FastQCFastQC Report
Thu 26 May 2016
SRR937790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780558
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.9144740044942208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53670.6875850353208858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.49272443559607354No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.16091052810937817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.13003517996100228No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9150.11722383218159317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8730.11184306611424136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.039.13211
GTATCAA51500.034.6148571
CTATACG753.0690175E-431.681654
TCAACGC63200.027.82174
ATCAACG63450.027.7085233
TATCAAC64200.027.5363852
CAACGCA63750.027.5071265
AACGCAG64850.027.1033846
GTACATG34800.027.047331
TACATGG34900.025.4633312
CGCAGAG71200.024.7528768
ACGCAGA71450.024.6662677
ACCGCCC1000.001652324223.7520878
GCAGAGT74650.023.545279
ACATGGG35800.023.3600033
GAGTACT47750.023.0293412-13
AGTACTT49050.022.27371812-13
GTATAGG2606.184564E-1021.9404931
AGAGTAC64700.021.87841610-11
GTAGCCC4350.021.8466073
Produced by FastQC (version 0.11.5)