FastQCFastQC Report
Thu 26 May 2016
SRR937794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599598
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75390.47130591561129737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.3970997713175435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.27175577863938316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18260.11415368111237949No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.11346600833459407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.034.819941
GTATCAA83900.033.4169041
ATCAACG103350.026.7091663
TCAACGC104050.026.5294784
TATCAAC104300.026.4203362
CAACGCA105900.026.1557545
AACGCAG108600.025.5420326
GTACATG66550.023.1351911
TACATGG65800.023.1056792
ACGCAGA122250.022.692237
GAGTACT70900.022.6455912-13
CGCAGAG123050.022.5060948
ACATGGG65300.022.482263
AGTACTT73650.021.4130512-13
GTACTTT73900.021.30647314-15
GCAGAGT130400.021.1282529
AGAGTAC105750.020.70778810-11
GTATACG1456.130105E-419.6634351
ACTGCGT1456.145615E-419.656068
TACTTTT83200.018.69650514-15
Produced by FastQC (version 0.11.5)