##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1335081 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.672397405101265 33.0 31.0 34.0 30.0 34.0 2 31.773871398064987 33.0 31.0 34.0 30.0 34.0 3 31.814985757418462 33.0 31.0 34.0 30.0 34.0 4 35.425631104030394 37.0 35.0 37.0 33.0 37.0 5 35.42934548540501 37.0 35.0 37.0 33.0 37.0 6 35.48738915466552 37.0 35.0 37.0 33.0 37.0 7 35.40801644244806 37.0 35.0 37.0 33.0 37.0 8 35.4028587029551 37.0 35.0 37.0 33.0 37.0 9 36.95430689224099 39.0 37.0 39.0 33.0 39.0 10-11 36.87157108819615 39.0 37.0 39.0 33.0 39.0 12-13 36.761985602371695 39.0 37.0 39.0 33.0 39.0 14-15 37.86732415486401 40.0 38.0 41.0 33.0 41.0 16-17 37.76750212159412 40.0 38.0 41.0 33.0 41.0 18-19 37.68804289777175 40.0 37.5 41.0 32.0 41.0 20-21 37.70095859352354 40.0 37.5 41.0 32.5 41.0 22-23 37.702524041612456 40.0 38.0 41.0 32.0 41.0 24-25 37.625064321940016 40.0 37.0 41.0 32.0 41.0 26-27 37.5090174304031 40.0 37.0 41.0 32.0 41.0 28-29 37.349806491141734 40.0 37.0 41.0 32.0 41.0 30-31 37.10248254600283 39.0 36.5 41.0 31.0 41.0 32-33 36.95306389649767 39.0 36.0 41.0 31.0 41.0 34-35 36.91625039978848 39.0 36.0 41.0 31.0 41.0 36-37 36.741640394852446 39.0 36.0 41.0 30.0 41.0 38-39 36.55684823617443 39.0 36.0 41.0 30.0 41.0 40-41 36.31321844891808 39.0 35.0 41.0 30.0 41.0 42-43 36.024216508211865 39.0 35.0 40.5 29.0 41.0 44-45 35.74547349561562 39.0 35.0 40.0 27.5 41.0 46-47 35.356063414878946 39.0 35.0 40.0 26.5 41.0 48-49 34.97250241745632 38.0 34.5 40.0 24.5 41.0 50-51 34.09319097492961 37.5 33.5 39.5 23.5 40.5 52-53 34.19709740457695 38.0 33.5 39.5 24.0 40.5 54-55 34.44015793798279 38.0 34.0 40.0 23.5 41.0 56-57 34.18473897838409 38.0 34.0 40.0 22.5 41.0 58-59 33.87799129790627 38.0 33.5 40.0 22.0 41.0 60-61 33.50408065128633 37.5 33.0 40.0 20.0 41.0 62-63 33.109491109528186 37.0 32.5 40.0 18.5 41.0 64-65 32.64953774340283 36.0 32.0 39.0 15.5 41.0 66-67 32.12009046642114 36.0 31.0 39.0 11.0 40.0 68-69 31.622349130876703 35.0 31.0 38.5 8.0 40.0 70-71 31.139671300842423 35.0 30.5 38.0 7.0 40.0 72-73 30.636124699550066 35.0 30.0 37.5 2.0 39.5 74-75 30.059794873869077 34.0 29.0 37.0 2.0 39.0 76-77 29.49785368827809 34.0 29.0 36.0 2.0 39.0 78-79 28.948990360884473 34.0 28.0 36.0 2.0 38.0 80-81 28.36464004805701 34.0 27.0 35.0 2.0 37.0 82-83 27.789115791476323 33.0 26.0 35.0 2.0 37.0 84-85 27.20781473184024 33.0 25.5 35.0 2.0 36.0 86-87 26.59097912411307 32.5 25.0 35.0 2.0 36.0 88-89 26.0017924006109 32.0 23.5 35.0 2.0 35.0 90-91 25.390119400995147 32.0 20.5 34.0 2.0 35.0 92-93 24.671458510757024 31.0 17.5 34.0 2.0 35.0 94-95 23.84499142748642 31.0 5.0 34.0 2.0 35.0 96-97 22.8976350498584 30.5 2.0 34.0 2.0 35.0 98-99 21.64833819071652 30.0 2.0 34.0 2.0 35.0 100-101 18.486800426341176 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28118157319795145 1101 2 0.12494632798119909 1101 3 0.13949955499977307 1101 4 0.07788058447645341 1101 5 0.14078108329270123 1101 6 0.15162574950858954 1101 7 0.19044775633951616 1101 8 0.19617839814748805 1101 9 0.30364903460286286 1101 10-11 0.36588235395449686 1101 12-13 0.433970536791513 1101 14-15 0.6069634336844771 1101 16-17 0.6059032848963071 1101 18-19 0.612811192773151 1101 20-21 0.47157340710668905 1101 22-23 0.4869558430671148 1101 24-25 0.444882209988144 1101 26-27 0.4845479037958498 1101 28-29 0.635597557605216 1101 30-31 0.7045872633963057 1101 32-33 0.6354408131546379 1101 34-35 0.9040509959217076 1101 36-37 0.8257916543737238 1101 38-39 0.8145541265116378 1101 40-41 0.809989395540093 1101 42-43 0.9048922171172151 1101 44-45 1.0104072844906185 1101 46-47 0.991428542738781 1101 48-49 1.1707956165547273 1101 50-51 1.1253743937934715 1101 52-53 1.156427271976959 1101 54-55 0.9796147837252462 1101 56-57 1.1095433295125332 1101 58-59 1.1901772764933654 1101 60-61 1.224977856820196 1101 62-63 1.2352419801498868 1101 64-65 1.3634607647871846 1101 66-67 1.4770095270663148 1101 68-69 1.5909503814784074 1101 70-71 1.5786857649521409 1101 72-73 1.6285791250898747 1101 74-75 1.5775532117855597 1101 76-77 1.5996897884196315 1101 78-79 1.799769440698956 1101 80-81 2.0875441889382493 1101 82-83 1.963810718656191 1101 84-85 2.192615403936916 1101 86-87 2.3534824561532943 1101 88-89 2.464363130902278 1101 90-91 2.6883309977582286 1101 92-93 2.6696837979979477 1101 94-95 2.7245791342059142 1101 96-97 2.972611966157295 1101 98-99 3.3407865750704993 1101 100-101 4.031966872239924 1102 1 0.22339774407265978 1102 2 0.3877182462409223 1102 3 0.22255168378361034 1102 4 0.2666106908894861 1102 5 0.21731275433656805 1102 6 0.3441183291962915 1102 7 0.3199897547839541 1102 8 0.3647110422898905 1102 9 0.5273500875722021 1102 10-11 0.45918340762140275 1102 12-13 0.5516007977144639 1102 14-15 0.6711946945620753 1102 16-17 0.642710780545265 1102 18-19 0.6202598435144822 1102 20-21 0.636050166095643 1102 22-23 0.47058995627440225 1102 24-25 0.49393082306114167 1102 26-27 0.6613634388430896 1102 28-29 0.6893383628704086 1102 30-31 0.7408482630719391 1102 32-33 0.7424273728851105 1102 34-35 0.7435756250804744 1102 36-37 0.8009748079105421 1102 38-39 0.8671528925250342 1102 40-41 0.88187072809216 1102 42-43 1.030646019700292 1102 44-45 1.200732191278938 1102 46-47 1.106262453304403 1102 48-49 1.2568104205573292 1102 50-51 1.1153922862503904 1102 52-53 1.1813555103198397 1102 54-55 1.385718840743941 1102 56-57 1.4316018863271864 1102 58-59 1.346109340962009 1102 60-61 1.3470393782719654 1102 62-63 1.486965393458668 1102 64-65 1.529398915481437 1102 66-67 1.475316850068758 1102 68-69 1.3193102744724428 1102 70-71 1.4297286632511401 1102 72-73 1.4182383400539713 1102 74-75 1.4385389797835266 1102 76-77 1.4688108694309605 1102 78-79 1.7933159052820038 1102 80-81 2.0675043320081485 1102 82-83 2.1904310672277596 1102 84-85 2.362233410445164 1102 86-87 2.3611299119714815 1102 88-89 2.4449737502324105 1102 90-91 2.6781394037251722 1102 92-93 2.6850556594063946 1102 94-95 2.90266270558217 1102 96-97 2.9043198064017197 1102 98-99 3.0016126886405132 1102 100-101 3.27888735544089 1103 1 0.02490769445804375 1103 2 -0.041087982132779644 1103 3 0.10273650280926105 1103 4 0.030809899984888034 1103 5 0.04397054163449354 1103 6 0.10726757582304458 1103 7 0.06815849940598184 1103 8 0.03744314166558382 1103 9 -0.041819279521035924 1103 10-11 0.11115929601597685 1103 12-13 0.12590143300125334 1103 14-15 0.2111118051971843 1103 16-17 0.3500570850801594 1103 18-19 0.1828305580748193 1103 20-21 0.246604257013054 1103 22-23 0.10624222371732372 1103 24-25 0.030920717057959735 1103 26-27 0.2096477525685856 1103 28-29 0.14977596928552117 1103 30-31 0.022651964944842007 1103 32-33 0.12457366277360649 1103 34-35 0.17090348432875402 1103 36-37 0.17919495217739723 1103 38-39 0.259552335962546 1103 40-41 0.33328204425261276 1103 42-43 0.3987416239550612 1103 44-45 0.4295118730862981 1103 46-47 0.5815709427132347 1103 48-49 0.5062856681727226 1103 50-51 0.5034792857560788 1103 52-53 0.48491000401273965 1103 54-55 0.39831541532148407 1103 56-57 0.6280322885534346 1103 58-59 0.7013728842878422 1103 60-61 0.6492139082146622 1103 62-63 0.855258266163311 1103 64-65 0.8038331989987597 1103 66-67 1.1151726289816324 1103 68-69 1.1546419234719139 1103 70-71 1.0862890978825135 1103 72-73 0.971272658356451 1103 74-75 1.07012824947833 1103 76-77 1.2809350982074363 1103 78-79 1.2513185507846494 1103 80-81 1.2254515958810153 1103 82-83 1.5237827085378797 1103 84-85 1.6485731424163852 1103 86-87 1.7633831662143535 1103 88-89 1.9671582122525102 1103 90-91 2.0443174762613765 1103 92-93 2.164139482478106 1103 94-95 2.0859479804764405 1103 96-97 2.1999396229557924 1103 98-99 2.541571907622224 1103 100-101 3.3452196726815124 1104 1 0.30524375018873684 1104 2 0.25210112613475033 1104 3 0.19600230048240164 1104 4 0.27079355182416975 1104 5 0.1841996454935355 1104 6 0.34611649999008165 1104 7 0.3548372741205199 1104 8 0.3631609567981968 1104 9 0.4685275907935065 1104 10-11 0.32782156184349986 1104 12-13 0.3594608546621103 1104 14-15 0.468765247150877 1104 16-17 0.3911095668090141 1104 18-19 0.5806444507969033 1104 20-21 0.5677099161860539 1104 22-23 0.45577439538219977 1104 24-25 0.45471831213330915 1104 26-27 0.39840999933856125 1104 28-29 0.449474263061056 1104 30-31 0.41039566619598133 1104 32-33 0.4426918792627461 1104 34-35 0.6656452086464171 1104 36-37 0.4170019107077181 1104 38-39 0.6788929907614829 1104 40-41 0.8938441730035578 1104 42-43 0.8742216789693771 1104 44-45 1.011099068251653 1104 46-47 0.7941874979812695 1104 48-49 0.9774371343409811 1104 50-51 0.9022915184051001 1104 52-53 0.974863232659672 1104 54-55 0.9408348689439165 1104 56-57 1.1121346206920322 1104 58-59 1.2678405564888493 1104 60-61 1.3756839224274628 1104 62-63 1.1929139123633945 1104 64-65 1.228289542291769 1104 66-67 1.4042698752809102 1104 68-69 1.4446732191784548 1104 70-71 1.6822383359250495 1104 72-73 1.6729915113217366 1104 74-75 1.8851981768989283 1104 76-77 1.8622791374430214 1104 78-79 2.041298643684449 1104 80-81 2.2092425782171965 1104 82-83 2.2652705289285358 1104 84-85 2.3913047547537367 1104 86-87 2.6564857118893244 1104 88-89 2.5425758136191448 1104 90-91 2.6957501216096134 1104 92-93 2.9606489664778017 1104 94-95 2.933288304638424 1104 96-97 3.1481159534825416 1104 98-99 3.543399824861954 1104 100-101 3.7815936414500975 1105 1 0.21648336215827868 1105 2 0.15232863585131184 1105 3 0.09683018306211011 1105 4 0.16145196073075851 1105 5 -0.02865886663504824 1105 6 0.04403415411211853 1105 7 -0.0432257334315338 1105 8 -0.02105134347250015 1105 9 0.20580126602338566 1105 10-11 0.12675195018994856 1105 12-13 0.1002419713556364 1105 14-15 0.27686448773187067 1105 16-17 0.19017951551400358 1105 18-19 0.1503824986371356 1105 20-21 0.24541765046313202 1105 22-23 0.07986725305169529 1105 24-25 0.14959460372492828 1105 26-27 0.2240438140234673 1105 28-29 0.3589812200132698 1105 30-31 0.27728404950772756 1105 32-33 0.2580298259875562 1105 34-35 0.45142795793281465 1105 36-37 0.39209433653007153 1105 38-39 0.2752818006539499 1105 40-41 0.2763211850426188 1105 42-43 0.3388470779013417 1105 44-45 0.4730385885853323 1105 46-47 0.382176354218295 1105 48-49 0.46872119496810427 1105 50-51 0.15796082881893625 1105 52-53 0.17895050791482703 1105 54-55 0.37504664257174625 1105 56-57 0.5835379132632141 1105 58-59 0.5332185280712025 1105 60-61 0.5958970021295897 1105 62-63 0.6908494723427552 1105 64-65 0.4808779919605115 1105 66-67 0.6317321939840994 1105 68-69 0.7654382206003945 1105 70-71 0.7365167825392618 1105 72-73 0.8859211202367483 1105 74-75 1.130247733992281 1105 76-77 0.9584994216195106 1105 78-79 1.0345075839736815 1105 80-81 1.1949995220033394 1105 82-83 1.1470207738174665 1105 84-85 1.1747334104451674 1105 86-87 1.3411082669498384 1105 88-89 1.4369470547057155 1105 90-91 1.4651463782321485 1105 92-93 1.606442143292874 1105 94-95 1.706234134153597 1105 96-97 1.4656473677292858 1105 98-99 1.6095672340950564 1105 100-101 2.100947240000778 1106 1 -0.07411471290581773 1106 2 -0.023689520749368143 1106 3 -0.006532150397312364 1106 4 -0.08342398317431332 1106 5 -0.11890109474136068 1106 6 -0.0950235519358813 1106 7 -0.10538267034575455 1106 8 -0.059355326436673295 1106 9 -0.03869501439523049 1106 10-11 -0.15369608074352215 1106 12-13 -0.32771162844262136 1106 14-15 -0.3364727683238371 1106 16-17 -0.37711058381493245 1106 18-19 -0.1498549661634314 1106 20-21 0.009044207293570139 1106 22-23 -0.033417466543703256 1106 24-25 -0.20780673280262363 1106 26-27 -0.18689707895625673 1106 28-29 -0.0044569060462222865 1106 30-31 0.17663960226133213 1106 32-33 0.17445272347841723 1106 34-35 0.11092935190022502 1106 36-37 -0.2744483440902812 1106 38-39 -0.2680117450667865 1106 40-41 0.04006472305799491 1106 42-43 0.10315590822958853 1106 44-45 -0.07005812053079552 1106 46-47 0.231187968278455 1106 48-49 0.22156937160269052 1106 50-51 0.2240608022004622 1106 52-53 0.3462833368981464 1106 54-55 0.21549551408858036 1106 56-57 0.035055166236773516 1106 58-59 0.1800103015619996 1106 60-61 0.5407582358257699 1106 62-63 0.2412662615944967 1106 64-65 0.32890962591447703 1106 66-67 0.5218847598648253 1106 68-69 0.4679284488187747 1106 70-71 0.3917599212775471 1106 72-73 0.36864222868601004 1106 74-75 0.5683695905607387 1106 76-77 0.8808729488474043 1106 78-79 0.6304447643768825 1106 80-81 0.5903153712026814 1106 82-83 0.7726522620703165 1106 84-85 0.9549846047740296 1106 86-87 1.1200180303256175 1106 88-89 1.2252846610044834 1106 90-91 1.4644437906363557 1106 92-93 1.7008445585690772 1106 94-95 1.748806179152826 1106 96-97 1.8309571927478459 1106 98-99 1.9215329382937725 1106 100-101 2.357387509547845 1107 1 0.25829617221926426 1107 2 0.256331384927055 1107 3 0.2619445698918277 1107 4 0.3038891059051281 1107 5 0.32570594940859365 1107 6 0.29643169574323736 1107 7 0.4153050909752025 1107 8 0.36548345182142583 1107 9 0.37396886484791736 1107 10-11 0.48741014843938757 1107 12-13 0.5248971519414809 1107 14-15 0.6546892683851198 1107 16-17 0.5298786444467041 1107 18-19 0.6760326392507849 1107 20-21 0.769476671328718 1107 22-23 0.7260547537100024 1107 24-25 0.5375816167119396 1107 26-27 0.6715520033199809 1107 28-29 0.6291773154830835 1107 30-31 0.5692792096598183 1107 32-33 0.6817015010278453 1107 34-35 0.8010549631255159 1107 36-37 0.8901687985643818 1107 38-39 0.9315887439134443 1107 40-41 0.8720707821608258 1107 42-43 0.7398072795002335 1107 44-45 0.837907296519738 1107 46-47 0.8870043526594387 1107 48-49 0.8547181562387891 1107 50-51 0.6803688243108681 1107 52-53 0.8538031795740721 1107 54-55 0.8489304230248678 1107 56-57 1.1485065516807538 1107 58-59 1.0930728059911772 1107 60-61 1.305635813601974 1107 62-63 1.3790452992247069 1107 64-65 1.4842201992260726 1107 66-67 1.5331268759444825 1107 68-69 1.5358311488400318 1107 70-71 1.6474009105766783 1107 72-73 1.6902858046196805 1107 74-75 1.5634496699314404 1107 76-77 1.7668909489891398 1107 78-79 1.972577674872241 1107 80-81 2.1715020340897944 1107 82-83 2.1030691354315216 1107 84-85 2.279327316133962 1107 86-87 2.4159830576092993 1107 88-89 2.307547611893863 1107 90-91 2.3178055297051685 1107 92-93 2.6527110102734213 1107 94-95 2.716176493092309 1107 96-97 2.7654019521554005 1107 98-99 2.90678734974583 1107 100-101 3.631460927448771 1108 1 0.030911777694726084 1108 2 -0.07892533639989097 1108 3 0.07495132323311182 1108 4 0.1266720284438989 1108 5 -0.032128465672521145 1108 6 0.08018913486543511 1108 7 0.1174091072587089 1108 8 -0.07723446475257134 1108 9 0.15063344215749908 1108 10-11 0.013601250474145843 1108 12-13 0.0045002246443601734 1108 14-15 0.008453228323382689 1108 16-17 -3.0666804697432326E-4 1108 18-19 0.11010836833531101 1108 20-21 0.060024942217232535 1108 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-2.0104930925311777 2316 88-89 -2.4766816597825994 2316 90-91 -2.4162639179345966 2316 92-93 -2.411240826395826 2316 94-95 -2.5274152950545705 2316 96-97 -2.920633575141917 2316 98-99 -3.039448506611226 2316 100-101 -3.181199855183568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5563.0 3 1213.0 4 1241.0 5 1280.0 6 1593.0 7 1830.0 8 2179.0 9 2842.0 10 3443.0 11 4450.0 12 5761.0 13 7316.0 14 8437.0 15 10130.0 16 12795.0 17 16169.0 18 13915.0 19 10720.0 20 10636.0 21 11244.0 22 12378.0 23 13830.0 24 15546.0 25 17570.0 26 20378.0 27 23671.0 28 27576.0 29 32567.0 30 39272.0 31 47928.0 32 60505.0 33 77003.0 34 100801.0 35 137986.0 36 189051.0 37 231748.0 38 143334.0 39 11178.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84188048634822 18.731392898594816 14.806125553564788 22.620601061492174 2 15.134333087787514 19.536586702830235 41.503776674949144 23.82530353443311 3 19.295237880682308 24.216407402818298 29.89317803785515 26.595176678644243 4 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30.213593646407904 21.774980573697817 24-25 21.82437812027948 26.088744444185973 30.300920072068937 21.785957363465613 26-27 21.68260418484144 26.332194537155335 30.223763130394975 21.761438147608246 28-29 21.476330760349253 26.483049336979114 30.31374495825327 21.726874944418366 30-31 21.659448389002 26.227451168745596 30.250124390523958 21.86297605172845 32-33 21.5073969481632 26.272505476510855 30.235668201865806 21.98442937346014 34-35 22.016786415971108 26.300181762783808 30.001103184525995 21.681928636719082 36-37 21.912302463674475 26.170397335140322 30.040792344006427 21.87650785717877 38-39 21.637427558783042 26.43885411660452 30.063100347147582 21.86061797746486 40-41 21.884628702777267 26.458085493595462 29.91653076514153 21.740755038485737 42-43 21.759935467532102 26.32587020344611 29.671803626199434 22.242390702822355 44-45 21.787261152232247 26.52392079528878 29.62057497553743 22.06824307694155 46-47 21.96644767969995 26.816116610278367 29.121464412335364 22.095971297686322 48-49 22.053962577790955 26.760162777040858 28.99251218107351 22.19336246409468 50-51 22.044092838403305 27.053615918928493 28.762779989073593 22.139511253594605 52-53 22.12312271519307 27.161462066294767 28.503208017153455 22.21220720135871 54-55 22.032742769792783 26.954439132232554 28.706205832493474 22.30661226548119 56-57 22.08317890096171 27.218949339679693 28.571514317568976 22.126357441789622 58-59 22.204680580201998 26.857541737042663 28.6991890960253 22.23858858673004 60-61 22.159347331624907 26.94230328431268 28.963228318010394 21.935121066052023 62-63 21.979631699045203 27.01262543565574 28.917450575041197 22.090292290257853 64-65 21.922529930151413 27.092128181667103 28.78891726249361 22.19642462568788 66-67 22.00164839514065 27.080573659515995 28.60370727593512 22.31407066940824 68-69 21.862113982173405 26.940442963896643 28.816437081720238 22.381005972209717 70-71 22.039482322540756 26.929154358609214 28.67866797619146 22.35269534265857 72-73 22.072845925476265 26.89148522592847 28.604975127364625 22.43069372123064 74-75 21.845082745900225 27.29676715437247 28.456688032873238 22.401462066854062 76-77 21.8845720357361 27.07401298757821 28.459064516177445 22.582350460508245 78-79 22.052456467615027 26.920707503492945 28.562542114196443 22.464293914695585 80-81 21.918848953252795 27.252157274705485 28.348315449838672 22.480678322203048 82-83 22.032980162908814 27.209478467458187 28.433301583883637 22.324239785749363 84-85 22.006957123248668 27.011276721771715 28.305318151654486 22.676448003325135 86-87 21.88419460036982 27.035402927716447 28.45784500232028 22.622557469593456 88-89 22.02723428266148 26.982506610481327 28.274664373632508 22.715594733224684 90-91 22.036782619983207 26.851380117336422 28.386890554785634 22.724946707894738 92-93 22.035267261769665 26.686374217121934 28.554252347930802 22.7241061731776 94-95 21.912429663604545 26.836868332368567 28.462854092331767 22.787847911695113 96-97 21.757093146496338 26.781507639494638 28.56771434199049 22.893684872018532 98-99 21.923807351393044 26.810592116765097 28.20078680780085 23.06481372404101 100-101 22.249351058562276 25.99460590874308 28.91844344081504 22.837599591879602 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1678.0 1 981.5 2 297.5 3 319.5 4 273.5 5 261.0 6 416.0 7 869.0 8 1523.0 9 1863.5 10 1732.0 11 1552.0 12 1535.5 13 1639.0 14 1835.5 15 1946.0 16 2040.0 17 2194.5 18 2357.0 19 2489.5 20 2739.0 21 3134.0 22 3626.5 23 4497.5 24 5604.0 25 6850.5 26 8493.5 27 10448.5 28 12195.5 29 14016.0 30 16112.5 31 18366.5 32 20771.0 33 23048.0 34 25938.5 35 28889.0 36 32335.5 37 35770.5 38 37392.5 39 38568.5 40 40274.5 41 42561.5 42 44358.5 43 45927.0 44 47995.0 45 49714.5 46 50417.0 47 51641.0 48 54043.0 49 53591.0 50 50381.5 51 47237.0 52 44882.0 53 43429.0 54 41902.5 55 39008.0 56 35195.5 57 31559.0 58 28322.5 59 24879.0 60 20969.0 61 17284.0 62 13544.5 63 10186.5 64 7795.5 65 5992.0 66 4445.0 67 3265.5 68 2320.0 69 1640.0 70 1227.0 71 888.5 72 667.0 73 504.0 74 378.0 75 284.5 76 202.0 77 131.5 78 82.0 79 52.5 80 34.0 81 23.5 82 18.0 83 12.0 84 7.5 85 7.0 86 7.0 87 5.5 88 5.5 89 3.5 90 1.0 91 1.5 92 3.0 93 4.0 94 3.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2948135731090473 2 0.2512207124511546 3 0.25661364366656403 4 0.2519697306755171 5 0.2524191416101345 6 0.19249768366114114 7 0.18605612693162438 8 0.18965141440856398 9 0.19264748730601364 10-11 0.19586826567077206 12-13 0.19845237854482237 14-15 0.1938084655537754 16-17 0.18680514515598678 18-19 0.1798392756694163 20-21 0.17894045380018142 22-23 0.18579397055309754 24-25 0.18485769777264452 26-27 0.18976376714221835 28-29 0.19335905461915795 30-31 0.19354630917524854 32-33 0.19264748730601364 34-35 0.19287219277332235 36-37 0.18560671599700693 38-39 0.176955555505621 40-41 0.1733602680286814 42-43 0.18115005756205055 44-45 0.18223613398737604 46-47 0.1876665161140036 48-49 0.19350885826403041 50-51 0.18942670894125524 52-53 0.1975910075868056 54-55 0.18815337795983914 56-57 0.16800478772449012 58-59 0.1544850087747485 60-61 0.17043909695366796 62-63 0.16302381653248005 64-65 0.1699896860190505 66-67 0.1699896860190505 68-69 0.16785498407961766 70-71 0.17092595879950356 72-73 0.179127708356272 74-75 0.18328475950148343 76-77 0.18148711576301363 78-79 0.17938986473479887 80-81 0.17834123922069148 82-83 0.1684916495703257 84-85 0.16497126391582234 86-87 0.16983988237417805 88-89 0.17594438090273173 90-91 0.17467104992131563 92-93 0.16916576597225186 94-95 0.17148772246777536 96-97 0.17740496644023845 98-99 0.17631889001491294 100-101 0.16036480183599347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1335081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.68123815791349 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3813887944788 49.7572373633265 2 12.187609848717294 15.278689510063614 3 3.7833169580935695 7.114289738314077 4 1.6127040971168367 4.043451583584932 5 0.8533748916532391 2.6745297410850153 6 0.5102261364296108 1.9188963583161898 7 0.32628181637258513 1.4316223767052625 8 0.2406349164978601 1.2066635608089598 9 0.16505477350983047 0.931125381072314 >10 0.8670896312779122 9.66761169885822 >50 0.05174085815357447 2.2056274264063274 >100 0.01902204292241749 2.0618099416973084 >500 8.374341104532945E-4 0.3603160146360495 >1k 5.981672217523533E-4 0.9003776041071897 >5k 1.1963344435047064E-4 0.4477517010180753 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5971 0.4472387817667992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4884 0.36582050077860445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2988 0.2238066454394902 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1708 0.1279323127211008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.49018224362417E-5 7 0.0 0.0 0.0 0.0 7.49018224362417E-5 8 0.0 0.0 0.0 0.0 7.49018224362417E-5 9 0.0 0.0 0.0 0.0 7.49018224362417E-5 10-11 0.0 0.0 0.0 0.0 7.49018224362417E-5 12-13 0.0 0.0 0.0 3.745091121812085E-5 7.49018224362417E-5 14-15 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 16-17 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 18-19 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 20-21 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 22-23 0.0 0.0 0.0 1.498036448724834E-4 7.49018224362417E-5 24-25 0.0 0.0 0.0 3.3705820096308764E-4 7.49018224362417E-5 26-27 0.0 0.0 0.0 4.86861845835571E-4 7.49018224362417E-5 28-29 0.0 0.0 0.0 0.0013107818926342296 7.49018224362417E-5 30-31 0.0 0.0 0.0 0.002921171075013426 7.49018224362417E-5 32-33 0.0 0.0 0.0 0.0057674403275906104 7.49018224362417E-5 34-35 0.0 0.0 0.0 0.009437629626966454 7.49018224362417E-5 36-37 0.0 0.0 0.0 0.013369975304869143 7.49018224362417E-5 38-39 0.0 0.0 0.0 0.016590753669627534 7.49018224362417E-5 40-41 0.0 0.0 0.0 0.019961335679258414 7.49018224362417E-5 42-43 0.0 0.0 0.0 0.023257015866453047 7.49018224362417E-5 44-45 0.0 0.0 0.0 0.027226812455573856 7.49018224362417E-5 46-47 0.0 0.0 0.0 0.032020529091493326 7.49018224362417E-5 48-49 0.0 0.0 0.0 0.03831228217613763 7.49018224362417E-5 50-51 0.0 0.0 0.0 0.04273148969987589 7.49018224362417E-5 52-53 0.0 0.0 0.0 0.05419146853262087 7.49018224362417E-5 54-55 0.0 0.0 0.0 0.07872181538049003 7.49018224362417E-5 56-57 0.0 0.0 0.0 0.11939350496336927 7.49018224362417E-5 58-59 0.0 0.0 0.0 0.16216244557446327 7.49018224362417E-5 60-61 0.0 0.0 0.0 0.19571846202589954 7.49018224362417E-5 62-63 0.0 0.0 0.0 0.2378881880575036 7.49018224362417E-5 64-65 0.0 0.0 0.0 0.29219200932377887 7.49018224362417E-5 66-67 0.0 0.0 0.0 0.37480871947095346 7.49018224362417E-5 68-69 0.0 0.0 0.0 0.4798959763490006 7.49018224362417E-5 70-71 0.0 0.0 0.0 0.5725120797914134 7.49018224362417E-5 72-73 0.0 0.0 0.0 0.6793595294967122 7.49018224362417E-5 74-75 0.0 0.0 0.0 0.7974797034786654 1.1235273365436255E-4 76-77 0.0 0.0 0.0 0.9313292601722293 1.498036448724834E-4 78-79 0.0 0.0 0.0 1.052445507051632 1.498036448724834E-4 80-81 0.0 0.0 0.0 1.1858831037217965 1.498036448724834E-4 82-83 0.0 0.0 0.0 1.3395067415385284 1.498036448724834E-4 84-85 0.0 0.0 0.0 1.5512167426545656 1.498036448724834E-4 86-87 0.0 0.0 0.0 1.7929998254787538 1.498036448724834E-4 88-89 0.0 0.0 0.0 2.0847424238679153 1.498036448724834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6350 0.0 37.361916 1 GGTATCA 4025 0.0 36.50017 1 ATCAACG 7670 0.0 30.789482 3 TCAACGC 7760 0.0 30.555996 4 TATCAAC 7840 0.0 30.364283 2 CAACGCA 7905 0.0 29.99664 5 AACGCAG 8070 0.0 29.491133 6 CCGATTG 65 0.005842191 29.233034 9 ACGCAGA 9135 0.0 26.053898 7 CGCAGAG 9110 0.0 26.021103 8 GTACATG 5110 0.0 25.028433 1 GCAGAGT 9565 0.0 24.1864 9 GAGTACT 5200 0.0 23.88618 12-13 GTACCGT 120 1.7101168E-4 23.75184 6 TACATGG 5230 0.0 23.712654 2 CAATACG 105 0.0021915878 22.627592 4 ACATGGG 5270 0.0 22.45071 3 CGTTAAC 65 2.9462334E-4 21.919426 62-63 GTACTTT 5710 0.0 21.7087 14-15 GTGTAGG 570 0.0 21.687065 1 >>END_MODULE