FastQCFastQC Report
Thu 26 May 2016
SRR937793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504837
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.48290944467739694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.4129350886507974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40550.269464400463306No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18370.12207302186216845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.10738704590596854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.034.648921
GTATCAA85400.031.9394551
CCTATCG856.405042E-427.9434628
TATCAAC105850.025.7136672
ATCAACG105700.025.7052213
TCAACGC106150.025.6409974
CAACGCA108050.025.2340765
GTACATG61050.024.9079421
AACGCAG109850.024.8181176
ACATGGG59700.023.949283
TACATGG62700.023.9397622
GAGTACT70600.023.011812-13
GTACTTT72300.022.23705914-15
ACGCAGA123550.022.0313457
AGTACTT72600.021.95255512-13
CGCAGAG123700.021.9278268
AGAGTAC104450.020.78437410-11
GCAGAGT131100.020.3639859
CATGGGG28100.020.2850114
GCGTGCC1200.00476191619.7932879
Produced by FastQC (version 0.11.5)