FastQCFastQC Report
Thu 26 May 2016
SRR937789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666676
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65860.9878861695936257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48810.7321397500434994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.5198927215018989No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.16724765853278054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.14879791682916438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.11969832422346087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7470.11204843132196149No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42500.039.7022361
GGTATCA25250.038.2129171
TCAACGC53000.031.4637724
ATCAACG53050.031.4317533
TATCAAC53250.031.3160552
CAACGCA53950.030.9120545
AACGCAG55550.030.1027036
ACGCAGA60500.027.5612327
CGCAGAG60550.027.5384738
TCGTCCA700.00838405427.1461879
CCGCTCG350.00839317127.14210574-75
GTACATG29850.026.11411
TACATGG29300.025.9436992
GCAGAGT64150.025.4746769
GTGTAGC4800.024.755651
GAGTACT40800.024.21683512-13
ACATGGG29300.023.9961173
AGTACTT42800.023.58464812-13
GTATAGA2853.6379788E-1223.3484861
AGAGTAC56550.022.93384710-11
Produced by FastQC (version 0.11.5)