FastQCFastQC Report
Thu 26 May 2016
SRR937790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780558
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.6646527227957435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.5387171741241522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.3970236676838877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12740.1632165707096718No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10420.1334942438614427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.12350139259350362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.10723098091365407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.035.7009241
GTATCAA51150.033.4331171
CTACACG804.4941079E-429.68554
TAGGACG2002.3646862E-1128.4980774
ATCAACG62600.027.1627043
TCAACGC63050.026.9688384
TATCAAC63700.026.7682112
GTAGGAC3400.026.5423283
CAACGCA64150.026.4323565
AACGCAG65550.025.867826
GGACGTG2451.0913936E-1125.2023816
AGGACGT2151.6607373E-924.3006865
ACGCAGA72100.023.3216957
CGCAGAG72350.023.1754578
GTACATG39600.022.7917881
TACATGG39250.022.750062
GAGTACT40400.022.6329712-13
AGTACTT42000.022.61897312-13
GTAGCCC3800.022.4984823
TGTAGGA6750.021.8133432
Produced by FastQC (version 0.11.5)