FastQCFastQC Report
Thu 26 May 2016
SRR937794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599598
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.41210354101467994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.3220809228318615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33760.21105302707305212No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21170.13234575187015737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.037.8926771
GTATCAA73450.035.7989581
TCAACGC89200.028.9872954
TATCAAC89650.028.8939042
ATCAACG89300.028.845673
CAACGCA90700.028.4546095
AACGCAG92350.027.9818926
GTACATG65100.026.805391
TACATGG65200.026.3159872
ACGCAGA104400.024.706687
CGCAGAG105100.024.5421288
ACATGGG65500.024.5249563
GCAGAGT110850.022.3263199
GAGTACT59200.022.1847512-13
AGTACTT61400.021.35117512-13
AGAGTAC92550.020.96574810-11
TAGGTCG1150.003710690420.6648465
GTACTTT63600.020.35090314-15
CATGGGA35650.019.7322164
CATGGGG33450.019.182774
Produced by FastQC (version 0.11.5)