FastQCFastQC Report
Thu 26 May 2016
SRR937793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504837
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.42901656458473575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.3448878516410747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.21703347272827556No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19620.13037956934870687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.035.603961
GTATCAA74250.032.596581
ATCAACG90450.026.8492073
TCAACGC91250.026.5590824
GTACATG63150.026.4292281
TATCAAC91750.026.3678232
CAACGCA92050.026.1733865
AACGCAG93900.025.6989126
TACATGG64350.025.4081152
ACATGGG65600.024.1970143
GAGTACT58100.022.85037412-13
CTTAGCG1252.2484297E-422.8118443
ACGCAGA105750.022.6844167
CGCAGAG106300.022.4776738
CATGGGG32650.021.831514
GTATAGG3500.021.7371521
AACCGCT1754.048754E-621.7154357
GTACTTT61900.021.48633214-15
GCAGAGT112400.020.7922239
AGTACTT60400.020.44674112-13
Produced by FastQC (version 0.11.5)