FastQCFastQC Report
Thu 26 May 2016
SRR937791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829235
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.8908210579630623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56280.6786978359572376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.4934668700669894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.1558062551628911No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.1361495836524025No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9590.11564876060465368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9450.1139604575301332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.041.6227651
GTATCAA50600.036.5450821
TCAACGC62400.029.5527734
ATCAACG62500.029.4276643
TATCAAC63450.029.1351012
CAACGCA63800.028.9787735
AACGCAG64100.028.9756766
GTATAGC4100.027.8264141
GTACATG38100.027.6986031
TACATGG37700.027.479812
TACGCGC350.00840051127.13762992-93
ACATGGG37150.025.5856233
ACGCAGA72600.025.5177787
CGCAGAG72550.025.4044138
GCAGAGT76500.023.5959269
GAGTACT48500.023.45591512-13
CATGGGG11950.021.874894
GTACTTT52100.021.83648114-15
CATGGGA23100.021.8094624
GTGTAGC5900.021.7541221
Produced by FastQC (version 0.11.5)