FastQCFastQC Report
Thu 26 May 2016
SRR937788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484084
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54711.1301757546210989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39270.8112228456218342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.591839432825708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.1879839036200329No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.18426554069128498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.12745721816874755No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5870.1212599466208344No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.10266813197709489No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATT250.00500795257.033234
GGTATCA21450.040.55231
ATACGAC500.001611910138.0142823
GTATCAA34000.037.6066861
AGTATCG600.003937452631.6851315
TATACGA600.00393944931.6818522
AGAGCGC650.00583299629.238738
ATCAACG43000.029.1737523
TATCAAC43700.028.818162
TCAACGC43550.028.7021454
CAACGCA43800.028.538325
AACGCAG44500.028.0806796
GTAGCCC3450.026.1692543
CTAGGAC2007.1304385E-1026.1348193
GTACATG21400.025.987461
TACATGG20950.025.632812
ACGCAGA49150.025.2306757
CGCAGAG49550.025.0269978
TAGCCCT5200.024.6778414
TAGGACC1351.3345909E-524.643994
Produced by FastQC (version 0.11.5)