##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937793_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1504837 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.611752635002993 33.0 31.0 34.0 30.0 34.0 2 31.74178665197626 33.0 31.0 34.0 30.0 34.0 3 31.757583047200463 33.0 31.0 34.0 30.0 34.0 4 35.35977185568935 37.0 35.0 37.0 33.0 37.0 5 35.351628116533554 37.0 35.0 37.0 33.0 37.0 6 35.39388385585947 37.0 35.0 37.0 33.0 37.0 7 35.33078532758033 37.0 35.0 37.0 33.0 37.0 8 35.312239132876186 37.0 35.0 37.0 33.0 37.0 9 36.91723356084413 39.0 37.0 39.0 33.0 39.0 10-11 36.79709197740353 39.0 37.0 39.0 33.0 39.0 12-13 36.66344594132122 39.0 37.0 39.0 32.0 39.0 14-15 37.786936060184594 40.0 38.0 41.0 32.5 41.0 16-17 37.71042445128609 40.0 38.0 41.0 32.0 41.0 18-19 37.64440666995827 40.0 37.0 41.0 32.0 41.0 20-21 37.554118485922395 40.0 37.0 41.0 32.0 41.0 22-23 37.34690368458511 40.0 37.0 41.0 32.0 41.0 24-25 37.1743766932897 39.5 37.0 41.0 31.0 41.0 26-27 37.112064296664684 39.0 36.5 41.0 31.0 41.0 28-29 37.1519221018622 39.5 37.0 41.0 31.0 41.0 30-31 36.9745191671922 39.0 36.5 41.0 31.0 41.0 32-33 36.73639038646711 39.0 36.0 41.0 30.0 41.0 34-35 36.74528404072999 39.0 36.0 41.0 30.0 41.0 36-37 36.64792100406888 39.0 36.0 41.0 30.0 41.0 38-39 36.50954355853823 39.0 35.5 41.0 30.0 41.0 40-41 36.26117612738123 39.0 35.0 41.0 30.0 41.0 42-43 35.9987015204969 39.0 35.0 41.0 29.0 41.0 44-45 35.754631897009446 39.0 35.0 40.5 27.5 41.0 46-47 35.40955664965708 39.0 35.0 40.0 26.5 41.0 48-49 35.049095350526336 38.5 35.0 40.0 24.5 41.0 50-51 34.10781765732767 37.5 33.5 39.5 23.5 40.5 52-53 34.20682273229592 38.0 33.5 39.5 24.0 40.5 54-55 34.547738392928935 38.0 34.0 40.0 24.0 41.0 56-57 34.33128471721522 38.0 34.0 40.0 23.0 41.0 58-59 34.06837451498069 38.0 34.0 40.0 22.5 41.0 60-61 33.73249062855312 38.0 33.0 40.0 21.5 41.0 62-63 33.351927152243064 37.0 33.0 40.0 19.5 41.0 64-65 32.96981965488621 36.5 32.0 39.5 18.5 41.0 66-67 32.54764403054949 36.0 32.0 39.0 17.0 41.0 68-69 32.049609691946706 35.5 31.0 39.0 12.5 40.0 70-71 31.602743685860993 35.0 31.0 38.5 8.0 40.0 72-73 31.130190180066013 35.0 30.5 37.5 7.0 40.0 74-75 30.657113029517483 35.0 30.0 37.0 4.5 39.0 76-77 30.145943713505183 34.0 29.5 36.5 2.0 39.0 78-79 29.605640345100497 34.0 29.0 36.0 2.0 38.5 80-81 29.065918767281772 34.0 29.0 35.5 2.0 37.0 82-83 28.52336199867494 34.0 27.5 35.0 2.0 37.0 84-85 28.041459307552913 34.0 26.5 35.0 2.0 36.0 86-87 27.55570802684942 33.0 26.0 35.0 2.0 36.0 88-89 27.049461835401445 33.0 25.5 35.0 2.0 35.5 90-91 26.531369842713865 32.5 25.0 35.0 2.0 35.0 92-93 25.936286787206853 32.0 23.5 35.0 2.0 35.0 94-95 25.266908974194546 32.0 19.5 34.0 2.0 35.0 96-97 24.49692657742998 31.5 12.5 34.0 2.0 35.0 98-99 23.525048227814708 31.0 2.0 34.0 2.0 35.0 100-101 21.152217150428918 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01728482661708952 1101 2 0.1752745276439427 1101 3 0.0890665274423057 1101 4 0.15929311659142087 1101 5 0.011074116408181567 1101 6 0.051200798652438095 1101 7 -0.021131616671745235 1101 8 -0.01722974640337327 1101 9 0.08516826117613618 1101 10-11 0.10151947266227523 1101 12-13 0.061731375573380376 1101 14-15 0.29174568143591983 1101 16-17 0.31401733873727977 1101 18-19 0.32009564067164575 1101 20-21 0.3797632666371129 1101 22-23 0.4156231552620966 1101 24-25 0.37205562894914124 1101 26-27 0.435702105589435 1101 28-29 0.16335302758080417 1101 30-31 0.22300918819901483 1101 32-33 0.16648190811394414 1101 34-35 0.24071503856436038 1101 36-37 0.3211246577899587 1101 38-39 0.3563908352950733 1101 40-41 0.3854417991394783 1101 42-43 0.24552795338656352 1101 44-45 0.3322957459659719 1101 46-47 0.656743633592825 1101 48-49 0.5159541266213452 1101 50-51 0.5281939851285813 1101 52-53 0.45248299223649013 1101 54-55 0.4718713195969215 1101 56-57 0.5257630326038338 1101 58-59 0.47803607870370257 1101 60-61 0.4757524772454218 1101 62-63 0.6401606839462843 1101 64-65 0.5949622486705124 1101 66-67 0.7029255484497412 1101 68-69 0.5850839105910701 1101 70-71 0.6054592048097795 1101 72-73 0.6349225533656231 1101 74-75 0.670800223306788 1101 76-77 0.8764181866432956 1101 78-79 0.8508004267033265 1101 80-81 0.7935810737021995 1101 82-83 0.8672160398253261 1101 84-85 0.9832951055044532 1101 86-87 1.1512315628767489 1101 88-89 1.1330171114063958 1101 90-91 1.2896391261994502 1101 92-93 1.425141411962489 1101 94-95 1.7439474400553152 1101 96-97 1.7686643482630053 1101 98-99 1.826635930704203 1101 100-101 2.231306796435181 1102 1 0.2400634935208572 1102 2 0.22232707498990223 1102 3 0.33038643497320663 1102 4 0.249689814970246 1102 5 0.27673829352207946 1102 6 0.12826060719397958 1102 7 0.21391180231405826 1102 8 0.2820448749771387 1102 9 0.4356120832323853 1102 10-11 0.2719530697696513 1102 12-13 0.3769902594238914 1102 14-15 0.4403160409391873 1102 16-17 0.42349356943583416 1102 18-19 0.5221024721229952 1102 20-21 0.49075656855465866 1102 22-23 0.47180183994838387 1102 24-25 0.5181397699052468 1102 26-27 0.5485848002339324 1102 28-29 0.39684343983888226 1102 30-31 0.6210086547234113 1102 32-33 0.6351139136857995 1102 34-35 0.5537607396402038 1102 36-37 0.7345708360300804 1102 38-39 0.7155110451288138 1102 40-41 0.7093885553114916 1102 42-43 0.8974188279901654 1102 44-45 0.8862808827431863 1102 46-47 0.9882687069457248 1102 48-49 1.0066842472461062 1102 50-51 1.0662782149972827 1102 52-53 0.8146099297902154 1102 54-55 0.9014021574499722 1102 56-57 0.9528435874184495 1102 58-59 0.846502855029236 1102 60-61 0.9907553669048852 1102 62-63 0.9780307826392729 1102 64-65 1.0166172826054876 1102 66-67 0.9369564752154034 1102 68-69 0.9989035217466373 1102 70-71 1.1115308239082005 1102 72-73 1.0962088963904328 1102 74-75 1.1928301720338546 1102 76-77 1.118423091655007 1102 78-79 1.1165901928631925 1102 80-81 1.134157820093833 1102 82-83 1.1158338193813577 1102 84-85 1.2112714547531418 1102 86-87 1.203209868202613 1102 88-89 1.2719260056300392 1102 90-91 1.2055333646630437 1102 92-93 1.187393938562174 1102 94-95 1.5291653500164912 1102 96-97 1.8409799287140913 1102 98-99 2.0640384973145594 1102 100-101 2.4509405506214783 1103 1 0.30618150594321847 1103 2 0.45008414883393044 1103 3 0.5165044473955618 1103 4 0.46097000820696366 1103 5 0.32419516032677365 1103 6 0.3572909729634759 1103 7 0.41215886925677125 1103 8 0.49937751817935094 1103 9 0.38440090310816544 1103 10-11 0.4184403023438392 1103 12-13 0.5045817017979388 1103 14-15 0.6480023763429159 1103 16-17 0.624137116709818 1103 18-19 0.5847163437586005 1103 20-21 0.6039691289411309 1103 22-23 0.6912945935715697 1103 24-25 0.6443060915063512 1103 26-27 0.6443991549613273 1103 28-29 0.4589621423923731 1103 30-31 0.5683378472700014 1103 32-33 0.5420618087858671 1103 34-35 0.6150202289431732 1103 36-37 0.6928541348568586 1103 38-39 0.9751901341557243 1103 40-41 0.8387001495143949 1103 42-43 0.883312547796919 1103 44-45 0.6861549338128867 1103 46-47 0.7672490382086607 1103 48-49 0.8630921147409296 1103 50-51 0.943524591808881 1103 52-53 0.8702724556701256 1103 54-55 0.8717817295686814 1103 56-57 1.0311355128842905 1103 58-59 0.9908455051327607 1103 60-61 1.161333351722 1103 62-63 1.2857844058276768 1103 64-65 1.1698867788097616 1103 66-67 1.0302018168303064 1103 68-69 1.2908790220917048 1103 70-71 1.1297692642118626 1103 72-73 1.1693679716147223 1103 74-75 1.2148298269682947 1103 76-77 1.0486939681215333 1103 78-79 1.043434568294522 1103 80-81 1.1725498145727826 1103 82-83 1.4167982776284234 1103 84-85 1.273659308445346 1103 86-87 1.535231952398611 1103 88-89 1.5325574756093339 1103 90-91 1.6577918187693221 1103 92-93 1.6291106397353978 1103 94-95 1.5801953707204248 1103 96-97 1.8432918679825505 1103 98-99 1.9885864614277402 1103 100-101 2.594499901898221 1104 1 0.2048978443778644 1104 2 0.2830630322353507 1104 3 0.32459907577187863 1104 4 0.2135777760259998 1104 5 0.22854895989183888 1104 6 0.1387721834587694 1104 7 0.13784185606652244 1104 8 0.23780037857404324 1104 9 0.3304460629561419 1104 10-11 0.45333207723561486 1104 12-13 0.4005309325359079 1104 14-15 0.5256368701745942 1104 16-17 0.529793479126738 1104 18-19 0.5832300522723131 1104 20-21 0.688964149569955 1104 22-23 0.6793341440054945 1104 24-25 0.6091470564881618 1104 26-27 0.6716054228718207 1104 28-29 0.5766080176438848 1104 30-31 0.7161904172352465 1104 32-33 0.5694979407290433 1104 34-35 0.6048820259880685 1104 36-37 0.6698061499778873 1104 38-39 0.761005023491748 1104 40-41 0.6627490896196733 1104 42-43 0.7061522120450405 1104 44-45 0.7317058148567313 1104 46-47 0.8544345756758887 1104 48-49 0.651945030213561 1104 50-51 0.6753527323694186 1104 52-53 0.6584223652284891 1104 54-55 0.8743728317372472 1104 56-57 0.8654486645213808 1104 58-59 1.0182629390290714 1104 60-61 1.0659365263251743 1104 62-63 1.146147188584827 1104 64-65 0.9052220225957157 1104 66-67 1.0380295915594857 1104 68-69 1.2358423373367096 1104 70-71 1.0865802310228254 1104 72-73 1.144561760611328 1104 74-75 1.3675709199910777 1104 76-77 1.4898652381660433 1104 78-79 1.3726153093062479 1104 80-81 1.4380293585030302 1104 82-83 1.564710600047789 1104 84-85 1.4127504207758932 1104 86-87 1.6513996657494232 1104 88-89 1.7660257600635347 1104 90-91 1.7885060028637874 1104 92-93 1.7323478407190755 1104 94-95 1.723096006860498 1104 96-97 2.0944716740919347 1104 98-99 2.2128412832881956 1104 100-101 2.5038243333353307 1105 1 0.2709569475274627 1105 2 0.21876867537265454 1105 3 0.2807357960560708 1105 4 0.21916349743209196 1105 5 0.11790359464043121 1105 6 0.12967017865767616 1105 7 0.24474462065958136 1105 8 0.19123009781734623 1105 9 0.24454413142195364 1105 10-11 0.2504747557400506 1105 12-13 0.2080626301619546 1105 14-15 0.3676973611685739 1105 16-17 0.3975136805402997 1105 18-19 0.22737785769935215 1105 20-21 0.27446584298667176 1105 22-23 0.3944980170776091 1105 24-25 0.3540654854060463 1105 26-27 0.31316643078500306 1105 28-29 0.30026495237468964 1105 30-31 0.33081967081096764 1105 32-33 0.2650532281871918 1105 34-35 0.2675363312116872 1105 36-37 0.4581374256147015 1105 38-39 0.5297391614695428 1105 40-41 0.6324996298750847 1105 42-43 0.5798707015490265 1105 44-45 0.5674747739910444 1105 46-47 0.6566385711882887 1105 48-49 0.7414530675585524 1105 50-51 0.551898426441312 1105 52-53 0.6508728792416676 1105 54-55 0.7686034146847263 1105 56-57 0.7227212861206809 1105 58-59 0.8120522473436296 1105 60-61 0.8424152058299939 1105 62-63 0.7643997199037855 1105 64-65 0.7673278827157901 1105 66-67 0.8019437001953023 1105 68-69 0.9950614635231183 1105 70-71 1.0252134684868786 1105 72-73 0.942491265865943 1105 74-75 0.9448780791898592 1105 76-77 1.144623096306507 1105 78-79 1.2776065296054107 1105 80-81 1.4918633666786292 1105 82-83 1.4923841818663206 1105 84-85 1.5833883536626985 1105 86-87 1.712828511425709 1105 88-89 1.4776412074055827 1105 90-91 1.6912137964554006 1105 92-93 1.7485973321656942 1105 94-95 1.8875441185643602 1105 96-97 2.1490788595329526 1105 98-99 2.4427505626482358 1105 100-101 2.926350298177777 1106 1 0.3479245046288888 1106 2 0.3369075236190895 1106 3 0.23991057417748607 1106 4 0.3164264837908277 1106 5 0.32582441924902383 1106 6 0.05902386144795457 1106 7 0.11003168292576504 1106 8 0.01817137117702572 1106 9 0.1107905502621449 1106 10-11 0.048511579262246585 1106 12-13 0.12274012865064066 1106 14-15 -0.016686482215369836 1106 16-17 0.10659920345534601 1106 18-19 0.2512960626950118 1106 20-21 0.2188116625575489 1106 22-23 0.3404170281080212 1106 24-25 0.2761977101495603 1106 26-27 0.18581786894374375 1106 28-29 0.489207820281365 1106 30-31 0.5284788845766428 1106 32-33 0.48301115667789674 1106 34-35 0.5283811125747491 1106 36-37 0.6205057878747553 1106 38-39 0.361200143254905 1106 40-41 0.41736610846697886 1106 42-43 0.3442325761054832 1106 44-45 0.5817638206797326 1106 46-47 0.6807188592580999 1106 48-49 0.8292884042970883 1106 50-51 0.8430751933658485 1106 52-53 0.6636495156236251 1106 54-55 0.6252475065376402 1106 56-57 0.6246796439124935 1106 58-59 0.5764349298623088 1106 60-61 0.6252518129493794 1106 62-63 1.088751497122793 1106 64-65 0.9934031067846902 1106 66-67 0.9191905439559704 1106 68-69 1.1294036909826168 1106 70-71 1.2404597909533415 1106 72-73 1.133975443053405 1106 74-75 1.454205522044628 1106 76-77 1.353626482528572 1106 78-79 1.427561299666479 1106 80-81 1.283195098163926 1106 82-83 1.0124594847864472 1106 84-85 1.0606841289892728 1106 86-87 1.3068399798983599 1106 88-89 1.4444640067993042 1106 90-91 1.6065303184154658 1106 92-93 1.4977611806311621 1106 94-95 1.498020160933752 1106 96-97 1.6183942337696315 1106 98-99 1.7007523192783118 1106 100-101 2.3274040571909147 1107 1 0.1848550829951705 1107 2 0.13633920698088176 1107 3 0.3115387094568547 1107 4 0.19913411826296823 1107 5 0.17831275282274106 1107 6 0.13471256443911983 1107 7 0.22209683811366432 1107 8 0.2539878814383627 1107 9 0.27774490347918857 1107 10-11 0.31627118341796745 1107 12-13 0.1676640119072772 1107 14-15 0.48694749907182455 1107 16-17 0.435333332230357 1107 18-19 0.49994098925351693 1107 20-21 0.397193704868414 1107 22-23 0.4082672418955724 1107 24-25 0.43552410488652527 1107 26-27 0.35986891043108926 1107 28-29 0.22266661906981966 1107 30-31 0.424848334591303 1107 32-33 0.19265904695067348 1107 34-35 0.1051483493415617 1107 36-37 0.34359885641239174 1107 38-39 0.5114731353035893 1107 40-41 0.2072384175121229 1107 42-43 0.35829509791460623 1107 44-45 0.3374443699671872 1107 46-47 0.3382365508177898 1107 48-49 0.2566573401980463 1107 50-51 0.20052321205074009 1107 52-53 0.26351697432005494 1107 54-55 0.22494485408315512 1107 56-57 0.21010452050194317 1107 58-59 0.1326762934500394 1107 60-61 0.22828130353481413 1107 62-63 0.4537707705707916 1107 64-65 0.5390103425279236 1107 66-67 0.5295420837381428 1107 68-69 0.5819525666787619 1107 70-71 0.8107817052243043 1107 72-73 0.7198571480498437 1107 74-75 0.6755146044098268 1107 76-77 0.9990261535888543 1107 78-79 1.1710485142868485 1107 80-81 0.9848431117309424 1107 82-83 0.9944823709881945 1107 84-85 1.1375479074690773 1107 86-87 1.1244895497643164 1107 88-89 1.2636108455816242 1107 90-91 1.3543229444896383 1107 92-93 1.4009452542727132 1107 94-95 1.6670315505295754 1107 96-97 1.8315836346526986 1107 98-99 1.939051289189866 1107 100-101 2.5247207791461257 1108 1 0.1233543931161023 1108 2 -0.060803882054099034 1108 3 0.10857222946334488 1108 4 0.08907811631507201 1108 5 0.004199450331064725 1108 6 0.09214296781546949 1108 7 -0.13296768586977947 1108 8 0.041200770002603804 1108 9 0.06501866372592247 1108 10-11 0.06014343404697087 1108 12-13 0.18429212650836746 1108 14-15 0.18688054022108247 1108 16-17 0.2185794161521173 1108 18-19 0.17110201514427814 1108 20-21 0.3051252850972901 1108 22-23 0.23366696594394654 1108 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-1.013277564418587 2316 100-101 -1.054219019298575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6697.0 3 1436.0 4 1399.0 5 1575.0 6 1806.0 7 2014.0 8 2504.0 9 3146.0 10 3908.0 11 4961.0 12 6467.0 13 8008.0 14 9043.0 15 10928.0 16 13320.0 17 16711.0 18 14644.0 19 11274.0 20 11002.0 21 11858.0 22 12774.0 23 14291.0 24 15824.0 25 18029.0 26 20741.0 27 24180.0 28 28365.0 29 33656.0 30 41143.0 31 50071.0 32 62738.0 33 81342.0 34 107754.0 35 149133.0 36 209291.0 37 271548.0 38 198516.0 39 22736.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.850209648914706 18.73145746646017 14.729602213508693 22.68873067111643 2 15.159787292307836 19.557260972029646 41.361998189012944 23.920953546649574 3 19.32471747752045 24.22201806624913 29.713043901158603 26.740220555071815 4 12.620834732191867 16.307026799464968 37.51258972678295 33.55954874156022 5 13.642406956188111 37.13439580305366 34.3753538642307 14.847843376527528 6 31.849386214666392 36.017173737605106 17.508063506606618 14.625376541121888 7 28.50305971555398 32.6573617934707 20.62782926314486 18.211749227830463 8 26.14258575261138 35.15122943205812 19.558846736996028 19.14733807833447 9 26.16308453875151 17.056309226301654 20.619126478847168 36.16147975609967 10-11 24.89017907619245 26.690130200304573 27.215446112335968 21.20424461116701 12-13 25.821665487535896 23.917699446502144 28.031994673103334 22.22864039285863 14-15 22.70057665987388 25.29885869885477 26.58611428592355 25.414450355347796 16-17 21.83629958164679 28.694582182800975 27.121279815905947 22.347838419646287 18-19 21.68757087087867 27.150696041085297 29.709005753566764 21.452727334469266 20-21 22.51857320997131 26.098697162305122 29.942298051502338 21.44043157622123 22-23 22.248639631039445 25.97046531475217 29.84343940682987 21.937455647378517 24-25 22.01810951520116 26.169058312532435 29.918745841345523 21.894086330920885 26-27 21.828513789504697 26.38007400342553 29.969290076450232 21.822122130619544 28-29 21.655010762497565 26.461993317762534 30.016930332596964 21.866065587142934 30-31 21.848686339502237 26.23462379996724 29.93952727132293 21.977162589207598 32-33 21.6869275004861 26.270552715901736 29.93568161901997 22.106838164592197 34-35 22.170911841434165 26.34622888121545 29.755363675224487 21.72749560212589 36-37 22.06960234317969 26.16963419939863 29.76635776897284 21.994405688448843 38-39 21.731889620406204 26.498166176559685 29.782504345718824 21.987439857315287 40-41 22.027677226618735 26.459461933411554 29.625650115252434 21.887210724717278 42-43 21.87042053968472 26.3765262648382 29.427890790045748 22.325162405431335 44-45 21.898787733730842 26.565065812972716 29.31396121826485 22.22218523503159 46-47 22.080467162537516 26.865307948580696 28.807146984961424 22.247077903920363 48-49 22.257064249749313 26.772637626193692 28.708007015310365 22.26229110874663 50-51 22.165684366274746 27.10449896021101 28.530305038065666 22.199511635448584 52-53 22.283683470427952 27.13607713482845 28.301583966472617 22.278655428270984 54-55 22.14412979618143 26.989348329209733 28.47757276666463 22.38894910794421 56-57 22.1976269377939 27.239826654831646 28.379962864115882 22.18258354325858 58-59 22.358729881909078 26.89715286454191 28.466329010455183 22.277788243093834 60-61 22.264293122339783 26.974203615168424 28.724772437702452 22.03673082478935 62-63 22.122251569877655 27.005623765276454 28.712991580163205 22.159133084682686 64-65 22.007926718459707 27.077318788146666 28.61852900394805 22.296225489445575 66-67 22.02867147298278 27.130689193777087 28.422935846996715 22.41770348624342 68-69 21.991253200829878 26.97551075974795 28.607139302712547 22.426096736709628 70-71 22.153695816202195 26.958291826412147 28.432005256082586 22.456007101303072 72-73 22.216345648176873 26.920851978234563 28.404810817215843 22.45799155637273 74-75 21.951424906252225 27.311174708038845 28.280972380604315 22.45642800510462 76-77 22.073643172142006 27.15173761253426 28.199188844497904 22.575430370825835 78-79 22.140098676982696 27.038858223414806 28.303548243190157 22.51749485641234 80-81 22.02679935965955 27.313968882436807 28.156937337780253 22.502294420123388 82-83 22.164201188848644 27.248248467169518 28.190599261072347 22.396951082909496 84-85 22.190279235405388 27.070666119453012 28.04303549133229 22.69601915380931 86-87 22.022188787771867 27.076545659106188 28.21950456358056 22.681760989541377 88-89 22.167073953827916 27.052455989850944 28.07055738255 22.70991267377114 90-91 22.122415854121734 27.02035492881417 28.13155887789739 22.7256703391667 92-93 22.192348623016443 26.81793352438086 28.262540448361413 22.72717740424128 94-95 22.048140233144927 26.9317009193979 28.197487467295133 22.822671380162042 96-97 21.95944135591776 26.975036353935728 28.230757376711978 22.83476491343453 98-99 22.058694300462946 26.90287473321075 28.030013540844216 23.008417425482083 100-101 22.317837834959278 26.37790492749726 28.43757727973341 22.86667995781005 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1955.0 1 1149.5 2 346.0 3 361.5 4 322.5 5 293.0 6 467.0 7 932.5 8 1602.5 9 1993.0 10 1858.0 11 1671.0 12 1712.0 13 1814.5 14 1972.0 15 2127.5 16 2246.5 17 2373.0 18 2505.0 19 2711.5 20 2953.0 21 3333.0 22 4061.5 23 4995.0 24 5992.5 25 7379.0 26 9128.5 27 11277.0 28 13159.0 29 15066.0 30 17654.5 31 20415.0 32 23150.5 33 25749.5 34 28715.5 35 31853.5 36 35692.5 37 39630.5 38 41940.0 39 43283.0 40 44870.0 41 47312.5 42 49398.0 43 51536.5 44 54277.5 45 56080.0 46 57092.5 47 58619.5 48 60961.5 49 60225.0 50 57228.0 51 53978.0 52 50993.0 53 49536.0 54 47885.0 55 44605.0 56 40263.0 57 36381.5 58 32822.5 59 28675.0 60 24083.5 61 19868.0 62 15750.0 63 11911.0 64 8981.5 65 6964.5 66 5191.0 67 3883.5 68 2915.5 69 2035.5 70 1505.0 71 1106.0 72 834.5 73 630.5 74 443.0 75 359.5 76 267.0 77 159.5 78 98.5 79 74.0 80 48.5 81 28.5 82 19.5 83 11.0 84 10.0 85 10.0 86 6.0 87 5.5 88 5.5 89 4.5 90 5.5 91 4.5 92 1.5 93 1.5 94 3.0 95 3.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2812929240841367 2 0.2656766148094445 3 0.25291775787012144 4 0.24009244855090617 5 0.23623821051715235 6 0.19995521109595257 7 0.1927119016876911 8 0.20241394915196795 9 0.20440752054873718 10-11 0.20427461578895253 12-13 0.20098522298428334 14-15 0.19925746110708337 16-17 0.19909133015735261 18-19 0.19842680635842955 20-21 0.18659828273759882 22-23 0.17749430669235272 24-25 0.17932174713939117 26-27 0.1913496278998988 28-29 0.20563688957674486 30-31 0.19955649681659876 32-33 0.2052714014873372 34-35 0.19769583017961415 36-37 0.18443858039109884 38-39 0.18500342562018346 40-41 0.17938819951928348 42-43 0.19178156836919877 44-45 0.1870966755867911 46-47 0.18825959223490651 48-49 0.19796163969918334 50-51 0.2053378538672295 52-53 0.21653507987908324 54-55 0.1935757826262911 56-57 0.16722741399899124 58-59 0.16745999732861433 60-61 0.1735071638988143 62-63 0.1813817709160527 64-65 0.17118133060258353 66-67 0.17589944957493733 68-69 0.1785907709605758 70-71 0.171281009172422 72-73 0.1753678305357989 74-75 0.1772949495526758 76-77 0.19307738977709876 78-79 0.1971642111404757 80-81 0.1876282946259296 82-83 0.185767627988945 84-85 0.17819205668122196 86-87 0.18121563996632192 88-89 0.1877611993857142 90-91 0.18722958034657575 92-93 0.18390696135196039 94-95 0.18387373516201422 96-97 0.1722777948708066 98-99 0.18041821140761424 100-101 0.17167972345177585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1504837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.257377774542874 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42178112207662 48.03912671947563 2 12.716403360962996 15.579470492319542 3 3.9243208030223307 7.211808058177089 4 1.7191582167090023 4.212444973406113 5 0.9047012098458833 2.770981189230763 6 0.5258511428732457 1.932735726729692 7 0.350437346990335 1.5026811065626862 8 0.24096850266658193 1.1808878879690201 9 0.18420127479302786 1.0155318368894 >10 0.9303083440153449 10.092393003911207 >50 0.05785152802304252 2.419791491961796 >100 0.022387114546297875 2.33355390597866 >500 9.780200852166647E-4 0.4028678268560777 >1k 4.346755934296288E-4 0.5304781414765504 >5k 2.173377967148144E-4 0.7752476390558296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6456 0.42901656458473575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.3448878516410747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3266 0.21703347272827556 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1962 0.13037956934870687 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.9935713967692183E-4 14-15 0.0 0.0 0.0 0.0 1.9935713967692183E-4 16-17 0.0 0.0 0.0 0.0 1.9935713967692183E-4 18-19 0.0 0.0 0.0 0.0 1.9935713967692183E-4 20-21 0.0 0.0 0.0 0.0 1.9935713967692183E-4 22-23 0.0 0.0 0.0 0.0 1.9935713967692183E-4 24-25 0.0 0.0 0.0 6.645237989230728E-5 1.9935713967692183E-4 26-27 0.0 0.0 0.0 3.6548808940769E-4 1.9935713967692183E-4 28-29 0.0 0.0 0.0 0.0015616309274692208 1.9935713967692183E-4 30-31 0.0 0.0 0.0 0.004252952313107666 1.9935713967692183E-4 32-33 0.0 0.0 0.0 0.008140416536807641 1.9935713967692183E-4 34-35 0.0 0.0 0.0 0.012127559330346076 1.9935713967692183E-4 36-37 0.0 0.0 0.0 0.01608147593393836 1.9935713967692183E-4 38-39 0.0 0.0 0.0 0.019802809207907567 1.9935713967692183E-4 40-41 0.0 0.0 0.0 0.023789952001446003 1.9935713967692183E-4 42-43 0.0 0.0 0.0 0.02807613050449982 1.9935713967692183E-4 44-45 0.0 0.0 0.0 0.03143197568906134 1.9935713967692183E-4 46-47 0.0 0.0 0.0 0.03721333273969207 1.9935713967692183E-4 48-49 0.0 0.0 0.0 0.044157606438438184 1.9935713967692183E-4 50-51 0.0 0.0 0.0 0.04973960634939199 2.658095195692291E-4 52-53 0.0 0.0 0.0 0.062099749009361147 2.658095195692291E-4 54-55 0.0 0.0 0.0 0.08748455812822252 2.658095195692291E-4 56-57 0.0 0.0 0.0 0.12632597417527613 2.658095195692291E-4 58-59 0.0 0.0 0.0 0.16765935446829125 2.658095195692291E-4 60-61 0.0 0.0 0.0 0.2055372110069064 2.658095195692291E-4 62-63 0.0 0.0 0.0 0.24962836506545227 2.658095195692291E-4 64-65 0.0 0.0 0.0 0.3083058165103596 2.9903570951538277E-4 66-67 0.0 0.0 0.0 0.39333163658256676 3.322618994615364E-4 68-69 0.0 0.0 0.0 0.49447215877865835 3.322618994615364E-4 70-71 0.0 0.0 0.0 0.5879706572871348 3.322618994615364E-4 72-73 0.0 0.0 0.0 0.688147619974788 3.322618994615364E-4 74-75 0.0 0.0 0.0 0.7996879396240257 3.322618994615364E-4 76-77 0.0 0.0 0.0 0.9262464971289249 3.322618994615364E-4 78-79 0.0 0.0 0.0 1.0487182332704474 3.322618994615364E-4 80-81 0.0 0.0 0.0 1.1807258859265155 3.322618994615364E-4 82-83 0.0 0.0 0.0 1.328947919276307 3.322618994615364E-4 84-85 0.0 0.0 0.0 1.5238527495004441 3.322618994615364E-4 86-87 0.0 0.0 0.0 1.7547415434362659 3.322618994615364E-4 88-89 0.0 0.0 0.0 2.0185242654187796 3.322618994615364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5075 0.0 35.60396 1 GTATCAA 7425 0.0 32.59658 1 ATCAACG 9045 0.0 26.849207 3 TCAACGC 9125 0.0 26.559082 4 GTACATG 6315 0.0 26.429228 1 TATCAAC 9175 0.0 26.367823 2 CAACGCA 9205 0.0 26.173386 5 AACGCAG 9390 0.0 25.698912 6 TACATGG 6435 0.0 25.408115 2 ACATGGG 6560 0.0 24.197014 3 GAGTACT 5810 0.0 22.850374 12-13 CTTAGCG 125 2.2484297E-4 22.811844 3 ACGCAGA 10575 0.0 22.684416 7 CGCAGAG 10630 0.0 22.477673 8 CATGGGG 3265 0.0 21.83151 4 GTATAGG 350 0.0 21.737152 1 AACCGCT 175 4.048754E-6 21.715435 7 GTACTTT 6190 0.0 21.486332 14-15 GCAGAGT 11240 0.0 20.792223 9 AGTACTT 6040 0.0 20.446741 12-13 >>END_MODULE