##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937794_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1599598 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501063392177283 31.0 31.0 34.0 30.0 34.0 2 31.623552292513494 33.0 31.0 34.0 30.0 34.0 3 31.66279215152807 33.0 31.0 34.0 30.0 34.0 4 35.25504720561041 37.0 35.0 37.0 33.0 37.0 5 35.23277160886673 37.0 35.0 37.0 33.0 37.0 6 35.31391262054591 37.0 35.0 37.0 33.0 37.0 7 35.24383813933251 37.0 35.0 37.0 32.0 37.0 8 35.22711643800505 37.0 35.0 37.0 32.0 37.0 9 36.80869130869131 39.0 37.0 39.0 33.0 39.0 10-11 36.68727611562405 39.0 37.0 39.0 32.0 39.0 12-13 36.552371908442 39.0 37.0 39.0 32.0 39.0 14-15 37.64581444838015 40.0 37.0 41.0 32.0 41.0 16-17 37.57632011292837 40.0 37.0 41.0 32.0 41.0 18-19 37.52273446203358 40.0 37.0 41.0 32.0 41.0 20-21 37.3386450845775 40.0 37.0 41.0 32.0 41.0 22-23 37.186273676261166 39.5 37.0 41.0 31.0 41.0 24-25 37.02660262140863 39.0 36.0 41.0 31.0 41.0 26-27 37.12310624294354 39.0 37.0 41.0 31.0 41.0 28-29 37.06415111796839 39.0 36.5 41.0 31.0 41.0 30-31 36.85596818700699 39.0 36.0 41.0 30.5 41.0 32-33 36.612814594666915 39.0 36.0 41.0 30.0 41.0 34-35 36.33579624380626 39.0 35.0 40.0 30.0 41.0 36-37 36.33891515243205 39.0 35.0 41.0 30.0 41.0 38-39 36.28076460460691 39.0 35.0 41.0 30.0 41.0 40-41 36.08350660603477 39.0 35.0 41.0 29.5 41.0 42-43 35.827678266664506 39.0 35.0 40.5 28.5 41.0 44-45 35.58792927973153 39.0 35.0 40.0 27.0 41.0 46-47 35.225364435314376 38.5 35.0 40.0 25.5 41.0 48-49 34.8854421548414 38.0 34.0 40.0 24.0 41.0 50-51 33.988815940004926 37.5 33.5 39.5 23.5 40.5 52-53 34.09000667667752 38.0 33.5 39.5 23.0 40.5 54-55 34.37821440136834 38.0 34.0 40.0 23.0 41.0 56-57 34.08920585046993 38.0 33.5 40.0 22.5 41.0 58-59 33.8394027749472 38.0 33.0 40.0 21.5 41.0 60-61 33.51465524463022 37.5 33.0 40.0 20.0 41.0 62-63 33.12729510789586 37.0 32.5 40.0 18.5 41.0 64-65 32.74421948514565 36.5 32.0 39.0 17.5 41.0 66-67 32.30502038637208 36.0 31.5 39.0 13.5 40.5 68-69 31.809978507099906 35.0 31.0 39.0 9.5 40.0 70-71 31.354348717615302 35.0 31.0 38.0 7.5 40.0 72-73 30.868200010252576 35.0 30.0 37.5 6.5 39.5 74-75 30.35995137528304 34.5 29.5 37.0 2.0 39.0 76-77 29.87968414564159 34.0 29.0 36.0 2.0 39.0 78-79 29.327793295565513 34.0 29.0 36.0 2.0 38.5 80-81 28.78501942363019 34.0 28.5 35.0 2.0 37.0 82-83 28.250649538196473 34.0 27.0 35.0 2.0 37.0 84-85 27.749543635338377 33.5 26.0 35.0 2.0 36.0 86-87 27.209139421279595 33.0 25.5 35.0 2.0 36.0 88-89 26.692009492384962 33.0 25.0 35.0 2.0 35.5 90-91 26.147698359212754 32.0 24.0 35.0 2.0 35.0 92-93 25.5458280768043 32.0 21.5 34.0 2.0 35.0 94-95 24.876463961570344 31.5 18.5 34.0 2.0 35.0 96-97 24.057776703896856 31.0 6.0 34.0 2.0 35.0 98-99 23.07306992131773 31.0 2.0 34.0 2.0 35.0 100-101 20.56233316120675 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06383542053825408 1101 2 0.26120946338783213 1101 3 0.13178310535062465 1101 4 0.15691435297099332 1101 5 0.1496741071752936 1101 6 0.11362373044495655 1101 7 0.10419141503688678 1101 8 0.13394217489413052 1101 9 0.22926696302154426 1101 10-11 0.16809111912744612 1101 12-13 0.22803636166490548 1101 14-15 0.38238485513953435 1101 16-17 0.3405192350144901 1101 18-19 0.28690421219246076 1101 20-21 0.3789758863621273 1101 22-23 0.40882830843116835 1101 24-25 0.37685597468202303 1101 26-27 0.4837178018774395 1101 28-29 0.36524131498595835 1101 30-31 0.2770686433761185 1101 32-33 0.24682544826864472 1101 34-35 0.3943416638666335 1101 36-37 0.27113122560404435 1101 38-39 0.21402676973718116 1101 40-41 0.3928394853629129 1101 42-43 0.418815308538818 1101 44-45 0.34379668704252 1101 46-47 0.584785202960795 1101 48-49 0.7249989695457089 1101 50-51 0.5482916600106975 1101 52-53 0.7019340856144467 1101 54-55 0.7068204018196838 1101 56-57 0.6509990301410156 1101 58-59 0.752877181043921 1101 60-61 0.8177153063785383 1101 62-63 0.6739620300915803 1101 64-65 0.7167555261005134 1101 66-67 0.7204958354822963 1101 68-69 0.6755388911830167 1101 70-71 0.9238961872251679 1101 72-73 0.8141853678328204 1101 74-75 0.7238210707081265 1101 76-77 0.8883921888968374 1101 78-79 0.6912420858785886 1101 80-81 0.7043545409392742 1101 82-83 0.8977242465686537 1101 84-85 0.9060157092592114 1101 86-87 1.0290342233628955 1101 88-89 0.9664555464054736 1101 90-91 1.1554884314640397 1101 92-93 1.2710052715119318 1101 94-95 1.1942521238752484 1101 96-97 1.1534892961892496 1101 98-99 1.4031054573690263 1101 100-101 1.8853023412201217 1102 1 -0.028131821826328007 1102 2 0.2412022765218289 1102 3 0.09307634196072101 1102 4 -0.04434784037066919 1102 5 -0.06363513268885157 1102 6 0.11374881435411766 1102 7 0.017417806306845307 1102 8 -0.11916932633737076 1102 9 0.15762351642020178 1102 10-11 0.1004467704246963 1102 12-13 0.20267505320610724 1102 14-15 0.28266381409205366 1102 16-17 0.2505806290668673 1102 18-19 0.13449539555487178 1102 20-21 0.20334419702439277 1102 22-23 0.18348496713738172 1102 24-25 0.19827935470502922 1102 26-27 0.2553401620142921 1102 28-29 0.2997368057266314 1102 30-31 0.22474411021047302 1102 32-33 0.016251041746564 1102 34-35 0.13478335043442513 1102 36-37 0.2650515549521586 1102 38-39 0.35000030934354953 1102 40-41 0.2613349661218294 1102 42-43 0.3469419695657976 1102 44-45 0.4180040418516029 1102 46-47 0.24611100505746464 1102 48-49 0.32754001328827087 1102 50-51 0.29333805647116407 1102 52-53 0.29070919285596375 1102 54-55 0.36555562569804323 1102 56-57 0.4524947654347713 1102 58-59 0.2971161780269327 1102 60-61 0.37122065036241025 1102 62-63 0.4330510449169864 1102 64-65 0.2474578550942823 1102 66-67 0.3225867643098681 1102 68-69 0.40848349741512635 1102 70-71 0.3570570856832376 1102 72-73 0.3605535986410189 1102 74-75 0.17445831239893295 1102 76-77 0.36210897147667254 1102 78-79 0.30877214344837967 1102 80-81 0.3732472956799846 1102 82-83 0.3910384960277682 1102 84-85 0.31726586834348325 1102 86-87 0.5002184466947561 1102 88-89 0.3877629508738103 1102 90-91 0.5850337498946097 1102 92-93 0.8753771880843999 1102 94-95 0.7127859096317763 1102 96-97 0.7266757042784668 1102 98-99 0.9487618640358413 1102 100-101 1.126763414858047 1103 1 0.1244746804158261 1103 2 0.29119064271186446 1103 3 0.18088781078211014 1103 4 0.06519505665953318 1103 5 0.07987584539727521 1103 6 0.07917789041808021 1103 7 0.025535032806544677 1103 8 0.038167377275456715 1103 9 0.11161444204842752 1103 10-11 0.024566566938780454 1103 12-13 0.07638079184728497 1103 14-15 0.21066242861104456 1103 16-17 0.07414596877411128 1103 18-19 0.14420962687731986 1103 20-21 0.1586148417489852 1103 22-23 0.12384714879403447 1103 24-25 0.18182015764944737 1103 26-27 -0.0650166257850131 1103 28-29 -0.04723893249149569 1103 30-31 0.040537271645455064 1103 32-33 0.03023805418416714 1103 34-35 -0.01992516974494407 1103 36-37 0.22040284313221292 1103 38-39 0.2141517815493188 1103 40-41 0.38623978040391904 1103 42-43 0.2440409204076559 1103 44-45 0.19747856380777762 1103 46-47 0.23045956458026495 1103 48-49 0.1458423760093126 1103 50-51 0.08146654661415198 1103 52-53 0.07735833829974581 1103 54-55 0.34703935951647225 1103 56-57 0.5538794532256119 1103 58-59 0.48056946299181647 1103 60-61 0.42957610556167225 1103 62-63 0.4150358257783111 1103 64-65 0.501160843925831 1103 66-67 0.6149602730669557 1103 68-69 0.8015317671502942 1103 70-71 0.7908303004222219 1103 72-73 0.7182271539333449 1103 74-75 0.7236904809411122 1103 76-77 0.7207436381151382 1103 78-79 0.8037709271864273 1103 80-81 0.8178465638075778 1103 82-83 0.6410041234074093 1103 84-85 0.8841179708902303 1103 86-87 0.8651353707154001 1103 88-89 0.9475356156508603 1103 90-91 0.6522996459095012 1103 92-93 0.8179550817302221 1103 94-95 0.8200681458615762 1103 96-97 1.0782679498153165 1103 98-99 1.2290785151907606 1103 100-101 1.5703949615202006 1104 1 0.1388413576510814 1104 2 0.2880656801007433 1104 3 0.3273037874993072 1104 4 0.28610875115948176 1104 5 0.27993065749571855 1104 6 0.33581254721806886 1104 7 0.4034984754549953 1104 8 0.3262644029152568 1104 9 0.35874260215658893 1104 10-11 0.44434330297927005 1104 12-13 0.4490838059892468 1104 14-15 0.6243967477146697 1104 16-17 0.5218467769721542 1104 18-19 0.6429399005040963 1104 20-21 0.6334061370194419 1104 22-23 0.6562672338014011 1104 24-25 0.5726390189935344 1104 26-27 0.5145867305399818 1104 28-29 0.4280085071179869 1104 30-31 0.4127531086777978 1104 32-33 0.5846702816042821 1104 34-35 0.6025780008321036 1104 36-37 0.6668363552342456 1104 38-39 0.8011149520067207 1104 40-41 0.9852791852925904 1104 42-43 1.0513528393844567 1104 44-45 0.9317144771732586 1104 46-47 0.8202932663186999 1104 48-49 0.9369793045576671 1104 50-51 0.9831392129498013 1104 52-53 0.9461394458481109 1104 54-55 1.0416772706446054 1104 56-57 1.129782805961284 1104 58-59 1.1914373262023545 1104 60-61 0.9663557340059299 1104 62-63 1.0476880209542472 1104 64-65 1.0656433893009734 1104 66-67 0.9241760338259795 1104 68-69 1.1760315039325775 1104 70-71 1.11200844647216 1104 72-73 1.072007094948649 1104 74-75 1.159365903585016 1104 76-77 1.0980581967468837 1104 78-79 1.2359080531480728 1104 80-81 1.4403688027135502 1104 82-83 1.4976450615392842 1104 84-85 1.3154817078639454 1104 86-87 1.3428181350398312 1104 88-89 1.5595110122721074 1104 90-91 1.6177315697318981 1104 92-93 1.801803272407529 1104 94-95 1.8322034547236363 1104 96-97 1.7853219602961623 1104 98-99 2.106496827965195 1104 100-101 2.0188637373185294 1105 1 0.19180715464263187 1105 2 0.22647803816022005 1105 3 0.24612925213593329 1105 4 0.2833675911858933 1105 5 0.3332980881516079 1105 6 0.240193500221622 1105 7 0.2685446612834994 1105 8 0.31167933992863794 1105 9 0.3256231465806252 1105 10-11 0.36136570621129493 1105 12-13 0.3526189993944513 1105 14-15 0.3371790353023485 1105 16-17 0.49719717476516934 1105 18-19 0.5403813347162583 1105 20-21 0.6624017995393032 1105 22-23 0.523590024693668 1105 24-25 0.597674320263728 1105 26-27 0.4825878042869576 1105 28-29 0.4647054849370207 1105 30-31 0.6231725809238071 1105 32-33 0.36585490888170114 1105 34-35 0.5657707065437165 1105 36-37 0.5174237291466071 1105 38-39 0.5252537072447083 1105 40-41 0.646457879995431 1105 42-43 0.5369557229752573 1105 44-45 0.5332321866438008 1105 46-47 0.5932993574221257 1105 48-49 0.5613956487189071 1105 50-51 0.38950790529923296 1105 52-53 0.3067033100951164 1105 54-55 0.4435368794542356 1105 56-57 0.4000700046747454 1105 58-59 0.5346962136239952 1105 60-61 0.5384040330076871 1105 62-63 0.8392389234246806 1105 64-65 0.7846021734336972 1105 66-67 0.9018451203729256 1105 68-69 1.0505105650495352 1105 70-71 1.0916928514823212 1105 72-73 0.6417342537193811 1105 74-75 0.8360348691924244 1105 76-77 1.0398905696459693 1105 78-79 0.9059792767293189 1105 80-81 1.0624852527785933 1105 82-83 1.0646449520399734 1105 84-85 1.2071764711820023 1105 86-87 1.188367110649267 1105 88-89 0.9064109560515625 1105 90-91 0.9549614578137984 1105 92-93 1.1051833690284099 1105 94-95 1.3815165508411518 1105 96-97 1.3795408052909046 1105 98-99 1.606218176735208 1105 100-101 1.9070512540702893 1106 1 0.10322810038846697 1106 2 0.21769188164534725 1106 3 0.22229609744034562 1106 4 0.27012401156751764 1106 5 0.24463706032642563 1106 6 0.14207554289723845 1106 7 0.1631002058700659 1106 8 0.12959840997813643 1106 9 0.3700651621785198 1106 10-11 0.005223153289868776 1106 12-13 -0.156785925366151 1106 14-15 0.17890790198688222 1106 16-17 0.17131432821969383 1106 18-19 0.10690288759538191 1106 20-21 0.11145390299875402 1106 22-23 0.17178766385482191 1106 24-25 0.15987022475834323 1106 26-27 0.24307733635007622 1106 28-29 0.4216385098851916 1106 30-31 0.29526110885475276 1106 32-33 0.40172451625473826 1106 34-35 0.2965077987913034 1106 36-37 0.3284050144921977 1106 38-39 0.3594401239595655 1106 40-41 0.2392374186869617 1106 42-43 0.3781764416631077 1106 44-45 0.4599328297396852 1106 46-47 0.6710080991542569 1106 48-49 0.7876175837887871 1106 50-51 0.8148405593390322 1106 52-53 0.7231145071560547 1106 54-55 0.5663394588892672 1106 56-57 0.7169472170797349 1106 58-59 0.5615425581315847 1106 60-61 0.32789281761287725 1106 62-63 0.7674829613899092 1106 64-65 0.7894399362779865 1106 66-67 0.8862657346987319 1106 68-69 0.7757448508041165 1106 70-71 0.6714905971209788 1106 72-73 0.722688443714727 1106 74-75 0.8562708825620504 1106 76-77 0.8118951848461577 1106 78-79 0.6628386136071995 1106 80-81 0.5736887482021764 1106 82-83 0.5187296157544132 1106 84-85 0.5732021280282957 1106 86-87 0.5797834200404388 1106 88-89 1.0662744039481034 1106 90-91 1.1494358221219763 1106 92-93 1.063903946686473 1106 94-95 1.0701350934392018 1106 96-97 1.2277618906562395 1106 98-99 1.5590348944076524 1106 100-101 1.2370226426496949 1107 1 0.2578552511742309 1107 2 0.4727400179608061 1107 3 0.4604422423102754 1107 4 0.4518077183793352 1107 5 0.36121988997809495 1107 6 0.41551773065292963 1107 7 0.41942969549998566 1107 8 0.31633372923084835 1107 9 0.22339887342907616 1107 10-11 0.5506042382474376 1107 12-13 0.5765243942341698 1107 14-15 0.6629880496740768 1107 16-17 0.5850104228729833 1107 18-19 0.5895254691479579 1107 20-21 0.7273038049720242 1107 22-23 0.644830845889345 1107 24-25 0.6223724575004539 1107 26-27 0.5359510652914423 1107 28-29 0.5468734157463686 1107 30-31 0.448974038650924 1107 32-33 0.7108552816761673 1107 34-35 0.5671057137577904 1107 36-37 0.5678295713396864 1107 38-39 0.6648765702256085 1107 40-41 0.8611197352625126 1107 42-43 0.8376512365449713 1107 44-45 0.5706209528351707 1107 46-47 0.6591587897266749 1107 48-49 0.8314769279883052 1107 50-51 0.8051218474092892 1107 52-53 0.7152041826356808 1107 54-55 0.7085720833425739 1107 56-57 0.8517764965377523 1107 58-59 0.9078833745825392 1107 60-61 1.0949108581691007 1107 62-63 0.9292556891001809 1107 64-65 0.8043474339062016 1107 66-67 0.9899943872776475 1107 68-69 1.2838209597683488 1107 70-71 1.277579004082213 1107 72-73 1.054212943981586 1107 74-75 1.3022647282156612 1107 76-77 1.1772514770075517 1107 78-79 1.307675390800398 1107 80-81 1.3159393950289022 1107 82-83 1.4087375199288665 1107 84-85 1.3082654844252737 1107 86-87 1.4185810442958804 1107 88-89 1.6417263804730275 1107 90-91 1.505580649272975 1107 92-93 1.5109066708992387 1107 94-95 1.5249668543159984 1107 96-97 1.6121972983629682 1107 98-99 1.8950542922571607 1107 100-101 1.896914519104989 1108 1 0.2937681586766949 1108 2 0.23997721640614955 1108 3 0.2571986530025079 1108 4 0.3204828608706336 1108 5 0.3844167942624708 1108 6 0.2922777673664001 1108 7 0.25606894038106276 1108 8 0.24605293921147364 1108 9 0.2829143463415704 1108 10-11 0.33088236540747573 1108 12-13 0.23703928924952322 1108 14-15 0.3324810299235992 1108 16-17 0.3760071254599282 1108 18-19 0.37318146171461564 1108 20-21 0.38168874112005824 1108 22-23 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4 1621.0 5 1827.0 6 1997.0 7 2401.0 8 2942.0 9 3643.0 10 4699.0 11 5709.0 12 7228.0 13 8894.0 14 10316.0 15 12081.0 16 14675.0 17 17968.0 18 16043.0 19 12060.0 20 12298.0 21 13246.0 22 14501.0 23 16109.0 24 17730.0 25 20571.0 26 23590.0 27 27307.0 28 31696.0 29 37666.0 30 46123.0 31 56054.0 32 69733.0 33 88287.0 34 116978.0 35 159132.0 36 219378.0 37 278955.0 38 195751.0 39 21581.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77279271235158 18.707267583307836 14.784475623397928 22.73546408094266 2 15.22520150928259 19.63565366728091 41.26797287302721 23.871171950409288 3 19.336323701856458 24.20224283906599 29.658234345533597 26.80319911354396 4 12.603464550346793 16.27491265918467 37.439218689868966 33.68240410059957 5 13.672213617682214 37.21442311730331 34.25763016551848 14.85573309949599 6 31.900699508333403 35.98468310399541 17.47104591748846 14.643571470182728 7 28.58121769544335 32.63640258461508 20.588290561244303 18.194089158697267 8 26.187784399632964 35.122340775352086 19.497671563638185 19.19220326137677 9 26.065075617534323 17.118258770262134 20.589593138013353 36.22707247419019 10-11 24.99794847187778 26.686089887626373 27.115313420251308 21.200648220244542 12-13 25.86752665837535 23.89414760692399 28.009780453437454 22.228545281263205 14-15 22.70745271270825 25.315895896548007 26.538293372888706 25.43835801785504 16-17 21.870652967021268 28.781249314965535 27.060286164339374 22.28781155367382 18-19 21.787701624015995 27.17511537478641 29.618091919004648 21.41909108219295 20-21 22.69116265990437 26.109031991990967 29.801342960293333 21.398462387811335 22-23 22.314896181017264 25.995927537475488 29.737463335623648 21.9517129458836 24-25 22.09216604293566 26.203278893882214 29.80884079983715 21.895714263344974 26-27 21.82620013378664 26.40403940442398 29.870309638400272 21.89945082338911 28-29 21.708590653718538 26.52686853906332 29.907962874454796 21.85657793276335 30-31 21.88944984872876 26.269550946776327 29.861350354218995 21.97964885027592 32-33 21.7590569820406 26.276014285835092 29.860471001590135 22.104457730534172 34-35 22.23137785853947 26.36156161122078 29.650763107149448 21.756297423090306 36-37 22.166498556212513 26.17960459544357 29.63457975152638 22.01931709681753 38-39 21.882611762871367 26.50661827076717 29.63500486432647 21.975765102035 40-41 22.11836972276069 26.489296714603167 29.48600370913215 21.906329853503998 42-43 21.96055466479607 26.395367772321876 29.32132478204229 22.322752780839764 44-45 22.018953683040955 26.588330789431986 29.178475400359133 22.214240127167926 46-47 22.190363273438095 26.8338259361489 28.743922320951558 22.231888469461445 48-49 22.273103828439794 26.783293112817375 28.670781113218275 22.27282194552456 50-51 22.187476021728816 27.09734904063426 28.483257881706674 22.231917055930246 52-53 22.329414336992336 27.18933316292857 28.199106690611043 22.28214580946805 54-55 22.193964549408076 27.088721681130064 28.33059786116453 22.386715908297326 56-57 22.29648130909742 27.22438319817971 28.266880582934355 22.212254909788516 58-59 22.326865608083903 26.94185184350209 28.384534070784266 22.346748477629735 60-61 22.306483800048966 26.96074103434479 28.665895225518174 22.06687994008808 62-63 22.156498378908115 27.10568955503337 28.57470720935803 22.163104856700485 64-65 22.044316004358336 27.171747216551655 28.486981351835382 22.296955427254623 66-67 22.12365191608339 27.117382122399526 28.272683581281104 22.486282380235984 68-69 22.100264010861014 27.051344351319806 28.45086492362722 22.39752671419196 70-71 22.191881874914483 27.025359082237372 28.344647558823173 22.43811148402498 72-73 22.282786516966507 26.96484588667411 28.236696076304124 22.515671520055257 74-75 22.038450602430768 27.369201940504716 28.13359195038761 22.458755506676905 76-77 22.10881989351349 27.17964181355217 28.102662663715805 22.608875629218534 78-79 22.243630270583743 27.05420975023352 28.164870989631137 22.537288989551598 80-81 22.131384173555713 27.361307416622253 28.02918103602134 22.478127373800692 82-83 22.212378243373628 27.293050519647295 28.087595271801302 22.40697596517778 84-85 22.16275866387002 27.143662148201486 27.98528629120621 22.708292896722288 86-87 22.060724080497916 27.16879766571074 28.14628129187382 22.624196961917523 88-89 22.280133740227235 27.05869497766561 27.97167021556047 22.68950106654669 90-91 22.261496047829947 27.035974218920007 28.031116392151706 22.671413341098344 92-93 22.294998613004445 26.86215894155019 28.169034369311518 22.673808076133845 94-95 22.156719396980204 26.968479222273533 28.040858817912127 22.83394256283413 96-97 22.032465977102547 26.982173301125144 28.09391932193731 22.891441399834996 98-99 22.184764589949566 26.899666638902232 27.926898539361094 22.988670231787108 100-101 22.449952066944725 26.326956661129262 28.392398587618555 22.83069268430745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1988.0 1 1164.0 2 365.0 3 393.5 4 326.5 5 304.0 6 474.0 7 984.5 8 1697.0 9 2035.0 10 1862.5 11 1714.5 12 1787.0 13 1918.5 14 2074.0 15 2224.0 16 2371.5 17 2493.5 18 2646.5 19 2840.5 20 3153.0 21 3571.5 22 4198.0 23 5091.5 24 6185.0 25 7667.0 26 9487.5 27 11659.0 28 13755.5 29 15863.5 30 18545.5 31 21536.5 32 24403.0 33 27224.0 34 30563.0 35 33644.5 36 37893.5 37 42071.5 38 44090.0 39 45787.5 40 47595.5 41 50254.0 42 52631.0 43 55178.0 44 57779.0 45 59624.5 46 60833.0 47 62103.5 48 64553.0 49 64016.0 50 60934.0 51 57590.0 52 54234.0 53 52428.5 54 51279.0 55 48105.5 56 43312.0 57 38925.0 58 35055.5 59 30883.5 60 25905.5 61 21124.5 62 16716.0 63 12772.5 64 9802.5 65 7643.0 66 5685.5 67 4217.5 68 3127.0 69 2181.0 70 1571.0 71 1135.0 72 858.0 73 686.5 74 539.5 75 393.5 76 278.5 77 180.5 78 118.0 79 80.5 80 56.5 81 38.5 82 23.0 83 18.5 84 14.5 85 9.5 86 7.0 87 6.0 88 8.0 89 6.0 90 3.5 91 2.5 92 0.5 93 1.5 94 2.5 95 4.0 96 3.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2994502368720141 2 0.2586899958614602 3 0.2520008152048202 4 0.25781477596246055 5 0.26175326550795885 6 0.1994876212648428 7 0.1921732835374888 8 0.18961013954756134 9 0.20061290399212806 10-11 0.20173818671941324 12-13 0.19683070371430822 14-15 0.1991750427294858 16-17 0.1852340400525632 18-19 0.19142309505263197 20-21 0.181389324067672 22-23 0.1888599510627045 24-25 0.1889849824768473 26-27 0.18917252959806152 28-29 0.19861240136584318 30-31 0.1955491317193445 32-33 0.19951887911837848 34-35 0.19576793669409437 36-37 0.18313976386567127 38-39 0.17307473502717557 40-41 0.1705741067443195 42-43 0.1849214615172062 44-45 0.18560913429499162 46-47 0.18610925995156283 48-49 0.1994563634113071 50-51 0.19382994977488094 52-53 0.21245963048215863 54-55 0.18639058063338415 56-57 0.16951133972410568 58-59 0.17166813161806904 60-61 0.16172813419371618 62-63 0.16757335280489222 64-65 0.16616674939578568 66-67 0.1660104601281072 68-69 0.16841731485035616 70-71 0.1696988868453199 72-73 0.167073227148321 74-75 0.1812955505070649 76-77 0.1735436028302111 78-79 0.17795096017874493 80-81 0.17123052166856922 82-83 0.15360109227443394 84-85 0.15303845091079135 86-87 0.15866486454721748 88-89 0.16388492608767954 90-91 0.16819850987560625 92-93 0.16385366823414382 94-95 0.16476014598667915 96-97 0.16851108841096327 98-99 0.17698196671913818 100-101 0.16122800853714495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1599598.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.87383374998072 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21286068669998 47.61116678552577 2 12.685074084336042 15.443781818321224 3 4.067344275620387 7.4278451771415375 4 1.733813265059029 4.221754418028583 5 0.9421761746955413 2.867693791080459 6 0.5479965195681582 2.001518941665607 7 0.36634049565901267 1.5610385300042589 8 0.24179372696700713 1.1775128909742258 9 0.17396370918529935 0.9530854120328317 >10 0.9466210343881143 10.280037366678634 >50 0.058911028948893704 2.497893278269471 >100 0.021667470165276863 2.3404009814135205 >500 8.214449755484399E-4 0.36415780676160164 >1k 4.1072248777421997E-4 0.5180466705183925 >5k 2.0536124388710999E-4 0.7340661315839769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6592 0.41210354101467994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5152 0.3220809228318615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3376 0.21105302707305212 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2117 0.13234575187015737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.25157070714017E-5 2 0.0 0.0 0.0 0.0 6.25157070714017E-5 3 0.0 0.0 0.0 0.0 6.25157070714017E-5 4 0.0 0.0 0.0 0.0 6.25157070714017E-5 5 0.0 0.0 0.0 0.0 6.25157070714017E-5 6 0.0 0.0 0.0 0.0 6.25157070714017E-5 7 0.0 0.0 0.0 0.0 6.25157070714017E-5 8 0.0 0.0 0.0 0.0 6.25157070714017E-5 9 0.0 0.0 0.0 0.0 6.25157070714017E-5 10-11 0.0 0.0 0.0 0.0 6.25157070714017E-5 12-13 0.0 0.0 0.0 6.25157070714017E-5 6.25157070714017E-5 14-15 0.0 0.0 0.0 6.25157070714017E-5 6.25157070714017E-5 16-17 0.0 0.0 0.0 1.250314141428034E-4 6.25157070714017E-5 18-19 0.0 0.0 0.0 1.8754712121420508E-4 6.25157070714017E-5 20-21 0.0 0.0 0.0 2.1880497474990591E-4 6.25157070714017E-5 22-23 0.0 0.0 0.0 3.125785353570085E-4 6.25157070714017E-5 24-25 0.0 0.0 0.0 4.688678030355127E-4 6.25157070714017E-5 26-27 0.0 0.0 0.0 7.814463383925211E-4 6.25157070714017E-5 28-29 0.0 0.0 0.0 0.002031760479820555 6.25157070714017E-5 30-31 0.0 0.0 0.0 0.0045636466162123236 6.25157070714017E-5 32-33 0.0 0.0 0.0 0.007439369141496802 6.25157070714017E-5 34-35 0.0 0.0 0.0 0.011315342979923707 6.25157070714017E-5 36-37 0.0 0.0 0.0 0.016129052424421635 6.25157070714017E-5 38-39 0.0 0.0 0.0 0.02003628411638424 6.25157070714017E-5 40-41 0.0 0.0 0.0 0.023162069469954326 6.25157070714017E-5 42-43 0.0 0.0 0.0 0.026381628384131516 6.25157070714017E-5 44-45 0.0 0.0 0.0 0.029382382323558796 6.25157070714017E-5 46-47 0.0 0.0 0.0 0.03538389020241336 6.25157070714017E-5 48-49 0.0 0.0 0.0 0.04219810227319614 6.25157070714017E-5 50-51 0.0 6.25157070714017E-5 0.0 0.047730742349015184 9.377356060710254E-5 52-53 0.0 6.25157070714017E-5 0.0 0.06060897800572394 1.250314141428034E-4 54-55 0.0 6.25157070714017E-5 0.0 0.08408362601103528 1.250314141428034E-4 56-57 0.0 6.25157070714017E-5 0.0 0.12331223219833982 1.250314141428034E-4 58-59 0.0 6.25157070714017E-5 0.0 0.16551033447153596 1.250314141428034E-4 60-61 0.0 6.25157070714017E-5 0.0 0.2034886265174125 1.250314141428034E-4 62-63 0.0 6.25157070714017E-5 0.0 0.24681201151789386 1.8754712121420508E-4 64-65 0.0 6.25157070714017E-5 0.0 0.3046702984124761 1.8754712121420508E-4 66-67 0.0 6.25157070714017E-5 0.0 0.3860970068729768 2.1880497474990591E-4 68-69 0.0 6.25157070714017E-5 0.0 0.48634094316196946 2.500628282856068E-4 70-71 0.0 6.25157070714017E-5 0.0 0.5835841255115348 3.1257853535700844E-4 72-73 0.0 6.25157070714017E-5 0.0 0.685953595840955 3.1257853535700844E-4 74-75 0.0 6.25157070714017E-5 0.0 0.7935431277108373 3.1257853535700844E-4 76-77 0.0 6.25157070714017E-5 0.0 0.9209188808688182 3.438363888927093E-4 78-79 0.0 6.25157070714017E-5 0.0 1.0385421837236606 4.06352095964111E-4 80-81 0.0 6.25157070714017E-5 0.0 1.1664493203917483 4.3760994949981183E-4 82-83 0.0 6.25157070714017E-5 0.0 1.3074222398377593 5.001256565712135E-4 84-85 0.0 6.25157070714017E-5 0.0 1.4925625063297154 5.001256565712135E-4 86-87 0.0 6.25157070714017E-5 0.0 1.7075227650947302 5.001256565712135E-4 88-89 0.0 6.25157070714017E-5 0.0 1.965306283203655 5.001256565712135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4555 0.0 37.892677 1 GTATCAA 7345 0.0 35.798958 1 TCAACGC 8920 0.0 28.987295 4 TATCAAC 8965 0.0 28.893904 2 ATCAACG 8930 0.0 28.84567 3 CAACGCA 9070 0.0 28.454609 5 AACGCAG 9235 0.0 27.981892 6 GTACATG 6510 0.0 26.80539 1 TACATGG 6520 0.0 26.315987 2 ACGCAGA 10440 0.0 24.70668 7 CGCAGAG 10510 0.0 24.542128 8 ACATGGG 6550 0.0 24.524956 3 GCAGAGT 11085 0.0 22.326319 9 GAGTACT 5920 0.0 22.18475 12-13 AGTACTT 6140 0.0 21.351175 12-13 AGAGTAC 9255 0.0 20.965748 10-11 TAGGTCG 115 0.0037106904 20.664846 5 GTACTTT 6360 0.0 20.350903 14-15 CATGGGA 3565 0.0 19.732216 4 CATGGGG 3345 0.0 19.18277 4 >>END_MODULE