##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1335081 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.233026310763165 33.0 31.0 34.0 31.0 34.0 2 32.451594322741464 34.0 31.0 34.0 31.0 34.0 3 32.56018024374551 34.0 31.0 34.0 31.0 34.0 4 36.17313930765249 37.0 35.0 37.0 35.0 37.0 5 36.01728284650894 37.0 35.0 37.0 35.0 37.0 6 36.114552600179316 37.0 35.0 37.0 35.0 37.0 7 36.07672343475789 37.0 35.0 37.0 35.0 37.0 8 36.07047737178493 37.0 35.0 37.0 35.0 37.0 9 37.74049964009674 39.0 37.0 39.0 35.0 39.0 10-11 37.69362945019815 39.0 37.0 39.0 35.0 39.0 12-13 37.61599670731589 39.0 37.0 39.0 35.0 39.0 14-15 38.858387243920035 40.0 38.0 41.0 35.0 41.0 16-17 38.83086419475672 40.0 38.0 41.0 35.0 41.0 18-19 38.81495579668949 40.0 38.0 41.0 35.0 41.0 20-21 38.73146011365603 40.0 38.0 41.0 34.5 41.0 22-23 38.58558918897056 40.0 38.0 41.0 34.0 41.0 24-25 38.52890161720525 40.0 38.0 41.0 34.0 41.0 26-27 38.45597420680843 40.0 38.0 41.0 34.0 41.0 28-29 38.34314397403603 40.0 38.0 41.0 34.0 41.0 30-31 38.41323597594453 40.0 38.0 41.0 34.0 41.0 32-33 38.33335243329805 40.0 38.0 41.0 34.0 41.0 34-35 38.200948107268395 40.0 38.0 41.0 34.0 41.0 36-37 38.045383763232344 40.0 37.5 41.0 33.5 41.0 38-39 37.91076346678591 40.0 37.0 41.0 33.0 41.0 40-41 37.73010439066993 40.0 37.0 41.0 33.0 41.0 42-43 37.52539359035144 40.0 37.0 41.0 33.0 41.0 44-45 37.2236193908834 40.0 37.0 41.0 32.0 41.0 46-47 36.86410038042636 40.0 36.0 41.0 31.0 41.0 48-49 36.52862897457158 39.5 36.0 41.0 30.5 41.0 50-51 36.27309728773011 39.0 36.0 41.0 30.0 41.0 52-53 36.04942883615301 39.0 35.0 40.5 29.5 41.0 54-55 35.83271202271622 39.0 35.0 40.0 29.0 41.0 56-57 35.546745103855116 38.5 35.0 40.0 28.0 41.0 58-59 35.28892778790201 38.0 35.0 40.0 28.0 41.0 60-61 34.934364656526455 38.0 34.0 40.0 27.0 41.0 62-63 34.568098115395244 37.5 34.0 40.0 26.5 41.0 64-65 34.16169393467513 37.0 34.0 39.5 26.0 41.0 66-67 33.727933361346615 36.0 33.0 39.0 25.5 40.5 68-69 33.284676734969636 36.0 33.0 39.0 25.0 40.0 70-71 32.87356272765473 35.0 32.0 38.5 24.0 40.0 72-73 32.41835064688959 35.0 32.0 37.5 22.5 39.5 74-75 31.92447012578263 35.0 31.0 37.0 21.5 39.0 76-77 30.708392224891224 34.0 30.0 35.5 20.0 38.0 78-79 31.263251068661752 34.0 31.0 36.0 20.5 38.5 80-81 31.06256811384478 34.0 31.0 36.0 20.0 37.5 82-83 30.708536785408526 34.0 31.0 35.0 18.5 37.0 84-85 30.30793674690899 34.0 30.5 35.0 17.5 36.5 86-87 29.89256681804325 34.0 30.0 35.0 11.5 36.0 88-89 29.464081580068925 34.0 29.5 35.0 7.0 36.0 90-91 28.9435955571235 34.0 29.0 35.0 2.0 35.0 92-93 28.498277258083967 34.0 29.0 35.0 2.0 35.0 94-95 28.022501630987186 33.0 28.5 35.0 2.0 35.0 96-97 27.46825510961507 33.0 27.0 35.0 2.0 35.0 98-99 26.647093696936743 33.0 25.5 34.5 2.0 35.0 100-101 24.195170180685665 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24637255091810317 1101 2 0.20379431038092122 1101 3 0.24275798948355032 1101 4 0.14863485390164044 1101 5 0.10756685667477939 1101 6 0.10642975564405788 1101 7 0.09940155906060255 1101 8 0.1368647789858457 1101 9 0.19351418601490167 1101 10-11 0.2865912031488733 1101 12-13 0.2902566584366042 1101 14-15 0.2963036924425708 1101 16-17 0.22808662644346356 1101 18-19 0.34842779583764383 1101 20-21 0.36407174339181125 1101 22-23 0.39740481831417895 1101 24-25 0.26278875480011976 1101 26-27 0.32446711616160684 1101 28-29 0.4024162529790303 1101 30-31 0.4166786363479531 1101 32-33 0.5567158163023578 1101 34-35 0.47985840235192256 1101 36-37 0.41510050841642254 1101 38-39 0.5776656814838148 1101 40-41 0.27045661561525947 1101 42-43 0.29991348963891085 1101 44-45 0.4224183529626444 1101 46-47 0.36137674806843023 1101 48-49 0.44432468041112827 1101 50-51 0.5762008091702953 1101 52-53 0.5863852113725372 1101 54-55 0.659645358366781 1101 56-57 0.7283457452760942 1101 58-59 0.7721801721391657 1101 60-61 0.7105476489461395 1101 62-63 0.7902365638413045 1101 64-65 0.6657866775646326 1101 66-67 0.8830263281668707 1101 68-69 0.7596822019121845 1101 70-71 0.8064763227185381 1101 72-73 0.6398355129888742 1101 74-75 0.6869140480844038 1101 76-77 0.5265768508735498 1101 78-79 0.7631683596271124 1101 80-81 0.7713814118819222 1101 82-83 0.7815885759199688 1101 84-85 0.9608875654298252 1101 86-87 1.1427371317031039 1101 88-89 1.1553614357871602 1101 90-91 1.1984392717256078 1101 92-93 1.5204542494030413 1101 94-95 1.4062808055676577 1101 96-97 1.5439984268332019 1101 98-99 1.8690276752124149 1101 100-101 2.5362132797703687 1102 1 0.2756326472513706 1102 2 0.3533061916001188 1102 3 0.32864614244487456 1102 4 0.20388784208145694 1102 5 0.11250166953642093 1102 6 0.10440987862418183 1102 7 0.0688487937517408 1102 8 0.17717948393469385 1102 9 0.2719497644504827 1102 10-11 0.1935066115886741 1102 12-13 0.2882021213210919 1102 14-15 0.3257998698046052 1102 16-17 0.3015876100054271 1102 18-19 0.359123814155609 1102 20-21 0.3199971810245543 1102 22-23 0.41016322131648053 1102 24-25 0.25273710907773506 1102 26-27 0.332264983715568 1102 28-29 0.3121899203746423 1102 30-31 0.4384602175807544 1102 32-33 0.42227824284039883 1102 34-35 0.44393070636325405 1102 36-37 0.4026078328139846 1102 38-39 0.36980734405230464 1102 40-41 0.43762334680637593 1102 42-43 0.4764482561127039 1102 44-45 0.49319710209505274 1102 46-47 0.47140990840647135 1102 48-49 0.450686660858473 1102 50-51 0.6373789297996311 1102 52-53 0.5145546700298027 1102 54-55 0.4352646187421314 1102 56-57 0.5158553803467001 1102 58-59 0.4894943940265577 1102 60-61 0.4651812023601778 1102 62-63 0.6110318800634431 1102 64-65 0.6431645345156554 1102 66-67 0.6570091524545703 1102 68-69 0.6279975991909907 1102 70-71 0.55351059566744 1102 72-73 0.6265785713782677 1102 74-75 0.5997181849739022 1102 76-77 0.6094282126883286 1102 78-79 0.8781093023119588 1102 80-81 0.7666501998336379 1102 82-83 0.7072725351405147 1102 84-85 0.9367628309392408 1102 86-87 1.0590218833537115 1102 88-89 0.9839393604016742 1102 90-91 1.142498095357599 1102 92-93 1.4759960281155529 1102 94-95 1.395834819511915 1102 96-97 1.5744722804777886 1102 98-99 1.7064310629206787 1102 100-101 2.5044096991863007 1103 1 0.08462461004955912 1103 2 0.1533391459583342 1103 3 0.06326011875435711 1103 4 0.004145069155292447 1103 5 -0.15980705787611527 1103 6 -0.011479156728519513 1103 7 0.02506297877215502 1103 8 -0.0397691255744661 1103 9 0.00815928093681606 1103 10-11 0.042638126939905874 1103 12-13 0.05618536036834598 1103 14-15 0.09465273774916483 1103 16-17 0.0785518270647998 1103 18-19 0.01706604333486439 1103 20-21 0.05484916103224435 1103 22-23 0.07836409643777387 1103 24-25 0.038392580416605426 1103 26-27 0.03386601628546515 1103 28-29 0.07198033981039487 1103 30-31 0.001900789888111376 1103 32-33 -0.032268543194284405 1103 34-35 0.2253625274577047 1103 36-37 0.2575914929447052 1103 38-39 0.12641446402447087 1103 40-41 0.1673339892332848 1103 42-43 0.23275512526559083 1103 44-45 0.05283464992464104 1103 46-47 0.2987677469667318 1103 48-49 0.15194968240696483 1103 50-51 0.2202277882972794 1103 52-53 0.1626121936928584 1103 54-55 0.06947444646407774 1103 56-57 0.12107223833172753 1103 58-59 0.11414494051966528 1103 60-61 0.029776321453574894 1103 62-63 0.21910318047904553 1103 64-65 0.2060306940513712 1103 66-67 0.19844033276983453 1103 68-69 -0.05868632274379593 1103 70-71 0.06386581618701825 1103 72-73 0.1293574934045978 1103 74-75 0.21411260287177925 1103 76-77 0.009695280299688136 1103 78-79 -0.056564403771783844 1103 80-81 -0.09616996091039809 1103 82-83 -0.1815728723035086 1103 84-85 -0.07493459312842532 1103 86-87 0.1405811737365319 1103 88-89 0.35187454100986315 1103 90-91 0.3437707285938991 1103 92-93 0.4185996054942187 1103 94-95 0.5243165389970947 1103 96-97 0.7183244618732232 1103 98-99 0.968693059222467 1103 100-101 1.6795676054860742 1104 1 0.20621497381910103 1104 2 0.21383009591964708 1104 3 0.21736036145106397 1104 4 0.1334291712578235 1104 5 -0.03515187319011659 1104 6 0.05061813778864632 1104 7 0.146918770544346 1104 8 0.06978559369408543 1104 9 0.20956540644568378 1104 10-11 0.20896160468964808 1104 12-13 0.19157522496792012 1104 14-15 0.2098381584961757 1104 16-17 0.17152854700840692 1104 18-19 0.15953911593953052 1104 20-21 0.1870435571950253 1104 22-23 0.23322526335662985 1104 24-25 0.25190874374207084 1104 26-27 0.1997695391763301 1104 28-29 0.24160375809140788 1104 30-31 0.290180628151532 1104 32-33 0.2710229018040309 1104 34-35 0.3281362041636413 1104 36-37 0.2931510385944165 1104 38-39 0.2869798073014138 1104 40-41 0.24591261768689066 1104 42-43 0.28520953065864774 1104 44-45 0.280105769934373 1104 46-47 0.33997016337183084 1104 48-49 0.32006365112962243 1104 50-51 0.15450381964350157 1104 52-53 0.30986526643528833 1104 54-55 0.23029741103770363 1104 56-57 0.39020980397119587 1104 58-59 0.2842294136703387 1104 60-61 0.3409470288559362 1104 62-63 0.22592670070644516 1104 64-65 0.2205913487125457 1104 66-67 0.26359969289547536 1104 68-69 0.12317640322016388 1104 70-71 0.41134138004932197 1104 72-73 0.3886152134331624 1104 74-75 0.43231531547249347 1104 76-77 0.14505231081242798 1104 78-79 0.152737975207053 1104 80-81 0.34052063866758076 1104 82-83 0.4367102034467969 1104 84-85 0.4191137120127486 1104 86-87 0.6479428974572059 1104 88-89 0.6395151478205285 1104 90-91 0.4766966590269668 1104 92-93 0.8791525498852124 1104 94-95 1.0180335183529508 1104 96-97 1.337397851797519 1104 98-99 1.5122312879727318 1104 100-101 2.3612471034689584 1105 1 0.14011076172948123 1105 2 0.16427300256692945 1105 3 0.07407663787537189 1105 4 0.028442916636528537 1105 5 -0.06769313565838075 1105 6 0.02113667035097677 1105 7 0.05688390678685096 1105 8 0.04598660274181299 1105 9 0.023513016013730237 1105 10-11 0.07418842977048712 1105 12-13 -0.06933154871257585 1105 14-15 0.0936870751918022 1105 16-17 0.16964316556097714 1105 18-19 0.17463006716186413 1105 20-21 0.17894378997117144 1105 22-23 0.13803719919046387 1105 24-25 0.09367626251689387 1105 26-27 0.03608893753952458 1105 28-29 0.050856346541074515 1105 30-31 0.11910596072650037 1105 32-33 0.14642446698662326 1105 34-35 -0.001205176272627284 1105 36-37 0.10940797711413097 1105 38-39 0.13510917185413263 1105 40-41 0.08619237037540017 1105 42-43 0.04718538934983485 1105 44-45 0.21971826611621736 1105 46-47 0.39910952360608576 1105 48-49 0.331789354461165 1105 50-51 0.3542199591406572 1105 52-53 0.38276053823566514 1105 54-55 0.32818188665940085 1105 56-57 0.47028058477190626 1105 58-59 0.45368992072208414 1105 60-61 0.6104433476223292 1105 62-63 0.5744457809773493 1105 64-65 0.49149907035019424 1105 66-67 0.6249925579379791 1105 68-69 0.578364362057755 1105 70-71 0.6953756750325226 1105 72-73 0.7450550023547038 1105 74-75 0.8847410324967484 1105 76-77 0.7731186888788066 1105 78-79 0.8180143047169572 1105 80-81 0.8433697765532138 1105 82-83 0.7642109670789452 1105 84-85 0.7821813013577099 1105 86-87 0.919411493743322 1105 88-89 1.0039309428932555 1105 90-91 0.8661332564927626 1105 92-93 0.9726290247485494 1105 94-95 1.0842667579438547 1105 96-97 1.1276228336283403 1105 98-99 1.226843520833139 1105 100-101 1.650513595290196 1106 1 -0.13100679385409109 1106 2 -0.055200512052216766 1106 3 -0.01622723301102269 1106 4 -0.030742558082927474 1106 5 -0.07695356239453588 1106 6 -0.11576914228299273 1106 7 -0.17309269697615548 1106 8 -0.23100102667299893 1106 9 -0.04482220863217634 1106 10-11 -0.018026367298674018 1106 12-13 -0.18408238579350922 1106 14-15 -0.12382577872881484 1106 16-17 -0.002494297838623538 1106 18-19 0.14744700211472406 1106 20-21 -0.08933399481146864 1106 22-23 -0.1544255699713375 1106 24-25 -0.08837027778207585 1106 26-27 -0.2578798768176327 1106 28-29 -0.21459707813564677 1106 30-31 -0.20647929544128374 1106 32-33 -0.24421633773185647 1106 34-35 -0.8104606996058195 1106 36-37 -0.39012409814609583 1106 38-39 -0.5761672539854032 1106 40-41 -0.3943551295895702 1106 42-43 -0.5531319308703999 1106 44-45 -0.35971616137646123 1106 46-47 -0.3946897731058385 1106 48-49 -0.43487801269164095 1106 50-51 -0.46872306331692215 1106 52-53 -0.34448627104998053 1106 54-55 -0.6429587849667016 1106 56-57 -0.5660242107561899 1106 58-59 -0.45723275961806564 1106 60-61 -0.4930660879259392 1106 62-63 -0.37312143066753123 1106 64-65 -0.525801536270798 1106 66-67 -0.3203506102207214 1106 68-69 -0.6567580128704407 1106 70-71 -0.4335810209144668 1106 72-73 -0.38378961581030424 1106 74-75 -0.3455423841944345 1106 76-77 -0.45097593278863357 1106 78-79 -0.20079795098849829 1106 80-81 -0.16925928801759937 1106 82-83 -0.26320185742125446 1106 84-85 -0.2753461226745699 1106 86-87 -0.06604361210013465 1106 88-89 -0.10854471521347264 1106 90-91 -0.0995121013856064 1106 92-93 0.2845232793612489 1106 94-95 0.13068096940660823 1106 96-97 0.4471516866523402 1106 98-99 0.7843597370872182 1106 100-101 1.4424509998489583 1107 1 0.16217051462865584 1107 2 0.161656904147911 1107 3 0.13258929310335787 1107 4 0.054547286719007104 1107 5 0.010717403518576418 1107 6 0.1289193985716608 1107 7 0.05899574466511126 1107 8 0.06157391486197383 1107 9 0.1860916174609457 1107 10-11 0.13783519460338312 1107 12-13 0.2258734996859033 1107 14-15 0.2989224318870143 1107 16-17 0.2570461316135777 1107 18-19 0.25546645502380017 1107 20-21 0.24933619156794862 1107 22-23 0.23716521027103 1107 24-25 0.21226043584559307 1107 26-27 0.04586277076226253 1107 28-29 0.05410375809140788 1107 30-31 -0.11733430217361729 1107 32-33 -0.019745180478651037 1107 34-35 0.04284257443570283 1107 36-37 -0.06322752809436594 1107 38-39 -0.14790738579745977 1107 40-41 -0.24006946592292877 1107 42-43 -0.0581050015245026 1107 44-45 -0.062462245991305565 1107 46-47 0.1572395727945235 1107 48-49 -0.12324258642843233 1107 50-51 -0.06849717571150649 1107 52-53 -0.16039850264235156 1107 54-55 -0.15942554848452772 1107 56-57 -0.07706790007657816 1107 58-59 0.024607648574118457 1107 60-61 -0.04757321002926318 1107 62-63 -0.07073056538512645 1107 64-65 -0.03579551260131808 1107 66-67 -0.02658776563139753 1107 68-69 -0.051923132280833784 1107 70-71 0.12302021216611081 1107 72-73 0.19253027647231846 1107 74-75 0.34746130087395244 1107 76-77 0.22360241034792594 1107 78-79 0.23614043041607502 1107 80-81 0.3529211031665831 1107 82-83 0.407679015656484 1107 84-85 0.25666347976324744 1107 86-87 0.6691771376695463 1107 88-89 0.834729149147666 1107 90-91 0.7833904214689049 1107 92-93 0.8451860601705476 1107 94-95 1.0067528282401454 1107 96-97 1.0256028949295697 1107 98-99 1.335141042451827 1107 100-101 1.8709518148160065 1108 1 0.13237611410563943 1108 2 0.18235249156055033 1108 3 0.04451109722949553 1108 4 0.1263731489157962 1108 5 -0.11953126367379951 1108 6 -0.012583994948634825 1108 7 0.08444883185870111 1108 8 0.09528248998783795 1108 9 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scores pass #Quality Count 2 3.0 3 5.0 4 11.0 5 27.0 6 53.0 7 115.0 8 282.0 9 575.0 10 997.0 11 1575.0 12 2626.0 13 3666.0 14 4866.0 15 6779.0 16 9229.0 17 13462.0 18 12226.0 19 7342.0 20 6187.0 21 6082.0 22 6657.0 23 7291.0 24 8316.0 25 9737.0 26 11976.0 27 14765.0 28 18152.0 29 22994.0 30 29113.0 31 37063.0 32 47875.0 33 63692.0 34 89040.0 35 129604.0 36 203605.0 37 308692.0 38 231849.0 39 18551.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83844155999862 19.72897998822043 15.194819986901598 22.23775846487935 2 15.58766846356139 20.56579338631888 40.169922274378855 23.676615875740872 3 19.59244420375992 24.54420368501986 29.854967601216703 26.008384510003513 4 13.107069908117936 17.115291132148535 36.92996904307679 32.84766991665674 5 13.594783678318839 37.754656386645465 34.00160463020465 14.648955304831047 6 31.902708524801117 36.70151848464625 16.89058566483981 14.505187325712821 7 28.605530301157756 33.0988906291079 20.16843921829462 18.127139851439726 8 26.474723256491554 35.62076008871372 18.995102169830894 18.909414484963833 9 26.30716787970168 17.22959131318624 20.140500838525902 36.32273996858618 10-11 25.068816049363296 27.167003350358517 26.692500305224925 21.07168029505326 12-13 25.970589623224487 24.380598619216368 27.703198183723472 21.945613573835672 14-15 22.641397787849577 25.89565726723697 26.289940460541345 25.173004484372107 16-17 21.735160638193488 29.231971693103265 26.87428702827768 22.158580640425562 18-19 21.697335217863188 27.574806322612634 29.56828087584199 21.159577583682186 20-21 22.466751979938273 26.48048299745446 29.997576923321795 21.055188099285473 22-23 22.186864268401198 26.37812379220359 29.87035944021164 21.564652499183566 24-25 22.058265618685986 26.560370555822498 29.97474674653231 21.406617078959204 26-27 21.833058938263356 26.755354449020192 30.050230027459477 21.36135658525697 28-29 21.622074079622486 27.051234036178418 29.964832777798584 21.36185910640051 30-31 21.855601532945865 26.700454992653977 29.862520986625114 21.581422487775047 32-33 21.795643859810752 26.778674851937822 29.79549555420233 21.630185734049096 34-35 22.135211271825455 26.741074137074826 29.76246385050795 21.36125074059177 36-37 22.070908057263942 26.65332665209077 29.76358737784449 21.512177912800794 38-39 21.841820833342695 26.95094904354118 29.713403156812205 21.493826966303917 40-41 22.021959716301858 26.960349222256923 29.53783328502166 21.479857776419557 42-43 21.950548071729866 26.930611040238873 29.33841116503727 21.780429722993993 44-45 22.03780145174712 27.073638228691742 29.176506893589227 21.71205342597191 46-47 22.25505418772344 27.378189038717498 28.669496457518306 21.69726031604075 48-49 22.333738552192713 27.467621814706373 28.47692387203473 21.721715761066182 50-51 22.3659463358403 27.63596366063183 28.251207230123114 21.74688277340476 52-53 22.457260067838604 27.877832085780597 27.85494957045661 21.809958275924185 54-55 22.283816487538957 27.79527983695371 28.011034536481304 21.90986913902602 56-57 22.292617451675216 27.952910722270786 28.00339455059281 21.75107727546119 58-59 22.422534662690875 27.578214355533486 28.116795909761283 21.882455072014356 60-61 22.350329305862342 27.748204041552537 28.371424655133282 21.53004199745184 62-63 22.160977498743524 27.72442271292903 28.43464179326947 21.67995799505798 64-65 22.136634406451744 27.790149062116832 28.23716313841632 21.836053393015106 66-67 22.177830408791678 27.873140281376184 28.025827646412466 21.923201663419672 68-69 22.07008413721714 27.709479799352998 28.17338423661186 22.047051826818 70-71 22.315949369364105 27.708318821105237 28.01534139127139 21.96039041825927 72-73 22.400576145465497 27.71741308289132 27.860775332105693 22.021235439537495 74-75 22.114621552782772 28.169687146205792 27.682001197680588 22.033690103330848 76-77 22.165733764468225 27.912051778131815 27.809398830482944 22.11281562691702 78-79 22.302691746792892 27.822169591208322 27.79966159356623 22.07547706843255 80-81 22.21415779267325 28.111889840391708 27.65105637785273 22.02289598908231 82-83 22.213529417051912 28.13341712381917 27.656665238533236 21.99638822059568 84-85 22.15934604447464 28.064817308084823 27.54934084252164 22.226495804918898 86-87 22.043156932051314 28.006315721667825 27.6361134642767 22.314413882004164 88-89 22.191425089563854 28.010135714612073 27.43560128561488 22.362837910209194 90-91 22.199816475963967 27.979437817187584 27.48617015299912 22.334575553849323 92-93 22.264776038797297 27.776102840248782 27.631912566013867 22.327208554940054 94-95 22.043306735696184 27.922200975071927 27.636562875211318 22.397929414020574 96-97 21.962562571109917 27.998226324844712 27.60727626263875 22.431934841406626 98-99 22.053730194041098 28.06300455853516 27.22477638010663 22.658488867317114 100-101 21.83270570380031 28.054628115455866 27.515680750188963 22.59698543055486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 443.0 1 309.5 2 203.5 3 263.5 4 256.0 5 246.5 6 432.5 7 912.5 8 1474.0 9 1799.0 10 1797.0 11 1688.0 12 1762.5 13 1941.5 14 2129.0 15 2287.5 16 2368.5 17 2485.0 18 2621.5 19 2802.0 20 2989.0 21 3293.0 22 3882.5 23 4692.5 24 5596.5 25 6835.0 26 8578.5 27 10430.0 28 12057.0 29 13785.5 30 16035.5 31 18467.5 32 20790.0 33 23260.5 34 26245.0 35 28614.0 36 31994.0 37 35707.5 38 37436.5 39 39059.0 40 40590.0 41 42643.5 42 44493.0 43 46241.0 44 48377.5 45 49491.5 46 50265.5 47 51943.0 48 54260.5 49 53184.5 50 50235.5 51 47731.0 52 45008.5 53 43146.5 54 41744.5 55 38981.0 56 34856.5 57 31473.5 58 28387.5 59 24859.5 60 20898.5 61 17008.0 62 13426.5 63 9940.0 64 7303.0 65 5586.5 66 4118.0 67 3191.5 68 2350.5 69 1548.0 70 1127.5 71 780.0 72 595.0 73 481.0 74 354.5 75 268.0 76 178.0 77 100.5 78 66.5 79 45.5 80 22.5 81 9.5 82 5.5 83 5.0 84 2.5 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04246933332134904 2 0.0 3 0.0 4 0.0 5 0.014530953552630888 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031458765423221513 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1235273365436255E-4 22-23 3.7450911218120847E-4 24-25 4.1196002339932936E-4 26-27 0.001722741916033559 28-29 0.0023219564955234925 30-31 3.3705820096308764E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009587433271838938 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.745091121812085E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1235273365436255E-4 74-75 3.745091121812085E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.7450911218120847E-4 84-85 4.1196002339932936E-4 86-87 0.0 88-89 0.0 90-91 0.00793959317824162 92-93 0.002958621986231547 94-95 0.0 96-97 0.0 98-99 2.247054673087251E-4 100-101 0.012283898879543637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1335081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.37935574412124 #Duplication Level Percentage of deduplicated Percentage of total 1 77.82739260828404 46.991678249329745 2 12.895748054352191 15.572739187205805 3 4.16759781324845 7.549106128936497 4 1.7846093241541299 4.31014244989512 5 0.9268360759788127 2.7980882574005057 6 0.548052114553931 1.9854620154581817 7 0.3607708446977985 1.5248177821880846 8 0.2569038538553179 1.240935134717278 9 0.18849991917233355 1.0243353309699987 >10 0.9614359025140509 10.297686002744117 >50 0.056363571369088206 2.335390068919195 >100 0.0242948694613414 2.610331212460856 >500 8.721115424009932E-4 0.34009243856676885 >1k 3.737620896004257E-4 0.5277037063804211 >5k 2.491747264002838E-4 0.8914920348274226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6348 0.47547676882526224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5503 0.41218472886663804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3746 0.2805822268461614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1853 0.13879307697435586 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1416 0.10606098056971824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.745091121812085E-5 7.49018224362417E-5 14-15 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 16-17 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 18-19 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 20-21 0.0 0.0 0.0 7.49018224362417E-5 7.49018224362417E-5 22-23 0.0 0.0 0.0 1.498036448724834E-4 7.49018224362417E-5 24-25 0.0 0.0 0.0 3.3705820096308764E-4 7.49018224362417E-5 26-27 0.0 0.0 0.0 4.119600233993293E-4 7.49018224362417E-5 28-29 0.0 0.0 0.0 0.0013107818926342296 7.49018224362417E-5 30-31 0.0 0.0 0.0 0.0028837201637953057 7.49018224362417E-5 32-33 0.0 0.0 0.0 0.0057674403275906104 7.49018224362417E-5 34-35 0.0 0.0 0.0 0.009250375070875849 7.49018224362417E-5 36-37 0.0 0.0 0.0 0.01322017165999666 7.49018224362417E-5 38-39 0.0 0.0 0.0 0.016703106403281896 7.49018224362417E-5 40-41 0.0 0.0 0.0 0.019998786590476533 7.49018224362417E-5 42-43 3.745091121812085E-5 0.0 0.0 0.02340681951132553 7.49018224362417E-5 44-45 7.49018224362417E-5 0.0 0.0 0.0274889688341007 7.49018224362417E-5 46-47 7.49018224362417E-5 0.0 0.0 0.03250739093732889 7.49018224362417E-5 48-49 7.49018224362417E-5 0.0 0.0 0.03887404584440944 7.49018224362417E-5 50-51 7.49018224362417E-5 0.0 0.0 0.04344305701302019 7.49018224362417E-5 52-53 7.49018224362417E-5 0.0 0.0 0.0549779376682014 7.49018224362417E-5 54-55 7.49018224362417E-5 0.0 0.0 0.07928357904876185 7.49018224362417E-5 56-57 7.49018224362417E-5 0.0 0.0 0.12014252318773169 7.49018224362417E-5 58-59 7.49018224362417E-5 0.0 0.0 0.16354812928953374 7.49018224362417E-5 60-61 7.49018224362417E-5 0.0 0.0 0.19785316396533242 7.49018224362417E-5 62-63 7.49018224362417E-5 0.0 0.0 0.24039739910911773 7.49018224362417E-5 64-65 7.49018224362417E-5 0.0 0.0 0.29455141673052043 7.49018224362417E-5 66-67 7.49018224362417E-5 0.0 0.0 0.37754263598987625 7.49018224362417E-5 68-69 7.49018224362417E-5 0.0 0.0 0.4834163620035039 7.49018224362417E-5 70-71 7.49018224362417E-5 0.0 0.0 0.5762197200020074 7.49018224362417E-5 72-73 7.49018224362417E-5 0.0 0.0 0.6846026570672491 7.49018224362417E-5 74-75 7.49018224362417E-5 0.0 0.0 0.8041085147642728 7.49018224362417E-5 76-77 7.49018224362417E-5 0.0 0.0 0.938707089682199 7.49018224362417E-5 78-79 7.49018224362417E-5 0.0 0.0 1.0615835293888534 7.49018224362417E-5 80-81 7.49018224362417E-5 0.0 0.0 1.1965566134189611 7.49018224362417E-5 82-83 7.49018224362417E-5 0.0 0.0 1.3516408367731996 7.49018224362417E-5 84-85 7.49018224362417E-5 0.0 0.0 1.5671333799222669 7.49018224362417E-5 86-87 7.49018224362417E-5 0.0 0.0 1.8119125356439048 7.49018224362417E-5 88-89 7.49018224362417E-5 0.0 0.0 2.1093476725382203 7.49018224362417E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5790 0.0 31.10595 1 GTATCAA 8280 0.0 30.819586 1 TCAACGC 9945 0.0 25.651096 4 ATCAACG 9965 0.0 25.647285 3 TATCAAC 9995 0.0 25.617832 2 GTATAGG 300 0.0 25.344372 1 CAACGCA 10130 0.0 25.18264 5 AACGCAG 10195 0.0 25.018335 6 TATACCG 95 0.0012259542 25.002464 5 GTACATG 5485 0.0 23.478779 1 GAGTACT 6195 0.0 23.002104 12-13 TACATGG 5570 0.0 22.856833 2 ACGCAGA 11380 0.0 22.288795 7 CGCAGAG 11350 0.0 22.26401 8 ACATGGG 5565 0.0 21.767643 3 GTACTTT 6550 0.0 21.428291 14-15 GCAGAGT 12080 0.0 20.682661 9 AGAGTAC 9230 0.0 20.533304 10-11 AGTACTT 6355 0.0 20.068567 12-13 GGACCGA 380 0.0 19.998974 6 >>END_MODULE