##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937791_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 829235 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12970267776927 33.0 31.0 34.0 31.0 34.0 2 32.41408285950303 34.0 31.0 34.0 31.0 34.0 3 32.50238593402353 34.0 31.0 34.0 31.0 34.0 4 36.13497621301561 37.0 35.0 37.0 35.0 37.0 5 36.053460116854694 37.0 35.0 37.0 35.0 37.0 6 36.090377275440616 37.0 35.0 37.0 35.0 37.0 7 36.0352216199268 37.0 35.0 37.0 35.0 37.0 8 36.02018486918666 37.0 35.0 37.0 35.0 37.0 9 37.70747857965474 39.0 37.0 39.0 35.0 39.0 10-11 37.62977623954609 39.0 37.0 39.0 35.0 39.0 12-13 37.535436275603416 39.0 37.0 39.0 35.0 39.0 14-15 38.75852140828595 40.0 38.0 41.0 35.0 41.0 16-17 38.726944714103965 40.0 38.0 41.0 34.5 41.0 18-19 38.72974669424228 40.0 38.0 41.0 34.5 41.0 20-21 38.65082214330075 40.0 38.0 41.0 34.0 41.0 22-23 38.585978040000725 40.0 38.0 41.0 34.0 41.0 24-25 38.60266209216929 40.0 38.0 41.0 34.0 41.0 26-27 38.46721375725819 40.0 38.0 41.0 34.0 41.0 28-29 38.314587541529235 40.0 38.0 41.0 34.0 41.0 30-31 38.326669158923586 40.0 38.0 41.0 34.0 41.0 32-33 38.23273981440725 40.0 38.0 41.0 34.0 41.0 34-35 38.08564279124735 40.0 37.0 41.0 34.0 41.0 36-37 37.93945564285154 40.0 37.0 41.0 33.0 41.0 38-39 37.80831790746893 40.0 37.0 41.0 33.0 41.0 40-41 37.61564031908928 40.0 37.0 41.0 33.0 41.0 42-43 37.385248451886376 40.0 37.0 41.0 33.0 41.0 44-45 37.06235807702279 40.0 36.5 41.0 31.5 41.0 46-47 36.68730456384499 40.0 36.0 41.0 31.0 41.0 48-49 36.365201661772595 40.0 36.0 41.0 30.0 41.0 50-51 36.09969791434274 39.0 36.0 41.0 30.0 41.0 52-53 35.8751614439815 39.0 35.0 41.0 29.0 41.0 54-55 35.664699391607925 39.0 35.0 40.5 28.0 41.0 56-57 35.40600915301452 39.0 35.0 40.0 27.5 41.0 58-59 35.1804144784048 38.5 35.0 40.0 27.0 41.0 60-61 34.88021248500124 38.0 34.5 40.0 26.0 41.0 62-63 34.54924719771838 38.0 34.0 40.0 26.0 41.0 64-65 34.17058915747647 38.0 34.0 40.0 25.0 41.0 66-67 33.78867510416227 37.0 33.5 40.0 23.5 41.0 68-69 33.357474057414365 36.5 33.0 39.0 22.0 40.5 70-71 32.97050052156506 36.0 32.5 39.0 22.0 40.0 72-73 32.47799839611208 35.5 32.0 38.5 20.5 40.0 74-75 32.0036823095986 35.0 31.5 38.0 18.5 39.5 76-77 30.702394978504284 34.0 30.0 36.0 18.0 39.0 78-79 31.273355562657148 35.0 31.0 37.0 18.0 39.0 80-81 31.055004914167878 35.0 31.0 36.0 15.0 39.0 82-83 30.659955259968527 34.0 31.0 36.0 9.5 37.5 84-85 30.238741731837173 34.0 31.0 35.5 7.0 37.0 86-87 29.852897550151646 34.0 30.0 35.0 2.0 36.5 88-89 29.409886823397468 34.0 30.0 35.0 2.0 36.0 90-91 28.876932353313595 34.0 29.0 35.0 2.0 36.0 92-93 28.44704215330998 34.0 29.0 35.0 2.0 35.0 94-95 27.989500563772634 34.0 29.0 35.0 2.0 35.0 96-97 27.460118663587522 33.0 27.0 35.0 2.0 35.0 98-99 26.63729642381231 33.0 25.5 35.0 2.0 35.0 100-101 24.040543995369227 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31718860278331107 1101 2 0.2126396958941541 1101 3 0.17554565858305438 1101 4 0.09988734765631335 1101 5 0.0996892412072441 1101 6 0.10677221821249105 1101 7 0.20384795271638012 1101 8 0.1461965506214966 1101 9 0.18614857620264047 1101 10-11 0.14557076856396378 1101 12-13 0.20597814344053944 1101 14-15 0.23808403164764513 1101 16-17 0.30876810351632855 1101 18-19 0.2544037414339684 1101 20-21 0.2356366083524577 1101 22-23 0.2252011687549924 1101 24-25 0.15081059167091126 1101 26-27 0.1573072797297428 1101 28-29 -0.02228835353666625 1101 30-31 0.08483525740567188 1101 32-33 0.2634236830302328 1101 34-35 0.3020876380357578 1101 36-37 0.2919294579531595 1101 38-39 0.28310087151346863 1101 40-41 0.32252308474850366 1101 42-43 0.2839521541856769 1101 44-45 0.30543924559676583 1101 46-47 0.23138337413800514 1101 48-49 0.3440661662997826 1101 50-51 0.49659642716072483 1101 52-53 0.3920499186400477 1101 54-55 0.53952436593557 1101 56-57 0.3849909167296772 1101 58-59 0.4115149717291402 1101 60-61 0.6477020212459408 1101 62-63 0.325978531661562 1101 64-65 0.5240508198445681 1101 66-67 0.45831661299767745 1101 68-69 0.5495689751908444 1101 70-71 0.4834871583102185 1101 72-73 0.43995977765810323 1101 74-75 0.2765821144281695 1101 76-77 0.15133358455645407 1101 78-79 0.3995579032650518 1101 80-81 0.5163738349926454 1101 82-83 0.5553454537170239 1101 84-85 0.4274843709870666 1101 86-87 0.332262930087758 1101 88-89 0.42907951656320265 1101 90-91 0.4306350733729545 1101 92-93 0.03189751729921397 1101 94-95 -0.1286069936939711 1101 96-97 -0.17295000961908258 1101 98-99 -0.2689878511897561 1101 100-101 -0.21493471959651345 1102 1 0.3850566811211209 1102 2 0.2522639130131523 1102 3 0.2199551426271924 1102 4 0.15840906949745204 1102 5 0.21228542373035708 1102 6 0.18928240809220398 1102 7 0.1644389657395422 1102 8 0.16122985413079505 1102 9 0.2613145966818138 1102 10-11 0.24517460592354468 1102 12-13 0.34144041603224906 1102 14-15 0.34097969722076016 1102 16-17 0.4526844967959818 1102 18-19 0.30937789393421866 1102 20-21 0.18362298870835758 1102 22-23 0.30561572288562644 1102 24-25 0.34094479984269555 1102 26-27 0.33995118071393193 1102 28-29 0.35665015923795806 1102 30-31 0.35610799823477635 1102 32-33 0.4628460908242502 1102 34-35 0.46131121890860527 1102 36-37 0.4041148143504145 1102 38-39 0.5295187559950278 1102 40-41 0.5349684073451755 1102 42-43 0.6436484460635938 1102 44-45 0.6469774203656229 1102 46-47 0.6029918839302297 1102 48-49 0.5941839712505725 1102 50-51 0.5311965365155302 1102 52-53 0.5737739965802007 1102 54-55 0.7071652845159448 1102 56-57 0.8573281271879765 1102 58-59 0.6525009047244694 1102 60-61 0.6023346885090817 1102 62-63 0.6850798339778947 1102 64-65 0.7820980909450697 1102 66-67 0.6400869181969995 1102 68-69 0.7471067523058679 1102 70-71 0.5770993464004803 1102 72-73 0.5692740733137356 1102 74-75 0.6093057947144835 1102 76-77 0.41873516523047627 1102 78-79 0.5844584898044651 1102 80-81 0.5244598773427782 1102 82-83 0.606433782576751 1102 84-85 0.6298817265890193 1102 86-87 0.5404864115471497 1102 88-89 0.4081731143363392 1102 90-91 0.666767392688989 1102 92-93 0.5184692441110244 1102 94-95 0.3598565712727222 1102 96-97 0.6825845862652002 1102 98-99 1.0442167417121269 1102 100-101 1.0304465310248219 1103 1 0.2935474163273355 1103 2 0.23310073309684043 1103 3 0.22120738410956164 1103 4 0.07885736597085469 1103 5 0.10246175367655752 1103 6 0.08133859458353498 1103 7 0.1211664014837126 1103 8 0.14034521575661074 1103 9 0.15709164390237618 1103 10-11 0.22777771411242753 1103 12-13 0.25829337478896974 1103 14-15 0.21088854360450426 1103 16-17 0.30110948185276243 1103 18-19 0.13424639363534396 1103 20-21 0.10336895404009994 1103 22-23 0.12807238755986106 1103 24-25 0.09771706523420676 1103 26-27 0.1827455620648024 1103 28-29 0.13647831225648588 1103 30-31 0.2964083568719502 1103 32-33 0.3674904422886911 1103 34-35 0.4550888638577959 1103 36-37 0.34012348141556714 1103 38-39 0.3016212664552711 1103 40-41 0.29637755259324194 1103 42-43 0.5645719367987994 1103 44-45 0.5881743719496129 1103 46-47 0.7201646275046514 1103 48-49 0.5737147542750805 1103 50-51 0.5895064588108099 1103 52-53 0.6406673020195299 1103 54-55 0.38373432217284886 1103 56-57 0.5181888564169057 1103 58-59 0.6262053279163453 1103 60-61 0.6873615862974916 1103 62-63 0.6394133665541091 1103 64-65 0.7166871525111205 1103 66-67 0.559487695244222 1103 68-69 0.47957686587430715 1103 70-71 0.7081124964303669 1103 72-73 0.5569264941146912 1103 74-75 0.6519701700880596 1103 76-77 0.6268657689363941 1103 78-79 0.522769906421324 1103 80-81 0.4402922144617243 1103 82-83 0.5188471119252256 1103 84-85 0.5068064127814864 1103 86-87 0.3327518030594021 1103 88-89 0.45774424404345027 1103 90-91 0.33127127792509015 1103 92-93 0.38022955492991173 1103 94-95 0.2779169418644756 1103 96-97 0.09841991202730682 1103 98-99 0.29725021451547207 1103 100-101 0.29755352444980687 1104 1 0.2508448584610221 1104 2 0.18497327261906804 1104 3 0.1521965219375403 1104 4 0.08949281333981673 1104 5 0.029600694666314098 1104 6 0.05189324553062846 1104 7 0.10067049283313878 1104 8 0.10142689846576758 1104 9 0.008038734612846099 1104 10-11 0.1867755911452207 1104 12-13 0.0889527313031877 1104 14-15 0.04064718490548813 1104 16-17 0.12147760024426191 1104 18-19 0.12294932250564017 1104 20-21 0.02689885077732157 1104 22-23 0.07689651106296225 1104 24-25 0.047250218561906365 1104 26-27 0.1544585698765033 1104 28-29 0.23387922327736987 1104 30-31 0.3326966681855197 1104 32-33 0.36319091841046003 1104 34-35 0.3193900366426021 1104 36-37 0.3438561936607556 1104 38-39 0.39012220427088806 1104 40-41 0.28773934330577333 1104 42-43 0.3882543573936488 1104 44-45 0.28415722332129434 1104 46-47 0.31742538146717436 1104 48-49 0.10800170524071717 1104 50-51 0.4044847138554317 1104 52-53 0.326668085250148 1104 54-55 0.3022391761415619 1104 56-57 0.27565325034068167 1104 58-59 0.3332028751678209 1104 60-61 0.3587386293957806 1104 62-63 0.2914837748645951 1104 64-65 0.42762764759531535 1104 66-67 0.5367987408571011 1104 68-69 0.490197885310792 1104 70-71 0.3009170803906329 1104 72-73 0.34004993538270156 1104 74-75 0.40414569619231244 1104 76-77 0.32872284995954004 1104 78-79 0.3393599676369803 1104 80-81 0.4360978083772622 1104 82-83 0.5549190042516265 1104 84-85 0.20005414038212166 1104 86-87 0.15802335735995499 1104 88-89 0.03456473995209919 1104 90-91 0.12817133357568622 1104 92-93 0.1942081603671788 1104 94-95 0.22224573383429913 1104 96-97 0.16887768971347583 1104 98-99 0.39433989442148487 1104 100-101 0.3027125408770388 1105 1 0.1386003029494134 1105 2 0.1505120881956401 1105 3 0.13074937272799758 1105 4 0.12521129403690168 1105 5 0.09121660204109361 1105 6 0.08642874107795251 1105 7 0.10665133974023888 1105 8 0.0462976330671836 1105 9 0.20942547607006645 1105 10-11 0.163059555523823 1105 12-13 0.21766738857308354 1105 14-15 0.1052888734459998 1105 16-17 0.2437985044428359 1105 18-19 0.27307097700686 1105 20-21 0.14595006552448098 1105 22-23 0.03913327589222604 1105 24-25 0.023190194350867444 1105 26-27 0.04792997807229682 1105 28-29 -0.039761901936877564 1105 30-31 -0.16255640913401237 1105 32-33 -0.058954395793755054 1105 34-35 0.0755308921793727 1105 36-37 0.02440765411405721 1105 38-39 -0.05030484497821419 1105 40-41 0.10597553282310912 1105 42-43 0.04100506754430455 1105 44-45 -0.08854482502888317 1105 46-47 -0.18707763292760404 1105 48-49 -0.19876180560031997 1105 50-51 -0.15515295948724628 1105 52-53 -0.15533184283585655 1105 54-55 -0.1862653724183687 1105 56-57 -0.15917169901987904 1105 58-59 -0.09940472614101026 1105 60-61 -0.3124897814248726 1105 62-63 -0.5433178024489322 1105 64-65 -0.29249089063296196 1105 66-67 -0.25182566435426423 1105 68-69 -0.12084510727008535 1105 70-71 -0.26759477942503196 1105 72-73 -0.07195550610927626 1105 74-75 -0.19564815036720162 1105 76-77 -0.4512257315369901 1105 78-79 -0.18361415531197522 1105 80-81 -0.29167237847925875 1105 82-83 0.09376694211794145 1105 84-85 -0.0867484437272843 1105 86-87 -0.2039730948831604 1105 88-89 -0.10976484186804214 1105 90-91 -0.22626875607708996 1105 92-93 0.12577720379124813 1105 94-95 -0.2011844436734016 1105 96-97 0.11977002248348256 1105 98-99 -0.12299737021001533 1105 100-101 -0.12166330561056071 1106 1 0.11451647664474507 1106 2 0.015242636417411859 1106 3 -0.16255798254550768 1106 4 -0.011933566589860334 1106 5 0.0053760565018379225 1106 6 -0.1834089431097894 1106 7 -0.08313909449809387 1106 8 -0.2493863381875272 1106 9 -0.2188995508727558 1106 10-11 -0.2287173531811746 1106 12-13 -0.49296549720345695 1106 14-15 -0.052672554782553505 1106 16-17 -0.09701293343336204 1106 18-19 -0.1344974860049959 1106 20-21 0.1288854921623468 1106 22-23 0.12393846397431929 1106 24-25 0.13164388798859505 1106 26-27 0.20515842028287778 1106 28-29 0.1683543592932253 1106 30-31 0.16972156551855733 1106 32-33 0.16329096509890917 1106 34-35 -0.348810361638499 1106 36-37 -0.031467528838305725 1106 38-39 0.006883359476653084 1106 40-41 -0.06740448571920155 1106 42-43 -0.35236840942134506 1106 44-45 0.13928675996219653 1106 46-47 0.004647037287512035 1106 48-49 -0.05774611993484058 1106 50-51 0.2244149670211968 1106 52-53 0.3733146155357119 1106 54-55 0.21122718006720476 1106 56-57 0.2520946372736361 1106 58-59 0.23659112301681517 1106 60-61 0.3450398556823373 1106 62-63 0.10406297849295498 1106 64-65 -0.3526345700109985 1106 66-67 -0.20158964991573924 1106 68-69 0.09006544255178994 1106 70-71 -0.02830131676613945 1106 72-73 -0.16426350612810126 1106 74-75 -0.2676125528953541 1106 76-77 -0.48980103261423125 1106 78-79 -0.28056361574955346 1106 80-81 -0.5458185144781673 1106 82-83 -0.5130184141626799 1106 84-85 -0.2939964165444451 1106 86-87 -0.24352022011905916 1106 88-89 0.100131528262839 1106 90-91 0.006929039552758809 1106 92-93 -0.18861144116115014 1106 94-95 -0.23344406426195619 1106 96-97 -0.3274243941161217 1106 98-99 -0.8195646896224957 1106 100-101 -1.2504017696107113 1107 1 0.35218805457081714 1107 2 0.1660259708909706 1107 3 0.16178104983895736 1107 4 0.0704393864473829 1107 5 0.16413889180568475 1107 6 0.1332833267876552 1107 7 0.08072054589571565 1107 8 0.02266301903662793 1107 9 0.16196457371442818 1107 10-11 0.1785714823590112 1107 12-13 0.21392778396977263 1107 14-15 0.14904928840128662 1107 16-17 0.20844242054425877 1107 18-19 0.12439626784325952 1107 20-21 0.3012688316114307 1107 22-23 0.26304796909600014 1107 24-25 0.15057732166170723 1107 26-27 -0.0018701330205601607 1107 28-29 0.18681667278871572 1107 30-31 0.3863342269899448 1107 32-33 0.32471104259273886 1107 34-35 0.23432913346992734 1107 36-37 0.45582932973856316 1107 38-39 0.426439977859971 1107 40-41 0.4087798451035667 1107 42-43 0.5214366867618025 1107 44-45 0.6287883987762868 1107 46-47 0.6258306528783208 1107 48-49 0.8049993134646272 1107 50-51 0.9887631419358414 1107 52-53 1.1021903952940235 1107 54-55 0.9934996896606378 1107 56-57 1.0981549530951895 1107 58-59 1.2222149607648944 1107 60-61 1.2385542567222174 1107 62-63 1.0494114830362307 1107 64-65 1.2046555094108484 1107 66-67 1.044412825408763 1107 68-69 1.2687661459668647 1107 70-71 1.110431914154269 1107 72-73 1.265614220305011 1107 74-75 0.8777474575532516 1107 76-77 0.9328015409769144 1107 78-79 1.1372857750594072 1107 80-81 1.030655329800286 1107 82-83 1.3117886286952647 1107 84-85 1.4191582539202052 1107 86-87 1.2860950472843982 1107 88-89 0.8330203812173664 1107 90-91 1.22084869723653 1107 92-93 1.0338459092465335 1107 94-95 0.952362313119302 1107 96-97 1.0024203694530733 1107 98-99 1.1841685102008697 1107 100-101 1.3041626541300104 1108 1 0.08311288721011323 1108 2 0.0031655523574158906 1108 3 0.059486758552253605 1108 4 0.008502746312444742 1108 5 0.00926434644698304 1108 6 0.07210442214372392 1108 7 0.027132174170453993 1108 8 0.08357177450550068 1108 9 0.1985862594452783 1108 10-11 0.0853502499516523 1108 12-13 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86-87 0.010486411547148577 2316 88-89 0.08949084832648779 2316 90-91 0.11585606263972537 2316 92-93 0.4059076677563418 2316 94-95 0.4960142067407034 2316 96-97 0.2965870493193883 2316 98-99 0.11355171708158451 2316 100-101 0.24815589063073418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 14.0 6 38.0 7 94.0 8 193.0 9 426.0 10 747.0 11 1211.0 12 1962.0 13 2679.0 14 3542.0 15 4978.0 16 7031.0 17 9407.0 18 8573.0 19 5419.0 20 4362.0 21 4287.0 22 4503.0 23 4983.0 24 5727.0 25 6498.0 26 7580.0 27 9305.0 28 11388.0 29 13682.0 30 17343.0 31 21867.0 32 27506.0 33 36242.0 34 49554.0 35 72073.0 36 109291.0 37 181056.0 38 178039.0 39 17630.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8917305240507 21.197716452709752 15.138693051536617 21.771859971702927 2 14.795805772790585 22.363624304328688 40.851387121865336 21.989182801015396 3 18.627891972721848 26.56002218912612 30.7973011269423 24.01478471120973 4 11.956080001447116 18.748243863319807 38.62897731041261 30.666698824820465 5 12.19707542310833 39.48721598276567 34.97092430670062 13.344784287425382 6 29.2399621337739 39.08903989821944 18.104638612697247 13.566359355309412 7 26.69918659969731 34.78917315356925 21.43843421949146 17.073206027241977 8 24.86918665999385 37.06416154648562 20.329942657992003 17.736709135528532 9 25.863476577809667 17.487684432036758 21.357998637298234 35.29084035285534 10-11 23.471392307367633 28.757288344076166 27.98422642556091 19.787092922995292 12-13 24.78381492712629 26.400537379923144 28.540891705765198 20.27475598718537 14-15 21.433128124114393 28.08817765772067 27.619251478772604 22.859442739392332 16-17 19.727459646541693 31.31681610158761 28.413356889180992 20.54236736268971 18-19 19.609579914017136 29.652390456263905 31.400447400314746 19.33758222940421 20-21 21.036135715448577 28.49958093905829 31.38253932841716 19.08174401707598 22-23 20.332429080248595 29.016816213123146 31.02398069533223 19.62677401129603 24-25 19.91986590049865 28.974054399536925 31.51060917592721 19.595470524037214 26-27 20.090433239008938 29.160589245089273 31.325789391490062 19.423188124411727 28-29 19.64753878918413 29.649697428686512 31.29924773222258 19.40351604990678 30-31 20.071511694513617 29.283074158712548 31.388207203024475 19.25720694374936 32-33 19.78142504838797 29.334386512870296 31.151543289899724 19.732645148842003 34-35 20.046307741472564 29.480786508046574 31.20177030636671 19.271135444114154 36-37 19.926136740489728 29.424228355050136 31.0733386796264 19.576296224833733 38-39 19.78269127569386 29.740785181522728 31.092452682291512 19.3840708604919 40-41 20.19504830080418 29.547031856642995 30.995664052395565 19.26225579015726 42-43 19.881165884127995 29.770520986227883 30.606810197083963 19.741502932560163 44-45 20.085259305263286 29.91962471434515 30.28309224767406 19.712023732717505 46-47 20.135305432115143 29.941331468160413 29.90967578551315 20.01368731421129 48-49 20.12433146213076 29.972685668115794 29.86463427134648 20.038348598406966 50-51 20.321031023190667 30.346783690258906 29.3262641629117 20.00592112363873 52-53 20.344956496047562 30.482673789697735 29.066549289405298 20.105820424849412 54-55 20.233565093547234 30.37986284924572 29.062803179080436 20.323768878126607 56-57 20.42647741593155 30.436848420532176 28.832236941277202 20.304437222259068 58-59 20.424246444011647 30.38469191483717 29.008242536795965 20.182819104355218 60-61 20.47200130240523 30.309080055714 29.096094593209404 20.122824048671365 62-63 20.398921897893842 30.21531893853974 29.22965142571165 20.15610773785477 64-65 20.288639529204627 30.19481811549199 29.222837916875193 20.29370443842819 66-67 20.1667199286089 30.193310702032594 29.083794099380754 20.55617526997775 68-69 20.432868848999377 30.155444475932637 29.038451102522206 20.37323557254578 70-71 20.363286643713785 30.05866853183959 29.031637593685744 20.54640723076088 72-73 20.166539038993772 30.01905370612673 29.034411234451028 20.779996020428467 74-75 20.105724077883927 30.398174700989106 28.685147979942936 20.810953241184034 76-77 19.886582211315247 30.089600656026338 29.04936477596821 20.9744523566902 78-79 19.883386494781334 30.057161118380193 28.823373350135967 21.236079036702506 80-81 19.81573377872376 30.077481051812814 28.795697239021507 21.311087930441914 82-83 19.839791614535127 30.05200592113792 28.825389742924934 21.28281272140202 84-85 19.754797801344374 29.803107446149095 28.824312558336974 21.61778219416956 86-87 19.630804295525394 29.747236911128933 28.713573353753763 21.908385439591914 88-89 19.64159737589465 29.725771343467173 28.612094279667406 22.020537000970776 90-91 19.604952119063128 29.628711677159465 28.766131172057797 22.000205031719613 92-93 19.29344827606999 29.689064903412763 28.838872946952243 22.178613873565002 94-95 19.009629357178603 29.954958485833327 28.69096215186286 22.344450005125204 96-97 19.009991136408857 29.859388472507792 28.742093616405484 22.388526774677867 98-99 19.141923743438166 29.96380966852261 28.261139591450835 22.63312699658839 100-101 19.01821769567378 29.89678971008969 28.13375966729547 22.95123292694106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 763.0 1 521.0 2 329.5 3 412.0 4 407.0 5 427.5 6 706.5 7 1314.0 8 1818.5 9 1938.5 10 1764.5 11 1537.0 12 1524.0 13 1572.0 14 1562.0 15 1548.0 16 1525.5 17 1529.5 18 1643.0 19 1805.5 20 2027.0 21 2371.5 22 2851.0 23 3473.5 24 4250.0 25 5290.5 26 6617.5 27 8313.0 28 9965.0 29 11968.5 30 14309.0 31 16542.0 32 19325.5 33 22195.0 34 25307.0 35 27919.5 36 30160.5 37 31998.0 38 32699.0 39 32968.5 40 32978.5 41 32922.5 42 32606.0 43 32089.5 44 31818.0 45 31568.5 46 30784.5 47 30330.0 48 30932.0 49 29397.5 50 26295.0 51 23784.0 52 21091.0 53 18522.0 54 16261.0 55 14241.5 56 11905.5 57 9900.5 58 8391.0 59 6913.5 60 5541.5 61 4506.5 62 3503.0 63 2303.5 64 1462.5 65 1078.5 66 805.5 67 624.0 68 470.0 69 306.5 70 211.0 71 164.0 72 136.0 73 130.5 74 109.0 75 85.5 76 73.0 77 50.0 78 35.5 79 29.0 80 14.5 81 12.0 82 11.0 83 7.5 84 5.0 85 2.0 86 1.5 87 3.0 88 2.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020983195354754684 2 0.0 3 0.0 4 0.0 5 0.02315387073628103 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003195716533913788 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.632619221330504E-4 24-25 0.0 26-27 5.426688453815867E-4 28-29 0.0010853376907631735 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.426688453815867E-4 42-43 0.011576935368140515 44-45 0.0 46-47 0.0 48-49 0.0 50-51 6.029653837573185E-5 52-53 0.0 54-55 1.8088961512719554E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.205930767514637E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.0148269187865923E-4 84-85 1.205930767514637E-4 86-87 0.0 88-89 0.0 90-91 0.011456342291389051 92-93 0.0012662273058903688 94-95 0.0 96-97 0.0 98-99 9.647446140117096E-4 100-101 0.01820955458947102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 829235.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.80736727032804 #Duplication Level Percentage of deduplicated Percentage of total 1 83.97481125523666 61.979597357717296 2 10.82048771584503 15.97263421774894 3 2.791087605119335 6.180084838641093 4 1.0176621953924765 3.004438696498434 5 0.4943087354665854 1.8241813191756848 6 0.26319879455058376 1.165560605670153 7 0.14935376120567717 0.7716385534558602 8 0.09883518861556707 0.5835812052305043 9 0.06813244934395384 0.45258090405806 >10 0.2950954298741977 3.7887185496852083 >50 0.017036106108039593 0.8655186554946179 >100 0.008844282258434377 1.2797980761029628 >500 3.2756599528393923E-4 0.22145933886780098 >1k 6.551319905678785E-4 1.2415987278991176 >5k 1.6378299764196962E-4 0.6686089537542615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5531 0.6670003075123456 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4577 0.5519545122914493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3203 0.3862596248349382 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1420 0.17124216898707847 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1071 0.12915518520081762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 981 0.1183018082931859 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 0.10262470831549561 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.029653837573185E-5 0.0 16-17 0.0 0.0 0.0 1.205930767514637E-4 0.0 18-19 0.0 0.0 0.0 1.205930767514637E-4 0.0 20-21 0.0 0.0 0.0 1.8088961512719554E-4 0.0 22-23 0.0 0.0 0.0 3.617792302543911E-4 0.0 24-25 0.0 0.0 0.0 3.617792302543911E-4 0.0 26-27 0.0 0.0 0.0 0.0012662273058903688 0.0 28-29 0.0 0.0 0.0 0.004160461147925497 0.0 30-31 0.0 0.0 0.0 0.008320922295850994 0.0 32-33 0.0 0.0 0.0 0.013506424596163934 0.0 34-35 0.0 0.0 0.0 0.01887281651160407 0.0 36-37 0.0 0.0 0.0 0.024721580734050057 0.0 38-39 0.0 0.0 0.0 0.03515288187305167 0.0 40-41 0.0 0.0 0.0 0.05625667030455782 0.0 42-43 0.0 0.0 0.0 0.09478615832665047 0.0 44-45 0.0 0.0 0.0 0.13150675019747116 0.0 46-47 0.0 0.0 0.0 0.2099525466242983 0.0 48-49 0.0 0.0 0.0 0.2932823626595597 0.0 50-51 0.0 0.0 0.0 0.3488154745036087 0.0 52-53 0.0 0.0 0.0 0.4231611063208861 0.0 54-55 0.0 0.0 0.0 0.5076365565852865 0.0 56-57 0.0 0.0 0.0 0.670979879045144 0.0 58-59 0.0 0.0 0.0 0.8746012891399905 0.0 60-61 0.0 0.0 0.0 1.0741828311636628 0.0 62-63 0.0 0.0 0.0 1.2923357070070607 0.0 64-65 0.0 0.0 0.0 1.5514902289459562 0.0 66-67 0.0 0.0 0.0 1.8801063630936947 0.0 68-69 0.0 0.0 0.0 2.2284394652902977 0.0 70-71 0.0 0.0 0.0 2.4860262772314243 0.0 72-73 0.0 0.0 0.0 2.770927421056757 0.0 74-75 0.0 0.0 0.0 3.1100351528818733 0.0 76-77 0.0 0.0 0.0 3.5136601807690218 0.0 78-79 0.0 0.0 0.0 3.924460496722883 0.0 80-81 0.0 0.0 0.0 4.430830826002279 0.0 82-83 0.0 0.0 0.0 4.996774135196898 0.0 84-85 0.0 0.0 0.0 5.661784656942845 0.0 86-87 0.0 0.0 0.0 6.335417583676521 0.0 88-89 0.0 0.0 0.0 7.202783288211424 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5685 0.0 32.354992 1 GGTATCA 3685 0.0 31.729149 1 ATGGACG 80 4.4843293E-4 29.69681 5 TATCAAC 6715 0.0 27.171589 2 TGCGTAA 35 0.008394597 27.141548 60-61 ATCAACG 6705 0.0 26.99952 3 TCAACGC 6760 0.0 26.850136 4 CAACGCA 6805 0.0 26.602757 5 AACGCAG 6930 0.0 26.113459 6 GAGTACT 4125 0.0 23.83665 12-13 GTACTAG 180 1.938115E-7 23.764616 1 GTGTCGG 50 0.0016544181 23.748854 64-65 TACATGG 4045 0.0 23.728085 2 GTACATG 4170 0.0 23.36569 1 ACGCAGA 7800 0.0 23.080406 7 CGCAGAG 7780 0.0 22.895521 8 GTACTTT 4340 0.0 22.490273 14-15 ACATGGG 4130 0.0 21.744108 3 GCAGAGT 8240 0.0 21.559727 9 AGTACTT 4290 0.0 21.148275 12-13 >>END_MODULE