FastQCFastQC Report
Thu 26 May 2016
SRR937795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622970
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.4072780149971965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.3283486447685416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.21208032187902426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21250.13093279604675379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.036.8762361
GTATCAA77100.034.1059271
TATCAAC95600.027.2985342
ATCAACG95900.026.964463
TCAACGC96500.026.8969884
CAACGCA97750.026.6016675
AACGCAG100700.026.0423166
GTACATG66550.025.3652631
TACATGG67200.024.4047682
ACGCAGA112200.023.0766967
GAGTACT60700.023.0472812-13
ACATGGG67650.022.9066333
CGCAGAG112800.022.8697138
ATTCGCG953.3018478E-722.4921580-81
GCAGAGT119250.021.114179
GTACTTT66400.021.03337714-15
AGAGTAC97750.020.65357410-11
AGTACTT64400.020.50604812-13
CATGGGA35750.019.6799494
GTATAGG4200.019.2468071
Produced by FastQC (version 0.11.5)