##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937790_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 780558 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874434699279234 33.0 31.0 34.0 30.0 34.0 2 31.998184632019658 33.0 31.0 34.0 30.0 34.0 3 32.02408789609485 34.0 31.0 34.0 30.0 34.0 4 35.60297761345089 37.0 35.0 37.0 33.0 37.0 5 35.60141591015658 37.0 35.0 37.0 33.0 37.0 6 35.68529180406837 37.0 35.0 37.0 33.0 37.0 7 35.635517155675814 37.0 35.0 37.0 33.0 37.0 8 35.62078410572949 37.0 35.0 37.0 33.0 37.0 9 37.27468170206442 39.0 37.0 39.0 34.0 39.0 10-11 37.17636421636829 39.0 37.0 39.0 34.0 39.0 12-13 37.06715964732922 39.0 37.0 39.0 33.0 39.0 14-15 38.222303787803085 40.0 38.0 41.0 33.5 41.0 16-17 38.19474850043174 40.0 38.0 41.0 33.0 41.0 18-19 38.19698984572575 40.0 38.0 41.0 33.5 41.0 20-21 38.00739535050566 40.0 38.0 41.0 33.0 41.0 22-23 37.93123252852446 40.0 38.0 41.0 33.0 41.0 24-25 37.823967597539195 40.0 37.5 41.0 33.0 41.0 26-27 37.89287791554247 40.0 38.0 41.0 33.0 41.0 28-29 37.82442688435709 40.0 38.0 41.0 33.0 41.0 30-31 37.58652976460429 40.0 37.0 41.0 33.0 41.0 32-33 37.313127916183035 39.5 36.0 41.0 32.5 41.0 34-35 37.054976055591 39.0 36.0 41.0 31.5 41.0 36-37 37.06339375164946 39.5 36.0 41.0 31.5 41.0 38-39 37.00650752410455 40.0 36.0 41.0 31.0 41.0 40-41 36.805424452763276 40.0 36.0 41.0 31.0 41.0 42-43 36.538742156252326 39.0 35.0 41.0 30.0 41.0 44-45 36.242621944813834 39.0 35.0 41.0 30.0 41.0 46-47 35.833395468370064 39.0 35.0 41.0 28.5 41.0 48-49 35.45264733690514 39.0 35.0 41.0 26.5 41.0 50-51 34.53286687216068 38.0 34.0 40.0 25.0 40.5 52-53 34.61078741105722 38.5 34.5 40.0 24.0 40.5 54-55 34.93061309985933 39.0 35.0 40.0 24.0 41.0 56-57 34.71606786427146 39.0 35.0 40.0 23.0 41.0 58-59 34.53299818847542 39.0 35.0 40.0 22.5 41.0 60-61 34.27909777364398 38.0 34.0 40.0 22.0 41.0 62-63 33.96425826139762 38.0 34.0 40.0 19.5 41.0 64-65 33.6547123980537 38.0 34.0 40.0 18.0 41.0 66-67 33.286046008112145 37.5 33.0 40.0 14.5 41.0 68-69 32.87045677579373 37.0 33.0 39.5 10.0 41.0 70-71 32.47039553755134 36.0 32.0 39.0 7.5 41.0 72-73 32.02808888000636 36.0 32.0 39.0 4.5 40.0 74-75 31.57059180739932 35.0 31.0 38.5 2.0 40.0 76-77 31.129434327750147 35.0 31.0 37.5 2.0 39.5 78-79 30.610176309768143 35.0 30.5 37.0 2.0 39.0 80-81 30.074526812869767 35.0 30.0 36.5 2.0 39.0 82-83 29.55381342578002 34.0 29.0 36.0 2.0 37.5 84-85 29.075487023385833 34.0 29.0 35.5 2.0 37.0 86-87 28.573074006031582 34.0 29.0 35.0 2.0 37.0 88-89 28.1102192277832 34.0 28.0 35.0 2.0 36.0 90-91 27.623358546065763 34.0 26.5 35.0 2.0 36.0 92-93 27.115959352155762 33.5 26.0 35.0 2.0 35.5 94-95 26.55324011796689 33.0 25.0 35.0 2.0 35.0 96-97 25.870541330689072 32.5 24.0 35.0 2.0 35.0 98-99 25.065520435380844 32.0 18.5 34.5 2.0 35.0 100-101 22.69195562661583 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23028720326691854 1101 2 0.3226654967598428 1101 3 0.22661490979566068 1101 4 0.1846884532625026 1101 5 0.13670279734773771 1101 6 0.2026193210993057 1101 7 0.34435946015267405 1101 8 0.3217762515543612 1101 9 0.3659058113277993 1101 10-11 0.2154398172009948 1101 12-13 0.3350891637359794 1101 14-15 0.48676223593955825 1101 16-17 0.5871513466042089 1101 18-19 0.648179960714991 1101 20-21 0.6243985579026443 1101 22-23 0.5699730864849144 1101 24-25 0.4730064308632862 1101 26-27 0.5922558074451061 1101 28-29 0.5629260250518513 1101 30-31 0.6010339711746369 1101 32-33 0.6424802913668799 1101 34-35 0.7010415490819497 1101 36-37 0.7379289772122846 1101 38-39 0.8656565456746037 1101 40-41 1.020614495510351 1101 42-43 1.0520697467874385 1101 44-45 1.1807025922046464 1101 46-47 1.2238843873350405 1101 48-49 1.3244508896947025 1101 50-51 1.1967484047596884 1101 52-53 1.103773494046841 1101 54-55 1.4143745724357117 1101 56-57 1.4037798725734802 1101 58-59 1.3516149977408602 1101 60-61 1.4385788541416744 1101 62-63 1.581475773035784 1101 64-65 1.6433250689218255 1101 66-67 1.6411686723631504 1101 68-69 1.6969360058855045 1101 70-71 1.7800192571345832 1101 72-73 1.84385221824434 1101 74-75 1.7477372531248072 1101 76-77 1.7323865893134247 1101 78-79 1.6602345936841836 1101 80-81 1.7358888569205355 1101 82-83 1.7538532692448499 1101 84-85 1.8163928058889738 1101 86-87 1.9685954055906798 1101 88-89 2.0002638501256698 1101 90-91 2.0281733724831845 1101 92-93 2.056556170802672 1101 94-95 2.1392377227024006 1101 96-97 2.300941021760419 1101 98-99 2.419454312480404 1101 100-101 2.640946433734843 1102 1 0.24129645058759053 1102 2 0.20331407890290976 1102 3 0.18229134477561004 1102 4 0.18410457196297614 1102 5 0.16451271492480402 1102 6 0.1468671514393165 1102 7 0.08687138367048419 1102 8 0.16498579251457102 1102 9 0.22767616088760434 1102 10-11 0.10671562019425807 1102 12-13 0.1288015758184784 1102 14-15 0.3723362547417892 1102 16-17 0.28005683115415536 1102 18-19 0.16482638973988628 1102 20-21 0.4582854458779124 1102 22-23 0.45839542669546773 1102 24-25 0.3653985827117694 1102 26-27 0.38791590626679096 1102 28-29 0.3417905949915365 1102 30-31 0.47408986104371564 1102 32-33 0.38120771626257977 1102 34-35 0.47880497075154693 1102 36-37 0.5382755505533154 1102 38-39 0.727818980915643 1102 40-41 0.7128811084086735 1102 42-43 0.8229996112876421 1102 44-45 0.9483308001987254 1102 46-47 1.0844031475395468 1102 48-49 1.1273236661620487 1102 50-51 1.0085602429753848 1102 52-53 1.1612368472243944 1102 54-55 1.274239260403462 1102 56-57 1.4682989797089903 1102 58-59 1.4712804865112048 1102 60-61 1.5056851902820583 1102 62-63 1.4771622046358317 1102 64-65 1.3663650428841123 1102 66-67 1.3582795239714613 1102 68-69 1.3255611219017354 1102 70-71 1.4309317840261997 1102 72-73 1.35862450453752 1102 74-75 1.129164865255941 1102 76-77 1.2289537159915263 1102 78-79 1.1697962088089433 1102 80-81 1.3374142356386436 1102 82-83 1.3475262045136063 1102 84-85 1.5197824469584518 1102 86-87 1.5056023477873275 1102 88-89 1.6363061810728112 1102 90-91 1.7087480529579473 1102 92-93 1.7334451564586857 1102 94-95 1.7791821332684172 1102 96-97 1.9606517814498936 1102 98-99 2.3201938209471287 1102 100-101 2.6337639337214327 1103 1 0.13139391563150937 1103 2 0.20717444122158568 1103 3 0.2604915646523729 1103 4 0.12069880372583697 1103 5 0.13134455471976736 1103 6 0.16410904092278855 1103 7 0.1798421555380969 1103 8 0.09369314017707353 1103 9 0.1741352572077446 1103 10-11 0.1223752960239679 1103 12-13 0.18166020142934514 1103 14-15 0.37182127421431943 1103 16-17 0.31351150262503324 1103 18-19 0.2271088744839389 1103 20-21 0.3099645100131809 1103 22-23 0.29893014150437125 1103 24-25 0.2451779434303063 1103 26-27 0.22891751778596614 1103 28-29 0.26922101856159486 1103 30-31 0.20398798025515674 1103 32-33 0.4042703083046817 1103 34-35 0.2578362155590668 1103 36-37 0.4717400664033349 1103 38-39 0.5932190041324361 1103 40-41 0.5399332915846813 1103 42-43 0.6184425173028387 1103 44-45 0.6251295901936587 1103 46-47 0.886505804497645 1103 48-49 1.0136134050185532 1103 50-51 1.0190208916346108 1103 52-53 1.1094579736034547 1103 54-55 1.2544654307464995 1103 56-57 1.4069921127708511 1103 58-59 1.4469594641525134 1103 60-61 1.4225152660553206 1103 62-63 1.4198428802902825 1103 64-65 1.394580285397268 1103 66-67 1.494439237357767 1103 68-69 1.6314737812042779 1103 70-71 1.7276866100481385 1103 72-73 1.7545495006111516 1103 74-75 1.7617291040976504 1103 76-77 1.7464121249122435 1103 78-79 1.59287860341718 1103 80-81 1.752612875862436 1103 82-83 1.4701897293248578 1103 84-85 1.7443782583742902 1103 86-87 1.7557568419455727 1103 88-89 1.846914723034903 1103 90-91 1.9991358987346466 1103 92-93 1.9364285723785848 1103 94-95 1.905278102877169 1103 96-97 2.119891727095265 1103 98-99 2.209634899208236 1103 100-101 2.2868215523604256 1104 1 0.06705791769208957 1104 2 0.09458984587348951 1104 3 0.14323347877914117 1104 4 0.110315183888396 1104 5 0.1445687280842236 1104 6 0.02951469046814026 1104 7 0.101012260435418 1104 8 0.17114357106691358 1104 9 0.19741927084094613 1104 10-11 0.16497679683750022 1104 12-13 0.11834497072960204 1104 14-15 0.44910210628246006 1104 16-17 0.3203185826049335 1104 18-19 0.3236827062020069 1104 20-21 0.38768883846533697 1104 22-23 0.14050851701974665 1104 24-25 0.18387023675101233 1104 26-27 0.28193347458633156 1104 28-29 0.25242484006744803 1104 30-31 0.34535131897575155 1104 32-33 0.2994917934824741 1104 34-35 0.34035737913394826 1104 36-37 0.293068161219054 1104 38-39 0.4042818355076818 1104 40-41 0.5446711752453339 1104 42-43 0.6503112242342581 1104 44-45 0.6758949615606511 1104 46-47 0.6816136493954374 1104 48-49 0.5120357731985479 1104 50-51 0.48147243806751305 1104 52-53 0.6588955766551479 1104 54-55 0.7802158828588688 1104 56-57 0.8436093146261072 1104 58-59 0.9879384393402972 1104 60-61 0.9075864884909777 1104 62-63 0.9214440926951255 1104 64-65 0.8502138685034026 1104 66-67 0.9423374816925332 1104 68-69 0.996514385157468 1104 70-71 1.1405477331007035 1104 72-73 0.8901777690162511 1104 74-75 0.40535300465288415 1104 76-77 0.6245825798905571 1104 78-79 0.8444444449447666 1104 80-81 1.0271534625836658 1104 82-83 1.0831139757891286 1104 84-85 1.2787301822779291 1104 86-87 1.0918441155039353 1104 88-89 1.3046338274805684 1104 90-91 1.4187614753252937 1104 92-93 1.4362734846860192 1104 94-95 1.3410327994948048 1104 96-97 1.0911193323417656 1104 98-99 1.5008737143019886 1104 100-101 1.685355202761933 1105 1 -0.11781994047349542 1105 2 -0.005459436614309254 1105 3 -0.016670697401377055 1105 4 0.08102418100435216 1105 5 0.18209194771790038 1105 6 0.12677032660590726 1105 7 0.2052549285718115 1105 8 0.06761389513572169 1105 9 -0.053205508627208076 1105 10-11 -0.005277430812597572 1105 12-13 0.08522582667017531 1105 14-15 -0.02429701479790225 1105 16-17 0.018031029495034545 1105 18-19 -0.1604863881375067 1105 20-21 0.16570914051741426 1105 22-23 0.10363267681164956 1105 24-25 0.023229739376986913 1105 26-27 0.14276949804362715 1105 28-29 0.18967204860819464 1105 30-31 0.14830229019069208 1105 32-33 0.28398676462742856 1105 34-35 0.4780319455792892 1105 36-37 0.3466256861300394 1105 38-39 0.548827518040973 1105 40-41 0.652303836995415 1105 42-43 0.5620263131322858 1105 44-45 0.669574263790615 1105 46-47 0.8071272516536894 1105 48-49 1.0082572061233321 1105 50-51 0.8762412040515102 1105 52-53 0.7694443619438829 1105 54-55 0.8875459967918147 1105 56-57 1.1075811915306986 1105 58-59 1.1896372271077453 1105 60-61 1.0883810446715572 1105 62-63 1.4595848014881128 1105 64-65 1.187594052277511 1105 66-67 1.3973351342130798 1105 68-69 1.4564548670760473 1105 70-71 1.5623657319984119 1105 72-73 1.4760255298707925 1105 74-75 1.4523346709841967 1105 76-77 1.4515726154964277 1105 78-79 1.4591793255732526 1105 80-81 1.5034147589172235 1105 82-83 1.5715027008890807 1105 84-85 1.7863828194742517 1105 86-87 1.695794442255579 1105 88-89 1.2943953177363419 1105 90-91 1.3208307565883253 1105 92-93 1.4266415286378304 1105 94-95 1.6629232640624458 1105 96-97 1.8702105807634126 1105 98-99 2.102009981838826 1105 100-101 2.275496955527146 1106 1 0.046787470156886 1106 2 0.330916616656463 1106 3 0.16350392631434474 1106 4 0.15171247116769138 1106 5 0.1893541053278014 1106 6 0.1285488599550888 1106 7 0.06761483823218839 1106 8 0.16923027468774876 1106 9 0.2942028341071605 1106 10-11 0.002401885943520199 1106 12-13 -0.13562966357032025 1106 14-15 -0.18987891816501445 1106 16-17 -0.16944500067499035 1106 18-19 0.039011002375033854 1106 20-21 0.055271863534954946 1106 22-23 0.08099394336064591 1106 24-25 0.19864628175564292 1106 26-27 -0.021442059956065407 1106 28-29 0.2546670135421607 1106 30-31 0.522409939678333 1106 32-33 0.47894250314489994 1106 34-35 0.19650222747570467 1106 36-37 0.4618748714361658 1106 38-39 0.3440954530807687 1106 40-41 0.21925900135835974 1106 42-43 0.4372878786453356 1106 44-45 0.345055847111432 1106 46-47 0.4767831850696993 1106 48-49 0.2597626796550401 1106 50-51 0.18653808231056956 1106 52-53 0.18624578297632866 1106 54-55 0.28673814343446935 1106 56-57 0.3332239193926654 1106 58-59 0.25799525730916884 1106 60-61 0.1478194946337652 1106 62-63 0.48609795657936417 1106 64-65 0.23147986729658498 1106 66-67 0.7166031769068582 1106 68-69 0.790197883585229 1106 70-71 0.6299421494984614 1106 72-73 0.6883090724939116 1106 74-75 0.6457518548011123 1106 76-77 0.7975691212278768 1106 78-79 0.47278521783405125 1106 80-81 0.445704379504253 1106 82-83 0.3140953225548877 1106 84-85 0.298555568216603 1106 86-87 0.026813588963275237 1106 88-89 0.381536723472955 1106 90-91 0.43758149054171724 1106 92-93 0.5240246399722253 1106 94-95 0.5919669603616171 1106 96-97 0.342253068198179 1106 98-99 0.46947672685366015 1106 100-101 0.2011506730655448 1107 1 0.030182541412738573 1107 2 0.14512834343186043 1107 3 0.12960380455344378 1107 4 0.18023624884257572 1107 5 0.19306695539039964 1107 6 0.2347123625532248 1107 7 0.1932257864237812 1107 8 0.3538480997979292 1107 9 0.2627079189110546 1107 10-11 0.28562031814328037 1107 12-13 0.3365368230274086 1107 14-15 0.27763578746515094 1107 16-17 0.09387366990174684 1107 18-19 0.4331047411207294 1107 20-21 0.3393327611357506 1107 22-23 0.30421511946806845 1107 24-25 0.18068440492280047 1107 26-27 0.18791380771898503 1107 28-29 0.04721044445570044 1107 30-31 0.14360705514538807 1107 32-33 0.39169695770750224 1107 34-35 0.3655848190964903 1107 36-37 0.21105598649165103 1107 38-39 0.30421661375371656 1107 40-41 0.294997284015146 1107 42-43 -0.20113368846892854 1107 44-45 -0.16977197268244026 1107 46-47 -0.1119929463634719 1107 48-49 -0.2300205516392282 1107 50-51 -0.3649637784018225 1107 52-53 -0.2976316157991974 1107 54-55 -0.1559420541268821 1107 56-57 -0.0056615396116370675 1107 58-59 -0.10593907755046672 1107 60-61 0.022669437183196806 1107 62-63 -0.26350975037130553 1107 64-65 -0.35163909825021733 1107 66-67 -0.2678187160464489 1107 68-69 -0.364245531893836 1107 70-71 -0.20891544169359832 1107 72-73 -0.40053565663095014 1107 74-75 -0.18582366268272565 1107 76-77 0.048139339540032466 1107 78-79 0.15796039918920002 1107 80-81 -0.0753701175889212 1107 82-83 0.063246426120255 1107 84-85 -0.049494113661598504 1107 86-87 0.1289619829198152 1107 88-89 0.3169841519169836 1107 90-91 0.27117635257461004 1107 92-93 0.5203761002455849 1107 94-95 0.6361695979428639 1107 96-97 0.4443285771073029 1107 98-99 0.19256713861130592 1107 100-101 0.607038927425787 1108 1 0.03868175476522495 1108 2 0.05926975757327213 1108 3 0.13774959655927788 1108 4 0.14011152530927262 1108 5 0.3062871938928069 1108 6 -0.07909159120558229 1108 7 0.08545507707293609 1108 8 0.09483015511407444 1108 9 -0.11646758328927831 1108 10-11 0.23362747777571968 1108 12-13 0.1767105695217026 1108 14-15 -0.04303708117611649 1108 16-17 0.051300648491256595 1108 18-19 0.28849840689814243 1108 20-21 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-0.3824471790583033 2316 66-67 -0.22817225139998243 2316 68-69 -0.46677078441909003 2316 70-71 -0.42886493664309455 2316 72-73 -0.39422252531781865 2316 74-75 -0.30299537985444047 2316 76-77 -0.2346889432882513 2316 78-79 -0.15567596444716258 2316 80-81 -0.17259233981114264 2316 82-83 -0.21705660418277617 2316 84-85 -0.08661532578281239 2316 86-87 -0.17078549182765812 2316 88-89 -0.23124817131533604 2316 90-91 9.743323725892594E-4 2316 92-93 0.2309821608516458 2316 94-95 0.15132111309437946 2316 96-97 0.06124776902649387 2316 98-99 -0.1965742755301072 2316 100-101 -0.04599137560451538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2655.0 3 439.0 4 389.0 5 442.0 6 518.0 7 624.0 8 744.0 9 958.0 10 1265.0 11 1842.0 12 2754.0 13 3718.0 14 4640.0 15 6017.0 16 8142.0 17 10989.0 18 9358.0 19 6080.0 20 5353.0 21 5286.0 22 5612.0 23 6051.0 24 6730.0 25 7450.0 26 8370.0 27 9678.0 28 11428.0 29 13632.0 30 16435.0 31 20743.0 32 26364.0 33 34756.0 34 47292.0 35 68344.0 36 98728.0 37 149876.0 38 152466.0 39 24385.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.743105950653124 19.943871456644874 15.470542147783759 21.842480444918248 2 14.167661749033808 20.743551224272306 43.34578791263819 21.7429991140557 3 18.12057529793315 25.961847095649315 31.701088505596598 24.216489100820937 4 11.220049587264985 17.36812573748538 40.49438323431716 30.917441440932475 5 11.819474030901745 37.82401183372689 36.52327774717479 13.833236388196577 6 28.605091715566232 38.07697638959478 19.48654170966235 13.831390185176629 7 25.753138451192626 34.098941743261726 22.931419932598054 17.21649987294759 8 24.199257200204308 35.374107754737466 21.883975390585896 18.542659654472327 9 25.37233359858305 16.775547398413636 22.65395179300254 35.19816721000077 10-11 22.92712072272811 27.35021726895079 29.656521887015014 20.06614012130609 12-13 24.407948060248984 25.30760132891218 29.984270988219606 20.30017962261923 14-15 21.21990381542419 26.578529559365805 29.420364074149656 22.781202551060353 16-17 19.27197361497889 29.821875441140612 30.17498042939825 20.731170514482244 18-19 18.96622531259906 27.85413883338274 33.82191575129653 19.35772010272166 20-21 20.41613251359283 26.784392079263824 33.8192361835613 18.98023922358204 22-23 19.57374040707374 27.126164626164627 33.73026873026873 19.569826236492904 24-25 19.288727414596085 27.007837435142562 34.15810135663217 19.54533379362918 26-27 19.338964627311775 27.24780131850306 33.885939346805834 19.52729470737933 28-29 18.964662004901335 27.548671640727925 34.05073696490714 19.435929389463606 30-31 19.303039033689824 27.380242719723718 34.05615805208214 19.26056019450432 32-33 19.035732344946364 27.389043894751165 33.86968641472724 19.70553734557523 34-35 19.365161664573684 27.575605708745165 33.7396310658305 19.319601560850653 36-37 19.224108448492807 27.481198158988406 33.64055358677079 19.654139805748 38-39 19.089665544286124 27.768267769884613 33.72319375436291 19.41887293146635 40-41 19.524440115845625 27.586605125283427 33.472045250111314 19.416909508759638 42-43 19.19765828068165 27.747089693829952 33.208385172276394 19.846866853212003 44-45 19.44739959165498 27.838106983315857 32.93196089024861 19.78253253478055 46-47 19.536326958021498 27.857148356894267 32.46821327009353 20.13831141499071 48-49 19.538644077150188 27.992964412014405 32.361715466807155 20.10667604402825 50-51 19.660827276524778 28.257378055862308 31.926498246001287 20.155296421611627 52-53 19.721526655637426 28.355008913662612 31.757730628987563 20.1657338017124 54-55 19.739756438718288 28.13697434778703 31.729439090430787 20.393830123063893 56-57 19.866628514089722 28.297556738057324 31.35525908893186 20.480555658921094 58-59 19.91132982801708 28.13199151794737 31.540660278393545 20.416018375642007 60-61 20.0036440483324 28.046148536029246 31.66524026981355 20.284967145824805 62-63 19.965624847627872 27.78112818249241 31.92924287411688 20.324004095762835 64-65 19.90472493736547 27.83128935710184 31.85593897263328 20.40804673289941 66-67 19.841661908397437 27.81534493709461 31.761543347298048 20.5814498072099 68-69 19.966380731796512 27.668657731469303 31.839692550509103 20.525268986225083 70-71 19.842279106055784 27.587875085975917 31.82712937963885 20.742716428329448 72-73 19.775639174927022 27.391973823501107 31.962275045712634 20.870111955859237 74-75 19.711991930692243 27.598238817117505 31.801773245778946 20.887996006411303 76-77 19.46684459899464 27.596598327393345 31.8662042992471 21.070352774364913 78-79 19.522085147991408 27.417868810807622 31.756430754701004 21.303615286499973 80-81 19.52587514973191 27.50165370754495 31.65276440233309 21.31970674039005 82-83 19.567090347003884 27.240744649252857 31.819584411352057 21.372580592391202 84-85 19.83157740773933 27.113381493786637 31.691853501725987 21.363187596748045 86-87 19.55970315855921 27.17011416386325 31.545433317167177 21.724749360410364 88-89 19.731800151787027 26.922245395868387 31.51434823639001 21.831606215954576 90-91 19.652770515583367 26.889851098460817 31.83274833015864 21.624630055797176 92-93 19.587636802690408 26.973490479065237 31.83881934121714 21.60005337702722 94-95 19.48319233115826 27.166404807011897 31.508869760695134 21.84153310113471 96-97 19.45235360916679 27.201203590240397 31.62696401419159 21.71947878640123 98-99 19.817015857128652 27.058876367007677 31.56630448381726 21.55780329204641 100-101 20.135740800381853 26.672701587085694 31.61269668558407 21.57886092694838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1132.0 1 726.0 2 440.0 3 559.5 4 429.5 5 382.0 6 712.5 7 1561.5 8 2563.5 9 2800.5 10 2173.5 11 1580.0 12 1414.5 13 1443.0 14 1482.0 15 1508.0 16 1551.5 17 1610.0 18 1747.0 19 1913.5 20 2174.0 21 2521.5 22 2977.5 23 3607.0 24 4402.5 25 5440.0 26 6721.5 27 8447.5 28 10129.0 29 11848.5 30 13999.0 31 16230.5 32 18835.5 33 21447.0 34 23979.0 35 26511.5 36 28822.5 37 30321.5 38 30691.5 39 30739.0 40 30877.5 41 30661.5 42 30072.0 43 29683.5 44 29592.0 45 29313.5 46 28467.0 47 28071.5 48 28815.0 49 27484.0 50 24327.5 51 21763.5 52 19028.0 53 16609.5 54 14560.5 55 12639.5 56 10725.5 57 8897.5 58 7412.5 59 6027.0 60 4728.0 61 3710.5 62 2861.5 63 2001.0 64 1342.5 65 967.5 66 702.0 67 531.0 68 412.0 69 283.5 70 199.5 71 162.0 72 126.0 73 110.0 74 95.0 75 81.5 76 59.5 77 33.0 78 27.0 79 18.5 80 13.0 81 14.0 82 8.5 83 6.0 84 5.5 85 3.0 86 3.0 87 3.0 88 1.5 89 2.0 90 1.5 91 1.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2546895938546527 2 0.2213800896281891 3 0.2167680044276018 4 0.22150820310598315 5 0.2263765152621586 6 0.18064000368966815 7 0.1732094219776109 8 0.17218451415525815 9 0.1816649115120209 10-11 0.1863410534515052 12-13 0.1824976491176825 14-15 0.18333038672334406 16-17 0.1701346985105527 18-19 0.17487489718893406 20-21 0.16488204592099498 22-23 0.1714158332884936 24-25 0.17282508154422863 26-27 0.1718001737218759 28-29 0.17711688305033066 30-31 0.17250479784974337 32-33 0.17628414544466908 34-35 0.17519518088341932 36-37 0.16814893960474428 38-39 0.1616792089761427 40-41 0.15950127985364318 42-43 0.16673969134900932 44-45 0.1684692232992295 46-47 0.16680374808790635 48-49 0.17634820218356612 50-51 0.17147989002739067 52-53 0.1815367980342268 54-55 0.16501015939878907 56-57 0.15770769116452588 58-59 0.16212760614842203 60-61 0.15437674074187951 62-63 0.15578598899761453 64-65 0.15828420181459932 66-67 0.15540164856423225 68-69 0.15924505289805496 70-71 0.16270411679849545 72-73 0.15796391812011407 74-75 0.16770054243246496 76-77 0.15828420181459932 78-79 0.16091052810937817 80-81 0.15994967702592247 82-83 0.1482913505466602 84-85 0.14726644272430747 86-87 0.1475867264187927 88-89 0.15072550662474793 90-91 0.15597815921430566 92-93 0.15328777618062975 94-95 0.15328777618062975 96-97 0.15668278334217317 98-99 0.1608464713704811 100-101 0.15437674074187951 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 780558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.45513842910368 #Duplication Level Percentage of deduplicated Percentage of total 1 86.17542843462978 65.88554310156937 2 9.747247754081528 14.904543526821461 3 2.281338561156436 5.232601664906025 4 0.7672848084899861 2.346514649906008 5 0.34865424312049087 1.332820421083575 6 0.1840802274625845 0.8444327563627676 7 0.10897850651755757 0.5832376761117789 8 0.06784113385773029 0.4149442624224097 9 0.05691514693637731 0.3916309893960152 >10 0.2373164394568752 3.1570369506672433 >50 0.014309836329349233 0.712800380893309 >100 0.008416593643151917 1.143227582792248 >500 0.0013466549650271548 0.6540450894523507 >1k 5.049956118851831E-4 0.7872478969264126 >5k 3.366637412567887E-4 1.6093730506890291 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7138 0.9144740044942208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5367 0.6875850353208858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3846 0.49272443559607354 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1256 0.16091052810937817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 0.13003517996100228 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 915 0.11722383218159317 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 873 0.11184306611424136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.405673889704545E-5 0.0 16-17 0.0 0.0 0.0 2.562269555881818E-4 0.0 18-19 0.0 0.0 0.0 3.8434043338227266E-4 0.0 20-21 0.0 0.0 0.0 3.8434043338227266E-4 0.0 22-23 0.0 0.0 0.0 8.327376056615908E-4 0.0 24-25 0.0 0.0 0.0 0.0012170780390438635 0.0 26-27 0.0 0.0 0.0 0.0017935886891172724 0.0 28-29 0.0 0.0 0.0 0.003587177378234545 0.0 30-31 0.0 0.0 0.0 0.008455489534409999 0.0 32-33 0.0 0.0 0.0 0.013580028646173635 0.0 34-35 0.0 0.0 0.0 0.01896079471352545 0.0 36-37 0.0 0.0 0.0 0.02421344730308318 1.281134777940909E-4 38-39 0.0 0.0 0.0 0.03599988726013954 1.281134777940909E-4 40-41 0.0 0.0 0.0 0.0555371926237384 1.281134777940909E-4 42-43 0.0 0.0 0.0 0.10287512266865499 1.281134777940909E-4 44-45 0.0 0.0 0.0 0.1471383292465134 1.281134777940909E-4 46-47 0.0 0.0 0.0 0.24091739499178794 1.281134777940909E-4 48-49 0.0 0.0 0.0 0.33636193594838565 1.281134777940909E-4 50-51 0.0 0.0 0.0 0.40029056136763697 1.281134777940909E-4 52-53 0.0 0.0 0.0 0.48516574040622223 1.281134777940909E-4 54-55 0.0 0.0 0.0 0.5733718698674537 1.281134777940909E-4 56-57 0.0 0.0 0.0 0.7625314198304289 1.281134777940909E-4 58-59 0.0 0.0 0.0 0.9909577507372931 1.281134777940909E-4 60-61 0.0 0.0 0.0 1.2133627481878348 1.281134777940909E-4 62-63 0.0 0.0 0.0 1.451653816884844 1.281134777940909E-4 64-65 0.0 0.0 0.0 1.742215184521842 1.281134777940909E-4 66-67 0.0 0.0 0.0 2.1177157879363224 1.281134777940909E-4 68-69 0.0 0.0 0.0 2.5153800230091807 1.281134777940909E-4 70-71 0.0 0.0 0.0 2.8059413906461788 1.281134777940909E-4 72-73 0.0 0.0 0.0 3.1002821058781027 1.281134777940909E-4 74-75 0.0 0.0 0.0 3.471234680830893 1.281134777940909E-4 76-77 0.0 0.0 0.0 3.923859597877416 1.281134777940909E-4 78-79 0.0 0.0 0.0 4.351950783926371 1.281134777940909E-4 80-81 0.0 0.0 0.0 4.893678624778683 1.281134777940909E-4 82-83 0.0 0.0 0.0 5.499783488222528 1.281134777940909E-4 84-85 0.0 0.0 0.0 6.189546452665914 1.281134777940909E-4 86-87 0.0 0.0 0.0 6.881038949059519 1.281134777940909E-4 88-89 0.0 0.0 0.0 7.830872273424909 1.281134777940909E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3365 0.0 39.1321 1 GTATCAA 5150 0.0 34.614857 1 CTATACG 75 3.0690175E-4 31.68165 4 TCAACGC 6320 0.0 27.8217 4 ATCAACG 6345 0.0 27.708523 3 TATCAAC 6420 0.0 27.536385 2 CAACGCA 6375 0.0 27.507126 5 AACGCAG 6485 0.0 27.103384 6 GTACATG 3480 0.0 27.04733 1 TACATGG 3490 0.0 25.463331 2 CGCAGAG 7120 0.0 24.752876 8 ACGCAGA 7145 0.0 24.666267 7 ACCGCCC 100 0.0016523242 23.752087 8 GCAGAGT 7465 0.0 23.54527 9 ACATGGG 3580 0.0 23.360003 3 GAGTACT 4775 0.0 23.02934 12-13 AGTACTT 4905 0.0 22.273718 12-13 GTATAGG 260 6.184564E-10 21.940493 1 AGAGTAC 6470 0.0 21.878416 10-11 GTAGCCC 435 0.0 21.846607 3 >>END_MODULE