FastQCFastQC Report
Thu 26 May 2016
SRR937789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666676
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44170.6625407244298579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36720.5507922889079553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.38564460097558634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12100.1814974590355735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.13274814152601863No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8700.13049817302557765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.1103984544216381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.033.767821
GTATCAA43850.030.453111
ATCAACG52850.024.984273
TCAACGC53950.024.4748614
TATCAAC54400.024.2724022
CAACGCA55050.023.9858075
GTAGGAC3000.023.7485373
TAGGACG2202.1664164E-923.7485374
GTACATG33900.023.6909221
AACGCAG56100.023.5368756
TACATGG34150.023.2269742
GAGTACT36100.021.97562812-13
ACATGGG34000.021.9324723
ACGCAGA62550.020.9610867
CGCAGAG62500.020.901858
GTACTTT37500.020.77205514-15
AGTACTT37050.020.77106712-13
ATGCCGT2000.020.18928594-95
GTATAGA2651.841363E-819.7261241
CCCTTAG2702.282286E-819.3608251
Produced by FastQC (version 0.11.5)