##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937790_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 780558 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22491089707619 33.0 31.0 34.0 31.0 34.0 2 32.43476205483769 34.0 31.0 34.0 31.0 34.0 3 32.513098321969665 34.0 31.0 34.0 31.0 34.0 4 36.12491576538835 37.0 35.0 37.0 35.0 37.0 5 36.04386861706625 37.0 35.0 37.0 35.0 37.0 6 36.08279717842876 37.0 35.0 37.0 35.0 37.0 7 36.03114566758652 37.0 35.0 37.0 35.0 37.0 8 36.01253718493693 37.0 35.0 37.0 35.0 37.0 9 37.696110987268085 39.0 37.0 39.0 35.0 39.0 10-11 37.626562664145396 39.0 37.0 39.0 35.0 39.0 12-13 37.53755390374578 39.0 37.0 39.0 35.0 39.0 14-15 38.75178526131306 40.0 38.0 41.0 35.0 41.0 16-17 38.73843455579214 40.0 38.0 41.0 34.5 41.0 18-19 38.746298161059144 40.0 38.0 41.0 35.0 41.0 20-21 38.66318133950328 40.0 38.0 41.0 34.0 41.0 22-23 38.50696681092244 40.0 38.0 41.0 34.0 41.0 24-25 38.57033686157851 40.0 38.0 41.0 34.0 41.0 26-27 38.43733008950007 40.0 38.0 41.0 34.0 41.0 28-29 38.29894255135429 40.0 38.0 41.0 34.0 41.0 30-31 38.30259762887576 40.0 38.0 41.0 34.0 41.0 32-33 38.21067941139544 40.0 38.0 41.0 34.0 41.0 34-35 38.076639404118595 40.0 37.0 41.0 34.0 41.0 36-37 37.913976924200384 40.0 37.0 41.0 33.0 41.0 38-39 37.75696617035506 40.0 37.0 41.0 33.0 41.0 40-41 37.56914348453286 40.0 37.0 41.0 33.0 41.0 42-43 37.34483792363925 40.0 37.0 41.0 33.0 41.0 44-45 36.995897806441036 40.0 36.0 41.0 31.5 41.0 46-47 36.634604987713914 40.0 36.0 41.0 31.0 41.0 48-49 36.30682729534512 40.0 36.0 41.0 30.0 41.0 50-51 36.05718088854384 39.0 36.0 41.0 29.5 41.0 52-53 35.819229961130375 39.0 35.0 41.0 28.5 41.0 54-55 35.6111076947517 39.0 35.0 40.5 28.0 41.0 56-57 35.365823551869305 39.0 35.0 40.0 27.5 41.0 58-59 35.14227065765773 38.5 35.0 40.0 27.0 41.0 60-61 34.8134840716513 38.0 34.5 40.0 26.0 41.0 62-63 34.49158166337415 38.0 34.0 40.0 26.0 41.0 64-65 34.14307521029827 38.0 34.0 40.0 24.5 41.0 66-67 33.74855039599876 37.0 33.5 40.0 23.0 41.0 68-69 33.32550559984011 36.5 33.0 39.0 22.0 40.5 70-71 32.920750283771355 36.0 32.5 39.0 21.5 40.0 72-73 32.42308323020198 35.5 32.0 38.5 19.5 40.0 74-75 31.91569620707238 35.0 31.0 38.0 18.0 40.0 76-77 30.612694380174183 34.0 30.0 36.0 16.5 39.0 78-79 31.183067754093866 35.0 31.0 37.0 16.0 39.0 80-81 30.962211648589854 35.0 31.0 36.0 12.0 39.0 82-83 30.586767415105605 34.0 31.0 36.0 7.5 37.5 84-85 30.155706558641384 34.0 30.5 35.5 4.5 37.0 86-87 29.70839002354726 34.0 30.0 35.0 2.0 36.5 88-89 29.330350211002898 34.0 29.5 35.0 2.0 36.0 90-91 28.814413534932704 34.0 29.0 35.0 2.0 36.0 92-93 28.356890199062722 34.0 29.0 35.0 2.0 35.0 94-95 27.90279710156068 34.0 28.5 35.0 2.0 35.0 96-97 27.381124144522254 33.0 27.0 35.0 2.0 35.0 98-99 26.56057077116627 33.0 25.5 35.0 2.0 35.0 100-101 24.042473461293078 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36080884817211256 1101 2 0.3048357831368378 1101 3 0.31034449283190924 1101 4 0.20939743982460612 1101 5 0.19845869353446943 1101 6 0.2256114035881609 1101 7 0.2160530524115174 1101 8 0.18083835931948755 1101 9 0.31325343840991593 1101 10-11 0.39339597393998105 1101 12-13 0.30672272425328373 1101 14-15 0.33858652286641444 1101 16-17 0.3047873802147478 1101 18-19 0.30929428116402136 1101 20-21 0.4729892923943808 1101 22-23 0.3711132909909054 1101 24-25 0.32141734509666975 1101 26-27 0.3453030331892464 1101 28-29 0.38542270406330914 1101 30-31 0.5054388850596112 1101 32-33 0.6074763681251198 1101 34-35 0.44544647031901263 1101 36-37 0.48440618141010106 1101 38-39 0.3502157258429932 1101 40-41 0.3130363995606942 1101 42-43 0.5305133528251886 1101 44-45 0.4757606801764567 1101 46-47 0.568536693353586 1101 48-49 0.5837535578003639 1101 50-51 0.6012934632090108 1101 52-53 0.5405453149327641 1101 54-55 0.5349892978567041 1101 56-57 0.5746070126733827 1101 58-59 0.6129705868544093 1101 60-61 0.773340111001545 1101 62-63 0.7459835518112499 1101 64-65 0.9463652816178367 1101 66-67 0.734725827132614 1101 68-69 0.800263884740211 1101 70-71 0.7438105237464328 1101 72-73 0.744327111480068 1101 74-75 0.6669364130122197 1101 76-77 0.7009835138431093 1101 78-79 0.8277670524908309 1101 80-81 0.9525745583112872 1101 82-83 1.0845710602199112 1101 84-85 0.9534916239910309 1101 86-87 0.834108526702213 1101 88-89 1.0572723077225348 1101 90-91 0.9991479060151711 1101 92-93 1.2062407636726284 1101 94-95 1.180354318211716 1101 96-97 1.5179152907070623 1101 98-99 1.7063058710863146 1101 100-101 1.9147705624328637 1102 1 0.22836704405936814 1102 2 0.2208690920896217 1102 3 0.19231373757742176 1102 4 0.09792171337075928 1102 5 0.03761549025256983 1102 6 0.05116367661212706 1102 7 0.14683405847566178 1102 8 0.10692461995765967 1102 9 0.16693260591857495 1102 10-11 0.141718097586903 1102 12-13 0.14314945117387623 1102 14-15 0.23506025536114805 1102 16-17 0.24168617471892873 1102 18-19 0.23077543512091125 1102 20-21 0.22596391278212025 1102 22-23 0.25792928701370954 1102 24-25 0.2311158101952273 1102 26-27 0.2797516274927858 1102 28-29 0.22402289111992246 1102 30-31 0.25719723509867976 1102 32-33 0.25388370982890507 1102 34-35 0.373548820329475 1102 36-37 0.438837733175248 1102 38-39 0.4565241475036501 1102 40-41 0.3854139856720096 1102 42-43 0.6449060268584716 1102 44-45 0.700269298908232 1102 46-47 0.7640215830778132 1102 48-49 0.7146147245972827 1102 50-51 0.4726439815489698 1102 52-53 0.4852596691135531 1102 54-55 0.5271791030925002 1102 56-57 0.35914855408425694 1102 58-59 0.5058174150287869 1102 60-61 0.5815840589332808 1102 62-63 0.6364713832957776 1102 64-65 0.7309519777080666 1102 66-67 0.7095518698480348 1102 68-69 0.7773418494355084 1102 70-71 0.6348509412081498 1102 72-73 0.748964631173763 1102 74-75 0.8261417532460165 1102 76-77 0.7740495970457495 1102 78-79 0.8614511020697719 1102 80-81 0.9183338990708556 1102 82-83 1.094009282359476 1102 84-85 0.9827614683549193 1102 86-87 0.9927275267183084 1102 88-89 0.9727653252890462 1102 90-91 0.9155218869053563 1102 92-93 0.8588611210115609 1102 94-95 1.0567757964596787 1102 96-97 1.213150712004694 1102 98-99 0.9816631295445468 1102 100-101 0.9157281992962503 1103 1 0.2030152070868425 1103 2 0.2299811177100466 1103 3 0.16359887859672995 1103 4 0.14855027413391042 1103 5 0.09129808470739675 1103 6 0.07731046142652076 1103 7 0.17515514229059193 1103 8 0.07437955773890792 1103 9 0.10066684637635603 1103 10-11 0.0469320904898467 1103 12-13 0.11839595897995281 1103 14-15 0.07887434340745614 1103 16-17 0.16275018708301303 1103 18-19 0.06131867413788683 1103 20-21 0.04665252994844593 1103 22-23 0.12189533130317898 1103 24-25 0.10236754861015385 1103 26-27 0.02297749965925533 1103 28-29 0.0052500584197403555 1103 30-31 0.2165666940789137 1103 32-33 0.26466501210939697 1103 34-35 0.2479991284073222 1103 36-37 0.20613112071146134 1103 38-39 0.1946192772104567 1103 40-41 0.2992859108683845 1103 42-43 0.37149831250707877 1103 44-45 0.42157378726226824 1103 46-47 0.43650648646632817 1103 48-49 0.233692050203814 1103 50-51 0.4372437648588132 1103 52-53 0.45712096238045064 1103 54-55 0.5780634470016182 1103 56-57 0.6033779269802295 1103 58-59 0.49109722301218284 1103 60-61 0.4436270350541136 1103 62-63 0.5254176819230238 1103 64-65 0.7214769050417473 1103 66-67 0.7032503291173455 1103 68-69 0.6214791517797167 1103 70-71 0.6313513497783347 1103 72-73 0.632512454370989 1103 74-75 0.7889484938574434 1103 76-77 0.7203556218057834 1103 78-79 0.7794781519198857 1103 80-81 0.6423953826244819 1103 82-83 0.8809615536227646 1103 84-85 0.823536602249856 1103 86-87 0.7759030844336863 1103 88-89 0.772623691418012 1103 90-91 0.833142642726056 1103 92-93 0.8485612259424009 1103 94-95 0.9748369886652064 1103 96-97 0.9382535373984133 1103 98-99 0.8941602558962991 1103 100-101 1.292890832992022 1104 1 0.12370009610391008 1104 2 0.16317278614526032 1104 3 0.12392998628563845 1104 4 0.009499193145479978 1104 5 0.017468180535267663 1104 6 0.08412797128048055 1104 7 0.18475606810062573 1104 8 0.03914472232231958 1104 9 0.0015776011183206151 1104 10-11 0.22373976905382875 1104 12-13 0.3299596056992655 1104 14-15 0.18335356619137855 1104 16-17 0.1981704928687833 1104 18-19 0.20234462910622852 1104 20-21 0.17137477786745592 1104 22-23 0.19350956744641223 1104 24-25 0.1811434804513823 1104 26-27 0.24915612077548843 1104 28-29 0.12347132048891041 1104 30-31 0.3462526551056513 1104 32-33 0.2750422833278421 1104 34-35 0.33581866800548 1104 36-37 0.3380912477564024 1104 38-39 0.4411388735834052 1104 40-41 0.5237744389138683 1104 42-43 0.4645318821904212 1104 44-45 0.5420642271490053 1104 46-47 0.36575792987020606 1104 48-49 0.533817117997998 1104 50-51 0.6480451238260585 1104 52-53 0.7408892668094254 1104 54-55 0.4637951072017614 1104 56-57 0.6610891409994366 1104 58-59 0.6812995807078863 1104 60-61 0.6526530480669734 1104 62-63 0.6804976654528971 1104 64-65 0.5718984533429463 1104 66-67 0.8956075894887263 1104 68-69 0.6740584142878632 1104 70-71 0.717808203656709 1104 72-73 0.8452143177813625 1104 74-75 0.7235577182194497 1104 76-77 0.8172641164393895 1104 78-79 0.8853132583318093 1104 80-81 0.8438688063984259 1104 82-83 1.0862142195892694 1104 84-85 0.8848783193880472 1104 86-87 0.9115023305805288 1104 88-89 0.7966441448545183 1104 90-91 0.9509077111732402 1104 92-93 1.0257013000579889 1104 94-95 0.924239855701547 1104 96-97 1.0327268831750374 1104 98-99 1.3893239686412429 1104 100-101 1.4773182147671307 1105 1 0.08004135254489597 1105 2 0.14626072768281517 1105 3 0.14996659530054046 1105 4 0.06343289799295349 1105 5 -0.007014134633529068 1105 6 0.0906702944394624 1105 7 0.15882006534780402 1105 8 -0.004483401939538112 1105 9 0.29095328822992883 1105 10-11 0.12668024222403318 1105 12-13 0.057779570064234065 1105 14-15 0.21562484733193088 1105 16-17 0.08085119775416416 1105 18-19 0.3163657884986222 1105 20-21 0.2864625094441138 1105 22-23 -8.45692219812122E-4 1105 24-25 0.07969664575767865 1105 26-27 0.05310659333330392 1105 28-29 0.03380197973696397 1105 30-31 0.07865312051897178 1105 32-33 0.1255107585743218 1105 34-35 0.1371417132549695 1105 36-37 0.19724175180835601 1105 38-39 0.16736882158891575 1105 40-41 0.08791822130105942 1105 42-43 0.061927492258050165 1105 44-45 0.2688453225033314 1105 46-47 0.36868644693389996 1105 48-49 0.3775588252716915 1105 50-51 0.4369892246539493 1105 52-53 0.6472532690140156 1105 54-55 0.5801724944133966 1105 56-57 0.530326516371602 1105 58-59 0.6058747744113901 1105 60-61 0.5515444837037506 1105 62-63 0.39919016314186706 1105 64-65 0.6354765077636486 1105 66-67 0.7579862422979673 1105 68-69 0.5864595212483508 1105 70-71 0.7492403702490193 1105 72-73 0.6469463493571439 1105 74-75 0.4922099734035861 1105 76-77 0.504926143553508 1105 78-79 0.8184407133433176 1105 80-81 0.7681968586320309 1105 82-83 0.8563959815694346 1105 84-85 0.7823700157262685 1105 86-87 1.0318288780943874 1105 88-89 0.9070019240514782 1105 90-91 0.8254896119608865 1105 92-93 0.718764716996585 1105 94-95 1.0138093192223003 1105 96-97 1.1218166443736841 1105 98-99 1.190589420450845 1105 100-101 0.9183775258037841 1106 1 0.049205217591669737 1106 2 0.04534858453891388 1106 3 0.03919962802510213 1106 4 0.015087292854268242 1106 5 0.031472160791395254 1106 6 -0.03651838933372176 1106 7 0.02276114273980312 1106 8 -0.13029725476310006 1106 9 0.09026385424311911 1106 10-11 -0.07702910999061174 1106 12-13 -0.07268246733206496 1106 14-15 0.04729776764781235 1106 16-17 0.04416137800728137 1106 18-19 0.11564944101851182 1106 20-21 0.11776312643594622 1106 22-23 0.18083608712093735 1106 24-25 0.15568689410194025 1106 26-27 0.29582481130120186 1106 28-29 0.1438691961864933 1106 30-31 0.24446378857916073 1106 32-33 0.2609764629340887 1106 34-35 0.273971034608806 1106 36-37 0.20008561093415977 1106 38-39 0.2913020940678521 1106 40-41 0.20252148276788517 1106 42-43 0.11654618412770645 1106 44-45 0.23750904513287452 1106 46-47 0.43451573016029243 1106 48-49 0.23986939352766967 1106 50-51 0.3939758553761479 1106 52-53 0.29308515387809564 1106 54-55 0.4118297151915087 1106 56-57 0.43773849242757024 1106 58-59 0.08115697360263852 1106 60-61 0.10978752600503583 1106 62-63 0.1717229247129879 1106 64-65 0.15649482463862796 1106 66-67 0.22563622334639177 1106 68-69 0.45406868349859764 1106 70-71 0.46752898606562354 1106 72-73 0.5088386370713565 1106 74-75 0.3768856545953163 1106 76-77 0.15174149231873812 1106 78-79 0.06386822297049477 1106 80-81 -0.127193757081411 1106 82-83 0.053463307915723846 1106 84-85 -0.0044268972960495034 1106 86-87 0.1666172595393256 1106 88-89 -0.08686964924405771 1106 90-91 0.30259632171904727 1106 92-93 0.2551282106371495 1106 94-95 0.3881804966652034 1106 96-97 0.389944564207358 1106 98-99 0.19440104394004365 1106 100-101 0.3768129995821923 1107 1 -0.06275050259834103 1107 2 -0.03097063481935436 1107 3 -0.12474885510843592 1107 4 -0.02103796653074852 1107 5 -0.26171437348172333 1107 6 -0.020410793854310327 1107 7 -0.06131390313490925 1107 8 -0.0801609168508719 1107 9 -0.030330887743808432 1107 10-11 -0.0334931562254539 1107 12-13 -0.12452731650289195 1107 14-15 -0.11964328978560701 1107 16-17 -0.10490998074148905 1107 18-19 -0.027010177235332833 1107 20-21 -0.07599962844734875 1107 22-23 0.04418519792336184 1107 24-25 0.04740543777294448 1107 26-27 -0.2838728443726879 1107 28-29 -0.16233375008436468 1107 30-31 -0.25582775161051785 1107 32-33 -0.2607711490087681 1107 34-35 -0.24345921011345695 1107 36-37 -0.28278640292257506 1107 38-39 -0.046987422489912944 1107 40-41 -0.13467261440073486 1107 42-43 -0.19143172741556924 1107 44-45 -0.005129565077893972 1107 46-47 -0.18741452313668816 1107 48-49 -0.43317900214381666 1107 50-51 -0.1684308946367139 1107 52-53 -0.6750833735009607 1107 54-55 -0.2474648432965978 1107 56-57 -0.30352387659371516 1107 58-59 -0.7672421277675952 1107 60-61 -0.5954739169474124 1107 62-63 -0.5002000400792497 1107 64-65 -0.49385845347355684 1107 66-67 -0.48195111008984526 1107 68-69 -0.46823371227579 1107 70-71 -0.45203593307484624 1107 72-73 -0.22951476108679003 1107 74-75 -0.1748970279174138 1107 76-77 -0.3799313481401825 1107 78-79 -0.03200562587448985 1107 80-81 -0.47851897873770355 1107 82-83 -0.6247363456659336 1107 84-85 -0.14683784891752794 1107 86-87 -0.10298024350469248 1107 88-89 -0.29783541899590915 1107 90-91 -0.17135799221829373 1107 92-93 0.09262885374051066 1107 94-95 0.21643331345791594 1107 96-97 -0.06371769505761904 1107 98-99 -0.04761161233407307 1107 100-101 0.477610876483638 1108 1 -0.10598761411988278 1108 2 -0.06629566845138868 1108 3 -0.16022779803659404 1108 4 -0.07433618574319212 1108 5 -0.05552151943334138 1108 6 -0.03648006443825125 1108 7 -0.23397733200601323 1108 8 -0.23369399024850424 1108 9 -0.2210416066712355 1108 10-11 -0.22425802842374054 1108 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0.4998541289938281 2316 92-93 0.2956591567708138 2316 94-95 0.3068373538619582 2316 96-97 0.28678735544743006 2316 98-99 0.7271358624134017 2316 100-101 1.1773583512311099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 6.0 5 11.0 6 43.0 7 113.0 8 224.0 9 373.0 10 653.0 11 1213.0 12 1878.0 13 2572.0 14 3432.0 15 4953.0 16 6844.0 17 9185.0 18 8201.0 19 5164.0 20 4327.0 21 4073.0 22 4288.0 23 4694.0 24 5320.0 25 6103.0 26 7343.0 27 8900.0 28 10743.0 29 13361.0 30 16266.0 31 20477.0 32 26147.0 33 33788.0 34 46473.0 35 67144.0 36 102886.0 37 168015.0 38 167442.0 39 17900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88015567792559 21.204773883836175 15.174496910918345 21.74057352731989 2 14.812608416030585 22.464954558149426 40.79133132963854 21.93110569618145 3 18.641664040340373 26.59059288355253 30.81321311164577 23.954529964461322 4 11.926739495001646 18.82220004432732 38.693907043303696 30.557153417367346 5 12.155485453189586 39.58364832416336 35.0134410324353 13.24742519021175 6 29.200008199262577 39.23103728358431 18.031715772562706 13.537238744590407 7 26.58367475575166 34.91399742235683 21.46643811222228 17.035889709669235 8 24.92255540267347 37.07270952318726 20.216050568951953 17.788684505187316 9 25.812431619431226 17.455077009011504 21.45542035313199 35.27707101842528 10-11 23.422794974876947 28.798628673333692 27.965762954194307 19.812813397595054 12-13 24.792946372005336 26.404342152717142 28.58374613806308 20.218965337214435 14-15 21.414936494149057 28.172345937137276 27.590902918168798 22.821814650544866 16-17 19.73722644569654 31.397218400170136 28.308018110121218 20.557537044012104 18-19 19.579454697793118 29.672106364933803 31.457752018427843 19.29068691884524 20-21 21.03418323814502 28.522095731515147 31.471075820118426 18.972645210221405 22-23 20.273731114205805 29.02470959468168 31.124070683764078 19.577488607348435 24-25 19.8898736544882 29.079773700352824 31.52456319709746 19.505789448061513 26-27 20.03532106683202 29.290734529577712 31.354332948136836 19.319611455453433 28-29 19.614782719543296 29.743071040610648 31.327153475161314 19.314992764684742 30-31 19.947012265584362 29.41914630302937 31.44891218846005 19.184929242926213 32-33 19.750614304126024 29.503573084895677 31.181090963131503 19.5647216478468 34-35 19.977375159821563 29.585117313511617 31.187496637021205 19.250010889645612 36-37 19.92375966936474 29.47859095672583 31.044137655369617 19.553511718539816 38-39 19.75631535388786 29.849223247984135 31.129461231580486 19.265000166547523 40-41 20.066427306299065 29.704344038357444 31.006882945093377 19.22234571025011 42-43 19.80218825558436 29.84653509114742 30.619305288433818 19.731971364834408 44-45 20.109139871732786 30.023073237350715 30.24759210718486 19.620194783731638 46-47 20.136556155980724 30.059329351566443 29.91558602948147 19.88852846297136 48-49 20.08601538899095 30.115250884623563 29.814568552240832 19.98416517414465 50-51 20.257174995323858 30.422338890896 29.36738845800056 19.953097655779583 52-53 20.300157067123774 30.605348993924856 28.991183230458212 20.103310708493154 54-55 20.17697595822476 30.493377814332824 29.030514068140995 20.299132159301422 56-57 20.396690572641624 30.499463204528045 28.785753268815384 20.31809295401495 58-59 20.3538366143195 30.43720005432011 29.01699809623372 20.191965235126666 60-61 20.34256262827362 30.387363911458216 29.174193333487068 20.0958801267811 62-63 20.35313199019163 30.277506604249783 29.291801506102043 20.077559899456542 64-65 20.235587874315552 30.33451710186815 29.19885517796243 20.231039845853864 66-67 20.171979532590786 30.20448192190715 29.084321728814515 20.53921681668755 68-69 20.275572267257697 30.201938998728473 29.10298088225403 20.4195078517598 70-71 20.25454866902908 30.129727707614297 29.07125415407952 20.544469469277107 72-73 20.112214595199845 30.05612651462159 29.089766551620766 20.7418923385578 74-75 19.94207989668929 30.353349783103884 28.820728248253175 20.883842071953655 76-77 19.798144404387628 30.08520827408085 29.113851885446053 21.002795436085467 78-79 19.724222927700442 30.091037437320484 28.86396654700868 21.3207730879704 80-81 19.77649322664043 30.107371905739228 28.73534061530341 21.38079425231693 82-83 19.644094831175654 29.99955159535965 28.854262086106502 21.50209148735819 84-85 19.663970728595093 29.872321944457614 28.855996423067115 21.60771090388018 86-87 19.458067177583217 29.872796127898248 28.660650457749455 22.00848623676908 88-89 19.553896058973198 29.726042139085116 28.575262824799697 22.144798977141996 90-91 19.515509264301336 29.57490944547445 28.85066543322028 22.05891585700393 92-93 19.105640799629487 29.736854074088786 28.851437440785922 22.306067685495808 94-95 18.87995510903738 29.917059334476104 28.6705792522785 22.532406304208017 96-97 18.89731448527848 29.80688174357319 28.79882084355038 22.49698292759795 98-99 18.99150146724122 30.024732671275828 28.262890167337883 22.72087569414506 100-101 18.771319031375977 29.93570013557046 28.05783143645743 23.23514939659613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 664.0 1 460.0 2 307.5 3 392.5 4 386.5 5 412.0 6 677.0 7 1253.5 8 1763.0 9 1840.5 10 1633.5 11 1459.0 12 1488.0 13 1471.0 14 1444.0 15 1444.0 16 1439.0 17 1502.5 18 1594.0 19 1733.5 20 1958.0 21 2251.0 22 2670.5 23 3260.0 24 4054.0 25 5141.0 26 6388.5 27 7932.5 28 9408.5 29 11189.5 30 13452.5 31 15754.0 32 18494.0 33 21157.5 34 23816.5 35 26273.0 36 28677.0 37 30359.0 38 30772.0 39 31032.0 40 31405.0 41 31172.0 42 30674.0 43 30465.0 44 30107.0 45 29605.5 46 28697.5 47 28337.0 48 29258.5 49 27672.5 50 24426.5 51 22216.5 52 19604.0 53 17122.0 54 15093.5 55 13134.5 56 11057.0 57 9257.0 58 7771.5 59 6530.0 60 5265.0 61 4056.0 62 3102.0 63 2107.0 64 1354.0 65 1004.0 66 752.0 67 588.5 68 429.0 69 284.0 70 195.5 71 145.0 72 128.5 73 115.5 74 88.0 75 66.5 76 50.5 77 41.0 78 34.0 79 20.5 80 14.5 81 11.5 82 10.0 83 5.0 84 3.0 85 3.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029337986414846813 2 0.0 3 0.0 4 1.281134777940909E-4 5 0.014348709512938181 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003010666728161136 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001409248255735 24-25 0.0 26-27 5.124539111763636E-4 28-29 0.0014733049946320453 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.046241278675E-4 42-43 0.015245503857496815 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.405673889704545E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016654752113231816 84-85 1.281134777940909E-4 86-87 0.0 88-89 0.0 90-91 0.01140209952367409 92-93 0.0027544397725729544 94-95 0.0 96-97 0.0 98-99 0.0014733049946320453 100-101 0.019473248624701817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 780558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.36737143791646 #Duplication Level Percentage of deduplicated Percentage of total 1 84.44341920048818 62.79835121170392 2 10.591387516795292 15.753072990088542 3 2.649978534429028 5.91215813917167 4 0.9899261872989413 2.9447283386792327 5 0.4651318261553664 1.7295315641646258 6 0.24243489580296432 1.0817547567414973 7 0.1386629567115803 0.7218399724514877 8 0.09611124034476011 0.5718032248062109 9 0.061610453999765905 0.4123626765353331 >10 0.2934815922297183 3.7593564612206345 >50 0.018167336898772528 0.9278354646244655 >100 0.008477226503980624 1.2503080592987885 >500 3.460092404598258E-4 0.2317148383445761 >1k 6.920184809196516E-4 1.2376994696695292 >5k 1.730046202299129E-4 0.667482832499534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5188 0.6646527227957435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.5387171741241522 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3099 0.3970236676838877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1274 0.1632165707096718 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1042 0.1334942438614427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 964 0.12350139259350362 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.10723098091365407 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.281134777940909E-4 0.0 12-13 0.0 0.0 0.0 1.281134777940909E-4 0.0 14-15 0.0 0.0 0.0 1.9217021669113636E-4 0.0 16-17 0.0 0.0 0.0 4.483971722793181E-4 0.0 18-19 0.0 0.0 0.0 6.405673889704545E-4 0.0 20-21 6.405673889704545E-5 0.0 0.0 7.686808667645453E-4 0.0 22-23 1.281134777940909E-4 0.0 0.0 0.001281134777940909 0.0 24-25 1.281134777940909E-4 0.0 0.0 0.001729531950220227 0.0 26-27 1.281134777940909E-4 0.0 0.0 0.0023700993391906813 0.0 28-29 1.281134777940909E-4 0.0 0.0 0.004035574550513863 0.0 30-31 1.281134777940909E-4 0.0 0.0 0.008647659751101135 0.0 32-33 1.281134777940909E-4 0.0 0.0 0.013964369079555908 0.0 34-35 1.281134777940909E-4 0.0 0.0 0.019217021669113633 0.0 36-37 1.281134777940909E-4 0.0 0.0 0.024533730997568406 0.0 38-39 1.281134777940909E-4 0.0 0.0 0.03644828443241886 0.0 40-41 1.281134777940909E-4 0.0 0.0 0.056626157184988174 0.0 42-43 1.281134777940909E-4 0.0 0.0 0.10460465461887522 0.0 44-45 1.281134777940909E-4 0.0 0.0 0.14944437184680703 0.0 46-47 1.281134777940909E-4 0.0 0.0 0.2448248560645077 0.0 48-49 1.281134777940909E-4 0.0 0.0 0.3416786452768404 0.0 50-51 1.281134777940909E-4 0.0 0.0 0.40720868916851793 0.0 52-53 1.281134777940909E-4 0.0 0.0 0.49330094624614695 0.0 54-55 1.281134777940909E-4 0.0 0.0 0.5816351891851727 0.0 56-57 1.281134777940909E-4 0.0 0.0 0.7731648384873385 0.0 58-59 1.281134777940909E-4 0.0 0.0 1.0046018361223636 0.0 60-61 1.281134777940909E-4 0.0 0.0 1.2293769329120963 0.0 62-63 1.281134777940909E-4 0.0 0.0 1.4683085689980757 0.0 64-65 1.281134777940909E-4 0.0 0.0 1.760407298368603 0.0 66-67 1.281134777940909E-4 0.0 0.0 2.1385342280778623 0.0 68-69 1.281134777940909E-4 0.0 0.0 2.5383123355343225 0.0 70-71 1.281134777940909E-4 0.0 0.0 2.829514270560291 0.0 72-73 1.281134777940909E-4 0.0 0.0 3.1265453688258913 0.0 74-75 1.281134777940909E-4 0.0 0.0 3.5004445537679456 0.0 76-77 1.281134777940909E-4 0.0 0.0 3.9555676836314535 0.0 78-79 1.281134777940909E-4 0.0 0.0 4.384363493808276 0.0 80-81 1.281134777940909E-4 0.0 0.0 4.933201632678161 0.0 82-83 1.281134777940909E-4 0.0 0.0 5.5453918863172245 0.0 84-85 1.281134777940909E-4 0.0 0.0 6.240215333133476 0.0 86-87 1.281134777940909E-4 0.0 0.0 6.937536992766713 0.0 88-89 1.281134777940909E-4 0.0 0.0 7.9013346862116585 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3220 0.0 35.700924 1 GTATCAA 5115 0.0 33.433117 1 CTACACG 80 4.4941079E-4 29.6855 4 TAGGACG 200 2.3646862E-11 28.498077 4 ATCAACG 6260 0.0 27.162704 3 TCAACGC 6305 0.0 26.968838 4 TATCAAC 6370 0.0 26.768211 2 GTAGGAC 340 0.0 26.542328 3 CAACGCA 6415 0.0 26.432356 5 AACGCAG 6555 0.0 25.86782 6 GGACGTG 245 1.0913936E-11 25.202381 6 AGGACGT 215 1.6607373E-9 24.300686 5 ACGCAGA 7210 0.0 23.321695 7 CGCAGAG 7235 0.0 23.175457 8 GTACATG 3960 0.0 22.791788 1 TACATGG 3925 0.0 22.75006 2 GAGTACT 4040 0.0 22.63297 12-13 AGTACTT 4200 0.0 22.618973 12-13 GTAGCCC 380 0.0 22.498482 3 TGTAGGA 675 0.0 21.813343 2 >>END_MODULE