218246 1101 86-87 2.644518883605887 1101 88-89 2.6865804093833 1101 90-91 2.1736087923361467 1101 92-93 1.430779015556709 1101 94-95 1.4323803363038614 1101 96-97 2.093179474492697 1101 98-99 2.603632922349451 1101 100-101 2.784793674600426 1102 1 0.1435682537393106 1102 2 0.34060000477779084 1102 3 0.13280423277205244 1102 4 0.02245391199247848 1102 5 0.049799098276963605 1102 6 -0.10510096701258931 1102 7 0.18036455365446358 1102 8 0.26268421070204795 1102 9 0.3409740029266288 1102 10-11 0.0950282567738725 1102 12-13 -0.0024905620171509213 1102 14-15 0.31018336886661046 1102 16-17 0.12224343913365487 1102 18-19 0.1444972767263124 1102 20-21 0.33403549459603 1102 22-23 0.33625277421741373 1102 24-25 0.11351269683024867 1102 26-27 -0.1753979896916391 1102 28-29 -0.12042651775426094 1102 30-31 0.13986576637076809 1102 32-33 -0.10543031104642608 1102 34-35 0.16229135322858923 1102 36-37 -0.0067201857869534365 1102 38-39 0.002638440092333383 1102 40-41 0.10284067475707559 1102 42-43 0.12929391227024922 1102 44-45 0.0025425251827613238 1102 46-47 -0.5033693063379232 1102 48-49 -0.8505118226152817 1102 50-51 -0.46086704610736007 1102 52-53 -0.7402518621909842 1102 54-55 -0.5552041889320947 1102 56-57 -0.6580207434404493 1102 58-59 -0.5375966106711196 1102 60-61 -0.6067034151843949 1102 62-63 -0.6512708231513429 1102 64-65 -0.31176855130896186 1102 66-67 -0.7105489696558287 1102 68-69 -0.7531909350283499 1102 70-71 -0.9290848181677234 1102 72-73 -0.8404358324799723 1102 74-75 -0.8378486439528423 1102 76-77 -0.569512937470936 1102 78-79 -0.5551523654021757 1102 80-81 -0.8101230577112446 1102 82-83 -0.6137946383585771 1102 84-85 -0.37659673798173543 1102 86-87 -0.28995248203287716 1102 88-89 -0.7133795425590321 1102 90-91 -0.05897030257775526 1102 92-93 -0.24543243818778393 1102 94-95 -0.40074941940298814 1102 96-97 0.18354791662325098 1102 98-99 0.18853480460815675 1102 100-101 0.21341802381948938 1103 1 0.1463419525482479 1103 2 0.3310756125441472 1103 3 0.04157809473974794 1103 4 0.0571659367926074 1103 5 -0.1139715005156603 1103 6 0.007417388347178644 1103 7 0.022365695765742544 1103 8 0.164585010179934 1103 9 -0.04740501485767368 1103 10-11 0.2003574295589985 1103 12-13 0.15412144646710857 1103 14-15 0.25697322039214754 1103 16-17 -0.19142926440689223 1103 18-19 0.025962442332442492 1103 20-21 0.16351420237869974 1103 22-23 0.1333852591252267 1103 24-25 0.038531052190016624 1103 26-27 0.0631503052993807 1103 28-29 -0.3396303030373389 1103 30-31 -0.06769256288359315 1103 32-33 -0.189273515484345 1103 34-35 0.011502072758695192 1103 36-37 -0.13395342122502996 1103 38-39 -0.08437616261610259 1103 40-41 -0.0323261550683398 1103 42-43 -0.17648598657132197 1103 44-45 -0.207157262710858 1103 46-47 0.16066642542908482 1103 48-49 0.3618698872884565 1103 50-51 0.220636869702723 1103 52-53 0.07739783596532135 1103 54-55 -0.27227671299801415 1103 56-57 -0.2670311856006755 1103 58-59 0.041260054363963405 1103 60-61 -0.3609965298849964 1103 62-63 -0.06757038262270854 1103 64-65 0.431786677929086 1103 66-67 0.4494995700733284 1103 68-69 0.1946798432389052 1103 70-71 0.3364662505221112 1103 72-73 0.41877243966882816 1103 74-75 0.6707987699809159 1103 76-77 0.43827789300712183 1103 78-79 0.6684669036466104 1103 80-81 0.6685031455845625 1103 82-83 1.0172626303638879 1103 84-85 0.872465099185824 1103 86-87 1.3435929576799595 1103 88-89 1.6856965709994611 1103 90-91 1.1386761324034822 1103 92-93 1.199297534075228 1103 94-95 0.795132861556386 1103 96-97 0.8828259391391633 1103 98-99 0.935914067130593 1103 100-101 0.6411488934556466 1104 1 0.03297641309575283 1104 2 0.16952166729454632 1104 3 -0.07545089076749889 1104 4 0.10249604951403057 1104 5 -0.5473853491469072 1104 6 -0.168307249333985 1104 7 0.03585200816509371 1104 8 -0.010213701575295886 1104 9 -0.18721982967249318 1104 10-11 -0.2667319424216785 1104 12-13 -0.06355166142339641 1104 14-15 -0.19298652195890043 1104 16-17 -0.25117161545358613 1104 18-19 -0.12613095541313157 1104 20-21 -0.06655826138941023 1104 22-23 0.10323228005920981 1104 24-25 -0.2279544550563628 1104 26-27 -0.23155581386325963 1104 28-29 -0.26289922413234734 1104 30-31 -0.1715975548320685 1104 32-33 -0.04680169583861016 1104 34-35 -0.1176645939079819 1104 36-37 0.13337749664936638 1104 38-39 0.15654573754492418 1104 40-41 -0.2690612597382298 1104 42-43 -0.2076051492122204 1104 44-45 -0.158767568668992 1104 46-47 0.10619782639526676 1104 48-49 -0.4837621578001148 1104 50-51 -0.5308727921330245 1104 52-53 -0.8528356583986074 1104 54-55 -0.20691841992232796 1104 56-57 -0.4488749859227319 1104 58-59 -0.6925764995812891 1104 60-61 -1.3720069968737256 1104 62-63 -0.7762660347966204 1104 64-65 -1.0555724202995762 1104 66-67 -1.3914625877366547 1104 68-69 -1.927783924877037 1104 70-71 -1.5237793211365052 1104 72-73 -1.24892079704615 1104 74-75 -1.0114073491817273 1104 76-77 -1.1130103517593888 1104 78-79 -1.212229553680281 1104 80-81 -1.1135419429822626 1104 82-83 -0.8349112826795917 1104 84-85 -0.9061983468689263 1104 86-87 -0.8326550294375927 1104 88-89 -0.8254749265850805 1104 90-91 -0.6987232234741327 1104 92-93 -0.5061775061824214 1104 94-95 -0.5388647229846768 1104 96-97 -0.5759904859816096 1104 98-99 -0.03411411322367641 1104 100-101 0.2459596825055641 1105 1 0.25390156695718247 1105 2 0.34331559224987984 1105 3 0.21680640524203199 1105 4 0.2092460991467462 1105 5 0.10127456747012786 1105 6 0.25456350706863873 1105 7 0.12846665974341676 1105 8 0.39530545665380856 1105 9 0.48538291005353784 1105 10-11 0.47880111296843353 1105 12-13 0.25451464788770295 1105 14-15 0.32514927315724407 1105 16-17 -0.013093394290201843 1105 18-19 0.2433647761731379 1105 20-21 0.17207584621431948 1105 22-23 0.13573178373203376 1105 24-25 0.518839271368094 1105 26-27 0.4461094630873177 1105 28-29 0.6005016096973108 1105 30-31 0.5418330586283346 1105 32-33 0.585777220182834 1105 34-35 0.17617102253037586 1105 36-37 0.39620259095152477 1105 38-39 0.25196960095568954 1105 40-41 0.001317934226428008 1105 42-43 0.019543947472307366 1105 44-45 0.8249330467767138 1105 46-47 0.1296542488689596 1105 48-49 -0.2718503055070727 1105 50-51 0.032756352198923366 1105 52-53 0.005036090658368408 1105 54-55 -0.16379751462155667 1105 56-57 -0.31529348899995924 1105 58-59 -0.010712294697430735 1105 60-61 0.06020463703534418 1105 62-63 0.1904204849572011 1105 64-65 0.46235491740143786 1105 66-67 0.6111877993985466 1105 68-69 0.37961135008821856 1105 70-71 0.25620876701120565 1105 72-73 0.06841094804021353 1105 74-75 0.5226506218327671 1105 76-77 0.6367811908254843 1105 78-79 0.49130429583332 1105 80-81 0.1469404870356037 1105 82-83 -0.11629901347173188 1105 84-85 0.03431187747095876 1105 86-87 0.37225860963328117 1105 88-89 0.39812046749869623 1105 90-91 0.30654777116553333 1105 92-93 1.1408246776571644 1105 94-95 1.0263923744939802 1105 96-97 0.6098046301589477 1105 98-99 1.1187844375009668 1105 100-101 1.1032556920350451 1106 1 0.05916941415620158 1106 2 0.23272421236347896 1106 3 0.0710496394551896 1106 4 0.21389583742494978 1106 5 -0.03921991966652172 1106 6 -0.08506228644957048 1106 7 -0.13673547857933244 1106 8 0.04986052960179421 1106 9 0.3176582191079973 1106 10-11 0.17003900137471106 1106 12-13 0.03973571927649999 1106 14-15 0.2724429584228645 1106 16-17 0.41855266874577524 1106 18-19 0.18799947936948058 1106 20-21 0.34237599099659377 1106 22-23 0.2538685472914466 1106 24-25 0.16099040178350776 1106 26-27 0.009006222191892732 1106 28-29 -0.04028522625323916 1106 30-31 -0.26642788357013814 1106 32-33 0.10081230204049518 1106 34-35 -0.296932886590902 1106 36-37 0.2788175814849936 1106 38-39 -0.5971923324444646 1106 40-41 -0.4990718641921035 1106 42-43 -0.08581299650808916 1106 44-45 0.027711752645963372 1106 46-47 1.1813303820686514 1106 48-49 1.2507023172794192 1106 50-51 0.9088143764777925 1106 52-53 1.3102343309969378 1106 54-55 1.2757698091338767 1106 56-57 1.078723105275575 1106 58-59 0.9993110932076839 1106 60-61 0.9842496309099431 1106 62-63 0.9842588856699734 1106 64-65 0.33806490737805106 1106 66-67 0.8279435628465883 1106 68-69 -0.27889739253345525 1106 70-71 -0.20728939536767754 1106 72-73 -0.1881042540981106 1106 74-75 -0.39706482532170284 1106 76-77 -0.7793146995854734 1106 78-79 -0.8070863935530284 1106 80-81 -0.6210180829610543 1106 82-83 -0.27237151597757503 1106 84-85 -0.37979325673106956 1106 86-87 -0.507920140784357 1106 88-89 -0.8967898788650395 1106 90-91 -0.6260031810563191 1106 92-93 0.15333999116646524 1106 94-95 -0.6836950517227471 1106 96-97 -0.8007783969041995 1106 98-99 -0.07735377388114983 1106 100-101 0.7367338076381209 1107 1 0.2659533672416359 1107 2 0.3393482294233223 1107 3 0.3687638870894716 1107 4 0.29562981050238335 1107 5 -0.029423838099148725 1107 6 0.25673432823789 1107 7 0.23221694045304986 1107 8 0.050893449767066556 1107 9 -0.039132397978498545 1107 10-11 0.24259331298340214 1107 12-13 0.31342624306697786 1107 14-15 0.4851911924625796 1107 16-17 0.13036794263621232 1107 18-19 0.01655139193778865 1107 20-21 0.09725737177760863 1107 22-23 0.24730450624119982 1107 24-25 0.4425611068348232 1107 26-27 0.22408685660479222 1107 28-29 0.330982933149663 1107 30-31 0.2746723430058964 1107 32-33 0.3812334669078865 1107 34-35 0.5296031656548692 1107 36-37 0.2860147026365354 1107 38-39 0.3213235945180841 1107 40-41 0.5739655874938947 1107 42-43 0.4643002915100354 1107 44-45 0.6354353298817301 1107 46-47 0.4213677004746188 1107 48-49 0.0456510044712104 1107 50-51 0.05453941979379806 1107 52-53 0.4316024504158342 1107 54-55 0.5510763531829213 1107 56-57 0.6934272236282304 1107 58-59 0.8866228351775653 1107 60-61 1.1926995842619341 1107 62-63 0.536254753989315 1107 64-65 0.3955085965690408 1107 66-67 0.952193862726638 1107 68-69 0.7274667284848029 1107 70-71 0.994063396848766 1107 72-73 1.410689561708903 1107 74-75 1.238497616368285 1107 76-77 1.1995104370265501 1107 78-79 1.260186697210667 1107 80-81 1.3621886343520195 1107 82-83 1.3301041604185322 1107 84-85 1.146296307443265 1107 86-87 1.5946554956277446 1107 88-89 1.889362326919315 1107 90-91 2.0572401882626217 1107 92-93 2.0837844800375827 1107 94-95 2.2809024426128524 1107 96-97 2.4577348644579224 1107 98-99 2.728074054987303 1107 100-101 3.230136446582545 1108 1 0.34456251948390104 1108 2 0.2934954346008354 1108 3 0.33045146531702585 1108 4 -0.0476861219700595 1108 5 -0.013671479824580501 1108 6 0.06584383155015416 1108 7 0.2407342039387217 1108 8 0.1636021837781172 1108 9 0.21669078485265203 1108 10-11 0.16641112291716098 1108 12-13 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-2.6358583460944978 2316 58-59 -2.54593130366073 2316 60-61 -2.7182496163047496 2316 62-63 -2.6046074196597466 2316 64-65 -2.2533985072560974 2316 66-67 -2.5342967262229656 2316 68-69 -2.4009991691067754 2316 70-71 -2.6889967124408507 2316 72-73 -2.1174929924299377 2316 74-75 -2.2119172794018027 2316 76-77 -1.5987591440299163 2316 78-79 -2.2458849482052408 2316 80-81 -2.248163521082102 2316 82-83 -2.89940403630278 2316 84-85 -2.359016382295657 2316 86-87 -2.4341848200978156 2316 88-89 -2.9277293549264662 2316 90-91 -2.8968176947570115 2316 92-93 -2.4581098733321802 2316 94-95 -3.4834165211596613 2316 96-97 -3.7606925793793557 2316 98-99 -3.767018031634837 2316 100-101 -4.4336967855567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 9.0 6 31.0 7 65.0 8 129.0 9 228.0 10 375.0 11 686.0 12 1175.0 13 1665.0 14 1982.0 15 2794.0 16 4026.0 17 6023.0 18 5120.0 19 2570.0 20 2085.0 21 2167.0 22 2337.0 23 2607.0 24 2890.0 25 2991.0 26 3251.0 27 3672.0 28 4278.0 29 5039.0 30 5909.0 31 7052.0 32 8577.0 33 10858.0 34 15514.0 35 25539.0 36 26150.0 37 27077.0 38 17456.0 39 1368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.62715293909645 19.091726132886745 14.35440066399171 12.926720264025107 2 14.268251973451676 26.980815300632287 38.408180497192 20.34275222872403 3 24.10741022557129 35.221521317591616 29.169141650916767 11.501926805920327 4 10.685550182862473 18.33533786603176 36.23622395130213 34.74288799980364 5 10.07399115248462 47.06367559764917 27.824012019266174 15.03832123060003 6 23.028889816155715 37.3716894528853 18.779607766132397 20.819812964826585 7 23.38136030043445 43.16732529883901 17.445816253896567 16.005498146829975 8 27.951694852850938 30.19366240396652 18.886625266930118 22.968017476252424 9 31.68258020176235 19.260204707788223 20.111435654500383 28.945779435949042 10-11 24.18644608625218 30.60283252742937 21.86151542672001 23.34920595959844 12-13 32.361525338439066 29.38154325760644 23.858512218172 14.39841918578249 14-15 27.871431727252645 28.050857858177263 30.11389018433519 13.9638202302349 16-17 16.57543997447289 33.035026140742744 31.31857342726001 19.07096045752436 18-19 16.282860018163518 31.145774526889376 37.93549495594119 14.635870499005915 20-21 19.51326673375715 28.961242973908348 35.78900861539972 15.736481676934783 22-23 17.638987751896124 29.822782945926708 34.52050759676984 18.01772170540733 24-25 17.953903929702268 30.5377874868069 36.1485972361994 15.359711347291427 26-27 19.732798570474806 28.471458586969327 37.40574559164278 14.389997250913089 28-29 16.762476558894857 29.279044879284445 39.54943986804253 14.409038693778168 30-31 15.793672222085858 34.27898186102452 36.95343756903365 12.973908347855968 32-33 14.734051692398321 34.071819542966544 35.722245403892884 15.47188336074225 34-35 15.51115583809921 31.474681524753933 36.083552195576935 16.930610441569918 36-37 17.949731228983087 32.21496772293268 36.09999754547017 13.735303502614075 38-39 19.893227952185757 29.7918558700081 36.684912005105424 13.630004172700719 40-41 15.484646915883262 32.634692324685204 37.853759112442006 14.02690164698952 42-43 14.764718445219621 35.301929728522225 35.30242069112806 14.630931135130092 44-45 15.802999435458137 35.53300115362902 32.554920105053874 16.109079305858963 46-47 15.339093296679021 34.61034338872389 30.762622419675512 19.28794089492158 48-49 17.0084190373334 34.134164600770724 31.094720306325325 17.762696055570558 50-51 18.99020642595911 35.61817333889694 30.19145332711519 15.200166908028766 52-53 15.9198350555951 36.15424265481947 31.350973221079503 16.57494906850593 54-55 13.73407623769667 35.701381900296994 31.80309761665153 18.761444245354802 56-57 16.852065486855995 37.35843499177732 27.557742814363905 18.231756707002774 58-59 16.261014702633712 35.224466753393386 29.066787756805184 19.447730787167718 60-61 16.58034903414251 34.124101028448 30.12321739770747 19.17233253970202 62-63 20.70911366927665 33.584349917773245 29.290149971772905 16.416386441177192 64-65 19.24965022949854 34.27750914312364 30.90007609042488 15.572764536952945 66-67 16.7666478486046 34.52468029748901 31.60624432389976 17.10242753000663 68-69 16.922265040131563 36.68736653494023 30.16617166981665 16.22419675511156 70-71 16.723939029478903 36.794384035737956 27.88002258167448 18.601654353108664 72-73 16.520008149059056 34.73659148423329 27.74754607777442 20.995854288933234 74-75 17.100505879840654 35.49823396144906 28.79538743621275 18.60587272249754 76-77 17.852286394541125 33.37350580496306 31.050784222282225 17.72342357821359 78-79 15.78900861539972 32.62143786357723 33.393387496625024 18.19616602439803 80-81 14.887459807073956 36.811074838614665 31.05520237598488 17.246262978326502 82-83 14.551680125671929 35.97113472914263 29.15760536069316 20.31957978449228 84-85 15.736481676934783 33.27777914140546 28.92859772710537 22.05714145455438 86-87 16.98215556810093 33.27974276527331 29.898873370805823 19.839228295819936 88-89 18.266611030657078 30.50784222282222 31.425100022090767 19.800446724429936 90-91 15.612411968157572 32.02554856510245 32.33115928332445 20.030880183415526 92-93 13.952380952380953 34.50760922925871 31.317623956799213 20.22238586156112 94-95 13.821702952799392 34.74509707665497 29.554748287965438 21.878451682580202 96-97 14.210255025649838 32.76920055963281 28.198866007216317 24.821678407501043 98-99 16.288259983800103 31.848751871579 28.42026459831619 23.442723546304705 100-101 17.486743912018852 32.15362333071485 29.4221327572663 20.9375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3101.0 1 2010.5 2 1187.5 3 1427.5 4 1200.5 5 1216.0 6 2220.0 7 4060.0 8 5112.5 9 4821.0 10 3788.5 11 2960.0 12 2855.0 13 2661.0 14 2301.5 15 1896.0 16 1525.5 17 1343.0 18 1241.5 19 1166.0 20 1093.5 21 1074.5 22 1156.5 23 1197.0 24 1225.0 25 1313.5 26 1431.0 27 1532.0 28 1623.5 29 1771.0 30 2044.5 31 2240.0 32 2495.5 33 2809.0 34 3098.5 35 3318.0 36 3596.0 37 3931.5 38 3916.5 39 3937.0 40 3930.5 41 3833.0 42 3804.0 43 3752.0 44 4122.5 45 4198.0 46 4433.0 47 6641.5 48 19927.0 49 19874.0 50 8670.0 51 6426.5 52 2764.0 53 1680.0 54 1383.5 55 1283.5 56 1475.5 57 1690.0 58 1706.5 59 1880.0 60 2110.0 61 2154.0 62 1806.0 63 895.0 64 328.5 65 263.0 66 261.5 67 200.5 68 128.0 69 114.0 70 105.0 71 91.5 72 74.5 73 84.5 74 81.5 75 50.5 76 39.5 77 31.5 78 24.0 79 15.5 80 9.0 81 6.0 82 4.5 83 3.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0431997250926585 2 4.909059669620284E-4 3 0.0 4 0.0 5 0.015708990942784908 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006136324587025355 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.909059669620284E-4 28-29 0.0014727179008860853 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011536290223607666 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.454529834810142E-4 74-75 2.454529834810142E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007118136520949413 92-93 0.002454529834810142 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.01227264917405071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 203705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.37323580668123 #Duplication Level Percentage of deduplicated Percentage of total 1 77.21650956869466 26.541812915735992 2 11.65952584975721 8.015512628556 3 4.300199942873465 4.434353599568003 4 2.010854041702371 2.764782405930144 5 1.1510996858040559 1.9783510468569747 6 0.6983718937446444 1.4403181070665914 7 0.4998571836618109 1.2027196190569696 8 0.3670379891459583 1.0093026680739305 9 0.23707512139388748 0.7334135146412705 >10 1.5238503284775777 9.817628433273606 >50 0.15424164524421594 3.6641221374045805 >100 0.13853184804341617 9.938391301146265 >500 0.014281633818908884 3.710758204265973 >1k 0.025706940874035987 19.100660268525562 >5k 0.0028563267637817767 5.647873149898137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6080 2.9847082791291326 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5425 2.663164870769004 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4837 2.3745121621953316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4835 2.3735303502614076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4458 2.188458800716723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3986 1.956751184310645 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2698 1.3244642988635527 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1690 0.8296310841658281 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1677 0.8232493065953217 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1646 0.8080312216194988 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1564 0.7677769323286124 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1493 0.7329226086743085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1453 0.7132863699958273 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1407 0.690704695515574 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1292 0.6342505093149408 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1290 0.6332686973810167 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1252 0.6146142706364596 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1188 0.5831962887508898 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1073 0.5267421025502564 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1070 0.5252693846493705 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 964 0.47323335215139534 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.4516334896050661 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 840 0.4123610122481039 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 828 0.40647014064455955 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 789 0.3873248079330404 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 752 0.36916128715544533 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 717 0.3519795783117744 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 639 0.3136889128887362 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.29748901597898925 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 504 0.2474166073488623 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 495 0.24299845364620407 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 468 0.2297439925382293 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 433 0.2125622836945583 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 427 0.20961684789278612 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 404 0.1983260106526595 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 400 0.19636238678481135 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 397 0.19488966888392528 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 380 0.1865442674455708 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 376 0.18458064357772266 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.1762352421393682 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 353 0.17328980633759603 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 346 0.16985346456886183 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 344 0.16887165263493778 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 335 0.1644534989322795 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 330 0.1619989690974694 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 308 0.15119903782430474 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 285 0.1399082005841781 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 285 0.1399082005841781 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 284 0.13941729461721608 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 275 0.1349991409145578 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 272 0.13352642301367174 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 267 0.1310718931788616 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 265 0.13009008124493754 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 262 0.12861736334405144 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 260 0.1276355514101274 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 252 0.12370830367443116 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 251 0.12321739770746913 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 247 0.12125377383962102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 245 0.12027196190569696 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.11192656046734248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 227 0.11143565450038045 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 218 0.1070175007977222 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 218 0.1070175007977222 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 211 0.10358115902898798 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.10259934709506394 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 208 0.1021084411281019 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 208 0.1021084411281019 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 208 0.1021084411281019 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 206 0.10112662919417785 No Hit GAGTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 206 0.10112662919417785 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 206 0.10112662919417785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.001227264917405071 0.0 22-23 0.0 0.0 0.0 0.004172700719177242 0.0 24-25 0.0 0.0 0.0 0.005890871603544341 0.0 26-27 0.0 0.0 0.0 0.008590854421835497 0.0 28-29 0.0 0.0 0.0 0.013254461107974767 0.0 30-31 0.0 0.0 0.0 0.02405439238113939 0.0 32-33 0.0 0.0 0.0 0.03043616995164576 0.0 34-35 0.0 0.0 0.0 0.03853611840651923 0.0 36-37 0.0 0.0 0.0 0.048599690729240815 0.0 38-39 0.0 0.0 0.0 0.05645418620063327 0.0 40-41 0.0 0.0 0.0 0.07584497189563338 0.0 42-43 0.0 0.0 0.0 0.0932721337227854 0.0 44-45 0.0 0.0 0.0 0.1310718931788616 0.0 46-47 0.0 0.0 0.0 0.2228713090007609 0.0 48-49 0.0 0.0 0.0 0.28349819592057146 0.0 50-51 0.0 0.0 0.0 0.35320684322917945 0.0 52-53 0.0 0.0 0.0 0.4388699344640534 0.0 54-55 0.0 0.0 0.0 0.5264966495667754 0.0 56-57 0.0 0.0 0.0 0.6205051422400039 0.0 58-59 0.0 0.0 0.0 0.7756314278000049 0.0 60-61 0.0 0.0 0.0 0.9960482069659558 0.0 62-63 0.0 0.0 0.0 1.1845560982793746 0.0 64-65 0.0 0.0 0.0 1.312928008639945 0.0 66-67 0.0 0.0 0.0 1.4989813701185537 0.0 68-69 0.0 0.0 0.0 1.7019709874573525 0.0 70-71 0.0 0.0 0.0 1.8848334601507082 0.0 72-73 0.0 0.0 0.0 2.2120222871309 0.0 74-75 0.0 0.0 0.0 2.4928205002331802 0.0 76-77 0.0 0.0 0.0 2.7024373481259665 0.0 78-79 0.0 0.0 0.0 2.953781203210525 0.0 80-81 0.0 0.0 0.0 3.211015929898628 0.0 82-83 0.0 0.0 0.0 3.506295869026288 0.0 84-85 0.0 0.0 0.0 4.043592449866228 0.0 86-87 0.0 0.0 0.0 4.74092437593579 0.0 88-89 0.0 0.0 0.0 5.263002871799907 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGT 20 0.0020630509 71.32447 1 GGAGGGC 30 1.2951158E-4 63.368378 8 TAGGGGT 25 0.0050043883 57.03154 7 TAGTAGG 25 0.00501413 57.003532 4 TCCTAGA 35 2.7816332E-4 54.28908 2 GGATATG 75 2.3464963E-9 50.719624 1 GTGTTAG 20 5.3693913E-4 47.526287 10-11 GAGGTGC 40 5.382548E-4 47.491287 6 GATTGGC 75 1.3495264E-7 44.35787 9 ATATGAG 85 3.619316E-7 39.120075 3 AGATTGG 90 5.6428144E-7 36.964886 8 TACCTGG 90 5.6645695E-7 36.94673 2 GTCACTA 65 1.3162315E-4 36.576653 1 GATATGA 95 8.6490763E-7 35.00217 2 TGGGGAC 95 8.66572E-7 34.993576 6 GTGTAAG 55 0.002561504 34.58156 1 CTACCTC 110 6.9007E-8 34.58156 1 GGGATGT 100 1.2866676E-6 33.2684 7 TACATGA 100 1.2916189E-6 33.25206 2 CATGGGT 220 0.0 32.388374 4 >>END_MODULE