FastQCFastQC Report
Thu 26 May 2016
SRR937792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335081
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63480.47547676882526224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.41218472886663804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.2805822268461614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18530.13879307697435586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.10606098056971824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.031.105951
GTATCAA82800.030.8195861
TCAACGC99450.025.6510964
ATCAACG99650.025.6472853
TATCAAC99950.025.6178322
GTATAGG3000.025.3443721
CAACGCA101300.025.182645
AACGCAG101950.025.0183356
TATACCG950.001225954225.0024645
GTACATG54850.023.4787791
GAGTACT61950.023.00210412-13
TACATGG55700.022.8568332
ACGCAGA113800.022.2887957
CGCAGAG113500.022.264018
ACATGGG55650.021.7676433
GTACTTT65500.021.42829114-15
GCAGAGT120800.020.6826619
AGAGTAC92300.020.53330410-11
AGTACTT63550.020.06856712-13
GGACCGA3800.019.9989746
Produced by FastQC (version 0.11.5)