FastQCFastQC Report
Thu 26 May 2016
SRR937795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622970
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.46642883109361233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66110.40733963043063026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.2624817464278452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18420.11349562838499788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.10653308440698227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.033.904321
GTATCAA92000.030.6894781
TATCAAC112300.025.0102652
ATCAACG112000.024.9923933
TCAACGC113450.024.7148554
GTACATG66250.024.6092931
CAACGCA114550.024.4360375
TACATGG66650.024.3857732
AACGCAG116950.023.9212976
ACATGGG66800.023.4772953
GAGTACT71750.022.87367412-13
CGTACAC1302.9323093E-421.934033
GTACTTT74950.021.7049414-15
ACGCAGA131000.021.2851457
AGTACTT73250.021.17314312-13
CGCAGAG132300.021.0041858
AGAGTAC108500.020.24845110-11
CATGGGA35950.020.0934854
GCAGAGT138050.019.9228749
GGGCGTA2953.0177034E-919.3227549
Produced by FastQC (version 0.11.5)