##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937795_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1622970 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10415965791111 33.0 31.0 34.0 31.0 34.0 2 32.38453021312778 34.0 31.0 34.0 31.0 34.0 3 32.47677714313881 34.0 31.0 34.0 31.0 34.0 4 36.12180138881187 37.0 35.0 37.0 35.0 37.0 5 36.028017769891 37.0 35.0 37.0 35.0 37.0 6 36.06833151567805 37.0 35.0 37.0 35.0 37.0 7 36.01294909949044 37.0 35.0 37.0 35.0 37.0 8 35.99025182227644 37.0 35.0 37.0 35.0 37.0 9 37.678091400333955 39.0 37.0 39.0 35.0 39.0 10-11 37.58939351928871 39.0 37.0 39.0 35.0 39.0 12-13 37.48361399163262 39.0 37.0 39.0 35.0 39.0 14-15 38.69526916702095 40.0 38.0 41.0 34.0 41.0 16-17 38.65412022403372 40.0 38.0 41.0 34.0 41.0 18-19 38.62781628742368 40.0 38.0 41.0 34.0 41.0 20-21 38.52092398503977 40.0 38.0 41.0 34.0 41.0 22-23 38.46232154630091 40.0 38.0 41.0 34.0 41.0 24-25 38.48796866239056 40.0 38.0 41.0 34.0 41.0 26-27 38.3529070161494 40.0 38.0 41.0 34.0 41.0 28-29 38.19723531550183 40.0 38.0 41.0 34.0 41.0 30-31 38.24313326802097 40.0 38.0 41.0 34.0 41.0 32-33 38.16536812140705 40.0 38.0 41.0 34.0 41.0 34-35 38.022343912703256 40.0 37.0 41.0 33.0 41.0 36-37 37.87509504180607 40.0 37.0 41.0 33.0 41.0 38-39 37.734281902931045 40.0 37.0 41.0 33.0 41.0 40-41 37.55374868297011 40.0 37.0 41.0 33.0 41.0 42-43 37.33100426994954 40.0 37.0 41.0 32.5 41.0 44-45 37.02834926092288 40.0 36.0 41.0 31.5 41.0 46-47 36.668191032489815 39.5 36.0 41.0 31.0 41.0 48-49 36.33826934570571 39.0 36.0 41.0 30.0 41.0 50-51 36.071453877767304 39.0 35.0 40.5 29.5 41.0 52-53 35.82729532893399 39.0 35.0 40.0 29.0 41.0 54-55 35.58994713395811 39.0 35.0 40.0 28.0 41.0 56-57 35.29796669069668 38.0 35.0 40.0 28.0 41.0 58-59 35.03217773587929 38.0 34.0 40.0 27.0 41.0 60-61 34.689266283418675 38.0 34.0 40.0 26.0 41.0 62-63 34.30884797624109 37.0 34.0 40.0 26.0 41.0 64-65 33.894637916905424 37.0 33.0 39.5 25.5 41.0 66-67 33.452111560903774 36.0 33.0 39.0 24.5 40.0 68-69 32.98350739693278 35.5 32.0 39.0 23.0 40.0 70-71 32.586632531716546 35.0 32.0 38.0 22.5 40.0 72-73 32.08692458887102 35.0 31.0 37.5 21.5 39.5 74-75 31.602565050493848 35.0 31.0 37.0 20.0 39.0 76-77 30.393266049280022 33.5 29.5 35.5 19.5 38.0 78-79 30.984189479780895 34.0 31.0 36.0 20.0 38.0 80-81 30.799903879923846 34.0 31.0 35.5 18.5 37.0 82-83 30.420279487606056 34.0 30.5 35.0 17.5 37.0 84-85 30.026810415472866 34.0 30.0 35.0 13.0 36.5 86-87 29.639499805911385 34.0 30.0 35.0 7.5 36.0 88-89 29.19839060487871 34.0 29.0 35.0 4.5 36.0 90-91 28.63563651823509 34.0 29.0 35.0 2.0 35.0 92-93 28.16154580799399 33.0 28.5 35.0 2.0 35.0 94-95 27.65918716920214 33.0 27.0 35.0 2.0 35.0 96-97 27.048818524063908 33.0 26.0 34.5 2.0 35.0 98-99 26.095661041177593 32.0 24.5 34.0 2.0 35.0 100-101 23.26477753747759 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2629756626577162 1101 2 0.24094578881612705 1101 3 0.251713581499466 1101 4 0.14563465586994795 1101 5 0.005280785577738811 1101 6 0.06044145986603411 1101 7 0.11293864462064107 1101 8 0.1603324093697438 1101 9 0.10589433175127994 1101 10-11 0.15356313953524392 1101 12-13 0.19398080648873162 1101 14-15 0.20605394488287487 1101 16-17 0.2475027193945678 1101 18-19 0.28941342374502455 1101 20-21 0.35139849187991246 1101 22-23 0.24987657991744072 1101 24-25 0.12467296999292898 1101 26-27 0.13246295732998448 1101 28-29 0.005319912925308756 1101 30-31 0.1357390211921583 1101 32-33 0.11701180044617843 1101 34-35 0.07504151080448196 1101 36-37 0.13402452962117195 1101 38-39 0.1193665482687507 1101 40-41 0.17347795727003756 1101 42-43 0.25517963965435797 1101 44-45 0.12704458364446936 1101 46-47 0.05074595834140183 1101 48-49 -0.03653066779897074 1101 50-51 -0.02456460157880258 1101 52-53 0.07327506356431712 1101 54-55 -0.0023964657411355006 1101 56-57 0.06921608921979328 1101 58-59 0.04174298540608845 1101 60-61 0.12523174142592808 1101 62-63 -0.08801244783515472 1101 64-65 -0.06068188787649831 1101 66-67 0.004705888231484323 1101 68-69 0.009007127732758136 1101 70-71 -0.0545154256624798 1101 72-73 -0.09334830216893408 1101 74-75 -0.26313695885741595 1101 76-77 -0.34347449642410055 1101 78-79 -0.19541528233128602 1101 80-81 -0.02580990155997398 1101 82-83 -0.06480022689521903 1101 84-85 -0.15088540887447977 1101 86-87 -0.14096603540849273 1101 88-89 -0.327679798684958 1101 90-91 -0.48016710245448024 1101 92-93 -0.4701736244136008 1101 94-95 -0.3568327456516798 1101 96-97 -0.284429450192242 1101 98-99 -0.4945003399342447 1101 100-101 -0.32799227630086847 1102 1 0.3012141093755787 1102 2 0.2661119986883165 1102 3 0.16429397280499103 1102 4 0.09817054363418976 1102 5 0.054548783732471406 1102 6 0.13264981242092233 1102 7 0.17410048554761914 1102 8 0.19874656366678067 1102 9 0.18106393366167595 1102 10-11 0.20646909866796648 1102 12-13 0.20207081761461865 1102 14-15 0.25697725768306867 1102 16-17 0.2577039351010555 1102 18-19 0.1896627385533094 1102 20-21 0.30037748837933265 1102 22-23 0.2546111868480594 1102 24-25 0.1763476192561768 1102 26-27 0.2607074418758586 1102 28-29 0.1473269140921616 1102 30-31 0.079172374425454 1102 32-33 0.14075437188250817 1102 34-35 0.16423549468551357 1102 36-37 0.1976506863061971 1102 38-39 0.12573204716972697 1102 40-41 0.17793814830962873 1102 42-43 0.23745183036831463 1102 44-45 0.13929696485528353 1102 46-47 0.021761670262371524 1102 48-49 0.037717995737970966 1102 50-51 -0.00898395247836703 1102 52-53 -0.041786942894120216 1102 54-55 -0.04458460174830492 1102 56-57 -0.0027361644503969274 1102 58-59 -0.1004431153781411 1102 60-61 -0.03375275017538826 1102 62-63 -0.08970066525123599 1102 64-65 -0.019197939001301734 1102 66-67 0.05479950848602044 1102 68-69 -0.038431544216436464 1102 70-71 -0.21222417169379781 1102 72-73 -0.1320233042041572 1102 74-75 -0.29428570651690933 1102 76-77 -0.3564432761044287 1102 78-79 -0.20817070280834216 1102 80-81 -0.2396751969386628 1102 82-83 -0.28029912787483724 1102 84-85 -0.22141979057375494 1102 86-87 -0.42443391959074006 1102 88-89 -0.3478726021366967 1102 90-91 -0.41067475489267835 1102 92-93 -0.42525048799939213 1102 94-95 -0.5439253618629074 1102 96-97 -0.6048149214141922 1102 98-99 -0.557325975602204 1102 100-101 -0.6721617018252815 1103 1 0.2965551758086491 1103 2 0.29082024136259577 1103 3 0.2058040465567217 1103 4 0.1350435832792769 1103 5 0.07085540443382143 1103 6 0.1352198200504091 1103 7 0.14518966580137516 1103 8 0.1418107926644936 1103 9 0.2999165432296209 1103 10-11 0.3005982772262783 1103 12-13 0.2506628942485989 1103 14-15 0.31379081556828936 1103 16-17 0.3342651546890636 1103 18-19 0.37546447072997324 1103 20-21 0.19773471802695752 1103 22-23 0.10733307783112878 1103 24-25 0.20548838333541397 1103 26-27 0.2166595032508596 1103 28-29 0.2715067439828118 1103 30-31 0.2964351538201768 1103 32-33 0.2788251707183065 1103 34-35 0.30416986694807946 1103 36-37 0.3112506043598344 1103 38-39 0.38349081401818097 1103 40-41 0.4031596154321804 1103 42-43 0.34969610118693595 1103 44-45 0.3390698462591146 1103 46-47 0.34074214442101436 1103 48-49 0.24282092320130744 1103 50-51 0.21892337748292334 1103 52-53 0.26754929152900075 1103 54-55 0.03246829604480439 1103 56-57 0.11513909339809203 1103 58-59 0.02524707083790645 1103 60-61 0.19184030133779828 1103 62-63 0.13702338742049847 1103 64-65 0.4024648658250527 1103 66-67 0.3915072425276662 1103 68-69 0.44222169549081514 1103 70-71 0.4369401166443865 1103 72-73 0.3955569467212712 1103 74-75 0.3228449696819098 1103 76-77 0.37096743061234605 1103 78-79 0.4272937070365259 1103 80-81 0.43527605910135136 1103 82-83 0.4579571099391728 1103 84-85 0.5895663209298192 1103 86-87 0.34459529852788506 1103 88-89 0.3225581814401224 1103 90-91 0.2431191499930243 1103 92-93 0.4289545615922883 1103 94-95 0.23028272539584194 1103 96-97 0.3821647897955067 1103 98-99 0.7511544137843273 1103 100-101 0.5034228724774721 1104 1 0.2793992691341529 1104 2 0.16005633348050452 1104 3 0.20495589105208722 1104 4 0.11121153450920929 1104 5 0.09494825168064125 1104 6 0.12179280850204321 1104 7 0.1257045074017924 1104 8 0.18592010982084872 1104 9 0.1705037059663752 1104 10-11 0.22122960383115498 1104 12-13 0.24036210169025196 1104 14-15 0.16200386027467317 1104 16-17 0.20998160322657355 1104 18-19 0.10934615341071208 1104 20-21 0.11559889059335404 1104 22-23 0.22398173520470266 1104 24-25 0.10473909212439025 1104 26-27 0.2682680843427434 1104 28-29 0.1820132609556424 1104 30-31 0.21086485704795166 1104 32-33 0.2282586626230909 1104 34-35 0.2755948728141746 1104 36-37 0.11798772397890644 1104 38-39 0.03946885314244497 1104 40-41 -0.023948704861226133 1104 42-43 0.11355204776584316 1104 44-45 -0.03988042866283337 1104 46-47 -0.1344670548155733 1104 48-49 -0.17626803075020092 1104 50-51 -0.1555903342398608 1104 52-53 -0.03355301171760061 1104 54-55 -0.24854280993503153 1104 56-57 -0.09030696166999519 1104 58-59 -0.015628046297223364 1104 60-61 -0.22215523594442743 1104 62-63 -0.3096679841104759 1104 64-65 -0.17937915127853898 1104 66-67 -0.07884990940350889 1104 68-69 0.06582522800244561 1104 70-71 -0.17149002598024055 1104 72-73 -0.05441346124526092 1104 74-75 -0.1730719275186523 1104 76-77 -0.24538568006335382 1104 78-79 -0.2569507022362423 1104 80-81 -0.12752668010465484 1104 82-83 -0.05947902432496477 1104 84-85 -0.1407291958771104 1104 86-87 -0.222792711318192 1104 88-89 -0.1996167881832065 1104 90-91 -0.24875686536494257 1104 92-93 -0.2425768560305137 1104 94-95 -0.2323618159948886 1104 96-97 -0.016948420656159868 1104 98-99 -0.13827973572119845 1104 100-101 -0.3063149241714562 1105 1 0.33967222518003837 1105 2 0.18251268687056665 1105 3 0.13700398704325067 1105 4 0.06072690338739051 1105 5 0.010576311022461482 1105 6 0.03270320587132147 1105 7 0.11389937021776575 1105 8 0.1842947364242562 1105 9 0.17003225354776674 1105 10-11 0.19600101608609322 1105 12-13 0.18531120679354984 1105 14-15 0.15260344420418903 1105 16-17 0.20650258246697462 1105 18-19 0.3184729545001517 1105 20-21 0.129395345522191 1105 22-23 0.13444774356376854 1105 24-25 0.09379096287268851 1105 26-27 0.06361145231723953 1105 28-29 0.036485077357468754 1105 30-31 -0.019977779822291097 1105 32-33 0.10263738090006314 1105 34-35 0.2151995430955722 1105 36-37 0.16083145279884548 1105 38-39 0.1065578658692985 1105 40-41 0.21227013691902386 1105 42-43 0.045018868811602886 1105 44-45 -0.11368861891310189 1105 46-47 -0.12468618213439697 1105 48-49 -0.24436701850028442 1105 50-51 -0.24401747170000476 1105 52-53 -0.2372722300322323 1105 54-55 -0.3074257265703224 1105 56-57 -0.28987724181156693 1105 58-59 -0.1510957019941941 1105 60-61 -0.34573068088255354 1105 62-63 -0.4358073928259856 1105 64-65 -0.2579022579381771 1105 66-67 -0.2573593666919578 1105 68-69 -0.2816179466844062 1105 70-71 -0.3154826553198049 1105 72-73 -0.30635143661991293 1105 74-75 -0.4248000634224596 1105 76-77 -0.5035807939022483 1105 78-79 -0.301799084393533 1105 80-81 -0.38424389651151714 1105 82-83 -0.4015259907129938 1105 84-85 -0.2274591830749415 1105 86-87 -0.44034368465955964 1105 88-89 -0.3775504616525964 1105 90-91 -0.35090671977165044 1105 92-93 -0.23038812444932333 1105 94-95 -0.37100177382304267 1105 96-97 -0.3925077310962024 1105 98-99 -0.28172678243656435 1105 100-101 -0.5348559946586917 1106 1 0.18178027005544806 1106 2 0.13337030892490986 1106 3 0.12460123588753902 1106 4 0.14409664631218533 1106 5 0.1025962681765904 1106 6 0.10836703493886546 1106 7 0.00616808709678196 1106 8 -0.04873604494906658 1106 9 0.042569320860877724 1106 10-11 -0.011093216634613157 1106 12-13 -0.08988188149148613 1106 14-15 0.1178035090367544 1106 16-17 0.057933831762277066 1106 18-19 0.07714254802817067 1106 20-21 0.4106576778031652 1106 22-23 0.22071909602740902 1106 24-25 0.1390063902038463 1106 26-27 0.09734200254838754 1106 28-29 0.2160325178806488 1106 30-31 0.36577068392784895 1106 32-33 0.2854229260573504 1106 34-35 -0.1943484611890085 1106 36-37 0.1515417217130377 1106 38-39 0.24116999801497485 1106 40-41 0.020337562784398244 1106 42-43 -0.22024144802231405 1106 44-45 -0.11567059264182689 1106 46-47 -0.16883046277482805 1106 48-49 -0.20028263751031972 1106 50-51 -0.17413395890262962 1106 52-53 -0.017651923346022613 1106 54-55 -0.08804149497712643 1106 56-57 -0.09833577715940578 1106 58-59 -0.21893029439468137 1106 60-61 0.12643359919412234 1106 62-63 -0.047303545154321114 1106 64-65 -0.39059760126887966 1106 66-67 -0.38834580822990006 1106 68-69 -0.07028140930702875 1106 70-71 -0.15648960367925469 1106 72-73 0.010096655047696856 1106 74-75 -0.015977901961193197 1106 76-77 -0.032646218414594586 1106 78-79 -0.20095626107636022 1106 80-81 -0.4510672418717583 1106 82-83 -0.31556646878267713 1106 84-85 -0.34877359281674103 1106 86-87 -0.045997859312844724 1106 88-89 -0.1747549097301686 1106 90-91 -0.20984304291969735 1106 92-93 -0.23592003334547584 1106 94-95 -0.132643447066922 1106 96-97 -0.2461940260561768 1106 98-99 -0.22794394333407553 1106 100-101 -0.6076922078732601 1107 1 0.2782269401694819 1107 2 0.299167331487034 1107 3 0.2233797787392362 1107 4 0.13052018276810884 1107 5 0.05727933706045718 1107 6 0.11028413479461818 1107 7 0.20622959541129404 1107 8 0.22158537681833934 1107 9 0.15221469549005917 1107 10-11 0.16844383804246377 1107 12-13 0.2162207775183873 1107 14-15 0.32475993611129894 1107 16-17 0.1733454747963279 1107 18-19 0.24881470326862853 1107 20-21 0.29296369527587984 1107 22-23 0.23103861267003367 1107 24-25 0.26450920706692216 1107 26-27 0.21829565358395797 1107 28-29 0.2707751899542359 1107 30-31 0.31847269519530386 1107 32-33 0.2164340029971754 1107 34-35 0.2166272348619387 1107 36-37 0.24386760691566423 1107 38-39 0.18195899183692887 1107 40-41 0.19482467597602948 1107 42-43 0.1876904029424935 1107 44-45 0.11514099051687054 1107 46-47 0.19076728373470786 1107 48-49 0.15958046566579753 1107 50-51 0.16050883020005813 1107 52-53 0.3259596489262506 1107 54-55 0.14987810875691565 1107 56-57 0.08945509457126377 1107 58-59 0.059773404269009234 1107 60-61 0.12967748238275334 1107 62-63 0.08182499633657869 1107 64-65 0.26862483565788864 1107 66-67 0.2325781772911455 1107 68-69 0.25670277815726195 1107 70-71 -0.10217204659355872 1107 72-73 0.1948752523315278 1107 74-75 0.306061125880845 1107 76-77 0.21892360440880054 1107 78-79 0.29998487056776213 1107 80-81 0.2833801358600532 1107 82-83 0.18241137733767587 1107 84-85 0.3770745959539106 1107 86-87 0.3903094645311498 1107 88-89 0.39072803940300105 1107 90-91 0.297639198595693 1107 92-93 0.29721743501584186 1107 94-95 0.18431842322129555 1107 96-97 0.3067929374470779 1107 98-99 0.7914611134114296 1107 100-101 0.6035540158972523 1108 1 0.2556371731061162 1108 2 0.12708819406459781 1108 3 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-0.09741999707740945 2316 100-101 0.20413126207694887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 16.0 5 40.0 6 76.0 7 159.0 8 414.0 9 797.0 10 1413.0 11 2170.0 12 3374.0 13 4794.0 14 6398.0 15 8797.0 16 11786.0 17 16352.0 18 14653.0 19 9219.0 20 8103.0 21 8076.0 22 8585.0 23 9438.0 24 11141.0 25 13060.0 26 15871.0 27 19996.0 28 25066.0 29 31282.0 30 39140.0 31 49203.0 32 62789.0 33 82621.0 34 113698.0 35 162559.0 36 249199.0 37 360661.0 38 253586.0 39 18432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69602819475779 19.7060724847832 15.24650384880136 22.35139547165765 2 15.680080346525196 20.425269721559854 40.04910750044671 23.845542431468232 3 19.517859233380776 24.418750808702562 29.950214729785518 26.11317522813114 4 13.228463865629061 16.989161845259 36.77997744875136 33.002396840360575 5 13.67419533191114 37.50897602715029 34.05755875483935 14.759269886099224 6 32.012236825079945 36.57843336598952 16.881088375016173 14.528241433914365 7 28.616117365077603 33.00369076446268 20.17813021805702 18.202061652402694 8 26.457482270159026 35.563812023635684 18.972131339457906 19.006574366747383 9 26.103131912481437 17.11855425546991 20.273079600978452 36.5052342310702 10-11 25.141838727764533 27.043321811247285 26.663277817827808 21.15156164316038 12-13 26.010280260962364 24.185509293148876 27.708180830061448 22.096029615827316 14-15 22.722539541704407 25.62062145326161 26.303690148308352 25.353148856725632 16-17 21.82628144697684 29.12185684270196 26.876528832942075 22.175332877379127 18-19 21.71555851309636 27.4232733815166 29.592598754135935 21.268569351251102 20-21 22.52848173410476 26.327596936480653 29.936936603880536 21.20698472553405 22-23 22.325545285549772 26.191821471548536 29.81721019102786 21.665423051873834 24-25 22.113018933841538 26.42046942420887 29.91253056901342 21.55398107293617 26-27 21.85110835070908 26.64511951062015 29.947158018228514 21.556614120442255 28-29 21.707908438337594 26.806525154810686 29.925937336331987 21.55962907051973 30-31 21.89741646487612 26.52470470803527 29.828986364504583 21.748892462584028 32-33 21.79590503829399 26.588261027622202 29.814260275913913 21.801573658169897 34-35 22.213934946425383 26.589770605741325 29.695034412219574 21.50126003561372 36-37 22.224840878143155 26.41693931495961 29.71157815609654 21.646641650800692 38-39 21.961958631397994 26.79288588205574 29.639734560712768 21.605420925833503 40-41 22.135258375049794 26.748740958267312 29.46094498294937 21.655055683733522 42-43 22.038446287133517 26.698389428950225 29.277774131837408 21.98539015207885 44-45 22.07043260195814 26.871599598267377 29.1821475443169 21.875820255457587 46-47 22.33581027375737 27.165258754012704 28.59076261421961 21.908168358010315 48-49 22.394961089853787 27.178875764801568 28.465190360881593 21.960972784463053 50-51 22.42191784198106 27.41843656999205 28.203817692255555 21.955827895771332 52-53 22.50010782700851 27.660862492837207 27.859818727394835 21.97921095275945 54-55 22.382918943232074 27.481419067233325 28.015902967865962 22.119759021668642 56-57 22.374566381387208 27.689298015366887 27.99340098707924 21.942734616166657 58-59 22.575956425565476 27.31751049002754 28.04078325538981 22.06574982901717 60-61 22.433809620633777 27.485997892752177 28.296856996740544 21.783335489873505 62-63 22.269049951631885 27.51116779730987 28.38607614435264 21.83370610670561 64-65 22.21237059599703 27.536777493354002 28.21137427413146 22.039477636517507 66-67 22.281496269185507 27.485535777001424 28.092139719156854 22.14082823465622 68-69 22.266585334294533 27.388614700210105 28.117494470014847 22.227305495480508 70-71 22.36942149269549 27.448812978674898 28.034714135196587 22.147051393433024 72-73 22.47348995976512 27.448166016623844 27.845986062588956 22.232357961022075 74-75 22.239080894619402 27.841096212837023 27.641277300651673 22.2785455918919 76-77 22.239967467051148 27.59665921119922 27.87472966228581 22.288643659463823 78-79 22.390555586363273 27.532887237595272 27.79940479491303 22.277152381128428 80-81 22.268772682181435 27.821832812682924 27.66237207095633 22.247022434179314 82-83 22.305773213281157 27.89457047951245 27.632580203714074 22.16707610349232 84-85 22.331634184017073 27.656566453210136 27.570828524759257 22.44097083801354 86-87 22.132756612876392 27.669118960917334 27.708090722564187 22.490033703642087 88-89 22.367110913941723 27.68809651441493 27.47931261822461 22.465479953418733 90-91 22.371240651600363 27.67400509426736 27.507195105756733 22.447559148375543 92-93 22.36481609221253 27.521517191108885 27.596226985917284 22.517439730761303 94-95 22.209344596634566 27.64684498173102 27.50380475301453 22.640005668619875 96-97 22.117260331367802 27.64721467433163 27.55010875124001 22.685416243060562 98-99 22.233111166017952 27.65770500772975 27.22374242968518 22.885441396567117 100-101 22.1356075613274 27.567434489766928 27.53668335793732 22.76027459096835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 491.0 1 338.0 2 241.5 3 307.5 4 270.0 5 265.5 6 483.0 7 1036.0 8 1738.0 9 2165.0 10 2074.5 11 1862.0 12 1963.0 13 2198.5 14 2371.0 15 2581.0 16 2720.5 17 2811.5 18 3039.0 19 3252.0 20 3473.0 21 3890.5 22 4493.5 23 5361.0 24 6541.5 25 8019.0 26 10029.5 27 12242.0 28 14112.5 29 16215.0 30 19020.5 31 21907.5 32 24708.0 33 27494.5 34 30577.0 35 33731.5 36 38259.0 37 43013.0 38 45392.0 39 46710.0 40 48235.0 41 51010.0 42 53348.0 43 55506.0 44 58344.0 45 60607.5 46 61499.0 47 62763.5 48 65998.5 49 65124.5 50 61506.0 51 58400.0 52 55132.5 53 53336.5 54 52169.5 55 48755.0 56 43688.0 57 39537.5 58 35680.5 59 31396.5 60 26263.5 61 21326.5 62 16907.0 63 12686.0 64 9453.0 65 7192.0 66 5339.5 67 4142.0 68 3004.0 69 2007.0 70 1453.5 71 1013.5 72 808.0 73 646.5 74 490.0 75 384.0 76 246.0 77 134.0 78 90.5 79 62.0 80 41.0 81 27.5 82 19.0 83 15.5 84 8.5 85 5.5 86 4.0 87 2.5 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01583516639247799 2 0.0 3 0.0 4 0.0 5 0.03653795202622353 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033272334054233904 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.010006346389644E-4 24-25 1.8484630030129946E-4 26-27 0.0011090778018077968 28-29 0.0012323086686753298 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.853466176207816E-4 42-43 0.009889277066119521 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.5403858358441623E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.080771671688324E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.080771671688324E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.545389009038984E-4 84-85 6.161543343376648E-5 86-87 0.0 88-89 0.0 90-91 0.010197354233288354 92-93 0.0014479626856935125 94-95 0.0 96-97 0.0 98-99 0.0011706932352415632 100-101 0.016358897576665005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1622970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.191212024428786 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37780892828704 44.44517273307261 2 13.510094257881846 15.723375188608408 3 4.441448954474032 7.753598934164277 4 1.977556981630586 4.603057504338193 5 1.0386444475164842 3.021998963171371 6 0.6057047610468881 2.1148016504571263 7 0.4040678567477878 1.6459238826986924 8 0.2851689591371395 1.3275461891147964 9 0.2115018828900635 1.1076795819739433 >10 1.0544509879942399 10.95425468128339 >50 0.06381882349364949 2.5614391195987394 >100 0.027609541308228437 2.7736381733109825 >500 0.0014865323144237395 0.5417566647452408 >1k 4.247235184067827E-4 0.5495419452833225 >5k 2.1236175920339134E-4 0.8762147881788616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7570 0.46642883109361233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6611 0.40733963043063026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4260 0.2624817464278452 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1842 0.11349562838499788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1729 0.10653308440698227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.161543343376648E-5 0.0 16-17 0.0 0.0 0.0 6.161543343376648E-5 0.0 18-19 0.0 0.0 0.0 6.161543343376648E-5 0.0 20-21 0.0 0.0 0.0 9.242315015064973E-5 0.0 22-23 0.0 0.0 0.0 2.4646173373506593E-4 1.2323086686753297E-4 24-25 0.0 0.0 0.0 2.772694504519492E-4 1.2323086686753297E-4 26-27 0.0 0.0 0.0 5.237311841870152E-4 1.2323086686753297E-4 28-29 0.0 0.0 0.0 0.0012323086686753298 1.2323086686753297E-4 30-31 0.0 0.0 0.0 0.0034504642722909236 1.2323086686753297E-4 32-33 0.0 0.0 0.0 0.006808505394431197 1.2323086686753297E-4 34-35 0.0 0.0 0.0 0.009735238482535106 1.2323086686753297E-4 36-37 0.0 0.0 0.0 0.013894280239314345 1.2323086686753297E-4 38-39 0.0 0.0 0.0 0.01805332199609358 1.2323086686753297E-4 40-41 0.0 0.0 0.0 0.021380555401516975 1.2323086686753297E-4 42-43 0.0 0.0 0.0 0.02553959715829621 1.2323086686753297E-4 44-45 0.0 0.0 0.0 0.029452177181340383 1.2323086686753297E-4 46-47 0.0 0.0 0.0 0.03462787358977677 1.2323086686753297E-4 48-49 0.0 0.0 0.0 0.04091264780002095 1.2323086686753297E-4 50-51 0.0 0.0 0.0 0.04639642137562617 1.2323086686753297E-4 52-53 0.0 0.0 0.0 0.0564397370253301 1.2323086686753297E-4 54-55 0.0 0.0 0.0 0.07732736895937695 1.2323086686753297E-4 56-57 0.0 0.0 0.0 0.11204766569930436 1.2323086686753297E-4 58-59 0.0 0.0 0.0 0.15018761899480582 1.2323086686753297E-4 60-61 0.0 0.0 0.0 0.18650991700401118 1.2323086686753297E-4 62-63 0.0 0.0 0.0 0.22588217896818796 1.2323086686753297E-4 64-65 0.0 0.0 0.0 0.27797802793643756 1.8484630030129946E-4 66-67 0.0 0.0 0.0 0.3538266264934041 1.8484630030129946E-4 68-69 0.0 0.0 0.0 0.4488684325649889 1.8484630030129946E-4 70-71 0.0 0.0 0.0 0.5374714258427451 2.4646173373506593E-4 72-73 0.0 0.0 0.0 0.6347930029513793 2.4646173373506593E-4 74-75 0.0 0.0 0.0 0.7434210120951096 2.4646173373506593E-4 76-77 0.0 0.0 0.0 0.8677917644811672 2.4646173373506593E-4 78-79 0.0 0.0 0.0 0.9829818172855938 3.0807716716883246E-4 80-81 0.0 0.0 0.0 1.1112035342612618 3.0807716716883246E-4 82-83 0.0 0.0 0.0 1.2526417617084729 3.0807716716883246E-4 84-85 0.0 0.0 0.0 1.4337911360037463 3.0807716716883246E-4 86-87 0.0 0.0 0.0 1.648089613486386 3.0807716716883246E-4 88-89 0.0 0.0 0.0 1.9031467001854625 3.0807716716883246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6435 0.0 33.90432 1 GTATCAA 9200 0.0 30.689478 1 TATCAAC 11230 0.0 25.010265 2 ATCAACG 11200 0.0 24.992393 3 TCAACGC 11345 0.0 24.714855 4 GTACATG 6625 0.0 24.609293 1 CAACGCA 11455 0.0 24.436037 5 TACATGG 6665 0.0 24.385773 2 AACGCAG 11695 0.0 23.921297 6 ACATGGG 6680 0.0 23.477295 3 GAGTACT 7175 0.0 22.873674 12-13 CGTACAC 130 2.9323093E-4 21.93403 3 GTACTTT 7495 0.0 21.70494 14-15 ACGCAGA 13100 0.0 21.285145 7 AGTACTT 7325 0.0 21.173143 12-13 CGCAGAG 13230 0.0 21.004185 8 AGAGTAC 10850 0.0 20.248451 10-11 CATGGGA 3595 0.0 20.093485 4 GCAGAGT 13805 0.0 19.922874 9 GGGCGTA 295 3.0177034E-9 19.322754 9 >>END_MODULE