##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937791_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 829235 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88138103191496 33.0 31.0 34.0 30.0 34.0 2 32.017348520021464 33.0 31.0 34.0 30.0 34.0 3 32.02069980162439 34.0 31.0 34.0 30.0 34.0 4 35.61198815775986 37.0 35.0 37.0 33.0 37.0 5 35.61951437167992 37.0 35.0 37.0 33.0 37.0 6 35.6848987319638 37.0 35.0 37.0 33.0 37.0 7 35.640216585165845 37.0 35.0 37.0 33.0 37.0 8 35.62096510639324 37.0 35.0 37.0 33.0 37.0 9 37.29119007277792 39.0 37.0 39.0 34.0 39.0 10-11 37.17129522994085 39.0 37.0 39.0 34.0 39.0 12-13 37.076111114460915 39.0 37.0 39.0 33.0 39.0 14-15 38.23326982097958 40.0 38.0 41.0 33.5 41.0 16-17 38.183677124096306 40.0 38.0 41.0 33.0 41.0 18-19 38.175626330292374 40.0 38.0 41.0 33.5 41.0 20-21 38.123890091469846 40.0 38.0 41.0 34.0 41.0 22-23 38.039551514347565 40.0 38.0 41.0 33.0 41.0 24-25 38.06359295012874 40.0 38.0 41.0 33.0 41.0 26-27 37.96367736528246 40.0 38.0 41.0 33.0 41.0 28-29 37.833079886883695 40.0 38.0 41.0 33.0 41.0 30-31 37.573670310587474 40.0 37.0 41.0 33.0 41.0 32-33 37.387001875222346 40.0 36.5 41.0 33.0 41.0 34-35 37.37816481455799 40.0 36.5 41.0 33.0 41.0 36-37 37.23886473677546 40.0 36.0 41.0 32.0 41.0 38-39 37.08054110113538 40.0 36.0 41.0 31.5 41.0 40-41 36.84173063124446 40.0 36.0 41.0 31.0 41.0 42-43 36.55719850223399 39.0 35.5 41.0 30.5 41.0 44-45 36.25608000144712 39.0 35.0 41.0 30.0 41.0 46-47 35.85395997515782 39.0 35.0 41.0 28.5 41.0 48-49 35.487286474883476 39.0 35.0 41.0 27.0 41.0 50-51 34.57137542433689 38.0 34.0 40.0 25.0 40.5 52-53 34.64625407755341 38.5 35.0 40.0 24.5 40.5 54-55 34.94844163596568 39.0 35.0 40.0 24.0 41.0 56-57 34.760085500491414 39.0 35.0 40.0 23.5 41.0 58-59 34.55654790258491 38.5 34.5 40.0 23.0 41.0 60-61 34.28324962163922 38.0 34.0 40.0 22.0 41.0 62-63 33.948911345999626 38.0 34.0 40.0 20.5 41.0 64-65 33.61307108358909 38.0 34.0 40.0 18.5 41.0 66-67 33.22682231213106 37.0 33.0 40.0 15.5 41.0 68-69 32.77591997443427 37.0 32.5 39.5 10.5 41.0 70-71 32.36796264026482 36.0 32.0 39.0 8.0 40.5 72-73 31.926882608669438 36.0 31.5 39.0 6.5 40.0 74-75 31.42458048683425 35.0 31.0 38.0 2.0 40.0 76-77 30.960148811856712 35.0 31.0 37.5 2.0 39.5 78-79 30.40163041839768 35.0 30.0 37.0 2.0 39.0 80-81 29.846056304907535 34.0 29.5 36.0 2.0 39.0 82-83 29.33288151127244 34.0 29.0 36.0 2.0 37.5 84-85 28.813948398222458 34.0 29.0 35.5 2.0 37.0 86-87 28.28703925907614 34.0 28.0 35.0 2.0 36.5 88-89 27.855757414966806 34.0 27.0 35.0 2.0 36.0 90-91 27.34513919455884 33.5 26.0 35.0 2.0 36.0 92-93 26.780769022050443 33.0 25.5 35.0 2.0 35.0 94-95 26.111347205556932 32.5 24.5 35.0 2.0 35.0 96-97 25.387552382617713 32.0 21.5 34.5 2.0 35.0 98-99 24.468558370063974 32.0 10.0 34.0 2.0 35.0 100-101 21.82793418029871 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30787999349493944 1101 2 0.4353272539681292 1101 3 0.37718702495748246 1101 4 0.25754252426824564 1101 5 0.19893554301815186 1101 6 0.4146552495410134 1101 7 0.2918258542513428 1101 8 0.4033812257937086 1101 9 0.3007757185441804 1101 10-11 0.27250573736843364 1101 12-13 0.26471510707330737 1101 14-15 0.5306106107982203 1101 16-17 0.5586484192756629 1101 18-19 0.5365588400315318 1101 20-21 0.6047283494127598 1101 22-23 0.4380690583263416 1101 24-25 0.41957641861818473 1101 26-27 0.4175521582199977 1101 28-29 0.392579953892195 1101 30-31 0.6760536477672687 1101 32-33 0.5754847160076721 1101 34-35 0.48080814133832206 1101 36-37 0.612839965987753 1101 38-39 0.6311759103041794 1101 40-41 0.7639561789090692 1101 42-43 0.8651873511923043 1101 44-45 0.8494302390569075 1101 46-47 0.7012425024528568 1101 48-49 0.9317225488264853 1101 50-51 0.941675281104402 1101 52-53 0.9527294372516195 1101 54-55 1.142349503988406 1101 56-57 0.9521771465421338 1101 58-59 0.8930144459535683 1101 60-61 1.0505368113139184 1101 62-63 1.265172841810191 1101 64-65 0.8866253570783371 1101 66-67 1.184173036056464 1101 68-69 0.9948969100627352 1101 70-71 1.2087198762752251 1101 72-73 1.010760975326665 1101 74-75 0.9927803214582269 1101 76-77 1.0103807359630181 1101 78-79 1.0002166339271845 1101 80-81 1.0427933288143443 1101 82-83 0.9656257711672929 1101 84-85 1.126759558646043 1101 86-87 1.042511240350862 1101 88-89 0.9798603017729874 1101 90-91 0.907927678318071 1101 92-93 0.9227548642500807 1101 94-95 1.0456468927699376 1101 96-97 1.5919313111359763 1101 98-99 1.5611898534136266 1101 100-101 1.636274729708738 1102 1 0.28961774078591773 1102 2 0.4146944872915235 1102 3 0.5201748965152895 1102 4 0.35836765599108134 1102 5 0.3728141591827736 1102 6 0.3513472666401327 1102 7 0.3492326541320452 1102 8 0.30633032610188593 1102 9 0.4764055028169736 1102 10-11 0.3101715093139603 1102 12-13 0.36390637857147823 1102 14-15 0.46634875574304857 1102 16-17 0.5801162590211675 1102 18-19 0.6164310937346755 1102 20-21 0.5812973423543966 1102 22-23 0.530038936047788 1102 24-25 0.5933335018272459 1102 26-27 0.634018009696284 1102 28-29 0.5951269267523145 1102 30-31 0.6436955604822501 1102 32-33 0.7312897165047403 1102 34-35 0.8289602936397458 1102 36-37 0.8992188306860314 1102 38-39 0.9568255772690861 1102 40-41 0.996376648140604 1102 42-43 0.8884391654879593 1102 44-45 1.0451156948676257 1102 46-47 1.151819349057888 1102 48-49 1.1951294481206531 1102 50-51 1.3211441646912405 1102 52-53 1.3532250133526276 1102 54-55 1.2376320867501107 1102 56-57 1.2550283245916347 1102 58-59 1.409123651640023 1102 60-61 1.4973682557914998 1102 62-63 1.4393529292940386 1102 64-65 1.5512920734517337 1102 66-67 1.5785961894514315 1102 68-69 1.54181285598181 1102 70-71 1.5844103027589682 1102 72-73 1.5195956010349896 1102 74-75 1.5239096583704494 1102 76-77 1.3093930632321324 1102 78-79 1.6764931027610643 1102 80-81 1.5451397350177452 1102 82-83 1.560332998525876 1102 84-85 1.6235370713212625 1102 86-87 1.6584444842120831 1102 88-89 1.6822772915333992 1102 90-91 1.5965649683071383 1102 92-93 1.5662927407785645 1102 94-95 1.8047074355674333 1102 96-97 1.8161287959754198 1102 98-99 1.5670502769149692 1102 100-101 1.8253864704384348 1103 1 0.19619275572913253 1103 2 0.3188875536394029 1103 3 0.32130759227142036 1103 4 0.22387871397075543 1103 5 0.21189664573388 1103 6 0.0861978344823271 1103 7 0.20752016160365372 1103 8 0.18316535299967285 1103 9 0.28117537729396247 1103 10-11 0.18580809030618894 1103 12-13 0.3302811544232398 1103 14-15 0.4285543743921849 1103 16-17 0.3257139876523709 1103 18-19 0.2175996532086799 1103 20-21 0.14537235729761733 1103 22-23 0.1272445546969223 1103 24-25 0.19225310732634426 1103 26-27 0.2251656486681881 1103 28-29 0.15027337026695875 1103 30-31 0.3345084714206834 1103 32-33 0.2528393279811283 1103 34-35 0.45138258891768857 1103 36-37 0.4939020345114997 1103 38-39 0.4822469759540837 1103 40-41 0.5975885429403647 1103 42-43 0.4743746107957918 1103 44-45 0.443442054700256 1103 46-47 0.8347138499544755 1103 48-49 0.8434438533806627 1103 50-51 0.5915013075483841 1103 52-53 0.6276452165803619 1103 54-55 0.6932313097028882 1103 56-57 0.6867916240536829 1103 58-59 0.6711394914487485 1103 60-61 0.8476417166402683 1103 62-63 0.9176598629461594 1103 64-65 0.7448201666974015 1103 66-67 0.8084422743289039 1103 68-69 0.8241544578945437 1103 70-71 0.9103577026394234 1103 72-73 0.9058343936231559 1103 74-75 0.7695103756448063 1103 76-77 0.9767242048938201 1103 78-79 1.0960657267897567 1103 80-81 1.1706224008874422 1103 82-83 1.1807633631403434 1103 84-85 1.2589255949315437 1103 86-87 1.2520144184619326 1103 88-89 1.1600791444921548 1103 90-91 1.1228844542605145 1103 92-93 0.8513002123864553 1103 94-95 0.847845510881239 1103 96-97 1.0290591605898847 1103 98-99 1.2279887108659544 1103 100-101 1.2195735594999988 1104 1 0.1748394156627704 1104 2 0.2869358666018691 1104 3 0.1785246502098765 1104 4 0.15738243431620447 1104 5 0.20549888824681517 1104 6 0.15949997599973642 1104 7 0.25103068368869685 1104 8 0.14374411920533703 1104 9 0.2142380151322456 1104 10-11 0.18918628763908174 1104 12-13 0.18851228990151725 1104 14-15 0.2854127951519203 1104 16-17 0.26551034769111226 1104 18-19 0.1873916848676842 1104 20-21 0.26589093841351286 1104 22-23 0.1900143055059189 1104 24-25 0.15006995502921683 1104 26-27 0.11820520181451144 1104 28-29 0.2377870252744856 1104 30-31 0.3221684668861897 1104 32-33 0.37255818941114427 1104 34-35 0.31091842171856143 1104 36-37 0.38224838733628275 1104 38-39 0.30475661175184854 1104 40-41 0.5333347762194194 1104 42-43 0.3853357172121008 1104 44-45 0.47959845348831465 1104 46-47 0.42968880718596125 1104 48-49 0.5272969358053814 1104 50-51 0.4550481055779443 1104 52-53 0.5544811709880975 1104 54-55 0.5371025300998653 1104 56-57 0.5675406398625782 1104 58-59 0.6745300555809024 1104 60-61 0.8120603740181025 1104 62-63 0.7752273135304932 1104 64-65 0.9463536498064116 1104 66-67 0.8961208199933068 1104 68-69 0.6956675357847715 1104 70-71 0.7243610416752233 1104 72-73 0.8292138276359715 1104 74-75 1.1046485746266086 1104 76-77 0.8650334573208021 1104 78-79 0.8134142850270756 1104 80-81 0.9321224936384347 1104 82-83 1.080431520693363 1104 84-85 0.9262710614690448 1104 86-87 0.8725700999756292 1104 88-89 0.9452699023708391 1104 90-91 1.138720140720931 1104 92-93 1.1524626915174778 1104 94-95 1.2129340365526566 1104 96-97 1.3454760866158146 1104 98-99 1.3530108680479742 1104 100-101 0.9826524802906498 1105 1 -0.03725052476847779 1105 2 0.18012201596722477 1105 3 0.06711789269186497 1105 4 -0.03461114136727872 1105 5 0.1212900020746801 1105 6 0.07326071815212032 1105 7 0.04201506636701424 1105 8 8.749906830445298E-4 1105 9 0.12934954174641433 1105 10-11 0.04500293093369834 1105 12-13 0.08643157843243898 1105 14-15 0.16446484889564061 1105 16-17 0.04498070114838271 1105 18-19 0.10841753794740328 1105 20-21 0.27701423495086175 1105 22-23 0.03347133090353793 1105 24-25 0.11698052998157493 1105 26-27 0.1561626047605884 1105 28-29 0.08982279049928366 1105 30-31 0.26116862269984153 1105 32-33 0.18307021702612047 1105 34-35 0.1496285244357125 1105 36-37 0.41553617514207275 1105 38-39 0.24962015495417944 1105 40-41 0.26080052718127433 1105 42-43 0.4397356548866398 1105 44-45 0.3861132617776022 1105 46-47 0.2858800025163504 1105 48-49 0.3206664658474523 1105 50-51 0.3095061737284297 1105 52-53 0.11695285485419049 1105 54-55 0.39201425567607373 1105 56-57 0.4407634653632755 1105 58-59 0.04315997419823958 1105 60-61 0.2517886589892697 1105 62-63 0.26956408674972465 1105 64-65 0.3246158482171211 1105 66-67 0.493087847428491 1105 68-69 0.4265773676258888 1105 70-71 0.4094155165233033 1105 72-73 0.7519200709689464 1105 74-75 0.760218313219255 1105 76-77 0.773141759791045 1105 78-79 0.5577079098517856 1105 80-81 0.5092316710622349 1105 82-83 0.618299630434116 1105 84-85 0.4495693966601557 1105 86-87 0.4976806677365886 1105 88-89 0.5959590781166462 1105 90-91 0.7641848848869088 1105 92-93 0.8795383090788746 1105 94-95 1.1543181411635395 1105 96-97 1.0967657441853653 1105 98-99 1.060411416991439 1105 100-101 0.9329377391210905 1106 1 -0.04978540925552721 1106 2 0.25894566164908284 1106 3 0.046034249927839 1106 4 0.12344088063877479 1106 5 0.09405068861079258 1106 6 0.10885348382719684 1106 7 0.08627189149043346 1106 8 0.2973872479034867 1106 9 0.04008055117287057 1106 10-11 0.06298167786880526 1106 12-13 0.06676213983148926 1106 14-15 0.015619272460376976 1106 16-17 -0.010169731583971497 1106 18-19 -0.1652309676082382 1106 20-21 -0.030779888924961085 1106 22-23 0.11566341794885915 1106 24-25 0.14936486408200977 1106 26-27 0.018342408701535362 1106 28-29 0.13098766176199206 1106 30-31 0.1391611587138044 1106 32-33 0.060263880638480316 1106 34-35 0.21965869098708168 1106 36-37 0.02132852949786468 1106 38-39 -0.1118701950437071 1106 40-41 -0.1934872645424548 1106 42-43 -0.07287335178200749 1106 44-45 -0.16706999178614979 1106 46-47 -0.1865206619948907 1106 48-49 -0.219352452956997 1106 50-51 -0.2707810909042294 1106 52-53 -0.3487603417178349 1106 54-55 -0.2497532406884133 1106 56-57 -0.08928778483085154 1106 58-59 0.07180878565494453 1106 60-61 -0.03614307330494171 1106 62-63 -0.014274453961000688 1106 64-65 0.07376167831495906 1106 66-67 0.06516637458545205 1106 68-69 -0.019675814921747303 1106 70-71 0.013846611131448583 1106 72-73 0.012668963842394021 1106 74-75 0.004337953479598866 1106 76-77 0.053973057705743344 1106 78-79 0.10195179300698953 1106 80-81 0.24513904421918298 1106 82-83 -0.302767305425494 1106 84-85 0.04230330508749702 1106 86-87 -0.22966618171773234 1106 88-89 -0.18705470625604548 1106 90-91 -0.09163688303301143 1106 92-93 0.1347515691842034 1106 94-95 0.6836822904997355 1106 96-97 0.8820855519853694 1106 98-99 1.3154752561910108 1106 100-101 1.1675320907755413 1107 1 0.2414321643045234 1107 2 0.22137110649225988 1107 3 0.19934577386944952 1107 4 0.18399925911464265 1107 5 0.2491876088841991 1107 6 0.3458098298004444 1107 7 0.25007555720967645 1107 8 0.23269918232605136 1107 9 0.38431547066262794 1107 10-11 0.28782883591019015 1107 12-13 0.320821858589845 1107 14-15 0.31911862253220846 1107 16-17 0.45157468805194156 1107 18-19 0.5787393160589858 1107 20-21 0.5420587112106219 1107 22-23 0.370702693512186 1107 24-25 0.3820484765631207 1107 26-27 0.3475963284836041 1107 28-29 0.41850313254010274 1107 30-31 0.4551195384335571 1107 32-33 0.6988012001978987 1107 34-35 0.5738213409525557 1107 36-37 0.6959321977048702 1107 38-39 0.6039868083209896 1107 40-41 0.4850066435471234 1107 42-43 0.48643181592434104 1107 44-45 0.7883632832920568 1107 46-47 0.8605664781345936 1107 48-49 0.9246517264853722 1107 50-51 0.8241379634969377 1107 52-53 0.7607789867104628 1107 54-55 0.893997497866323 1107 56-57 1.079608021422139 1107 58-59 0.8895428064401969 1107 60-61 0.9717446912531358 1107 62-63 1.1320573390322153 1107 64-65 1.245872918651557 1107 66-67 1.17517060378308 1107 68-69 1.1734010507452481 1107 70-71 0.6332343725798211 1107 72-73 0.8528052932720165 1107 74-75 0.8760502187746759 1107 76-77 0.8870021583171095 1107 78-79 1.3666653581584 1107 80-81 1.3126833730517369 1107 82-83 1.275891106013244 1107 84-85 1.2592720276832274 1107 86-87 1.3219642361643125 1107 88-89 1.2306982837244895 1107 90-91 1.5157163233829287 1107 92-93 1.7393324743568854 1107 94-95 1.9926174034130923 1107 96-97 1.8613251671283813 1107 98-99 1.9901784349305878 1107 100-101 1.925489823676834 1108 1 -0.15452745827731107 1108 2 -0.1350479014672601 1108 3 -0.12460262104911024 1108 4 -0.053751781948029986 1108 5 0.01008582194624097 1108 6 -0.16576046169710423 1108 7 0.0128977595184665 1108 8 -0.026327753523403885 1108 9 0.02943828970222029 1108 10-11 0.19977338285025326 1108 12-13 0.13985485632323247 1108 14-15 0.1997621046891851 1108 16-17 0.2993258608947045 1108 18-19 0.38620861129908235 1108 20-21 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-0.3759763948959467 2316 78-79 -0.24557586275617638 2316 80-81 -0.5102420383812678 2316 82-83 -0.36378030196673095 2316 84-85 -0.517201844934899 2316 86-87 -0.37570576209333595 2316 88-89 -0.4872424129000983 2316 90-91 -0.4134966080475415 2316 92-93 -0.9061579981376937 2316 94-95 -0.842484682659169 2316 96-97 -0.6411002680639868 2316 98-99 -0.9109053881096614 2316 100-101 -0.7696874211871823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2826.0 3 436.0 4 450.0 5 414.0 6 525.0 7 637.0 8 768.0 9 1008.0 10 1331.0 11 1947.0 12 2873.0 13 4027.0 14 4700.0 15 6522.0 16 8634.0 17 11275.0 18 9819.0 19 6357.0 20 5716.0 21 5595.0 22 6166.0 23 6756.0 24 7453.0 25 8154.0 26 9285.0 27 10875.0 28 12689.0 29 15095.0 30 18421.0 31 22727.0 32 29212.0 33 37966.0 34 51802.0 35 73422.0 36 106711.0 37 160128.0 38 155276.0 39 21234.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.681894888520326 19.92357682692726 15.484698441557185 21.909829842995226 2 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19.39362460289659 20-21 20.377504573260033 26.755065915941394 33.70774981112089 19.15967969967768 22-23 19.60533030561989 27.17181569197042 33.566612212113064 19.656241790296622 24-25 19.340462758347037 27.01225474357201 34.10757673030082 19.539705767780127 26-27 19.365514575771666 27.282390100433012 33.84495992946136 19.50713539433396 28-29 19.158897413241743 27.600120547488523 33.790439424585045 19.45054261468469 30-31 19.384352617811956 27.37739264250163 33.93265783000242 19.30559690968399 32-33 19.10710694266358 27.383752919426545 33.80434723524946 19.704792902660415 34-35 19.48199334055287 27.525271895212455 33.631415969023244 19.36131879521143 36-37 19.367314192402528 27.443017151470535 33.48833529702704 19.70133335909989 38-39 19.226874131528746 27.779606226602983 33.52593441619105 19.46758522567722 40-41 19.525708122314835 27.58038430831629 33.477689512023375 19.416218057345496 42-43 19.29521638797946 27.612771840060297 33.209405247859195 19.882606524101053 44-45 19.5744092322057 27.86077043355685 32.75770955972723 19.80711077451021 46-47 19.61395954184873 27.909058241257167 32.267051579764285 20.20993063712982 48-49 19.625542852345973 27.960904364378862 32.2270851539131 20.186467629362067 50-51 19.78323613021051 28.1770685180857 31.862754867554326 20.17694048414946 52-53 19.770018718142467 28.31706443474411 31.698953801401657 20.21396304571177 54-55 19.785315067500445 28.20682221007721 31.580183765471055 20.427678956951294 56-57 19.97030065902584 28.2824927880362 31.286123982368807 20.46108257056915 58-59 20.041777355320693 28.103635378740893 31.427405051327934 20.42718221461048 60-61 20.14866022822588 28.04604614397059 31.55451931803977 20.25077430976376 62-63 19.992970577276242 27.92752206206062 31.716223895568383 20.36328346509475 64-65 20.003478414205457 27.928525834774113 31.691916370709578 20.376079380310845 66-67 19.91208746083852 27.932370510470033 31.608054281373267 20.547487747318176 68-69 20.041501995381154 27.817147233039258 31.603781561325356 20.53756921025423 70-71 19.96915242855176 27.62723717361844 31.728671815175456 20.674938582654338 72-73 19.859996787365894 27.54477457816061 31.753868748195913 20.841359886277584 74-75 19.812321737959405 27.679373206056578 31.66338450129505 20.84492055468897 76-77 19.63707709922558 27.67955921214727 31.557709681003704 21.125654007623446 78-79 19.627360251035412 27.569386820882418 31.513858038894544 21.289394889187626 80-81 19.603022622565316 27.565155898890826 31.511438100914724 21.32038337762914 82-83 19.724300273199624 27.36978330028045 31.55836109212214 21.34755533439779 84-85 19.915362216621034 27.292303493120162 31.34176490551317 21.450569384745634 86-87 19.660531418264686 27.206397839690492 31.415909698869427 21.717161043175395 88-89 19.81877150850437 26.983311302747353 31.378941577784474 21.818975610963804 90-91 19.76349270380668 26.90098385682504 31.73566305862801 21.59986038074027 92-93 19.670256211756794 27.036744276765944 31.697335709321123 21.595663802156142 94-95 19.5972043073088 27.218993511983264 31.43046759131775 21.75333458939018 96-97 19.569959093758264 27.215754760619376 31.53717854421123 21.677107601411127 98-99 19.878057175568923 27.089374416523455 31.29940302725447 21.733165380653155 100-101 20.1991689319169 26.577091165101418 31.60745618537093 21.616283717610745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1262.0 1 813.5 2 471.0 3 528.0 4 387.0 5 379.0 6 740.0 7 1628.0 8 2617.0 9 2905.5 10 2232.0 11 1562.0 12 1475.0 13 1506.5 14 1548.0 15 1565.0 16 1624.5 17 1711.5 18 1825.0 19 1995.5 20 2226.0 21 2602.5 22 3060.5 23 3725.0 24 4596.0 25 5625.0 26 7037.5 27 8828.5 28 10715.5 29 12612.5 30 14751.5 31 17292.0 32 19915.5 33 22474.0 34 25264.5 35 28090.5 36 30532.0 37 32016.0 38 32651.0 39 32778.5 40 32699.0 41 32466.5 42 31970.0 43 31608.5 44 31292.0 45 30872.5 46 30397.5 47 30007.5 48 30394.0 49 28873.0 50 25957.5 51 23593.0 52 20526.0 53 17946.5 54 15976.0 55 13781.5 56 11623.5 57 9631.0 58 7843.5 59 6438.0 60 5180.0 61 4099.5 62 3156.5 63 2214.5 64 1475.0 65 1073.0 66 763.5 67 560.5 68 441.0 69 315.5 70 234.5 71 197.0 72 146.5 73 112.5 74 102.5 75 88.5 76 62.0 77 41.5 78 32.5 79 23.5 80 15.5 81 15.5 82 17.5 83 12.0 84 7.5 85 3.5 86 1.0 87 1.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24106556042617594 2 0.214293897387351 3 0.21357033892684224 4 0.21670575892238025 5 0.2243031227577225 6 0.16364480515173624 7 0.16460954976574793 8 0.16810674899154038 9 0.16497132899600234 10-11 0.1726289893697203 12-13 0.16653903899377137 14-15 0.1666596320705228 16-17 0.16991564514281235 18-19 0.16460954976574793 20-21 0.15972553015731367 22-23 0.16008730938756804 24-25 0.16298154322960318 26-27 0.15918286131193207 28-29 0.1622579847690944 30-31 0.16382569476686343 32-33 0.1658757770716383 34-35 0.16744348706940734 36-37 0.15538417939426097 38-39 0.1533340970894861 40-41 0.1574945582374116 42-43 0.16105205400157976 44-45 0.16286095015285174 46-47 0.16189620553884002 48-49 0.15876078554330195 50-51 0.16032849554107098 52-53 0.1722672101394659 54-55 0.15405765554999487 56-57 0.15321350401273465 58-59 0.15369587631974047 60-61 0.14947511863343924 62-63 0.15514299324075803 64-65 0.1533340970894861 66-67 0.15110312516958402 68-69 0.15954464054218645 70-71 0.15592684823964256 72-73 0.1512237182463355 74-75 0.15381646939649193 76-77 0.15230905593709865 78-79 0.15725337208390866 80-81 0.15417824862674634 82-83 0.15303261439760743 84-85 0.14905304286480914 86-87 0.1453749540238895 88-89 0.14706325709840998 90-91 0.15303261439760743 92-93 0.14814859478917314 94-95 0.15037956670907524 96-97 0.15001778747882083 98-99 0.14760592594379157 100-101 0.13795847980367446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 829235.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.71545875189251 #Duplication Level Percentage of deduplicated Percentage of total 1 85.61045200263234 64.82034647336182 2 10.124187969340937 15.331150731780802 3 2.354752887357364 5.348735853408188 4 0.8200844828168052 2.483722913271316 5 0.3614223260705631 1.3682628610804384 6 0.19977724042199793 0.9075735244043217 7 0.11626198020865841 0.6161980416831414 8 0.08162181493920759 0.494402652982734 9 0.05513427069529302 0.375706493878062 >10 0.25069699367766274 3.3225271606901536 >50 0.015045819301631586 0.7480962694793882 >100 0.008481776779542354 1.1691704456425396 >500 0.0012802681587024472 0.6478965979645708 >1k 4.801005595134178E-4 0.7891809505410976 >5k 3.200670396756118E-4 1.5770290298314733 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7387 0.8908210579630623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5628 0.6786978359572376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.4934668700669894 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.1558062551628911 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1129 0.1361495836524025 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 959 0.11564876060465368 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 945 0.1139604575301332 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.205930767514637E-4 0.0 3 0.0 0.0 0.0 1.205930767514637E-4 0.0 4 0.0 0.0 0.0 1.205930767514637E-4 0.0 5 0.0 0.0 0.0 1.205930767514637E-4 0.0 6 0.0 0.0 0.0 1.205930767514637E-4 0.0 7 0.0 0.0 0.0 1.205930767514637E-4 0.0 8 0.0 0.0 0.0 1.205930767514637E-4 0.0 9 0.0 0.0 0.0 1.205930767514637E-4 0.0 10-11 0.0 0.0 0.0 1.205930767514637E-4 0.0 12-13 0.0 0.0 0.0 1.205930767514637E-4 0.0 14-15 0.0 0.0 0.0 1.8088961512719554E-4 0.0 16-17 0.0 0.0 0.0 2.411861535029274E-4 0.0 18-19 0.0 0.0 0.0 2.411861535029274E-4 0.0 20-21 0.0 0.0 0.0 2.411861535029274E-4 0.0 22-23 0.0 0.0 0.0 4.823723070058548E-4 0.0 24-25 0.0 0.0 0.0 4.823723070058548E-4 0.0 26-27 0.0 0.0 0.0 0.0012662273058903688 0.0 28-29 0.0 0.0 0.0 0.004160461147925497 0.0 30-31 0.0 0.0 0.0 0.008260625757475264 0.0 32-33 0.0 0.0 0.0 0.013506424596163934 0.0 34-35 0.0 0.0 0.0 0.019114002665106995 0.0 36-37 0.0 0.0 0.0 0.02448039458054713 0.0 38-39 0.0 0.0 0.0 0.03491169571954874 0.0 40-41 0.0 0.0 0.0 0.0554728153056733 0.0 42-43 0.0 0.0 0.0 0.09321844832888143 0.0 44-45 0.0 0.0 0.0 0.12993904019970215 0.0 46-47 0.0 0.0 0.0 0.2082642435497778 0.0 48-49 0.0 0.0 0.0 0.29038812881752457 0.0 50-51 0.0 0.0 0.0 0.345378571816192 0.0 52-53 0.0 0.0 0.0 0.4179756040205732 0.0 54-55 0.0 0.0 0.0 0.5019083854395919 0.0 56-57 0.0 0.0 0.0 0.6632619221330504 0.0 58-59 0.0 0.0 0.0 0.8656171049220064 0.0 60-61 0.0 0.0 0.0 1.0638721231014125 0.0 62-63 0.0 0.0 0.0 1.280819068177296 0.0 64-65 0.0 0.0 0.0 1.5381043974265438 0.0 66-67 0.0 0.0 0.0 1.8642483735008772 0.0 68-69 0.0 0.0 0.0 2.212279993005602 0.0 70-71 0.0 0.0 0.0 2.4673343503349474 0.0 72-73 0.0 0.0 0.0 2.751451639161396 0.0 74-75 0.0 0.0 0.0 3.0879666198363553 0.0 76-77 0.0 0.0 0.0 3.4873105934988273 0.0 78-79 0.0 0.0 0.0 3.8937695586896357 0.0 80-81 0.0 0.0 0.0 4.394833792591967 0.0 82-83 0.0 0.0 0.0 4.953903296411752 0.0 84-85 0.0 0.0 0.0 5.60944726163271 0.0 86-87 0.0 0.0 0.0 6.273673928379772 0.0 88-89 0.0 0.0 0.0 7.130487738698921 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 41.622765 1 GTATCAA 5060 0.0 36.545082 1 TCAACGC 6240 0.0 29.552773 4 ATCAACG 6250 0.0 29.427664 3 TATCAAC 6345 0.0 29.135101 2 CAACGCA 6380 0.0 28.978773 5 AACGCAG 6410 0.0 28.975676 6 GTATAGC 410 0.0 27.826414 1 GTACATG 3810 0.0 27.698603 1 TACATGG 3770 0.0 27.47981 2 TACGCGC 35 0.008400511 27.137629 92-93 ACATGGG 3715 0.0 25.585623 3 ACGCAGA 7260 0.0 25.517778 7 CGCAGAG 7255 0.0 25.404413 8 GCAGAGT 7650 0.0 23.595926 9 GAGTACT 4850 0.0 23.455915 12-13 CATGGGG 1195 0.0 21.87489 4 GTACTTT 5210 0.0 21.836481 14-15 CATGGGA 2310 0.0 21.809462 4 GTGTAGC 590 0.0 21.754122 1 >>END_MODULE