##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937795_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1622970 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.542757413877027 33.0 31.0 34.0 30.0 34.0 2 31.67832923590701 33.0 31.0 34.0 30.0 34.0 3 31.69270842960745 33.0 31.0 34.0 30.0 34.0 4 35.29608310689662 37.0 35.0 37.0 33.0 37.0 5 35.28066014775381 37.0 35.0 37.0 33.0 37.0 6 35.34186830317258 37.0 35.0 37.0 33.0 37.0 7 35.270457248131514 37.0 35.0 37.0 33.0 37.0 8 35.25396957429897 37.0 35.0 37.0 32.0 37.0 9 36.862489756434194 39.0 37.0 39.0 33.0 39.0 10-11 36.72191815005823 39.0 37.0 39.0 32.0 39.0 12-13 36.59465732576696 39.0 37.0 39.0 32.0 39.0 14-15 37.69982994140372 40.0 37.5 41.0 32.0 41.0 16-17 37.602499430057236 40.0 37.0 41.0 32.0 41.0 18-19 37.53462664128111 40.0 37.0 41.0 32.0 41.0 20-21 37.42269173182498 40.0 37.0 41.0 32.0 41.0 22-23 37.331376735244646 40.0 37.0 41.0 32.0 41.0 24-25 37.366029563084965 40.0 37.0 41.0 32.0 41.0 26-27 37.273929893959846 40.0 37.0 41.0 31.5 41.0 28-29 37.14153681214071 39.5 37.0 41.0 31.0 41.0 30-31 36.892338428929676 39.0 36.0 41.0 30.5 41.0 32-33 36.738898131203904 39.0 36.0 41.0 30.0 41.0 34-35 36.724224415731655 39.0 36.0 41.0 30.0 41.0 36-37 36.56828006679113 39.0 36.0 41.0 30.0 41.0 38-39 36.402397456514905 39.0 35.0 41.0 30.0 41.0 40-41 36.168712915211 39.0 35.0 41.0 29.5 41.0 42-43 35.884636807827626 39.0 35.0 40.5 28.5 41.0 44-45 35.63261428122516 39.0 35.0 40.0 27.5 41.0 46-47 35.26765775091344 38.5 35.0 40.0 26.0 41.0 48-49 34.935269290251824 38.0 34.5 40.0 24.5 41.0 50-51 34.03638360536547 37.5 33.5 39.5 23.5 40.5 52-53 34.123208377234334 38.0 33.5 39.5 23.5 40.5 54-55 34.39183657122436 38.0 34.0 40.0 23.0 41.0 56-57 34.14283535740032 38.0 34.0 40.0 22.5 41.0 58-59 33.87164334522511 38.0 33.0 40.0 22.0 41.0 60-61 33.523141832566225 37.5 33.0 40.0 20.5 41.0 62-63 33.12857107648324 37.0 32.5 40.0 18.5 41.0 64-65 32.72087500077019 36.0 32.0 39.0 17.5 41.0 66-67 32.24908901581668 36.0 31.0 39.0 13.0 40.5 68-69 31.723871667375246 35.0 31.0 39.0 8.0 40.0 70-71 31.24951138961287 35.0 30.5 38.0 7.5 40.0 72-73 30.764745497452203 35.0 30.0 37.5 4.5 39.5 74-75 30.21043673019218 34.5 29.0 37.0 2.0 39.0 76-77 29.704837427678886 34.0 29.0 36.0 2.0 39.0 78-79 29.13524002292094 34.0 28.5 36.0 2.0 38.0 80-81 28.55558975212111 34.0 27.5 35.0 2.0 37.0 82-83 28.034199954404578 33.5 26.5 35.0 2.0 37.0 84-85 27.49779632402324 33.0 26.0 35.0 2.0 36.0 86-87 26.94805911384684 33.0 25.5 35.0 2.0 36.0 88-89 26.43449047117322 32.0 24.5 35.0 2.0 35.0 90-91 25.84355594989433 32.0 23.0 34.5 2.0 35.0 92-93 25.178076304552764 32.0 19.5 34.0 2.0 35.0 94-95 24.40491229043051 31.0 14.0 34.0 2.0 35.0 96-97 23.525765109644663 31.0 2.0 34.0 2.0 35.0 98-99 22.426103686451384 30.5 2.0 34.0 2.0 35.0 100-101 19.63725577182573 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10549471401726862 1101 2 0.1759278172625791 1101 3 0.1895302821435827 1101 4 0.02094549281788005 1101 5 -0.01636021250758546 1101 6 0.14891887108446866 1101 7 0.2563610438721895 1101 8 0.16492439476306942 1101 9 0.2578590528652427 1101 10-11 0.22672795305304305 1101 12-13 0.19774301311294096 1101 14-15 0.38702289213335916 1101 16-17 0.3637386645789604 1101 18-19 0.3927537781270587 1101 20-21 0.3779426232436336 1101 22-23 0.368614013567111 1101 24-25 0.41952125979226196 1101 26-27 0.25576799834050234 1101 28-29 0.5253885468080242 1101 30-31 0.42504627218934843 1101 32-33 0.36514538006068165 1101 34-35 0.4074436348307344 1101 36-37 0.4138821488808375 1101 38-39 0.34918627873258856 1101 40-41 0.317792113446977 1101 42-43 0.4647680335558704 1101 44-45 0.685751145608549 1101 46-47 0.4946119993661071 1101 48-49 0.5012197481435265 1101 50-51 0.37378587671726393 1101 52-53 0.29649010803785103 1101 54-55 0.561357318287186 1101 56-57 0.4664820621701793 1101 58-59 0.4312458416831646 1101 60-61 0.5062327605104997 1101 62-63 0.49727223464756065 1101 64-65 0.5262658954096153 1101 66-67 0.6062921623411128 1101 68-69 0.3444593428424163 1101 70-71 0.3664324993271073 1101 72-73 0.32896339890770676 1101 74-75 0.3962909355882509 1101 76-77 0.5012051731834894 1101 78-79 0.4983023529576762 1101 80-81 0.653555988663971 1101 82-83 0.7620903997510062 1101 84-85 0.6386923796361721 1101 86-87 0.743766636697984 1101 88-89 0.8120885025880575 1101 90-91 0.8538080142934952 1101 92-93 0.7654237034616713 1101 94-95 0.927872781991784 1101 96-97 1.366293380251971 1101 98-99 1.5589290290449682 1101 100-101 1.5384954248265217 1102 1 0.22710131899406605 1102 2 0.3642456376745038 1102 3 0.2888009939288807 1102 4 0.19601333358203732 1102 5 0.15543906023942355 1102 6 0.2344715019493009 1102 7 0.24695288669870052 1102 8 0.2088180475811754 1102 9 0.21329241966668633 1102 10-11 0.25629092285033295 1102 12-13 0.33541465024626405 1102 14-15 0.42511005394714374 1102 16-17 0.4964351992727316 1102 18-19 0.3785597942460228 1102 20-21 0.44704013755803373 1102 22-23 0.4229188492567886 1102 24-25 0.4824652775936755 1102 26-27 0.2626551927178582 1102 28-29 0.3306194089284631 1102 30-31 0.3448754491451993 1102 32-33 0.41309285774106996 1102 34-35 0.4863370162857805 1102 36-37 0.5337676336709336 1102 38-39 0.6646207309862575 1102 40-41 0.5068270107360604 1102 42-43 0.3734865893616899 1102 44-45 0.5131476206024459 1102 46-47 0.565776960968229 1102 48-49 0.48639755878637914 1102 50-51 0.5566991626525137 1102 52-53 0.5112895707390024 1102 54-55 0.42459033774391486 1102 56-57 0.36549972790516705 1102 58-59 0.6662116499380275 1102 60-61 0.6488574518350205 1102 62-63 0.5589411852866206 1102 64-65 0.4631132672584144 1102 66-67 0.5224925368221278 1102 68-69 0.5749893827327917 1102 70-71 0.6020597550325171 1102 72-73 0.4532849912661234 1102 74-75 0.5130370890169225 1102 76-77 0.5109233626491765 1102 78-79 0.7068814959931409 1102 80-81 0.6186084974154262 1102 82-83 0.695450417118245 1102 84-85 0.5071603316591826 1102 86-87 0.661188484137675 1102 88-89 0.6825146782685003 1102 90-91 0.9206525368301932 1102 92-93 0.9069704050437153 1102 94-95 1.0439727521418511 1102 96-97 1.1534077055077319 1102 98-99 1.2761972171538432 1102 100-101 1.2487027462017863 1103 1 0.31842418768207637 1103 2 0.39082734385439366 1103 3 0.3873125370637496 1103 4 0.2714583871297549 1103 5 0.26071117869431504 1103 6 0.2888302009800725 1103 7 0.45688535158892307 1103 8 0.4026925566094022 1103 9 0.39298596850954937 1103 10-11 0.4801012876529498 1103 12-13 0.5056536369370406 1103 14-15 0.3472624176759922 1103 16-17 0.5610668644794359 1103 18-19 0.42516855685587274 1103 20-21 0.3319072572845059 1103 22-23 0.29414119235774905 1103 24-25 0.4490947387256625 1103 26-27 0.4369621807649935 1103 28-29 0.38223325786456286 1103 30-31 0.42380887478592655 1103 32-33 0.3557349923026578 1103 34-35 0.4922583477729603 1103 36-37 0.41218041730426336 1103 38-39 0.42541010056437756 1103 40-41 0.5291609280549991 1103 42-43 0.39260093280174146 1103 44-45 0.4975887605046765 1103 46-47 0.6410828469780157 1103 48-49 0.762621456042595 1103 50-51 0.5527512551106284 1103 52-53 0.48078079682620256 1103 54-55 0.48118984606571047 1103 56-57 0.3670383415986862 1103 58-59 0.3149436005441473 1103 60-61 0.5078139778741857 1103 62-63 0.35695221978886593 1103 64-65 0.7005602846932035 1103 66-67 0.5681394892649791 1103 68-69 0.6828763673890386 1103 70-71 0.5855532134517993 1103 72-73 0.3611977607707715 1103 74-75 0.4618407426946156 1103 76-77 0.6804640391305696 1103 78-79 0.8749308898725978 1103 80-81 0.9944286675247831 1103 82-83 1.0174784484274078 1103 84-85 0.8955765768201083 1103 86-87 0.9595767545041731 1103 88-89 1.0484143928690877 1103 90-91 1.0142843857097894 1103 92-93 0.8864947628950262 1103 94-95 0.945485934204072 1103 96-97 1.236595984351439 1103 98-99 1.5715163841266317 1103 100-101 1.5048939355544135 1104 1 0.300003146849555 1104 2 0.3178112430980029 1104 3 0.26706052652420453 1104 4 0.16708444545594858 1104 5 0.3121004349927077 1104 6 0.2976226218325948 1104 7 0.30720346594868175 1104 8 0.37692282746618133 1104 9 0.38061485451893873 1104 10-11 0.40470549420535207 1104 12-13 0.4867980565221899 1104 14-15 0.6371384300448639 1104 16-17 0.6380367897072432 1104 18-19 0.3893098872120717 1104 20-21 0.5788856565946148 1104 22-23 0.5531589161923378 1104 24-25 0.3226366211675753 1104 26-27 0.4668744209566569 1104 28-29 0.53074491309048 1104 30-31 0.7613168519313191 1104 32-33 0.6851197320795777 1104 34-35 0.38609244407260945 1104 36-37 0.5464296091279053 1104 38-39 0.6750207710331679 1104 40-41 0.7098255232793278 1104 42-43 0.5975436991188303 1104 44-45 0.7156065725175509 1104 46-47 0.717497547941548 1104 48-49 0.6839264497729687 1104 50-51 0.7154469100137106 1104 52-53 0.7481048400544879 1104 54-55 0.8994108417629079 1104 56-57 0.92411696524033 1104 58-59 0.7696606902423895 1104 60-61 0.9393330804285185 1104 62-63 1.0262582567131489 1104 64-65 1.0169159360990534 1104 66-67 1.1769062786339717 1104 68-69 0.9875480228540674 1104 70-71 1.0143884829709542 1104 72-73 1.1867620644089492 1104 74-75 1.087691326838371 1104 76-77 0.9359176416617352 1104 78-79 0.930596419188884 1104 80-81 1.0642419537499883 1104 82-83 1.1896865222699908 1104 84-85 1.2138964368109342 1104 86-87 1.3398130872441705 1104 88-89 1.389609417820548 1104 90-91 1.4574788905588498 1104 92-93 1.2197585997861289 1104 94-95 1.1935665620334355 1104 96-97 1.7843310728809385 1104 98-99 1.8006558687171008 1104 100-101 2.0365499386272035 1105 1 0.37230480736615945 1105 2 0.3774189884101524 1105 3 0.31890303474520465 1105 4 0.28295270235280157 1105 5 0.35011158041028523 1105 6 0.3653329162824761 1105 7 0.2757675520997509 1105 8 0.3236643930961236 1105 9 0.26674222982330775 1105 10-11 0.3542946010658028 1105 12-13 0.4215842634403799 1105 14-15 0.45904159167852043 1105 16-17 0.5051338822342899 1105 18-19 0.6288712560400498 1105 20-21 0.5690812505148486 1105 22-23 0.6904645303199146 1105 24-25 0.5692340839534324 1105 26-27 0.6417679715503226 1105 28-29 0.5387203818757698 1105 30-31 0.7551373736824516 1105 32-33 0.5529036076224187 1105 34-35 0.6268626451419692 1105 36-37 0.6811394893899632 1105 38-39 0.7677768771656659 1105 40-41 0.7279278057052139 1105 42-43 0.6285218883650145 1105 44-45 0.674717684674583 1105 46-47 0.555098270887548 1105 48-49 0.4911377106610857 1105 50-51 0.591665940061155 1105 52-53 0.5757799599179307 1105 54-55 0.40679993669977677 1105 56-57 0.40607430075755957 1105 58-59 0.474122779506132 1105 60-61 0.5521604300030347 1105 62-63 0.5375244790692477 1105 64-65 0.3897743968265175 1105 66-67 0.6863051258112094 1105 68-69 0.7126881250204029 1105 70-71 0.6260289648094535 1105 72-73 0.6779601811095013 1105 74-75 0.501170394664765 1105 76-77 0.6499688064080793 1105 78-79 0.7349738666623402 1105 80-81 0.7827028851705293 1105 82-83 0.7068855144129778 1105 84-85 0.6379831840559582 1105 86-87 0.9683654537627326 1105 88-89 1.024628762274201 1105 90-91 0.9319333863793169 1105 92-93 1.0583438032402057 1105 94-95 1.2633293610644891 1105 96-97 1.4049679807806328 1105 98-99 1.3176038379416894 1105 100-101 1.2948450694101439 1106 1 0.30425528092574794 1106 2 0.2638950567380327 1106 3 0.32090438777260744 1106 4 0.14825952950355514 1106 5 0.29787908520225415 1106 6 0.24780275504671323 1106 7 0.23904554285405766 1106 8 0.2718441484236749 1106 9 0.33423504186524866 1106 10-11 0.22558434624564683 1106 12-13 0.04226267983455756 1106 14-15 0.1265738670195944 1106 16-17 0.14611123311939878 1106 18-19 0.3760348314149482 1106 20-21 0.3969596048952724 1106 22-23 0.5616908523404334 1106 24-25 0.5634467206655742 1106 26-27 0.572638489647062 1106 28-29 0.7501062483770724 1106 30-31 0.8169872862994296 1106 32-33 0.7234801946108078 1106 34-35 0.8501051189270683 1106 36-37 0.659244405389515 1106 38-39 0.7128242331246142 1106 40-41 0.7211609069567615 1106 42-43 0.8174154361156027 1106 44-45 0.8222276209695494 1106 46-47 0.8019693950278182 1106 48-49 0.9876564839162398 1106 50-51 0.8252393417975341 1106 52-53 0.9307417650819829 1106 54-55 0.9765671737050781 1106 56-57 0.8858793097777422 1106 58-59 0.9967401828210569 1106 60-61 0.8419249891416101 1106 62-63 1.0270596577816207 1106 64-65 1.1220652413411258 1106 66-67 0.9362716524771244 1106 68-69 0.9457858530618886 1106 70-71 0.9222521156720056 1106 72-73 1.0177409483887878 1106 74-75 0.8054447632519768 1106 76-77 0.8664073846017857 1106 78-79 0.8699823935537587 1106 80-81 0.7772114966261618 1106 82-83 0.8372659124285384 1106 84-85 0.9581609098424053 1106 86-87 1.1475270700667117 1106 88-89 1.3095943023576346 1106 90-91 1.3053020017812784 1106 92-93 1.4052861022592609 1106 94-95 1.4481567796009607 1106 96-97 1.7575091353640104 1106 98-99 1.9697454266213619 1106 100-101 2.1018607278834764 1107 1 0.25984791081443603 1107 2 0.33669150776271906 1107 3 0.36207685599784156 1107 4 0.36705984520994406 1107 5 0.30379912439339307 1107 6 0.35538569601506254 1107 7 0.3549881713819403 1107 8 0.30432326009120203 1107 9 0.4377230531871348 1107 10-11 0.5158137777019789 1107 12-13 0.5249916350096981 1107 14-15 0.45603226725909707 1107 16-17 0.6923774607001576 1107 18-19 0.6959956402765002 1107 20-21 0.6849771864754359 1107 22-23 0.6266898997780359 1107 24-25 0.49703624792246615 1107 26-27 0.4273384324933147 1107 28-29 0.4558218948947754 1107 30-31 0.7069965397626774 1107 32-33 0.7105487518870248 1107 34-35 0.7569945944734258 1107 36-37 0.5780659496292344 1107 38-39 0.6105108673134438 1107 40-41 0.5483707156278044 1107 42-43 0.7959264450152546 1107 44-45 0.7910165059272245 1107 46-47 0.7920159345047253 1107 48-49 0.806904776107956 1107 50-51 0.5831807183862807 1107 52-53 0.6395774616772556 1107 54-55 0.7251677234696601 1107 56-57 0.8698221016020327 1107 58-59 0.8004097253189428 1107 60-61 0.8035868022760795 1107 62-63 0.9396127971551067 1107 64-65 0.7620527219496722 1107 66-67 0.9041926170145942 1107 68-69 0.993070778081627 1107 70-71 0.9821078705096618 1107 72-73 1.2612681508491228 1107 74-75 1.2136365276696885 1107 76-77 1.285017187829613 1107 78-79 1.2594957702513625 1107 80-81 1.5592981525878393 1107 82-83 1.4760999760597038 1107 84-85 1.3478960974971947 1107 86-87 1.5289491096388765 1107 88-89 1.5352933470736367 1107 90-91 1.5670539001020494 1107 92-93 1.6476620650276779 1107 94-95 1.8865308916767312 1107 96-97 1.9764229420754944 1107 98-99 1.9535107696799017 1107 100-101 1.7119505409090863 1108 1 0.03168902565524334 1108 2 0.16036966868225733 1108 3 0.2879897844893513 1108 4 0.19157311954622713 1108 5 0.02553750299170332 1108 6 0.1341833768206726 1108 7 0.200948907827879 1108 8 0.06045590173498283 1108 9 0.26970148874811173 1108 10-11 0.258968085503831 1108 12-13 0.3977568674377281 1108 14-15 0.4343986592843194 1108 16-17 0.49375117649712763 1108 18-19 0.4945344460948249 1108 20-21 0.38685542877021106 1108 22-23 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-1.7732429706193216 2316 100-101 -1.7193771653074137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7283.0 3 1653.0 4 1574.0 5 1755.0 6 1988.0 7 2425.0 8 2855.0 9 3658.0 10 4614.0 11 5702.0 12 7314.0 13 8973.0 14 10324.0 15 12271.0 16 15150.0 17 18693.0 18 16532.0 19 12602.0 20 12993.0 21 13806.0 22 15217.0 23 16508.0 24 18485.0 25 21066.0 26 24086.0 27 27846.0 28 33141.0 29 39162.0 30 46869.0 31 57158.0 32 70979.0 33 90763.0 34 119742.0 35 162899.0 36 225932.0 37 284354.0 38 189116.0 39 17482.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.841485007351245 18.71406649679592 14.732589535953744 22.711858959899093 2 15.203256653614186 19.627961057559833 41.21700685903939 23.951775429786593 3 19.375908187776165 24.182878127378586 29.71429771754495 26.726915967300297 4 12.631873549668974 16.26687573674963 37.4054427417342 33.69580797184721 5 13.597648118661757 37.21031679095233 34.31896834561979 14.873066744766131 6 31.934698261892198 35.94293351606461 17.443614128337856 14.67875409370534 7 28.6384428764561 32.57168608132551 20.55212943928984 18.23774160292856 8 26.195829459489158 35.07350735073507 19.506148145678765 19.224515044097 9 26.220214964019362 17.06161927379423 20.55936820752405 36.15879755466236 10-11 24.98010501588203 26.687678074770567 27.13459751629104 21.197619393056357 12-13 25.882209869787058 23.925234741023267 27.981413937139628 22.21114145205005 14-15 22.71592401511796 25.319589919919256 26.49890195703203 25.465584107930756 16-17 21.89876328660482 28.732310893048137 26.9858178467636 22.383107973583442 18-19 21.810437292137692 27.158966919509542 29.620890017207717 21.409705771145045 20-21 22.61766094758382 26.138862911763162 29.82392138344398 21.41955475720904 22-23 22.307570040816955 26.00779302948234 29.736070924284157 21.94856600541654 24-25 22.078322112581596 26.222423046569126 29.839576120709427 21.859678720139854 26-27 21.87196192745353 26.40131723109875 29.836518905105656 21.89020193634206 28-29 21.746903514222364 26.602908089925027 29.78969485335311 21.860493542499494 30-31 21.90274194051242 26.271196119728344 29.816114314042586 22.00994762571665 32-33 21.7469576595857 26.334476400736033 29.794701637182442 22.12386430249583 34-35 22.19442357402195 26.43440510241187 29.59840916962334 21.772762153942843 36-37 22.11772533799465 26.264239475009628 29.624280310885947 21.99375487610978 38-39 21.84599793488787 26.55039250091498 29.673982611336147 21.929626952861007 40-41 22.084498731448143 26.480776987626797 29.523202879725357 21.911521401199703 42-43 21.95879931913475 26.405318526726123 29.309397082402906 22.326485071736222 44-45 22.000419769929042 26.596900492921012 29.18039921354869 22.222280523601256 46-47 22.191469200164207 26.854672679402057 28.717324271017564 22.236533849416166 48-49 22.241424118830487 26.802047754180602 28.661706342469788 22.294821784519122 50-51 22.244789358079537 27.064594550880383 28.470304187157147 22.220311903882934 52-53 22.361943785560072 27.213812892069644 28.16288424330385 22.261359079066434 54-55 22.21012389367421 27.003315003748323 28.37848139434471 22.40807970823276 56-57 22.278643121099204 27.245801220481248 28.235332237831834 22.24022342058771 58-59 22.425245427426216 26.90204905246637 28.374567321623257 22.298138198484153 60-61 22.332721744244672 26.96481480452909 28.643321366923214 22.059142084303023 62-63 22.153780162843482 27.038158822540105 28.61055350428247 22.197507510333942 64-65 22.115325866051805 27.103559620888063 28.470394300360486 22.310720212699646 66-67 22.13787427750436 27.142507568634578 28.232526722039868 22.48709143182119 68-69 22.07695616938727 26.99844892724154 28.455395702547083 22.46919920082411 70-71 22.221711330280325 27.00295014380408 28.315486403416735 22.45985212249886 72-73 22.272668221704972 27.023784433303327 28.23067231286079 22.472875032130908 74-75 22.04257141834368 27.32332577479167 28.110430417184595 22.523672389680062 76-77 22.147446995381404 27.15908452655832 28.123663350386902 22.569805127673376 78-79 22.250659355377888 27.04492751565757 28.15476781987763 22.549645309086912 80-81 22.162235889985443 27.330366144296974 27.96374569402198 22.543652271695603 82-83 22.230420224234386 27.32542190137896 28.014671234918424 22.429486639468227 84-85 22.250689341071396 27.134920772062 27.92025737045695 22.694132516409653 86-87 22.052418656847898 27.200297204237504 28.075988261173162 22.671295877741432 88-89 22.29051639466427 27.031532200107446 27.9417005917514 22.736250813476886 90-91 22.264359040667156 27.011771632913668 27.980510427088152 22.743358899331028 92-93 22.32842423314852 26.85121871744615 28.101718596487764 22.718638452917567 94-95 22.188031149671726 26.94191714404563 28.04254258146301 22.827509124819635 96-97 22.087606255394352 26.927146608143595 28.109869011210375 22.875378125251686 98-99 22.152312986279185 26.920051559133 27.867945914136204 23.059689540451608 100-101 22.504833862304572 26.188287845508928 28.3898201806308 22.9170581115557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2028.0 1 1174.0 2 331.5 3 357.0 4 319.5 5 297.0 6 481.0 7 998.5 8 1633.0 9 1960.5 10 1841.5 11 1639.0 12 1759.5 13 1966.0 14 2060.0 15 2188.0 16 2289.5 17 2445.5 18 2642.5 19 2813.5 20 3085.0 21 3564.0 22 4356.0 23 5312.5 24 6435.0 25 7862.5 26 9764.0 27 11919.5 28 13894.0 29 16117.0 30 18683.5 31 21608.0 32 24648.0 33 27657.5 34 31049.5 35 34232.5 36 38410.5 37 42545.0 38 44845.0 39 46521.5 40 48393.0 41 51028.5 42 53288.0 43 55621.5 44 58362.5 45 60477.0 46 61475.5 47 63013.5 48 65480.5 49 64680.5 50 61811.5 51 58442.0 52 55375.0 53 53741.0 54 52141.5 55 48574.5 56 43918.5 57 39977.5 58 35936.5 59 31512.5 60 26573.0 61 21745.5 62 17126.0 63 12920.5 64 9793.0 65 7658.5 66 5670.0 67 4180.0 68 3122.5 69 2209.5 70 1591.0 71 1114.0 72 899.5 73 715.0 74 498.5 75 377.0 76 267.0 77 163.5 78 110.0 79 75.5 80 54.0 81 37.5 82 23.5 83 14.5 84 11.5 85 12.0 86 10.5 87 6.0 88 2.0 89 3.0 90 4.0 91 2.0 92 0.5 93 1.5 94 1.5 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3004984688564792 2 0.2696291367061622 3 0.2687665206380894 4 0.2695059058392946 5 0.2785633745540583 6 0.19131592081184495 7 0.18854322630732545 8 0.19297953751455665 9 0.1892209960750969 10-11 0.19809361848955928 12-13 0.18771141795596963 14-15 0.18746495622223455 16-17 0.19424265389994885 18-19 0.18838918772374105 20-21 0.18207360579677997 22-23 0.18068725854452022 24-25 0.18364479934934103 26-27 0.17899283412509165 28-29 0.1915931902622969 30-31 0.19633757863669693 32-33 0.19750827187193848 34-35 0.19636838635341383 36-37 0.1741252148838241 38-39 0.1675323635064111 40-41 0.17347825283276955 42-43 0.1831518758818709 44-45 0.1870028404714813 46-47 0.18946745780883195 48-49 0.18786545653955403 50-51 0.19174722884588133 52-53 0.21192628329543983 54-55 0.17939333444241112 56-57 0.16682378602192277 58-59 0.16540663105294615 60-61 0.16026174236122664 62-63 0.16651570885475392 64-65 0.16371220663351757 66-67 0.16214101308095652 68-69 0.17258482904797995 70-71 0.1669778246055072 72-73 0.16300362914902924 74-75 0.168086902407315 76-77 0.1713525203793046 78-79 0.17418683031725787 80-81 0.16596116995385005 82-83 0.16368139891680067 84-85 0.15909104912598507 86-87 0.15622593147131492 88-89 0.16084708897884742 90-91 0.1675323635064111 92-93 0.15884458739225002 94-95 0.16414351466755392 96-97 0.16269555198186042 98-99 0.16602278538728382 100-101 0.14612100038817724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1622970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.473294037576764 #Duplication Level Percentage of deduplicated Percentage of total 1 78.11110687345268 47.23635933558888 2 12.674844949237063 15.329792509918153 3 4.069076332937486 7.382113486292198 4 1.7513004401693961 4.23627625866006 5 0.9562626126991082 2.891417507744728 6 0.5690775959888893 2.064839807546005 7 0.3752665189662823 1.5885521780734109 8 0.2529964590303152 1.2239623405924798 9 0.18221836689146348 0.9917410392067064 >10 0.9736367240092377 10.447830962840404 >50 0.05838935865224335 2.432729579177188 >100 0.024292887772162302 2.5231750571203677 >500 8.164694366337937E-4 0.3274770100542635 >1k 5.102933978961211E-4 0.5868787954402548 >5k 2.0411735915844842E-4 0.7368541317447893 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6610 0.4072780149971965 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5329 0.3283486447685416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3442 0.21208032187902426 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2125 0.13093279604675379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.161543343376648E-5 14-15 0.0 0.0 0.0 0.0 6.161543343376648E-5 16-17 0.0 0.0 0.0 0.0 6.161543343376648E-5 18-19 0.0 0.0 0.0 0.0 6.161543343376648E-5 20-21 0.0 0.0 0.0 3.080771671688324E-5 1.2323086686753297E-4 22-23 0.0 0.0 0.0 1.8484630030129946E-4 1.2323086686753297E-4 24-25 0.0 0.0 0.0 2.156540170181827E-4 1.2323086686753297E-4 26-27 0.0 0.0 0.0 4.6211575075324866E-4 1.2323086686753297E-4 28-29 0.0 0.0 0.0 0.0011706932352415632 1.2323086686753297E-4 30-31 0.0 0.0 0.0 0.0033272334054233904 1.2323086686753297E-4 32-33 0.0 0.0 0.0 0.006623659094129897 1.2323086686753297E-4 34-35 0.0 0.0 0.0 0.00958119989895069 1.2323086686753297E-4 36-37 0.0 0.0 0.0 0.013709433939013044 1.2323086686753297E-4 38-39 0.0 0.0 0.0 0.01768362939549098 1.2323086686753297E-4 40-41 0.0 0.0 0.0 0.021072478234348137 1.2323086686753297E-4 42-43 0.0 0.0 0.0 0.025077481407542963 1.2323086686753297E-4 44-45 0.0 0.0 0.0 0.029082484580737783 1.2323086686753297E-4 46-47 0.0 0.0 0.0 0.03416575783902352 1.2323086686753297E-4 48-49 0.0 0.0 0.0 0.04041972433255082 1.2323086686753297E-4 50-51 0.0 0.0 0.0 0.04590349790815604 1.2323086686753297E-4 52-53 0.0 0.0 0.0 0.05616246757487815 1.2323086686753297E-4 54-55 0.0 0.0 0.0 0.07692686864205747 1.2323086686753297E-4 56-57 0.0 0.0 0.0 0.11143151136496669 1.2323086686753297E-4 58-59 0.0 0.0 0.0 0.14938661836016687 1.2323086686753297E-4 60-61 0.0 0.0 0.0 0.18518518518518517 1.540385835844162E-4 62-63 0.0 0.0 0.0 0.22440340856577756 1.8484630030129946E-4 64-65 0.0 0.0 0.0 0.27606794949999075 1.8484630030129946E-4 66-67 0.0 0.0 0.0 0.3508382779718664 1.8484630030129946E-4 68-69 0.0 0.0 6.161543343376648E-5 0.4451098911255291 1.8484630030129946E-4 70-71 0.0 0.0 6.161543343376648E-5 0.532727037468345 1.8484630030129946E-4 72-73 0.0 0.0 6.161543343376648E-5 0.6298637682766779 2.156540170181827E-4 74-75 0.0 0.0 6.161543343376648E-5 0.7376599690690524 2.4646173373506593E-4 76-77 0.0 0.0 6.161543343376648E-5 0.8598125658514945 2.4646173373506593E-4 78-79 0.0 0.0 6.161543343376648E-5 0.9743556566048663 2.4646173373506593E-4 80-81 0.0 0.0 6.161543343376648E-5 1.100574871993937 2.4646173373506593E-4 82-83 0.0 0.0 6.161543343376648E-5 1.240719175339039 2.4646173373506593E-4 84-85 0.0 0.0 6.161543343376648E-5 1.4198352403309982 2.4646173373506593E-4 86-87 0.0 0.0 6.161543343376648E-5 1.6314226387425523 2.772694504519492E-4 88-89 0.0 0.0 6.161543343376648E-5 1.8823822991182833 3.0807716716883246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5145 0.0 36.876236 1 GTATCAA 7710 0.0 34.105927 1 TATCAAC 9560 0.0 27.298534 2 ATCAACG 9590 0.0 26.96446 3 TCAACGC 9650 0.0 26.896988 4 CAACGCA 9775 0.0 26.601667 5 AACGCAG 10070 0.0 26.042316 6 GTACATG 6655 0.0 25.365263 1 TACATGG 6720 0.0 24.404768 2 ACGCAGA 11220 0.0 23.076696 7 GAGTACT 6070 0.0 23.04728 12-13 ACATGGG 6765 0.0 22.906633 3 CGCAGAG 11280 0.0 22.869713 8 ATTCGCG 95 3.3018478E-7 22.49215 80-81 GCAGAGT 11925 0.0 21.11417 9 GTACTTT 6640 0.0 21.033377 14-15 AGAGTAC 9775 0.0 20.653574 10-11 AGTACTT 6440 0.0 20.506048 12-13 CATGGGA 3575 0.0 19.679949 4 GTATAGG 420 0.0 19.246807 1 >>END_MODULE