##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 666676 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26362730921767 33.0 31.0 34.0 31.0 34.0 2 32.45653810846648 34.0 31.0 34.0 31.0 34.0 3 32.522441185823396 34.0 31.0 34.0 31.0 34.0 4 36.121333301333785 37.0 35.0 37.0 35.0 37.0 5 36.045623361272945 37.0 35.0 37.0 35.0 37.0 6 36.091962212529026 37.0 35.0 37.0 35.0 37.0 7 36.04275090148738 37.0 35.0 37.0 35.0 37.0 8 36.01903323353473 37.0 35.0 37.0 35.0 37.0 9 37.710013559810164 39.0 37.0 39.0 35.0 39.0 10-11 37.639867041861415 39.0 37.0 39.0 35.0 39.0 12-13 37.549550306295714 39.0 37.0 39.0 35.0 39.0 14-15 38.77852735061709 40.0 38.0 41.0 35.0 41.0 16-17 38.75644840972227 40.0 38.0 41.0 35.0 41.0 18-19 38.75762814320599 40.0 38.0 41.0 35.0 41.0 20-21 38.67929623985264 40.0 38.0 41.0 34.0 41.0 22-23 38.61257867389857 40.0 38.0 41.0 34.0 41.0 24-25 38.61576312931619 40.0 38.0 41.0 34.0 41.0 26-27 38.43660263756307 40.0 38.0 41.0 34.0 41.0 28-29 38.3984769213231 40.0 38.0 41.0 34.0 41.0 30-31 38.380077428915996 40.0 38.0 41.0 34.0 41.0 32-33 38.22580233876726 40.0 38.0 41.0 34.0 41.0 34-35 38.06579132892139 40.0 37.0 41.0 33.5 41.0 36-37 37.88747382536644 40.0 37.0 41.0 33.0 41.0 38-39 37.73809316669566 40.0 37.0 41.0 33.0 41.0 40-41 37.54210441053825 40.0 37.0 41.0 33.0 41.0 42-43 37.295508612879416 40.0 37.0 41.0 32.5 41.0 44-45 36.965282236048694 40.0 36.0 41.0 31.5 41.0 46-47 36.60238331663356 40.0 36.0 41.0 30.5 41.0 48-49 36.252501214982985 40.0 36.0 41.0 30.0 41.0 50-51 35.998245024569655 39.0 35.5 41.0 29.5 41.0 52-53 35.73986839184251 39.0 35.0 41.0 28.0 41.0 54-55 35.52051821274502 39.0 35.0 40.5 28.0 41.0 56-57 35.26159183771427 39.0 35.0 40.0 27.0 41.0 58-59 35.038999454007644 39.0 35.0 40.0 26.0 41.0 60-61 34.732143499991 38.0 34.5 40.0 26.0 41.0 62-63 34.358416982162254 38.0 34.0 40.0 25.0 41.0 64-65 34.02101320581512 38.0 34.0 40.0 23.0 41.0 66-67 33.63415662180729 37.0 33.5 40.0 22.0 41.0 68-69 33.174671054605234 36.5 33.0 39.0 21.5 40.5 70-71 32.766304771733196 36.0 32.5 39.0 19.5 40.0 72-73 32.294316129574185 35.5 32.0 38.5 18.0 40.0 74-75 31.81914678194505 35.0 31.5 38.0 15.0 40.0 76-77 30.48592719701924 34.0 29.5 36.0 13.0 39.0 78-79 31.05200427194019 35.0 31.0 37.0 9.5 39.0 80-81 30.855047279338088 35.0 31.0 36.0 7.5 39.0 82-83 30.44735073708968 34.0 31.0 36.0 4.5 37.5 84-85 30.01469154431838 34.0 30.5 35.5 2.0 37.0 86-87 29.66330946366751 34.0 30.0 35.0 2.0 36.5 88-89 29.260932596943643 34.0 29.5 35.0 2.0 36.0 90-91 28.784653264854292 34.0 29.0 35.0 2.0 36.0 92-93 28.365529132592144 34.0 29.0 35.0 2.0 35.0 94-95 27.913299713804008 34.0 29.0 35.0 2.0 35.0 96-97 27.441944062783122 33.5 27.0 35.0 2.0 35.0 98-99 26.70672710582052 33.0 25.5 35.0 2.0 35.0 100-101 24.42537079480887 31.0 20.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20771533734775005 1101 2 0.283387205464372 1101 3 0.21677681622838918 1101 4 0.14346865651372553 1101 5 0.14932992895222696 1101 6 0.13154506864601245 1101 7 0.0594843583566913 1101 8 0.18600117262883487 1101 9 0.26019606894887204 1101 10-11 0.2845940504596882 1101 12-13 0.29278588966550956 1101 14-15 0.4171663800414933 1101 16-17 0.35014266133513416 1101 18-19 0.4106082962366955 1101 20-21 0.3740952725885691 1101 22-23 0.3778318025810563 1101 24-25 0.32838008320786116 1101 26-27 0.23501656628811673 1101 28-29 0.3761161040731764 1101 30-31 0.5336725564402514 1101 32-33 0.4664728169776069 1101 34-35 0.48447784804393024 1101 36-37 0.5593389559612092 1101 38-39 0.6594015105677045 1101 40-41 0.5587220164224149 1101 42-43 0.7012370781435706 1101 44-45 0.5825015683070305 1101 46-47 0.5475541073735144 1101 48-49 0.4203306800174147 1101 50-51 0.5837131434610967 1101 52-53 0.5910308541402927 1101 54-55 0.6579528230166858 1101 56-57 0.6075827048335398 1101 58-59 0.7083986488487923 1101 60-61 0.8095408679858949 1101 62-63 0.7252104700312856 1101 64-65 0.8000928165467585 1101 66-67 0.8105811053522984 1101 68-69 0.8747658489294992 1101 70-71 0.7625787686779049 1101 72-73 0.6126128435727374 1101 74-75 0.7073491518088666 1101 76-77 0.4570373955472533 1101 78-79 0.6692423258627684 1101 80-81 0.835364518738313 1101 82-83 0.8121755360385663 1101 84-85 0.7105107482820898 1101 86-87 0.6487319347798888 1101 88-89 0.704795558757219 1101 90-91 0.6965903995304821 1101 92-93 0.8047339994816305 1101 94-95 0.8319994014422392 1101 96-97 0.9173179002911915 1101 98-99 0.8132991004562733 1101 100-101 1.0907965302581495 1102 1 0.06679545865043934 1102 2 0.082576323382888 1102 3 0.11405776086572672 1102 4 0.009297338445776404 1102 5 -0.023085430194555556 1102 6 0.006179915332893415 1102 7 0.12282472372975661 1102 8 0.05304874144759708 1102 9 0.22368879518277396 1102 10-11 0.05914209540614479 1102 12-13 0.09275072946685725 1102 14-15 0.14048162052260693 1102 16-17 0.15791819276689267 1102 18-19 0.2084756104370129 1102 20-21 0.23016919690624604 1102 22-23 0.18898747436060148 1102 24-25 0.15623684934859483 1102 26-27 0.2285514847604091 1102 28-29 0.2701689029714842 1102 30-31 0.14199307095115188 1102 32-33 0.2806683809325392 1102 34-35 0.16222583732006512 1102 36-37 0.20923494037351986 1102 38-39 0.3983681669793171 1102 40-41 0.45969881069130025 1102 42-43 0.4872363602895149 1102 44-45 0.573398006871912 1102 46-47 0.5693028877541266 1102 48-49 0.6039731300972591 1102 50-51 0.5842694803544077 1102 52-53 0.7979460594612036 1102 54-55 0.7050832380535326 1102 56-57 0.7392935414997623 1102 58-59 0.8222074506185209 1102 60-61 0.8245641616175021 1102 62-63 0.9094158495416806 1102 64-65 0.8087821468914811 1102 66-67 0.9547679525079786 1102 68-69 0.8911948180031786 1102 70-71 0.9172858402728536 1102 72-73 0.9000756689877001 1102 74-75 0.8009976084226444 1102 76-77 0.7680505952718342 1102 78-79 0.598341052770575 1102 80-81 0.8701628603489375 1102 82-83 0.9399693068233681 1102 84-85 0.8963327644264822 1102 86-87 1.1301900575182842 1102 88-89 1.1045014583455526 1102 90-91 1.0780357034464494 1102 92-93 0.9453301113496622 1102 94-95 1.0489872711737043 1102 96-97 1.2538918319339025 1102 98-99 1.2599548528112763 1102 100-101 1.6671831605926108 1103 1 0.06013685240186106 1103 2 0.10494690402503437 1103 3 0.1367203856415813 1103 4 0.010985353538622178 1103 5 0.1232432830005834 1103 6 0.2023225964148594 1103 7 -0.04991062003539071 1103 8 0.05802517348600844 1103 9 0.14858672575804377 1103 10-11 0.15746897456412512 1103 12-13 0.11111154415317515 1103 14-15 0.18575722257158844 1103 16-17 0.14336563358414622 1103 18-19 0.1446479047941409 1103 20-21 0.1642752790173745 1103 22-23 0.2009656645046931 1103 24-25 0.02253612449505482 1103 26-27 -0.04636134006567971 1103 28-29 -6.509233206557496E-4 1103 30-31 0.2017803460041634 1103 32-33 0.363198592898172 1103 34-35 0.2876368894382608 1103 36-37 0.34956360684159904 1103 38-39 0.48674802294314645 1103 40-41 0.44072762376553243 1103 42-43 0.6727369531391076 1103 44-45 0.7248316515242834 1103 46-47 0.38648764071399455 1103 48-49 0.5244772566808678 1103 50-51 0.6344908498077615 1103 52-53 0.36402542537497595 1103 54-55 0.7079394296812112 1103 56-57 0.7149954695751433 1103 58-59 0.6562708942861306 1103 60-61 0.7830836730860753 1103 62-63 0.6986622004502294 1103 64-65 0.6287340180182426 1103 66-67 0.7919840125989879 1103 68-69 0.8278329227535721 1103 70-71 0.5356206630312741 1103 72-73 0.5466613810205025 1103 74-75 0.746229111807434 1103 76-77 0.7544033729035853 1103 78-79 0.8224031530351645 1103 80-81 0.8647512825513033 1103 82-83 0.7654086872225356 1103 84-85 0.7398073303028809 1103 86-87 1.0395351777528532 1103 88-89 1.0589966016516108 1103 90-91 0.860348926620734 1103 92-93 1.0199809725878133 1103 94-95 0.9245428851380844 1103 96-97 0.8463135787082763 1103 98-99 0.6822506701551916 1103 100-101 1.1427653505731605 1104 1 0.16685181014716477 1104 2 0.14127067627986634 1104 3 0.11844748554027973 1104 4 0.11525522850913461 1104 5 0.015436225287196237 1104 6 0.056772175255595414 1104 7 -0.03820280881465976 1104 8 0.10432632664365826 1104 9 -0.046084603495529564 1104 10-11 0.05146520008901234 1104 12-13 0.21466121596810694 1104 14-15 0.17353319637228992 1104 16-17 0.12255051348090262 1104 18-19 0.13304486301125706 1104 20-21 0.001979787572381042 1104 22-23 0.14538166161833033 1104 24-25 0.10706077653562573 1104 26-27 0.03783582075193692 1104 28-29 0.10249701667628841 1104 30-31 0.06159883815686129 1104 32-33 -0.03581130122225318 1104 34-35 0.029063835305073837 1104 36-37 0.16691439712231926 1104 38-39 0.23396295987333815 1104 40-41 0.25567565534900183 1104 42-43 0.3506340709388098 1104 44-45 0.3397533172889666 1104 46-47 0.24348707176737605 1104 48-49 0.23090288425673577 1104 50-51 0.3160118800174345 1104 52-53 0.3099068069438786 1104 54-55 0.2008289846539455 1104 56-57 0.11527300450984512 1104 58-59 0.04929089067817216 1104 60-61 0.3701678910620174 1104 62-63 0.2859941183553971 1104 64-65 0.5296401695800697 1104 66-67 0.7337773045796112 1104 68-69 0.672048400876875 1104 70-71 0.6035258293440862 1104 72-73 0.6520067038630195 1104 74-75 0.6970797563823261 1104 76-77 0.5237440886661844 1104 78-79 0.6826337845659118 1104 80-81 0.5440624408433514 1104 82-83 0.44148795119988904 1104 84-85 0.5588635727573958 1104 86-87 0.6276240721924395 1104 88-89 0.5970556610857187 1104 90-91 0.8347782454112362 1104 92-93 0.8680784706103779 1104 94-95 0.5822056246854252 1104 96-97 0.8451971944334069 1104 98-99 0.8651864346945501 1104 100-101 1.3395931262694276 1105 1 0.17523134320460798 1105 2 0.12796961768336956 1105 3 0.17280736372917715 1105 4 0.06475716387785724 1105 5 0.19804802490123308 1105 6 0.09448899790442766 1105 7 0.029308652807038982 1105 8 0.12517030357540193 1105 9 0.08453214887767047 1105 10-11 0.27449887662029937 1105 12-13 0.12308648396368937 1105 14-15 0.006066354202445723 1105 16-17 0.2624492273251988 1105 18-19 0.3315300326209467 1105 20-21 0.3105958037246239 1105 22-23 0.3731829265797941 1105 24-25 0.25319059683078393 1105 26-27 0.2594717929540167 1105 28-29 0.08730297783505847 1105 30-31 0.22290484951250988 1105 32-33 0.1689239381053298 1105 34-35 0.21670201617001794 1105 36-37 0.08509905472380552 1105 38-39 0.2632924410606563 1105 40-41 0.22819676592716576 1105 42-43 0.4770610567832847 1105 44-45 0.4974451530447652 1105 46-47 0.44233031247816257 1105 48-49 0.32990117756270365 1105 50-51 0.3072575435644609 1105 52-53 0.47712154921077143 1105 54-55 0.30433607356935966 1105 56-57 0.15029035032175386 1105 58-59 0.2683244333511041 1105 60-61 0.3268178626171121 1105 62-63 0.1717114318182027 1105 64-65 0.48463029752635123 1105 66-67 0.45714451151347646 1105 68-69 0.46666171755228447 1105 70-71 0.26042120788436307 1105 72-73 0.2780064584560371 1105 74-75 0.326887781190738 1105 76-77 0.5874829756339572 1105 78-79 0.4029177873769356 1105 80-81 0.403312610955016 1105 82-83 0.1873779419413495 1105 84-85 0.2897569657320638 1105 86-87 0.6030298303327015 1105 88-89 0.610819646495159 1105 90-91 0.604350010222106 1105 92-93 0.48948465263543284 1105 94-95 0.2605651905381592 1105 96-97 0.3128793254584288 1105 98-99 0.4631361708820414 1105 100-101 0.9137896972645514 1106 1 -0.2615420199502907 1106 2 -0.2882205369808162 1106 3 -0.34876403570392966 1106 4 -0.17754655186075752 1106 5 -0.20466967602506259 1106 6 -0.08804147424687159 1106 7 -0.1794352545210458 1106 8 -0.16340958515640835 1106 9 -0.32298583901083333 1106 10-11 -0.27682375189727537 1106 12-13 -0.3728570098012085 1106 14-15 -0.3824896210118993 1106 16-17 -0.10535473809866147 1106 18-19 -0.34726746911982076 1106 20-21 -0.4934733626079577 1106 22-23 -0.42945142870473774 1106 24-25 -0.454148391346088 1106 26-27 -0.4198356293797971 1106 28-29 -0.5099758045449008 1106 30-31 -0.3134964474441446 1106 32-33 -0.5305200726135482 1106 34-35 -0.8753615093857903 1106 36-37 -0.5505925734350612 1106 38-39 -0.27748538126190425 1106 40-41 -0.44465048999670387 1106 42-43 -0.4399818961141193 1106 44-45 -0.4407981880956271 1106 46-47 -0.5289453330109666 1106 48-49 -0.3082064191991236 1106 50-51 -0.6368428510085806 1106 52-53 -0.8349797456358701 1106 54-55 -0.8940371039041111 1106 56-57 -0.967886384639101 1106 58-59 -0.8285376208200503 1106 60-61 -0.7948949079045562 1106 62-63 -1.0496644394426724 1106 64-65 -1.0930288504490946 1106 66-67 -0.7004104016593544 1106 68-69 -0.5173320508664006 1106 70-71 -0.6835895109981749 1106 72-73 -0.8019684323506233 1106 74-75 -1.0941782693511861 1106 76-77 -0.9736723285952174 1106 78-79 -1.152299579463456 1106 80-81 -0.9263018187427612 1106 82-83 -1.0582191574896456 1106 84-85 -1.1545544304656872 1106 86-87 -0.7383672817583928 1106 88-89 -0.9011511790135316 1106 90-91 -0.6024368570367713 1106 92-93 -0.6479653223960469 1106 94-95 -0.9234897683516898 1106 96-97 -1.272855909702887 1106 98-99 -1.0671390455704568 1106 100-101 -0.38877256579820596 1107 1 0.13682844030800823 1107 2 0.07693793864831378 1107 3 -0.013104852207355577 1107 4 0.013649888577255354 1107 5 0.11525751475243595 1107 6 0.11201053234062641 1107 7 0.0804243276926968 1107 8 0.07810322868890296 1107 9 0.21385216046909505 1107 10-11 0.09385320051669765 1107 12-13 -0.046566235191981775 1107 14-15 -0.05380798800504749 1107 16-17 -0.10168538442497521 1107 18-19 0.2200738385569352 1107 20-21 0.3280501414803396 1107 22-23 0.45913168071133725 1107 24-25 0.3093733214041947 1107 26-27 0.222106238812394 1107 28-29 0.34619708846599195 1107 30-31 0.2102676737889908 1107 32-33 0.05610701126151696 1107 34-35 0.04970566578972324 1107 36-37 -0.060574948980409715 1107 38-39 0.026774825030891236 1107 40-41 -0.19174678886123075 1107 42-43 -0.2880865449251928 1107 44-45 -0.04434908232001078 1107 46-47 -0.4540125081864943 1107 48-49 -0.37808304801654913 1107 50-51 -0.11481950582930267 1107 52-53 -0.38911715859705964 1107 54-55 -0.3653901419556007 1107 56-57 -0.32567003723509913 1107 58-59 -0.19023621375293231 1107 60-61 -0.014478277353568103 1107 62-63 -0.27056241985352614 1107 64-65 -0.07185061231670176 1107 66-67 0.12047024322232858 1107 68-69 0.18625206978887832 1107 70-71 0.16052074296732144 1107 72-73 0.06006242489128866 1107 74-75 0.17715887779856487 1107 76-77 0.18663797737687204 1107 78-79 0.21571203534108818 1107 80-81 0.20421304647349814 1107 82-83 0.49882195660702777 1107 84-85 0.8374351104317164 1107 86-87 0.7579055794706946 1107 88-89 0.6829405837208569 1107 90-91 0.7176709121695488 1107 92-93 0.4982454198535322 1107 94-95 0.4977274747213194 1107 96-97 0.672009884408812 1107 98-99 0.6186544882771621 1107 100-101 0.930605666555671 1108 1 0.12169635992579941 1108 2 0.08569372471713876 1108 3 -0.03113693816649743 1108 4 0.050606369903825055 1108 5 0.03374133234952126 1108 6 0.11375797159852397 1108 7 0.08139099621834589 1108 8 0.13387256670685588 1108 9 0.2001143135373411 1108 10-11 0.0849022217648212 1108 12-13 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0.09718289834970761 2316 66-67 0.14809383573108903 2316 68-69 0.03107601023572215 2316 70-71 0.11049262918580638 2316 72-73 0.0805133987815232 2316 74-75 -0.21719337586943865 2316 76-77 0.0370318106068801 2316 78-79 0.23435123220003007 2316 80-81 0.3901724953472687 2316 82-83 0.5398464516402584 2316 84-85 0.26319069834615405 2316 86-87 0.4578041776262367 2316 88-89 0.5656820859098879 2316 90-91 0.37675646937508844 2316 92-93 0.19385486416181053 2316 94-95 0.3005225132396063 2316 96-97 0.38071025877865594 2316 98-99 0.4512068832349456 2316 100-101 0.8391974304191301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 9.0 5 17.0 6 34.0 7 85.0 8 201.0 9 400.0 10 702.0 11 1110.0 12 1622.0 13 2431.0 14 3269.0 15 4415.0 16 6121.0 17 8198.0 18 7348.0 19 4757.0 20 3820.0 21 3673.0 22 3813.0 23 4127.0 24 4582.0 25 5207.0 26 6327.0 27 7166.0 28 8937.0 29 10995.0 30 13449.0 31 16870.0 32 21375.0 33 27819.0 34 38346.0 35 55228.0 36 85085.0 37 143896.0 38 149592.0 39 15645.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.884499301364706 21.284888616071765 15.199632597377455 21.63097948518608 2 14.826992422106091 22.515434783913022 40.738079666884666 21.91949312709622 3 18.623289273950164 26.73682568444042 30.875717739951643 23.764167301657775 4 12.081730855768019 18.796086854784033 38.65655880817668 30.465623481271265 5 12.112298302115192 39.78111551286801 34.83311407883862 13.273472106178177 6 29.1739915641181 39.16190173337573 18.05014729793783 13.613959404568336 7 26.54092842700202 35.08600879587686 21.358650978886295 17.014411798234825 8 24.933250934486917 37.12673022577684 20.216716965962476 17.723301873773767 9 25.911987232178753 17.556654206841106 21.34515116788365 35.186207393096495 10-11 23.44084682814441 28.837246278552097 27.955333625329242 19.76657326797425 12-13 24.711203980517425 26.589859400852323 28.58581828163931 20.11311833699094 14-15 21.47939928840996 28.362727921809096 27.54178941494819 22.61608337483275 16-17 19.783073036977484 31.546358350983084 28.319903521350703 20.35066509068873 18-19 19.528001607977487 29.781258062387128 31.48178425502043 19.208956074614957 20-21 21.078533945027065 28.538182483629544 31.430452837858414 18.952830733484983 22-23 20.273434453131095 29.14014171971656 31.138662722674553 19.44776110447779 24-25 19.862946918743138 29.26744025583642 31.524458657578798 19.34515416784165 26-27 20.05578488843022 29.416966166067667 31.333437333125335 19.193811612376777 28-29 19.580329895082475 29.856157464039367 31.33178283294571 19.231729807932453 30-31 19.89362148929915 29.565636081094866 31.46108454481637 19.079657884789615 32-33 19.712649022913677 29.67730951766675 31.102514564796092 19.507526894623474 34-35 19.87922169089633 29.822207489095153 31.136489089152754 19.162081730855768 36-37 19.88627116572055 29.664670937122896 31.038431856073473 19.410626041083077 38-39 19.71842394206481 30.013904805332725 31.102064571096005 19.165606681506457 40-41 20.099388832181223 29.95209806284776 30.902486895778974 19.04602620919204 42-43 19.71342109290372 30.189057159746625 30.49820109884504 19.599320648504616 44-45 20.070244016583768 30.18947734731714 30.209952060671153 19.530326575427946 46-47 20.0392694502277 30.246626547228338 29.880181677456513 19.83392232508745 48-49 19.99367008861876 30.234851712076033 29.851982072250987 19.91949612705422 50-51 20.225491843114195 30.653045857357995 29.27576513928805 19.845697160239755 52-53 20.271916193173293 30.82974338359263 28.93586989782143 19.962470525412645 54-55 20.040694430277973 30.770419214131 28.948619719323933 20.240266636267094 56-57 20.357264998290024 30.724519856722004 28.666548668318644 20.251666476669328 58-59 20.333490331135366 30.6198963214515 28.924095062669124 20.122518284744014 60-61 20.337015281786055 30.559897161439743 29.042593403692347 20.060494153081855 62-63 20.236291691916314 30.44102382566644 29.229565786078993 20.09311869633825 64-65 20.138343063197116 30.483773227174822 29.17189159351769 20.205992116110373 66-67 20.15439283850026 30.375924737053683 29.02076870923807 20.448913715207986 68-69 20.30116578367903 30.411474239360647 28.968494441077823 20.318865535882498 70-71 20.25144147981928 30.290275936136897 28.96826944422778 20.490013139816043 72-73 20.053444251780476 30.272276188133368 28.993769087232778 20.68051047285338 74-75 20.01039485447204 30.49112312427626 28.643748987514172 20.854733033737528 76-77 19.712949018713736 30.22607683492431 29.032768541240422 21.028205605121528 78-79 19.580575871937793 30.26065135088109 28.78729697784231 21.37147579933881 80-81 19.549751303481752 30.2188769357229 28.76577227918809 21.46559948160726 82-83 19.584025823638466 30.131203163155718 28.76067235058709 21.524098662618723 84-85 19.50823114711066 29.926950913113586 28.84831439607005 21.716503543705702 86-87 19.349654104842532 29.90373134776113 28.67719851922073 22.069416028175606 88-89 19.435077908909275 29.7561334141322 28.547375336745283 22.261413340213238 90-91 19.405005865780147 29.677472103789803 28.74989873653196 22.16762329389809 92-93 18.935996753974845 29.695730141908598 28.88144883122844 22.486824272888114 94-95 18.721012905819318 29.99065513082817 28.647573933964924 22.64075802938759 96-97 18.73751267482255 29.923156075814937 28.722122890279532 22.617208359082973 98-99 18.882083912706303 30.28976898580855 28.170351403770088 22.65779569771506 100-101 18.609404805393634 30.147315297042216 27.82518094675151 23.418098950812638 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 618.0 1 432.0 2 263.5 3 330.0 4 352.5 5 378.0 6 589.5 7 1042.5 8 1472.0 9 1556.5 10 1363.0 11 1225.0 12 1249.5 13 1278.5 14 1290.5 15 1270.5 16 1227.0 17 1254.0 18 1385.0 19 1522.0 20 1693.0 21 1972.5 22 2319.5 23 2861.5 24 3617.5 25 4431.0 26 5468.5 27 6876.0 28 8295.0 29 9873.5 30 11662.5 31 13559.0 32 15780.5 33 18048.0 34 20547.5 35 22619.5 36 24660.0 37 26189.0 38 26513.0 39 26546.5 40 26792.0 41 26847.0 42 26332.0 43 25973.5 44 25712.5 45 25452.0 46 24598.5 47 24055.0 48 24596.0 49 23342.5 50 20821.0 51 18650.5 52 16426.0 53 14560.5 54 12644.5 55 10951.0 56 9403.0 57 7861.0 58 6479.0 59 5296.0 60 4313.5 61 3386.5 62 2597.0 63 1797.0 64 1132.0 65 799.0 66 623.5 67 525.5 68 361.5 69 224.0 70 174.5 71 129.5 72 95.0 73 94.0 74 87.0 75 59.5 76 46.0 77 38.0 78 25.0 79 18.0 80 12.0 81 7.0 82 4.5 83 4.5 84 3.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05654920831108365 2 0.0 3 0.0 4 0.0 5 0.003449951700676191 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004349939100852588 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.74994750073499E-4 22-23 0.0011999832002351966 24-25 0.0 26-27 0.0011999832002351966 28-29 0.001424980050279296 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.74994750073499E-4 38-39 0.0 40-41 2.2499685004409939E-4 42-43 0.023699668204645134 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.4999790002939958E-4 86-87 0.0 88-89 0.0 90-91 0.01469979420288116 92-93 0.002174969550426294 94-95 0.0 96-97 0.0 98-99 0.001049985300205797 100-101 0.016874763753307452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 666676.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.82103669826675 #Duplication Level Percentage of deduplicated Percentage of total 1 85.49651546000976 64.8243443626733 2 10.048816203423822 15.2382332426787 3 2.3578279470364145 5.363188779013408 4 0.888411320187296 2.6944106924430633 5 0.40925980869855694 1.5515251487229453 6 0.22574135992112268 1.0269566360937652 7 0.1279712643023215 0.6792039748892938 8 0.09097183359861635 0.5518062987031446 9 0.06509690530231597 0.4442143361283445 >10 0.26444583135501887 3.5354441343412786 >50 0.015044515688599552 0.7920271761828834 >100 0.00851189341029518 1.1432241620205092 >500 5.938530282451242E-4 0.37387047216548147 >1k 7.918040376601656E-4 1.781550583943904 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4417 0.6625407244298579 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3672 0.5507922889079553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2571 0.38564460097558634 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1210 0.1814974590355735 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 885 0.13274814152601863 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 870 0.13049817302557765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.1103984544216381 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2499685004409939E-4 0.0 14-15 0.0 0.0 0.0 2.9999580005879916E-4 0.0 16-17 0.0 0.0 0.0 2.9999580005879916E-4 0.0 18-19 0.0 0.0 0.0 2.9999580005879916E-4 0.0 20-21 0.0 0.0 0.0 3.74994750073499E-4 0.0 22-23 0.0 0.0 0.0 6.749905501322981E-4 0.0 24-25 0.0 0.0 0.0 0.0011999832002351966 0.0 26-27 0.0 0.0 0.0 0.001874973750367495 0.0 28-29 0.0 0.0 0.0 0.0036749485507202896 0.0 30-31 0.0 0.0 0.0 0.009449867701852174 0.0 32-33 0.0 0.0 0.0 0.015149787902969359 0.0 34-35 0.0 0.0 0.0 0.021074704954130642 0.0 36-37 0.0 0.0 0.0 0.027974608355483023 0.0 38-39 0.0 0.0 0.0 0.041774415158187785 0.0 40-41 0.0 0.0 0.0 0.0677240518632739 0.0 42-43 0.0 0.0 0.0 0.11947332737341677 0.0 44-45 0.0 0.0 0.0 0.16469769423228076 0.0 46-47 0.0 0.0 0.0 0.26714625995236063 0.0 48-49 0.0 0.0 0.0 0.36816984562216126 0.0 50-51 0.0 0.0 0.0 0.43956884603615554 0.0 52-53 0.0 0.0 0.0 0.5378924695054269 0.0 54-55 0.0 0.0 0.0 0.6343411192243309 0.0 56-57 0.0 0.0 0.0 0.8374382758641379 0.0 58-59 0.0 0.0 0.0 1.0868847836130295 0.0 60-61 0.0 0.0 0.0 1.3391812514624795 0.0 62-63 0.0 0.0 0.0 1.599877601713576 0.0 64-65 0.0 0.0 0.0 1.9156231812754623 0.0 66-67 0.0 0.0 0.0 2.3253424452057674 0.0 68-69 0.0 0.0 0.0 2.770836208293084 0.0 70-71 0.0 0.0 0.0 3.080581871853794 0.0 72-73 0.0 0.0 0.0 3.4217520954706635 0.0 74-75 0.0 0.0 0.0 3.8256964402498363 0.0 76-77 0.0 0.0 0.0 4.319114532396547 0.0 78-79 0.0 0.0 0.0 4.7757831390360534 0.0 80-81 0.0 0.0 0.0 5.369399828402402 0.0 82-83 0.0 0.0 0.0 6.033590529732583 0.0 84-85 0.0 0.0 0.0 6.801804774733154 0.0 86-87 0.0 0.0 0.0 7.540019439727844 0.0 88-89 0.0 0.0 0.0 8.608904475337345 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2885 0.0 33.76782 1 GTATCAA 4385 0.0 30.45311 1 ATCAACG 5285 0.0 24.98427 3 TCAACGC 5395 0.0 24.474861 4 TATCAAC 5440 0.0 24.272402 2 CAACGCA 5505 0.0 23.985807 5 GTAGGAC 300 0.0 23.748537 3 TAGGACG 220 2.1664164E-9 23.748537 4 GTACATG 3390 0.0 23.690922 1 AACGCAG 5610 0.0 23.536875 6 TACATGG 3415 0.0 23.226974 2 GAGTACT 3610 0.0 21.975628 12-13 ACATGGG 3400 0.0 21.932472 3 ACGCAGA 6255 0.0 20.961086 7 CGCAGAG 6250 0.0 20.90185 8 GTACTTT 3750 0.0 20.772055 14-15 AGTACTT 3705 0.0 20.771067 12-13 ATGCCGT 200 0.0 20.189285 94-95 GTATAGA 265 1.841363E-8 19.726124 1 CCCTTAG 270 2.282286E-8 19.360825 1 >>END_MODULE