FastQCFastQC Report
Thu 26 May 2016
SRR937791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829235
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55310.6670003075123456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.5519545122914493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.3862596248349382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14200.17124216898707847No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10710.12915518520081762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.1183018082931859No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.10262470831549561No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56850.032.3549921
GGTATCA36850.031.7291491
ATGGACG804.4843293E-429.696815
TATCAAC67150.027.1715892
TGCGTAA350.00839459727.14154860-61
ATCAACG67050.026.999523
TCAACGC67600.026.8501364
CAACGCA68050.026.6027575
AACGCAG69300.026.1134596
GAGTACT41250.023.8366512-13
GTACTAG1801.938115E-723.7646161
GTGTCGG500.001654418123.74885464-65
TACATGG40450.023.7280852
GTACATG41700.023.365691
ACGCAGA78000.023.0804067
CGCAGAG77800.022.8955218
GTACTTT43400.022.49027314-15
ACATGGG41300.021.7441083
GCAGAGT82400.021.5597279
AGTACTT42900.021.14827512-13
Produced by FastQC (version 0.11.5)