FastQCFastQC Report
Thu 26 May 2016
SRR937792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335081
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.4472387817667992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.36582050077860445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.2238066454394902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17080.1279323127211008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63500.037.3619161
GGTATCA40250.036.500171
ATCAACG76700.030.7894823
TCAACGC77600.030.5559964
TATCAAC78400.030.3642832
CAACGCA79050.029.996645
AACGCAG80700.029.4911336
CCGATTG650.00584219129.2330349
ACGCAGA91350.026.0538987
CGCAGAG91100.026.0211038
GTACATG51100.025.0284331
GCAGAGT95650.024.18649
GAGTACT52000.023.8861812-13
GTACCGT1201.7101168E-423.751846
TACATGG52300.023.7126542
CAATACG1050.002191587822.6275924
ACATGGG52700.022.450713
CGTTAAC652.9462334E-421.91942662-63
GTACTTT57100.021.708714-15
GTGTAGG5700.021.6870651
Produced by FastQC (version 0.11.5)