##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937793_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1504837 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23381203412728 33.0 31.0 34.0 31.0 34.0 2 32.43740418397474 34.0 31.0 34.0 31.0 34.0 3 32.50511583646601 34.0 31.0 34.0 31.0 34.0 4 36.10327098549544 37.0 35.0 37.0 35.0 37.0 5 36.0261024948217 37.0 35.0 37.0 35.0 37.0 6 36.07375682549007 37.0 35.0 37.0 35.0 37.0 7 36.030053753330094 37.0 35.0 37.0 35.0 37.0 8 35.99762166932366 37.0 35.0 37.0 35.0 37.0 9 37.688308434734125 39.0 37.0 39.0 35.0 39.0 10-11 37.6053306770102 39.0 37.0 39.0 35.0 39.0 12-13 37.49878026656708 39.0 37.0 39.0 35.0 39.0 14-15 38.72416381309072 40.0 38.0 41.0 35.0 41.0 16-17 38.69131175004336 40.0 38.0 41.0 34.5 41.0 18-19 38.664691591182304 40.0 38.0 41.0 34.0 41.0 20-21 38.55626954945951 40.0 38.0 41.0 34.0 41.0 22-23 38.50224010972617 40.0 38.0 41.0 34.0 41.0 24-25 38.51941406278554 40.0 38.0 41.0 34.0 41.0 26-27 38.34340995071227 40.0 38.0 41.0 34.0 41.0 28-29 38.30779978163748 40.0 38.0 41.0 34.0 41.0 30-31 38.31811119742537 40.0 38.0 41.0 34.0 41.0 32-33 38.19097251064401 40.0 38.0 41.0 34.0 41.0 34-35 38.04116758160518 40.0 37.0 41.0 33.0 41.0 36-37 37.8719004118054 40.0 37.0 41.0 33.0 41.0 38-39 37.71901541495856 40.0 37.0 41.0 33.0 41.0 40-41 37.540686798636656 40.0 37.0 41.0 33.0 41.0 42-43 37.31535176235034 40.0 37.0 41.0 32.5 41.0 44-45 37.000461511778354 40.0 36.0 41.0 31.0 41.0 46-47 36.65452504158257 39.5 36.0 41.0 30.5 41.0 48-49 36.31201884323684 39.0 36.0 41.0 30.0 41.0 50-51 36.054309868776485 39.0 35.0 41.0 29.5 41.0 52-53 35.78120985860927 39.0 35.0 40.0 28.5 41.0 54-55 35.54955852361418 39.0 35.0 40.0 28.0 41.0 56-57 35.26457516661273 38.0 35.0 40.0 27.5 41.0 58-59 35.00337843899372 38.0 34.0 40.0 27.0 41.0 60-61 34.65131904651467 38.0 34.0 40.0 26.0 41.0 62-63 34.23723566073934 37.0 34.0 40.0 26.0 41.0 64-65 33.84153532907551 37.0 33.0 39.5 25.5 41.0 66-67 33.407474696595045 36.0 33.0 39.0 23.5 40.0 68-69 32.93406030021856 35.5 32.0 39.0 22.5 40.0 70-71 32.50724364167016 35.0 32.0 38.0 22.0 40.0 72-73 32.03033883403984 35.0 31.0 37.5 20.5 39.5 74-75 31.548381319704394 35.0 31.0 37.0 19.5 39.0 76-77 30.294586058157794 33.5 29.5 35.5 18.5 38.0 78-79 30.90226449774959 34.0 31.0 36.0 18.5 38.0 80-81 30.740567583067136 34.0 31.0 35.5 18.0 37.0 82-83 30.352102254264082 34.0 30.5 35.0 15.5 37.0 84-85 29.940513158568002 34.0 30.0 35.0 9.5 36.5 86-87 29.594907621224092 34.0 30.0 35.0 7.0 36.0 88-89 29.18794892735891 34.0 29.0 35.0 2.0 36.0 90-91 28.68497518335873 34.0 29.0 35.0 2.0 35.0 92-93 28.21581473608105 33.5 28.5 35.0 2.0 35.0 94-95 27.704164304838333 33.0 27.0 35.0 2.0 35.0 96-97 27.163231964657967 33.0 26.5 35.0 2.0 35.0 98-99 26.321694309749162 32.5 25.0 34.0 2.0 35.0 100-101 23.85010436346262 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15686741732743315 1101 2 0.18471401702358747 1101 3 0.18154540441639 1101 4 0.17522048277037783 1101 5 0.05969458102021008 1101 6 0.07951605991440402 1101 7 0.10651751479980476 1101 8 0.11811616218363241 1101 9 0.23412213620165545 1101 10-11 0.15961031510245505 1101 12-13 0.21935565024403303 1101 14-15 0.25322662464805745 1101 16-17 0.27258639353641456 1101 18-19 0.3026887798443241 1101 20-21 0.22925367405056107 1101 22-23 0.2608881032865398 1101 24-25 0.15567946134134303 1101 26-27 0.10006364538225654 1101 28-29 0.15917835228749766 1101 30-31 0.32963986052840966 1101 32-33 0.28693988972487006 1101 34-35 0.2670048256961053 1101 36-37 0.18914064423622534 1101 38-39 0.30216979618786866 1101 40-41 0.22464264917351073 1101 42-43 0.19451099462647647 1101 44-45 0.3112878089787401 1101 46-47 0.27799441698705607 1101 48-49 0.2141495006754326 1101 50-51 0.33219764593481216 1101 52-53 0.32195333776933666 1101 54-55 0.23906496243697006 1101 56-57 0.5311696109937358 1101 58-59 0.47146897297015045 1101 60-61 0.6370145387194626 1101 62-63 0.4592093106420805 1101 64-65 0.5867070235603151 1101 66-67 0.4854272121856056 1101 68-69 0.6475309191322651 1101 70-71 0.6030823675467047 1101 72-73 0.5294558345618867 1101 74-75 0.4721919451333747 1101 76-77 0.2715301720295997 1101 78-79 0.395553464945948 1101 80-81 0.47845323083972247 1101 82-83 0.4031034091286756 1101 84-85 0.5044450039270991 1101 86-87 0.7032679751763027 1101 88-89 0.774310988162739 1101 90-91 0.7016265435763032 1101 92-93 0.8545752520038405 1101 94-95 0.8626882599853758 1101 96-97 0.7817067805258411 1101 98-99 0.9958196361071892 1101 100-101 1.567827595207767 1102 1 0.20654733197133623 1102 2 0.15222164868832522 1102 3 0.21988229425367933 1102 4 0.13422362434886992 1102 5 0.12376574073687152 1102 6 0.1329332674660506 1102 7 0.0689245270401031 1102 8 0.14055622027319714 1102 9 0.08867372032779741 1102 10-11 0.20791467774731132 1102 12-13 0.1866165494467822 1102 14-15 0.2909422377004276 1102 16-17 0.26684930809140894 1102 18-19 0.38199066775516854 1102 20-21 0.3029429541548865 1102 22-23 0.3352116265964611 1102 24-25 0.3014190659766527 1102 26-27 0.25322485874285405 1102 28-29 0.3175239111031871 1102 30-31 0.18923093666722224 1102 32-33 0.24438002190826325 1102 34-35 0.08823382009461511 1102 36-37 0.27665953816349287 1102 38-39 0.2404840874062586 1102 40-41 0.28478498342561664 1102 42-43 0.2892840452177481 1102 44-45 0.33380788840732833 1102 46-47 0.26058458918111427 1102 48-49 0.26335336244597585 1102 50-51 0.164773740300717 1102 52-53 0.2620155024947479 1102 54-55 0.2720785808833739 1102 56-57 0.19182356315875637 1102 58-59 0.2281322313205223 1102 60-61 0.2735942933206914 1102 62-63 0.2407432752548644 1102 64-65 0.2228988969487702 1102 66-67 0.21161234303531273 1102 68-69 0.109540788430742 1102 70-71 0.12167436244904906 1102 72-73 0.15567504857451553 1102 74-75 0.17833469431092652 1102 76-77 0.1475519259787994 1102 78-79 0.1961802987683008 1102 80-81 0.08768828611372115 1102 82-83 0.3503278541658723 1102 84-85 0.3297654597523554 1102 86-87 0.32491485878971105 1102 88-89 0.5080529489819199 1102 90-91 0.48198760282173225 1102 92-93 0.6123521406556272 1102 94-95 0.7251263916352961 1102 96-97 0.8778205590149177 1102 98-99 1.1591302226339657 1102 100-101 1.673082700272822 1103 1 0.21311047896926993 1103 2 0.232611572773898 1103 3 0.14221148680026374 1103 4 0.1013009190348626 1103 5 0.1847836841461188 1103 6 0.2690340266102851 1103 7 0.1508879500904854 1103 8 0.24573910502130047 1103 9 0.21474342357141296 1103 10-11 0.3181924555250859 1103 12-13 0.30001199458135375 1103 14-15 0.343052313614848 1103 16-17 0.32327097820873263 1103 18-19 0.47233918397325425 1103 20-21 0.3363936097794564 1103 22-23 0.4371301911237211 1103 24-25 0.33268890724649225 1103 26-27 0.3531058111238039 1103 28-29 0.3276981692122263 1103 30-31 0.328014580559568 1103 32-33 0.3236640108661746 1103 34-35 0.3447572845528555 1103 36-37 0.279782381503729 1103 38-39 0.36427808326547506 1103 40-41 0.3532442656892414 1103 42-43 0.40569708869600163 1103 44-45 0.5229452245011856 1103 46-47 0.4500014283805669 1103 48-49 0.3630997392575708 1103 50-51 0.4173617320191383 1103 52-53 0.36720701388190946 1103 54-55 0.29430597908903167 1103 56-57 0.33801058869361356 1103 58-59 0.21837032655862032 1103 60-61 0.3595863568127555 1103 62-63 0.2726497624874398 1103 64-65 0.2322294697576055 1103 66-67 0.39464719465711795 1103 68-69 0.5062523136205286 1103 70-71 0.4156132858444934 1103 72-73 0.3072813287677505 1103 74-75 0.5364903188796006 1103 76-77 0.41455675689667615 1103 78-79 0.553426675579896 1103 80-81 0.45106302731455017 1103 82-83 0.5466149487138345 1103 84-85 0.43450148459707805 1103 86-87 0.6042057490588597 1103 88-89 0.5893728247583176 1103 90-91 0.5756366711447143 1103 92-93 0.7012341282332706 1103 94-95 0.6622519955000321 1103 96-97 0.7188730089804132 1103 98-99 0.9895356746698525 1103 100-101 1.6041368755661267 1104 1 0.12258307210383634 1104 2 0.18429297431445946 1104 3 0.05338031796909348 1104 4 0.06575959025409617 1104 5 0.14802007217124213 1104 6 0.12513792271418112 1104 7 0.150378225073716 1104 8 0.12222996939949127 1104 9 0.26767515537638076 1104 10-11 0.21019985344797476 1104 12-13 0.23673191003366156 1104 14-15 0.2061696032110163 1104 16-17 0.14843692337468184 1104 18-19 0.1961186283160785 1104 20-21 0.14489431095621086 1104 22-23 0.1900119259772879 1104 24-25 0.1501528830203327 1104 26-27 0.21128254831462812 1104 28-29 0.23459998414220706 1104 30-31 0.2804713413261908 1104 32-33 0.2916326306657737 1104 34-35 0.24378620555361152 1104 36-37 0.09906067754258885 1104 38-39 0.2896194242406196 1104 40-41 0.42550672936015843 1104 42-43 0.4558080575393646 1104 44-45 0.43898550659780966 1104 46-47 0.4337731496170747 1104 48-49 0.45733505011259723 1104 50-51 0.38374081459540577 1104 52-53 0.5789335346225144 1104 54-55 0.5752809196414361 1104 56-57 0.5538988475396209 1104 58-59 0.4417650382631848 1104 60-61 0.3866704776996883 1104 62-63 0.5666411857746354 1104 64-65 0.6898958722127375 1104 66-67 0.6064196475563364 1104 68-69 0.599958591693003 1104 70-71 0.6761246367783826 1104 72-73 0.7804581675783879 1104 74-75 0.7320086815810569 1104 76-77 0.7327471111433645 1104 78-79 0.7471740080596234 1104 80-81 0.6558948465125667 1104 82-83 0.49543941063688735 1104 84-85 0.4391272188002766 1104 86-87 0.4912022736610204 1104 88-89 0.4114326585082928 1104 90-91 0.522002339904752 1104 92-93 0.7268854096592001 1104 94-95 0.7226204689670972 1104 96-97 0.9186815572396654 1104 98-99 1.1826836534516367 1104 100-101 1.2498288049200283 1105 1 0.08524776854810057 1105 2 0.11594075772619306 1105 3 0.05331779452279761 1105 4 0.005788157304770891 1105 5 -0.007121732766172784 1105 6 0.06436513291800594 1105 7 0.08384006903985153 1105 8 0.02060559265257922 1105 9 0.11894420521373661 1105 10-11 0.1276667028122631 1105 12-13 0.10539964987272299 1105 14-15 0.1328837583431266 1105 16-17 0.20955359212545943 1105 18-19 0.2614494654081554 1105 20-21 0.1901788983102719 1105 22-23 0.144973469654758 1105 24-25 0.0880230702560354 1105 26-27 0.15125519650840857 1105 28-29 0.1369911977487206 1105 30-31 0.06540917893078557 1105 32-33 0.13328759987077632 1105 34-35 0.13534897469062912 1105 36-37 0.07513401138080411 1105 38-39 0.14916138275675195 1105 40-41 0.06945155373971801 1105 42-43 0.07613266870517776 1105 44-45 0.07851846526012451 1105 46-47 -0.006864360576003037 1105 48-49 -0.011084885532277156 1105 50-51 -0.0666833761662673 1105 52-53 -0.12661713842918232 1105 54-55 0.09677797751701434 1105 56-57 -0.08542012242623542 1105 58-59 -0.04546168584285937 1105 60-61 -0.05386444120475886 1105 62-63 -0.034092011709624614 1105 64-65 0.04319473252479611 1105 66-67 -2.889421084546484E-4 1105 68-69 0.023085133864626073 1105 70-71 -0.02665915362189253 1105 72-73 -0.08874190216607047 1105 74-75 0.062882319054264 1105 76-77 -0.005048928568708533 1105 78-79 0.1383189268407854 1105 80-81 0.21879255287076305 1105 82-83 0.040860052532526225 1105 84-85 0.18895990253555084 1105 86-87 0.38217195067017684 1105 88-89 0.4845665978200415 1105 90-91 0.5632572471146702 1105 92-93 0.48687286974546495 1105 94-95 0.5811897916180193 1105 96-97 0.6366708736558557 1105 98-99 0.7450829334633298 1105 100-101 1.0044154721033216 1106 1 -0.32644214462793997 1106 2 -0.274376092780642 1106 3 -0.2954181765300419 1106 4 -0.2315299372991788 1106 5 -0.1709548660510265 1106 6 -0.25333631005941015 1106 7 -0.18981420338236887 1106 8 -0.12325189689963878 1106 9 -0.46153551687950056 1106 10-11 -0.45255949068864254 1106 12-13 -0.4633438339496294 1106 14-15 -0.4571769351972037 1106 16-17 -0.3480888438517269 1106 18-19 -0.3557858792155031 1106 20-21 -0.3672680523376215 1106 22-23 -0.44543931650947854 1106 24-25 -0.41257598002476215 1106 26-27 -0.40114527572281844 1106 28-29 -0.550258559126668 1106 30-31 -0.47681455704027087 1106 32-33 -0.610898217422168 1106 34-35 -0.8105996055492568 1106 36-37 -0.6616770260384826 1106 38-39 -0.5822412272188018 1106 40-41 -0.8520903819702497 1106 42-43 -0.9254706395417855 1106 44-45 -0.804913687641168 1106 46-47 -0.7818821021016831 1106 48-49 -0.81932418147845 1106 50-51 -1.0186722888078705 1106 52-53 -1.1076112552516477 1106 54-55 -1.2944292347417203 1106 56-57 -1.32172300750495 1106 58-59 -1.2046349307460034 1106 60-61 -1.29806175763472 1106 62-63 -1.4058804670141285 1106 64-65 -1.8342759392000332 1106 66-67 -1.75864443409629 1106 68-69 -1.7765884001596746 1106 70-71 -1.891339499515933 1106 72-73 -1.6627328255139204 1106 74-75 -1.8038147564414047 1106 76-77 -1.7839517356723285 1106 78-79 -1.9133461481992988 1106 80-81 -1.8896590938056583 1106 82-83 -1.8898833831029656 1106 84-85 -1.7737358018249232 1106 86-87 -1.54821008351621 1106 88-89 -1.6763161854529862 1106 90-91 -1.8410896457089905 1106 92-93 -1.7966854451162426 1106 94-95 -2.0183407150449106 1106 96-97 -2.0043200453113705 1106 98-99 -2.069388347168182 1106 100-101 -1.317465085812902 1107 1 0.1675868085807366 1107 2 0.18375097472411284 1107 3 0.15949626192287525 1107 4 0.08988301464398063 1107 5 0.14665922764329054 1107 6 0.1157083457170387 1107 7 0.07778488563607056 1107 8 0.1272482589628865 1107 9 0.12421969520984533 1107 10-11 0.1496249691197562 1107 12-13 0.0804660393597274 1107 14-15 0.10486478245434228 1107 16-17 0.17576277502485738 1107 18-19 0.26449288207289356 1107 20-21 0.202937837404761 1107 22-23 0.20825799846775084 1107 24-25 0.07402275074249332 1107 26-27 0.11802377928501784 1107 28-29 0.08412401590833696 1107 30-31 0.2582790535267421 1107 32-33 0.2947939052393096 1107 34-35 0.4110793595235833 1107 36-37 0.40369741622957633 1107 38-39 0.4026235329622807 1107 40-41 0.4596968959256529 1107 42-43 0.4381636092706387 1107 44-45 0.28344495202794207 1107 46-47 0.22848624524376504 1107 48-49 0.4305147218076897 1107 50-51 0.3938594652736285 1107 52-53 0.34361686002148417 1107 54-55 0.3238541372858421 1107 56-57 0.39702597473603163 1107 58-59 0.5080912443876358 1107 60-61 0.489856163298775 1107 62-63 0.576813849354231 1107 64-65 0.6083172177372447 1107 66-67 0.8384268043826779 1107 68-69 0.719971283555715 1107 70-71 0.7588317166304961 1107 72-73 0.764552654147181 1107 74-75 0.8902204196367194 1107 76-77 0.7869648406561076 1107 78-79 0.8209698944744659 1107 80-81 0.9344672036916997 1107 82-83 0.9664410296209986 1107 84-85 1.0395996329384687 1107 86-87 1.0979488357749219 1107 88-89 1.0403385395078146 1107 90-91 1.0022801485990023 1107 92-93 1.0133015624628676 1107 94-95 1.1333923250187397 1107 96-97 1.3798156504635948 1107 98-99 1.3525806501472637 1107 100-101 1.8592126463175767 1108 1 0.06641807227686058 1108 2 0.06772954789187935 1108 3 0.09125910584788244 1108 4 0.0956252433591871 1108 5 -0.02232056295812157 1108 6 0.061938359514620345 1108 7 0.10824101744355374 1108 8 0.09149629077848687 1108 9 0.09561000443799372 1108 10-11 -0.00548622315359637 1108 12-13 0.08142984099919914 1108 14-15 0.035531936094471916 1108 16-17 -0.060135285197532085 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 21.0 5 31.0 6 105.0 7 176.0 8 420.0 9 824.0 10 1323.0 11 2137.0 12 3376.0 13 4904.0 14 6081.0 15 8417.0 16 11310.0 17 15583.0 18 14406.0 19 9147.0 20 7750.0 21 7813.0 22 8213.0 23 8821.0 24 10183.0 25 11935.0 26 14521.0 27 17939.0 28 22714.0 29 27856.0 30 34697.0 31 44004.0 32 56098.0 33 74196.0 34 101967.0 35 146753.0 36 227064.0 37 337063.0 38 247977.0 39 19003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82200350348853 19.709217099949143 15.202680467885244 22.266098928677085 2 15.6483393218003 20.54169321993013 40.08095228918481 23.729015169084757 3 19.55693540230603 24.566115798588154 29.870477666351903 26.006471132753912 4 13.19326491391753 17.02291811200689 36.821221714525706 32.96259525954988 5 13.72210869095682 37.65977171908489 33.928923889747296 14.689195700211002 6 31.95548753785294 36.62522917764515 16.876711564109602 14.542571720392308 7 28.57512142511116 33.14392189984696 20.093471917556517 18.18748475748536 8 26.49775357729774 35.52577455232693 19.002855458763975 18.973616411611356 9 26.333151032304496 17.083112656055107 20.246844010347964 36.33689230129244 10-11 25.159103610557153 27.11672426980464 26.608994861237463 21.115177258400745 12-13 26.000714398451585 24.278050522923053 27.658949501997824 22.06228557662754 14-15 22.705382709223656 25.722021720624895 26.32308349675081 25.24951207340064 16-17 21.818442794801033 29.194623736657192 26.793865382097863 22.193068086443915 18-19 21.748867153053787 27.49520379948127 29.557752766578705 21.198176280886237 20-21 22.4793938723372 26.413854308605316 29.96740831116308 21.139343507894406 22-23 22.28328911973291 26.28178461685681 29.80434152746914 21.630584735941138 24-25 22.149774360944075 26.44133550676917 29.9690265457322 21.43986358655456 26-27 21.87274474388265 26.669304859458087 29.969836883928817 21.488113512730443 28-29 21.664058372623877 26.88074613822962 29.957403401747058 21.497792087399446 30-31 21.92906006732293 26.574282322365256 29.84912314394288 21.64753446636894 32-33 21.81847602099098 26.6842521814655 29.824260036136803 21.673011761406716 34-35 22.20805309810963 26.641656205954533 29.738038073226537 21.412252622709303 36-37 22.20516236856926 26.467862702327334 29.732611436821045 21.594363492282366 38-39 21.947725899881515 26.832108726725885 29.67364571711089 21.54651965628171 40-41 22.114502842834703 26.787138266229675 29.536474406513403 21.561884484422222 42-43 22.020226726503182 26.791306867728693 29.279070602122637 21.90939580364549 44-45 22.071858945520344 26.939794808341368 29.210273272121835 21.778072974016453 46-47 22.28899209681846 27.288005278977057 28.627386221896455 21.795616402308024 48-49 22.396046880824965 27.30292383826288 28.415635713369618 21.88539356754253 50-51 22.45508982035928 27.558599369898534 28.206443621468637 21.779867188273546 52-53 22.571887377798188 27.747466252978313 27.776306595434118 21.904339773789378 54-55 22.359763881403765 27.65226399935674 27.972830279957233 22.01514183928226 56-57 22.427312725564295 27.802280246963623 27.93545081626781 21.834956211204272 58-59 22.537125283336334 27.427920764840312 28.02961383857521 22.005340113248145 60-61 22.425452058927313 27.571889845876996 28.299211143798296 21.703446951397392 62-63 22.249552609352374 27.673262951402712 28.310574500759884 21.766609938485033 64-65 22.258257871118268 27.6538920826641 28.141154158224445 21.946695887993183 66-67 22.1932009912037 27.692567367761423 28.019346945881846 22.09488469515303 68-69 22.249120668883073 27.491316335257572 28.111782206312046 22.147780789547305 70-71 22.4286085469722 27.579398964804824 27.965719875308753 22.026272612914223 72-73 22.464227022594475 27.459950811948403 27.898602971617525 22.177219193839598 74-75 22.21755578843423 27.96253016107392 27.665587701525148 22.1543263489667 76-77 22.250283585531193 27.711074355561433 27.815969437221437 22.222672621685938 78-79 22.39823980936141 27.65781277307775 27.7584216762347 22.18552574132614 80-81 22.255500097352737 27.965088577699778 27.590197476537327 22.189213848410162 82-83 22.306864207703835 27.967551259772666 27.59717842819977 22.12840610432373 84-85 22.29974638423934 27.837893244809813 27.544206659133174 22.318153711817672 86-87 22.165689705928283 27.785600699610658 27.629504059243626 22.419205535217436 88-89 22.26500278767734 27.804871889779427 27.481282025893833 22.448843296649404 90-91 22.287983385254414 27.75656074766355 27.539705088265837 22.4157507788162 92-93 22.345578880459964 27.5883162309016 27.607089421190977 22.459015467447454 94-95 22.24310672850282 27.694162224878845 27.55786839371972 22.50486265289862 96-97 22.084484897699884 27.771014402224292 27.5700956316199 22.57440506845592 98-99 22.1864965657296 27.782370390847618 27.26799403521456 22.763139008208224 100-101 22.018819031972285 27.716201873263298 27.538851305322776 22.726127789441644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 446.0 1 320.0 2 225.0 3 272.0 4 248.5 5 261.0 6 477.0 7 987.0 8 1613.0 9 1984.5 10 1950.0 11 1808.5 12 1929.5 13 2146.5 14 2357.5 15 2485.0 16 2561.0 17 2703.5 18 2841.0 19 3083.0 20 3291.5 21 3601.0 22 4267.0 23 5175.0 24 6212.5 25 7565.5 26 9339.0 27 11342.0 28 13273.5 29 15391.0 30 17898.5 31 20449.0 32 22978.0 33 25453.0 34 28530.5 35 31556.0 36 35648.5 37 39871.5 38 41890.5 39 43466.0 40 45411.5 41 47803.0 42 49666.5 43 51737.5 44 54358.0 45 56323.5 46 56738.0 47 58149.0 48 61163.5 49 60224.5 50 57222.0 51 53857.0 52 50700.5 53 49083.5 54 47873.0 55 44729.5 56 39852.5 57 36137.0 58 32778.5 59 28809.0 60 23995.0 61 19443.5 62 15514.5 63 11753.0 64 8658.0 65 6607.5 66 5084.5 67 3903.0 68 2767.0 69 1829.0 70 1342.5 71 954.0 72 724.5 73 585.0 74 438.5 75 334.5 76 227.5 77 145.0 78 91.0 79 59.0 80 36.5 81 16.0 82 11.5 83 8.5 84 5.5 85 3.0 86 1.0 87 1.0 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04133338029301512 2 0.0 3 0.0 4 6.645237989230728E-5 5 0.007442666547938415 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004950702301976892 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.6548808940769E-4 22-23 0.0013954999777384528 24-25 0.0 26-27 0.0012625952179538382 28-29 0.00146195235763076 30-31 3.322618994615364E-5 32-33 0.0 34-35 0.0 36-37 2.990357095153827E-4 38-39 0.0 40-41 6.645237989230728E-5 42-43 0.01947054730844603 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.322618994615364E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3290475978461455E-4 84-85 9.96785698384609E-5 86-87 0.0 88-89 0.0 90-91 0.009934630793899937 92-93 0.002093249966607679 94-95 0.0 96-97 0.0 98-99 3.322618994615364E-4 100-101 0.012625952179538382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1504837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.12054912067368 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92692071831124 45.479617950290894 2 13.431833646422831 15.881947617481169 3 4.220158809737384 7.484943186243684 4 1.8643676699477414 4.408897616405656 5 0.9827540608814106 2.9050479864940484 6 0.6023791010026979 2.1367789938058346 7 0.38668946074370597 1.600290528084156 8 0.2795090742943943 1.3219783965196614 9 0.1969217197922389 1.047790818711414 >10 1.021645966753559 10.73449052479139 >50 0.057882711613845214 2.340317650870404 >100 0.02713561924609089 2.779665938499527 >500 0.001125900783096839 0.4114978601169686 >1k 4.503603132387356E-4 0.5693873658105063 >5k 2.251801566193678E-4 0.8973475658746125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7267 0.48290944467739694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6214 0.4129350886507974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4055 0.269464400463306 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1837 0.12207302186216845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1616 0.10738704590596854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.967856983846091E-5 14-15 0.0 0.0 0.0 0.0 1.3290475978461455E-4 16-17 0.0 0.0 0.0 0.0 1.9935713967692183E-4 18-19 0.0 0.0 0.0 0.0 1.9935713967692183E-4 20-21 0.0 0.0 0.0 0.0 2.658095195692291E-4 22-23 0.0 0.0 0.0 9.967856983846091E-5 2.658095195692291E-4 24-25 0.0 0.0 0.0 2.3258332962307546E-4 2.658095195692291E-4 26-27 0.0 0.0 0.0 5.648452290846119E-4 2.658095195692291E-4 28-29 0.0 0.0 0.0 0.0017609880671461428 2.658095195692291E-4 30-31 0.0 0.0 0.0 0.004452309452784587 2.658095195692291E-4 32-33 0.0 0.0 0.0 0.008372999866430716 2.658095195692291E-4 34-35 0.0 0.0 0.0 0.012459821229807613 2.658095195692291E-4 36-37 0.0 0.0 0.0 0.016380511643453742 2.658095195692291E-4 38-39 0.0 0.0 0.0 0.020035392537530644 2.658095195692291E-4 40-41 0.0 0.0 0.0 0.023789952001446006 2.658095195692291E-4 42-43 0.0 0.0 0.0 0.027943225744715208 2.658095195692291E-4 44-45 0.0 0.0 0.0 0.03123261854938442 2.658095195692291E-4 46-47 0.0 0.0 0.0 0.03701397560001515 2.658095195692291E-4 48-49 0.0 0.0 0.0 0.04402470167865357 2.658095195692291E-4 50-51 0.0 0.0 0.0 0.04967315396949969 2.658095195692291E-4 52-53 0.0 0.0 0.0 0.062000070439522686 2.658095195692291E-4 54-55 0.0 0.0 0.0 0.08748455812822252 2.658095195692291E-4 56-57 0.0 0.0 0.0 0.12602693846576074 2.658095195692291E-4 58-59 0.0 0.0 0.0 0.1676925806582374 2.658095195692291E-4 60-61 0.0 0.0 0.0 0.20523817529739102 2.658095195692291E-4 62-63 0.0 0.0 0.0 0.24942900792577535 2.658095195692291E-4 64-65 0.0 0.0 0.0 0.30837226889025193 2.658095195692291E-4 66-67 0.0 0.0 0.0 0.39363067229208215 2.658095195692291E-4 68-69 0.0 0.0 0.0 0.49503700400774303 2.658095195692291E-4 70-71 0.0 0.0 0.0 0.588469050136327 2.658095195692291E-4 72-73 0.0 0.0 0.0 0.6890447271033341 2.9903570951538277E-4 74-75 0.0 0.0 0.0 0.8009837610319257 3.322618994615364E-4 76-77 0.0 0.0 0.0 0.928838139944725 3.322618994615364E-4 78-79 0.0 0.0 0.0 1.0521737570248473 3.322618994615364E-4 80-81 0.0 0.0 0.0 1.184679802530108 3.322618994615364E-4 82-83 0.0 0.0 0.0 1.3337324906285533 3.9871427935384365E-4 84-85 0.0 0.0 0.0 1.530730570819298 3.9871427935384365E-4 86-87 0.0 0.0 0.0 1.7658723170682273 4.319404692999973E-4 88-89 0.0 0.0 0.0 2.0368651222690564 4.651666592461509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5815 0.0 34.64892 1 GTATCAA 8540 0.0 31.939455 1 CCTATCG 85 6.405042E-4 27.943462 8 TATCAAC 10585 0.0 25.713667 2 ATCAACG 10570 0.0 25.705221 3 TCAACGC 10615 0.0 25.640997 4 CAACGCA 10805 0.0 25.234076 5 GTACATG 6105 0.0 24.907942 1 AACGCAG 10985 0.0 24.818117 6 ACATGGG 5970 0.0 23.94928 3 TACATGG 6270 0.0 23.939762 2 GAGTACT 7060 0.0 23.0118 12-13 GTACTTT 7230 0.0 22.237059 14-15 ACGCAGA 12355 0.0 22.031345 7 AGTACTT 7260 0.0 21.952555 12-13 CGCAGAG 12370 0.0 21.927826 8 AGAGTAC 10445 0.0 20.784374 10-11 GCAGAGT 13110 0.0 20.363985 9 CATGGGG 2810 0.0 20.285011 4 GCGTGCC 120 0.004761916 19.793287 9 >>END_MODULE